JP2004532642A5 - - Google Patents
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- Publication number
- JP2004532642A5 JP2004532642A5 JP2003503770A JP2003503770A JP2004532642A5 JP 2004532642 A5 JP2004532642 A5 JP 2004532642A5 JP 2003503770 A JP2003503770 A JP 2003503770A JP 2003503770 A JP2003503770 A JP 2003503770A JP 2004532642 A5 JP2004532642 A5 JP 2004532642A5
- Authority
- JP
- Japan
- Prior art keywords
- culture
- medium
- during
- glucose
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 35
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 16
- 239000008103 glucose Substances 0.000 claims 16
- 239000002609 medium Substances 0.000 claims 15
- 239000006143 cell culture medium Substances 0.000 claims 12
- 239000000654 additive Substances 0.000 claims 5
- 230000000996 additive effect Effects 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 5
- 238000012258 culturing Methods 0.000 claims 5
- 239000002054 inoculum Substances 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 3
- 210000004102 animal cell Anatomy 0.000 claims 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 3
- 238000010899 nucleation Methods 0.000 claims 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 235000013922 glutamic acid Nutrition 0.000 claims 2
- 239000004220 glutamic acid Substances 0.000 claims 2
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 claims 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims 1
- 101150013553 CD40 gene Proteins 0.000 claims 1
- 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 claims 1
- 235000021120 animal protein Nutrition 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 229930195712 glutamate Natural products 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29801301P | 2001-06-13 | 2001-06-13 | |
| PCT/US2002/018687 WO2002101019A2 (en) | 2001-06-13 | 2002-06-11 | Methods of culturing animal cells and polypeptide production in animal cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004532642A JP2004532642A (ja) | 2004-10-28 |
| JP2004532642A5 true JP2004532642A5 (enExample) | 2006-01-05 |
Family
ID=23148629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003503770A Pending JP2004532642A (ja) | 2001-06-13 | 2002-06-11 | 動物細胞の培養方法と動物細胞でのポリペプチド産生 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20030087372A1 (enExample) |
| EP (1) | EP1404813A4 (enExample) |
| JP (1) | JP2004532642A (enExample) |
| AU (1) | AU2002316230A1 (enExample) |
| CA (1) | CA2447791A1 (enExample) |
| WO (1) | WO2002101019A2 (enExample) |
Families Citing this family (70)
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| US20060235209A9 (en) * | 1997-03-10 | 2006-10-19 | Jin-An Jiao | Use of anti-tissue factor antibodies for treating thromboses |
| US20030109680A1 (en) * | 2001-11-21 | 2003-06-12 | Sunol Molecular Corporation | Antibodies for inhibiting blood coagulation and methods of use thereof |
| US5986065A (en) * | 1997-03-10 | 1999-11-16 | Sunol Molecular Corporation | Antibodies for inhibiting blood coagulation and methods of use thereof |
| US7749498B2 (en) * | 1997-03-10 | 2010-07-06 | Genentech, Inc. | Antibodies for inhibiting blood coagulation and methods of use thereof |
| US6900056B2 (en) | 2001-02-15 | 2005-05-31 | Centocor, Inc. | Chemically defined medium for cultured mammalian cells |
| CA2520891C (en) * | 2003-05-09 | 2014-07-08 | Crucell Holland B.V. | Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom |
| EP1623019B2 (en) * | 2003-05-15 | 2017-01-25 | Wyeth LLC | Restricted glucose feed for animal cell culture |
| WO2005004793A2 (en) * | 2003-06-19 | 2005-01-20 | Tanox Inc. | Compositions and methods for treating coagulation related disorders |
| EP1673452B1 (en) * | 2003-10-10 | 2015-12-23 | Novo Nordisk Health Care AG | Method for large-scale production of a polypeptide in eukaryote cells |
| US7300773B2 (en) * | 2004-08-27 | 2007-11-27 | Wyeth Research Ireland Limited | Production of TNFR-Ig |
| RU2451082C2 (ru) * | 2004-08-27 | 2012-05-20 | Вайет Рисёрч Айрлэнд Лимитед | Производство полипептидов |
| TWI384069B (zh) * | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| AU2012200353B2 (en) * | 2004-08-27 | 2014-06-19 | Pfizer Ireland Pharmaceuticals | PRODUCTION OF a-ABeta |
| US7335491B2 (en) * | 2004-08-27 | 2008-02-26 | Wyeth Research Ireland Limited | Production of anti-abeta |
| PL3121266T3 (pl) | 2006-01-04 | 2020-07-13 | Baxalta Incorporated | Wolne od oligopeptydów pożywki do hodowli komórkowej |
| US20070190057A1 (en) | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
| AR059065A1 (es) * | 2006-01-23 | 2008-03-12 | Amgen Inc | Metodos para modular el contenido de manosa de las proteinas recombinantes |
| CN103276033A (zh) | 2006-09-13 | 2013-09-04 | 雅培制药有限公司 | 细胞培养改良 |
| HUE026403T2 (en) | 2007-03-02 | 2016-06-28 | Wyeth Llc | Use of copper and glutamate in cell culture to produce polypeptides |
| WO2008121615A2 (en) * | 2007-03-30 | 2008-10-09 | Medimmune, Inc. | Antibody formulation |
| TWI601823B (zh) | 2007-04-26 | 2017-10-11 | Chugai Pharmaceutical Co Ltd | Cell culture methods using media containing high concentrations of amino acids |
| HUE046992T2 (hu) | 2007-07-09 | 2020-04-28 | Genentech Inc | Diszulfidkötés redukciójának megelõzése polipeptidek rekombináns elõállítása során |
| US20090042253A1 (en) | 2007-08-09 | 2009-02-12 | Wyeth | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| SI2310523T1 (sl) * | 2008-04-17 | 2015-10-30 | Wyeth Llc | Postopki za boljšo proizvodnjo kostnih morfogenetskih proteinov |
| WO2010016943A2 (en) | 2008-08-08 | 2010-02-11 | Biogen Idec Ma Inc. | Nutrient monitoring and feedback control for increased bioproduct production |
| SI2464725T2 (sl) | 2009-08-11 | 2025-06-30 | F. Hoffmann-La Roche Ag | Proizvodnja beljakovin v medijih za celične kulture brez glutamina |
| ES2622366T3 (es) | 2009-10-26 | 2017-07-06 | F. Hoffmann-La Roche Ag | Procedimiento para la producción de una inmunoglobulina glucosilada |
| WO2011062926A2 (en) | 2009-11-17 | 2011-05-26 | Medarex, Inc. | Methods for enhanced protein production |
| WO2011085095A2 (en) * | 2010-01-07 | 2011-07-14 | Dr. Reddy's Laboratories Ltd. | Enhanced protein expression |
| RU2644651C2 (ru) | 2010-04-26 | 2018-02-13 | Новартис Аг | Среда для культивирования клеток |
| ES2659117T3 (es) | 2010-04-26 | 2018-03-13 | Novartis Ag | Proceso de cultivo celular de CHO |
| MX2012012525A (es) | 2010-04-26 | 2012-11-23 | Novartis Ag | Medio de cultivo mejorado. |
| CN103282042B (zh) * | 2010-09-17 | 2014-12-10 | 巴克斯特国际公司 | 通过具有组氨酸的水性制剂在弱酸性至中性pH稳定的免疫球蛋白 |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| KR101591671B1 (ko) * | 2011-04-29 | 2016-02-04 | 바이오콘 리서치 리미티드 | 항체의 이질성을 감소시키는 방법 및 그 항체의 제조방법 |
| WO2012147048A2 (en) * | 2011-04-29 | 2012-11-01 | Biocon Research Limited | Methods for reducing accumulation of lactate during culturing and method for producing polypeptide |
| IL288509B2 (en) | 2011-07-01 | 2023-04-01 | Amgen Inc | Mammalian cell culture |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US9150645B2 (en) | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| RU2496869C1 (ru) * | 2012-04-23 | 2013-10-27 | Государственное научное учреждение Прикаспийский зональный научно-исследовательский ветеринарный институт Российской академии сельскохозяйственных наук | Способ культивирования клеток человека и животных |
| US20130281355A1 (en) | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| EP2830651A4 (en) | 2013-03-12 | 2015-09-02 | Abbvie Inc | HUMAN ANTIBODIES THAT BIND TNF-ALPHA AND PREPARATION METHODS |
| US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| KR20210037745A (ko) | 2013-03-15 | 2021-04-06 | 제넨테크, 인크. | 항산화제를 함유하는 세포 배양 조성물 및 폴리펩티드 생산 방법 |
| CA2913687C (en) | 2013-07-04 | 2022-12-13 | F. Hoffmann-La Roche Ag | Interference-suppressed immunoassay to detect anti-drug antibodies in serum samples |
| EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| KR102255902B1 (ko) * | 2014-01-29 | 2021-05-26 | 주식회사 엘지화학 | 배양배지의 최적화를 통한 재조합 단백질의 갈락토오스화를 조절하는 방법 |
| EP3110941A4 (en) * | 2014-02-25 | 2017-10-18 | Dr. Reddy's Laboratories Ltd. | Process for modifying galactosylation and g0f content of a glycoprotein composition by glutamine supplementation |
| MA40864A (fr) | 2014-10-31 | 2017-09-05 | Biogen Ma Inc | Hypotaurine, gaba, bêta-alanine et choline pour la régulation de l'accumulation de sous-produits résiduaires dans des procédés de culture de cellules mammifères |
| WO2016106192A2 (en) | 2014-12-22 | 2016-06-30 | Genzyme Corporation | Methods of culturing a mammalian cell |
| TW202440903A (zh) | 2015-08-04 | 2024-10-16 | 美商再生元醫藥公司 | 補充牛磺酸之細胞培養基及用法（一） |
| CN111954719B (zh) | 2018-03-26 | 2025-07-18 | 美国安进公司 | 细胞培养物中产生的抗体的总去岩藻糖基化糖型 |
| JP2020005511A (ja) * | 2018-07-03 | 2020-01-16 | 株式会社日立製作所 | 培養方法及び培養装置 |
| EP3969564A1 (en) * | 2019-05-16 | 2022-03-23 | Formycon AG | Method for reducing methionine oxidation in recombinant proteins |
| CN110257319A (zh) * | 2019-07-26 | 2019-09-20 | 苏州瑞徕生物科技有限公司 | 一种微载体上细胞维持培养的培养液及方法 |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| US11186625B2 (en) | 2019-12-06 | 2021-11-30 | Regeneran Pharmaceuticals, Inc. | Anti-VEGF protein compositions and methods for producing the same |
| AU2021268026A1 (en) | 2020-05-08 | 2023-01-19 | Regeneron Pharmaceuticals, Inc. | VEGF traps and mini-traps and methods for treating ocular disorders and cancer |
| US20230323422A1 (en) * | 2020-08-14 | 2023-10-12 | Bristol-Myers Squibb Company | Method of making protein |
| EP4323500A1 (en) * | 2021-04-14 | 2024-02-21 | Genzyme Corporation | Methods of perfusion culturing a mammalian cell |
| TW202309081A (zh) * | 2021-04-27 | 2023-03-01 | 美商安進公司 | 藉由使用溫度調節不對稱多特異性抗體之產物品質 |
| CN115927160B (zh) * | 2022-12-28 | 2025-05-30 | 无锡药明生物技术股份有限公司 | 细胞培养基测试方法及其在生物制药中的应用 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US30985A (en) * | 1860-12-18 | Thomas l | ||
| US3926723A (en) * | 1974-08-08 | 1975-12-16 | Massachusetts Inst Technology | Method of controllably releasing glucose to a cell culture medium |
| US4560655A (en) * | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) * | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4767704A (en) * | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US4724206A (en) * | 1984-02-13 | 1988-02-09 | Damon Biotech, Inc. | Protein production using hypertonic media |
| US5672502A (en) * | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| US4927762A (en) * | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| US6238891B1 (en) * | 1987-11-18 | 2001-05-29 | Cetus Oncology Corporation | Method of increasing product expression through solute stress |
| US5151359A (en) * | 1988-05-19 | 1992-09-29 | Mitsui Toatsu Chemicals Incorporated | Method for producing of human tissue type plasminogen activator |
| US5122469A (en) * | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
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| US5736137A (en) * | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| US5856179A (en) * | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| US5721121A (en) * | 1995-06-06 | 1998-02-24 | Genentech, Inc. | Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein |
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| JP4306813B2 (ja) * | 1995-09-19 | 2009-08-05 | アスビオファーマ株式会社 | 動物細胞の新規培養方法 |
| US6087129A (en) * | 1996-01-19 | 2000-07-11 | Betagene, Inc. | Recombinant expression of proteins from secretory cell lines |
| WO1998041611A1 (en) * | 1997-03-20 | 1998-09-24 | Regents Of The University Of Minnesota | Process for the continuous culture of cells |
| US6237791B1 (en) * | 1997-04-09 | 2001-05-29 | Dtl Technology Limited Partnership | Wide mouth hot fill container |
| US6171586B1 (en) * | 1997-06-13 | 2001-01-09 | Genentech, Inc. | Antibody formulation |
| US6172213B1 (en) * | 1997-07-02 | 2001-01-09 | Genentech, Inc. | Anti-IgE antibodies and method of improving polypeptides |
| US5994511A (en) * | 1997-07-02 | 1999-11-30 | Genentech, Inc. | Anti-IgE antibodies and methods of improving polypeptides |
-
2002
- 2002-06-11 EP EP02746513A patent/EP1404813A4/en not_active Withdrawn
- 2002-06-11 US US10/167,517 patent/US20030087372A1/en not_active Abandoned
- 2002-06-11 WO PCT/US2002/018687 patent/WO2002101019A2/en not_active Ceased
- 2002-06-11 JP JP2003503770A patent/JP2004532642A/ja active Pending
- 2002-06-11 AU AU2002316230A patent/AU2002316230A1/en not_active Abandoned
- 2002-06-11 CA CA002447791A patent/CA2447791A1/en not_active Abandoned
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