JP2008513008A - 細胞材料から核酸を単離するための簡易な方法 - Google Patents

細胞材料から核酸を単離するための簡易な方法 Download PDF

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JP2008513008A
JP2008513008A JP2007532318A JP2007532318A JP2008513008A JP 2008513008 A JP2008513008 A JP 2008513008A JP 2007532318 A JP2007532318 A JP 2007532318A JP 2007532318 A JP2007532318 A JP 2007532318A JP 2008513008 A JP2008513008 A JP 2008513008A
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nucleic acid
solid phase
group
isolation method
sample
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JP2008513008A5 (enExample
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アクハヴァン−タフティ ハシェム
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ネクスゲン ディアグノスティックス エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2007532318A 2004-09-16 2005-07-01 細胞材料から核酸を単離するための簡易な方法 Withdrawn JP2008513008A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/942,491 US20050106602A1 (en) 2003-11-17 2004-09-16 Simplified methods for isolating nucleic acids from cellular materials
PCT/US2005/023608 WO2006036246A2 (en) 2004-09-16 2005-07-01 Simplified methods for isolating nucleic acids from cellular materials

Publications (2)

Publication Number Publication Date
JP2008513008A true JP2008513008A (ja) 2008-05-01
JP2008513008A5 JP2008513008A5 (enExample) 2008-08-14

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JP2007532318A Withdrawn JP2008513008A (ja) 2004-09-16 2005-07-01 細胞材料から核酸を単離するための簡易な方法

Country Status (9)

Country Link
US (1) US20050106602A1 (enExample)
EP (1) EP1799847A4 (enExample)
JP (1) JP2008513008A (enExample)
KR (1) KR20070062557A (enExample)
CN (2) CN101107365A (enExample)
AU (1) AU2005290300A1 (enExample)
CA (1) CA2580700A1 (enExample)
IL (1) IL181974A0 (enExample)
WO (1) WO2006036246A2 (enExample)

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FR2933989B1 (fr) * 2008-07-16 2013-03-08 Commissariat Energie Atomique Procede de purification de microorganismes presents dans des echantillons liquides
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EP2761001B1 (en) 2011-09-26 2018-08-01 Qiagen GmbH Rapid method for isolating extracellular nucleic acids
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DK3030644T3 (da) * 2013-08-05 2019-12-09 Univ Johns Hopkins Fremgangsmåde til fastfaseekstraktion af nukleinsyrer ved anvendelse af en hierarkisk silicananomembran
EP3094390B1 (en) 2014-01-16 2021-07-07 W.R. Grace & CO. - CONN. Affinity chromatography media and chromatography devices
PL3137209T3 (pl) 2014-05-02 2023-01-02 W.R. Grace & Co. - Conn. Funkcjonalizowany materiał nośnikowy i sposoby wytwarzania oraz stosowania funkcjonalizowanego materiału nośnikowego
FR3031063B1 (fr) * 2014-12-30 2017-02-10 Biomerieux Sa Complexe multicouches, procede de fabrication et utilisation du complexe
EP3250690B1 (en) * 2015-01-27 2024-08-07 Circulomics Inc. Method of making a magnetic silica nanomembrane
PL3302784T3 (pl) 2015-06-05 2022-01-17 W.R. Grace & Co.-Conn. Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
EP4031581A4 (en) * 2019-09-18 2023-11-01 Apostle, Inc. APPARATUS SYSTEMS AND METHODS USING CORE-SHELL-SHELL MAGNETIC BEADS
WO2021231607A1 (en) * 2020-05-12 2021-11-18 Godx, Inc. Point of need diagnostic device and methods of use thereof
CN111804280A (zh) * 2020-06-11 2020-10-23 中国科学院合肥物质科学研究院 一种制备磁性纳米氨基复合物的方法、以及核酸提取方法

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Also Published As

Publication number Publication date
EP1799847A4 (en) 2007-12-12
WO2006036246A3 (en) 2007-02-08
CN101107365A (zh) 2008-01-16
EP1799847A2 (en) 2007-06-27
WO2006036246A2 (en) 2006-04-06
CA2580700A1 (en) 2006-04-06
IL181974A0 (en) 2007-07-04
US20050106602A1 (en) 2005-05-19
KR20070062557A (ko) 2007-06-15
AU2005290300A1 (en) 2006-04-06
CN101076603A (zh) 2007-11-21

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