CN105431536B - 用于提取和储存核酸的方法和组合物 - Google Patents

用于提取和储存核酸的方法和组合物 Download PDF

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Publication number
CN105431536B
CN105431536B CN201480045324.5A CN201480045324A CN105431536B CN 105431536 B CN105431536 B CN 105431536B CN 201480045324 A CN201480045324 A CN 201480045324A CN 105431536 B CN105431536 B CN 105431536B
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matrix
acid
rna
nucleic acids
sample
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Chinese (zh)
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CN105431536A (zh
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E.L.克瓦姆
B.李
B.C.贝尔斯
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GE Healthcare UK Ltd
Global Life Sciences Solutions Operations UK Ltd
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General Electric Co
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Priority claimed from US13/968,497 external-priority patent/US9040679B2/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480045324.5A 2013-08-16 2014-08-14 用于提取和储存核酸的方法和组合物 Active CN105431536B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/968497 2013-08-16
US13/968,497 US9040679B2 (en) 2012-04-30 2013-08-16 Methods and compositions for extraction and storage of nucleic acids
PCT/EP2014/067453 WO2015022410A1 (en) 2013-08-16 2014-08-14 Methods and compositions for extraction and storage of nucleic acids

Publications (2)

Publication Number Publication Date
CN105431536A CN105431536A (zh) 2016-03-23
CN105431536B true CN105431536B (zh) 2021-01-01

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CN201480045324.5A Active CN105431536B (zh) 2013-08-16 2014-08-14 用于提取和储存核酸的方法和组合物

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EP (1) EP3033421B1 (https=)
JP (1) JP6704621B2 (https=)
KR (1) KR102214211B1 (https=)
CN (1) CN105431536B (https=)
AU (1) AU2014307875B2 (https=)
CA (1) CA2921286C (https=)
ES (1) ES2777220T3 (https=)
IL (1) IL244033B (https=)
MX (1) MX371517B (https=)
SG (1) SG11201601114PA (https=)
WO (1) WO2015022410A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2903175T3 (es) 2015-03-31 2022-03-31 Hoffmann La Roche Tarjeta de separación de plasma
CN108473932B (zh) 2015-09-09 2022-07-15 集联健康有限公司 用于样品收集、稳定化和保存的系统、方法和装置
WO2018081161A1 (en) * 2016-10-24 2018-05-03 Biomatrica, Inc. Stabilization of nucleic acids on paper
CN106754881A (zh) * 2016-12-26 2017-05-31 广州和实生物技术有限公司 一种血液病毒rna保护剂及其制备方法与应用
TWI882519B (zh) 2017-01-10 2025-05-01 美商集聯健康有限公司 用於收集和儲存來自受試者之血液的裝置
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
EP4206323A4 (en) * 2020-08-28 2024-08-28 Kao Corporation Rna storage method
EP4387842A4 (en) * 2021-08-16 2025-07-16 Millimeter Wave Products Inc Tlc SAMPLE ENCAPSULATION SYSTEM
CN117467657A (zh) * 2022-07-22 2024-01-30 友康厚德生物科技(北京)有限公司 一种裂解组合物
CN120665858A (zh) * 2025-06-17 2025-09-19 郑州安图生物工程股份有限公司 样本处理试剂、核酸提取试剂以及病原体核酸的提取方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996039813A1 (en) * 1995-06-07 1996-12-19 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
KR100738086B1 (ko) * 2005-12-21 2007-07-12 삼성전자주식회사 금속 산화물이 증착된 고체 지지체를 이용한 핵산 정제방법 및 장치
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
EP2334792B1 (en) * 2008-09-12 2020-09-09 GenTegra LLC Matrices and media for storage and stabilization of biomolecules
EP2430195B1 (en) * 2009-05-11 2019-01-23 Biomatrica, INC. Compositions and methods for biological sample storage
LT2521780T (lt) * 2010-01-07 2018-02-12 Bigtec Private Limited Neklerūgščių išskyrimo būdas ir rinkinys
CA2806670A1 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
CN103068979B (zh) * 2010-08-18 2015-03-11 东丽株式会社 Rna提取用溶液
US9044738B2 (en) * 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids

Also Published As

Publication number Publication date
KR20160034947A (ko) 2016-03-30
IL244033A0 (en) 2016-04-21
AU2014307875B2 (en) 2020-07-02
CN105431536A (zh) 2016-03-23
JP6704621B2 (ja) 2020-06-03
EP3033421B1 (en) 2020-02-12
EP3033421A1 (en) 2016-06-22
MX2016001843A (es) 2016-05-16
CA2921286A1 (en) 2015-02-19
WO2015022410A1 (en) 2015-02-19
ES2777220T3 (es) 2020-08-04
SG11201601114PA (en) 2016-03-30
AU2014307875A1 (en) 2016-01-28
KR102214211B1 (ko) 2021-02-10
IL244033B (en) 2020-07-30
MX371517B (es) 2020-01-31
CA2921286C (en) 2022-11-22
JP2016527897A (ja) 2016-09-15

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Effective date of registration: 20210825

Address after: England

Patentee after: Global life science solutions UK Ltd.

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Patentee before: GE HEALTHCARE UK Ltd.

Effective date of registration: 20210825

Address after: Buckinghamshire

Patentee after: GE HEALTHCARE UK Ltd.

Address before: New York State, USA

Patentee before: General Electric Co.