CA2921286C - Methods and compositions for extraction and storage of nucleic acids - Google Patents

Methods and compositions for extraction and storage of nucleic acids Download PDF

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Publication number
CA2921286C
CA2921286C CA2921286A CA2921286A CA2921286C CA 2921286 C CA2921286 C CA 2921286C CA 2921286 A CA2921286 A CA 2921286A CA 2921286 A CA2921286 A CA 2921286A CA 2921286 C CA2921286 C CA 2921286C
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CA
Canada
Prior art keywords
matrix
acid
nucleic acids
rna
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2921286A
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English (en)
French (fr)
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CA2921286A1 (en
Inventor
Erik Leeming Kvam
Bing Li
Brian Christopher Bales
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions Operations UK Ltd
Original Assignee
Global Life Sciences Solutions Operations UK Ltd
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Filing date
Publication date
Priority claimed from US13/968,497 external-priority patent/US9040679B2/en
Application filed by Global Life Sciences Solutions Operations UK Ltd filed Critical Global Life Sciences Solutions Operations UK Ltd
Publication of CA2921286A1 publication Critical patent/CA2921286A1/en
Application granted granted Critical
Publication of CA2921286C publication Critical patent/CA2921286C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2921286A 2013-08-16 2014-08-14 Methods and compositions for extraction and storage of nucleic acids Active CA2921286C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/968,497 2013-08-16
US13/968,497 US9040679B2 (en) 2012-04-30 2013-08-16 Methods and compositions for extraction and storage of nucleic acids
PCT/EP2014/067453 WO2015022410A1 (en) 2013-08-16 2014-08-14 Methods and compositions for extraction and storage of nucleic acids

Publications (2)

Publication Number Publication Date
CA2921286A1 CA2921286A1 (en) 2015-02-19
CA2921286C true CA2921286C (en) 2022-11-22

Family

ID=51355538

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2921286A Active CA2921286C (en) 2013-08-16 2014-08-14 Methods and compositions for extraction and storage of nucleic acids

Country Status (11)

Country Link
EP (1) EP3033421B1 (https=)
JP (1) JP6704621B2 (https=)
KR (1) KR102214211B1 (https=)
CN (1) CN105431536B (https=)
AU (1) AU2014307875B2 (https=)
CA (1) CA2921286C (https=)
ES (1) ES2777220T3 (https=)
IL (1) IL244033B (https=)
MX (1) MX371517B (https=)
SG (1) SG11201601114PA (https=)
WO (1) WO2015022410A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2903175T3 (es) 2015-03-31 2022-03-31 Hoffmann La Roche Tarjeta de separación de plasma
CN108473932B (zh) 2015-09-09 2022-07-15 集联健康有限公司 用于样品收集、稳定化和保存的系统、方法和装置
WO2018081161A1 (en) * 2016-10-24 2018-05-03 Biomatrica, Inc. Stabilization of nucleic acids on paper
CN106754881A (zh) * 2016-12-26 2017-05-31 广州和实生物技术有限公司 一种血液病毒rna保护剂及其制备方法与应用
TWI882519B (zh) 2017-01-10 2025-05-01 美商集聯健康有限公司 用於收集和儲存來自受試者之血液的裝置
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
EP4206323A4 (en) * 2020-08-28 2024-08-28 Kao Corporation Rna storage method
EP4387842A4 (en) * 2021-08-16 2025-07-16 Millimeter Wave Products Inc Tlc SAMPLE ENCAPSULATION SYSTEM
CN117467657A (zh) * 2022-07-22 2024-01-30 友康厚德生物科技(北京)有限公司 一种裂解组合物
CN120665858A (zh) * 2025-06-17 2025-09-19 郑州安图生物工程股份有限公司 样本处理试剂、核酸提取试剂以及病原体核酸的提取方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996039813A1 (en) * 1995-06-07 1996-12-19 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
KR100738086B1 (ko) * 2005-12-21 2007-07-12 삼성전자주식회사 금속 산화물이 증착된 고체 지지체를 이용한 핵산 정제방법 및 장치
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
EP2334792B1 (en) * 2008-09-12 2020-09-09 GenTegra LLC Matrices and media for storage and stabilization of biomolecules
EP2430195B1 (en) * 2009-05-11 2019-01-23 Biomatrica, INC. Compositions and methods for biological sample storage
LT2521780T (lt) * 2010-01-07 2018-02-12 Bigtec Private Limited Neklerūgščių išskyrimo būdas ir rinkinys
CA2806670A1 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
CN103068979B (zh) * 2010-08-18 2015-03-11 东丽株式会社 Rna提取用溶液
US9044738B2 (en) * 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids

Also Published As

Publication number Publication date
KR20160034947A (ko) 2016-03-30
IL244033A0 (en) 2016-04-21
AU2014307875B2 (en) 2020-07-02
CN105431536A (zh) 2016-03-23
JP6704621B2 (ja) 2020-06-03
EP3033421B1 (en) 2020-02-12
EP3033421A1 (en) 2016-06-22
MX2016001843A (es) 2016-05-16
CA2921286A1 (en) 2015-02-19
WO2015022410A1 (en) 2015-02-19
ES2777220T3 (es) 2020-08-04
CN105431536B (zh) 2021-01-01
SG11201601114PA (en) 2016-03-30
AU2014307875A1 (en) 2016-01-28
KR102214211B1 (ko) 2021-02-10
IL244033B (en) 2020-07-30
MX371517B (es) 2020-01-31
JP2016527897A (ja) 2016-09-15

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