CN113480652B - 一种重组cho细胞发酵培养生产重组egfr抗体活性分子的方法 - Google Patents
一种重组cho细胞发酵培养生产重组egfr抗体活性分子的方法 Download PDFInfo
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Abstract
Description
培养基 | 组分 | 实验例2 | 实验例3 | 实验例4 |
第一培养基 | CDM4PERMab | |||
第一培养基 | L-Gln(mM/L) | 6 | 4 | 8 |
第一培养基 | Sheff-CHO PF ACF(g/L) | 3 | 2 | 5 |
第一培养基 | 泊洛沙姆188(%) | 0.2 | 0.2 | 0.2 |
第二培养基 | 2*103A高效补料培养基 | |||
第二培养基 | Sheff-CHO PF ACF(g/L) | 50 | 80 | 20 |
L-胱氨酸二盐酸盐(mg/L) | 50 | 40 | 80 |
Claims (5)
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CN114195904B (zh) * | 2021-12-27 | 2022-12-06 | 成都蓉生药业有限责任公司 | 一种生产长效重组人凝血因子viii的重组细胞的分批补料培养方法 |
CN114317392A (zh) * | 2021-12-27 | 2022-04-12 | 无锡药明生物技术股份有限公司 | 优化细胞培养的方法 |
CN114480492B (zh) * | 2022-01-28 | 2023-04-21 | 景泽生物医药(合肥)股份有限公司 | 一种重组人抗体融合蛋白的制备方法 |
CN116063564A (zh) * | 2022-10-08 | 2023-05-05 | 盛禾(中国)生物制药有限公司 | 一种纯化融合蛋白的方法 |
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CN101932591A (zh) * | 2007-07-09 | 2010-12-29 | 健泰科生物技术公司 | 在多肽的重组生产期间防止二硫键还原 |
CN108220334A (zh) * | 2016-12-09 | 2018-06-29 | 上海迈泰君奥生物技术有限公司 | 无血清细胞培养基及高效表达重组蛋白质的方法 |
CN108611342A (zh) * | 2018-03-27 | 2018-10-02 | 成都蓉生药业有限责任公司 | 一种重组人凝血因子ix活性分子的补料发酵方法 |
CN110229774A (zh) * | 2019-07-02 | 2019-09-13 | 山东国力生物科技有限公司 | 一种精氨酸脱羧酶基因工程菌及其高密度发酵培养方法 |
WO2020227121A1 (en) * | 2019-05-03 | 2020-11-12 | Coherus Biosciences, Inc. | Method of producing a recombinant protein |
WO2021133084A1 (ko) * | 2019-12-24 | 2021-07-01 | 주식회사 엘지화학 | 베로 세포 배양용 저혈청 배지 조성물 및 이의 이용 |
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CN104651314B (zh) * | 2015-02-14 | 2018-06-19 | 百泰生物药业有限公司 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
TWI797060B (zh) * | 2015-08-04 | 2023-04-01 | 美商再生元醫藥公司 | 補充牛磺酸之細胞培養基及用法 |
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CN101932591A (zh) * | 2007-07-09 | 2010-12-29 | 健泰科生物技术公司 | 在多肽的重组生产期间防止二硫键还原 |
CN108220334A (zh) * | 2016-12-09 | 2018-06-29 | 上海迈泰君奥生物技术有限公司 | 无血清细胞培养基及高效表达重组蛋白质的方法 |
CN108611342A (zh) * | 2018-03-27 | 2018-10-02 | 成都蓉生药业有限责任公司 | 一种重组人凝血因子ix活性分子的补料发酵方法 |
WO2020227121A1 (en) * | 2019-05-03 | 2020-11-12 | Coherus Biosciences, Inc. | Method of producing a recombinant protein |
CN110229774A (zh) * | 2019-07-02 | 2019-09-13 | 山东国力生物科技有限公司 | 一种精氨酸脱羧酶基因工程菌及其高密度发酵培养方法 |
WO2021133084A1 (ko) * | 2019-12-24 | 2021-07-01 | 주식회사 엘지화학 | 베로 세포 배양용 저혈청 배지 조성물 및 이의 이용 |
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