WO2002077274A3 - Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice. - Google Patents

Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice. Download PDF

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Publication number
WO2002077274A3
WO2002077274A3 PCT/FR2002/001034 FR0201034W WO02077274A3 WO 2002077274 A3 WO2002077274 A3 WO 2002077274A3 FR 0201034 W FR0201034 W FR 0201034W WO 02077274 A3 WO02077274 A3 WO 02077274A3
Authority
WO
WIPO (PCT)
Prior art keywords
double stranded
stranded dna
triple helix
purifying
target sequences
Prior art date
Application number
PCT/FR2002/001034
Other languages
English (en)
Other versions
WO2002077274A2 (fr
Inventor
Francis Blanche
Beatrice Cameron
Original Assignee
Gencell Sa
Francis Blanche
Beatrice Cameron
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR0103953A external-priority patent/FR2822476B1/fr
Priority to JP2002575316A priority Critical patent/JP4444564B2/ja
Priority to MXPA03008642A priority patent/MXPA03008642A/es
Priority to KR10-2003-7012346A priority patent/KR20030088464A/ko
Priority to AU2002253261A priority patent/AU2002253261B2/en
Priority to EP02722374A priority patent/EP1370692A2/fr
Priority to HU0303632A priority patent/HUP0303632A3/hu
Priority to NZ528507A priority patent/NZ528507A/en
Application filed by Gencell Sa, Francis Blanche, Beatrice Cameron filed Critical Gencell Sa
Priority to BR0208292-6A priority patent/BR0208292A/pt
Priority to IL15801702A priority patent/IL158017A0/xx
Priority to CA002440133A priority patent/CA2440133A1/fr
Publication of WO2002077274A2 publication Critical patent/WO2002077274A2/fr
Priority to IL158017A priority patent/IL158017A/en
Priority to NO20034184A priority patent/NO20034184L/no
Publication of WO2002077274A3 publication Critical patent/WO2002077274A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention a pour objet de nouvelles séquences cibles d'ADN double brin susceptibles d'interagir avec un troisième brin et de former une triple hélice stable. La présente invention concerne en outre un procédé de purification d'une molécule d'ADN double brin selon lequel on met en contact une solution contenant ladite molécule d'ADN avec un troisième brin d'ADN capable de former par hybridation une structure triple hélice avec une séquence cible d'ADN double brin portée par ladite molécule d'ADN.
PCT/FR2002/001034 2001-03-23 2002-03-25 Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice. WO2002077274A2 (fr)

Priority Applications (12)

Application Number Priority Date Filing Date Title
CA002440133A CA2440133A1 (fr) 2001-03-23 2002-03-25 Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice
NZ528507A NZ528507A (en) 2001-03-23 2002-03-25 Processes for purifying and for detecting target double-stranded DNA sequences by triple helix interaction
KR10-2003-7012346A KR20030088464A (ko) 2001-03-23 2002-03-25 2본쇄 ｄｎａ 표적 서열을 3중 나선 상호작용에 의해 정제및 검출하는 방법
AU2002253261A AU2002253261B2 (en) 2001-03-23 2002-03-25 Methods for purifying and detecting double stranded DNA target sequences by triple helix interaction
EP02722374A EP1370692A2 (fr) 2001-03-23 2002-03-25 Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice.
HU0303632A HUP0303632A3 (en) 2001-03-23 2002-03-25 Methods for purifying and detecting double stranded dna target sequences by triple helix interaction
BR0208292-6A BR0208292A (pt) 2001-03-23 2002-03-25 Processos de purificação de uma molécula de dna de duplo filamento e de detecção de um dna de duplo filamento e dna de duplo filamento purificado
JP2002575316A JP4444564B2 (ja) 2001-03-23 2002-03-25 三重螺旋相互作用によるターゲット二本鎖ｄｎａ配列の精製及び検出方法
MXPA03008642A MXPA03008642A (es) 2001-03-23 2002-03-25 Metodos de purificacion y de deteccion de secuencias objetivo de adn de doble hebra mediante interaccion de triple helice.
IL15801702A IL158017A0 (en) 2001-03-23 2002-03-25 Methods for purifying and detecting double stranded dna target sequences by triple helix interaction
IL158017A IL158017A (en) 2001-03-23 2003-09-18 Processes for purifying and for detecting target double-stranded dna sequences by triple helix interaction
NO20034184A NO20034184L (no) 2001-03-23 2003-09-19 Fremgangsmate for rensing og detektering av dobbeltstrengede mal-DNA-sekvenser ved trippel-heliks-interaksjon

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR0103953A FR2822476B1 (fr) 2001-03-23 2001-03-23 Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice
FR01/03953 2001-03-23
US28527201P 2001-04-23 2001-04-23
US60/285,272 2001-04-23

Publications (2)

Publication Number Publication Date
WO2002077274A2 WO2002077274A2 (fr) 2002-10-03
WO2002077274A3 true WO2002077274A3 (fr) 2003-10-02

Family

ID=26212934

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/001034 WO2002077274A2 (fr) 2001-03-23 2002-03-25 Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice.

Country Status (13)

Country Link
EP (1) EP1370692A2 (fr)
JP (1) JP4444564B2 (fr)
KR (2) KR20030088464A (fr)
CN (1) CN1498276A (fr)
BR (1) BR0208292A (fr)
CA (1) CA2440133A1 (fr)
HU (1) HUP0303632A3 (fr)
IL (2) IL158017A0 (fr)
MX (1) MXPA03008642A (fr)
NO (1) NO20034184L (fr)
NZ (1) NZ528507A (fr)
PL (1) PL365208A1 (fr)
WO (1) WO2002077274A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1737945T1 (sl) 2004-04-19 2011-05-31 Aventis Pharma Sa Postopek za čiščenje plazmidne DNA
MX2012005217A (es) * 2009-11-06 2012-07-23 Univ Hong Kong Chinese Analisis genomico a base de tamaño.
EP2407552B1 (fr) * 2010-07-13 2012-11-14 Cnrs Procédé d'isolation des protéines liées à un type de séquence d'acide nucléique d'intérêt
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US11248257B2 (en) 2017-08-25 2022-02-15 Zoetis Services Llc Method of immobilizing a nucleic acid probe to a solid support
CN109959691B (zh) * 2019-04-15 2021-06-08 济南大学 一种基于级联光电活性材料和三螺旋分子开关检测核酸的方法
CN116396963B (zh) * 2023-04-12 2023-10-13 南通大学 一类抑制血管内皮生长因子a表达的三链形成寡核苷酸及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011390A1 (fr) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Detection de sequences d'acide nucleique par la formation d'une triple helice de l'adn
WO1996018744A2 (fr) * 1994-12-16 1996-06-20 Rhone-Poulenc Rorer S.A. Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
WO1996026270A1 (fr) * 1995-02-23 1996-08-29 Rhone-Poulenc Rorer S.A. Molecules d'adn, preparation et utilisation en therapie genique
WO1997035002A1 (fr) * 1996-03-21 1997-09-25 Rhone-Poulenc Rorer S.A. Purification d'adn plasmidique de qualite pharmaceutique
FR2776669A1 (fr) * 1998-03-24 1999-10-01 Rhone Poulenc Rorer Sa Vecteurs de transfert d'acides nucleiques, compositions les contenant, et leurs utilisations

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011390A1 (fr) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Detection de sequences d'acide nucleique par la formation d'une triple helice de l'adn
WO1996018744A2 (fr) * 1994-12-16 1996-06-20 Rhone-Poulenc Rorer S.A. Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
WO1996026270A1 (fr) * 1995-02-23 1996-08-29 Rhone-Poulenc Rorer S.A. Molecules d'adn, preparation et utilisation en therapie genique
WO1997035002A1 (fr) * 1996-03-21 1997-09-25 Rhone-Poulenc Rorer S.A. Purification d'adn plasmidique de qualite pharmaceutique
FR2776669A1 (fr) * 1998-03-24 1999-10-01 Rhone Poulenc Rorer Sa Vecteurs de transfert d'acides nucleiques, compositions les contenant, et leurs utilisations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GORSKI D H ET AL: "MOLECULAR CLONING OF A DIVERGED HOMEOBOX GENE THAT IS RAPIDLY DOWN-REGULATED DURING TH G0/G1 TRANSITION IN VASCULAR SMOOTH MUSCLE CELLS", MOLECULAR AND CELLULAR BIOLOGY, WASHINGTON, DC, US, vol. 13, no. 6, 1 June 1993 (1993-06-01), pages 3722 - 3733, XP000576016, ISSN: 0270-7306 *
JAYASENA S D ET AL: "OLIGONUCLEOTIDE-DIRECTED TRIPLE HELIX FORMATION AT ADJACENT OLIGOPURINE AND OLIGOPYRIMIDINE DNA TRACTS BY ALTERNATE STRAND RECOGNITION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 20, 1992, pages 5279 - 5288, XP002000132, ISSN: 0305-1048 *
SOYFER ET AL: "Triple Helical Nucleic Acids", 1996, SPRINGER, NEW YORK, XP001041881 *

Also Published As

Publication number Publication date
BR0208292A (pt) 2004-04-13
HUP0303632A3 (en) 2005-12-28
WO2002077274A2 (fr) 2002-10-03
MXPA03008642A (es) 2004-06-30
KR20030088464A (ko) 2003-11-19
JP4444564B2 (ja) 2010-03-31
JP2004523245A (ja) 2004-08-05
HUP0303632A2 (hu) 2004-01-28
NO20034184L (no) 2003-11-04
PL365208A1 (en) 2004-12-27
NO20034184D0 (no) 2003-09-19
IL158017A (en) 2011-07-31
KR101002499B1 (ko) 2010-12-17
CN1498276A (zh) 2004-05-19
AU2002253261C1 (en) 2002-10-08
KR20090094870A (ko) 2009-09-08
AU2002253261C9 (en) 2002-10-08
IL158017A0 (en) 2004-03-28
EP1370692A2 (fr) 2003-12-17
NZ528507A (en) 2005-08-26
CA2440133A1 (fr) 2002-10-03

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