JP2016527897A5 - - Google Patents
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- Publication number
- JP2016527897A5 JP2016527897A5 JP2016533928A JP2016533928A JP2016527897A5 JP 2016527897 A5 JP2016527897 A5 JP 2016527897A5 JP 2016533928 A JP2016533928 A JP 2016533928A JP 2016533928 A JP2016533928 A JP 2016533928A JP 2016527897 A5 JP2016527897 A5 JP 2016527897A5
- Authority
- JP
- Japan
- Prior art keywords
- matrix
- acid
- rna
- combinations
- rnase inhibitor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011159 matrix material Substances 0.000 claims 32
- 239000002253 acid Substances 0.000 claims 10
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims 6
- 239000003161 ribonuclease inhibitor Substances 0.000 claims 6
- 229920002477 rna polymer Polymers 0.000 claims 6
- 238000001035 drying Methods 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 239000007787 solid Substances 0.000 claims 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- 238000004448 titration Methods 0.000 claims 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 150000007513 acids Chemical class 0.000 claims 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 3
- 239000003398 denaturant Substances 0.000 claims 3
- 230000036571 hydration Effects 0.000 claims 3
- 238000006703 hydration reaction Methods 0.000 claims 3
- NWVVVBRKAWDGAB-UHFFFAOYSA-N p-methoxyphenol Chemical compound COC1=CC=C(O)C=C1 NWVVVBRKAWDGAB-UHFFFAOYSA-N 0.000 claims 3
- 239000002516 radical scavenger Substances 0.000 claims 3
- CNHDIAIOKMXOLK-UHFFFAOYSA-N toluquinol Chemical compound CC1=CC(O)=CC=C1O CNHDIAIOKMXOLK-UHFFFAOYSA-N 0.000 claims 3
- SRSAMLMFVUJPSR-UHFFFAOYSA-N 2-carboxyethylphosphanium;chloride Chemical compound Cl.OC(=O)CCP SRSAMLMFVUJPSR-UHFFFAOYSA-N 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 238000002123 RNA extraction Methods 0.000 claims 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 2
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims 2
- 239000007983 Tris buffer Substances 0.000 claims 2
- 230000002378 acidificating effect Effects 0.000 claims 2
- 229960005070 ascorbic acid Drugs 0.000 claims 2
- 235000010323 ascorbic acid Nutrition 0.000 claims 2
- 239000011668 ascorbic acid Substances 0.000 claims 2
- 230000003196 chaotropic effect Effects 0.000 claims 2
- 239000003638 chemical reducing agent Substances 0.000 claims 2
- 239000003599 detergent Substances 0.000 claims 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 2
- 235000011180 diphosphates Nutrition 0.000 claims 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical group SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 229940048084 pyrophosphate Drugs 0.000 claims 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims 1
- PBVAJRFEEOIAGW-UHFFFAOYSA-N 3-[bis(2-carboxyethyl)phosphanyl]propanoic acid;hydrochloride Chemical compound Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O PBVAJRFEEOIAGW-UHFFFAOYSA-N 0.000 claims 1
- 239000004475 Arginine Substances 0.000 claims 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims 1
- 239000000020 Nitrocellulose Substances 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims 1
- 230000006750 UV protection Effects 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002301 cellulose acetate Polymers 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 239000003365 glass fiber Substances 0.000 claims 1
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims 1
- 229910017604 nitric acid Inorganic materials 0.000 claims 1
- 229920001220 nitrocellulos Polymers 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- HWGNBUXHKFFFIH-UHFFFAOYSA-I pentasodium;[oxido(phosphonatooxy)phosphoryl] phosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O HWGNBUXHKFFFIH-UHFFFAOYSA-I 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 claims 1
- 229940116357 potassium thiocyanate Drugs 0.000 claims 1
- 239000011814 protection agent Substances 0.000 claims 1
- 239000003223 protective agent Substances 0.000 claims 1
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 claims 1
- 239000002342 ribonucleoside Substances 0.000 claims 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 claims 1
- 229940048086 sodium pyrophosphate Drugs 0.000 claims 1
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 claims 1
- 235000019832 sodium triphosphate Nutrition 0.000 claims 1
- 239000011975 tartaric acid Substances 0.000 claims 1
- 235000002906 tartaric acid Nutrition 0.000 claims 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 1
- -1 vanadyl ribonucleoside Chemical compound 0.000 claims 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 claims 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/968,497 | 2013-08-16 | ||
| US13/968,497 US9040679B2 (en) | 2012-04-30 | 2013-08-16 | Methods and compositions for extraction and storage of nucleic acids |
| PCT/EP2014/067453 WO2015022410A1 (en) | 2013-08-16 | 2014-08-14 | Methods and compositions for extraction and storage of nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016527897A JP2016527897A (ja) | 2016-09-15 |
| JP2016527897A5 true JP2016527897A5 (https=) | 2017-09-21 |
| JP6704621B2 JP6704621B2 (ja) | 2020-06-03 |
Family
ID=51355538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016533928A Active JP6704621B2 (ja) | 2013-08-16 | 2014-08-14 | 核酸の抽出及び保存のための方法及び組成物 |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP3033421B1 (https=) |
| JP (1) | JP6704621B2 (https=) |
| KR (1) | KR102214211B1 (https=) |
| CN (1) | CN105431536B (https=) |
| AU (1) | AU2014307875B2 (https=) |
| CA (1) | CA2921286C (https=) |
| ES (1) | ES2777220T3 (https=) |
| IL (1) | IL244033B (https=) |
| MX (1) | MX371517B (https=) |
| SG (1) | SG11201601114PA (https=) |
| WO (1) | WO2015022410A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2903175T3 (es) | 2015-03-31 | 2022-03-31 | Hoffmann La Roche | Tarjeta de separación de plasma |
| CN108473932B (zh) | 2015-09-09 | 2022-07-15 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
| WO2018081161A1 (en) * | 2016-10-24 | 2018-05-03 | Biomatrica, Inc. | Stabilization of nucleic acids on paper |
| CN106754881A (zh) * | 2016-12-26 | 2017-05-31 | 广州和实生物技术有限公司 | 一种血液病毒rna保护剂及其制备方法与应用 |
| TWI882519B (zh) | 2017-01-10 | 2025-05-01 | 美商集聯健康有限公司 | 用於收集和儲存來自受試者之血液的裝置 |
| CN109182332A (zh) * | 2018-09-30 | 2019-01-11 | 浙江天杭生物科技股份有限公司 | 一种从临床样品中快速简便提取核酸的方法 |
| EP4206323A4 (en) * | 2020-08-28 | 2024-08-28 | Kao Corporation | Rna storage method |
| EP4387842A4 (en) * | 2021-08-16 | 2025-07-16 | Millimeter Wave Products Inc Tlc | SAMPLE ENCAPSULATION SYSTEM |
| CN117467657A (zh) * | 2022-07-22 | 2024-01-30 | 友康厚德生物科技(北京)有限公司 | 一种裂解组合物 |
| CN120665858A (zh) * | 2025-06-17 | 2025-09-19 | 郑州安图生物工程股份有限公司 | 样本处理试剂、核酸提取试剂以及病原体核酸的提取方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996039813A1 (en) * | 1995-06-07 | 1996-12-19 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| US7267950B2 (en) * | 2003-05-01 | 2007-09-11 | Veridex, Lcc | Rapid extraction of RNA from cells and tissues |
| US20070015165A1 (en) * | 2005-07-13 | 2007-01-18 | Sigma-Aldrich Co. | Method for the isolation of RNA from biological sources |
| KR100738086B1 (ko) * | 2005-12-21 | 2007-07-12 | 삼성전자주식회사 | 금속 산화물이 증착된 고체 지지체를 이용한 핵산 정제방법 및 장치 |
| US20090053704A1 (en) * | 2007-08-24 | 2009-02-26 | Natalia Novoradovskaya | Stabilization of nucleic acids on solid supports |
| EP2334792B1 (en) * | 2008-09-12 | 2020-09-09 | GenTegra LLC | Matrices and media for storage and stabilization of biomolecules |
| EP2430195B1 (en) * | 2009-05-11 | 2019-01-23 | Biomatrica, INC. | Compositions and methods for biological sample storage |
| LT2521780T (lt) * | 2010-01-07 | 2018-02-12 | Bigtec Private Limited | Neklerūgščių išskyrimo būdas ir rinkinys |
| CA2806670A1 (en) * | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| CN103068979B (zh) * | 2010-08-18 | 2015-03-11 | 东丽株式会社 | Rna提取用溶液 |
| US9044738B2 (en) * | 2012-04-30 | 2015-06-02 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
-
2014
- 2014-08-14 WO PCT/EP2014/067453 patent/WO2015022410A1/en not_active Ceased
- 2014-08-14 CA CA2921286A patent/CA2921286C/en active Active
- 2014-08-14 JP JP2016533928A patent/JP6704621B2/ja active Active
- 2014-08-14 MX MX2016001843A patent/MX371517B/es active IP Right Grant
- 2014-08-14 ES ES14752322T patent/ES2777220T3/es active Active
- 2014-08-14 SG SG11201601114PA patent/SG11201601114PA/en unknown
- 2014-08-14 CN CN201480045324.5A patent/CN105431536B/zh active Active
- 2014-08-14 AU AU2014307875A patent/AU2014307875B2/en active Active
- 2014-08-14 KR KR1020167003478A patent/KR102214211B1/ko active Active
- 2014-08-14 EP EP14752322.9A patent/EP3033421B1/en active Active
-
2016
- 2016-02-09 IL IL244033A patent/IL244033B/en active IP Right Grant
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