CA2463310A1 - Compositions and methods for high-level, large-scale production of recombinant proteins - Google Patents
Compositions and methods for high-level, large-scale production of recombinant proteins Download PDFInfo
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- CA2463310A1 CA2463310A1 CA002463310A CA2463310A CA2463310A1 CA 2463310 A1 CA2463310 A1 CA 2463310A1 CA 002463310 A CA002463310 A CA 002463310A CA 2463310 A CA2463310 A CA 2463310A CA 2463310 A1 CA2463310 A1 CA 2463310A1
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Classifications
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
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- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
- C12N2830/205—Vector systems having a special element relevant for transcription transcription of more than one cistron bidirectional
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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- Life Sciences & Earth Sciences (AREA)
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- Wood Science & Technology (AREA)
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- Microbiology (AREA)
- Biochemistry (AREA)
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- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
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| US29596101P | 2001-06-04 | 2001-06-04 | |
| US60/295,961 | 2001-06-04 | ||
| US33362001P | 2001-11-26 | 2001-11-26 | |
| US60/333,620 | 2001-11-26 | ||
| US35240402P | 2002-01-29 | 2002-01-29 | |
| US60/352,404 | 2002-01-29 | ||
| PCT/US2002/017763 WO2002099089A1 (en) | 2001-06-04 | 2002-06-04 | Compositions and methods for high-level, large-scale production of recombinant proteins |
Publications (1)
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|---|---|
| CA2463310A1 true CA2463310A1 (en) | 2002-12-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CA002463310A Abandoned CA2463310A1 (en) | 2001-06-04 | 2002-06-04 | Compositions and methods for high-level, large-scale production of recombinant proteins |
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| US (1) | US20040161817A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1402006A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2004535189A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20040032105A (cg-RX-API-DMAC7.html) |
| CN (1) | CN1533432A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2002310321A1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2463310A1 (cg-RX-API-DMAC7.html) |
| WO (2) | WO2002099089A1 (cg-RX-API-DMAC7.html) |
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| US7812148B2 (en) | 2001-04-05 | 2010-10-12 | Millipore Corporation | Vectors comprising CpG islands without position effect varigation and having increased expression |
| WO2004056986A2 (en) | 2002-12-20 | 2004-07-08 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
| ES2345335T3 (es) | 2003-02-01 | 2010-09-21 | Millipore Corporation | Elementos geneticos mejorados que proporcionan altos niveles de expresion. |
| US7824907B2 (en) * | 2003-03-11 | 2010-11-02 | Merck Serono Sa | Expression vectors comprising the mCMV IE2 promoter |
| US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| SI1809750T1 (sl) | 2004-11-08 | 2012-08-31 | Chromagenics Bv | Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina |
| US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| JP5291341B2 (ja) | 2004-11-08 | 2013-09-18 | クロマジェニックス ベー ヴェー | タンパク質を高レベルで発現する宿主細胞の選定 |
| JP4642865B2 (ja) * | 2005-03-05 | 2011-03-02 | ミリポア・コーポレイション | 新規制御エレメントを含むベクター |
| GB0504587D0 (en) * | 2005-03-05 | 2005-04-13 | Ml Lab Plc | Vectors comprising guinea pig CMV regulatory elements |
| GB0509965D0 (en) * | 2005-05-17 | 2005-06-22 | Ml Lab Plc | Improved expression elements |
| EP1739179A1 (en) * | 2005-06-30 | 2007-01-03 | Octapharma AG | Serum-free stable transfection and production of recombinant human proteins in human cell lines |
| EP1996705B1 (en) | 2006-03-20 | 2011-08-31 | ChromaGenics B.V. | Expression augmenting dna fragments, use thereof, and methods for finding thereof |
| WO2010085251A1 (en) | 2009-01-22 | 2010-07-29 | Momenta Pharmaceuticals, Inc. | Galactose-alpha-1, 3-galactose-containing n-glycans in glycoprotein products derived from cho cells |
| JP2016500255A (ja) | 2012-12-11 | 2016-01-12 | アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ | ハイスループットなレセプター：リガンド同定の方法 |
| WO2014134412A1 (en) | 2013-03-01 | 2014-09-04 | Regents Of The University Of Minnesota | Talen-based gene correction |
| CN104341505A (zh) * | 2013-07-29 | 2015-02-11 | 西藏海思科药业集团股份有限公司 | 对蒙古人种和高加索人种低免疫原性的、抗egfr的人鼠嵌合抗体 |
| CN104341503A (zh) * | 2013-07-29 | 2015-02-11 | 西藏海思科药业集团股份有限公司 | 对蒙古人种和高加索人种低免疫原性的、抗cd20的人抗体 |
| KR102488477B1 (ko) * | 2014-01-21 | 2023-01-13 | 앨버트 아인슈타인 컬리지 오브 메디신 | 신속하고 포괄적인 t-세포 면역 모니터링을 위한 세포 플랫폼 |
| US11505591B2 (en) | 2016-05-18 | 2022-11-22 | Cue Biopharma, Inc. | T-cell modulatory multimeric polypeptides and methods of use thereof |
| KR20190044029A (ko) | 2016-05-18 | 2019-04-29 | 알버트 아인슈타인 컬리지 오브 메디슨, 인크. | 변이체 pd-l1 폴리펩타이드, t-세포 조절 다량체 폴리펩타이드 및 이들의 사용 방법 |
| DK3558339T5 (da) | 2016-12-22 | 2024-09-02 | Cue Biopharma Inc | T-celle-modulerende multimere polypeptider og fremgangsmåder til anvendelse deraf |
| EP3565829A4 (en) | 2017-01-09 | 2021-01-27 | Cue Biopharma, Inc. | T-CELL-MODULATING MULTIMERIC POLYPEPTIDES AND METHOD OF USING THEREOF |
| US20200010528A1 (en) | 2017-03-15 | 2020-01-09 | Cue Biopharma, Inc. | Methods for modulating an immune response |
| CN111886241A (zh) | 2018-01-09 | 2020-11-03 | 库尔生物制药有限公司 | 多聚体t细胞调节多肽及其使用方法 |
| CA3099955A1 (en) | 2018-05-24 | 2019-11-28 | National University Corporation Hokkaido University | Novel vector and use thereof |
| IL296209A (en) | 2020-05-12 | 2022-11-01 | Cue Biopharma Inc | Multimeric t-cell modulatory polypeptides and methods of using them |
| EP4211149A4 (en) | 2020-09-09 | 2024-10-09 | Cue Biopharma, Inc. | MULTIMERIC POLYPEPTIDES MODULATING MHC CLASS II T CELLS FOR THE TREATMENT OF TYPE 1 DIABETES MELLITUS (T1D) AND METHODS OF USE THEREOF |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO162160C (no) * | 1987-01-09 | 1989-11-15 | Medi Cult As | Serumfritt vekstmedium, samt anvendelse derav. |
| IL87737A (en) * | 1987-09-11 | 1993-08-18 | Genentech Inc | Method for culturing polypeptide factor dependent vertebrate recombinant cells |
| SE9303601D0 (sv) * | 1993-11-01 | 1993-11-01 | Kabi Pharmacia Ab | Improved cell cultivation method and medium |
| CN100365127C (zh) * | 1998-07-21 | 2008-01-30 | 密理博公司 | 具有遍在染色质开放表达元件(ucoe)的聚核苷酸 |
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- 2002-06-04 JP JP2003502199A patent/JP2004535189A/ja active Pending
- 2002-06-04 CA CA002463310A patent/CA2463310A1/en not_active Abandoned
- 2002-06-04 EP EP02734688A patent/EP1402006A4/en not_active Withdrawn
- 2002-06-04 WO PCT/US2002/017763 patent/WO2002099089A1/en not_active Ceased
- 2002-06-04 WO PCT/US2002/017770 patent/WO2002099070A2/en not_active Ceased
- 2002-06-04 US US10/163,863 patent/US20040161817A1/en not_active Abandoned
- 2002-06-04 AU AU2002310321A patent/AU2002310321A1/en not_active Abandoned
- 2002-06-04 CN CNA028143825A patent/CN1533432A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002310321A8 (en) | 2008-01-10 |
| EP1402006A1 (en) | 2004-03-31 |
| EP1402006A4 (en) | 2005-11-23 |
| KR20040032105A (ko) | 2004-04-14 |
| WO2002099070A2 (en) | 2002-12-12 |
| WO2002099089A1 (en) | 2002-12-12 |
| JP2004535189A (ja) | 2004-11-25 |
| AU2002310321A1 (en) | 2002-12-16 |
| CN1533432A (zh) | 2004-09-29 |
| US20040161817A1 (en) | 2004-08-19 |
| WO2002099070A3 (en) | 2007-11-15 |
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