CO2017008087A2 - Método para obtener clones celulares de expresión estable de alto rendimiento y moléculas de anticuerpos obtenidas de este modo - Google Patents

Método para obtener clones celulares de expresión estable de alto rendimiento y moléculas de anticuerpos obtenidas de este modo

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Publication number
CO2017008087A2
CO2017008087A2 CONC2017/0008087A CO2017008087A CO2017008087A2 CO 2017008087 A2 CO2017008087 A2 CO 2017008087A2 CO 2017008087 A CO2017008087 A CO 2017008087A CO 2017008087 A2 CO2017008087 A2 CO 2017008087A2
Authority
CO
Colombia
Prior art keywords
recombinant
protein
cell clones
free medium
antibody
Prior art date
Application number
CONC2017/0008087A
Other languages
English (en)
Inventor
Meylen Chea
Julio Palacios
Miguel Arias
Loany Calvo
Tamara Gonzalez
Rolando Perez
zhi Bai
Yuemao Liu
Kaiheng Xiao
Xiao Chen
Zhenhua He
Yangliu Cai
Zhenhua Yang
Xianhong Bai
Original Assignee
Biotech Pharmaceutical Co Ltd
Ct Inmunologia Molecular
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotech Pharmaceutical Co Ltd, Ct Inmunologia Molecular filed Critical Biotech Pharmaceutical Co Ltd
Publication of CO2017008087A2 publication Critical patent/CO2017008087A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3076—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
    • C07K16/3084—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated gangliosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a un método para recuperar clones de células de mieloma recombinante adaptadas para crecer en medios libres de proteínas. Este procedimiento comprende líneas de células de mieloma recombinante para las que el proceso de adaptación de medio libre de suero a medio libre de proteína no es posible o toma mucho tiempo y se acompaña de la pérdida de producción de anticuerpos debido a la aparición de poblaciones de células no productoras. Los procedimientos incluyen un proceso de adaptación gradual para crecer en medio libre de proteínas a baja y alta densidad celular. La adaptación fenotípica para crecer en medio libre de proteínas a alta densidad celular fue acompañada por un aumento en la velocidad de secreción de la molécula de anticuerpo recombinante y en el porcentaje de clones de células productoras estables. Los clones celulares revelados en la presente invención producen el anticuerpo recombinante humanizado Anti - NeuGcGM3 14F7h. Además, los atributos de identidad de dicho anticuerpo recombinante humanizado anti-NeuGcGM3 14F7 se describen en esta invención.
CONC2017/0008087A 2015-02-14 2017-08-10 Método para obtener clones celulares de expresión estable de alto rendimiento y moléculas de anticuerpos obtenidas de este modo CO2017008087A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510080631.3A CN104651314B (zh) 2015-02-14 2015-02-14 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子
PCT/CN2016/076135 WO2016127954A1 (zh) 2015-02-14 2016-03-11 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子

Publications (1)

Publication Number Publication Date
CO2017008087A2 true CO2017008087A2 (es) 2017-10-10

Family

ID=53242962

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2017/0008087A CO2017008087A2 (es) 2015-02-14 2017-08-10 Método para obtener clones celulares de expresión estable de alto rendimiento y moléculas de anticuerpos obtenidas de este modo

Country Status (17)

Country Link
US (1) US10457747B2 (es)
JP (1) JP2018506306A (es)
KR (1) KR20180029948A (es)
CN (1) CN104651314B (es)
AU (1) AU2016218641C1 (es)
BR (1) BR112017017303B8 (es)
CA (1) CA2974027C (es)
CO (1) CO2017008087A2 (es)
CU (1) CU20170106A7 (es)
EA (1) EA201791828A1 (es)
IL (1) IL253932A0 (es)
MX (1) MX2017010421A (es)
MY (1) MY192147A (es)
NZ (1) NZ735485A (es)
SG (1) SG11201706595TA (es)
TN (1) TN2017000302A1 (es)
WO (1) WO2016127954A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104651314B (zh) * 2015-02-14 2018-06-19 百泰生物药业有限公司 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子
EP3257935A4 (en) * 2016-03-11 2018-08-08 Biotech Pharmaceutical Co. Ltd. Method for obtaining high-yield, stable-expression cell clones and antibody molecules obtained thereby
CN113480652B (zh) * 2021-07-30 2023-04-07 成都景泽生物制药有限公司 一种重组cho细胞发酵培养生产重组egfr抗体活性分子的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU22731A1 (es) 1998-02-05 2002-02-28 Centro Inmunologia Molecular Anticuerpo monoclonal que reconoce el oligosacárido ácido siálico n´glicolilado-galactosa-glucosa (ngcneu-gal-glu) en tumores malignos y composiciones farmacéuticas que los contienen
GB0208041D0 (en) * 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
CU23097A1 (es) * 2002-10-23 2005-11-18 Centro Inmunologia Molecular Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método
CU23403A1 (es) * 2003-04-23 2009-08-04 Centro Inmunologia Molecular Anticuerpos recombinantes y fragmentos que reconocen el gangliósido n-glicolil gm3 y su uso para diagnóstico y tratamiento de tumores
AU2004286351A1 (en) * 2003-11-03 2005-05-12 Centocor, Inc. Method for maintaining low shear in a bioprocessing system
CU24120B1 (es) * 2012-03-01 2015-08-27 Ct De Inmunología Molecular Anticuerpos recombinantes con especificidad dual por los gangliósidos n-acetil gm3 y n-glicolil gm3
CN104152415B (zh) * 2014-08-13 2015-09-30 百泰生物药业有限公司 获得高产稳定表达重组抗体的骨髓瘤细胞株的方法及应用
CN104651314B (zh) * 2015-02-14 2018-06-19 百泰生物药业有限公司 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子

Also Published As

Publication number Publication date
AU2016218641A1 (en) 2017-10-05
MX2017010421A (es) 2018-04-26
TN2017000302A1 (en) 2019-01-16
CA2974027C (en) 2021-02-16
SG11201706595TA (en) 2017-09-28
BR112017017303B8 (pt) 2024-03-05
US10457747B2 (en) 2019-10-29
AU2016218641C1 (en) 2020-02-27
CA2974027A1 (en) 2016-08-18
CU20170106A7 (es) 2018-06-05
BR112017017303B1 (pt) 2023-08-08
NZ735485A (en) 2019-08-30
AU2016218641B2 (en) 2019-10-31
US20180016353A1 (en) 2018-01-18
EA201791828A1 (ru) 2017-12-29
MY192147A (en) 2022-08-01
WO2016127954A1 (zh) 2016-08-18
IL253932A0 (en) 2017-10-31
KR20180029948A (ko) 2018-03-21
JP2018506306A (ja) 2018-03-08
CN104651314A (zh) 2015-05-27
CN104651314B (zh) 2018-06-19
BR112017017303A2 (pt) 2018-04-10

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