ATE283353T1 - Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase - Google Patents

Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase

Info

Publication number
ATE283353T1
ATE283353T1 AT98932755T AT98932755T ATE283353T1 AT E283353 T1 ATE283353 T1 AT E283353T1 AT 98932755 T AT98932755 T AT 98932755T AT 98932755 T AT98932755 T AT 98932755T AT E283353 T1 ATE283353 T1 AT E283353T1
Authority
AT
Austria
Prior art keywords
dna
catalysed
covalent binding
rna strands
vaccinia topoisomerase
Prior art date
Application number
AT98932755T
Other languages
English (en)
Inventor
Stewart Shuman
Joann Sekiguchi
Joseph Fernandez
Robert Marcil
James Hoeffler
John Comisky
Original Assignee
Sloan Kettering Inst Cancer
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sloan Kettering Inst Cancer, Invitrogen Corp filed Critical Sloan Kettering Inst Cancer
Application granted granted Critical
Publication of ATE283353T1 publication Critical patent/ATE283353T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)
    • C12Y599/01002—DNA topoisomerase (5.99.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT98932755T 1997-06-12 1998-06-12 Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase ATE283353T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4940597P 1997-06-12 1997-06-12
PCT/US1998/012372 WO1998056943A1 (en) 1997-06-12 1998-06-12 Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase

Publications (1)

Publication Number Publication Date
ATE283353T1 true ATE283353T1 (de) 2004-12-15

Family

ID=21959648

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98932755T ATE283353T1 (de) 1997-06-12 1998-06-12 Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase

Country Status (8)

Country Link
US (4) US6653106B1 (de)
EP (1) EP0920526B1 (de)
JP (1) JP4447664B2 (de)
AT (1) ATE283353T1 (de)
AU (1) AU746657B2 (de)
CA (1) CA2263716C (de)
DE (1) DE69827745T2 (de)
WO (1) WO1998056943A1 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
DE69827745T2 (de) 1997-06-12 2005-11-10 Sloan-Kettering Institute For Cancer Research Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase
AU774306B2 (en) * 1999-01-05 2004-06-24 Trustees Of Boston University Improved nucleic acid cloning
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
AU3397800A (en) * 1999-03-19 2000-10-09 Invitrogen Corporation Methods of obtaining full-length nucleic acid sequences using (e. coli) topoisomerase iii and its homologs
DE19916227A1 (de) * 1999-04-10 2000-11-02 Merlin Mikrobiolog Diag Gmbh Verfahren zur genotypischen Klassifizierung
US7517688B2 (en) 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
US7078501B2 (en) * 2000-02-25 2006-07-18 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
WO2001062892A2 (en) 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU8835601A (en) * 2000-08-21 2002-03-04 Invitrogen Corp Methods and reagents for molecular cloning
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
EP1339875B1 (de) 2000-12-08 2012-06-20 Life Technologies Corporation Zusammensetzungen und verfahren zur schnellen erzeugung rekombinanter nukleinsäuremoleküle
US20060008817A1 (en) * 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
EP1687323A4 (de) 2003-08-08 2009-08-12 Life Technologies Corp Verfahren und zusammensetzungen zum nahtlosen klonieren von nukleinsäuremolekülen
US20050106602A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US7707707B2 (en) * 2005-01-04 2010-05-04 Hitachi Global Storage Technologies Netherlands B.V. Method for providing a temporary deep shunt on wafer structures for electrostatic discharge protection during processing
US7881933B2 (en) * 2007-03-23 2011-02-01 Verizon Patent And Licensing Inc. Age determination using speech
US8323930B2 (en) * 2007-07-28 2012-12-04 Dna Twopointo, Inc. Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase
CN101424640B (zh) * 2007-11-02 2012-07-25 江苏命码生物科技有限公司 血清中微小核糖核酸的检测方法和用于检测的试剂盒、生物芯片及其制作和应用方法
US20120184020A1 (en) 2009-07-09 2012-07-19 Verdezyne, Inc. Engineered microorganisms with enhanced fermentation activity
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
US20110294996A1 (en) * 2010-05-31 2011-12-01 Scheiber Lane Bernard Quantum unit of inheritance
CA2841794C (en) 2011-07-06 2019-08-13 Verdezyne, Inc. Genetically modified yeast with increased alcohol dehydrogenase activity for preparing a fatty dicarboxylic acid
US9465519B2 (en) 2011-12-21 2016-10-11 Life Technologies Corporation Methods and systems for in silico experimental designing and performing a biological workflow
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples
CA2895130A1 (en) 2012-12-19 2014-06-26 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
CN105189731A (zh) 2012-12-19 2015-12-23 沃德金有限公司 制备脂肪二羧酸的生物学方法
US9677984B2 (en) * 2014-01-31 2017-06-13 University Of Maryland Pulsed-field differential mobility analyzer system and method for separating particles and measuring shape parameters for non-spherical particles
US11274333B2 (en) 2015-05-29 2022-03-15 Molecular Cloning Laboratories (MCLAB) LLC Compositions and methods for preparing sequencing libraries
KR20180123062A (ko) * 2016-02-29 2018-11-14 이리디아, 인크. 정보 저장을 위한 방법, 조성물, 및 장치
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
US10438662B2 (en) 2016-02-29 2019-10-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10859562B2 (en) 2016-02-29 2020-12-08 Iridia, Inc. Methods, compositions, and devices for information storage
CN106434719A (zh) * 2016-09-27 2017-02-22 北京擎科新业生物技术有限公司 基于牛痘拓扑异构酶i的零背景载体构建方法
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
US11655465B1 (en) 2019-05-02 2023-05-23 Iridia, Inc. Enzymes and systems for synthesizing DNA
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
CN112562800A (zh) * 2020-12-15 2021-03-26 长安大学 一种可寻址的dna多面体拓扑结构的图染色及重组方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
EP0178314A1 (de) 1984-04-26 1986-04-23 The Australian National University Klonierungsverfahren für doppelstrang-dns und dessen verwendung bei der herstellung viraler genklone
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
EP0260032B1 (de) * 1986-09-08 1994-01-26 Ajinomoto Co., Inc. Verbindungen zur Spaltung von RNS an eine spezifische Position, Oligomere, verwendet bei der Herstellung dieser Verbindungen und Ausgangsprodukte für die Synthese dieser Oligomere
CA2005252C (en) 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
JP2994010B2 (ja) 1989-10-31 1999-12-27 財団法人相模中央化学研究所 クローニングベクタープラスミド、それから誘導されるベクタープライマー及びそれを用いたｃＤＮＡバンクの作製方法
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法
DE4319468C1 (de) * 1993-06-11 1994-11-17 Mueller Manfred W Verfahren zum Herstellen eines zu einem Ribonukleinsäure (RNA)-Molekül komplementären Desoxyribonukleinsäure(DNA)-Stranges (cDNA-Stranges), sowie die Verwendung des Verfahrens zur Analyse von RNA-Molekülen
US5766891A (en) * 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
FR2733762B1 (fr) * 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5746997A (en) 1996-10-02 1998-05-05 Epoch Pharmaceuticals, Inc. Radiohalogenation of oligonucleotides via trialkylstannylaryl conjugates
JP3441899B2 (ja) 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
US5942422A (en) 1996-11-14 1999-08-24 The Trustees Of Columbia University In The City Of New York Method for generating a directed, recombinant fusion nucleic acid
WO1998055502A1 (en) 1997-06-04 1998-12-10 Smithkline Beecham Corporation METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs
DE69827745T2 (de) 1997-06-12 2005-11-10 Sloan-Kettering Institute For Cancer Research Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase
AU5584999A (en) 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
AU3397800A (en) 1999-03-19 2000-10-09 Invitrogen Corporation Methods of obtaining full-length nucleic acid sequences using (e. coli) topoisomerase iii and its homologs
WO2000056914A1 (en) 1999-03-24 2000-09-28 Gene Therapy Systems, Inc. Method for generating transcriptionally active dna fragments
US7078501B2 (en) 2000-02-25 2006-07-18 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
WO2001062892A2 (en) 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
AU8835601A (en) 2000-08-21 2002-03-04 Invitrogen Corp Methods and reagents for molecular cloning

Also Published As

Publication number Publication date
JP2001507241A (ja) 2001-06-05
AU746657B2 (en) 2002-05-02
US20040058417A1 (en) 2004-03-25
WO1998056943A9 (en) 1999-04-15
DE69827745T2 (de) 2005-11-10
US7662556B2 (en) 2010-02-16
US20120322109A1 (en) 2012-12-20
US8192932B2 (en) 2012-06-05
DE69827745D1 (de) 2004-12-30
WO1998056943A1 (en) 1998-12-17
CA2263716C (en) 2007-09-25
EP0920526A1 (de) 1999-06-09
JP4447664B2 (ja) 2010-04-07
EP0920526A4 (de) 2001-05-30
US20100317064A1 (en) 2010-12-16
US6653106B1 (en) 2003-11-25
EP0920526B1 (de) 2004-11-24
CA2263716A1 (en) 1998-12-17
AU8256598A (en) 1998-12-30

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