JP2011160759A - Method for cultivating sclerotium of sparassis crispa - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for cultivating sclerotia of Sparassis Crispa enabling cultivation of large-size sclerotia of Sparassis Crispa to attain high yield harvestry thereof. <P>SOLUTION: The method for cultivating sclerotia of Sparassis Crispa includes: inoculating spawns of Sparassis Crispa in culture medium to spread and age the hyphae of the Sparassis Crispa in the culture medium followed by irradiating the hyphae with a light of not less than 10 lx to generate small-size sclerotia (primordia of the fruit bodies); and thereafter inhibiting the light irradiation to avoid formation of the fruit bodies so as to grow the sclerotia. In addition, the sclerotia are grown by continuously maintaining the concentration of carbon dioxide in cultivation atmosphere at 2,000 ppm or more, after formation of the small-size sclerotia (primordia of the fruit bodies). The sclerotia comprise the masses of hyphae differentiated from mycelia and are distinguished from the fruit bodies in terms of no existence of spores. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a method for cultivating Hanabiratake, an edible rice cake. More specifically, the present invention relates to a cultivation method that makes it possible to obtain larger-sized and higher yields of fungal nuclei.

  Conventionally, many kinds of moss are used for food, and moss is used as a raw material for herbal medicines by using pharmacological species, or as a raw material for extracting physiologically active ingredients. Yes. As one of the moss used in various applications, Hanabiratake is an interesting moth with a number of remarkable features.

  Hanabiratake is suitable for edible reasons because it has a unique flavor, texture, and appearance, and is used as a food material such as disclosed in Japanese Patent Application Laid-Open No. 2004-290159 as a raw material for boiled fish, Polysaccharides, particularly β-glucan content, which is attracting attention as having physiological activity, is remarkably high in moss, and its components are extracted with a solvent as disclosed in JP-A-2004-290157. It is also used for health foods. Furthermore, according to the research by the present inventor, Hanabiratake can be cut and kneaded with cereal powder, so that processed foods such as noodles can be preferably modified.

  However, Hanabiratake is cultivated under humid conditions, and its fruiting body is petal-like and thin, and has a beautiful characteristic appearance, but its surface area is extremely large, so it is represented on the surface of the fruiting body by yeast, mold, etc. It tends to cause fungal and bacterial contamination, making it difficult to grow.

  Hanabiratake is a slow growing cocoon and requires not only a long cultivation period to obtain fruit bodies, but also the weight of fruit bodies obtained per weight of the fungus bed is small. For this reason, a manufacturing cost becomes high, Therefore Even if it is an artificially cultivated product, it will become an expensive cocoon. Needless to say, when using foods, the cost of production is one of the greatest concerns, even when it is used as a raw material for extraction of bioactive ingredients such as β-glucan or as a raw material for thickening stabilizers. Yes, it is a big problem that Hanabira bamboo is an expensive cocoon.

The inventors of the present invention have attempted to solve such problems by using mycorrhizal nuclei as foods instead of the fruit bodies of Hanabiratake, the contents of which are disclosed in Japanese Patent Application Laid-Open No. 2009-050192. ing. That is, it is an invention in which not the fruit body of the petal-like and thin form but the bulky sclerotia that should be called the cocoon is used directly as food or the like. In addition, although fungal nuclei, such as agaricus, in this invention are mainly obtained by artificial cultivation, they are completely different from fruit bodies and mycelium. This is a mycelium mass differentiated from the mycelium and is distinguished from the fruiting body in that there are no spores. Furthermore, the fungal nuclei such as garlic mushrooms in the present invention have a volume of 100 cm ³ or more at the time of harvest, that is, before cutting or crushing, such as umbrellas, cocoons, limbs, bags, shells, petals, etc. Does not have a characteristic structure in the child entity. In addition, fruiting bodies are generated from the fungal nuclei and may take a form in which the fungal nuclei and the fruiting bodies are integrated. The fungal nuclei of the present invention, such as Hanabiratake, are the total weight of the fungal nuclei at the time of harvest (that is, The total weight of the fruiting body and mycelium is 20 to 100% by weight.

  More specifically, in general, agaricus bamboo grows and spreads in the fungus bed in a period of about 50 days after inoculating the fungus species on the fungus bed. Here, when light irradiation of about 10 lx or more is performed, sclerotia which is a fruit body primordium is formed in about 10 to 20 days. Normally, after this, by continuing light irradiation and appropriately managing and maintaining temperature and humidity, the mycorrhiza changes into a fruiting body and grows for about 40 to 100 days, and is harvested as Hanabira bamboo. However, since the generation and growth of the fruiting body requires strict management of temperature and humidity over a long period of time, there is a problem that a lot of cost is required. A certain fungal nucleus itself was intended to be used as food. In addition, hereinafter, the use of mycorrhiza as food means to extract various components including β-glucan and other physiologically active components in addition to the case where the fungus itself is directly edible. Is also included.

  As described above, the mycorrhiza, such as agaricus, is a lump of mycelium that stores nutrients, and is not a thin and wide area like a fruit body but a lump. Therefore, various bacteria such as fungi and bacteria are less likely to adhere and mix, and accidents such as the occurrence of mold during cultivation are unlikely to occur. In addition, the mycorrhiza is a form prior to the occurrence of fruiting bodies differentiated from mycelia. Therefore, harvesting the mycorrhiza means harvesting without going through the process of generating fruiting bodies. Therefore, it becomes a more aseptic cultivation process and cultivation becomes easier. This will be described more specifically in the embodiment. In addition, as clarified in the above-mentioned publication, the phyllodes fungus nuclei contain components that are extremely close to the fruit bodies of the flower moth, and can be used in the same manner as the fruit bodies of the flower moth.

  Although it is a Hanabiratake fungus nuclei having such excellent characteristics, Hanabiratake is still a slow growing and expensive moth, and even if it uses Hanabiratake fungus nuclei, it is still expensive even if it is not as much as the fruiting body I have to say. Therefore, there is a demand for a cultivation method capable of harvesting more Hanabiratake fungal nuclei at a lower cost.

In addition, although it is not about Hanabiratake, it is disclosed by Unexamined-Japanese-Patent No. 2000-092986 or Unexamined-Japanese-Patent No. 2006-008645 regarding cultivation of a mycorrhiza. The former discloses a method for artificial cultivation of sclerotia mycorrhiza as a traditional Chinese medicine raw material. However, this is not directly applicable to the cultivation of Hanabiratake fungal nuclei.
JP 2004-290159 A JP 2004-290157 A JP 2009-050192 A JP 2006-008645 A

  As described above, the problem to be solved by the present invention is to provide a method for cultivating agaricobacterial nuclei capable of obtaining a higher yield. In other words, the present invention provides a method for cultivating agaricus nuclei that makes it possible to cultivate larger gallicus fungus nuclei and increase the yield of edible fungal bacterium per weight of the fungus bed.

(1) In order to solve the above-described problems, the present invention provides a fungus bed inoculated with a species of genus Hanabiratake,
At least after the generation of fungal nuclei, the method includes a period during which the carbon dioxide concentration is maintained at 2000 ppm or more.

  As already explained, the direct use of the leaflet of the leaflet, not the fruit body of the leaflet, directly reduces the risk of production costs and contamination of germs and molds. . However, Hanabiratake fungus nuclei are usually relatively small tissues and have a low yield per fungus bed, so even if the technical difficulty of cultivation is reduced, they still have to be expensive foods.

  However, according to the research of the inventors of the present invention, it has been found that if the carbon dioxide concentration in the cultivation environment after the generation of fungal nuclei is 2000 ppm or more, the morphological change from the fungal nuclei to the fruiting bodies occurs. Yes. That is, the growth continues in the form of mycorrhiza, and it becomes possible to finally harvest huge fungi. When growing fruit bodies, it is necessary to secure sufficient space for the petal-like fruit body to grow, and to make every effort to control humidity and prevent contamination of germs and pests. However, when growing massive sclerotia, a relatively small space is sufficient for the growth, and strict humidity control as in the case of fruiting body formation is not required. In addition, since cultivation can be continued in a sealed state, there is little concern about contamination with germs and pests. Furthermore, at this time, the weight of the fungal nuclei that can be finally harvested from the same volume of the fungal bed is larger than that of the fruit body. Therefore, a large yield can be secured at a low cost.

  In addition, if the carbon dioxide concentration in the cultivation environment decreases after the generation of fungal nuclei, there is a risk that the fungal nuclei may differentiate into fruiting bodies. Therefore, at least after the occurrence of fungal nuclei, the concentration of carbon dioxide is continuously maintained at 2000 ppm or more. It is preferable. For example, after increasing the concentration of carbon dioxide in the cultivating atmosphere to 2000 ppm or more after the occurrence of sclerotia, this concentration may be maintained until just before the sclerotium grows and is harvested.

  However, even after the occurrence of mycorrhiza, when the carbon dioxide concentration in the cultivation environment is reduced to less than 2000 ppm, the fungus nucleus does not instantaneously differentiate into fruiting bodies. Therefore, the present invention does not prevent the occurrence of a period in which the carbon dioxide concentration in the cultivation environment is reduced to less than 2000 ppm, for example, from several hours to about a day after the occurrence of sclerotia. However, this should not continue for a longer period or this situation should not be repeated excessively. Specifically, after generating microbial nuclei in the mycelium in a room, when moving the mycelium to a dedicated room for growing mycelium, the dioxide in the air to which the mycelium is exposed during this movement. The situation where the carbon concentration is less than 2000 ppm is not a problem for the present invention.

  In order to generate sclerotia in the fungus bed, the fungus bed can be irradiated with light. Specifically, the mycelium is generated by performing light irradiation with an illuminance of 10 lx or more for several days to 20 days in a state where the mycelium grows and spreads on the fungus bed. However, the method for generating mycorrhiza is not limited to this, and any method can be used.

(2) In order to solve the above-described problem, the present invention provides a fungus bed inoculated with a species of genus Hanabiratake,
At least after the sclerotia generation, the period for which the carbon dioxide concentration is maintained in a state of 4000 ppm or more is included.

  According to the research of the present inventor, if the carbon dioxide concentration in the cultivation environment is maintained at 2000 ppm or more, the ganobacterial sclerotia will develop a deficiency in the morphological change to the fruiting body, and the growth continues with the sclerotia. It is clear that this phenomenon occurs without inconvenience even when the concentration of carbon dioxide in the cultivating environment is further increased and exceeds 4000 ppm.

  If the carbon dioxide concentration in the cultivation environment is set so high, the carbon dioxide concentration in the cultivation environment is still kept high enough even if minor damage (eg, so-called pinholes) exists in the cultivation container. As a result, the accident that the mycorrhiza differentiates into fruiting bodies is prevented. In addition, cultivation bottles and bags made of polypropylene, which have a relatively good gas barrier property, are usually used for grape cultivation, but such accidents are likely to occur especially in cultivation bags made of a thin film. However, when the carbon dioxide concentration is controlled to be 4000 ppm or more, such a problem substantially does not occur.

(3) In order to solve the above-described problem, the present invention provides a fungus bed inoculated with the species of genus Hanabiratake,
At least after the occurrence of fungal nuclei, the method for cultivating Hanabiratake fungal nuclei according to (1) or (2) is characterized in that aeration is restricted.

  As already explained, in the present invention, after inoculating the genus Hanabiratake, generating mycorrhiza in the mycelium on which the mycelium has grown and spread, and maintaining the high carbon dioxide concentration in the cultivation environment, Insufficiency occurs in the morphological changes to the fruiting bodies of the plant, and the sclerotia grows enormously. Here, the method of maintaining the carbon dioxide concentration high can be any method. For example, an appropriate amount of carbon dioxide may be introduced into the cultivation environment from a commercially available carbon dioxide cylinder through a regulator.

  However, in the present invention, the purpose is to cultivate massive sclerotia and not the purpose of obtaining petal-like fruit bodies, so the cultivation space can be greatly reduced compared to the case of fruit body cultivation. . Moreover, strict humidity control is not required as in the case of fruit body cultivation. For this reason, by maintaining the cultivation container in a substantially sealed state, it is possible to increase the carbon dioxide in the cultivation atmosphere to the required concentration by the carbon dioxide generated along with the respiration of the hyphae of the cultivated Hanabiratake. Is possible.

  Actually, at the stage where the inoculum is inoculated and sealed in the fungus bed packed in the cultivation container, naturally the carbon dioxide concentration in the cultivation atmosphere is almost equal to the carbon dioxide concentration in the atmosphere, and is considered to be about 380 ppm. Thereafter, as the mycelium grows and spreads on the fungus bed, the carbon dioxide concentration in the sealed cultivation container increases. If the size of the cultivation container and the amount of the fungus bed are appropriately selected, the carbon dioxide in the cultivation container can be set to a high concentration of 5000 ppm or more when the mycelium is generated.

  Even if such a setting is made, aerating is performed for strict humidity control after cultivation of the normal fruit body. Moreover, when using a cultivation bottle especially as a cultivation container, in order to ensure the space which a fruit body can fully grow, a cultivation bottle is open | released. For this reason, the carbon dioxide concentration of the cultivation atmosphere quickly approaches that of the atmosphere, but in the present invention, it is possible to keep the cultivation container sealed, and therefore the appropriate cultivation container and fungus bed By selecting the amount, the carbon dioxide concentration after the generation of the nuclei can be made appropriate by the concentration of carbon dioxide generated with the respiration of the mycelium.

  According to such a method, it is necessary to find an appropriate set value of the amount of the cultivation container and the fungus bed, but once the conditions are found, the cost of the Hanabiratake fungus nuclei is substantially reduced without cost. The remarkable effect that cultivation can be achieved is obtained. At the same time, there is no concern about introducing germs and pests from the outside into the cultivation container, and it is possible to achieve more hygienic and stable cultivation of the fungal nuclei.

(4) In order to solve the above-described problems, the present invention provides a fungus bed inoculated with the species of genus Hanabiratake,
A first step of performing light irradiation of an average of 10 lx or more for 1 hour or more;
A second step in which light irradiation to the fungus bed is limited to an average of less than 5 lx and left for 4 hours or more;
This is a method for cultivating the leaf of the fungal shoot.

  As already explained, it is possible to cultivate giant fungal nuclei by causing failure in the morphological change of fungal nuclei into fruiting bodies by maintaining a high carbon dioxide concentration in the cultivation atmosphere after the occurrence of fungal nuclei. The inventor of the present invention has further found that a failure in the morphological change from the sclerotia to the fruiting body can be caused by restricting light irradiation after the nuclei are generated. Even if this is utilized, it is possible to continue the growth in the form of the mycorrhiza and finally harvest the huge fungus. Naturally, as in the case of maintaining a high carbon dioxide concentration in the cultivation atmosphere, the advantage of harvesting mycorrhiza instead of fruiting bodies can be enjoyed.

  More specifically, after the first step of irradiating the fungus bed with an average of 10 lx or more of light irradiation for 1 hour or more after the generation of the sclerotia, the light irradiation to the fungus nuclei is limited to an average of less than 5 lx. Apply the second step, which is left for more than 4 hours. In the second step, there is no problem even if there is virtually no light (0lx) and it is technically and economically easy and advantageous. The effect of the process including the first step and the second step becomes more remarkable when it is continuously applied repeatedly. Although it is not always clear why the sclerotia can be enlarged at a high growth rate by such a process, the light irradiation in the first step promotes the generation of the sclerotia and the light in the second step. By limiting the irradiation, the morphological change from the sclerotia to the fruiting body is suppressed, and it is imagined that the sclerotia becomes enormous by repeating these steps.

  Here, the first step of irradiating light on average 10 lx or more for 1 hour or longer is essentially sufficient light stimulation for the mycelium of Hanabiratake (including fungal nuclei in addition to those grown on the fungus bed) Therefore, the light irradiation does not necessarily have to be completely continuously continued. For example, even if stronger light is irradiated intermittently, the request of the present invention is satisfied as long as light irradiation with an average of 10 lx or more is performed for 1 hour or more.

  Conversely, the second step of limiting light irradiation to an average of less than 5 lx is a process in which light stimulation that promotes differentiation into fruiting bodies is not given to the fungal nuclei of Hanabiratake. This is not to say that Therefore, even if a human being is exposed to an environment bright enough to visually check the fungus bed for the purpose of confirming the cultivation situation, this is not immediately an obstacle to the present invention. If the period during which light irradiation is limited to less than 5 lx on average continues for 4 hours or more, the request of the present invention is satisfied.

  The light source used for light irradiation is essentially a natural light source (sunlight) or an artificial light source, but it is preferable to use an artificial light source in terms of easy management of light irradiation intensity and time. The reason for this is that it is not affected by the weather, and it is difficult to cause variations in the intensity of light irradiation among strains during actual cultivation. In addition, virtually any artificial light source can be used, and so-called filament lamps, fluorescent lamps, light-emitting diodes, etc. can be used. However, Hanabira bamboo cultivation requires relatively strict temperature control and consumes less power. Since it is small and economical because it has a long life, it is preferable to use a fluorescent lamp or a light emitting diode that is cold and has high luminous efficiency.

(5) In order to solve the above-described problem, the present invention provides a fungus bed inoculated with the species of Hanabiratake,
A first step of performing light irradiation of an average of 10 lx or more for 1 hour or more;
A second step in which light irradiation to the fungus bed is limited to an average of less than 5 lx and left for 4 hours or more;
(1) thru | or (3) characterized by including it.

  As described above, after the mycelium grows and spreads in the fungus bed, the first step in which light irradiation of an average of 10 lx or more is applied to the fungus bed for an hour or more and the average is less than 5 lx and left for 4 hours or more. It is a method for cultivating agaricus fungus nuclei comprising the steps of 2, or at least after the occurrence of microbial nuclei, a method for cultivating agaricus sclerotia including a state in which the carbon dioxide concentration in the cultivation atmosphere is 2000 ppm or more, Normally, small sclerotia can grow enormously. The present invention is a combination of these two cultivation methods, and even if one of the conditions is damaged by an accident or the like, the giant fungal nuclei can be stably produced without the Hanabiratake fungal nuclei being differentiated into fruiting bodies. The effect that it can be cultivated is obtained.

  That is, even if light irradiation with an average illuminance of 10 lxl or more is performed on the fungus bed, light irradiation with uniform illumination intensity is performed on the entire fungus bed surface due to cultivation facilities and sometimes the shadow of the fungus itself. It is not easy. However, after the mycelium grows and spreads in the mycelium, light irradiation is always necessary to generate the mycelium, and if it is insufficient, the mycelium will not be generated. On the other hand, if light irradiation is performed excessively, there is a risk that the mycorrhiza will differentiate into fruiting bodies. However, in recent years, various devices are often equipped with a light emission source to show the operating state, and there are cases where it is desirable to install emergency exit guide lights in the cultivation facility, and it is uniform for all fungus beds during cultivation. It may be difficult to limit the light irradiation to below a certain illuminance. Furthermore, the carbon dioxide concentration in the cultivation environment may not satisfy the desired conditions partially due to, for example, a slight defect (typically pinhole) in the cultivation container or unintended air inflow or leakage due to gas permeation. Can happen.

  However, even if it is a method of limiting light irradiation or a method of maintaining a high concentration of carbon dioxide in the cultivation atmosphere, the effect is common in that the mycelium can finally grow enormously. If one of them is satisfied, the objective is achieved. Therefore, even if a fungus bed that does not satisfy one of the conditions is generated by cultivating the conditions related to light irradiation and the conditions related to the carbon dioxide concentration in the cultivation atmosphere basically satisfying both, the result As such, it is an advantage of the present invention that a huge sclerotia can be stably cultivated without the Hanabiratake sclerotia being differentiated into fruiting bodies.

  As described above, according to the method for cultivating Hanabiratake fungus nuclei according to the present invention, the following effects can be obtained.

(1) A method for cultivating agaricus fungus nuclei characterized by including a period in which the fungus bed inoculated with the fungal bacterium species is maintained in a state where the carbon dioxide concentration is at least 2000 ppm after the occurrence of fungal nuclei, Therefore, it is possible to continue to grow and to be harvested as a huge mycorrhiza while the morphological change of the leaflet of the fungus is suppressed. This eliminates the need for strict humidity control as in the case of growing the fruit body, and the space required for growth may be narrower. Furthermore, since cultivation can be continued in a sealed state, there is little concern about contamination with germs and pests. In addition, the yield of the leaflet of Hanabiratake is higher than that of the fruit body of the leaflet, and the edible part of the leaflet can be cultivated at a lower cost overall.

(2) Hanabiratake according to (1), characterized in that the fungus bed inoculated with the species of Hanabiratake includes a period in which the carbon dioxide concentration is maintained at 4000 ppm or higher after the occurrence of sclerotia. Since it was set as the cultivation method of a mycorrhiza, even if slight damage exists in a cultivation container, the form change from a fungal nucleus to a fruiting body is suppressed. Thereby, even if an inexpensive cultivation bag is used, it becomes possible to cultivate the phyllobacteria nuclei more reliably.

(3) Cultivation of Hanabiratake fungus nuclei as described in (2) or (3), wherein the fungus bed inoculated with the species of Hanabiratake is at least aerated after the occurrence of fungal nuclei Because it was a method, it is possible to increase the carbon dioxide concentration of the cultivation environment to the desired concentration without actively supplying carbon dioxide to the cultivation container, and it is possible to cultivate the fungal nuclei at a lower cost Become. Furthermore, there is no concern about introducing germs and pests from outside into the cultivation container, and a remarkable effect is obtained in that a more hygienic Hanabiratake fungus nuclei free of contamination and pests can be obtained.

(4) A first step of irradiating an average of 10 lx or more of light to the fungus bed inoculated with the species of Hanabiratake fungus for 1 hour or more, and limiting the light irradiation to the fungus bed to an average of less than 5 lx for 4 hours or more And the second step of leaving it, which is characterized in that it is a method for cultivating Hanabiratake fungus nuclei. It can be harvested, and the same effect as in the case of (1) or (3) can be obtained.

(5) A first step of irradiating an average of 10 lx or more of light on the fungus bed inoculated with the genus Hanabiratake fungus for 1 hour or more, and limiting the light irradiation to the fungus bed to an average of less than 5 lx for 4 hours or more The method for cultivating agaricus fungus nuclei as described in (1) to (3), characterized in that it comprises a second step to be left, so that the light irradiation condition to the fungus bed and the carbon dioxide concentration condition of the cultivation atmosphere Even if a case where the condition is not satisfied due to an accident or the like occurs, as long as either one of the conditions is satisfied, the growth of the algal nuclei continues to be suppressed while the morphological change to the fruiting body is suppressed. As a result, the effect of being able to cultivate the fungus mushroom nuclei of stable quality despite some accidents is obtained.

  Hereinafter, the method for cultivating Hanabiratake fungal nuclei according to the present invention will be described in detail by way of examples.

  Polypropylene cultivation bottle for cocoon cultivation (content 850 cc) and polypropylene cultivation bag for cocoon cultivation (content 2,000 cc) filled with sawdust added with various nutrients, pasteurized at high temperature, and inoculated Hanabiratake fungus species A fungus bed was created. Furthermore, these mouths were closed and cultured at a constant temperature for about 50 days, and mycelia were grown and spread in the cultivation container. At this point, it was observed that the fungus bed was almost entirely white due to the mycelium.

  Next, when the cultivation container was irradiated with light having an illuminance of 10 lx over a period of several days to about 20 days, generation of fungal nuclei was observed on the surface of the fungus bed. In addition, when using a cultivation bottle as a cultivation container, since there is almost no space in the cultivation bottle, it is virtually impossible to continue growing as it is, so open the mouth of the cultivation bottle to ensure ventilation, The fruit body was grown by adjusting the humidity and temperature. As a result, about 60 g of fruit bodies of Hanabiratake were finally harvested in a period of about 4 months after inoculation.

  In addition, fruit bodies were grown for comparison in an example using a straw cultivation bag as a cultivation container. In this example as well, the mouth of the cultivation bag is opened after the occurrence of fungal nuclei, the ventilation is secured, the fruit body is grown by adjusting the humidity and temperature, and finally, Hanabira Takeko in about 6 months after inoculation. About 210g of the entity could be harvested.

  On the other hand, in an example in which a straw bag was used as a cultivation container, cultivation of the leaflet of the fungus was applied using the method for cultivating the leaflet of the present invention. That is, the cultivation bag was sealed and the ventilation was restricted even after the generation of fungal nuclei, and the carbon dioxide concentration in the cultivation atmosphere was continuously maintained at 4000 ppm or more until immediately before harvesting.

  Further, this cultivation container was irradiated with light having an illuminance of 10 lx for 1 day, and then repeatedly left in dark conditions for 3 days. Thereby, the morphological change of the mycelium to the further sclerotia and the growth of the generated sclerotia were observed.

  As described above, according to the method for cultivating Hanabiratake fungus nuclei according to the present invention, growth continued for about 10 months after inoculation, and finally, about 540 g of Hanabiratake fungus nuclei could be harvested.

In addition, the graph shown below is the result of measuring the carbon dioxide concentration of the cultivation atmosphere throughout the cultivation period. Since the cultivation vessel is cultivated in an almost sealed manner, an increase in the carbon dioxide concentration that seems to be due to the respiration of the mycelia during growth of the mycelia is observed until about the 40th day, and at this point the carbon dioxide concentration is 5000 ppm or more. Has reached. After this, about 10 days, the cultivation container is irradiated with light with an illuminance of 10 lx to generate mycorrhiza, and after that, the mycelium is grown, but the cultivation container is almost sealed during this period. Therefore, it is clear that the carbon dioxide concentration in the cultivation atmosphere is continuously maintained at 4000 ppm or more until immediately before harvesting. In addition, although the carbon dioxide concentration has fallen gradually from the time when the cultivation days passed 60 days, this is imagined to be due to the minute leak of the cultivation container.

The above results are summarized as shown in the table below. The grape cultivation using the cultivation bottle is characterized by the fact that the cultivation bottle is strong and easy to handle, so it is suitable for automation. However, the cultivation bottle is relatively expensive and about 60 g per 850 cc fungus bed. The fruit body of Hanabiratake was only harvested. In the case of using a cheaper cultivation bag, about 210 g of the fruit body of Hanabiratake can be harvested with respect to a 2000 cc fungus bed, and a considerable improvement is seen. However, according to the method for cultivating Hanabiratake fungus nuclei according to the present invention, about 540 g of Hanabiratake fungal nuclei can be harvested with respect to a 2000 cc fungal bed, and a large amount of Hanabiratake edible parts are obtained. It has been shown.

In addition, since the resulting fungus nuclei are inoculated with the inoculum of the sterilized fungus in a heat-sterilized fungus bed, they are cultivated in a sealed state until just before harvest, It is expected that there will be almost no contamination with germs. Actually, the following table shows the results of the examination of the number of viable bacteria and fungi on the mycelial nuclei of Hanabiratake and the fruit bodies of Hanabiratake. It is clearly shown in the test results that miscellaneous fungi adhering to the mycelium of Hanabiratake are overwhelmingly less as miscellaneous fungi adhering to the fruit body.

  In the above, the cultivation bag was sealed and the carbon dioxide concentration was kept high even after the generation of fungal nuclei, and light irradiation with an illuminance of 10 lx was carried out for 1 day, and then it was repeatedly left in the dark for 3 days. However, only one of these, that is, even if the cultivation bag is sealed and the carbon dioxide concentration is kept high even after the occurrence of sclerotia, or light irradiation with an illuminance of 10 lx is carried out for one day, followed by dark conditions It is known that even if it is left alone for 3 days, there is a slight difference in the growth rate of mycorrhizal nuclei, and finally it is known that almost the same yield of ganobacterial sclerotia can be obtained and cultivation is performed. The person can appropriately design the cultivation process based on the judgment.

  As explained above, according to the cultivation method of the ganobacterial sclerotia according to the present invention, a highly sterile and hygienic sclerotia can be obtained, and more yield can be obtained from the same amount of microbial beds. Moreover, cultivation itself has the characteristics that it can implement without using special facilities, such as maintaining the sealing of a cultivation container or performing light irradiation only for desired time. Due to such characteristics, the present invention is extremely useful industrially, because it can provide an inexpensive edible portion.

Claims (5)

The fungus bed inoculated with the genus Hanabiratake is
A method for cultivating agaricobacterial nuclei characterized by including a period during which the carbon dioxide concentration is maintained at 2000 ppm or more after the occurrence of sclerotia. The fungus bed inoculated with the genus Hanabiratake is
2. The method for cultivating agaricus sclerotia according to claim 1, comprising at least a period during which the carbon dioxide concentration is maintained at 4000 ppm or more after the occurrence of sclerotia. The fungus bed inoculated with the genus Hanabiratake,
The method for cultivating agaricus sclerotia according to claim 1 or 2, wherein aeration is restricted at least after the occurrence of sclerotia. In the fungus bed inoculated with the genus Hanabiratake,
A first step of performing light irradiation of an average of 10 lx or more for 1 hour or more;
A second step in which light irradiation to the fungus bed is limited to an average of less than 5 lx and left for 4 hours or more;
A method for cultivating Hanabiratake fungus nuclei, comprising: In the fungus bed inoculated with the genus Hanabiratake,
A first step of performing light irradiation of an average of 10 lx or more for 1 hour or more;
A second step in which light irradiation to the fungus bed is limited to an average of less than 5 lx and left for 4 hours or more;
The method for cultivating agaricobacterial nuclei according to claim 1, comprising: