JP2009521954A5 - - Google Patents
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- Publication number
- JP2009521954A5 JP2009521954A5 JP2008549582A JP2008549582A JP2009521954A5 JP 2009521954 A5 JP2009521954 A5 JP 2009521954A5 JP 2008549582 A JP2008549582 A JP 2008549582A JP 2008549582 A JP2008549582 A JP 2008549582A JP 2009521954 A5 JP2009521954 A5 JP 2009521954A5
- Authority
- JP
- Japan
- Prior art keywords
- composition according
- dna polymerase
- composition
- range
- units
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 claims 19
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 7
- 229910017053 inorganic salt Inorganic materials 0.000 claims 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims 4
- 239000007853 buffer solution Substances 0.000 claims 3
- 239000012141 concentrate Substances 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- NLSCHDZTHVNDCP-UHFFFAOYSA-N caesium nitrate Chemical compound [Cs+].[O-][N+]([O-])=O NLSCHDZTHVNDCP-UHFFFAOYSA-N 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- 238000002703 mutagenesis Methods 0.000 claims 1
- 231100000350 mutagenesis Toxicity 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000005022 packaging material Substances 0.000 claims 1
- 238000006116 polymerization reaction Methods 0.000 claims 1
- 239000001103 potassium chloride Substances 0.000 claims 1
- 235000011164 potassium chloride Nutrition 0.000 claims 1
- 235000010333 potassium nitrate Nutrition 0.000 claims 1
- 239000004323 potassium nitrate Substances 0.000 claims 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 1
- 229910052939 potassium sulfate Inorganic materials 0.000 claims 1
- 235000011151 potassium sulphates Nutrition 0.000 claims 1
- 230000010076 replication Effects 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75712006P | 2006-01-06 | 2006-01-06 | |
| US60/757,120 | 2006-01-06 | ||
| PCT/US2007/000352 WO2007081841A2 (en) | 2006-01-06 | 2007-01-08 | Reaction buffer composition for nucleic acid replication with packed dna polymerases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009521954A JP2009521954A (ja) | 2009-06-11 |
| JP2009521954A5 true JP2009521954A5 (enExample) | 2010-02-25 |
| JP5106416B2 JP5106416B2 (ja) | 2012-12-26 |
Family
ID=38256944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008549582A Expired - Fee Related JP5106416B2 (ja) | 2006-01-06 | 2007-01-08 | 濃縮（ｐａｃｋｅｄ）ＤＮＡポリメラーゼを含む核酸複製のための反応緩衝液組成物 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7939645B2 (enExample) |
| EP (1) | EP1989318B1 (enExample) |
| JP (1) | JP5106416B2 (enExample) |
| WO (1) | WO2007081841A2 (enExample) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8283148B2 (en) * | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
| AU2004252568B2 (en) | 2003-06-27 | 2011-06-30 | Ethicon, Incorporated | Regeneration and repair of neural tissue using postpartum-derived cells |
| US9572840B2 (en) | 2003-06-27 | 2017-02-21 | DePuy Synthes Products, Inc. | Regeneration and repair of neural tissue using postpartum-derived cells |
| US8790637B2 (en) | 2003-06-27 | 2014-07-29 | DePuy Synthes Products, LLC | Repair and regeneration of ocular tissue using postpartum-derived cells |
| AU2006202209B2 (en) * | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| CN114214394A (zh) * | 2012-06-08 | 2022-03-22 | 爱奥尼安技术公司 | 核苷酸扩增反应 |
| SG10202009015SA (en) | 2012-12-10 | 2020-10-29 | Resolution Bioscience Inc | Methods for targeted genomic analysis |
| KR102122632B1 (ko) | 2013-08-05 | 2020-06-16 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| US9587268B2 (en) | 2014-01-29 | 2017-03-07 | Agilent Technologies Inc. | Fast hybridization for next generation sequencing target enrichment |
| US20160053301A1 (en) | 2014-08-22 | 2016-02-25 | Clearfork Bioscience, Inc. | Methods for quantitative genetic analysis of cell free dna |
| WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| CA3253836A1 (en) | 2015-02-04 | 2025-12-01 | Twist Bioscience Corporation | Compositions and methods for synthetic gene assembly |
| WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| EP3350314A4 (en) | 2015-09-18 | 2019-02-06 | Twist Bioscience Corporation | OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| CA3004435A1 (en) | 2015-11-11 | 2017-05-18 | Resolution Bioscience, Inc. | High efficiency construction of dna libraries |
| CN108603307A (zh) | 2015-12-01 | 2018-09-28 | 特韦斯特生物科学公司 | 功能化表面及其制备 |
| WO2017162518A1 (en) * | 2016-03-19 | 2017-09-28 | Qiagen Gmbh | Stabilization of rna |
| GB2568444A (en) | 2016-08-22 | 2019-05-15 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
| JP2018029529A (ja) * | 2016-08-25 | 2018-03-01 | 東洋紡株式会社 | 標的核酸の検出法 |
| US11319594B2 (en) | 2016-08-25 | 2022-05-03 | Resolution Bioscience, Inc. | Methods for the detection of genomic copy changes in DNA samples |
| JP2018033324A (ja) * | 2016-08-29 | 2018-03-08 | 東洋紡株式会社 | 標的核酸の検出法 |
| US10417457B2 (en) | 2016-09-21 | 2019-09-17 | Twist Bioscience Corporation | Nucleic acid based data storage |
| CN110366613A (zh) | 2016-12-16 | 2019-10-22 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| SG11201907713WA (en) | 2017-02-22 | 2019-09-27 | Twist Bioscience Corp | Nucleic acid based data storage |
| CN110913865A (zh) | 2017-03-15 | 2020-03-24 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| KR102784398B1 (ko) | 2017-06-12 | 2025-03-19 | 트위스트 바이오사이언스 코포레이션 | 심리스 핵산 어셈블리를 위한 방법 |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| JP2020536504A (ja) | 2017-09-11 | 2020-12-17 | ツイスト バイオサイエンス コーポレーション | Ｇｐｃｒ結合タンパク質およびその合成 |
| KR102889470B1 (ko) | 2017-10-20 | 2025-11-21 | 트위스트 바이오사이언스 코포레이션 | 폴리뉴클레오타이드 합성을 위한 가열된 나노웰 |
| CN109988817B (zh) * | 2017-12-30 | 2020-12-04 | 安诺优达基因科技(北京)有限公司 | 一种随机打断dna的方法 |
| WO2019136175A1 (en) | 2018-01-04 | 2019-07-11 | Twist Bioscience Corporation | Dna-based digital information storage |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| CN119708086A (zh) | 2018-12-26 | 2025-03-28 | 特韦斯特生物科学公司 | 高度准确的从头多核苷酸合成 |
| AU2020227802A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| EP3930753A4 (en) | 2019-02-26 | 2023-03-29 | Twist Bioscience Corporation | VARIANT NUCLEIC ACID LIBRARIES FOR GLP1 RECEPTOR |
| CN114729342A (zh) | 2019-06-21 | 2022-07-08 | 特韦斯特生物科学公司 | 基于条形码的核酸序列装配 |
| JP7779828B2 (ja) * | 2019-07-26 | 2025-12-03 | ベクトン・ディキンソン・アンド・カンパニー | 凝集を低減するための緩衝剤組成物 |
| WO2021061829A1 (en) | 2019-09-23 | 2021-04-01 | Twist Bioscience Corporation | Variant nucleic acid libraries for crth2 |
| AU2020355027A1 (en) | 2019-09-23 | 2022-04-21 | Twist Bioscience Corporation | Antibodies that bind CD3 Epsilon |
| CN111876473A (zh) * | 2020-08-07 | 2020-11-03 | 福州大学 | 一种适用于荧光定量pcr中tth dna酶扩增的缓冲液 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5075216A (en) | 1988-09-23 | 1991-12-24 | Cetus Corporation | Methods for dna sequencing with thermus aquaticus dna polymerase |
| US5091310A (en) | 1988-09-23 | 1992-02-25 | Cetus Corporation | Structure-independent dna amplification by the polymerase chain reaction |
| US5066584A (en) | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
| US5023171A (en) | 1989-08-10 | 1991-06-11 | Mayo Foundation For Medical Education And Research | Method for gene splicing by overlap extension using the polymerase chain reaction |
| US5104792A (en) | 1989-12-21 | 1992-04-14 | The United States Of America As Represented By The Department Of Health And Human Services | Method for amplifying unknown nucleic acid sequences |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5972603A (en) * | 1996-02-09 | 1999-10-26 | President And Fellows Of Harvard College | DNA polymerase with modified processivity |
| US6183997B1 (en) | 1997-03-21 | 2001-02-06 | Stratagene | Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same |
| AU7100198A (en) * | 1997-04-03 | 1998-10-22 | Life Technologies, Inc. | Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr) |
| EP0997530B1 (en) | 1997-06-26 | 2006-08-23 | Takara Bio Inc. | Dna polymerase-related factors |
| JP2003506048A (ja) | 1999-07-30 | 2003-02-18 | ストラタジーン | 古細菌複製補助因子及び使用方法 |
| US6300073B1 (en) * | 1999-10-01 | 2001-10-09 | Clontech Laboratories, Inc. | One step RT-PCR methods, enzyme mixes and kits for use in practicing the same |
| US20030228616A1 (en) * | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| US6627424B1 (en) * | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| EP1301205A1 (en) | 2000-07-12 | 2003-04-16 | Invitrogen Corporation | High fidelity polymerases and uses thereof |
| US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
| WO2002097078A1 (en) * | 2001-05-30 | 2002-12-05 | Stratagene | Compositions and methods for random nucleic acid mutagenisis |
| US6403341B1 (en) * | 2001-08-02 | 2002-06-11 | Wayne M. Barnes | Magnesium precipitate hot start method for PCR |
| US6949365B2 (en) * | 2002-06-12 | 2005-09-27 | University Of New Hampshire | Polynucleotides encoding lamprey GnRH-III |
| US7704712B2 (en) | 2003-03-25 | 2010-04-27 | Stratagene California | DNA polymerase fusions and uses thereof |
| US20040214194A1 (en) | 2003-04-17 | 2004-10-28 | Mj Bioworks, Inc. | Methods of making hybrid proteins |
| FR2878254B1 (fr) * | 2004-11-22 | 2010-08-20 | Bio Rad Pasteur | Composition pour l'amplification d'acides nucleiques |
-
2007
- 2007-01-08 EP EP07717819.2A patent/EP1989318B1/en not_active Not-in-force
- 2007-01-08 WO PCT/US2007/000352 patent/WO2007081841A2/en not_active Ceased
- 2007-01-08 JP JP2008549582A patent/JP5106416B2/ja not_active Expired - Fee Related
- 2007-01-08 US US11/651,169 patent/US7939645B2/en active Active
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