CN1533432A - 高水平、大规模生产重组蛋白的组合体和方法 - Google Patents

高水平、大规模生产重组蛋白的组合体和方法 Download PDF

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Publication number
CN1533432A
CN1533432A CNA028143825A CN02814382A CN1533432A CN 1533432 A CN1533432 A CN 1533432A CN A028143825 A CNA028143825 A CN A028143825A CN 02814382 A CN02814382 A CN 02814382A CN 1533432 A CN1533432 A CN 1533432A
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China
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polypeptide
cell line
host cell
expression
vector
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Pending
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Chinese (zh)
Inventor
��ʲ
特里什·本顿
���ء����߸�
克里斯多佛·罗伯特·贝宾顿
卡尔拉·安·亨宁
J
戴维·J·金
罗伯特·克龙比
向少
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Innovata Ltd
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ML Laboratories PLC
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2800/00—Nucleic acids vectors
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    • C12N2800/108—Plasmid DNA episomal vectors
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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    • C12N2840/00—Vectors comprising a special translation-regulating system
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNA028143825A 2001-06-04 2002-06-04 高水平、大规模生产重组蛋白的组合体和方法 Pending CN1533432A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US29596101P 2001-06-04 2001-06-04
US60/295,961 2001-06-04
US33362001P 2001-11-26 2001-11-26
US60/333,620 2001-11-26
US35240402P 2002-01-29 2002-01-29
US60/352,404 2002-01-29

Publications (1)

Publication Number Publication Date
CN1533432A true CN1533432A (zh) 2004-09-29

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ID=27404392

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CNA028143825A Pending CN1533432A (zh) 2001-06-04 2002-06-04 高水平、大规模生产重组蛋白的组合体和方法

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US (1) US20040161817A1 (cg-RX-API-DMAC7.html)
EP (1) EP1402006A4 (cg-RX-API-DMAC7.html)
JP (1) JP2004535189A (cg-RX-API-DMAC7.html)
KR (1) KR20040032105A (cg-RX-API-DMAC7.html)
CN (1) CN1533432A (cg-RX-API-DMAC7.html)
AU (1) AU2002310321A1 (cg-RX-API-DMAC7.html)
CA (1) CA2463310A1 (cg-RX-API-DMAC7.html)
WO (2) WO2002099089A1 (cg-RX-API-DMAC7.html)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104341505A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗egfr的人鼠嵌合抗体
CN104341503A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗cd20的人抗体

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US7812148B2 (en) 2001-04-05 2010-10-12 Millipore Corporation Vectors comprising CpG islands without position effect varigation and having increased expression
WO2004056986A2 (en) 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
ES2345335T3 (es) 2003-02-01 2010-09-21 Millipore Corporation Elementos geneticos mejorados que proporcionan altos niveles de expresion.
US7824907B2 (en) * 2003-03-11 2010-11-02 Merck Serono Sa Expression vectors comprising the mCMV IE2 promoter
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
JP5291341B2 (ja) 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定
JP4642865B2 (ja) * 2005-03-05 2011-03-02 ミリポア・コーポレイション 新規制御エレメントを含むベクター
GB0504587D0 (en) * 2005-03-05 2005-04-13 Ml Lab Plc Vectors comprising guinea pig CMV regulatory elements
GB0509965D0 (en) * 2005-05-17 2005-06-22 Ml Lab Plc Improved expression elements
EP1739179A1 (en) * 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
EP1996705B1 (en) 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
WO2010085251A1 (en) 2009-01-22 2010-07-29 Momenta Pharmaceuticals, Inc. Galactose-alpha-1, 3-galactose-containing n-glycans in glycoprotein products derived from cho cells
JP2016500255A (ja) 2012-12-11 2016-01-12 アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ ハイスループットなレセプター：リガンド同定の方法
WO2014134412A1 (en) 2013-03-01 2014-09-04 Regents Of The University Of Minnesota Talen-based gene correction
KR102488477B1 (ko) * 2014-01-21 2023-01-13 앨버트 아인슈타인 컬리지 오브 메디신 신속하고 포괄적인 t-세포 면역 모니터링을 위한 세포 플랫폼
US11505591B2 (en) 2016-05-18 2022-11-22 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
KR20190044029A (ko) 2016-05-18 2019-04-29 알버트 아인슈타인 컬리지 오브 메디슨, 인크. 변이체 pd-l1 폴리펩타이드, t-세포 조절 다량체 폴리펩타이드 및 이들의 사용 방법
DK3558339T5 (da) 2016-12-22 2024-09-02 Cue Biopharma Inc T-celle-modulerende multimere polypeptider og fremgangsmåder til anvendelse deraf
EP3565829A4 (en) 2017-01-09 2021-01-27 Cue Biopharma, Inc. T-CELL-MODULATING MULTIMERIC POLYPEPTIDES AND METHOD OF USING THEREOF
US20200010528A1 (en) 2017-03-15 2020-01-09 Cue Biopharma, Inc. Methods for modulating an immune response
CN111886241A (zh) 2018-01-09 2020-11-03 库尔生物制药有限公司 多聚体t细胞调节多肽及其使用方法
CA3099955A1 (en) 2018-05-24 2019-11-28 National University Corporation Hokkaido University Novel vector and use thereof
IL296209A (en) 2020-05-12 2022-11-01 Cue Biopharma Inc Multimeric t-cell modulatory polypeptides and methods of using them
EP4211149A4 (en) 2020-09-09 2024-10-09 Cue Biopharma, Inc. MULTIMERIC POLYPEPTIDES MODULATING MHC CLASS II T CELLS FOR THE TREATMENT OF TYPE 1 DIABETES MELLITUS (T1D) AND METHODS OF USE THEREOF

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NO162160C (no) * 1987-01-09 1989-11-15 Medi Cult As Serumfritt vekstmedium, samt anvendelse derav.
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
SE9303601D0 (sv) * 1993-11-01 1993-11-01 Kabi Pharmacia Ab Improved cell cultivation method and medium
CN100365127C (zh) * 1998-07-21 2008-01-30 密理博公司 具有遍在染色质开放表达元件(ucoe)的聚核苷酸

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104341505A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗egfr的人鼠嵌合抗体
CN104341503A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗cd20的人抗体

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AU2002310321A8 (en) 2008-01-10
EP1402006A1 (en) 2004-03-31
EP1402006A4 (en) 2005-11-23
KR20040032105A (ko) 2004-04-14
CA2463310A1 (en) 2002-12-12
WO2002099070A2 (en) 2002-12-12
WO2002099089A1 (en) 2002-12-12
JP2004535189A (ja) 2004-11-25
AU2002310321A1 (en) 2002-12-16
US20040161817A1 (en) 2004-08-19
WO2002099070A3 (en) 2007-11-15

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