JP2014521316A5 - - Google Patents
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- Publication number
- JP2014521316A5 JP2014521316A5 JP2014520314A JP2014520314A JP2014521316A5 JP 2014521316 A5 JP2014521316 A5 JP 2014521316A5 JP 2014520314 A JP2014520314 A JP 2014520314A JP 2014520314 A JP2014520314 A JP 2014520314A JP 2014521316 A5 JP2014521316 A5 JP 2014521316A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- pcr
- different
- library
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 108060003951 Immunoglobulin Proteins 0.000 claims 8
- 102000018358 immunoglobulin Human genes 0.000 claims 8
- 238000000034 method Methods 0.000 claims 5
- 230000003321 amplification Effects 0.000 claims 4
- 239000000427 antigen Substances 0.000 claims 4
- 102000036639 antigens Human genes 0.000 claims 4
- 108091007433 antigens Proteins 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 238000012408 PCR amplification Methods 0.000 claims 3
- 210000003719 b-lymphocyte Anatomy 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 238000012216 screening Methods 0.000 claims 2
- 230000000392 somatic effect Effects 0.000 claims 2
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims 1
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 claims 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 238000002823 phage display Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161506749P | 2011-07-12 | 2011-07-12 | |
| US61/506,749 | 2011-07-12 | ||
| PCT/US2012/046431 WO2013009967A2 (en) | 2011-07-12 | 2012-07-12 | Identifying affinity-matured human antibodies |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014521316A JP2014521316A (ja) | 2014-08-28 |
| JP2014521316A5 true JP2014521316A5 (https=) | 2015-07-16 |
| JP6731702B2 JP6731702B2 (ja) | 2020-07-29 |
Family
ID=47506915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014520314A Active JP6731702B2 (ja) | 2011-07-12 | 2012-07-12 | 親和性成熟化ヒト抗体の同定 |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US9234030B2 (https=) |
| EP (2) | EP2731965A4 (https=) |
| JP (1) | JP6731702B2 (https=) |
| KR (1) | KR102139388B1 (https=) |
| CN (2) | CN103687873B (https=) |
| AU (2) | AU2012281078B2 (https=) |
| CA (1) | CA2841475C (https=) |
| IL (2) | IL230358B (https=) |
| MX (2) | MX361600B (https=) |
| RU (2) | RU2636045C2 (https=) |
| WO (1) | WO2013009967A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103687873B (zh) * | 2011-07-12 | 2016-10-19 | 埃克斯生物科技公司 | 鉴定亲和力成熟的人类抗体 |
| GB2525568B (en) | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| GB201316644D0 (en) * | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| US10533204B2 (en) | 2014-05-30 | 2020-01-14 | National University Corporatlon University of Toyama | Method for amplifying a T cell receptor (TCR) cDNA |
| WO2015187779A1 (en) | 2014-06-03 | 2015-12-10 | Xbiotech, Inc. | Compositions and methods for treating and preventing staphylococcus aureus infections |
| EP3950944A1 (en) | 2014-09-15 | 2022-02-09 | AbVitro LLC | High-throughput nucleotide library sequencing |
| US10975146B2 (en) | 2018-06-29 | 2021-04-13 | Cedars-Sinai Medical Center | Interleukin-1 inhibition for combination treatment of pancreatic cancer |
| US20200392578A1 (en) * | 2019-06-14 | 2020-12-17 | The Regents Of The University Of California | Methods of sequencing antibody chains from hybridomas and kits for practicing same |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE284897T1 (de) * | 2000-03-10 | 2005-01-15 | Large Scale Biology Corp | Selfantigen-impfstoffe zur behandlung von b-zell lymphomen und sonstigen krebsen |
| AU2002953381A0 (en) * | 2002-12-18 | 2003-01-09 | Diatech Pty Ltd | In vivo affinity maturation scheme |
| US20050032130A1 (en) * | 2003-07-29 | 2005-02-10 | Genentech, Inc. | Neutralizing antibody assay and uses therefor |
| TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| PE20071101A1 (es) | 2005-08-31 | 2007-12-21 | Amgen Inc | Polipeptidos y anticuerpos |
| JP5205597B2 (ja) * | 2007-06-01 | 2013-06-05 | 静岡県 | １細胞レベルでの抗体遺伝子の解析・同定方法 |
| NZ591543A (en) * | 2008-09-30 | 2012-11-30 | Abbott Lab | Improved antibody libraries |
| ES2570460T3 (es) | 2009-02-20 | 2016-05-18 | Hoffmann La Roche | Método de obtención de ácido nucleico que codifica inmunoglobulina |
| CN103687873B (zh) * | 2011-07-12 | 2016-10-19 | 埃克斯生物科技公司 | 鉴定亲和力成熟的人类抗体 |
-
2012
- 2012-07-12 CN CN201280034673.8A patent/CN103687873B/zh active Active
- 2012-07-12 WO PCT/US2012/046431 patent/WO2013009967A2/en not_active Ceased
- 2012-07-12 JP JP2014520314A patent/JP6731702B2/ja active Active
- 2012-07-12 US US13/547,497 patent/US9234030B2/en active Active
- 2012-07-12 CA CA2841475A patent/CA2841475C/en active Active
- 2012-07-12 EP EP12811637.3A patent/EP2731965A4/en not_active Withdrawn
- 2012-07-12 AU AU2012281078A patent/AU2012281078B2/en active Active
- 2012-07-12 RU RU2014102617A patent/RU2636045C2/ru active
- 2012-07-12 RU RU2017134418A patent/RU2762685C2/ru active
- 2012-07-12 KR KR1020147003416A patent/KR102139388B1/ko active Active
- 2012-07-12 CN CN201610954453.7A patent/CN107090459A/zh active Pending
- 2012-07-12 EP EP15196994.6A patent/EP3034513B1/en active Active
- 2012-07-12 MX MX2014000431A patent/MX361600B/es active IP Right Grant
-
2014
- 2014-01-07 IL IL230358A patent/IL230358B/en active IP Right Grant
- 2014-01-10 MX MX2018015450A patent/MX2018015450A/es unknown
-
2015
- 2015-11-12 US US14/939,454 patent/US9453217B2/en active Active
- 2015-11-13 US US14/940,537 patent/US9394536B2/en active Active
-
2016
- 2016-08-26 US US15/248,413 patent/US9670483B2/en active Active
-
2017
- 2017-05-02 US US15/584,360 patent/US20170233457A1/en not_active Abandoned
- 2017-08-03 AU AU2017210585A patent/AU2017210585B2/en active Active
-
2018
- 2018-11-13 IL IL262977A patent/IL262977B/en active IP Right Grant
-
2019
- 2019-11-19 US US16/688,666 patent/US20200071388A1/en not_active Abandoned
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