ES2570460T3 - Método de obtención de ácido nucleico que codifica inmunoglobulina - Google Patents

Método de obtención de ácido nucleico que codifica inmunoglobulina

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Publication number
ES2570460T3
ES2570460T3 ES10705301T ES10705301T ES2570460T3 ES 2570460 T3 ES2570460 T3 ES 2570460T3 ES 10705301 T ES10705301 T ES 10705301T ES 10705301 T ES10705301 T ES 10705301T ES 2570460 T3 ES2570460 T3 ES 2570460T3
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ES
Spain
Prior art keywords
chain reaction
primer
nucleic acid
polymerase chain
primers
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Active
Application number
ES10705301T
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English (en)
Inventor
Hans Willi Krell
Alexander Lifke
Valeria Lifke
Kairat Madin
Christian Weilke
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Método de producción de un fragmento Fab de inmunoglobulina que comprende las siguientes etapas: - proporcionar una célula única productora de inmunoglobulina, - obtener a partir de dicha célula el ácido nucleico que codifica los dominios variables de la cadena ligera y pesada de inmunoglobulina, que también codifica una parte del dominio constante de la cadena ligera y una parte del dominio CH1 de la cadena pesada, con un método que comprende las siguientes etapas: - realizar una primera reacción en cadena de la polimerasa con cuatro a seis cebadores 5' y un cebador 3', - realizar con el producto de la primera reacción en cadena de la polimerasa una segunda reacción en cadena de la polimerasa con trece a quince cebadores 5' y un cebador 3', por el cual en la segunda reacción en cadena de la polimerasa el cebador 3' es el mismo que en la primera reacción en cadena de la polimerasa y al menos un cebador 5' se cambia, y por el cual en la segunda reacción en cadena de la polimerasa el número de nucleótidos entre el extremo 5' de cada uno de los cebadores 5' y el extremo 3' del cebador 3' se reduce en comparación con el número de nucleótidos entre el extremo 5' de cada uno de los cebadores 5' y el extremo 3' del cebador 3' en la primera reacción en cadena de la polimerasa, cuando se une al ácido nucleico que va a amplificarse, - generar una matriz de expresión lineal que comprende el ácido nucleico obtenido, - traducir in vitro dicho ácido nucleico y así producir el fragmento Fab de inmunoglobulina.
ES10705301T 2009-02-20 2010-02-18 Método de obtención de ácido nucleico que codifica inmunoglobulina Active ES2570460T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09002396 2009-02-20
PCT/EP2010/001008 WO2010094475A1 (en) 2009-02-20 2010-02-18 Method for obtaining immunoglobulin encoding nucleic acid

Publications (1)

Publication Number Publication Date
ES2570460T3 true ES2570460T3 (es) 2016-05-18

Family

ID=40790896

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10705301T Active ES2570460T3 (es) 2009-02-20 2010-02-18 Método de obtención de ácido nucleico que codifica inmunoglobulina

Country Status (8)

Country Link
US (2) US9399670B2 (es)
EP (1) EP2398913B1 (es)
JP (2) JP5684734B2 (es)
CN (1) CN102325903B (es)
CA (1) CA2751669C (es)
ES (1) ES2570460T3 (es)
SG (1) SG173669A1 (es)
WO (1) WO2010094475A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9399670B2 (en) 2009-02-20 2016-07-26 Hoffmann-La Roche Inc. Method for obtaining immunoglobulin encoding nucleic acid
MX361600B (es) 2011-07-12 2018-12-11 Xbiotech Inc Identificación de anticuerpos humanos con maduración de afinidad.
JP2014527825A (ja) * 2011-09-21 2014-10-23 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ＴａｑＭａｎプローブと組み合わせたマルチプレックスＰＣＲにより単一の抗体産生細胞からＦａｂ断片を得るための方法
WO2016077795A1 (en) * 2014-11-14 2016-05-19 Illumina, Inc. Polymerases
EP3824104A1 (en) * 2018-07-18 2021-05-26 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
JP2023511200A (ja) * 2020-01-22 2023-03-16 ライフ テクノロジーズ コーポレーション 自己免疫疾患および免疫不全疾患における免疫レパートリーバイオマーカー

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DE3883899T3 (de) 1987-03-18 1999-04-22 Sb2, Inc., Danville, Calif. Geänderte antikörper.
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
US5202238A (en) 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US5571690A (en) 1991-05-08 1996-11-05 The University Of Virginia Patents Foundation Method for the cell-free synthesis of proteins
GB9613418D0 (en) 1996-06-26 1996-08-28 Crc Technology Ltd A cell-free system for initiation of DNA replication
EP0953055A1 (en) 1997-01-21 1999-11-03 Daniel Favre Production of biologically active polypeptides
RU2148649C1 (ru) 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
CA2365668C (en) 1999-03-17 2014-05-20 The Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
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Also Published As

Publication number Publication date
US20170107550A1 (en) 2017-04-20
CN102325903B (zh) 2015-06-03
JP2012518392A (ja) 2012-08-16
CA2751669A1 (en) 2010-08-26
SG173669A1 (en) 2011-09-29
EP2398913B1 (en) 2016-03-30
US20110306094A1 (en) 2011-12-15
CN102325903A (zh) 2012-01-18
WO2010094475A1 (en) 2010-08-26
US9399670B2 (en) 2016-07-26
CA2751669C (en) 2017-11-21
JP2014221069A (ja) 2014-11-27
JP5684734B2 (ja) 2015-03-18
EP2398913A1 (en) 2011-12-28

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