JP2010505396A5 - - Google Patents
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- Publication number
- JP2010505396A5 JP2010505396A5 JP2009530744A JP2009530744A JP2010505396A5 JP 2010505396 A5 JP2010505396 A5 JP 2010505396A5 JP 2009530744 A JP2009530744 A JP 2009530744A JP 2009530744 A JP2009530744 A JP 2009530744A JP 2010505396 A5 JP2010505396 A5 JP 2010505396A5
- Authority
- JP
- Japan
- Prior art keywords
- stained
- electrophoresis
- photograph
- dye
- agarose gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011543 agarose gel Substances 0.000 description 15
- 238000001962 electrophoresis Methods 0.000 description 15
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 15
- 229960005542 ethidium bromide Drugs 0.000 description 15
- 210000003296 saliva Anatomy 0.000 description 12
- 239000000203 mixture Substances 0.000 description 9
- 239000013614 RNA sample Substances 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82856306P | 2006-10-06 | 2006-10-06 | |
| US86698506P | 2006-11-22 | 2006-11-22 | |
| US94977807P | 2007-07-13 | 2007-07-13 | |
| PCT/CA2007/001785 WO2008040126A1 (en) | 2006-10-06 | 2007-10-05 | Stabilizing compositions and methods for extraction of ribonucleic acid |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013229632A Division JP2014057590A (ja) | 2006-10-06 | 2013-11-05 | リボ核酸を抽出するための方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010505396A JP2010505396A (ja) | 2010-02-25 |
| JP2010505396A5 true JP2010505396A5 (https=) | 2012-02-16 |
Family
ID=39268089
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009530744A Pending JP2010505396A (ja) | 2006-10-06 | 2007-10-05 | リボ核酸を抽出するための安定化組成物および方法 |
| JP2013229632A Pending JP2014057590A (ja) | 2006-10-06 | 2013-11-05 | リボ核酸を抽出するための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013229632A Pending JP2014057590A (ja) | 2006-10-06 | 2013-11-05 | リボ核酸を抽出するための方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US20100099149A1 (https=) |
| EP (1) | EP2066686A4 (https=) |
| JP (2) | JP2010505396A (https=) |
| AU (1) | AU2007304776A1 (https=) |
| CA (1) | CA2664696C (https=) |
| IL (1) | IL197916A (https=) |
| NZ (1) | NZ576003A (https=) |
| SG (1) | SG179454A1 (https=) |
| WO (1) | WO2008040126A1 (https=) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| WO2005113147A2 (en) | 2004-04-08 | 2005-12-01 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| US8221381B2 (en) | 2005-12-09 | 2012-07-17 | Dna Genotek Inc. | Container system for releasably storing a substance |
| AU2007304776A1 (en) | 2006-10-06 | 2008-04-10 | Dna Genotek Inc. | Stabilizing compositions and methods for extraction of ribonucleic acid |
| US8975026B2 (en) | 2007-01-16 | 2015-03-10 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| DE102007025275A1 (de) * | 2007-05-31 | 2008-12-04 | Qiagen Gmbh | Butendisäure oder deren Derivate zur Behandlung einer biologischen Probe |
| WO2009142743A1 (en) * | 2008-05-20 | 2009-11-26 | Cantimer, Inc. | Methods, systems and devices for analyzing a surfactant-treated biological fluid sample |
| US8703416B2 (en) * | 2008-07-17 | 2014-04-22 | Somalogic, Inc. | Method for purification and identification of sperm cells |
| EP3206009B1 (en) | 2008-08-21 | 2025-05-14 | DNA Genotek Inc. | Sample receiving device |
| WO2010083844A1 (en) | 2009-01-26 | 2010-07-29 | Quantibact A/S | Methods and uses for rna extract and storage |
| WO2012018639A2 (en) * | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
| EP2598660B1 (en) | 2010-07-26 | 2017-03-15 | Biomatrica, INC. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| WO2012021493A2 (en) | 2010-08-13 | 2012-02-16 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| WO2012177656A2 (en) | 2011-06-19 | 2012-12-27 | Abogen, Inc. | Devices, solutions and methods for sample collection |
| US9518901B2 (en) | 2011-06-29 | 2016-12-13 | Kabushiki Kaisha Dnaform | Pretreatment method of biological sample, detection method of RNA, and pretreatment kit |
| EP3789500A1 (en) | 2011-08-12 | 2021-03-10 | QIAGEN GmbH | Method for isolating nucleic acids |
| RU2601129C2 (ru) | 2012-04-09 | 2016-10-27 | Инвайролоджикс Инк. | Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце |
| US9725703B2 (en) | 2012-12-20 | 2017-08-08 | Biomatrica, Inc. | Formulations and methods for stabilizing PCR reagents |
| JP5904153B2 (ja) * | 2013-03-29 | 2016-04-13 | ソニー株式会社 | 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ |
| WO2015017701A1 (en) | 2013-08-01 | 2015-02-05 | Ancestry.Com Dna, Llc | Sample collection device |
| WO2015031994A1 (en) * | 2013-09-03 | 2015-03-12 | Dna Genotek Inc. | Method and composition for nucleic acid storage from blood fractions |
| KR102392407B1 (ko) * | 2014-03-07 | 2022-04-29 | 디엔에이 제노텍 인코퍼레이티드 | 생물학적 샘플에서 핵산을 안정화하기 위한 조성물 및 방법 |
| EP3748011B1 (en) | 2014-04-22 | 2024-11-13 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting dna amplification |
| JP2017516477A (ja) * | 2014-05-27 | 2017-06-22 | ディーエヌエー ジェノテック インク | 頑丈な微生物の生存能力を安定化および維持するための組成物および方法 |
| ES2786373T3 (es) | 2014-06-10 | 2020-10-09 | Biomatrica Inc | Estabilización de trombocitos a temperaturas ambiente |
| JP6435139B2 (ja) * | 2014-09-02 | 2018-12-05 | 国立大学法人 鹿児島大学 | 染色体キメラの検出方法 |
| CN107208137A (zh) * | 2014-10-20 | 2017-09-26 | 龙公司 | 检测rna病毒的组合物和方法 |
| US9896682B2 (en) | 2015-03-06 | 2018-02-20 | Bio-Rad Laboratories, Inc. | Stabilized RNA solutions |
| ES2818569T3 (es) | 2015-09-09 | 2021-04-13 | Drawbridge Health Inc | Métodos para la recopilación, estabilización y conservación de muestras |
| SG11201803410RA (en) * | 2015-10-30 | 2018-05-30 | Bonac Corp | COMPOSITION STABLY CONTAINING SINGLE-STRANDED NUCLEIC ACID MOLECULE THAT SUPPRESSES EXPRESSION OF TGF-ß1 GENE |
| US10905113B2 (en) | 2015-11-12 | 2021-02-02 | Regents Of The University Of Minnesota | Compositions and method for storing liquid biospecimens |
| KR20250047404A (ko) | 2015-12-08 | 2025-04-03 | 바이오매트리카 인코포레이티드 | 적혈구 침강 속도의 감소 |
| JP6733951B2 (ja) * | 2016-05-09 | 2020-08-05 | 日本メナード化粧品株式会社 | 核酸保存用組成物及び核酸保存方法 |
| EP3464589A4 (en) | 2016-05-31 | 2020-02-26 | DNA Genotek Inc. | A composition, system and method for removal of detergents from aqueous solutions |
| WO2018170186A1 (en) | 2017-03-15 | 2018-09-20 | Ancestry.Com Dna, Llc | Sample collection device and method |
| NZ764035A (en) | 2017-10-06 | 2020-05-29 | Ancestry Com Dna Llc | Systems, devices, and methods for sample collection |
| US11426734B2 (en) | 2017-11-22 | 2022-08-30 | Ancestry.Com Dna, Llc | Sample collection kit including cap having selectively movable sleeve |
| MX2020005246A (es) | 2017-11-22 | 2020-11-09 | Ancestry Com Dna Llc | Kit de recolección de muestras que incluye tapa con manguito que se puede mover de manera selectiva. |
| LT3752836T (lt) | 2018-02-14 | 2023-02-10 | Salignostics Ltd. | Sudėtinių dalių aptikimo būdai ir aparatas |
| CA3120086A1 (en) * | 2018-11-14 | 2020-05-22 | Spectrum Solutions L.L.C. | Rna preservation solution and methods of manufacture and use |
| US11712692B2 (en) | 2018-11-20 | 2023-08-01 | Spectrum Solutions L.L.C. | Sample collection system including sealing cap and valve |
| AU2020227306A1 (en) | 2019-02-27 | 2021-10-07 | Ancestry.Com Dna, Llc | Graphical user interface displaying relatedness based on shared DNA |
| US11968975B2 (en) | 2019-04-30 | 2024-04-30 | Regents Of The University Of Minnesota | Compositions and methods for storing liquid biospecimens |
| US11701094B2 (en) | 2019-06-20 | 2023-07-18 | Spectrum Solutions L.L.C. | Sample collection system including valve and plug assemblies |
| EP4120907A4 (en) * | 2020-03-16 | 2024-05-08 | Seegene, Inc. | SAMPLING KIT USED IN DETERMINATION OF RESPIRATORY INFECTION |
| KR102370468B1 (ko) * | 2020-03-18 | 2022-03-03 | 재단법인대구경북과학기술원 | 생체 시료 내 잔존하는 음이온 계면 활성제와 이와 결합한 지질 또는 단백질 결합체, 및 마이셀을 제거하는 조성물, 방법 및 이의 용도 |
| EP3996732A4 (en) * | 2020-04-14 | 2022-08-24 | Spectrum Solutions, LLC | ASSAYS AND METHODS FOR DETECTION OF VIRAL NUCLEIC ACIDS |
| US11926877B2 (en) | 2020-06-18 | 2024-03-12 | The Board Of Trustees Of The University Of Illinois | Saliva-based molecular testing for SARS-CoV-2 |
| WO2021044064A1 (de) * | 2020-06-22 | 2021-03-11 | Lead Horizon Gmbh | Vorrichtung zur gewinnung einer rachenspülprobe |
| AT17544U1 (de) * | 2020-06-22 | 2022-07-15 | Lead Horizon Gmbh | Vorrichtung zur gewinnung einer rachenspülprobe |
| WO2022015514A1 (en) * | 2020-07-13 | 2022-01-20 | The Board Of Trustees Of The Leland Stanford Junior University | Systems and methods to enhance rna stability and translation and uses thereof |
| US11207057B1 (en) | 2020-08-07 | 2021-12-28 | Cyrano Medical, Inc. | Nasopharyngeal sample collection devices and methods |
| US11724077B2 (en) * | 2021-07-28 | 2023-08-15 | Subhash Dhawan | Therapeutic swabs for treating upper respiratory infections |
| US12332902B2 (en) | 2022-04-20 | 2025-06-17 | Ancestry.Com Dna, Llc | Filtering individual datasets in a database |
| WO2024189656A1 (en) * | 2023-03-16 | 2024-09-19 | Pt. Jawahar Lal Nehru Memorial Medical College | An in-vitro method and a kit for determining the severity of covid-19 outcome |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935340A (en) * | 1985-06-07 | 1990-06-19 | Eli Lilly And Company | Method of isolating antibiotic biosynthetic genes |
| US5756126A (en) * | 1991-05-29 | 1998-05-26 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| DE4034036C2 (de) * | 1990-10-26 | 1994-03-03 | Diagen Inst Molekularbio | Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen |
| AU664050B2 (en) * | 1991-12-18 | 1995-11-02 | Becton Dickinson & Company | Process for lysing mycobacteria |
| US5512440A (en) * | 1991-12-18 | 1996-04-30 | Becton Dickinson And Company | Process for lysing Mycobacteria |
| US6551791B1 (en) * | 1995-12-21 | 2003-04-22 | University Of Florida | Rapid diagnostic method for distinguishing allergies and infections and nasal secretion collection unit |
| US5973137A (en) * | 1996-02-13 | 1999-10-26 | Gentra Systems, Inc. | Low pH RNA isolation reagents, method, and kit |
| US5939262A (en) * | 1996-07-03 | 1999-08-17 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
| US5939259A (en) * | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
| US6071745A (en) * | 1997-06-27 | 2000-06-06 | Bio-Rad Laboratories | Method and formulation for lyophilizing cultured human cells to preserve RNA and DNA contained in cells for use in molecular biology experiments |
| US7626017B2 (en) * | 1997-10-31 | 2009-12-01 | Pressure Biosciences, Inc. | Pressure-enhanced extraction and purification |
| US7244828B2 (en) * | 1997-11-07 | 2007-07-17 | Aftab Alam | Agent for protein precipitation, a method of protein precipitation, a method of protein assay using protein precipitation agent, and a kit for protein assay |
| US6204375B1 (en) * | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
| DE19836559A1 (de) * | 1998-08-12 | 2000-03-23 | Antigen Gmbh | Gefäß zur Entnahme von Blut |
| US6777210B1 (en) * | 1998-09-24 | 2004-08-17 | Ambion, Inc. | Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1 |
| DE60044977D1 (de) * | 1999-09-24 | 2010-10-28 | Ambion Inc | Cocktail von nukleaseinhibitoren |
| US6409528B1 (en) * | 1999-12-06 | 2002-06-25 | Becton, Dickinson And Company | Device and method for collecting, preparation and stabilizing a sample |
| US7005266B2 (en) * | 2000-02-04 | 2006-02-28 | Qiagen Gmbh | Nucleic acid isolation from stool samples and other inhibitor-rich biological materials |
| US6602718B1 (en) * | 2000-11-08 | 2003-08-05 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
| US7029840B2 (en) * | 2000-11-15 | 2006-04-18 | Becton, Dickinson And Company | Method for preservation of cells and nucleic acid targets |
| US7001724B1 (en) * | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| US20030170694A1 (en) * | 2001-12-21 | 2003-09-11 | Daniel Wall | Stabilized nucleic acids in gene and drug discovery and methods of use |
| DE10219117C1 (de) * | 2002-04-29 | 2003-10-30 | Adnagen Ag | Verfahren zur Stabilisierung von RNS und Verwendungen von Stabilisierungspuffern |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| JP5441290B2 (ja) * | 2002-10-04 | 2014-03-12 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 核酸精製システムの媒体上に核酸を保存するツールとして、化学物質を用いる方法および材料 |
| US8158357B2 (en) | 2005-03-16 | 2012-04-17 | Dna Genotek Inc. | Compositions and method for storage of nucleic acid from bodily fluids |
| WO2006119292A2 (en) * | 2005-04-29 | 2006-11-09 | Henkin, Robert | Methods for detection of biological substances |
| US20070015165A1 (en) * | 2005-07-13 | 2007-01-18 | Sigma-Aldrich Co. | Method for the isolation of RNA from biological sources |
| US8221381B2 (en) | 2005-12-09 | 2012-07-17 | Dna Genotek Inc. | Container system for releasably storing a substance |
| EP1996070A2 (en) | 2006-03-17 | 2008-12-03 | The Regents of the University of California | Collection assembly for obtaining oral samples from an animal |
| AU2007304776A1 (en) * | 2006-10-06 | 2008-04-10 | Dna Genotek Inc. | Stabilizing compositions and methods for extraction of ribonucleic acid |
-
2007
- 2007-10-05 AU AU2007304776A patent/AU2007304776A1/en not_active Abandoned
- 2007-10-05 NZ NZ576003A patent/NZ576003A/en unknown
- 2007-10-05 WO PCT/CA2007/001785 patent/WO2008040126A1/en not_active Ceased
- 2007-10-05 US US12/444,447 patent/US20100099149A1/en not_active Abandoned
- 2007-10-05 JP JP2009530744A patent/JP2010505396A/ja active Pending
- 2007-10-05 SG SG2012016606A patent/SG179454A1/en unknown
- 2007-10-05 EP EP07855412A patent/EP2066686A4/en not_active Ceased
- 2007-10-05 CA CA2664696A patent/CA2664696C/en active Active
-
2009
- 2009-04-05 IL IL197916A patent/IL197916A/en active IP Right Grant
-
2013
- 2013-11-05 JP JP2013229632A patent/JP2014057590A/ja active Pending
-
2016
- 2016-05-20 US US15/160,712 patent/US10000795B2/en active Active
-
2018
- 2018-05-15 US US15/980,682 patent/US10767215B2/en active Active
-
2020
- 2020-09-08 US US17/014,688 patent/US20210198717A1/en not_active Abandoned
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