IL181974A0 - Simplified methods for isolating nucleic acids from cellular materials - Google Patents
Simplified methods for isolating nucleic acids from cellular materialsInfo
- Publication number
- IL181974A0 IL181974A0 IL181974A IL18197407A IL181974A0 IL 181974 A0 IL181974 A0 IL 181974A0 IL 181974 A IL181974 A IL 181974A IL 18197407 A IL18197407 A IL 18197407A IL 181974 A0 IL181974 A0 IL 181974A0
- Authority
- IL
- Israel
- Prior art keywords
- nucleic acids
- isolating nucleic
- cellular materials
- simplified methods
- simplified
- Prior art date
Links
- 230000001413 cellular effect Effects 0.000 title 1
- 239000000463 material Substances 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/942,491 US20050106602A1 (en) | 2003-11-17 | 2004-09-16 | Simplified methods for isolating nucleic acids from cellular materials |
| PCT/US2005/023608 WO2006036246A2 (en) | 2004-09-16 | 2005-07-01 | Simplified methods for isolating nucleic acids from cellular materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL181974A0 true IL181974A0 (en) | 2007-07-04 |
Family
ID=36119320
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL181974A IL181974A0 (en) | 2004-09-16 | 2007-03-15 | Simplified methods for isolating nucleic acids from cellular materials |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20050106602A1 (enExample) |
| EP (1) | EP1799847A4 (enExample) |
| JP (1) | JP2008513008A (enExample) |
| KR (1) | KR20070062557A (enExample) |
| CN (2) | CN101107365A (enExample) |
| AU (1) | AU2005290300A1 (enExample) |
| CA (1) | CA2580700A1 (enExample) |
| IL (1) | IL181974A0 (enExample) |
| WO (1) | WO2006036246A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060234251A1 (en) | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
| US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
| US20090048439A1 (en) * | 2007-08-06 | 2009-02-19 | Weisburg William G | Isolation of nucleic acids molecules using modified solid supports |
| FR2933989B1 (fr) * | 2008-07-16 | 2013-03-08 | Commissariat Energie Atomique | Procede de purification de microorganismes presents dans des echantillons liquides |
| GB201010237D0 (en) | 2010-06-18 | 2010-07-21 | Lgc Ltd | Methods and apparatuses |
| EP4159857A1 (en) * | 2011-09-26 | 2023-04-05 | QIAGEN GmbH | Rapid method for isolating extracellular nucleic acids |
| WO2014015098A1 (en) | 2012-07-18 | 2014-01-23 | Siemens Healthcare Diagnostics Inc. | A method of normalizing biological samples |
| JP6360482B2 (ja) | 2012-09-17 | 2018-07-18 | ダブリュー・アール・グレース・アンド・カンパニー−コーンW R Grace & Co−Conn | クロマトグラフィー担体及びデバイス |
| WO2014043644A1 (en) | 2012-09-17 | 2014-03-20 | Alltech Associates, Inc. | Functionalized particulate support material and methods of making and using the same |
| AU2014305085A1 (en) * | 2013-08-05 | 2016-02-25 | The Johns Hopkins University | Fabrication of hierarchical silica nanomembranes and uses thereof for solid phase extraction of nucleic acids |
| ES2887110T3 (es) | 2014-01-16 | 2021-12-21 | Grace W R & Co | Medios para cromatografía de afinidad y dispositivos para cromatografía |
| US11389783B2 (en) | 2014-05-02 | 2022-07-19 | W.R. Grace & Co.-Conn. | Functionalized support material and methods of making and using functionalized support material |
| FR3031063B1 (fr) * | 2014-12-30 | 2017-02-10 | Biomerieux Sa | Complexe multicouches, procede de fabrication et utilisation du complexe |
| WO2016123101A1 (en) | 2015-01-27 | 2016-08-04 | Circulomics Inc. | Hierarchical silica lamella for magnetic nucleic acid extraction |
| CN107921407B (zh) | 2015-06-05 | 2022-07-05 | 格雷斯公司 | 生物处理吸附澄清剂及其制备和使用方法 |
| EP3307886B1 (en) | 2015-06-10 | 2021-03-24 | Qiagen GmbH | Method for isolating extracellular nucleic acids using anion exchange particles |
| CN109563539A (zh) | 2016-06-15 | 2019-04-02 | 慕尼黑路德维希马克西米利安斯大学 | 使用dna纳米技术的单分子检测或定量 |
| WO2021055083A1 (en) * | 2019-09-18 | 2021-03-25 | Apostle, Inc. | Apparatuses systems and methods using core-shell-shell magnetic beads |
| US20230227888A1 (en) * | 2020-05-12 | 2023-07-20 | Godx, Inc. | Point of need diagnostic device and methods of use thereof |
| CN111804280A (zh) * | 2020-06-11 | 2020-10-23 | 中国科学院合肥物质科学研究院 | 一种制备磁性纳米氨基复合物的方法、以及核酸提取方法 |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4297337A (en) * | 1979-04-13 | 1981-10-27 | Corning Glass Works | Solid-phase immunoassays using magnetic glass |
| US4233169A (en) * | 1979-04-13 | 1980-11-11 | Corning Glass Works | Porous magnetic glass structure |
| US4395271A (en) * | 1979-04-13 | 1983-07-26 | Corning Glass Works | Method for making porous magnetic glass and crystal-containing structures |
| DE3211309A1 (de) * | 1982-03-26 | 1983-09-29 | Metin Dipl.-Ing. 6100 Darmstadt Colpan | Chromatographisches verfahren zur isolierung von makromolekuelen |
| NO155316C (no) * | 1982-04-23 | 1987-03-11 | Sintef | Fremgangsmaate for fremstilling av magnetiske polymerpartikler. |
| US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| AU604684B2 (en) * | 1986-03-20 | 1991-01-03 | Gen-Probe Incorporated | Method for releasing RNA and DNA from cells |
| DE3639949A1 (de) * | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | Verfahren zur trennung von langkettigen nukleinsaeuren |
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| US5599667A (en) * | 1987-03-02 | 1997-02-04 | Gen-Probe Incorporated | Polycationic supports and nucleic acid purification separation and hybridization |
| US5395688A (en) | 1987-10-26 | 1995-03-07 | Baxter Diagnostics Inc. | Magnetically responsive fluorescent polymer particles |
| US5091206A (en) * | 1987-10-26 | 1992-02-25 | Baxter Diagnostics Inc. | Process for producing magnetically responsive polymer particles and application thereof |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US4997932A (en) * | 1989-11-13 | 1991-03-05 | Boehringer Mannheim Corporation | Method and kit for purifying nucleic acids |
| US5665582A (en) * | 1990-10-29 | 1997-09-09 | Dekalb Genetics Corp. | Isolation of biological materials |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5411730A (en) * | 1993-07-20 | 1995-05-02 | Research Corporation Technologies, Inc. | Magnetic microparticles |
| US5986076A (en) * | 1994-05-11 | 1999-11-16 | Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
| US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5942425A (en) | 1996-03-12 | 1999-08-24 | Walters; Adriann H. | Method to access nucleic acids from cells |
| US5900481A (en) * | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
| US6060246A (en) * | 1996-11-15 | 2000-05-09 | Avi Biopharma, Inc. | Reagent and method for isolation and detection of selected nucleic acid sequences |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| ATE347615T1 (de) * | 1997-04-16 | 2006-12-15 | Applera Corp | Nukleinsäuresammlung |
| DE69827745T2 (de) * | 1997-06-12 | 2005-11-10 | Sloan-Kettering Institute For Cancer Research | Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase |
| GB9717173D0 (en) * | 1997-08-13 | 1997-10-22 | Akzo Nobel Nv | Solid phase supports |
| DE19736366A1 (de) * | 1997-08-21 | 1999-02-25 | Ernst Prof Dr Bayer | Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln |
| US6120985A (en) * | 1997-10-31 | 2000-09-19 | Bbi Bioseq, Inc. | Pressure-enhanced extraction and purification |
| US6214618B1 (en) * | 1998-04-07 | 2001-04-10 | Solohill Engineering, Inc. | Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior |
| US6534262B1 (en) * | 1998-05-14 | 2003-03-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6780327B1 (en) * | 1999-02-25 | 2004-08-24 | Pall Corporation | Positively charged membrane |
| US6514700B1 (en) * | 1999-04-30 | 2003-02-04 | Aclara Biosciences, Inc. | Nucleic acid detection using degradation of a tagged sequence |
| ES2228520T3 (es) * | 1999-05-04 | 2005-04-16 | Ortho-Clinical Diagnostics, Inc. | Capatura rapida y eficaz de adn de una muestra sin usar reactivo de lisis celular. |
| GB2367818A (en) * | 2000-10-11 | 2002-04-17 | Kalibrant Ltd | A linker and a method of chemical synthesis |
| US6803200B2 (en) * | 2000-12-12 | 2004-10-12 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
| JP4399164B2 (ja) | 2001-02-16 | 2010-01-13 | プロメガ・コーポレーション | 核酸の磁気単離および精製の方法 |
| EP1243649A1 (de) | 2001-03-23 | 2002-09-25 | GeneScan Europe AG | Verfahren zur Isolierung von DNA |
| US6746608B2 (en) * | 2001-06-12 | 2004-06-08 | Prometic Biosciences, Inc. | Use of adsorbent polymer particles in DNA separation |
| EP1563091B2 (en) * | 2002-10-04 | 2012-06-27 | GE Healthcare Bio-Sciences Corp. | Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems |
| US20040121336A1 (en) * | 2002-12-20 | 2004-06-24 | Greenfield I Lawrence | Method for generating multiple samples containing a predetermined amount of nucleic acid |
| ATE466105T1 (de) * | 2003-09-12 | 2010-05-15 | Biocontrol Systems Inc | Verfahren, zusammensetzungen und kits zur anreicherung und zum nachweis von mikroorganismen |
| US20050106589A1 (en) * | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Compositions and methods for releasing nucleic acids from solid phase binding materials |
| US20060234251A1 (en) * | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
-
2004
- 2004-09-16 US US10/942,491 patent/US20050106602A1/en not_active Abandoned
-
2005
- 2005-07-01 CA CA002580700A patent/CA2580700A1/en not_active Abandoned
- 2005-07-01 AU AU2005290300A patent/AU2005290300A1/en not_active Abandoned
- 2005-07-01 KR KR1020077008632A patent/KR20070062557A/ko not_active Withdrawn
- 2005-07-01 JP JP2007532318A patent/JP2008513008A/ja not_active Withdrawn
- 2005-07-01 WO PCT/US2005/023608 patent/WO2006036246A2/en not_active Ceased
- 2005-07-01 CN CNA2005800366286A patent/CN101107365A/zh active Pending
- 2005-07-01 EP EP05768762A patent/EP1799847A4/en not_active Withdrawn
- 2005-07-05 CN CNA2005800366290A patent/CN101076603A/zh active Pending
-
2007
- 2007-03-15 IL IL181974A patent/IL181974A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2580700A1 (en) | 2006-04-06 |
| JP2008513008A (ja) | 2008-05-01 |
| AU2005290300A1 (en) | 2006-04-06 |
| US20050106602A1 (en) | 2005-05-19 |
| EP1799847A2 (en) | 2007-06-27 |
| WO2006036246A3 (en) | 2007-02-08 |
| KR20070062557A (ko) | 2007-06-15 |
| CN101076603A (zh) | 2007-11-21 |
| WO2006036246A2 (en) | 2006-04-06 |
| EP1799847A4 (en) | 2007-12-12 |
| CN101107365A (zh) | 2008-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL181974A0 (en) | Simplified methods for isolating nucleic acids from cellular materials | |
| EP1954667A4 (en) | METHODS FOR SEPARATING PROPARGYL AMINOINDANES | |
| EP1856291A4 (en) | NUCLEIC ACIDS AND PROTEINS, AND METHODS OF MAKING AND USING SAME | |
| EP1725967A4 (en) | METHOD FOR GENETIC ANALYSIS | |
| IL177404A0 (en) | Methods for preparing t-cells | |
| PL1960520T3 (pl) | Sposób izolowania kwasów nukleinowych z dowolnego materiału wyjściowego | |
| IL180504A0 (en) | Methods for isolating nucleic acids from cleavable solid phases | |
| EP1806393A4 (en) | MICROREACTOR FOR GENETIC TEST | |
| GB0522303D0 (en) | Culture method | |
| ZA200703239B (en) | Surface support method | |
| ZA200703240B (en) | Surface support method | |
| GB0427023D0 (en) | Assay method | |
| IL179901A0 (en) | Process for protein isolation | |
| EP1664282A4 (en) | METHOD FOR ISOLATING HEPATOCYTES | |
| EP1862801A4 (en) | TEST METHOD CARRIED OUT WITH RAPPORTEUR GENE | |
| GB2419594B (en) | Method for stabilizing or isolating nucleic acids | |
| IL181975A0 (en) | Methods for isolating nucleic acids from biological and cellular materials | |
| GB2415782B (en) | Method for obtaining aptamer using microarrays | |
| TWI315261B (en) | Method for transferring-printing | |
| ZA200700167B (en) | Process for protein isolation | |
| GB0502065D0 (en) | Assay method | |
| GB0500703D0 (en) | Molecular biology method | |
| EP1968953A4 (en) | METHOD OF ISOLATING (R) -TOFISOPAM | |
| GB0406800D0 (en) | Microarray method | |
| MX268973B (es) | Metodo para mejorar la durabilidad ambiental de los materiales. |