AU2012281078B2 - Identifying affinity-matured human antibodies - Google Patents
Identifying affinity-matured human antibodies Download PDFInfo
- Publication number
- AU2012281078B2 AU2012281078B2 AU2012281078A AU2012281078A AU2012281078B2 AU 2012281078 B2 AU2012281078 B2 AU 2012281078B2 AU 2012281078 A AU2012281078 A AU 2012281078A AU 2012281078 A AU2012281078 A AU 2012281078A AU 2012281078 B2 AU2012281078 B2 AU 2012281078B2
- Authority
- AU
- Australia
- Prior art keywords
- seq
- primers
- pcr
- region
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/515—Complete light chain, i.e. VL + CL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2017210585A AU2017210585B2 (en) | 2011-07-12 | 2017-08-03 | Identifying affinity-matured human antibodies |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161506749P | 2011-07-12 | 2011-07-12 | |
| US61/506,749 | 2011-07-12 | ||
| PCT/US2012/046431 WO2013009967A2 (en) | 2011-07-12 | 2012-07-12 | Identifying affinity-matured human antibodies |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2017210585A Division AU2017210585B2 (en) | 2011-07-12 | 2017-08-03 | Identifying affinity-matured human antibodies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012281078A1 AU2012281078A1 (en) | 2014-01-23 |
| AU2012281078B2 true AU2012281078B2 (en) | 2017-05-04 |
Family
ID=47506915
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012281078A Active AU2012281078B2 (en) | 2011-07-12 | 2012-07-12 | Identifying affinity-matured human antibodies |
| AU2017210585A Active AU2017210585B2 (en) | 2011-07-12 | 2017-08-03 | Identifying affinity-matured human antibodies |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2017210585A Active AU2017210585B2 (en) | 2011-07-12 | 2017-08-03 | Identifying affinity-matured human antibodies |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US9234030B2 (https=) |
| EP (2) | EP2731965A4 (https=) |
| JP (1) | JP6731702B2 (https=) |
| KR (1) | KR102139388B1 (https=) |
| CN (2) | CN107090459A (https=) |
| AU (2) | AU2012281078B2 (https=) |
| CA (1) | CA2841475C (https=) |
| IL (2) | IL230358B (https=) |
| MX (2) | MX361600B (https=) |
| RU (2) | RU2762685C2 (https=) |
| WO (1) | WO2013009967A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6731702B2 (ja) * | 2011-07-12 | 2020-07-29 | エックスバイオテク, インコーポレイテッドXbiotech, Inc. | 親和性成熟化ヒト抗体の同定 |
| US10119134B2 (en) | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| GB201316644D0 (en) * | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| WO2015182749A1 (ja) * | 2014-05-30 | 2015-12-03 | 国立大学法人富山大学 | ＴＣＲ ｃＤＮＡの増幅方法 |
| KR101753553B1 (ko) | 2014-06-03 | 2017-07-03 | 엑스바이오테크, 인크. | 스타필로코커스 아우레우스 감염의 치료 및 예방을 위한 조성물 및 방법 |
| ES2895750T3 (es) | 2014-09-15 | 2022-02-22 | Abvitro Llc | Secuenciación de alto rendimiento de colecciones de nucleótidos |
| US10975146B2 (en) | 2018-06-29 | 2021-04-13 | Cedars-Sinai Medical Center | Interleukin-1 inhibition for combination treatment of pancreatic cancer |
| US20200392578A1 (en) * | 2019-06-14 | 2020-12-17 | The Regents Of The University Of California | Methods of sequencing antibody chains from hybridomas and kits for practicing same |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE284897T1 (de) * | 2000-03-10 | 2005-01-15 | Large Scale Biology Corp | Selfantigen-impfstoffe zur behandlung von b-zell lymphomen und sonstigen krebsen |
| AU2002953381A0 (en) * | 2002-12-18 | 2003-01-09 | Diatech Pty Ltd | In vivo affinity maturation scheme |
| BRPI0412217A (pt) * | 2003-07-29 | 2006-08-22 | Genentech Inc | método de avaliação da eficácia de anticorpo, métodos de imunoterapia, método de detecção de anticorpos neutralizantes a anticorpo terapêutico e método de avaliação da eficácia de antagonista |
| TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| PE20071101A1 (es) | 2005-08-31 | 2007-12-21 | Amgen Inc | Polipeptidos y anticuerpos |
| JP5205597B2 (ja) * | 2007-06-01 | 2013-06-05 | 静岡県 | １細胞レベルでの抗体遺伝子の解析・同定方法 |
| RU2011117213A (ru) * | 2008-09-30 | 2012-11-10 | Эббот Лэборетриз (Us) | Улучшенные библиотеки антител |
| EP2398913B1 (en) | 2009-02-20 | 2016-03-30 | F. Hoffmann-La Roche AG | Method for obtaining immunoglobulin encoding nucleic acid |
| JP6731702B2 (ja) * | 2011-07-12 | 2020-07-29 | エックスバイオテク, インコーポレイテッドXbiotech, Inc. | 親和性成熟化ヒト抗体の同定 |
-
2012
- 2012-07-12 JP JP2014520314A patent/JP6731702B2/ja active Active
- 2012-07-12 KR KR1020147003416A patent/KR102139388B1/ko active Active
- 2012-07-12 RU RU2017134418A patent/RU2762685C2/ru active
- 2012-07-12 RU RU2014102617A patent/RU2636045C2/ru active
- 2012-07-12 CA CA2841475A patent/CA2841475C/en active Active
- 2012-07-12 AU AU2012281078A patent/AU2012281078B2/en active Active
- 2012-07-12 EP EP12811637.3A patent/EP2731965A4/en not_active Withdrawn
- 2012-07-12 US US13/547,497 patent/US9234030B2/en active Active
- 2012-07-12 EP EP15196994.6A patent/EP3034513B1/en active Active
- 2012-07-12 MX MX2014000431A patent/MX361600B/es active IP Right Grant
- 2012-07-12 WO PCT/US2012/046431 patent/WO2013009967A2/en not_active Ceased
- 2012-07-12 CN CN201610954453.7A patent/CN107090459A/zh active Pending
- 2012-07-12 CN CN201280034673.8A patent/CN103687873B/zh active Active
-
2014
- 2014-01-07 IL IL230358A patent/IL230358B/en active IP Right Grant
- 2014-01-10 MX MX2018015450A patent/MX2018015450A/es unknown
-
2015
- 2015-11-12 US US14/939,454 patent/US9453217B2/en active Active
- 2015-11-13 US US14/940,537 patent/US9394536B2/en active Active
-
2016
- 2016-08-26 US US15/248,413 patent/US9670483B2/en active Active
-
2017
- 2017-05-02 US US15/584,360 patent/US20170233457A1/en not_active Abandoned
- 2017-08-03 AU AU2017210585A patent/AU2017210585B2/en active Active
-
2018
- 2018-11-13 IL IL262977A patent/IL262977B/en active IP Right Grant
-
2019
- 2019-11-19 US US16/688,666 patent/US20200071388A1/en not_active Abandoned
Non-Patent Citations (7)
| Title |
|---|
| CAMPBELL M J et al: "Use of family specific leader region primers for PCR amplification of the human heavy chain variable region gene repertoire", MOLECULAR IMMUNOLOGY, Vol. 29, No. 2, 1 February 1992 (1992-02-01 ), pages 193-203 * |
| DUBROVSKAYA V V et al: , MOLECULAR BIOLOGY, Vol. 41, No. 1, 1 February2007 (2007-02-01), pages 157-167 * |
| GLANVILLE, J. et al, 'Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire' Proc. Natl. Acad. Soc. USA, December 1 2009, Vol. 106, No. 48, Pages 20216-20221. * |
| LARRICK J W et al: "Polymerase chain reaction using mixed primers: cloning of human monoclonal antibody variable region genes from single hybridoma cells", BIOTECHNOLOGY. Vol. 7, 1 September 1989 (1989-09-01 ), pages 934-938 * |
| LARRICK J W et al: BIOCHEMICAL AND BIOPHYSICAL RESEARCH, Vol. 160, No. 3, 15 May1989 (1989-05-15), pages 1250-1256 * |
| PANSRI P et al "A compact phage display human scFv library for selection of antibodies to a wide variety of antigens", BMC BIOTECHNOLOGY, Vol. 9, No. 6, 29 January 2009 (2009-01-29), pages 1-16 * |
| SZANKASI P et al: "Clinical Laboratory Analysis of lmmunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis", JOURNAL OF MOLECULAR DIAGNOSTICS, Vol. 12, No. 2, March 2010 (2010-03), pages 244-249 * |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2017210585B2 (en) | Identifying affinity-matured human antibodies | |
| AU2019203048B2 (en) | Express humanization of antibodies | |
| JP6250284B2 (ja) | ヒト化抗体 | |
| US20040086979A1 (en) | Humanized rabbit antibodies | |
| Kim et al. | Generation, diversity determination, and application to antibody selection of a human naïve fab library | |
| US20210079066A1 (en) | Radically diverse human antibody library | |
| TW202229328A (zh) | 抗原特異性抗體之鑑定及產生 | |
| HK1192265B (en) | Identifying affinity-matured human antibodies | |
| HK1192265A (en) | Identifying affinity-matured human antibodies |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| HB | Alteration of name in register |
Owner name: XBIOTECH INC. Free format text: FORMER NAME(S): XBIOTECH, INC. |