AU2005290300A1 - Simplified methods for isolating nucleic acids from cellular materials - Google Patents

Simplified methods for isolating nucleic acids from cellular materials Download PDF

Info

Publication number
AU2005290300A1
AU2005290300A1 AU2005290300A AU2005290300A AU2005290300A1 AU 2005290300 A1 AU2005290300 A1 AU 2005290300A1 AU 2005290300 A AU2005290300 A AU 2005290300A AU 2005290300 A AU2005290300 A AU 2005290300A AU 2005290300 A1 AU2005290300 A1 AU 2005290300A1
Authority
AU
Australia
Prior art keywords
solid phase
nucleic acid
nucleic acids
groups
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2005290300A
Other languages
English (en)
Inventor
Hashem Akhavan-Tafti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nexgen Diagnostics LLC
Original Assignee
Nexgen Diagnostics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexgen Diagnostics LLC filed Critical Nexgen Diagnostics LLC
Publication of AU2005290300A1 publication Critical patent/AU2005290300A1/en
Assigned to NEXGEN DIAGNOSTICS, LLC. reassignment NEXGEN DIAGNOSTICS, LLC. Request for Assignment Assignors: LUMIGEN, INC.
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2005290300A 2004-09-16 2005-07-01 Simplified methods for isolating nucleic acids from cellular materials Abandoned AU2005290300A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/942,491 US20050106602A1 (en) 2003-11-17 2004-09-16 Simplified methods for isolating nucleic acids from cellular materials
US10/942,491 2004-09-16
PCT/US2005/023608 WO2006036246A2 (en) 2004-09-16 2005-07-01 Simplified methods for isolating nucleic acids from cellular materials

Publications (1)

Publication Number Publication Date
AU2005290300A1 true AU2005290300A1 (en) 2006-04-06

Family

ID=36119320

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005290300A Abandoned AU2005290300A1 (en) 2004-09-16 2005-07-01 Simplified methods for isolating nucleic acids from cellular materials

Country Status (9)

Country Link
US (1) US20050106602A1 (enExample)
EP (1) EP1799847A4 (enExample)
JP (1) JP2008513008A (enExample)
KR (1) KR20070062557A (enExample)
CN (2) CN101107365A (enExample)
AU (1) AU2005290300A1 (enExample)
CA (1) CA2580700A1 (enExample)
IL (1) IL181974A0 (enExample)
WO (1) WO2006036246A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
FR2933989B1 (fr) * 2008-07-16 2013-03-08 Commissariat Energie Atomique Procede de purification de microorganismes presents dans des echantillons liquides
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
EP4159857A1 (en) 2011-09-26 2023-04-05 QIAGEN GmbH Rapid method for isolating extracellular nucleic acids
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples
PL2811983T3 (pl) 2012-09-17 2019-10-31 Grace W R & Co Funkcjonalizowany, złożony z cząstek materiał nośnikowy i sposoby jego wytwarzania i zastosowania
ES2868093T3 (es) 2012-09-17 2021-10-21 Grace W R & Co Medio y dispositivos de cromatografía
DK3030644T3 (da) * 2013-08-05 2019-12-09 Univ Johns Hopkins Fremgangsmåde til fastfaseekstraktion af nukleinsyrer ved anvendelse af en hierarkisk silicananomembran
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
ES2929099T3 (es) 2014-05-02 2022-11-24 Grace W R & Co Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado
FR3031063B1 (fr) * 2014-12-30 2017-02-10 Biomerieux Sa Complexe multicouches, procede de fabrication et utilisation du complexe
US10737276B2 (en) 2015-01-27 2020-08-11 Circulomics Inc. Hierarchical silica lamella for magnetic nucleic acid extraction
BR112017026193B1 (pt) 2015-06-05 2021-09-14 W.R. Grace & Co-Conn Adsorventes, método de produção dos adsorventes e uso dos adsorventes
CN107690481B (zh) 2015-06-10 2021-08-13 凯杰有限公司 利用阴离子交换粒子分离细胞外核酸的方法
US11513076B2 (en) 2016-06-15 2022-11-29 Ludwig-Maximilians-Universität München Single molecule detection or quantification using DNA nanotechnology
EP4031581A4 (en) * 2019-09-18 2023-11-01 Apostle, Inc. APPARATUS SYSTEMS AND METHODS USING CORE-SHELL-SHELL MAGNETIC BEADS
US20230227888A1 (en) * 2020-05-12 2023-07-20 Godx, Inc. Point of need diagnostic device and methods of use thereof
CN111804280A (zh) * 2020-06-11 2020-10-23 中国科学院合肥物质科学研究院 一种制备磁性纳米氨基复合物的方法、以及核酸提取方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4297337A (en) * 1979-04-13 1981-10-27 Corning Glass Works Solid-phase immunoassays using magnetic glass
US4233169A (en) * 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US4395271A (en) * 1979-04-13 1983-07-26 Corning Glass Works Method for making porous magnetic glass and crystal-containing structures
DE3211309A1 (de) * 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
NO155316C (no) * 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
CA1296604C (en) * 1986-03-20 1992-03-03 Kathleen Murphy Method for releasing rna and dna from cells
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US5091206A (en) * 1987-10-26 1992-02-25 Baxter Diagnostics Inc. Process for producing magnetically responsive polymer particles and application thereof
US5395688A (en) 1987-10-26 1995-03-07 Baxter Diagnostics Inc. Magnetically responsive fluorescent polymer particles
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5665582A (en) * 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5411730A (en) * 1993-07-20 1995-05-02 Research Corporation Technologies, Inc. Magnetic microparticles
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5942425A (en) 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
EP1003908B1 (en) * 1997-04-16 2006-12-06 Applera Corporation Nucleic acid archiving
ATE283353T1 (de) * 1997-06-12 2004-12-15 Sloan Kettering Inst Cancer Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase
GB9717173D0 (en) * 1997-08-13 1997-10-22 Akzo Nobel Nv Solid phase supports
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6214618B1 (en) * 1998-04-07 2001-04-10 Solohill Engineering, Inc. Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6780327B1 (en) * 1999-02-25 2004-08-24 Pall Corporation Positively charged membrane
US6514700B1 (en) * 1999-04-30 2003-02-04 Aclara Biosciences, Inc. Nucleic acid detection using degradation of a tagged sequence
DE60014399T2 (de) * 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
GB2367818A (en) * 2000-10-11 2002-04-17 Kalibrant Ltd A linker and a method of chemical synthesis
WO2002048385A2 (en) * 2000-12-12 2002-06-20 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
EP2090655B1 (en) 2001-02-16 2013-05-08 Promega Corporation Magnetic isolation and purification of nucleic acids
EP1243649A1 (de) 2001-03-23 2002-09-25 GeneScan Europe AG Verfahren zur Isolierung von DNA
US6746608B2 (en) * 2001-06-12 2004-06-08 Prometic Biosciences, Inc. Use of adsorbent polymer particles in DNA separation
AU2003279134A1 (en) * 2002-10-04 2004-05-04 Whatman, Inc. Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems
US20040121336A1 (en) * 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
NZ545469A (en) * 2003-09-12 2009-11-27 Biocontrol Systems Inc Methods, compositions, and kits for the concentration and detection of microorganisms without a separate DNA extraction step
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples

Also Published As

Publication number Publication date
EP1799847A4 (en) 2007-12-12
EP1799847A2 (en) 2007-06-27
IL181974A0 (en) 2007-07-04
WO2006036246A3 (en) 2007-02-08
CN101076603A (zh) 2007-11-21
CN101107365A (zh) 2008-01-16
WO2006036246A2 (en) 2006-04-06
US20050106602A1 (en) 2005-05-19
JP2008513008A (ja) 2008-05-01
KR20070062557A (ko) 2007-06-15
CA2580700A1 (en) 2006-04-06

Similar Documents

Publication Publication Date Title
US20050106602A1 (en) Simplified methods for isolating nucleic acids from cellular materials
US20050136477A1 (en) Methods for isolating nucleic acids from biological and cellular materials
EP1872131A2 (en) Methods of enhancing isolation of rna from biological samples
US20050106589A1 (en) Compositions and methods for releasing nucleic acids from solid phase binding materials
CA2642883A1 (en) Methods of extracting nucleic acids
US20050106576A1 (en) Methods of using cleavable solid phases for isolating nucleic acids
US20050106577A1 (en) Cleavable solid phases for isolating nucleic acids

Legal Events

Date Code Title Description
PC1 Assignment before grant (sect. 113)

Owner name: NEXGEN DIAGNOSTICS, LLC.

Free format text: FORMER APPLICANT(S): LUMIGEN, INC.

MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application