CU23097A1 - Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método - Google Patents

Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método

Info

Publication number
CU23097A1
CU23097A1 CU20020239A CU20020239A CU23097A1 CU 23097 A1 CU23097 A1 CU 23097A1 CU 20020239 A CU20020239 A CU 20020239A CU 20020239 A CU20020239 A CU 20020239A CU 23097 A1 CU23097 A1 CU 23097A1
Authority
CU
Cuba
Prior art keywords
present
grow
protein
free
cells
Prior art date
Application number
CU20020239A
Other languages
English (en)
Inventor
Del Calvo Luis Rojas
Ayo Tammy Boggiano
Sarazola Svieta Victores
Vitlloch Adolfo Castillo
Rodriguez Rolando Perez
Original Assignee
Centro Inmunologia Molecular
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Inmunologia Molecular filed Critical Centro Inmunologia Molecular
Priority to CU20020239A priority Critical patent/CU23097A1/es
Priority to PE2003001035A priority patent/PE20040552A1/es
Priority to ARP030103776A priority patent/AR041645A1/es
Priority to MYPI20033959A priority patent/MY144088A/en
Priority to GEAP20038815A priority patent/GEP20084444B/en
Priority to KR1020057006995A priority patent/KR101186048B1/ko
Priority to PT03757654T priority patent/PT1564285E/pt
Priority to JP2004545689A priority patent/JP4674087B2/ja
Priority to NZ540171A priority patent/NZ540171A/en
Priority to AT03757654T priority patent/ATE502103T1/de
Priority to EP11155076.0A priority patent/EP2363418B1/en
Priority to US10/532,269 priority patent/US20060036082A1/en
Priority to DK03757654.3T priority patent/DK1564285T3/da
Priority to CN2003801036918A priority patent/CN1714147B/zh
Priority to ES11155076.0T priority patent/ES2624279T3/es
Priority to BRPI0315565A priority patent/BR0315565B1/pt
Priority to AU2003273727A priority patent/AU2003273727B2/en
Priority to PCT/CU2003/000012 priority patent/WO2004038010A1/es
Priority to EP03757654A priority patent/EP1564285B1/en
Priority to CA2503315A priority patent/CA2503315C/en
Priority to DE60336415T priority patent/DE60336415D1/de
Priority to ES03757654T priority patent/ES2360049T3/es
Priority to EA200500695A priority patent/EA007465B1/ru
Priority to UY28037A priority patent/UY28037A1/es
Priority to EC2005005740A priority patent/ECSP055740A/es
Priority to TNP2005000116A priority patent/TNSN05116A1/en
Priority to LT2005053A priority patent/LT5354B/lt
Priority to LVP-05-61A priority patent/LV13361B/en
Priority to HR20050760A priority patent/HRP20050760A2/xx
Publication of CU23097A1 publication Critical patent/CU23097A1/es
Priority to HK06104515.2A priority patent/HK1084414A1/xx
Priority to US12/759,208 priority patent/US20100197011A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La actual invención se relaciona con un método de obtención de células de mamífero adaptadas a crecer en medio libre de suero y proteínas, el peocedimiento incluye un proceso en dos etapas de adaptación para crecer en esa condición. La actual invención revela la existencia de un intervalo crítico de concentración de proteína en el cual las células deban crecer para ganar la capacidad de sobrevivir en medio libre de proteínas, una vez que las células hayan crecido en las concentraciones críticas del intervalo, las disminuciones subsiguientes de la concentración no afecten la viabilidad ni el tiempo de doblaje celular. El intervalo crítico de la concentración de la proteína es específico para cada línea celular. La presente invención también revela líneas celulares de mamíferos que crecen establemente en condiciones de medio libre de suero y proteínas por al menos 40 generaciones. Adicionalmente, los clones revelados en la presente invenci´´on expresan un producto recombinante. Los clones de la presente invención expresan los anticuerpos anti receptor de EGF hR3, el anti CD6 T1hT humanizados y el anticuerpo quimérico anti CD3 T3Q, además de los fragmentos de dichos anticuerpos.
CU20020239A 2002-10-23 2002-10-23 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método CU23097A1 (es)

Priority Applications (31)

Application Number Priority Date Filing Date Title
CU20020239A CU23097A1 (es) 2002-10-23 2002-10-23 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método
PE2003001035A PE20040552A1 (es) 2002-10-23 2003-10-10 Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo
ARP030103776A AR041645A1 (es) 2002-10-23 2003-10-16 Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo
MYPI20033959A MY144088A (en) 2002-10-23 2003-10-17 Procedure for obtaining cell lines in protein-free media and cell line obtained by the method
BRPI0315565A BR0315565B1 (pt) 2002-10-23 2003-10-22 Método para se obter uma linhagem celular de mieloma nso que contém uma sequência que codifica um polipeptídeo recombinante ou uma proteína recombinante adaptada para crescer em meio livre de soro e proteína, e, uso do método
PCT/CU2003/000012 WO2004038010A1 (es) 2002-10-23 2003-10-22 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método
PT03757654T PT1564285E (pt) 2002-10-23 2003-10-22 Processo para a obtenção de linhas de células de mieoloma de mamífero recombinantes adaptadas para crescer em meio isento de soro e de proteínas
JP2004545689A JP4674087B2 (ja) 2002-10-23 2003-10-22 蛋白質非含有培地中での細胞株の取得方法およびこうして取得された細胞株
NZ540171A NZ540171A (en) 2002-10-23 2003-10-22 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
AT03757654T ATE502103T1 (de) 2002-10-23 2003-10-22 Verfahren zur gewinnung einer rekombinanten säuger- myeloma zelllinie adaptiert an wachstum in serum- und proteinfreien medien
EP11155076.0A EP2363418B1 (en) 2002-10-23 2003-10-22 Method of obtaining recombinant mammalian myeloma cell lines adapted to growth in serum- and protein-free media
US10/532,269 US20060036082A1 (en) 2002-10-23 2003-10-22 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
DK03757654.3T DK1564285T3 (da) 2002-10-23 2003-10-22 Fremgangsmåde til opnåelse af en rekombinant pattedyr myelomacellelinje tilpasset at gro i serum- og proteinfri medier
CN2003801036918A CN1714147B (zh) 2002-10-23 2003-10-22 无蛋白培养基中获得细胞系的方法以及用此方法获得的细胞系
ES11155076.0T ES2624279T3 (es) 2002-10-23 2003-10-22 Procedimiento para obtener líneas celulares de mieloma de mamífero recombinantes adaptadas al crecimiento en medios sin suero ni proteínas
GEAP20038815A GEP20084444B (en) 2002-10-23 2003-10-22 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
AU2003273727A AU2003273727B2 (en) 2002-10-23 2003-10-22 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
KR1020057006995A KR101186048B1 (ko) 2002-10-23 2003-10-22 무단백질 배지에서 세포주의 수득 방법 및 수득된 세포주
EP03757654A EP1564285B1 (en) 2002-10-23 2003-10-22 Method of obtaining a recombinant mammalian myeloma cell lines adapted to growth in serum- and protein-free media
CA2503315A CA2503315C (en) 2002-10-23 2003-10-22 Procedure for obtaining cell lines in a protein-free media and cell line obtained by the method
DE60336415T DE60336415D1 (de) 2002-10-23 2003-10-22 Verfahren zur gewinnung einer rekombinanten säuger- myeloma zelllinie adaptiert an wachstum in serum- und proteinfreien medien
ES03757654T ES2360049T3 (es) 2002-10-23 2003-10-22 Procedimiento para obtener líneas celulares reombinantes de mieloma de mamiífero adaptadas al crecimiento en medios sin suero ni proteínas.
EA200500695A EA007465B1 (ru) 2002-10-23 2003-10-22 Процедура получения клеточных линий в безбелковых средах и клеточные линии, полученные этим способом
UY28037A UY28037A1 (es) 2002-10-23 2003-10-23 Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo
EC2005005740A ECSP055740A (es) 2002-10-23 2005-04-22 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método.
TNP2005000116A TNSN05116A1 (en) 2002-10-23 2005-04-22 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
LT2005053A LT5354B (lt) 2002-10-23 2005-05-19 Ląstelių linijų išskyrimo baltymų neturinčioje terpėje būdas ir šiuo būdu išskirtos ląstelių linijos
LVP-05-61A LV13361B (en) 2002-10-23 2005-05-23 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
HR20050760A HRP20050760A2 (en) 2002-10-23 2005-09-02 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
HK06104515.2A HK1084414A1 (en) 2002-10-23 2006-04-13 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained
US12/759,208 US20100197011A1 (en) 2002-10-23 2010-04-13 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20020239A CU23097A1 (es) 2002-10-23 2002-10-23 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método

Publications (1)

Publication Number Publication Date
CU23097A1 true CU23097A1 (es) 2005-11-18

Family

ID=40134843

Family Applications (1)

Application Number Title Priority Date Filing Date
CU20020239A CU23097A1 (es) 2002-10-23 2002-10-23 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método

Country Status (28)

Country Link
US (2) US20060036082A1 (es)
EP (2) EP2363418B1 (es)
JP (1) JP4674087B2 (es)
KR (1) KR101186048B1 (es)
CN (1) CN1714147B (es)
AR (1) AR041645A1 (es)
AT (1) ATE502103T1 (es)
AU (1) AU2003273727B2 (es)
BR (1) BR0315565B1 (es)
CA (1) CA2503315C (es)
CU (1) CU23097A1 (es)
DE (1) DE60336415D1 (es)
DK (1) DK1564285T3 (es)
EA (1) EA007465B1 (es)
EC (1) ECSP055740A (es)
ES (2) ES2624279T3 (es)
GE (1) GEP20084444B (es)
HK (1) HK1084414A1 (es)
HR (1) HRP20050760A2 (es)
LT (1) LT5354B (es)
LV (1) LV13361B (es)
MY (1) MY144088A (es)
NZ (1) NZ540171A (es)
PE (1) PE20040552A1 (es)
PT (1) PT1564285E (es)
TN (1) TNSN05116A1 (es)
UY (1) UY28037A1 (es)
WO (1) WO2004038010A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104651314B (zh) * 2015-02-14 2018-06-19 百泰生物药业有限公司 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子
CN104673754B (zh) * 2015-02-14 2018-03-02 百泰生物药业有限公司 重组骨髓瘤细胞克隆的筛选方法及由此获得的抗体分子

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Also Published As

Publication number Publication date
LT2005053A (en) 2006-04-25
CN1714147B (zh) 2011-01-12
DE60336415D1 (de) 2011-04-28
PT1564285E (pt) 2011-04-20
EP1564285B1 (en) 2011-03-16
LV13361B (en) 2006-02-20
US20060036082A1 (en) 2006-02-16
ECSP055740A (es) 2005-09-20
ES2624279T3 (es) 2017-07-13
PE20040552A1 (es) 2004-10-26
NZ540171A (en) 2008-04-30
JP2006503879A (ja) 2006-02-02
TNSN05116A1 (en) 2007-05-14
JP4674087B2 (ja) 2011-04-20
EA200500695A1 (ru) 2005-10-27
WO2004038010A1 (es) 2004-05-06
EP2363418B1 (en) 2017-02-08
LT5354B (lt) 2006-07-25
BR0315565A (pt) 2005-08-23
EA007465B1 (ru) 2006-10-27
CN1714147A (zh) 2005-12-28
HRP20050760A2 (en) 2006-08-31
AU2003273727B2 (en) 2008-04-03
US20100197011A1 (en) 2010-08-05
DK1564285T3 (da) 2011-05-16
CA2503315A1 (en) 2004-05-06
ES2360049T3 (es) 2011-05-31
HK1084414A1 (en) 2006-07-28
EP2363418A2 (en) 2011-09-07
KR20050088403A (ko) 2005-09-06
UY28037A1 (es) 2004-04-30
KR101186048B1 (ko) 2012-09-25
AR041645A1 (es) 2005-05-26
GEP20084444B (en) 2008-08-10
AU2003273727A1 (en) 2004-05-13
CA2503315C (en) 2014-02-18
MY144088A (en) 2011-08-15
ATE502103T1 (de) 2011-04-15
BR0315565B1 (pt) 2022-01-25
EP2363418A3 (en) 2011-11-16
EP1564285A1 (en) 2005-08-17

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