JP2006204252A - Method for breeding ligneous plant - Google Patents

Method for breeding ligneous plant Download PDF

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JP2006204252A
JP2006204252A JP2005023644A JP2005023644A JP2006204252A JP 2006204252 A JP2006204252 A JP 2006204252A JP 2005023644 A JP2005023644 A JP 2005023644A JP 2005023644 A JP2005023644 A JP 2005023644A JP 2006204252 A JP2006204252 A JP 2006204252A
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breeding
mother tree
mother
cultivating
leaves
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Yukimasa Hirata
行正 平田
Kenshiro Kosaka
憲史郎 小坂
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WAKAYAMAKEN NOGYO KYODO KUMIAI
WAKAYAMAKEN NOGYO KYODO KUMIAI RENGOKAI
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WAKAYAMAKEN NOGYO KYODO KUMIAI
WAKAYAMAKEN NOGYO KYODO KUMIAI RENGOKAI
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for breeding a ligneous plant, which is readily put to practical use since scions having a high rooting ratio necessary for breeding a ligneous plant by a green wood cutting are mass-produced by reduced labor without much damaging a mother tree. <P>SOLUTION: The method for breeding a ligneous plant comprises cultivating a bare mother tree in a low illumination environment to produce leaves and using green wood having the leaves as a scion. The low illumination intensity has 1-20,000 luxes, preferably 1,000-5,000 luxes represented by the maximum illumination intensity in a cultivation period and about 50-3,000 luxes represented by an average illumination intensity in the period. The cultivation in a low illumination environment means concretely cultivating a mother tree in a pot placed in a dark place, covering a glass greenhouse for cultivating mother trees with a light shielding material such as a cheesecloth, etc., and cultivating the mother trees or covering a mother tree itself with a light shielding material and cultivating the mother tree. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

この発明は、カキをはじめとする木本性植物の繁殖方法に関し、特に木本性植物の緑枝挿しによる繁殖方法に関する。   The present invention relates to a method for breeding woody plants such as oysters, and more particularly to a method for breeding woody plants by inserting green branches.

挿し木法は、植物の増殖方法の分野では無性繁殖法に属し、種子による繁殖法である有性繁殖法とは異なり、母樹から切り離した緑枝、休眠枝、熟枝などの挿し穂を、挿し床に挿して発根させ苗木となるまで栽培するものである。そのため、母樹と同じ形質(特徴)の植物体を繁殖できるという利点を備えており、挿し木法は、優良な植物個体から優良な苗木を大量に生産する技術として一般に行われている。なお、挿し木法により生産された苗木は、種子から生産された苗木と同様、果樹園や山林などに移植され、果実や木材の生産、環境の保護などに供されている。   The cutting method belongs to the asexual breeding method in the field of plant propagation methods, and unlike the sexual breeding method which is a seed breeding method, cuttings such as green branches, dormant branches and mature branches separated from the mother tree, It is cultivated until it is rooted by inserting it into the cutting floor until it becomes a seedling. Therefore, it has an advantage that a plant body having the same character (characteristic) as that of the mother tree can be propagated, and the cutting method is generally performed as a technique for producing a large amount of excellent seedlings from an excellent plant individual. In addition, the seedlings produced by the cutting method are transplanted to orchards, mountain forests, etc., as well as seedlings produced from seeds, and are used for the production of fruits and timber, environmental protection, and the like.

さて、上記のように挿し穂を挿し床に挿した際に、挿し穂が発根する割合(以下、発根率という。)は、木本性植物ではあまり高くなかった。そのため、従来から発根率を高くする挿し木法の開発が望まれており、様々な研究が行われてきている。このような研究としては、例えば、ひこばえや黄化処理を使用する繁殖方法(非特許文献1及び非特許文献2を参照。)、挿し穂の表皮を削ぐ方法(特許文献1を参照。)、挿し穂の切り口を薬品などにより処理する方法(特許文献2を参照。)等が挙げられる。   Now, when the cuttings are inserted into the floor as described above, the ratio of the cuttings to root (hereinafter referred to as rooting rate) was not so high in woody plants. Therefore, it has been desired to develop a cutting method for increasing the rooting rate, and various studies have been conducted. As such research, for example, a breeding method using hibobae or yellowing treatment (see Non-Patent Document 1 and Non-Patent Document 2), a method of cutting the epidermis of the cutting ear (see Patent Document 1) ), A method of treating the cut end of the cutting head with a chemical or the like (see Patent Document 2), and the like.

ここで、ひこばえを使用する繁殖方法とは、母樹の地上部を切り倒して、その切り株の周囲に籾殻などを散布したのち、切り株の周りに張った根から生える若芽(ひこばえ)をそのまま挿し穂として使用する方法である。また、黄化処理を使用する繁殖方法とは、母樹を一定期間光が当たらない環境(暗黒下)で栽培することにより、黄白色の葉枝(シュート)を発生させ、この黄化したシュートの基部を黒布、黒紙、アルミホイルなどの遮光材で覆ったのち、挿し穂を覆う遮光材を少しずつ剥きながら基部以外を緑化させ、これを母樹から切り離して挿し穂として挿し床に挿すことにより、挿し穂の発根を促す方法である。さらに、挿し穂の表皮を削ぐ方法とは、挿し穂として切り取られた枝の切断面近傍の表皮を削ぐことにより挿し穂の発根を促す方法であり、挿し穂の切り口を薬品などにより処理する方法とは、挿し穂の切り口にキトサンなどの多糖類やインドール酢酸などの植物ホルモンを浸すことにより挿し穂の発根を促す方法である。   Here, the breeding method using Hikobae is to cut down the above-ground part of the mother tree, spray rice husk around the stump, and then grow the young shoots (Hikobae) that grow from the roots around the stump. It is a method of using it as an insertion head as it is. In addition, the breeding method using yellowing treatment is to grow yellow-white leaves (shoots) by cultivating the mother tree in an environment where it is not exposed to light for a certain period of time (under the dark), and the base of this yellowed shoot Is covered with a shading material such as black cloth, black paper, aluminum foil, etc., and then the shading material covering the cutting head is peeled off little by little, and the parts other than the base are greened. It is a method to promote rooting of cuttings. Furthermore, the method of removing the epidermis of the cutting ear is a method of promoting rooting of the cutting ear by cutting the epidermis near the cut surface of the branch cut as the cutting ear, and the cutting end of the cutting ear is treated with a chemical or the like. The method is a method of promoting rooting of cuttings by immersing a polysaccharide such as chitosan or a plant hormone such as indoleacetic acid at the cut end.

しかし、これらの繁殖方法には次に掲げるような問題点があった。まず、ひこばえを使用する繁殖方法においては、繁殖する際に母樹を切断するため、その母樹からは切断した後は果実が取れなくなってしまうとの問題点があった。また、いずれの繁殖方法においても、母樹の切り倒しや籾殻の散布、遮光材による葉枝の被覆やその除去、表皮の削除、薬品処理等に多大な労力を必要とするため、実用化が難しいとの問題点があった。
特開平11−69912号公報 特開2002−34328公報 鉄村ら、“カキ休眠枝挿し穂の発根に及ぼす要因について”、園芸学会雑誌、70(2)、2001、p163−169 鉄村ら、“カキ緑眠枝挿し穂の発根に及ぼす要因について”、園芸学会雑誌、70(3)、2001、p275−280
However, these breeding methods have the following problems. First of all, in the breeding method using Hikobae, the mother tree is cut when breeding, so that there is a problem that the fruit cannot be removed from the mother tree after cutting. Also, in any breeding method, it is difficult to put it into practical use because it requires a great deal of effort to cut down the mother tree, spray rice husks, cover and remove leaf branches with a light shielding material, delete the epidermis, and chemical treatment. There was a problem.
JP-A-11-69912 JP 2002-34328 A Tetsumura et al., “About the Factors Affecting the Rooting of Oyster Dormant Branch Cuttings”, Horticultural Society Journal, 70 (2), 2001, p163-169. Tetsumura et al., “About the Factors Affecting the Rooting of Oyster Green Sleeping Branches,” Horticultural Society Journal, 70 (3), 2001, p275-280.

そこで、この発明は、母樹をあまり傷つけることなく、黄化(暗黒)処理など煩雑な前処理を行うことなく、植物に緑枝挿しによる木本性植物の繁殖に必要な発根率の高い挿し穂を少ない労力で量産することができ、実用化し易い木本性植物の繁殖方法を提供すること、を課題とする。   Therefore, the present invention has a high rooting rate necessary for breeding woody plants by inserting green branches into a plant without damaging the mother tree so much and without performing complicated pretreatment such as yellowing (darkness) treatment. It is an object of the present invention to provide a method for breeding woody plants that can be mass-produced with less labor and that is easy to put into practical use.

この発明の木本性植物の繁殖方法は、葉が生えることができる最小限の量に光の量(照度)を制限した環境下で母樹を栽培して葉を発生させ、この葉を含む緑枝を挿し穂として使用することを最大の特徴とする。   According to the method for breeding woody plants of the present invention, a mother tree is cultivated in an environment where the amount of light (illuminance) is limited to the minimum amount that leaves can grow, and leaves are generated. It is the biggest feature to use as an ear.

この発明の木本性植物の繁殖方法により、発根率の高い挿し穂が少ない労力、すなわち低コストで量産できるようになった。そのため、優れた性質を持った木本性植物を選抜して繁殖することができるようになり、例えば、品質のよい果実や疾病に強い果実を量産できるようになった。   According to the method for breeding woody plants of the present invention, mass production can be achieved at low cost, with a low rooting rate and with a low amount of cuttings. Therefore, woody plants having excellent properties can be selected and propagated, and for example, high-quality fruits and fruits resistant to diseases can be mass-produced.

この発明の木本性植物の繁殖方法は、落葉した母樹を低照度環境下で栽培して葉を生じさせ、この葉を含む緑枝を挿し穂として使用する。そこで、以下にこの内容について詳説する。   According to the method for breeding a woody plant of the present invention, a fallen mother tree is cultivated in a low light environment to produce leaves, and a green branch containing these leaves is used as an ear. Therefore, this content will be described in detail below.

(この発明の繁殖方法が適用可能な本木性植物)
前記木本性植物としては、カキ、ウメ、カンキツ、カラタチ、クリ、クルミ、ヤマモモなどが例示でき、なかでも商業的価値や他の方法での繁殖が困難であることからカキが好ましい。また、落葉した母樹としては、自然に落葉したものや、人手によって葉を取り除いたものなどが例示できるが、作業コストを考えると自然に落葉したものが好ましい。
(A true woody plant to which the breeding method of the present invention is applicable)
Examples of the woody plant include oysters, ume, citrus, scallops, chestnuts, walnuts, and bayberry, among which oysters are preferred because of their commercial value and difficulty in breeding by other methods. Further, examples of the fallen mother tree include naturally fallen leaves and those obtained by manually removing leaves, but naturally fallen leaves are preferable in view of work costs.

(低照度下での栽培)
前記の母樹をその幹や枝の芽が萌芽しかけてから薄黄緑色の葉が2枚〜20枚程度展開するまで、約10日〜約90日程度低照度環境下で栽培する。ここで、低照度環境とは、最大照度で表せば1〜20000ルクス、好ましくは1000〜5000ルクスの範囲内の環境であり、明期の平均照度で表せば50〜3000ルクス程度の環境である。なお、上記照度の値はルクス計などによって、常識的な日照時間(8時間から16時間程度)の間に、定法に従って測定したものである。また、低照度環境下での栽培とは、具体的には、暗所に置いた植木鉢で母樹を栽培したり、母樹を栽培しているガラスハウス等を寒冷紗等の遮光材で覆って栽培したり、母樹そのものを遮光材で覆って栽培することをいう。さらに、母樹を栽培する際の温度、湿度などについては、木本性植物を栽培する際の通常の温度、湿度であれば特に問題はないが、温度は10〜40℃が好ましく、湿度は飽和水蒸気量に対して10〜90%程度が好ましい。
(Cultivation under low illumination)
The mother tree is cultivated in a low light environment for about 10 days to about 90 days from the time when the stems and branches buds sprout until about 2 to 20 light yellow-green leaves develop. Here, the low illuminance environment is an environment within the range of 1 to 20000 lux, preferably 1000 to 5000 lux when expressed in terms of maximum illuminance, and is an environment of about 50 to 3000 lux when expressed as the average illuminance during the light period. . The illuminance value is measured by a lux meter or the like during a common-sense sunshine time (about 8 to 16 hours) according to a conventional method. In addition, cultivation under a low-light environment specifically refers to cultivation of a mother tree in a flowerpot placed in a dark place, or covering a glass house or the like where a mother tree is cultivated with a light-shielding material such as a cold chill. Or to cover the mother tree itself with a shading material. Furthermore, there are no particular problems regarding the temperature, humidity, etc. when cultivating the mother tree as long as it is a normal temperature, humidity when cultivating woody plants, but the temperature is preferably 10-40 ° C., and the humidity is saturated water vapor. About 10-90% is preferable with respect to quantity.

(緑枝の切断)
前記葉を含む緑枝を母樹から、鎌や鉈などの通常の農作業具やナイフ等により切断する。緑枝基部の切断形態としては、枝長手方向とほぼ直角に切断する「水平切り」、枝長手方向に対し斜めに切断する「斜め切り」、斜め切りした枝の切断先端を斜めに切り返す「斜め切返し」、切断部分の形状を下細の三角錐形状に切断する「三角切り」、水平切りした切断面に枝長手方向の割り込みを入れた「割り挿し」、割り挿しの割り込み部分に小枝などを差し込んで二股状にした「はさみ挿し」などが知られている(花卉園芸ハンドブック、養賢堂、第192ページ)が、木本性植物の種類に応じて好適な切断形態を採用すればよい。また、挿し穂を切る場所は、茎の先から8〜40cmの場所で、葉が1〜5枚ついた枝を調製できる場所がよい。
(Cutting green branches)
The green branch including the leaves is cut from the mother tree with a normal farming tool such as a sickle or a scissors or a knife. The cutting form of the green branch base is “horizontal cutting” that cuts substantially perpendicular to the longitudinal direction of the branch, “oblique cutting” that cuts diagonally with respect to the longitudinal direction of the branch, and “oblique cutting” that cuts the cutting tip of the obliquely cut branch diagonally. , "Triangular cut" that cuts the shape of the cut part into a lower triangular pyramid shape, "Interleave" with the interrupt in the longitudinal direction of the branch cut into the horizontal cut surface, and twigs etc. inserted into the interrupted interrupt part A bifurcated “scissor insertion” or the like is known (Flower Gardening Handbook, Yokendo, page 192), but a suitable cutting form may be adopted depending on the type of woody plant. Moreover, the place which cuts the cutting head is 8 to 40 cm from the tip of the stem, and a place where a branch with 1 to 5 leaves can be prepared.

(苗木の作出)
さらに、母樹から切断した緑枝葉を挿し床に挿して発根させ、苗木として使用可能な大きさになるまで約7日〜約60日程度栽培する。ここで、栽培に使用する挿し床としては、特に限定されないが、オアシス(商標、SMITHERS−OASIS社製の挿し木用培地、製品形式P1)、ロックウール、ソイルブロック、赤土、赤玉土、鹿沼土、ピートモス、ミズゴケ、バーク、バーミキュライト、パーライトなどを、単独であるいは複数種を適宜併用することができる。また、栽培に際しては、緑枝が蒸散作用などにより水分を多量に消費するので、30〜90%遮光し、葉水、ミスト、フォグなどにより、挿し穂が乾燥しないようにして栽培する必要があり、挿し穂の生育を促進するため、炭酸ガスの濃度が500〜4000ppmの環境で栽培するのが好ましい。
(Production of seedlings)
Furthermore, the green branches and leaves cut from the mother tree are inserted, rooted on the floor, and cultivated for about 7 days to about 60 days until it becomes a size that can be used as a seedling. Here, as an insertion floor used for cultivation, although not particularly limited, Oasis (trademark, medium for cuttings manufactured by Smithers-OASIS, product format P1), rock wool, soil block, red soil, red ball soil, Kanuma soil, Peat moss, sphagnum, bark, vermiculite, pearlite, and the like can be used alone or in appropriate combinations of a plurality of types. In addition, since green branches consume a large amount of water due to transpiration, etc., it is necessary to cultivate so that the cuttings do not dry out with leaf water, mist, fog, etc. In order to promote the growth of cuttings, it is preferable to grow in an environment where the concentration of carbon dioxide gas is 500 to 4000 ppm.

(苗木の栽培)
このようにして作出した苗木は、通常の方法により屋外圃場、ガラスハウス、山林などに移植して、果樹の生産、環境の保護の用途に使用することができる。
(Cultivation of seedlings)
The seedlings thus produced can be transplanted to outdoor fields, glass houses, mountain forests, etc. by ordinary methods and used for fruit tree production and environmental protection.

上記のように、この発明にかかる木本性植物の繁殖方法は、母樹を暗所に置いた植木鉢で栽培したり、母樹を寒冷紗で覆ったガラスハウス内で栽培したりするなど、母樹を低照度環境下で栽培するだけで、言い換えるならば、多大な労力を必要とせずに、発根率の高い挿し穂を得ることができる。   As described above, the method for breeding woody plants according to the present invention is such that the mother tree is cultivated in a flower pot placed in a dark place, or the mother tree is cultivated in a glass house covered with cold chill. In other words, it is possible to obtain cuttings with a high rooting rate without requiring a great deal of labor simply by cultivating under the environment.

なお、この発明にかかる木本性植物の繁殖方法は、上記の手順に加えて、従来からある発根促進処理を併用することも可能である。例えば、前記特許文献1に記載しているように、切断面近傍の表皮を削いだものを挿し穂として利用してもよい。また、10-9M〜10-2M濃度の発根促進ホルモン(例えば、オーキシンなど)や発根促進物質(例えば、エスレル,メネデール(商標、株式会社メネデール化学研究所製)など)を挿し穂あるいは挿し床に施用してもよい。 In addition, in addition to said procedure, the breeding method of the woody plant concerning this invention can also use conventional rooting promotion processing together. For example, as described in Patent Document 1, a material obtained by shaving the skin near the cut surface may be used as an insertion ear. In addition, a root-promoting hormone (for example, auxin) or a root-promoting substance (for example, Esler, Menedale (trademark, manufactured by Menedal Chemical Laboratory, Inc.)) having a concentration of 10 −9 M to 10 −2 M is inserted. Or you may apply to an insertion floor.

以下に、この発明を実施例に従ってさらに詳しく説明するが、この発明の特許請求の範囲は如何なる意味においても下記の実施例により限定されるものではない。   Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the claims of the present invention is not limited by the following examples in any way.

母樹である落葉したカキ(品種:富有、長さ約2m)を植えた植木鉢を、和歌山県農業協同組合連合会 営農対策部 植物バイオセンターの玄関(以下、実験区:玄関)、50%寒冷紗で二重被覆したプラスティックフィルムハウス(以下、実験区:ハウス)、及び遮光していないガラスハウス(以下、実験区:実験対照)に配置し、2004年4月2日から2004年6月1日までの60日間栽培した。なお、栽培期間中の最大照度及び明期の平均照度を下記の表1に示す。なお、表1に示す照度は、ルクス計(東京光電株式会社、ポケット照度計ANA−500型)により測定した。また、表1の照度欄にある「最大」とは栽培期間中の最大照度を示し、「平均」とは栽培期の明期の平均照度を示している。   A plant pot planted with a deciduous oyster (variety: Fuyu, about 2m in length), which is the mother tree, is the entrance of the Plant Biocenter, Wakayama Prefectural Agricultural Cooperative Federation (hereinafter referred to as “Experimental Zone: Entrance”), 50% cold chill Placed in a double-coated plastic film house (hereinafter referred to as experimental group: house) and a non-light-shielded glass house (hereinafter referred to as experimental group: experimental control) from April 2, 2004 to June 1, 2004 For 60 days. The maximum illuminance during the cultivation period and the average illuminance during the light period are shown in Table 1 below. In addition, the illuminance shown in Table 1 was measured with a lux meter (Tokyo Kogyo Co., Ltd., pocket illuminometer ANA-500 type). “Maximum” in the illuminance column of Table 1 indicates the maximum illuminance during the cultivation period, and “average” indicates the average illuminance during the light period of the cultivation period.

60日後、母樹から黄緑色の葉が生えた緑枝を葉が2枚付いた状態で基部を水平切りにより切断し、パーライト:バーミキュライト(1:1)を入れた育苗箱に緑枝挿しして、2004年6月1日から2004年7月12日までの42日間、50%遮光のミスト付きハウスで栽培し、その発根率を調べた。その結果を表1に示す。   After 60 days, cut the base of the green branch with yellow-green leaves from the mother tree with two leaves attached, and cut the base into a nursery box containing perlite: vermiculite (1: 1). Cultivated in a house with a 50% light-shielded mist for 42 days from June 1, 2004 to July 12, 2004, and the rooting rate was examined. The results are shown in Table 1.

Figure 2006204252
Figure 2006204252

この表1からも明らかなように、高照度下で栽培した母樹(実験区:実験対照)から得られた挿し穂の発根率が0%であるのに対して、これよりも低照度下で栽培した母樹(実験区:ハウス)やさらに低照度下で栽培した母樹(実験区:玄関)から得られた挿し穂の発根率は、75〜95%と充分実用性のある値であった。しかも、この高い発根率は、母樹を低照度環境下で栽培することを除けば、通常の挿し木法とほぼ同様の労力だけで得ることができた。   As apparent from Table 1, the rooting rate of the cuttings obtained from the mother tree (experimental section: experimental control) cultivated under high illuminance was 0%, but under low illuminance. The rooting rate of the cuttings obtained from the mother tree cultivated in (experimental section: house) and the parent tree cultivated under low illumination (experimental section: entrance) was 75-95%, which is a sufficiently practical value. It was. Moreover, this high rooting rate could be obtained with almost the same effort as the normal cutting method except that the mother tree was cultivated in a low light environment.

Claims (5)

落葉した母樹を低照度環境下で栽培して葉を生じさせ、この葉を含む緑枝を挿し穂として使用する木本性植物の繁殖方法。   A method for breeding woody plants in which a fallen mother tree is cultivated in a low-light environment to produce leaves, and green branches containing these leaves are used as ears. 落葉した母樹を最大照度が1ルクスから20000ルクスの環境下で栽培して葉を生じさせる請求項1に記載の木本性植物の繁殖方法。   The method for breeding woody plants according to claim 1, wherein the fallen mother tree is cultivated in an environment having a maximum illuminance of 1 lux to 20000 lux to produce leaves. 落葉した母樹を最大照度が1000ルクスから5000ルクスの環境下で栽培して葉を生じさせる請求項1に記載の木本性植物の繁殖方法。   The method for breeding woody plants according to claim 1, wherein the fallen mother tree is cultivated in an environment having a maximum illuminance of 1000 lux to 5000 lux to produce leaves. 落葉した母樹を明期の平均照度が50ルクスから3000ルクスの環境下で栽培して葉を生じさせる請求項1に記載の木本性植物の繁殖方法。   The method for breeding a woody plant according to claim 1, wherein the fallen mother tree is cultivated in an environment having an average light intensity of 50 lux to 3000 lux in the light period to produce leaves. 木本性植物がカキである請求項1から請求項4のいずれかに記載の木本性植物の繁殖方法。   The method for breeding woody plants according to any one of claims 1 to 4, wherein the woody plants are oysters.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103120091A (en) * 2013-02-26 2013-05-29 濮阳市林业科学院 Persimmon tree quick seedling culture method
JP2013132257A (en) * 2011-12-27 2013-07-08 Kyosen Sangyo Kk Method of cultivating black ginger
CN111034524A (en) * 2019-12-18 2020-04-21 桐乡市石门镇墅丰股份经济合作社 Planting method of Japanese persimmon

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013132257A (en) * 2011-12-27 2013-07-08 Kyosen Sangyo Kk Method of cultivating black ginger
CN103120091A (en) * 2013-02-26 2013-05-29 濮阳市林业科学院 Persimmon tree quick seedling culture method
CN111034524A (en) * 2019-12-18 2020-04-21 桐乡市石门镇墅丰股份经济合作社 Planting method of Japanese persimmon

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