CN103074349B - 巢式一步法rt-pcr从单个b细胞扩增鼠源抗体重链和轻链可变区基因全序列的方法 - Google Patents
巢式一步法rt-pcr从单个b细胞扩增鼠源抗体重链和轻链可变区基因全序列的方法 Download PDFInfo
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WO2015096063A1 (en) | 2013-12-25 | 2015-07-02 | Coyote Bioscience Co., Ltd. | Methods and systems for nucleic acid amplification |
CN112359099A (zh) * | 2013-12-25 | 2021-02-12 | 卡尤迪生物科技(北京)有限公司 | 用于核酸扩增的方法和系统 |
CN106086009B (zh) * | 2016-06-17 | 2020-03-17 | 中国农业科学院哈尔滨兽医研究所 | 一种利用单个b细胞pcr技术生产全猪源单克隆抗体的方法 |
CN107779500A (zh) * | 2017-10-25 | 2018-03-09 | 上海药明生物技术有限公司 | 一种快速获取大鼠杂交瘤细胞单克隆抗体序列的测序方法及引物序列 |
CN113061183B (zh) * | 2018-02-12 | 2022-06-21 | 深圳市国创纳米抗体技术有限公司 | 一种抗绿脓杆菌外毒素a的纳米抗体3c11 |
CN108997493B (zh) * | 2018-08-15 | 2019-07-09 | 中国农业科学院兰州兽医研究所 | 一种全牛源o型口蹄疫病毒广谱中和抗体的制备方法 |
CN109972209B (zh) * | 2019-01-18 | 2022-05-03 | 南开大学 | 基于乳液pcr的一步法将单链抗体库重排为全长抗体库的方法 |
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WO2008104184A2 (en) * | 2007-03-01 | 2008-09-04 | Symphogen A/S | Method for cloning cognate antibodies |
CN101294308A (zh) * | 2007-10-22 | 2008-10-29 | 侯明 | 人源化重组噬菌体抗体库方法 |
CN101451134A (zh) * | 2007-11-29 | 2009-06-10 | 上海凯勃生物技术有限公司 | 从人微量b细胞中扩增人抗体重链及轻链基因片段的方法 |
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US7241598B2 (en) * | 2004-06-29 | 2007-07-10 | The Chinese University Of Hong Kong | Frame-shifting PCR for germline immunoglobulin genes retrieval and antibody engineering |
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WO2008104184A2 (en) * | 2007-03-01 | 2008-09-04 | Symphogen A/S | Method for cloning cognate antibodies |
CN101622346A (zh) * | 2007-03-01 | 2010-01-06 | 西福根有限公司 | 克隆关联抗体的方法 |
CN101294308A (zh) * | 2007-10-22 | 2008-10-29 | 侯明 | 人源化重组噬菌体抗体库方法 |
CN101451134A (zh) * | 2007-11-29 | 2009-06-10 | 上海凯勃生物技术有限公司 | 从人微量b细胞中扩增人抗体重链及轻链基因片段的方法 |
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