JP2010519897A5 - - Google Patents
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- Publication number
- JP2010519897A5 JP2010519897A5 JP2009551271A JP2009551271A JP2010519897A5 JP 2010519897 A5 JP2010519897 A5 JP 2010519897A5 JP 2009551271 A JP2009551271 A JP 2009551271A JP 2009551271 A JP2009551271 A JP 2009551271A JP 2010519897 A5 JP2010519897 A5 JP 2010519897A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- nucleic acid
- nucleotide
- enriching
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 125000003729 nucleotide group Chemical group 0.000 claims 29
- 239000002773 nucleotide Substances 0.000 claims 28
- 150000007523 nucleic acids Chemical class 0.000 claims 24
- 108020004707 nucleic acids Proteins 0.000 claims 23
- 102000039446 nucleic acids Human genes 0.000 claims 23
- 238000000034 method Methods 0.000 claims 22
- 239000000523 sample Substances 0.000 claims 19
- 230000003321 amplification Effects 0.000 claims 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims 15
- 230000008878 coupling Effects 0.000 claims 6
- 238000010168 coupling process Methods 0.000 claims 6
- 238000005859 coupling reaction Methods 0.000 claims 6
- 230000000295 complement effect Effects 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- 101710163270 Nuclease Proteins 0.000 claims 3
- 238000003776 cleavage reaction Methods 0.000 claims 3
- 238000009396 hybridization Methods 0.000 claims 3
- 238000002844 melting Methods 0.000 claims 3
- 230000008018 melting Effects 0.000 claims 3
- 238000010791 quenching Methods 0.000 claims 3
- 230000000171 quenching effect Effects 0.000 claims 3
- 230000007017 scission Effects 0.000 claims 3
- 238000006243 chemical reaction Methods 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 238000011144 upstream manufacturing Methods 0.000 claims 2
- 208000035657 Abasia Diseases 0.000 claims 1
- 239000004215 Carbon black (E152) Substances 0.000 claims 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 229930195733 hydrocarbon Natural products 0.000 claims 1
- 150000002430 hydrocarbons Chemical class 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 239000002105 nanoparticle Substances 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- -1 nucleoside triphosphate Chemical class 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 230000010076 replication Effects 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0703996.9 | 2007-03-01 | ||
| GB0703996A GB0703996D0 (en) | 2007-03-01 | 2007-03-01 | Nucleic acid detection |
| PCT/GB2008/000707 WO2008104794A2 (en) | 2007-03-01 | 2008-02-29 | Nucleic acid detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010519897A JP2010519897A (ja) | 2010-06-10 |
| JP2010519897A5 true JP2010519897A5 (cg-RX-API-DMAC7.html) | 2011-04-21 |
| JP5394937B2 JP5394937B2 (ja) | 2014-01-22 |
Family
ID=37965755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009551271A Expired - Fee Related JP5394937B2 (ja) | 2007-03-01 | 2008-02-29 | 核酸検出 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9340832B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2129797B8 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5394937B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN101680029B (cg-RX-API-DMAC7.html) |
| DK (1) | DK2129797T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2414286T3 (cg-RX-API-DMAC7.html) |
| GB (1) | GB0703996D0 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2008104794A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2071035A1 (en) * | 2007-12-13 | 2009-06-17 | Epigenomics AG | Facilitator and method for amplification |
| EP2271772B1 (en) | 2008-03-11 | 2014-07-16 | Sequenom, Inc. | Nucleic acid-based tests for prenatal gender determination |
| US8962247B2 (en) | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US20100285478A1 (en) | 2009-03-27 | 2010-11-11 | Life Technologies Corporation | Methods, Compositions, and Kits for Detecting Allelic Variants |
| JP2012511927A (ja) | 2008-12-17 | 2012-05-31 | ライフ テクノロジーズ コーポレーション | 対立遺伝子変種を検出するための方法、組成物、およびキット |
| CN101633957A (zh) * | 2009-06-26 | 2010-01-27 | 北大工学院绍兴技术研究院 | 用于检测小rna的方法及试剂盒 |
| DK2516680T3 (en) | 2009-12-22 | 2016-05-02 | Sequenom Inc | Method and kits to identify aneuploidy |
| CN102985560A (zh) * | 2010-03-30 | 2013-03-20 | 莫诺匡特私人有限公司 | 调控寡核苷酸功能性的方法 |
| CN101871007B (zh) * | 2010-05-07 | 2014-04-30 | 无锡锐奇基因生物科技有限公司 | 用标记探针进行检测和熔解曲线分析的方法 |
| CN107190056A (zh) * | 2010-07-29 | 2017-09-22 | 霍夫曼-拉罗奇有限公司 | 微生物核酸的定性和定量检测 |
| US9353406B2 (en) * | 2010-10-22 | 2016-05-31 | Fluidigm Corporation | Universal probe assay methods |
| EP2702168B1 (en) | 2011-04-29 | 2018-01-17 | Sequenom, Inc. | Quantification of a minority nucleic acid species |
| WO2012151328A2 (en) | 2011-05-02 | 2012-11-08 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| CN103635593B (zh) * | 2011-05-04 | 2018-03-20 | 生物概念股份有限公司 | 用于检测核酸序列变体的方法 |
| JP5299981B1 (ja) * | 2011-09-08 | 2013-09-25 | 独立行政法人理化学研究所 | プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法 |
| GB201116131D0 (en) * | 2011-09-19 | 2011-11-02 | Epistem Ltd | Probe |
| WO2013093432A1 (en) | 2011-12-19 | 2013-06-27 | David Kelly | Method for enriching and detection of variant target nucleic acids |
| CN102559661B (zh) * | 2012-01-18 | 2014-06-04 | 厦门基科生物科技有限公司 | 一种新型连接酶反应介导的扩增方法及用途 |
| WO2013119888A1 (en) | 2012-02-09 | 2013-08-15 | President And Fellows Of Harvard College | Selective nucleic acid amplification from nucleic acid pools |
| US9605313B2 (en) | 2012-03-02 | 2017-03-28 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| HK1206792A1 (en) | 2012-07-13 | 2016-01-15 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| ES2749463T3 (es) * | 2012-11-02 | 2020-03-20 | Life Technologies Corp | Captura, detección y cuantificación de ARN pequeño |
| US9222122B2 (en) | 2012-11-20 | 2015-12-29 | Src, Inc. | System and method for rapid detection and identification of nucleic acid labeled tags |
| EP3597774A1 (en) | 2013-03-13 | 2020-01-22 | Sequenom, Inc. | Primers for dna methylation analysis |
| GB201310584D0 (en) * | 2013-06-13 | 2013-07-31 | Base4 Innovation Ltd | Droplet storage method |
| CA2917627A1 (en) | 2013-07-18 | 2015-01-22 | President And Fellows Of Harvard College | Specific nucleic acid amplification with compounded selectivity |
| CN118421758A (zh) * | 2013-11-26 | 2024-08-02 | 杭州联川基因诊断技术有限公司 | 一种扩增靶核酸的引物的制备方法 |
| WO2015138774A1 (en) | 2014-03-13 | 2015-09-17 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| CN104677866A (zh) * | 2014-03-25 | 2015-06-03 | 中国科学院大连化学物理研究所 | 测定CP装载siRNA体系中siRNA释放的方法 |
| US9909167B2 (en) * | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| GB201412316D0 (en) | 2014-07-10 | 2014-08-27 | Momentum Bioscience Ltd | Detecting the absence of micro-organisms |
| US10563250B2 (en) | 2015-03-13 | 2020-02-18 | Life Technologies Corporation | Methods, compositions and kits for small RNA capture, detection and quantification |
| CN108138221B (zh) * | 2015-05-10 | 2021-11-05 | 康代思锐公司 | 用于检测核酸的超高灵敏度探针 |
| US20200087726A1 (en) * | 2015-05-10 | 2020-03-19 | Quandx Inc. | Ultra sensitive probes for detection of nucleic acid |
| CN105274096A (zh) * | 2015-09-07 | 2016-01-27 | 中国人民解放军第三军医大学第一附属医院 | 桥式序列区掺入错配碱基的桥式荧光探针及其应用和方法 |
| CN105506082B (zh) * | 2015-12-24 | 2019-04-09 | 杭州师范大学附属医院 | 利用链转移型引物扩增核酸及融合探针的方法 |
| CA3031586A1 (en) | 2016-07-27 | 2018-02-01 | The Board Of Trustees Of The Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| US10081829B1 (en) | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| US10527608B2 (en) | 2017-06-13 | 2020-01-07 | Genetics Research, Llc | Methods for rare event detection |
| CA3069938A1 (en) | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Isolation of target nucleic acids |
| US10947599B2 (en) | 2017-06-13 | 2021-03-16 | Genetics Research, Llc | Tumor mutation burden |
| CN107881218B (zh) * | 2017-11-23 | 2021-09-28 | 中国科学院合肥物质科学研究院 | 一种用于端粒酶活性检测的球形核酸荧光探针及其制备方法和用途 |
| CN108251502B (zh) * | 2017-12-27 | 2021-12-24 | 深圳华大基因股份有限公司 | 一种外周血游离肿瘤dna的富集方法、试剂盒及其应用 |
| CN110240999B (zh) * | 2018-03-09 | 2022-09-06 | 浙江品级基因科技有限公司 | 一种提高循环肿瘤dna检出率的检测装置及方法 |
| AU2019287163B2 (en) | 2018-06-12 | 2025-08-21 | Keygene N.V. | Nucleic acid amplification method |
| KR102589502B1 (ko) | 2018-07-19 | 2023-10-13 | 바이오피델리티 엘티디 | 개선된 폴리뉴클레오티드 서열 탐지 방법 |
| US11921049B2 (en) * | 2018-11-09 | 2024-03-05 | Shaofeng Ding | Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid |
| CN109828120B (zh) * | 2019-03-12 | 2021-07-30 | 南京大学 | 基于靶标激发金纳米粒子表面dna循环的蛋白质荧光分析方法 |
| WO2021076557A1 (en) * | 2019-10-15 | 2021-04-22 | University Of Florida Research Foundation, Incorporated | Aptamer based system to quantify anti-thrombin iii in blood |
| WO2022175655A1 (en) * | 2021-02-16 | 2022-08-25 | Biofidelity Ltd | Methods for polynucleotide detection |
| CN113234803A (zh) * | 2021-06-17 | 2021-08-10 | 上海理工大学 | 一种新型核酸扩增方法 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2650840B1 (fr) | 1989-08-11 | 1991-11-29 | Bertin & Cie | Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US6376178B1 (en) * | 1993-09-03 | 2002-04-23 | Duke University | Method of nucleic acid sequencing |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| ATE318327T1 (de) | 1996-06-04 | 2006-03-15 | Univ Utah Res Found | Fluoreszenz-donor-akzeptor paar |
| US6248518B1 (en) | 1996-10-29 | 2001-06-19 | Board Of Regents Of University Of Nebraska | Method for detecting point mutations in DNA utilizing fluorescence energy transfer |
| US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| GB9811415D0 (en) | 1998-05-28 | 1998-07-22 | Dedar Limited | Gasifiers |
| GB9902971D0 (en) * | 1999-02-11 | 1999-03-31 | Zeneca Ltd | Assay |
| AU3274500A (en) | 1999-03-18 | 2000-10-09 | Exiqon A/S | Detection of mutations in genes by specific lna primers |
| US20020025519A1 (en) * | 1999-06-17 | 2002-02-28 | David J. Wright | Methods and oligonucleotides for detecting nucleic acid sequence variations |
| EP1250452A1 (en) | 1999-12-02 | 2002-10-23 | DNA Sciences, Inc. | Methods for determining single nucleotide variations and genotyping |
| JP3638516B2 (ja) | 2000-09-28 | 2005-04-13 | 株式会社日立製作所 | 核酸検出方法および核酸検出キット |
| US6391592B1 (en) | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| JP2005504281A (ja) * | 2001-09-24 | 2005-02-10 | ワン ラムダ | 診断用プローブ検出システム |
| EP1481088B1 (en) | 2002-03-01 | 2008-09-17 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US20030211483A1 (en) | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
| WO2004003173A2 (en) | 2002-07-01 | 2004-01-08 | Cleveland State University | Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides |
| WO2004018626A2 (en) * | 2002-08-21 | 2004-03-04 | Epoch Biosciences, Inc. | Abasic site endonuclease assay |
| GB2395556A (en) * | 2002-11-22 | 2004-05-26 | Dynal Biotech Ltd | Nucleic acid probe |
| WO2004065628A1 (en) | 2003-01-21 | 2004-08-05 | Guoliang Fu | Quantitative multiplex detection of nucleic acids |
| US20040229253A1 (en) | 2003-02-18 | 2004-11-18 | Hyldig-Nielsen Jens J. | Compositions and methods for multiplex analysis of polynucleotides |
| US7851148B2 (en) * | 2003-10-13 | 2010-12-14 | Qiagen Gmbh | Method and kit for primer based multiplex amplification of nucleic acids employing primer binding tags |
| US20060183136A1 (en) | 2004-11-05 | 2006-08-17 | Genevieve Pont-Kingdon | Method for haplotyping and genotyping by melting curve analysis of hybridization probes |
| WO2007008997A2 (en) | 2005-07-12 | 2007-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Template specific inhibition of pcr |
| WO2007045890A1 (en) | 2005-10-21 | 2007-04-26 | Primerdesign Ltd | Probe |
| US20100009355A1 (en) | 2006-03-14 | 2010-01-14 | Kolodney Michael S | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
| GB0703997D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Methods for detecting nucleic sequences |
-
2007
- 2007-03-01 GB GB0703996A patent/GB0703996D0/en not_active Ceased
-
2008
- 2008-02-29 US US12/529,204 patent/US9340832B2/en not_active Expired - Fee Related
- 2008-02-29 DK DK08709576.6T patent/DK2129797T3/da active
- 2008-02-29 JP JP2009551271A patent/JP5394937B2/ja not_active Expired - Fee Related
- 2008-02-29 ES ES08709576T patent/ES2414286T3/es active Active
- 2008-02-29 WO PCT/GB2008/000707 patent/WO2008104794A2/en not_active Ceased
- 2008-02-29 CN CN200880012820.5A patent/CN101680029B/zh active Active
- 2008-02-29 EP EP08709576.6A patent/EP2129797B8/en not_active Not-in-force
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