DE60301622D1 - Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind - Google Patents

Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind

Info

Publication number
DE60301622D1
DE60301622D1 DE60301622T DE60301622T DE60301622D1 DE 60301622 D1 DE60301622 D1 DE 60301622D1 DE 60301622 T DE60301622 T DE 60301622T DE 60301622 T DE60301622 T DE 60301622T DE 60301622 D1 DE60301622 D1 DE 60301622D1
Authority
DE
Germany
Prior art keywords
nucleotide
primer
mole
linkers
labeled derivative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60301622T
Other languages
English (en)
Other versions
DE60301622T2 (de
Inventor
Charlotta Olsson
Nigel Tooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Biotage AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0201024A external-priority patent/SE0201024D0/xx
Application filed by Biotage AB filed Critical Biotage AB
Publication of DE60301622D1 publication Critical patent/DE60301622D1/de
Application granted granted Critical
Publication of DE60301622T2 publication Critical patent/DE60301622T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE60301622T 2002-04-04 2003-04-04 Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind Expired - Lifetime DE60301622T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36959902P 2002-04-04 2002-04-04
SE0201024A SE0201024D0 (sv) 2002-04-04 2002-04-04 New method
PCT/SE2003/000547 WO2003085135A1 (en) 2002-04-04 2003-04-04 New method

Publications (2)

Publication Number Publication Date
DE60301622D1 true DE60301622D1 (de) 2005-10-20
DE60301622T2 DE60301622T2 (de) 2006-06-14

Family

ID=28793693

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60301622T Expired - Lifetime DE60301622T2 (de) 2002-04-04 2003-04-04 Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind

Country Status (8)

Country Link
US (1) US20050244827A1 (de)
EP (1) EP1495137B1 (de)
JP (1) JP4230364B2 (de)
AT (1) ATE304610T1 (de)
AU (1) AU2003214765B2 (de)
CA (1) CA2481495C (de)
DE (1) DE60301622T2 (de)
WO (1) WO2003085135A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002029003A2 (en) 2000-10-06 2002-04-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
SI3002289T1 (en) 2002-08-23 2018-07-31 Illumina Cambridge Limited MODIFIED NUCLEOTES FOR POLYUCULOTIDE SEQUENCING
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
WO2004069849A2 (en) * 2003-01-29 2004-08-19 454 Corporation Bead emulsion nucleic acid amplification
WO2005060345A2 (en) * 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
EP3431615A3 (de) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit nichtfluoreszierenden, reversiblen nukleotidterminatoren und spaltbaren, etikettmodifizierten nukleotidterminatoren
JP5431352B2 (ja) 2007-11-28 2014-03-05 ビーエーエスエフ ソシエタス・ヨーロピア 液体安定剤混合物
AU2009212165A1 (en) * 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. CIS reactive oxygen quenchers integrated into linkers
US8603792B2 (en) 2009-03-27 2013-12-10 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
JP6333297B2 (ja) 2013-03-15 2018-05-30 イルミナ ケンブリッジ リミテッド 修飾ヌクレオシドまたは修飾ヌクレオチド
AU2017354876A1 (en) * 2016-11-04 2019-06-20 Qiagen Sciences, Llc Thiol-containing cleave reagents and oxidative wash
BR112020010436A2 (pt) * 2018-01-26 2020-11-24 F. Hoffmann-La Roche Ag oligonucleotídeo radiomarcado, processos para a preparação de um oligonucleotídeo radiomarcado, uso do oligonucleotídeo radiomarcado, método para determinar a biodistribuição e farmacocinética e oligonucleotídeo

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5783384A (en) * 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
CA2241170A1 (en) * 1995-12-27 1997-07-10 Takara Shuzo Co., Ltd. Novel dna polymerase
ATE269908T1 (de) * 1997-04-01 2004-07-15 Manteia S A Methode zur sequenzierung von nukleinsäuren
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6632655B1 (en) * 1999-02-23 2003-10-14 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
AU2001265338A1 (en) * 2000-06-02 2001-12-17 Dna Sciences, Inc. Primer extension using a mixture of labelled and unlabelled nucleotides

Also Published As

Publication number Publication date
JP4230364B2 (ja) 2009-02-25
WO2003085135A1 (en) 2003-10-16
AU2003214765A1 (en) 2003-10-20
ATE304610T1 (de) 2005-09-15
JP2005521422A (ja) 2005-07-21
US20050244827A1 (en) 2005-11-03
DE60301622T2 (de) 2006-06-14
AU2003214765B2 (en) 2007-07-26
EP1495137A1 (de) 2005-01-12
CA2481495C (en) 2011-08-30
EP1495137B1 (de) 2005-09-14
CA2481495A1 (en) 2003-10-16

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: QIAGEN GMBH, 40724 HILDEN, DE