CY1106111T1 - Ανασυνδυασμενος σταθepος κυτταρικος κλωνος, η παρασκευη και χρησιμοποιηση του - Google Patents
Ανασυνδυασμενος σταθepος κυτταρικος κλωνος, η παρασκευη και χρησιμοποιηση τουInfo
- Publication number
- CY1106111T1 CY1106111T1 CY20061100911T CY061100911T CY1106111T1 CY 1106111 T1 CY1106111 T1 CY 1106111T1 CY 20061100911 T CY20061100911 T CY 20061100911T CY 061100911 T CY061100911 T CY 061100911T CY 1106111 T1 CY1106111 T1 CY 1106111T1
- Authority
- CY
- Cyprus
- Prior art keywords
- cell clone
- protein
- preparation
- stable
- recombinant
- Prior art date
Links
- 239000002028 Biomass Substances 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/005—Protein-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Περιγράφονται ένας σταθερός ανασυνδυασμένος κυτταρικός κλώνος, ο οποίος είναι σταθερός για τουλάχιστον 40 γενιές σε ελεύθερο ορού και πρωτεϊνών μέσο, μία βιομάζα που προήλθε από τον πολλαπλασιασμό του σταθερού κυτταρικού κλώνου σε συνθήκες καλλιέργειας ελεύθερες ορού και πρωτεϊνών και μία μέθοδος παρασκευής ανασυνδυασμένων πρωτεϊνών μέσω της βιομάζας. Επιπλέον, η εφεύρεση αφορά μία μέθοδο παραγωγής σταθερών ανασυνδυασμένων κυτταρικών κλώνων. Η εφεύρεση αφορά περαιτέρω την παρασκευή μιας ανασυνδυασμένης πρωτεΐνης σε ένα συνθετικό ελάχιστο μέσο ελεύθερο ορού και πρωτεϊνών.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/AT1999/000197 WO2001011021A1 (de) | 1999-08-05 | 1999-08-05 | Rekombinanter stabiler zellklon, seine herstellung und verwendung |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1106111T1 true CY1106111T1 (el) | 2011-06-08 |
Family
ID=3683861
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20061100911T CY1106111T1 (el) | 1999-08-05 | 2006-07-03 | Ανασυνδυασμενος σταθepος κυτταρικος κλωνος, η παρασκευη και χρησιμοποιηση του |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP1200561B1 (el) |
JP (1) | JP2003506077A (el) |
AT (1) | ATE330003T1 (el) |
AU (1) | AU780810B2 (el) |
CA (1) | CA2381173A1 (el) |
CY (1) | CY1106111T1 (el) |
DE (1) | DE59913565D1 (el) |
DK (1) | DK1200561T3 (el) |
ES (1) | ES2267280T3 (el) |
PT (1) | PT1200561E (el) |
WO (1) | WO2001011021A1 (el) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU4330597A (en) | 1996-08-30 | 1998-03-19 | Life Technologies, Inc. | Serum-free mammalian cell culture medium, and uses thereof |
US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
AT409379B (de) | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
BR0114374A (pt) | 2000-10-02 | 2003-12-30 | Novo Nordisk As | Preparação compreendendo uma pluralidade de polipeptìdeos de fator vii, métodos para a determinação do padrão de glicoforma de fator vii e de polipeptìdeos relacionados com fator vii, e para a produção da dita preparação, formulação farmacêutica, métodos para o tratamento de uma sìndrome responsiva a fator vii, para a prevenção de sangramento indesejado, para a prevenção de coagulação sanguìnea indesejada, e para prevenção de reações mediadas por fator de tecido, e, uso da preparação |
CN1309825C (zh) | 2001-10-02 | 2007-04-11 | 诺和诺德医疗保健公司 | 在真核细胞中生产重组蛋白的方法 |
KR20040065231A (ko) * | 2001-11-28 | 2004-07-21 | 산도즈 게엠베하 | 세포 배양 방법 |
AU2002354363A1 (en) * | 2001-12-04 | 2003-06-17 | Mitsubishi Pharma Corporation | Method of activating protein |
JP2005532057A (ja) | 2002-07-09 | 2005-10-27 | バクスター・インターナショナル・インコーポレイテッド | 細胞を培養するための動物性タンパク質非含有培地 |
US8669109B2 (en) | 2003-08-19 | 2014-03-11 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Methods of producing proteins in Chinese hamster ovary (CHO) cells |
DE10338531A1 (de) * | 2003-08-19 | 2005-04-07 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Verfahren zur Reklonierung von Produktionszellen |
AU2004309063B2 (en) * | 2003-12-31 | 2010-10-28 | Merck Patent Gmbh | Fc-erythropoietin fusion protein with improved pharmacokinetics |
AU2011221414B2 (en) * | 2004-10-29 | 2012-09-20 | Takeda Pharmaceutical Company Limited | Animal Protein-Free Media for Cultivation of Cells |
US20060094104A1 (en) | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
JP5523674B2 (ja) * | 2005-02-11 | 2014-06-18 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 植物タンパク質加水分解産物を含有する血清フリーの細胞培養液におけるポリペプチドの生産 |
EP2522717B1 (en) | 2006-01-04 | 2014-04-02 | Baxter International Inc | Oligopeptide-free cell culture media |
HUE026210T2 (en) * | 2008-11-12 | 2016-05-30 | Baxalta Inc | Procedure for serum-free, insulin-free VII. factor |
WO2024024671A1 (ja) * | 2022-07-27 | 2024-02-01 | 不二製油グループ本社株式会社 | 動物細胞増殖促進剤 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
DE4313620A1 (de) * | 1993-04-26 | 1994-10-27 | Biotechnolog Forschung Gmbh | Hamsterzellinien und Verfahren zur Glykoproteingewinnung |
EP0666312A1 (en) * | 1994-02-08 | 1995-08-09 | Wolfgang A. Renner | Process for the improvement of mammalian cell growth |
WO1996015231A2 (en) * | 1994-11-10 | 1996-05-23 | Immuno Aktiengesellschaft | Method for producing biologicals in protein-free culture |
AU4302796A (en) * | 1994-12-16 | 1996-07-03 | Novartis Ag | Production of recombinant secretory component |
AUPN442295A0 (en) * | 1995-07-26 | 1995-08-17 | Commonwealth Scientific And Industrial Research Organisation | Regulated autocrine growth of mammalian cells |
US5851800A (en) * | 1996-05-14 | 1998-12-22 | Pharmacia & Upjohn Ab | Process for producing a protein |
-
1999
- 1999-08-05 AU AU53636/99A patent/AU780810B2/en not_active Expired
- 1999-08-05 EP EP99939251A patent/EP1200561B1/de not_active Revoked
- 1999-08-05 CA CA 2381173 patent/CA2381173A1/en not_active Abandoned
- 1999-08-05 PT PT99939251T patent/PT1200561E/pt unknown
- 1999-08-05 WO PCT/AT1999/000197 patent/WO2001011021A1/de active IP Right Grant
- 1999-08-05 DK DK99939251T patent/DK1200561T3/da active
- 1999-08-05 AT AT99939251T patent/ATE330003T1/de active
- 1999-08-05 ES ES99939251T patent/ES2267280T3/es not_active Expired - Lifetime
- 1999-08-05 DE DE59913565T patent/DE59913565D1/de not_active Expired - Lifetime
- 1999-08-05 JP JP2001515808A patent/JP2003506077A/ja not_active Withdrawn
-
2006
- 2006-07-03 CY CY20061100911T patent/CY1106111T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
DK1200561T3 (da) | 2006-10-16 |
DE59913565D1 (de) | 2006-07-27 |
EP1200561A1 (de) | 2002-05-02 |
WO2001011021A1 (de) | 2001-02-15 |
AU5363699A (en) | 2001-03-05 |
CA2381173A1 (en) | 2001-02-15 |
PT1200561E (pt) | 2006-09-29 |
ATE330003T1 (de) | 2006-07-15 |
AU780810B2 (en) | 2005-04-21 |
ES2267280T3 (es) | 2007-03-01 |
EP1200561B1 (de) | 2006-06-14 |
JP2003506077A (ja) | 2003-02-18 |
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