JP2010535150A5 - - Google Patents
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- JP2010535150A5 JP2010535150A5 JP2009551103A JP2009551103A JP2010535150A5 JP 2010535150 A5 JP2010535150 A5 JP 2010535150A5 JP 2009551103 A JP2009551103 A JP 2009551103A JP 2009551103 A JP2009551103 A JP 2009551103A JP 2010535150 A5 JP2010535150 A5 JP 2010535150A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- multiplex
- variable region
- population
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 48
- 210000004027 cell Anatomy 0.000 claims 40
- 230000003321 amplification Effects 0.000 claims 27
- 238000003199 nucleic acid amplification method Methods 0.000 claims 27
- 210000004698 lymphocyte Anatomy 0.000 claims 13
- 239000002773 nucleotide Substances 0.000 claims 9
- 125000003729 nucleotide group Chemical group 0.000 claims 9
- 238000010839 reverse transcription Methods 0.000 claims 8
- 108010005832 Leukosialin Proteins 0.000 claims 6
- 102000005931 Leukosialin Human genes 0.000 claims 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 102000003705 Syndecan-1 Human genes 0.000 claims 6
- 108090000058 Syndecan-1 Proteins 0.000 claims 6
- 238000007403 mPCR Methods 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 6
- 238000003757 reverse transcription PCR Methods 0.000 claims 6
- 239000013598 vector Substances 0.000 claims 6
- 102000017095 Leukocyte Common Antigens Human genes 0.000 claims 5
- 108010013709 Leukocyte Common Antigens Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical group 0.000 claims 5
- 241000283984 Rodentia Species 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 101000608935 Homo sapiens Leukosialin Proteins 0.000 claims 3
- 108060003951 Immunoglobulin Proteins 0.000 claims 3
- 102100039564 Leukosialin Human genes 0.000 claims 3
- 239000012634 fragment Substances 0.000 claims 3
- 102000018358 immunoglobulin Human genes 0.000 claims 3
- 210000004988 splenocyte Anatomy 0.000 claims 3
- 101000998953 Homo sapiens Immunoglobulin heavy variable 1-2 Proteins 0.000 claims 2
- 102000009786 Immunoglobulin Constant Regions Human genes 0.000 claims 2
- 108010009817 Immunoglobulin Constant Regions Proteins 0.000 claims 2
- 102100036887 Immunoglobulin heavy variable 1-2 Human genes 0.000 claims 2
- 108091081021 Sense strand Proteins 0.000 claims 2
- 230000000692 anti-sense effect Effects 0.000 claims 2
- 210000001185 bone marrow Anatomy 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 108091033319 polynucleotide Proteins 0.000 claims 2
- 102000040430 polynucleotide Human genes 0.000 claims 2
- 239000002157 polynucleotide Substances 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 101001008255 Homo sapiens Immunoglobulin kappa variable 1D-8 Proteins 0.000 claims 1
- 101001047628 Homo sapiens Immunoglobulin kappa variable 2-29 Proteins 0.000 claims 1
- 101001008321 Homo sapiens Immunoglobulin kappa variable 2D-26 Proteins 0.000 claims 1
- 101001047619 Homo sapiens Immunoglobulin kappa variable 3-20 Proteins 0.000 claims 1
- 101001008263 Homo sapiens Immunoglobulin kappa variable 3D-15 Proteins 0.000 claims 1
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims 1
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims 1
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 claims 1
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 claims 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 claims 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 claims 1
- 102100022964 Immunoglobulin kappa variable 3-20 Human genes 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 102000023732 binding proteins Human genes 0.000 claims 1
- 108091008324 binding proteins Proteins 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 210000000265 leukocyte Anatomy 0.000 claims 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000005087 mononuclear cell Anatomy 0.000 claims 1
- 210000003720 plasmablast Anatomy 0.000 claims 1
- 210000004180 plasmocyte Anatomy 0.000 claims 1
- 238000011830 transgenic mouse model Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200700316 | 2007-03-01 | ||
| DKPA200700316 | 2007-03-01 | ||
| US90477207P | 2007-03-05 | 2007-03-05 | |
| US60/904,772 | 2007-03-05 | ||
| PCT/DK2008/050048 WO2008104184A2 (en) | 2007-03-01 | 2008-02-27 | Method for cloning cognate antibodies |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010535150A JP2010535150A (ja) | 2010-11-18 |
| JP2010535150A5 true JP2010535150A5 (https=) | 2011-04-07 |
| JP5669397B2 JP5669397B2 (ja) | 2015-02-12 |
Family
ID=38561767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009551103A Active JP5669397B2 (ja) | 2007-03-01 | 2008-02-27 | 同族抗体をクローニングするための方法 |
Country Status (23)
| Country | Link |
|---|---|
| US (2) | US8283294B2 (https=) |
| EP (1) | EP2121920B1 (https=) |
| JP (1) | JP5669397B2 (https=) |
| KR (1) | KR20090118993A (https=) |
| CN (1) | CN101622346A (https=) |
| AT (1) | ATE521704T1 (https=) |
| AU (1) | AU2008221119B2 (https=) |
| BR (1) | BRPI0808171A2 (https=) |
| CA (1) | CA2677771A1 (https=) |
| CY (1) | CY1112664T1 (https=) |
| DK (1) | DK2121920T3 (https=) |
| ES (1) | ES2369946T3 (https=) |
| HR (1) | HRP20110686T1 (https=) |
| IL (1) | IL199773A0 (https=) |
| MX (1) | MX2009008908A (https=) |
| NZ (1) | NZ578851A (https=) |
| PL (1) | PL2121920T3 (https=) |
| PT (1) | PT2121920E (https=) |
| RU (1) | RU2459868C2 (https=) |
| SI (1) | SI2121920T1 (https=) |
| TW (1) | TW200911986A (https=) |
| WO (1) | WO2008104184A2 (https=) |
| ZA (1) | ZA200905831B (https=) |
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| WO2010022736A2 (en) | 2008-08-29 | 2010-03-04 | Symphogen A/S | Recombinant anti-epidermal growth factor receptor antibody compositions |
| US8628927B2 (en) | 2008-11-07 | 2014-01-14 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| EP2364368B1 (en) * | 2008-11-07 | 2014-01-15 | Sequenta, Inc. | Methods of monitoring conditions by sequence analysis |
| US9365901B2 (en) | 2008-11-07 | 2016-06-14 | Adaptive Biotechnologies Corp. | Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia |
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| US9506119B2 (en) | 2008-11-07 | 2016-11-29 | Adaptive Biotechnologies Corp. | Method of sequence determination using sequence tags |
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| US7850965B2 (en) | 2005-12-05 | 2010-12-14 | Symphogen A/S | Anti-orthopoxvirus recombinant polyclonal antibody |
| WO2007101441A1 (en) | 2006-03-06 | 2007-09-13 | Symphogen A/S | Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections |
| BRPI0808551B1 (pt) | 2007-03-01 | 2022-04-05 | Symphogen A/S | Composições de anticorpo para o receptor do fator de crescimento antiepidérmico recombinante, molécula de ligação bi-específica e composição farmacêutica que os compreende |
| JP2010534057A (ja) | 2007-03-06 | 2010-11-04 | シムフォゲン・アクティーゼルスカブ | 呼吸器合胞体ウイルス感染症を治療するための組換え抗体 |
| MX2009012526A (es) | 2007-05-25 | 2009-12-09 | Symphogen As | Metodo para fabricar una proteina policlonal recombinante. |
| CA2695309A1 (en) | 2007-09-07 | 2009-03-12 | Symphogen A/S | Methods for recombinant manufacturing of anti-rsv antibodies |
| CN102137928A (zh) | 2008-08-29 | 2011-07-27 | 西福根有限公司 | 克隆禽源抗体的方法 |
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2008
- 2008-02-27 DK DK08706942.3T patent/DK2121920T3/da active
- 2008-02-27 MX MX2009008908A patent/MX2009008908A/es active IP Right Grant
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- 2008-02-27 RU RU2009136342/10A patent/RU2459868C2/ru not_active IP Right Cessation
- 2008-02-27 AT AT08706942T patent/ATE521704T1/de active
- 2008-02-27 KR KR1020097020517A patent/KR20090118993A/ko not_active Ceased
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- 2008-02-27 AU AU2008221119A patent/AU2008221119B2/en active Active
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