AU2007296180A1 - Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors - Google Patents

Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors Download PDF

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Publication number
AU2007296180A1
AU2007296180A1 AU2007296180A AU2007296180A AU2007296180A1 AU 2007296180 A1 AU2007296180 A1 AU 2007296180A1 AU 2007296180 A AU2007296180 A AU 2007296180A AU 2007296180 A AU2007296180 A AU 2007296180A AU 2007296180 A1 AU2007296180 A1 AU 2007296180A1
Authority
AU
Australia
Prior art keywords
soil
polymerase
pcr
dye
assay mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2007296180A
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English (en)
Inventor
Milko Kermekchiev
Lyubka Kirilova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNA Polymerase Technology Inc
Original Assignee
DNA Polymerase Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DNA Polymerase Technology Inc filed Critical DNA Polymerase Technology Inc
Publication of AU2007296180A1 publication Critical patent/AU2007296180A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2007296180A 2006-09-14 2007-09-14 Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors Abandoned AU2007296180A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82569206P 2006-09-14 2006-09-14
US60/825,692 2006-09-14
PCT/US2007/078571 WO2008034110A2 (en) 2006-09-14 2007-09-14 Use of taq polymerase mutant enzymes for dna amplification in the presence of pcr inhibitors

Publications (1)

Publication Number Publication Date
AU2007296180A1 true AU2007296180A1 (en) 2008-03-20

Family

ID=39184635

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2007296180A Abandoned AU2007296180A1 (en) 2006-09-14 2007-09-14 Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors

Country Status (6)

Country Link
US (2) US20110027832A1 (enExample)
EP (1) EP2061894A4 (enExample)
JP (2) JP5457184B2 (enExample)
AU (1) AU2007296180A1 (enExample)
CA (1) CA2664043A1 (enExample)
WO (1) WO2008034110A2 (enExample)

Families Citing this family (32)

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US7462475B2 (en) 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
EP2076592B1 (en) 2006-10-23 2017-04-12 Medical Research Council Polymerase
JP2011097828A (ja) * 2008-07-09 2011-05-19 Beckman Coulter Inc 全血試料の前処理方法及び核酸増幅方法
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
FI20095729A0 (fi) * 2009-06-26 2009-06-26 Finnzymes Oy Menetelmä deoksiribonukleiinihappojen kvantitatiiviseen PCR-monistukseen PCR-inhibiittoreita sisältävästä näytteestä
EP2524036B1 (en) * 2009-07-31 2019-06-12 Agilent Technologies, Inc. Thermostable type-a dna polymerase mutants with increased polymerization rate and resistance to inhibitors
FI20096013A7 (fi) * 2009-10-02 2011-04-03 Finnzymes Oy Menetelmä reaktioseoksen valmistelemiseksi ja tähän liittyvät tuotteet
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
US8765436B2 (en) 2011-02-15 2014-07-01 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
EP2675897B1 (en) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
EP2742151B1 (en) 2011-08-10 2017-10-25 Life Technologies Corporation Polymerase compositions
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
US10683537B2 (en) 2012-10-16 2020-06-16 Dna Polymerase Technology, Inc. Inhibition-resistant polymerases
US9758773B2 (en) * 2014-02-14 2017-09-12 Agilent Technologies, Inc. Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood
SG11201700456UA (en) 2014-07-21 2017-02-27 Agency Science Tech & Res Silica coating on nanoparticles
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US20230304082A1 (en) * 2020-05-29 2023-09-28 Genotech Corp. Dna polymerase variant with improved discrimination of genetic variants
US12655479B2 (en) 2021-10-11 2026-06-16 Dna Polymerase Technology, Inc. Fast and robust bi-functional mutants of Taq DNA polymerase
JP7393070B1 (ja) 2023-08-04 2023-12-06 株式会社ゴーフォトン Ｐｃｒ方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992006188A2 (en) * 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
EP0590327B1 (en) * 1992-09-11 2003-04-09 F. Hoffmann-La Roche Ag Detection of nucleic acids in blood
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6818431B1 (en) * 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
AU2003279696A1 (en) 2002-05-14 2004-02-23 Fidelity Systems, Inc. Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
US20040161767A1 (en) * 2002-06-28 2004-08-19 Baldwin Brett R. Detection and quantification of aromatic oxygenase genes by real-time PCR
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
US20050260606A1 (en) * 2004-05-20 2005-11-24 Kermekchiev Milko B Use of whole blood in PCR reactions
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
US20070111234A1 (en) * 2005-09-12 2007-05-17 Christian Birkner Detection of biological DNA

Also Published As

Publication number Publication date
JP2010503413A (ja) 2010-02-04
EP2061894A4 (en) 2010-05-05
CA2664043A1 (en) 2008-03-20
US20110027832A1 (en) 2011-02-03
WO2008034110A2 (en) 2008-03-20
US20150368624A1 (en) 2015-12-24
US9796965B2 (en) 2017-10-24
JP2014054254A (ja) 2014-03-27
WO2008034110A3 (en) 2008-12-24
WO2008034110A9 (en) 2013-09-12
JP5457184B2 (ja) 2014-04-02
EP2061894A2 (en) 2009-05-27

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Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted