CU20170106A7 - Método para obtener alto rendimiento de clones celulares de expresión estable - Google Patents
Método para obtener alto rendimiento de clones celulares de expresión estableInfo
- Publication number
- CU20170106A7 CU20170106A7 CUP2017000106A CU20170106A CU20170106A7 CU 20170106 A7 CU20170106 A7 CU 20170106A7 CU P2017000106 A CUP2017000106 A CU P2017000106A CU 20170106 A CU20170106 A CU 20170106A CU 20170106 A7 CU20170106 A7 CU 20170106A7
- Authority
- CU
- Cuba
- Prior art keywords
- protein
- grow
- recombinant
- clones
- free media
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3076—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
- C07K16/3084—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated gangliosides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/95—Protein-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
<p>La presente invención se refiere a un método para recuperar clones de células de mieloma recombinante adaptadas para crecer en medios libres de proteínas. Este procedimiento comprende líneas de células de mieloma recombinante para las que el proceso de adaptación de media libre de suero a medio libre de proteína no es posible o toma mucho tiempo y se acompaña de Ia pérdida de producción de anticuerpos debido a Ia aparición de poblaciones de células no productoras. Los procedimientos incluyen un proceso de adaptación gradual para crecer en medio libre de proteínas a baja y alta densidad celular. La adaptación fenotípica para crecer en medio libre de proteínas a alta densidad celular fue acompañada por un aumento en Ia velocidad de secreción de Ia molécula de anticuerpo recombinante y en el porcentaje de clones de células productoras estables. Los clones celulares revelados en Ia presente invención producen el anticuerpo recombinante humanizado Anti - NeuGcGM314F7h. Además, los atributos de identidad de dicho anticuerpo recombinante humanizado anti-NeuGcGM3 14F7 se describen en esta invención.</p>
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510080631.3A CN104651314B (zh) | 2015-02-14 | 2015-02-14 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
PCT/CN2016/076135 WO2016127954A1 (zh) | 2015-02-14 | 2016-03-11 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
Publications (1)
Publication Number | Publication Date |
---|---|
CU20170106A7 true CU20170106A7 (es) | 2018-06-05 |
Family
ID=53242962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CUP2017000106A CU20170106A7 (es) | 2015-02-14 | 2016-03-11 | Método para obtener alto rendimiento de clones celulares de expresión estable |
Country Status (17)
Country | Link |
---|---|
US (1) | US10457747B2 (es) |
JP (1) | JP2018506306A (es) |
KR (1) | KR20180029948A (es) |
CN (1) | CN104651314B (es) |
AU (1) | AU2016218641C1 (es) |
BR (1) | BR112017017303B8 (es) |
CA (1) | CA2974027C (es) |
CO (1) | CO2017008087A2 (es) |
CU (1) | CU20170106A7 (es) |
EA (1) | EA201791828A1 (es) |
IL (1) | IL253932A0 (es) |
MX (1) | MX2017010421A (es) |
MY (1) | MY192147A (es) |
NZ (1) | NZ735485A (es) |
SG (1) | SG11201706595TA (es) |
TN (1) | TN2017000302A1 (es) |
WO (1) | WO2016127954A1 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104651314B (zh) * | 2015-02-14 | 2018-06-19 | 百泰生物药业有限公司 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
EP3257935A4 (en) * | 2016-03-11 | 2018-08-08 | Biotech Pharmaceutical Co. Ltd. | Method for obtaining high-yield, stable-expression cell clones and antibody molecules obtained thereby |
CN113480652B (zh) * | 2021-07-30 | 2023-04-07 | 成都景泽生物制药有限公司 | 一种重组cho细胞发酵培养生产重组egfr抗体活性分子的方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CU22731A1 (es) | 1998-02-05 | 2002-02-28 | Centro Inmunologia Molecular | Anticuerpo monoclonal que reconoce el oligosacárido ácido siálico n´glicolilado-galactosa-glucosa (ngcneu-gal-glu) en tumores malignos y composiciones farmacéuticas que los contienen |
GB0208041D0 (en) * | 2002-04-08 | 2002-05-22 | Lonza Biologics Plc | Method of culturing animal cells |
CU23097A1 (es) * | 2002-10-23 | 2005-11-18 | Centro Inmunologia Molecular | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
CU23403A1 (es) * | 2003-04-23 | 2009-08-04 | Centro Inmunologia Molecular | Anticuerpos recombinantes y fragmentos que reconocen el gangliósido n-glicolil gm3 y su uso para diagnóstico y tratamiento de tumores |
AU2004286351A1 (en) * | 2003-11-03 | 2005-05-12 | Centocor, Inc. | Method for maintaining low shear in a bioprocessing system |
CU24120B1 (es) * | 2012-03-01 | 2015-08-27 | Ct De Inmunología Molecular | Anticuerpos recombinantes con especificidad dual por los gangliósidos n-acetil gm3 y n-glicolil gm3 |
CN104152415B (zh) * | 2014-08-13 | 2015-09-30 | 百泰生物药业有限公司 | 获得高产稳定表达重组抗体的骨髓瘤细胞株的方法及应用 |
CN104651314B (zh) * | 2015-02-14 | 2018-06-19 | 百泰生物药业有限公司 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
-
2015
- 2015-02-14 CN CN201510080631.3A patent/CN104651314B/zh active Active
-
2016
- 2016-03-11 MY MYPI2017702967A patent/MY192147A/en unknown
- 2016-03-11 KR KR1020177022618A patent/KR20180029948A/ko not_active Application Discontinuation
- 2016-03-11 CA CA2974027A patent/CA2974027C/en active Active
- 2016-03-11 MX MX2017010421A patent/MX2017010421A/es unknown
- 2016-03-11 US US15/549,087 patent/US10457747B2/en active Active
- 2016-03-11 BR BR112017017303A patent/BR112017017303B8/pt active IP Right Grant
- 2016-03-11 CU CUP2017000106A patent/CU20170106A7/es unknown
- 2016-03-11 WO PCT/CN2016/076135 patent/WO2016127954A1/zh active Application Filing
- 2016-03-11 AU AU2016218641A patent/AU2016218641C1/en active Active
- 2016-03-11 EA EA201791828A patent/EA201791828A1/ru unknown
- 2016-03-11 SG SG11201706595TA patent/SG11201706595TA/en unknown
- 2016-03-11 JP JP2017560865A patent/JP2018506306A/ja active Pending
- 2016-03-11 TN TNP/2017/000302A patent/TN2017000302A1/en unknown
- 2016-03-11 NZ NZ735485A patent/NZ735485A/en active IP Right Revival
-
2017
- 2017-08-09 IL IL253932A patent/IL253932A0/en unknown
- 2017-08-10 CO CONC2017/0008087A patent/CO2017008087A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
AU2016218641A1 (en) | 2017-10-05 |
MX2017010421A (es) | 2018-04-26 |
TN2017000302A1 (en) | 2019-01-16 |
CA2974027C (en) | 2021-02-16 |
SG11201706595TA (en) | 2017-09-28 |
BR112017017303B8 (pt) | 2024-03-05 |
US10457747B2 (en) | 2019-10-29 |
AU2016218641C1 (en) | 2020-02-27 |
CA2974027A1 (en) | 2016-08-18 |
BR112017017303B1 (pt) | 2023-08-08 |
NZ735485A (en) | 2019-08-30 |
AU2016218641B2 (en) | 2019-10-31 |
CO2017008087A2 (es) | 2017-10-10 |
US20180016353A1 (en) | 2018-01-18 |
EA201791828A1 (ru) | 2017-12-29 |
MY192147A (en) | 2022-08-01 |
WO2016127954A1 (zh) | 2016-08-18 |
IL253932A0 (en) | 2017-10-31 |
KR20180029948A (ko) | 2018-03-21 |
JP2018506306A (ja) | 2018-03-08 |
CN104651314A (zh) | 2015-05-27 |
CN104651314B (zh) | 2018-06-19 |
BR112017017303A2 (pt) | 2018-04-10 |
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