JP2010503413A5 - - Google Patents
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- Publication number
- JP2010503413A5 JP2010503413A5 JP2009528513A JP2009528513A JP2010503413A5 JP 2010503413 A5 JP2010503413 A5 JP 2010503413A5 JP 2009528513 A JP2009528513 A JP 2009528513A JP 2009528513 A JP2009528513 A JP 2009528513A JP 2010503413 A5 JP2010503413 A5 JP 2010503413A5
- Authority
- JP
- Japan
- Prior art keywords
- polymerase
- soil
- resistant
- pcr
- assay mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 14
- 239000002689 soil Substances 0.000 claims 11
- 239000000203 mixture Substances 0.000 claims 10
- 239000000975 dye Substances 0.000 claims 7
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- 238000003752 polymerase chain reaction Methods 0.000 claims 6
- 229920001184 polypeptide Polymers 0.000 claims 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 5
- 238000002944 PCR assay Methods 0.000 claims 5
- 238000003556 assay Methods 0.000 claims 5
- 239000008280 blood Substances 0.000 claims 5
- 210000004369 blood Anatomy 0.000 claims 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims 4
- 239000004021 humic acid Substances 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 3
- 239000012503 blood component Substances 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 238000003753 real-time PCR Methods 0.000 claims 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims 1
- -1 PICO Chemical compound 0.000 claims 1
- MZZINWWGSYUHGU-UHFFFAOYSA-J ToTo-1 Chemical compound [I-].[I-].[I-].[I-].C12=CC=CC=C2C(C=C2N(C3=CC=CC=C3S2)C)=CC=[N+]1CCC[N+](C)(C)CCC[N+](C)(C)CCC[N+](C1=CC=CC=C11)=CC=C1C=C1N(C)C2=CC=CC=C2S1 MZZINWWGSYUHGU-UHFFFAOYSA-J 0.000 claims 1
- GRRMZXFOOGQMFA-UHFFFAOYSA-J YoYo-1 Chemical compound [I-].[I-].[I-].[I-].C12=CC=CC=C2C(C=C2N(C3=CC=CC=C3O2)C)=CC=[N+]1CCC[N+](C)(C)CCC[N+](C)(C)CCC[N+](C1=CC=CC=C11)=CC=C1C=C1N(C)C2=CC=CC=C2O1 GRRMZXFOOGQMFA-UHFFFAOYSA-J 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 229960003237 betaine Drugs 0.000 claims 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 claims 1
- 229960005542 ethidium bromide Drugs 0.000 claims 1
- 239000000049 pigment Substances 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82569206P | 2006-09-14 | 2006-09-14 | |
| US60/825,692 | 2006-09-14 | ||
| PCT/US2007/078571 WO2008034110A2 (en) | 2006-09-14 | 2007-09-14 | Use of taq polymerase mutant enzymes for dna amplification in the presence of pcr inhibitors |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013209506A Division JP2014054254A (ja) | 2006-09-14 | 2013-10-04 | Ｐｃｒ阻害物質の存在下におけるｄｎａ増幅のためのｔａｑポリメラーゼ変異体酵素の使用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010503413A JP2010503413A (ja) | 2010-02-04 |
| JP2010503413A5 true JP2010503413A5 (enExample) | 2010-11-04 |
| JP5457184B2 JP5457184B2 (ja) | 2014-04-02 |
Family
ID=39184635
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009528513A Expired - Fee Related JP5457184B2 (ja) | 2006-09-14 | 2007-09-14 | Ｐｃｒ阻害物質の存在下におけるｄｎａ増幅のためのｔａｑポリメラーゼ変異体酵素の使用 |
| JP2013209506A Pending JP2014054254A (ja) | 2006-09-14 | 2013-10-04 | Ｐｃｒ阻害物質の存在下におけるｄｎａ増幅のためのｔａｑポリメラーゼ変異体酵素の使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013209506A Pending JP2014054254A (ja) | 2006-09-14 | 2013-10-04 | Ｐｃｒ阻害物質の存在下におけるｄｎａ増幅のためのｔａｑポリメラーゼ変異体酵素の使用 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20110027832A1 (enExample) |
| EP (1) | EP2061894A4 (enExample) |
| JP (2) | JP5457184B2 (enExample) |
| AU (1) | AU2007296180A1 (enExample) |
| CA (1) | CA2664043A1 (enExample) |
| WO (1) | WO2008034110A2 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7462475B2 (en) | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| EP2076592B1 (en) | 2006-10-23 | 2017-04-12 | Medical Research Council | Polymerase |
| JP2011097828A (ja) * | 2008-07-09 | 2011-05-19 | Beckman Coulter Inc | 全血試料の前処理方法及び核酸増幅方法 |
| CN102245761A (zh) | 2008-11-03 | 2011-11-16 | 卡帕生物系统 | 修饰a型dna聚合酶 |
| FI20095729A0 (fi) * | 2009-06-26 | 2009-06-26 | Finnzymes Oy | Menetelmä deoksiribonukleiinihappojen kvantitatiiviseen PCR-monistukseen PCR-inhibiittoreita sisältävästä näytteestä |
| EP2524036B1 (en) * | 2009-07-31 | 2019-06-12 | Agilent Technologies, Inc. | Thermostable type-a dna polymerase mutants with increased polymerization rate and resistance to inhibitors |
| FI20096013A7 (fi) * | 2009-10-02 | 2011-04-03 | Finnzymes Oy | Menetelmä reaktioseoksen valmistelemiseksi ja tähän liittyvät tuotteet |
| ES2564692T3 (es) | 2010-06-18 | 2016-03-28 | F. Hoffmann-La Roche Ag | ADN polimerasas con discriminación incrementada de no correspondencia 3' |
| EP2582806B1 (en) | 2010-06-18 | 2015-12-16 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| ES2536252T3 (es) | 2010-06-18 | 2015-05-21 | F. Hoffmann-La Roche Ag | ADN polimerasas con diferenciación de desapareamientos 3' aumentada |
| WO2011157434A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| CN103025869B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| EP2582803B1 (en) | 2010-06-18 | 2015-02-25 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| WO2011157436A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| CN103003418B (zh) | 2010-06-18 | 2015-08-26 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| WO2012097318A2 (en) | 2011-01-14 | 2012-07-19 | Kap Abiosystems | Modified dna polymerases for improved amplification |
| US8765436B2 (en) | 2011-02-15 | 2014-07-01 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| EP2675897B1 (en) | 2011-02-15 | 2016-05-11 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
| GB201113430D0 (en) | 2011-08-03 | 2011-09-21 | Fermentas Uab | DNA polymerases |
| US11208636B2 (en) | 2011-08-10 | 2021-12-28 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| EP2742151B1 (en) | 2011-08-10 | 2017-10-25 | Life Technologies Corporation | Polymerase compositions |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| EP2788480B1 (en) | 2011-12-08 | 2019-01-16 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
| WO2013083263A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| US10683537B2 (en) | 2012-10-16 | 2020-06-16 | Dna Polymerase Technology, Inc. | Inhibition-resistant polymerases |
| US9758773B2 (en) * | 2014-02-14 | 2017-09-12 | Agilent Technologies, Inc. | Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood |
| SG11201700456UA (en) | 2014-07-21 | 2017-02-27 | Agency Science Tech & Res | Silica coating on nanoparticles |
| US11091745B2 (en) | 2015-05-12 | 2021-08-17 | Dna Polymerase Technology, Inc. | Mutant polymerases and uses thereof |
| US20230304082A1 (en) * | 2020-05-29 | 2023-09-28 | Genotech Corp. | Dna polymerase variant with improved discrimination of genetic variants |
| US12655479B2 (en) | 2021-10-11 | 2026-06-16 | Dna Polymerase Technology, Inc. | Fast and robust bi-functional mutants of Taq DNA polymerase |
| JP7393070B1 (ja) | 2023-08-04 | 2023-12-06 | 株式会社ゴーフォトン | Ｐｃｒ方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992006188A2 (en) * | 1990-10-05 | 1992-04-16 | Barnes Wayne M | Thermostable dna polymerase |
| EP0590327B1 (en) * | 1992-09-11 | 2003-04-09 | F. Hoffmann-La Roche Ag | Detection of nucleic acids in blood |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US6818431B1 (en) * | 1995-10-18 | 2004-11-16 | Shanghai Mendel Dna Center Co. Ltd. | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
| GB0022458D0 (en) | 2000-09-13 | 2000-11-01 | Medical Res Council | Directed evolution method |
| AU2003279696A1 (en) | 2002-05-14 | 2004-02-23 | Fidelity Systems, Inc. | Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes |
| US20040161767A1 (en) * | 2002-06-28 | 2004-08-19 | Baldwin Brett R. | Detection and quantification of aromatic oxygenase genes by real-time PCR |
| US7417133B2 (en) | 2004-02-27 | 2008-08-26 | Institut Pasteur | Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same |
| US7462475B2 (en) * | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| US20050260606A1 (en) * | 2004-05-20 | 2005-11-24 | Kermekchiev Milko B | Use of whole blood in PCR reactions |
| US20070048748A1 (en) | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| US20070111234A1 (en) * | 2005-09-12 | 2007-05-17 | Christian Birkner | Detection of biological DNA |
-
2007
- 2007-09-14 JP JP2009528513A patent/JP5457184B2/ja not_active Expired - Fee Related
- 2007-09-14 CA CA002664043A patent/CA2664043A1/en not_active Abandoned
- 2007-09-14 AU AU2007296180A patent/AU2007296180A1/en not_active Abandoned
- 2007-09-14 EP EP07842556A patent/EP2061894A4/en not_active Withdrawn
- 2007-09-14 WO PCT/US2007/078571 patent/WO2008034110A2/en not_active Ceased
-
2009
- 2009-03-16 US US12/441,521 patent/US20110027832A1/en not_active Abandoned
-
2013
- 2013-10-04 JP JP2013209506A patent/JP2014054254A/ja active Pending
-
2015
- 2015-06-23 US US14/746,982 patent/US9796965B2/en active Active
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