JP2005521422A5 - - Google Patents
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- Publication number
- JP2005521422A5 JP2005521422A5 JP2003582312A JP2003582312A JP2005521422A5 JP 2005521422 A5 JP2005521422 A5 JP 2005521422A5 JP 2003582312 A JP2003582312 A JP 2003582312A JP 2003582312 A JP2003582312 A JP 2003582312A JP 2005521422 A5 JP2005521422 A5 JP 2005521422A5
- Authority
- JP
- Japan
- Prior art keywords
- dctp
- dgtp
- primer
- fluorescein
- incorporated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 6
- 229920001519 homopolymer Polymers 0.000 description 6
- 229920000272 Oligonucleotide Polymers 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 4
- HAAZLUGHYHWQIW-KVQBGUIXSA-N Deoxyguanosine triphosphate Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 101700011961 DPOM Proteins 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-N Deoxycytidine triphosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-N 0.000 description 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N Iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 description 1
- 101710029649 MDV043 Proteins 0.000 description 1
- 101700061424 POLB Proteins 0.000 description 1
- 101700054624 RF1 Proteins 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000000171 quenching Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
Description
（実施例1）
隣接して取り込まれたCy5−SS−dCTPを未熟切断から保護するためのチオール基のキャッピング
5'末端にビオチンを結合したオリゴヌクレオチド鋳型E3PN10b（配列番号４）及びE3PN22b（配列番号５）をプライマー（下記のNUSPT−フルオレッセイン（配列番号３））にアニールした。取り込むべき塩基を太字で示し、これには、下線付き太字で示したC取り込みの2箇所の位置が含まれる。
隣接して取り込まれたCy5−SS−dCTPを未熟切断から保護するためのチオール基のキャッピング
5'末端にビオチンを結合したオリゴヌクレオチド鋳型E3PN10b（配列番号４）及びE3PN22b（配列番号５）をプライマー（下記のNUSPT−フルオレッセイン（配列番号３））にアニールした。取り込むべき塩基を太字で示し、これには、下線付き太字で示したC取り込みの2箇所の位置が含まれる。
（実施例２）
Cy5‐SS‐dCTP/dCTP混合物を使用するホモポリマー的伸長における蛍光シグナルと取り込んだ塩基の数の間の直線関係の達成
5'末端にビオチンを結合したオリゴヌクレオチド鋳型をプライマー（上記NUSPT-フルオレッセイン）にアニールした。取り込むべき塩基を太字で示し、これには、下線付き太字で示したC取り込み位置が含まれる。
Cy5‐SS‐dCTP/dCTP混合物を使用するホモポリマー的伸長における蛍光シグナルと取り込んだ塩基の数の間の直線関係の達成
5'末端にビオチンを結合したオリゴヌクレオチド鋳型をプライマー（上記NUSPT-フルオレッセイン）にアニールした。取り込むべき塩基を太字で示し、これには、下線付き太字で示したC取り込み位置が含まれる。
（実施例３）
例：「指定分配」を使用する、オリゴヌクレオチドE3PN19bの塩基配列決定
例：「指定分配」を使用する、オリゴヌクレオチドE3PN19bの塩基配列決定
（実施例４）
標識/非標識ヌクレオチドに対するクレノーエキソDNAポリメラーゼの選択性の測定
標識/非標識ヌクレオチドに対するクレノーエキソDNAポリメラーゼの選択性の測定
（実施例５）
WO 00/53812によるCy5‐SS‐dGTP/dGTP混合物を使用してホモポリマー伸長に取り込まれた塩基の数と蛍光シグナルの間の関係の測定
WO 00/53812によるCy5‐SS‐dGTP/dGTP混合物を使用してホモポリマー伸長に取り込まれた塩基の数と蛍光シグナルの間の関係の測定
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36959902P | 2002-04-04 | 2002-04-04 | |
| SE0201024A SE0201024D0 (sv) | 2002-04-04 | 2002-04-04 | New method |
| PCT/SE2003/000547 WO2003085135A1 (en) | 2002-04-04 | 2003-04-04 | New method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005521422A JP2005521422A (ja) | 2005-07-21 |
| JP2005521422A5 true JP2005521422A5 (ja) | 2006-01-05 |
| JP4230364B2 JP4230364B2 (ja) | 2009-02-25 |
Family
ID=28793693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003582312A Expired - Fee Related JP4230364B2 (ja) | 2002-04-04 | 2003-04-04 | 新しい方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20050244827A1 (ja) |
| EP (1) | EP1495137B1 (ja) |
| JP (1) | JP4230364B2 (ja) |
| AT (1) | ATE304610T1 (ja) |
| AU (1) | AU2003214765B2 (ja) |
| CA (1) | CA2481495C (ja) |
| DE (1) | DE60301622T2 (ja) |
| WO (1) | WO2003085135A1 (ja) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002029003A2 (en) | 2000-10-06 | 2002-04-11 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding dna and rna |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| GB0129012D0 (en) | 2001-12-04 | 2002-01-23 | Solexa Ltd | Labelled nucleotides |
| US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
| SI3002289T1 (en) | 2002-08-23 | 2018-07-31 | Illumina Cambridge Limited | MODIFIED NUCLEOTES FOR POLYUCULOTIDE SEQUENCING |
| US11008359B2 (en) | 2002-08-23 | 2021-05-18 | Illumina Cambridge Limited | Labelled nucleotides |
| WO2004069849A2 (en) * | 2003-01-29 | 2004-08-19 | 454 Corporation | Bead emulsion nucleic acid amplification |
| WO2005060345A2 (en) * | 2003-12-22 | 2005-07-07 | Biotage Ab | New method for sequencing-by-synthesis |
| WO2008042067A2 (en) | 2006-09-28 | 2008-04-10 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| US7883869B2 (en) | 2006-12-01 | 2011-02-08 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| EP2207900B1 (en) | 2007-10-19 | 2015-04-29 | The Trustees of Columbia University in the City of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
| EP3431615A3 (en) | 2007-10-19 | 2019-02-20 | The Trustees of Columbia University in the City of New York | Dna sequencing with non-fluorescent nucleotide reversible terminators cleavable label modified nucleotide terminators |
| JP5431352B2 (ja) | 2007-11-28 | 2014-03-05 | ビーエーエスエフ ソシエタス・ヨーロピア | 液体安定剤混合物 |
| AU2009212165A1 (en) * | 2008-02-07 | 2009-08-13 | Pacific Biosciences Of California, Inc. | CIS reactive oxygen quenchers integrated into linkers |
| US8603792B2 (en) | 2009-03-27 | 2013-12-10 | Life Technologies Corporation | Conjugates of biomolecules to nanoparticles |
| US9146248B2 (en) | 2013-03-14 | 2015-09-29 | Intelligent Bio-Systems, Inc. | Apparatus and methods for purging flow cells in nucleic acid sequencing instruments |
| US9591268B2 (en) | 2013-03-15 | 2017-03-07 | Qiagen Waltham, Inc. | Flow cell alignment methods and systems |
| US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| JP6333297B2 (ja) | 2013-03-15 | 2018-05-30 | イルミナ ケンブリッジ リミテッド | 修飾ヌクレオシドまたは修飾ヌクレオチド |
| AU2017354876A1 (en) * | 2016-11-04 | 2019-06-20 | Qiagen Sciences, Llc | Thiol-containing cleave reagents and oxidative wash |
| BR112020010436A2 (pt) * | 2018-01-26 | 2020-11-24 | F. Hoffmann-La Roche Ag | oligonucleotídeo radiomarcado, processos para a preparação de um oligonucleotídeo radiomarcado, uso do oligonucleotídeo radiomarcado, método para determinar a biodistribuição e farmacocinética e oligonucleotídeo |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4910300A (en) * | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| US5783384A (en) * | 1992-01-13 | 1998-07-21 | President And Fellows Of Harvard College | Selection of binding-molecules |
| US5516664A (en) * | 1992-12-23 | 1996-05-14 | Hyman; Edward D. | Enzymatic synthesis of repeat regions of oligonucleotides |
| CA2241170A1 (en) * | 1995-12-27 | 1997-07-10 | Takara Shuzo Co., Ltd. | Novel dna polymerase |
| ATE269908T1 (de) * | 1997-04-01 | 2004-07-15 | Manteia S A | Methode zur sequenzierung von nukleinsäuren |
| US6485944B1 (en) * | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6280947B1 (en) * | 1999-08-11 | 2001-08-28 | Exact Sciences Corporation | Methods for detecting nucleotide insertion or deletion using primer extension |
| US6632655B1 (en) * | 1999-02-23 | 2003-10-14 | Caliper Technologies Corp. | Manipulation of microparticles in microfluidic systems |
| US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| AU2001265338A1 (en) * | 2000-06-02 | 2001-12-17 | Dna Sciences, Inc. | Primer extension using a mixture of labelled and unlabelled nucleotides |
-
2003
- 2003-04-04 EP EP03710595A patent/EP1495137B1/en not_active Expired - Lifetime
- 2003-04-04 US US10/510,107 patent/US20050244827A1/en not_active Abandoned
- 2003-04-04 DE DE60301622T patent/DE60301622T2/de not_active Expired - Lifetime
- 2003-04-04 AU AU2003214765A patent/AU2003214765B2/en not_active Expired
- 2003-04-04 JP JP2003582312A patent/JP4230364B2/ja not_active Expired - Fee Related
- 2003-04-04 CA CA2481495A patent/CA2481495C/en not_active Expired - Lifetime
- 2003-04-04 AT AT03710595T patent/ATE304610T1/de not_active IP Right Cessation
- 2003-04-04 WO PCT/SE2003/000547 patent/WO2003085135A1/en active IP Right Grant
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