JP2008156299A - 脂肪分解促進用皮膚外用剤 - Google Patents
脂肪分解促進用皮膚外用剤 Download PDFInfo
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Abstract
【解決手段】ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする脂肪分解促進用皮膚外用剤。
【選択図】なし
Description
しかしながら、ラクトバチルス属に属する乳酸菌に脂肪分解促進作用があることはこれまでに知られていない。
1)ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする脂肪分解促進用皮膚外用剤。
2)ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする痩身用皮膚外用剤。
3)ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を皮膚に適用する痩身方法。
当該乳酸菌の菌体としては、菌体破壊物や乾燥菌体が挙げられる。
MRS寒天培地(Oxoid)で37℃、2日培養したLactobacillus helveticus SBT0064株の一白金耳を40mLのA培地(脱脂粉乳11.5%、酵母エキス0.5%)に接種し、37℃で3日静置培養した。培養液に40mLの酢酸エチルを添加して抽出した後、乾燥して抽出物の乾燥重量を測定した。また、他の7菌株に関しては、A培地またはMRS培地を用いて培養し、40mLの酢酸エチルを用いて抽出操作を行った。この時、未使用の培地からの抽出物を培地コントロールとした。表1に抽出物の一覧を示した。
ロッドベルの方法〔Rodbell,M.,J.Biol.Chem.,239(2)375-380(1964)〕に従って遊離脂肪細胞を調製した。ウイスター系ラット(雄、8週令)を日本SLC(株)より購入し、通常食(CE−2)にて飼育した。購入後2週間以内のラットを頚椎脱臼後、鼠径部組織からコラゲナーゼ溶液を用いて遊離細胞を調製した。被験物は、表1に示す抽出物をエタノールに溶解し、乾燥物換算で被験物濃度が10μg/mL及びノルエピネフリンが0.03μg/mLとなるように調製した牛血清アルブミンを含むハンクス緩衝液中で、上記細胞を37℃にて2時間インキュベートし、遊離したグリセロールを酵素法により定量した。対照は、被験物を含まないノルエピネフリンのみでインキュベートし、下記の式により脂肪分解促進活性を算出した。
Claims (3)
- ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする脂肪分解促進用皮膚外用剤。
- ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする痩身用皮膚外用剤。
- ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を皮膚に適用する痩身方法。
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JP2006348191A JP5201832B2 (ja) | 2006-12-25 | 2006-12-25 | 脂肪分解促進用皮膚外用剤 |
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JP2008156299A true JP2008156299A (ja) | 2008-07-10 |
JP5201832B2 JP5201832B2 (ja) | 2013-06-05 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010200611A (ja) * | 2009-02-27 | 2010-09-16 | Hiroshima Univ | 肥満予防又は改善剤 |
EP2210609A4 (en) * | 2007-10-29 | 2011-04-13 | Snow Brand Milk Products Co Ltd | AGENT PROMOTING ADIPONECTIN SECRETION AND / OR INHIBITING REDUCTION OF ADIPONECTIN SECRETION |
JP2012100655A (ja) * | 2010-10-15 | 2012-05-31 | National Agriculture & Food Research Organization | カテコールアミン認識性を指標に選抜した微生物とカテコールアミン含有組成物 |
JPWO2011155518A1 (ja) * | 2010-06-08 | 2013-08-01 | カルピス株式会社 | 脂質代謝改善剤 |
JP2017048244A (ja) * | 2016-12-01 | 2017-03-09 | 株式会社東洋新薬 | コラーゲン産生促進用組成物、コラーゲン吸収促進用組成物、抗肥満用組成物 |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000044482A (ja) * | 1998-07-30 | 2000-02-15 | Kao Corp | 脂肪分解促進剤 |
JP2001302523A (ja) * | 2000-04-27 | 2001-10-31 | Meiji Milk Prod Co Ltd | Ldl酸化抑制飲食品及び医薬品 |
JP2003306436A (ja) * | 2002-02-15 | 2003-10-28 | Snow Brand Milk Prod Co Ltd | 血清コレステロール上昇抑制剤 |
JP2004520276A (ja) * | 2000-11-10 | 2004-07-08 | プロビ エービー | 代謝症候群に関与する危険因子を減少するLactobacillus株の使用 |
JP2004307365A (ja) * | 2003-04-03 | 2004-11-04 | Kao Corp | 脂肪分解促進剤 |
JP2005052090A (ja) * | 2003-08-05 | 2005-03-03 | Kaneka Corp | 発酵乳製品およびそれを含有する食品 |
JP2005060366A (ja) * | 2003-07-29 | 2005-03-10 | Kao Corp | 脂肪分解促進剤 |
WO2006025643A1 (en) * | 2004-09-02 | 2006-03-09 | Pl Bio Co., Ltd. | Lactobacillus plantarum with body-fat reducing activity and the foods containing them |
JP2006182654A (ja) * | 2004-12-24 | 2006-07-13 | Toyo Shinyaku:Kk | 体脂肪の蓄積抑制または低減剤 |
JP2006265142A (ja) * | 2005-03-23 | 2006-10-05 | Toyo Shinyaku:Kk | 体脂肪の蓄積抑制または低減剤 |
-
2006
- 2006-12-25 JP JP2006348191A patent/JP5201832B2/ja active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000044482A (ja) * | 1998-07-30 | 2000-02-15 | Kao Corp | 脂肪分解促進剤 |
JP2001302523A (ja) * | 2000-04-27 | 2001-10-31 | Meiji Milk Prod Co Ltd | Ldl酸化抑制飲食品及び医薬品 |
JP2004520276A (ja) * | 2000-11-10 | 2004-07-08 | プロビ エービー | 代謝症候群に関与する危険因子を減少するLactobacillus株の使用 |
JP2003306436A (ja) * | 2002-02-15 | 2003-10-28 | Snow Brand Milk Prod Co Ltd | 血清コレステロール上昇抑制剤 |
JP2004307365A (ja) * | 2003-04-03 | 2004-11-04 | Kao Corp | 脂肪分解促進剤 |
JP2005060366A (ja) * | 2003-07-29 | 2005-03-10 | Kao Corp | 脂肪分解促進剤 |
JP2005052090A (ja) * | 2003-08-05 | 2005-03-03 | Kaneka Corp | 発酵乳製品およびそれを含有する食品 |
WO2006025643A1 (en) * | 2004-09-02 | 2006-03-09 | Pl Bio Co., Ltd. | Lactobacillus plantarum with body-fat reducing activity and the foods containing them |
JP2006182654A (ja) * | 2004-12-24 | 2006-07-13 | Toyo Shinyaku:Kk | 体脂肪の蓄積抑制または低減剤 |
JP2006265142A (ja) * | 2005-03-23 | 2006-10-05 | Toyo Shinyaku:Kk | 体脂肪の蓄積抑制または低減剤 |
Non-Patent Citations (1)
Title |
---|
JPN6012025924; 日高絢子, et al.: 'プロバイオティク系アシドフィラスグループ乳酸菌の脂肪分解特性' 日本畜産学会大会講演要旨 Vol. 104th, 20050320, p. 176 (IX27-7) * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2210609A4 (en) * | 2007-10-29 | 2011-04-13 | Snow Brand Milk Products Co Ltd | AGENT PROMOTING ADIPONECTIN SECRETION AND / OR INHIBITING REDUCTION OF ADIPONECTIN SECRETION |
US9387228B2 (en) | 2009-02-27 | 2016-07-12 | Hiroshima University | Agent for prevention or amelioration of obesity |
US20110311502A1 (en) * | 2009-02-27 | 2011-12-22 | Hiroshima University | Agent for prevention or amelioration of obesity |
EP2402431A1 (en) * | 2009-02-27 | 2012-01-04 | Hiroshima University | Agent for prevention or amelioration of obesity |
EP2402431A4 (en) * | 2009-02-27 | 2013-04-03 | Univ Hiroshima | AGENT FOR THE PREVENTION OR IMPROVEMENT OF OBESITY |
TWI461154B (zh) * | 2009-02-27 | 2014-11-21 | Univ Hiroshima | Obesity prevention or ameliorating agent |
JP2010200611A (ja) * | 2009-02-27 | 2010-09-16 | Hiroshima Univ | 肥満予防又は改善剤 |
JPWO2011155518A1 (ja) * | 2010-06-08 | 2013-08-01 | カルピス株式会社 | 脂質代謝改善剤 |
JP5801802B2 (ja) * | 2010-06-08 | 2015-10-28 | カルピス株式会社 | 脂質代謝改善剤 |
US9468657B2 (en) | 2010-06-08 | 2016-10-18 | Asahi Group Holdings, Ltd. | Lactic acid bacterium agent for improving lipid metabolism |
EP2581092B1 (en) | 2010-06-08 | 2017-04-05 | Asahi Group Holdings, Ltd. | Agent for improving lipid metabolism |
JP2012100655A (ja) * | 2010-10-15 | 2012-05-31 | National Agriculture & Food Research Organization | カテコールアミン認識性を指標に選抜した微生物とカテコールアミン含有組成物 |
JP2017048244A (ja) * | 2016-12-01 | 2017-03-09 | 株式会社東洋新薬 | コラーゲン産生促進用組成物、コラーゲン吸収促進用組成物、抗肥満用組成物 |
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