CO2022007349A2 - Métodos para producir células de epitelio pigmentario retiniano - Google Patents

Métodos para producir células de epitelio pigmentario retiniano

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Publication number
CO2022007349A2
CO2022007349A2 CONC2022/0007349A CO2022007349A CO2022007349A2 CO 2022007349 A2 CO2022007349 A2 CO 2022007349A2 CO 2022007349 A CO2022007349 A CO 2022007349A CO 2022007349 A2 CO2022007349 A2 CO 2022007349A2
Authority
CO
Colombia
Prior art keywords
retinal pigment
pigment epithelial
methods
epithelial cells
producing retinal
Prior art date
Application number
CONC2022/0007349A
Other languages
English (en)
Inventor
Yasuhiro Takagi
Meng-Jiao Shi
Mi Sook Chang
Irina Klimanskaya
Original Assignee
Astellas Inst For Regenerative Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astellas Inst For Regenerative Medicine filed Critical Astellas Inst For Regenerative Medicine
Publication of CO2022007349A2 publication Critical patent/CO2022007349A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Ophthalmology & Optometry (AREA)
  • Wood Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

La presente invención proporciona un método mejorado para producir células de epitelio pigmentario retiniano (RPE) altamente puras mediante diferenciación de células madre pluripotentes.
CONC2022/0007349A 2019-10-30 2022-05-27 Métodos para producir células de epitelio pigmentario retiniano CO2022007349A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962928125P 2019-10-30 2019-10-30
PCT/US2020/057654 WO2021086911A1 (en) 2019-10-30 2020-10-28 Methods for producing retinal pigment epithelium cells

Publications (1)

Publication Number Publication Date
CO2022007349A2 true CO2022007349A2 (es) 2022-08-30

Family

ID=73476265

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2022/0007349A CO2022007349A2 (es) 2019-10-30 2022-05-27 Métodos para producir células de epitelio pigmentario retiniano

Country Status (13)

Country Link
US (1) US20230072771A1 (es)
EP (1) EP4051785A1 (es)
JP (1) JP2023500830A (es)
KR (1) KR20220106965A (es)
CN (1) CN114981417A (es)
AU (1) AU2020374884A1 (es)
BR (1) BR112022006644A2 (es)
CA (1) CA3158763A1 (es)
CO (1) CO2022007349A2 (es)
IL (1) IL292610A (es)
MX (1) MX2022005134A (es)
TW (1) TW202130806A (es)
WO (1) WO2021086911A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230058544A (ko) 2015-08-18 2023-05-03 아스텔라스 인스티튜트 포 리제너러티브 메디슨 임상 제제
US11371016B2 (en) * 2016-04-22 2022-06-28 Sumitomo Pharma Co., Ltd. Method for producing retinal tissue

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7794704B2 (en) 2004-01-23 2010-09-14 Advanced Cell Technology, Inc. Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
AU2012201562B2 (en) * 2004-01-23 2013-07-04 President And Fellows Of Harvard College Improved modalities for the treatment of degenerative diseases of the retina
EP2438814A3 (en) 2004-01-23 2012-10-03 Advanced Cell Technology, Inc. Improved modalities for the treatment of degenerative diseases of the retina
EP2479256A1 (en) 2004-11-04 2012-07-25 Advanced Cell Technology, Inc. Derivation of embryonic stem cells
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
PL2185691T3 (pl) 2007-07-31 2018-08-31 Lifescan, Inc. Różnicowanie pluripotencjalnych komórek macierzystych poprzez zastosowanie ludzkich komórek odżywczych
CA2702386C (en) * 2007-10-12 2018-07-24 Advanced Cell Technology, Inc. Improved methods of producing rpe cells and compositions of rpe cells
KR102073730B1 (ko) 2009-11-17 2020-02-05 아스텔라스 인스티튜트 포 리제너러티브 메디슨 인간 ｒｐｅ 세포의 생산 방법 및 인간 ｒｐｅ 세포의 제약 제제
ES2542226T3 (es) * 2010-04-28 2015-08-03 Technische Universität Dresden Método para producir células progenitoras retinianas polarizadas a partir de células madre pluripotentes y su diferenciación para dar células del epitelio pigmentario retiniano
JP6184865B2 (ja) 2010-07-23 2017-08-23 アステラス インスティテュート フォー リジェネレイティブ メディシン 細胞の稀少なサブ集団を検出するための方法及び細胞の高度に精製された組成物
DK2694644T3 (en) 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming of pluripotent stem cells for neural differentiation
TWI719277B (zh) 2011-11-14 2021-02-21 美商安斯泰來再生醫藥協會 人類rpe細胞之醫藥組合物及其用途
WO2014121077A2 (en) 2013-02-01 2014-08-07 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services METHOD FOR GENERATING RETINAL PIGMENT EPITHELIUM (RPE) CELLS FROM INDUCED PLURIPOTENT STEM CELLS (IPSCs)
SG11201602796QA (en) * 2013-10-09 2016-05-30 Healios Kk Method for purification of retinal pigment epithelial cells
WO2015175504A1 (en) * 2014-05-12 2015-11-19 The Johns Hopkins University Differentiation of human pluripotent stem cells into retinal pigment epithelium using hif1 inhibitors
US11422125B2 (en) 2015-03-23 2022-08-23 Astellas Institute For Regenerative Medicine Assays for potency of human retinal pigment epithelium (RPE) cells and photoreceptor progenitors
KR20230058544A (ko) 2015-08-18 2023-05-03 아스텔라스 인스티튜트 포 리제너러티브 메디슨 임상 제제
EP3347456B1 (en) * 2015-09-08 2023-12-27 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
CN108291206B (zh) 2015-09-08 2022-07-08 富士胶片细胞动力公司 干细胞来源的视网膜色素上皮的基于macs的纯化
US20180312805A1 (en) * 2015-10-26 2018-11-01 Cell Cure Neurosciences Ltd. Preparation of Retinal Pigment Epithelium Cells
CN108728413A (zh) * 2017-06-13 2018-11-02 中山大学中山眼科中心 一种人多能干细胞来源人视网膜色素上皮细胞的制备和扩增培养方法
JP7498664B2 (ja) 2017-12-29 2024-06-12 セル キュア ニューロサイエンシズ リミテッド 網膜色素上皮細胞組成物
CN108531443A (zh) * 2018-04-04 2018-09-14 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 小分子诱导多能性干细胞分化为视网膜色素上皮细胞的方法

Also Published As

Publication number Publication date
WO2021086911A1 (en) 2021-05-06
TW202130806A (zh) 2021-08-16
AU2020374884A1 (en) 2022-06-09
EP4051785A1 (en) 2022-09-07
BR112022006644A2 (pt) 2022-07-12
CA3158763A1 (en) 2021-05-06
KR20220106965A (ko) 2022-08-01
MX2022005134A (es) 2022-05-30
CN114981417A (zh) 2022-08-30
US20230072771A1 (en) 2023-03-09
IL292610A (en) 2022-07-01
JP2023500830A (ja) 2023-01-11

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