DE60301622T2 - Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind - Google Patents

Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind Download PDF

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Publication number
DE60301622T2
DE60301622T2 DE60301622T DE60301622T DE60301622T2 DE 60301622 T2 DE60301622 T2 DE 60301622T2 DE 60301622 T DE60301622 T DE 60301622T DE 60301622 T DE60301622 T DE 60301622T DE 60301622 T2 DE60301622 T2 DE 60301622T2
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DE
Germany
Prior art keywords
nucleotide
primer
mole
linkers
labeled derivative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE60301622T
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English (en)
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DE60301622D1 (de
Inventor
Charlotta Olsson
Nigel Tooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
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Biotage AB
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Filing date
Publication date
Priority claimed from SE0201024A external-priority patent/SE0201024D0/xx
Application filed by Biotage AB filed Critical Biotage AB
Application granted granted Critical
Publication of DE60301622D1 publication Critical patent/DE60301622D1/de
Publication of DE60301622T2 publication Critical patent/DE60301622T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE60301622T 2002-04-04 2003-04-04 Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind Expired - Lifetime DE60301622T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36959902P 2002-04-04 2002-04-04
SE0201024A SE0201024D0 (sv) 2002-04-04 2002-04-04 New method
PCT/SE2003/000547 WO2003085135A1 (en) 2002-04-04 2003-04-04 New method

Publications (2)

Publication Number Publication Date
DE60301622D1 DE60301622D1 (de) 2005-10-20
DE60301622T2 true DE60301622T2 (de) 2006-06-14

Family

ID=28793693

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60301622T Expired - Lifetime DE60301622T2 (de) 2002-04-04 2003-04-04 Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind

Country Status (8)

Country Link
US (1) US20050244827A1 (de)
EP (1) EP1495137B1 (de)
JP (1) JP4230364B2 (de)
AT (1) ATE304610T1 (de)
AU (1) AU2003214765B2 (de)
CA (1) CA2481495C (de)
DE (1) DE60301622T2 (de)
WO (1) WO2003085135A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1337541B1 (de) 2000-10-06 2007-03-07 The Trustees of Columbia University in the City of New York Massives Parallelverfahren zur Dekodierung von DNA und RNA
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
ES2550513T3 (es) 2002-08-23 2015-11-10 Illumina Cambridge Limited Nucleótidos modificados para secuenciación de polinucleótidos
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
AU2004209001B2 (en) * 2003-01-29 2007-10-11 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
WO2005060345A2 (en) * 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
EP2071927A2 (de) 2006-09-28 2009-06-24 Illumina, Inc. Zusammensetzungen und verfahren zur nukleotidsequenzierung
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
US20110014611A1 (en) 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
EP2215156B1 (de) 2007-11-28 2013-01-09 Basf Se Flüssige stabilisatormischung
AU2009212165A1 (en) * 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. CIS reactive oxygen quenchers integrated into linkers
US20100255487A1 (en) 2009-03-27 2010-10-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
DK2970356T3 (en) 2013-03-15 2018-08-27 Illumina Cambridge Ltd Modified nucleosides or nucleotides
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
CA3041055A1 (en) * 2016-11-04 2018-05-11 Qiagen Sciences, Llc Thiol-containing cleave reagents and oxidative wash
CN111867638A (zh) * 2018-01-26 2020-10-30 豪夫迈·罗氏有限公司 放射性标记的寡核苷酸及其制备方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5783384A (en) * 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
KR100443780B1 (ko) * 1995-12-27 2004-09-18 다카라 바이오 가부시키가이샤 신규 dna 폴리머라제
EP1498494A3 (de) * 1997-04-01 2007-06-20 Solexa Ltd. Methode zur Sequenzierung von Nukleinsäuren
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
ATE556149T1 (de) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001094546A2 (en) * 2000-06-02 2001-12-13 Dna Sciences, Inc. Primer extension using a mixture of labelled and unlabelled nucleotides

Also Published As

Publication number Publication date
JP4230364B2 (ja) 2009-02-25
AU2003214765A1 (en) 2003-10-20
CA2481495A1 (en) 2003-10-16
US20050244827A1 (en) 2005-11-03
CA2481495C (en) 2011-08-30
AU2003214765B2 (en) 2007-07-26
EP1495137B1 (de) 2005-09-14
JP2005521422A (ja) 2005-07-21
EP1495137A1 (de) 2005-01-12
WO2003085135A1 (en) 2003-10-16
DE60301622D1 (de) 2005-10-20
ATE304610T1 (de) 2005-09-15

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Owner name: QIAGEN GMBH, 40724 HILDEN, DE