EA200500695A1 - Процедура получения клеточных линий в безбелковых средах и клеточные линии, полученные этим способом - Google Patents

Процедура получения клеточных линий в безбелковых средах и клеточные линии, полученные этим способом

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Publication number
EA200500695A1
EA200500695A1 EA200500695A EA200500695A EA200500695A1 EA 200500695 A1 EA200500695 A1 EA 200500695A1 EA 200500695 A EA200500695 A EA 200500695A EA 200500695 A EA200500695 A EA 200500695A EA 200500695 A1 EA200500695 A1 EA 200500695A1
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EA
Eurasian Patent Office
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free
protein
present
antibodies
serum
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EA200500695A
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English (en)
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EA007465B1 (ru
Inventor
Роландо Перес Родригес
Витльоч Адольфо Кастильо
Сарасола Свьета Виторес
Тамми Боджано АЕ
Луис Рохас Дель Кальво
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Сентро Де Инмунология Молекулар
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Publication of EA200500695A1 publication Critical patent/EA200500695A1/ru
Publication of EA007465B1 publication Critical patent/EA007465B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Настоящее изобретение относится к способу получения клонов клеток млекопитающих, адаптированных для роста в бессывороточных и безбелковых средах, где используемая процедура включает двустадийный процесс адаптации к росту в таких условиях. Настоящее изобретение относится к интервалу критических концентраций белка, в котором клетки должны выращиваться с тем, чтобы они сохранили способность выживать в бессывороточных и безбелковых условиях, так что в случае клеток, растущих в таком интервале критических концентраций, дальнейшее снижение концентрации не будет сказываться отрицательно ни на жизнеспособности, ни на времени удвоения клеток. Такой интервал критических концентраций является специфичным для конкретной клеточной линии. Кроме того, в настоящем изобретении описаны клоны клеток млекопитающих, которые сохраняют стабильность в бессывороточных и безбелковых средах в течение по меньшей мере 40 генераций; дополнительно, описанные в настоящем изобретении клоны экспрессируют рекомбинантный продукт. Клоны клеток, описанные в настоящем изобретении, продуцируют гуманизированные анти-EGF-R антитела hR3, гуманизированные анти-CD6 антитела T1hT, химерные анти-CD3 антитела T3Q или их фрагменты.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200500695A 2002-10-23 2003-10-22 Процедура получения клеточных линий в безбелковых средах и клеточные линии, полученные этим способом EA007465B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20020239A CU23097A1 (es) 2002-10-23 2002-10-23 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método
PCT/CU2003/000012 WO2004038010A1 (es) 2002-10-23 2003-10-22 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método

Publications (2)

Publication Number Publication Date
EA200500695A1 true EA200500695A1 (ru) 2005-10-27
EA007465B1 EA007465B1 (ru) 2006-10-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA200500695A EA007465B1 (ru) 2002-10-23 2003-10-22 Процедура получения клеточных линий в безбелковых средах и клеточные линии, полученные этим способом

Country Status (27)

Country Link
US (2) US20060036082A1 (ru)
EP (2) EP2363418B1 (ru)
JP (1) JP4674087B2 (ru)
KR (1) KR101186048B1 (ru)
CN (1) CN1714147B (ru)
AR (1) AR041645A1 (ru)
AT (1) ATE502103T1 (ru)
AU (1) AU2003273727B2 (ru)
CA (1) CA2503315C (ru)
CU (1) CU23097A1 (ru)
DE (1) DE60336415D1 (ru)
DK (1) DK1564285T3 (ru)
EA (1) EA007465B1 (ru)
EC (1) ECSP055740A (ru)
ES (2) ES2360049T3 (ru)
GE (1) GEP20084444B (ru)
HK (1) HK1084414A1 (ru)
HR (1) HRP20050760A2 (ru)
LT (1) LT5354B (ru)
LV (1) LV13361B (ru)
MY (1) MY144088A (ru)
NZ (1) NZ540171A (ru)
PE (1) PE20040552A1 (ru)
PT (1) PT1564285E (ru)
TN (1) TNSN05116A1 (ru)
UY (1) UY28037A1 (ru)
WO (1) WO2004038010A1 (ru)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104651314B (zh) * 2015-02-14 2018-06-19 百泰生物药业有限公司 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子
CN104673754B (zh) * 2015-02-14 2018-03-02 百泰生物药业有限公司 重组骨髓瘤细胞克隆的筛选方法及由此获得的抗体分子

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3694263A (en) * 1970-10-23 1972-09-26 Joseph J Korn Sr Swimming pool cleaning methods and apparatus therefor
CU22545A1 (es) 1994-11-18 1999-03-31 Centro Inmunologia Molecular Obtención de un anticuerpo quimérico y humanizado contra el receptor del factor de crecimiento epidérmico para uso diagnóstico y terapéutico
US3886616A (en) * 1972-12-06 1975-06-03 Fay A Hayes Hand propelled swimming pool cleaner
US4275474A (en) * 1979-04-30 1981-06-30 Woodard Randle C Vacuum head for swimming pool cleaning system
US4240174A (en) * 1979-07-30 1980-12-23 Scott Jeffrey L Self-contained mobile pool cleaning apparatus
US4430214A (en) * 1982-09-15 1984-02-07 Baker Marvin E Strainer mill for swimming pool pump intake
AU2986484A (en) * 1983-06-27 1985-01-03 Monash University Leptospira strains grown in protein free medium
US4558479A (en) * 1984-01-26 1985-12-17 Alopex Industries, Inc. Pool cleaner
US4683599A (en) * 1984-03-30 1987-08-04 Fahet Nv Automatic pool cleaner fitting
US4581075A (en) * 1985-03-15 1986-04-08 Maxi-Sweep, Inc. Self-propelled water borne pool cleaner
WO1991005240A1 (en) 1989-09-29 1991-04-18 Atomic Energy Of Canada Limited Infrared-based gas detector
JP2696001B2 (ja) 1991-04-15 1998-01-14 財団法人化学及血清療法研究所 組換え蛋白質産生用培地
EP0531562A1 (de) 1991-09-11 1993-03-17 Doerr, Hans-Wilhelm, Prof. Dr. med. Kultivierung von Säugetierzellen
EP0784684B8 (en) * 1994-09-16 2008-03-26 Cancer Research Institute of Contra Costa RECOMBINANT PEPTIDES DERIVED FROM THE Mc3 ANTI-BA46 ANTIBODY, METHODS OF USE THEREOF, AND METHODS OF HUMANIZING ANTIBODY PEPTIDES
ATE429246T1 (de) 1994-11-10 2009-05-15 Baxter Healthcare Sa Verfahren zur herstellung von biologischen produkten in protein-freiem kultur
CA2211630A1 (en) 1995-02-23 1996-08-29 Quest International B.V. Peptides for tissue and cell culture media
AUPN442295A0 (en) 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
CU22584A1 (es) 1995-11-17 1999-11-03 Centro Inmunologia Molecular Composiciones farmacéuticas que contienen un anticuerpo monoclonal que reconoce el antígeno de diferenciación leucocitario humano cd6 y sus usos para el diagnóstico y tratamiento de la psoriasis
AT407255B (de) * 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons

Also Published As

Publication number Publication date
AU2003273727B2 (en) 2008-04-03
EP1564285B1 (en) 2011-03-16
BR0315565A (pt) 2005-08-23
AU2003273727A1 (en) 2004-05-13
EP2363418B1 (en) 2017-02-08
CU23097A1 (es) 2005-11-18
HK1084414A1 (en) 2006-07-28
JP2006503879A (ja) 2006-02-02
MY144088A (en) 2011-08-15
CA2503315A1 (en) 2004-05-06
LT2005053A (en) 2006-04-25
GEP20084444B (en) 2008-08-10
EA007465B1 (ru) 2006-10-27
PE20040552A1 (es) 2004-10-26
US20100197011A1 (en) 2010-08-05
UY28037A1 (es) 2004-04-30
ECSP055740A (es) 2005-09-20
EP2363418A3 (en) 2011-11-16
BR0315565B1 (pt) 2022-01-25
ATE502103T1 (de) 2011-04-15
KR101186048B1 (ko) 2012-09-25
PT1564285E (pt) 2011-04-20
ES2360049T3 (es) 2011-05-31
AR041645A1 (es) 2005-05-26
LV13361B (en) 2006-02-20
CN1714147A (zh) 2005-12-28
CN1714147B (zh) 2011-01-12
HRP20050760A2 (en) 2006-08-31
LT5354B (lt) 2006-07-25
CA2503315C (en) 2014-02-18
DK1564285T3 (da) 2011-05-16
JP4674087B2 (ja) 2011-04-20
EP2363418A2 (en) 2011-09-07
WO2004038010A1 (es) 2004-05-06
US20060036082A1 (en) 2006-02-16
TNSN05116A1 (en) 2007-05-14
DE60336415D1 (de) 2011-04-28
KR20050088403A (ko) 2005-09-06
EP1564285A1 (en) 2005-08-17
ES2624279T3 (es) 2017-07-13
NZ540171A (en) 2008-04-30

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