JP5201832B2 - 脂肪分解促進用皮膚外用剤 - Google Patents
脂肪分解促進用皮膚外用剤 Download PDFInfo
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Description
しかしながら、ラクトバチルス属に属する乳酸菌に脂肪分解促進作用があることはこれまでに知られていない。
1)ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする脂肪分解促進用皮膚外用剤。
2)ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を有効成分とする痩身用皮膚外用剤。
3)ラクトバチルス属に属する乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物を皮膚に適用する痩身方法。
当該乳酸菌の菌体としては、菌体破壊物や乾燥菌体が挙げられる。
MRS寒天培地(Oxoid)で37℃、2日培養したLactobacillus helveticus SBT0064株の一白金耳を40mLのA培地(脱脂粉乳11.5%、酵母エキス0.5%)に接種し、37℃で3日静置培養した。培養液に40mLの酢酸エチルを添加して抽出した後、乾燥して抽出物の乾燥重量を測定した。また、他の7菌株に関しては、A培地またはMRS培地を用いて培養し、40mLの酢酸エチルを用いて抽出操作を行った。この時、未使用の培地からの抽出物を培地コントロールとした。表1に抽出物の一覧を示した。
ロッドベルの方法〔Rodbell,M.,J.Biol.Chem.,239(2)375-380(1964)〕に従って遊離脂肪細胞を調製した。ウイスター系ラット(雄、8週令)を日本SLC(株)より購入し、通常食(CE−2)にて飼育した。購入後2週間以内のラットを頚椎脱臼後、鼠径部組織からコラゲナーゼ溶液を用いて遊離細胞を調製した。被験物は、表1に示す抽出物をエタノールに溶解し、乾燥物換算で被験物濃度が10μg/mL及びノルエピネフリンが0.03μg/mLとなるように調製した牛血清アルブミンを含むハンクス緩衝液中で、上記細胞を37℃にて2時間インキュベートし、遊離したグリセロールを酵素法により定量した。対照は、被験物を含まないノルエピネフリンのみでインキュベートし、下記の式により脂肪分解促進活性を算出した。
Claims (5)
- ラクトバチルス・ヘルベティクス、ラクトバチルス・デルブルッキー、ラクトバチルス・ガセリ及びラクトバチルス・プランタラムからなる群より選択される乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物(但し、当該乳酸菌を用いてトウガラシを発酵処理して得られる発酵物を除く)を有効成分とする脂肪分解促進用皮膚外用剤。
- ラクトバチルス・ヘルベティクス、ラクトバチルス・デルブルッキー、ラクトバチルス・ガセリ及びラクトバチルス・プランタラムからなる群より選択される乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物(但し、当該乳酸菌を用いてトウガラシを発酵処理して得られる発酵物を除く)を有効成分とする痩身用皮膚外用剤。
- 前記抽出物がエステル又はアルコール抽出物である請求項1又は2記載の剤。
- ラクトバチルス・ヘルベティクス、ラクトバチルス・デルブルッキー、ラクトバチルス・ガセリ及びラクトバチルス・プランタラムからなる群より選択される乳酸菌の菌体若しくは菌体培養物又はこれらの抽出物(但し、当該乳酸菌を用いてトウガラシを発酵処理して得られる発酵物を除く)を皮膚に適用する、医療行為を含まない美容のための痩身方法。
- 前記抽出物がエステル又はアルコール抽出物である請求項4記載の方法。
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