JP2010536551A5 - - Google Patents
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- Publication number
- JP2010536551A5 JP2010536551A5 JP2010521469A JP2010521469A JP2010536551A5 JP 2010536551 A5 JP2010536551 A5 JP 2010536551A5 JP 2010521469 A JP2010521469 A JP 2010521469A JP 2010521469 A JP2010521469 A JP 2010521469A JP 2010536551 A5 JP2010536551 A5 JP 2010536551A5
- Authority
- JP
- Japan
- Prior art keywords
- bilayer
- membrane
- hydrophilic
- amphiphilic molecules
- hydrated support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 23
- 108090000623 proteins and genes Proteins 0.000 claims 21
- 102000004169 proteins and genes Human genes 0.000 claims 21
- 239000012528 membrane Substances 0.000 claims 15
- 230000002209 hydrophobic effect Effects 0.000 claims 10
- 239000010410 layer Substances 0.000 claims 10
- 150000001875 compounds Chemical class 0.000 claims 8
- 239000003921 oil Substances 0.000 claims 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- 150000002632 lipids Chemical class 0.000 claims 6
- 239000012491 analyte Substances 0.000 claims 5
- 239000000463 material Substances 0.000 claims 5
- SNKAWJBJQDLSFF-NVKMUCNASA-N 1,2-dioleoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC SNKAWJBJQDLSFF-NVKMUCNASA-N 0.000 claims 4
- FHQVHHIBKUMWTI-ZCXUNETKSA-N 1-palmitoyl-2-oleoyl phosphatidylethanolamine Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCCN)OC(=O)CCCCCCC\C=C/CCCCCCCC FHQVHHIBKUMWTI-ZCXUNETKSA-N 0.000 claims 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims 4
- 230000036571 hydration Effects 0.000 claims 4
- 238000006703 hydration reaction Methods 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 claims 4
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 claims 4
- 108090000862 Ion Channels Proteins 0.000 claims 3
- 102000004310 Ion Channels Human genes 0.000 claims 3
- 239000000523 sample Substances 0.000 claims 3
- 239000002356 single layer Substances 0.000 claims 3
- UKDDQGWMHWQMBI-UHFFFAOYSA-O 1,2-diphytanoyl-sn-glycero-3-phosphocholine Chemical compound CC(C)CCCC(C)CCCC(C)CCCC(C)CC(=O)OCC(COP(O)(=O)OCC[N+](C)(C)C)OC(=O)CC(C)CCCC(C)CCCC(C)CCCC(C)C UKDDQGWMHWQMBI-UHFFFAOYSA-O 0.000 claims 2
- RZRNAYUHWVFMIP-KTKRTIGZSA-N 1-oleoylglycerol Chemical group CCCCCCCC\C=C/CCCCCCCC(=O)OCC(O)CO RZRNAYUHWVFMIP-KTKRTIGZSA-N 0.000 claims 2
- PAZGBAOHGQRCBP-DDDNOICHSA-N 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C/CCCCCCCC PAZGBAOHGQRCBP-DDDNOICHSA-N 0.000 claims 2
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims 2
- 229920000936 Agarose Polymers 0.000 claims 2
- 229930186217 Glycolipid Natural products 0.000 claims 2
- 239000000020 Nitrocellulose Substances 0.000 claims 2
- 239000004677 Nylon Substances 0.000 claims 2
- 239000004695 Polyether sulfone Substances 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- 229930182558 Sterol Natural products 0.000 claims 2
- 150000001335 aliphatic alkanes Chemical class 0.000 claims 2
- 150000001336 alkenes Chemical class 0.000 claims 2
- 239000007864 aqueous solution Substances 0.000 claims 2
- 239000000969 carrier Substances 0.000 claims 2
- 229920002301 cellulose acetate Polymers 0.000 claims 2
- 235000012000 cholesterol Nutrition 0.000 claims 2
- 230000003247 decreasing effect Effects 0.000 claims 2
- 150000002190 fatty acyls Chemical group 0.000 claims 2
- 239000000499 gel Substances 0.000 claims 2
- 239000011521 glass Substances 0.000 claims 2
- RZRNAYUHWVFMIP-HXUWFJFHSA-N glycerol monolinoleate Natural products CCCCCCCCC=CCCCCCCCC(=O)OC[C@H](O)CO RZRNAYUHWVFMIP-HXUWFJFHSA-N 0.000 claims 2
- 150000002313 glycerolipids Chemical class 0.000 claims 2
- 150000002327 glycerophospholipids Chemical class 0.000 claims 2
- 239000000017 hydrogel Substances 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 229940074096 monoolein Drugs 0.000 claims 2
- 229920001220 nitrocellulos Polymers 0.000 claims 2
- 229920001778 nylon Polymers 0.000 claims 2
- 150000003904 phospholipids Chemical class 0.000 claims 2
- 229920002401 polyacrylamide Polymers 0.000 claims 2
- 229920000515 polycarbonate Polymers 0.000 claims 2
- 239000004417 polycarbonate Substances 0.000 claims 2
- 229920006393 polyether sulfone Polymers 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- 229930001119 polyketide Natural products 0.000 claims 2
- 150000003881 polyketide derivatives Chemical class 0.000 claims 2
- 229920001296 polysiloxane Polymers 0.000 claims 2
- 150000003135 prenol lipids Chemical class 0.000 claims 2
- 150000003313 saccharo lipids Chemical class 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 239000000377 silicon dioxide Substances 0.000 claims 2
- 150000003408 sphingolipids Chemical class 0.000 claims 2
- -1 sterol lipid Chemical class 0.000 claims 2
- 235000003702 sterols Nutrition 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- 108010078791 Carrier Proteins Proteins 0.000 claims 1
- 102000014914 Carrier Proteins Human genes 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
- 108091005703 transmembrane proteins Proteins 0.000 claims 1
- 102000035160 transmembrane proteins Human genes 0.000 claims 1
- 230000032895 transmembrane transport Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0716264.7A GB0716264D0 (en) | 2007-08-21 | 2007-08-21 | Bilayers |
| GB0716264.7 | 2007-08-21 | ||
| PCT/GB2008/002805 WO2009024775A1 (en) | 2007-08-21 | 2008-08-19 | Bilayers |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010536551A JP2010536551A (ja) | 2010-12-02 |
| JP2010536551A5 true JP2010536551A5 (enExample) | 2011-09-29 |
| JP5797405B2 JP5797405B2 (ja) | 2015-10-21 |
Family
ID=38566709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010521469A Active JP5797405B2 (ja) | 2007-08-21 | 2008-08-19 | 二分子層 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20110041978A1 (enExample) |
| EP (1) | EP2183594B1 (enExample) |
| JP (1) | JP5797405B2 (enExample) |
| CN (1) | CN101821626B (enExample) |
| CA (1) | CA2697024C (enExample) |
| GB (1) | GB0716264D0 (enExample) |
| WO (1) | WO2009024775A1 (enExample) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2347775T3 (da) | 2005-12-13 | 2020-07-13 | Harvard College | Skabeloner til celletransplantation |
| NZ579083A (en) | 2007-02-20 | 2012-07-27 | Oxford Nanopore Tech Ltd | Lipid bilayer sensor system |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| GB0913823D0 (en) * | 2009-08-07 | 2009-09-16 | Isis Innovation | Bilayers |
| US8313956B2 (en) * | 2010-03-09 | 2012-11-20 | Nokia Corporation | Apparatus and associated methods |
| CN107648668B (zh) | 2010-10-06 | 2021-06-18 | 哈佛学院董事会 | 用于基于材料的细胞疗法的可注射的成孔水凝胶 |
| KR101939420B1 (ko) | 2011-02-11 | 2019-01-16 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 세공 |
| WO2012142174A1 (en) | 2011-04-12 | 2012-10-18 | Electronic Biosciences Inc. | Site specific chemically modified nanopore devices |
| US9675561B2 (en) | 2011-04-28 | 2017-06-13 | President And Fellows Of Harvard College | Injectable cryogel vaccine devices and methods of use thereof |
| GB201119032D0 (en) | 2011-11-03 | 2011-12-14 | Isis Innovation | Multisomes: encapsulated droplet networks |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| CN104244929B (zh) | 2012-04-16 | 2017-04-05 | 哈佛学院董事会 | 用于调节免疫反应的介孔二氧化硅组合物 |
| WO2013192178A1 (en) * | 2012-06-18 | 2013-12-27 | Electronic Biosciences Inc. | Cell-free assay device and methods of use |
| GB201219196D0 (en) | 2012-10-25 | 2012-12-12 | Isis Innovation | Droplet assembly method |
| GB201219201D0 (en) | 2012-10-25 | 2012-12-12 | Isis Innovation | Hydrogel network |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| US9823235B2 (en) | 2012-10-26 | 2017-11-21 | Oxford Nanopre Technologies Ltd. | Droplet interfaces |
| KR102284661B1 (ko) * | 2012-12-07 | 2021-08-02 | 옥스포드 유니버시티 이노베이션 리미티드 | 3d 프린팅에 의한 비말 어셈블리 |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN103435269A (zh) * | 2013-08-21 | 2013-12-11 | 哈尔滨工业大学 | 一种固体支撑仿生膜体系的制备方法 |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| US10682400B2 (en) | 2014-04-30 | 2020-06-16 | President And Fellows Of Harvard College | Combination vaccine devices and methods of killing cancer cells |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| EP3169800B1 (en) | 2014-07-14 | 2020-11-18 | Oxford University Innovation Limited | Measurement of analytes with membrane channel molecules, and bilayer arrays |
| US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| EP3250250A4 (en) | 2015-01-30 | 2019-05-22 | President and Fellows of Harvard College | PERITUMORAL AND INTRATUMORAL MATERIALS FOR CANCER THERAPY |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| JP7094533B2 (ja) | 2015-04-10 | 2022-07-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 免疫細胞捕捉デバイスおよびその製造および使用方法 |
| EP3283887B1 (en) | 2015-04-14 | 2021-07-21 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| WO2017004504A1 (en) | 2015-07-02 | 2017-01-05 | The University Of Massachusetts | Membrane and droplet-interface bilayer systems and methods |
| US10481120B1 (en) | 2015-08-06 | 2019-11-19 | University Of Tennessee Research Foundation | Droplet based measurement of specific capacitance and interfacial tensions |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
| EP3411475B1 (en) | 2016-02-06 | 2025-08-27 | President and Fellows of Harvard College | Recapitulating the hematopoietic niche to reconstitute immunity |
| US10371610B2 (en) | 2016-02-23 | 2019-08-06 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| KR20170099738A (ko) | 2016-02-23 | 2017-09-01 | 노을 주식회사 | 접촉식 염색 패치 및 그 제조 방법 |
| KR102478639B1 (ko) | 2016-02-23 | 2022-12-19 | 노을 주식회사 | 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치 |
| KR102222192B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| US10465240B2 (en) | 2016-03-30 | 2019-11-05 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
| US10233486B2 (en) | 2016-03-30 | 2019-03-19 | Roche Sequencing Solutions, Inc. | Method for inline bilayer capacitance monitoring |
| US10155979B2 (en) | 2016-03-30 | 2018-12-18 | Roche Molecular Systems, Inc. | Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus |
| JP7364333B2 (ja) | 2016-04-06 | 2023-10-18 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 変異体ポア |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| CN115537372A (zh) | 2016-07-13 | 2022-12-30 | 哈佛学院院长等 | 抗原呈递细胞模拟支架及其制备和使用方法 |
| EP3493842A4 (en) | 2016-08-02 | 2020-07-29 | President and Fellows of Harvard College | BIOMATERIALS TO MODULATE IMMUNE RESPONSES |
| GB201615741D0 (en) | 2016-09-15 | 2016-11-02 | Univ College Cardiff Consultants Ltd | Artificial cells |
| FR3061440B1 (fr) * | 2017-01-05 | 2021-02-12 | Centre Nat Rech Scient | Procede de fabrication d’une membrane multicouche sur support solide a base de copolymere a blocs amphiphile |
| CN108329471A (zh) * | 2017-01-19 | 2018-07-27 | 天津大学 | 一种锂硫电池正极活性材料异戊烯醇-硫共聚物及制备方法 |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| JP7058858B2 (ja) * | 2017-10-19 | 2022-04-25 | 地方独立行政法人神奈川県立産業技術総合研究所 | 脂質二重膜形成器具及びそれを用いた脂質二重膜形成方法 |
| DE102017126732A1 (de) * | 2017-11-14 | 2019-05-16 | Endress+Hauser Conducta Gmbh+Co. Kg | Sensormembran, Sensor, ein Messgerät umfassend den Sensor und Verwendung des Sensors |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
| WO2020061129A1 (en) | 2018-09-19 | 2020-03-26 | President And Fellows Of Harvard College | Compositions and methods for labeling and modulation of cells in vitro and in vivo |
| KR20210138594A (ko) | 2019-03-12 | 2021-11-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법 |
| JP7730005B2 (ja) | 2019-05-29 | 2025-08-27 | ナンジン、ユニバーシティ | 電極を使用しないナノポアによる分析物の検出 |
| CN112831395B (zh) * | 2019-11-25 | 2024-01-16 | 深圳华大生命科学研究院 | 用于纳米孔测序的类细胞膜 |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| WO2022013551A1 (en) | 2020-07-17 | 2022-01-20 | Oxford Nanopore Technologies Limited | Nanopore sensing device |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH067737A (ja) * | 1992-06-30 | 1994-01-18 | Junjiro Aoki | 累積膜の連続製造方法 |
| EP1373550A1 (en) * | 2001-03-14 | 2004-01-02 | Biacore AB | Method of preparing supported lipid film membranes and use thereof |
| JP4394916B2 (ja) * | 2003-09-19 | 2010-01-06 | 独立行政法人科学技術振興機構 | 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用 |
| JP4394917B2 (ja) * | 2003-09-19 | 2010-01-06 | 独立行政法人科学技術振興機構 | 人工脂質二重膜を有する電流測定装置 |
| JP2005114576A (ja) * | 2003-10-08 | 2005-04-28 | Hitachi Software Eng Co Ltd | 両親媒性分子固定化ビーズ、その製造方法、及びキャピラリビーズアレイのビーズ配列方法 |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| WO2006110350A2 (en) | 2005-03-29 | 2006-10-19 | The Board Of Trustees Of The Leland Stanford Junior University | Method of fabricating lipid bilayer membranes on solid supports |
| DK2179780T5 (da) | 2005-05-20 | 2013-05-27 | Aquaporin As | Membran til filtering af vand |
| JP5114702B2 (ja) * | 2005-07-29 | 2013-01-09 | 国立大学法人 東京大学 | 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置 |
| WO2007030019A1 (en) | 2005-09-07 | 2007-03-15 | Agresearch Limited | A method of manufacture |
| GB0614835D0 (en) * | 2006-07-26 | 2006-09-06 | Isis Innovation | Formation of bilayers of amphipathic molecules |
-
2007
- 2007-08-21 GB GBGB0716264.7A patent/GB0716264D0/en not_active Ceased
-
2008
- 2008-08-19 CN CN200880111401.7A patent/CN101821626B/zh active Active
- 2008-08-19 WO PCT/GB2008/002805 patent/WO2009024775A1/en not_active Ceased
- 2008-08-19 EP EP08788371.6A patent/EP2183594B1/en active Active
- 2008-08-19 US US12/733,275 patent/US20110041978A1/en not_active Abandoned
- 2008-08-19 CA CA2697024A patent/CA2697024C/en active Active
- 2008-08-19 JP JP2010521469A patent/JP5797405B2/ja active Active
-
2012
- 2012-10-16 US US13/652,481 patent/US8784929B2/en active Active
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