JP2004535189A - 組換えタンパク質の高レベルの大規模生成のための組成物および方法 - Google Patents

組換えタンパク質の高レベルの大規模生成のための組成物および方法 Download PDF

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JP2004535189A
JP2004535189A JP2003502199A JP2003502199A JP2004535189A JP 2004535189 A JP2004535189 A JP 2004535189A JP 2003502199 A JP2003502199 A JP 2003502199A JP 2003502199 A JP2003502199 A JP 2003502199A JP 2004535189 A JP2004535189 A JP 2004535189A
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polypeptide
vector
host cell
ucoe
composition
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JP2004535189A5 (cg-RX-API-DMAC7.html
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トリッシュ ベントン，
クリストファー ロバート ベビントン，
カーラ アン ヘニング，
デービッド ジェイ． キング，
ロバート クロンビー，
シャオ シャン，
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エムエル ラボラトリーズ ピーエルシー
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2800/00—Nucleic acids vectors
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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    • C12N2840/00—Vectors comprising a special translation-regulating system
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2003502199A 2001-06-04 2002-06-04 組換えタンパク質の高レベルの大規模生成のための組成物および方法 Pending JP2004535189A (ja)

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US29596101P 2001-06-04 2001-06-04
US33362001P 2001-11-26 2001-11-26
US35240402P 2002-01-29 2002-01-29
PCT/US2002/017763 WO2002099089A1 (en) 2001-06-04 2002-06-04 Compositions and methods for high-level, large-scale production of recombinant proteins

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JP2004535189A true JP2004535189A (ja) 2004-11-25
JP2004535189A5 JP2004535189A5 (cg-RX-API-DMAC7.html) 2006-01-05

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US (1) US20040161817A1 (cg-RX-API-DMAC7.html)
EP (1) EP1402006A4 (cg-RX-API-DMAC7.html)
JP (1) JP2004535189A (cg-RX-API-DMAC7.html)
KR (1) KR20040032105A (cg-RX-API-DMAC7.html)
CN (1) CN1533432A (cg-RX-API-DMAC7.html)
AU (1) AU2002310321A1 (cg-RX-API-DMAC7.html)
CA (1) CA2463310A1 (cg-RX-API-DMAC7.html)
WO (2) WO2002099089A1 (cg-RX-API-DMAC7.html)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008539781A (ja) * 2005-05-17 2008-11-20 ミリポア・コーポレイション 改良された発現エレメント
JP2008544750A (ja) * 2005-06-30 2008-12-11 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2012515922A (ja) * 2009-01-22 2012-07-12 モメンタ ファーマシューティカルズ インコーポレイテッド ＣＨＯ細胞由来の糖タンパク質産物におけるガラクトース−α−１，３−ガラクトース含有Ｎ−グリカン
WO2019225372A1 (ja) * 2018-05-24 2019-11-28 国立大学法人北海道大学 新規ベクターおよびその利用

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US7812148B2 (en) 2001-04-05 2010-10-12 Millipore Corporation Vectors comprising CpG islands without position effect varigation and having increased expression
WO2004056986A2 (en) 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
ES2345335T3 (es) 2003-02-01 2010-09-21 Millipore Corporation Elementos geneticos mejorados que proporcionan altos niveles de expresion.
US7824907B2 (en) * 2003-03-11 2010-11-02 Merck Serono Sa Expression vectors comprising the mCMV IE2 promoter
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
JP5291341B2 (ja) 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定
JP4642865B2 (ja) * 2005-03-05 2011-03-02 ミリポア・コーポレイション 新規制御エレメントを含むベクター
GB0504587D0 (en) * 2005-03-05 2005-04-13 Ml Lab Plc Vectors comprising guinea pig CMV regulatory elements
EP1996705B1 (en) 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
JP2016500255A (ja) 2012-12-11 2016-01-12 アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ ハイスループットなレセプター：リガンド同定の方法
WO2014134412A1 (en) 2013-03-01 2014-09-04 Regents Of The University Of Minnesota Talen-based gene correction
CN104341505A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗egfr的人鼠嵌合抗体
CN104341503A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗cd20的人抗体
KR102488477B1 (ko) * 2014-01-21 2023-01-13 앨버트 아인슈타인 컬리지 오브 메디신 신속하고 포괄적인 t-세포 면역 모니터링을 위한 세포 플랫폼
US11505591B2 (en) 2016-05-18 2022-11-22 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
KR20190044029A (ko) 2016-05-18 2019-04-29 알버트 아인슈타인 컬리지 오브 메디슨, 인크. 변이체 pd-l1 폴리펩타이드, t-세포 조절 다량체 폴리펩타이드 및 이들의 사용 방법
DK3558339T5 (da) 2016-12-22 2024-09-02 Cue Biopharma Inc T-celle-modulerende multimere polypeptider og fremgangsmåder til anvendelse deraf
EP3565829A4 (en) 2017-01-09 2021-01-27 Cue Biopharma, Inc. T-CELL-MODULATING MULTIMERIC POLYPEPTIDES AND METHOD OF USING THEREOF
US20200010528A1 (en) 2017-03-15 2020-01-09 Cue Biopharma, Inc. Methods for modulating an immune response
CN111886241A (zh) 2018-01-09 2020-11-03 库尔生物制药有限公司 多聚体t细胞调节多肽及其使用方法
IL296209A (en) 2020-05-12 2022-11-01 Cue Biopharma Inc Multimeric t-cell modulatory polypeptides and methods of using them
EP4211149A4 (en) 2020-09-09 2024-10-09 Cue Biopharma, Inc. MULTIMERIC POLYPEPTIDES MODULATING MHC CLASS II T CELLS FOR THE TREATMENT OF TYPE 1 DIABETES MELLITUS (T1D) AND METHODS OF USE THEREOF

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IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
SE9303601D0 (sv) * 1993-11-01 1993-11-01 Kabi Pharmacia Ab Improved cell cultivation method and medium
CN100365127C (zh) * 1998-07-21 2008-01-30 密理博公司 具有遍在染色质开放表达元件(ucoe)的聚核苷酸

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008539781A (ja) * 2005-05-17 2008-11-20 ミリポア・コーポレイション 改良された発現エレメント
JP2008544750A (ja) * 2005-06-30 2008-12-11 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2016154537A (ja) * 2005-06-30 2016-09-01 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2019058173A (ja) * 2005-06-30 2019-04-18 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2012515922A (ja) * 2009-01-22 2012-07-12 モメンタ ファーマシューティカルズ インコーポレイテッド ＣＨＯ細胞由来の糖タンパク質産物におけるガラクトース−α−１，３−ガラクトース含有Ｎ−グリカン
WO2019225372A1 (ja) * 2018-05-24 2019-11-28 国立大学法人北海道大学 新規ベクターおよびその利用
JPWO2019225372A1 (ja) * 2018-05-24 2021-06-10 国立大学法人北海道大学 新規ベクターおよびその利用
JP7264353B2 (ja) 2018-05-24 2023-04-25 国立大学法人北海道大学 新規ベクターおよびその利用
US11781146B2 (en) 2018-05-24 2023-10-10 National University Corporation Hokkaido University Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element
AU2019273200B2 (en) * 2018-05-24 2024-10-31 Fuso Pharmaceutical Industries,Ltd. Novel vector and use thereof

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AU2002310321A8 (en) 2008-01-10
EP1402006A1 (en) 2004-03-31
EP1402006A4 (en) 2005-11-23
KR20040032105A (ko) 2004-04-14
CA2463310A1 (en) 2002-12-12
WO2002099070A2 (en) 2002-12-12
WO2002099089A1 (en) 2002-12-12
AU2002310321A1 (en) 2002-12-16
CN1533432A (zh) 2004-09-29
US20040161817A1 (en) 2004-08-19
WO2002099070A3 (en) 2007-11-15

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