JP2008509391A - テラヘルツ放射を用いて生体分子結合を検出するための方法及び系 - Google Patents
テラヘルツ放射を用いて生体分子結合を検出するための方法及び系 Download PDFInfo
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Abstract
【選択図】なし
Description
(1)Fab断片は、抗体分子の一価抗原結合断片から成り、酵素パパインで全抗体分子を消化して、無傷軽鎖及び重鎖の一部から成る断片を生じることにより産生され得る。
(2)抗体分子のFab’断片は、ペプシンで全抗体分子を処理し、その後還元して、無傷軽鎖及び重鎖の一部から成る分子を生じることにより生成され得る。2つのFab’断片は、この方法で処理された抗体分子当たりで得られる。
(3)抗体の(Fab’)2断片は、酵素ペプシンで全抗体分子を処理することにより、その後還元せずに得られる。(Fab’)2断片は、2つのジスルフィド結合により共に保持される2つのFab’断片の二量体である。
(4)Fv断片は、2つの鎖として発現される軽鎖の可変部及び重鎖の可変部を含有する遺伝子工学処理された断片と定義される。
(5)一本鎖抗体(「SCA」)は、適切な柔軟性ポリペプチドリンカーにより連結される軽鎖の可変部及び重鎖の可変部を含有する遺伝子工学処理された一本鎖分子である。
Claims (63)
- 生体分子相互作用を検出するための方法であって、以下の:
第1の分子と第2の分子を接触させること(ここで、該第1の分子は該第2の分子と結合すると思われる生体分子である)、
前記第1の分子にギガヘルツ又はテラヘルツ入射放射線を衝突させること、及び
出射放射線を検出すること(ここで、前記第2の分子と結合されない第1の分子から検出される出射放射線と比較した場合の振動周波数のシフト又は該出射放射線の強度レベルの変化は前記生体分子相互作用を示す)
を包含する方法。 - 前記第1の分子が基板上に固定される、請求項1記載の方法。
- 前記ギガヘルツ又はテラヘルツ放射線が前記基板を通して前記第1の分子に衝突する、請求項2記載の方法。
- 前記第1の分子が核酸である、請求項1記載の方法。
- 前記第2の分子が核酸である、請求項4記載の方法。
- 前記第1の分子及び/又は前記第2の分子がヒト核酸である、請求項5記載の方法。
- 前記第1の分子及び/又は前記第2の分子が細菌核酸である、請求項5記載の方法。
- 前記第1の分子及び/又は前記第2の分子がウイルス核酸である、請求項5記載の方法。
- 前記第2の分子がタンパク質である、請求項4記載の方法。
- 前記第1の分子が受容体であり、そして前記第2の分子がリガンドである、請求項1記載の方法。
- 前記第1の分子がタンパク質であり、そして前記第2の分子がタンパク質である、請求項1記載の方法。
- 強度レベル低減が検出される、請求項1記載の方法。
- 前記ギガヘルツ又はテラヘルツ放射線が前記第1の分子により吸収されることが既知の標的波長範囲を含む、請求項12記載の方法。
- 導波管の内壁に前記入射放射線を反射させることにより、該入射放射線が前記第1の分子に隣接して配置される前記導波管を通して向けられる、請求項1記載の方法。
- 前記入射放射線が0.1〜10テラヘルツの帯域幅を有する、請求項1記載の方法。
- 前記振動周波数のシフトがラマン分光分析を用いて検出することにより検出される、請求項1記載の方法。
- 生体分子を検出するための方法であって、以下の:
a)前記生体分子にギガヘルツ又はテラヘルツ入射放射線を衝突させること、及び
b)出射放射線を検出すること(ここで、前記入射放射線と比較した場合の該出射放射線の振動周波数のシフト、或いは前記分子を含まない対照試料から検出される出射放射線と比較した場合の該出射放射線の強度レベルの変化は前記分子の存在を示す)
を包含する方法。 - ラマン分光分析が前記出射放射線を検出するために用いられる、請求項A1記載の方法。
- 前記生体分子が少なくとも1キロダルトンの分子量を有する、請求項A1記載の方法。
- 前記生体分子が少なくとも10キロダルトンの分子量を有する、請求項A1記載の方法。
- 前記生体分子が核酸分子である、請求項17記載の方法。
- 前記生体分子がタンパク質である、請求項17記載の方法。
- 生物学的試料中の分析物を検出するための方法であって、以下の:
a)前記生物学的試料と基板上に固定された第1の分子とを接触させること(ここで、該第1の分子は前記分析物と結合すると思われる)、
b)前記第1の分子にギガヘルツ又はテラヘルツ入射放射線を衝突させること、及び
c)前記第1の分子の振動スペクトルを検出すること(ここで、前記分析物と関連しない第1の分子の振動スペクトルと比較した場合の該振動スペクトルのシフト又は該振動スペクトルの強度レベルの低減は前記試料中の前記分析物の存在を示す)
を包含する方法。 - 前記生物学的試料が血液、尿、唾液、組織、射精液、血清、脳脊髄液、胸水、腹水、痰、糞便又は生検試料からである、請求項23記載の方法。
- 生体分子結合に影響を及ぼす作用物質を同定するための方法であって、以下の:
a)第1の分子、第2の分子及び前記作用物質を接触させること(ここで、該第1の分子は該第2の分子と結合する)、
b)前記第1の分子にギガヘルツ又はテラヘルツ光源を衝突させること、及び
c)前記第1の分子の振動スペクトルを検出すること(ここで、前記作用物質の非存在下で前記第2の分子に結合される第1の分子の振動スペクトルと比較した場合の該振動スペクトルのシフト又は該振動スペクトルの強度レベルの変化は前記分子結合に影響を及ぼす前記作用物質を同定する)
を包含する方法。 - 前記第1の分子が核酸分子である、請求項25記載の方法。
- 前記第1の分子がタンパク質である、請求項25記載の方法。
- 前記タンパク質が抗体である、請求項27記載の方法。
- 生体分子結合事象を検出するための方法であって、以下の:
a)第1の分子及び第2の分子を含む試料を調製すること、
b)前記試料に入射放射線を向けて、それにより該入射放射線が蛍光を生じることなく前記試料を励起すること、及び
c)前記試料から移動する出射放射線を検出すること(ここで、前記第2の分子と結合されない第1の分子に関して検出される前記出射放射線と比較した場合の該出射放射線の振動周波数のシフト又は該出射放射線の強度レベルの変化は生体分子相互作用を示す)
を包含する方法。 - 生体分子結合事象を検出するための系であって、以下の:
a)第1の分子及び第2の分子を含有する試料(ここで、該第1の分子は該第2の分子と結合すると思われる)と、
b)入射放射線を前記試料に向けるよう配置されるテラヘルツ又はギガヘルツ放射線源と、
c)前記試料から反射する出射放射線を受信するための検出器と
を包含する系。 - 前記テラヘルツ放射線を通す基板をさらに包含する(ここで、前記第1の分子は前記基板上に固定される)、請求項30記載の系。
- 前記基板に隣接する反応小室をさらに包含する、請求項31記載の系。
- 前記入れ物中の溶液が前記第2の分子を含む、請求項32記載の系。
- 前記溶液が水を含む、請求項33記載の系。
- 前記基板が導波管の表面である、請求項31記載の系。
- 前記試料と前記検出器との間に配置されるわずか1つの回析格子をさらに含む、請求項30記載の系。
- 前記第1の分子が核酸である、請求項30記載の方法。
- 前記第2の分子が核酸である、請求項30記載の方法。
- 前記第2の分子がタンパク質である、請求項30記載の方法。
- 前記第1の分子が受容体であり、そして前記第2の分子がリガンドである、請求項30記載の方法。
- 前記第1の分子がタンパク質であり、そして前記第2の分子がタンパク質である、請求項30記載の方法。
- 第1の分子及び第2の分子を含有する試料中の生体分子結合事象を検出するための装置(ここで、前記第1の分子は前記第2の分子と結合すると思われる)であって、以下の:
入射放射線を前記試料に向けるよう配置されるテラヘルツ放射線源と、
前記試料から反射する出射放射線を受信するよう配置される検出器と
を包含する装置。 - 前記検出器と通信する情報処理及び制御系をさらに包含する、請求項42記載の装置。
- 前記情報処理及び制御系が前記検出器から受信されるデータを分析し得る、請求項43記載の装置。
- スペクトル分析器をさらに包含する、請求項42記載の装置。
- 試料ホルダーをさらに包含する、請求項42記載の装置。
- 前記試料ホルダーは前記テラヘルツ放射線を通す基板をさらに包含する(ここで、前記第1の分子は前記基板上に固定される)、請求項46記載の装置。
- 前記基板に隣接する反応小室をさらに包含する(ここで、該反応小室中の溶液が前記第2の分子を含む)、請求項47記載の装置。
- 前記基板が入力部分及び出力部分を有する導波管を含み、前記第1の分子が該導波管上に固定される、請求項47記載の装置。
- 前記テラヘルツ放射線源が前記導波管の入力部分に前記入射放射線を向けるよう配置され、そして前記検出器が該導波管の出力部分からの前記出射放射線を受信するよう配置される、請求項49記載の装置。
- 前記テラヘルツ放射線源により放出される所定の帯域幅の放射線を選択するためのフィルターをさらに包含する、請求項42記載の装置。
- 前記テラヘルツ放射線源が0.001〜1000THzの帯域幅を有する、請求項42記載の装置。
- 前記第1の分子が核酸である、請求項42記載の装置。
- 前記第2の分子が核酸である、請求項42記載の装置。
- 前記第2の分子がタンパク質である、請求項42記載の装置。
- 前記第1の分子が受容体であり、そして前記第2の分子がリガンドである、請求項42記載の装置。
- 前記第1の分子がタンパク質であり、そして前記第2の分子がタンパク質である、請求項42記載の装置。
- 第1の分子及び第2の分子を含有する試料中の生体分子結合事象を検出するための微小電気機械システム(MEMS)(ここで、前記第1の分子は前記第2の分子と結合すると思われる)であって、以下の:
前記試料のための1つ又は複数の流体区画と、
入射放射線を前記試料に向けるよう配置される放射線源と、
前記試料から反射する出射放射線を受信するよう配置される検出器と
を包含するシステム。 - 前記検出器と通信する情報処理及び制御系をさらに包含する、請求項58記載の装置。
- 前記流体区画は前記テラヘルツ放射線を通す基板をさらに包含する(ここで、前記第1の分子は前記基板上に固定される)、請求項59記載の装置。
- 前記基板が入力部分及び出力部分を有する導波管を含み、前記第1の分子が該導波管上に固定される、請求項60記載の装置。
- 前記放射線源が前記導波管の入力部分に前記入射放射線を向けるよう配置され、そして前記検出器が該導波管の出力部分からの前記出射放射線を受信するよう配置される、請求項61記載の装置。
- 前記放射線源により放出される所定の帯域幅の放射線を選択するためのフィルターをさらに包含する、請求項58記載の装置。
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2006
- 2006-05-30 US US11/443,639 patent/US20060216743A1/en not_active Abandoned
- 2006-05-30 US US11/443,633 patent/US20060216742A1/en not_active Abandoned
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2007
- 2007-01-12 GB GB0700616A patent/GB2433987B/en not_active Expired - Fee Related
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2010
- 2010-04-05 US US12/753,946 patent/US8852955B2/en not_active Expired - Fee Related
Patent Citations (1)
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US20040058339A1 (en) * | 2000-07-10 | 2004-03-25 | Michael Nagel | Method for detecting polynucleotide sequences |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006275592A (ja) * | 2005-03-28 | 2006-10-12 | Canon Inc | 検体保持用のデバイス、それを用いた検体検出装置及び検体検出方法 |
JP4636917B2 (ja) * | 2005-03-28 | 2011-02-23 | キヤノン株式会社 | 検体保持用のデバイス、それを用いた検体検出装置及び検体検出方法 |
JP2018004365A (ja) * | 2016-06-30 | 2018-01-11 | ローム株式会社 | 反射式検出装置 |
KR102090401B1 (ko) * | 2018-10-18 | 2020-03-17 | 부산대학교 산학협력단 | 분자 간 상호작용에 의한 흡광도 변화 예측 방법 |
WO2020080576A1 (ko) * | 2018-10-18 | 2020-04-23 | 연세대학교 산학협력단 | 분자 간 상호작용에 의한 흡광도 변화 예측 방법 |
Also Published As
Publication number | Publication date |
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JP4676983B2 (ja) | 2011-04-27 |
US20060216743A1 (en) | 2006-09-28 |
US20100278697A1 (en) | 2010-11-04 |
TW200609506A (en) | 2006-03-16 |
US20060029941A1 (en) | 2006-02-09 |
WO2006137824A2 (en) | 2006-12-28 |
WO2006137824A3 (en) | 2007-04-19 |
GB2433987A (en) | 2007-07-11 |
GB2433987B (en) | 2010-04-21 |
US7709247B2 (en) | 2010-05-04 |
GB0700616D0 (en) | 2007-02-21 |
DE112005001895T5 (de) | 2007-06-21 |
TWI303314B (en) | 2008-11-21 |
DE112005001895B4 (de) | 2011-12-08 |
US20060216742A1 (en) | 2006-09-28 |
US8852955B2 (en) | 2014-10-07 |
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