JP2004520049A5 - - Google Patents

Download PDF

Info

Publication number
JP2004520049A5
JP2004520049A5 JP2002561621A JP2002561621A JP2004520049A5 JP 2004520049 A5 JP2004520049 A5 JP 2004520049A5 JP 2002561621 A JP2002561621 A JP 2002561621A JP 2002561621 A JP2002561621 A JP 2002561621A JP 2004520049 A5 JP2004520049 A5 JP 2004520049A5
Authority
JP
Japan
Prior art keywords
propeptide sequence
recombinant
nucleic acid
trypsinogen
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2002561621A
Other languages
English (en)
Japanese (ja)
Other versions
JP4159878B2 (ja
JP2004520049A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/EP2002/001072 external-priority patent/WO2002061064A2/de
Publication of JP2004520049A publication Critical patent/JP2004520049A/ja
Publication of JP2004520049A5 publication Critical patent/JP2004520049A5/ja
Application granted granted Critical
Publication of JP4159878B2 publication Critical patent/JP4159878B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

JP2002561621A 2001-02-01 2002-02-01 組換えトリプシンの製造方法 Expired - Lifetime JP4159878B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01102342 2001-02-01
PCT/EP2002/001072 WO2002061064A2 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Publications (3)

Publication Number Publication Date
JP2004520049A JP2004520049A (ja) 2004-07-08
JP2004520049A5 true JP2004520049A5 (enExample) 2005-07-07
JP4159878B2 JP4159878B2 (ja) 2008-10-01

Family

ID=8176376

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002561621A Expired - Lifetime JP4159878B2 (ja) 2001-02-01 2002-02-01 組換えトリプシンの製造方法

Country Status (10)

Country Link
US (2) US7276605B2 (enExample)
EP (1) EP1399568B1 (enExample)
JP (1) JP4159878B2 (enExample)
CN (1) CN1545553B (enExample)
AT (1) ATE310092T1 (enExample)
CA (1) CA2437342C (enExample)
CZ (1) CZ303658B6 (enExample)
DE (1) DE50204952D1 (enExample)
DK (1) DK1399568T3 (enExample)
WO (1) WO2002061064A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002061064A2 (de) * 2001-02-01 2002-08-08 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin
DK1546302T3 (da) 2002-08-30 2009-12-14 Novozymes Inc Fremgangsmåder til fremstilling af pattedyrs-trypsiner
ES2370657T3 (es) * 2005-09-14 2011-12-21 Sanofi-Aventis Deutschland Gmbh Escisión de precursores de insulinas mediante una variante de tripsina.
AU2009336710A1 (en) * 2009-01-06 2011-06-30 Nestec S.A. Processing of macronutrients
CN101967469B (zh) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 一种高稳定性的重组胰蛋白酶的生产及应用
CN102482675B (zh) * 2009-09-10 2015-04-29 拜康有限公司 新型脂酶原-牛胰蛋白酶原融合蛋白
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN103805584B (zh) * 2012-11-12 2016-08-24 宜昌东阳光长江药业股份有限公司 一种重组胰蛋白酶纯化方法
CN103405756B (zh) * 2013-07-10 2014-12-24 浙江众益制药股份有限公司 药物组合物复方消化酶胶囊(ii)及其制备方法
MA39835A (fr) 2014-04-17 2017-02-22 Biogen Ma Inc Compositions et procédés de modulation de l'épissage du smn2 chez un patient
CN104046605A (zh) * 2014-05-29 2014-09-17 中国科学院广州能源研究所 一种嗜温耐乙醇β-葡萄糖苷酶及其编码基因和应用
US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
JP6686361B2 (ja) * 2014-11-26 2020-04-22 東ソー株式会社 細胞回収のための標本作製方法
WO2016164896A2 (en) 2015-04-10 2016-10-13 Ionis Pharmaceuticals, Inc. Modulation of smn expression
WO2017218884A1 (en) 2016-06-16 2017-12-21 Ionis Pharmaceuticals, Inc. Combinations for the modulation of smn expression
CN106350497B (zh) * 2016-11-24 2019-07-16 天津大学 一种胰蛋白酶及其制备方法与应用
CN110511916B (zh) * 2019-08-06 2021-06-01 杭州浦泰生物科技有限公司 一种重组胰蛋白酶的生产工艺
AR121446A1 (es) 2020-02-28 2022-06-08 Ionis Pharmaceuticals Inc Compuestos y métodos para modular smn2
CN115216463B (zh) * 2022-06-15 2023-08-15 武汉瀚海新酶生物科技有限公司 具有稳定性的重组胰蛋白酶及其制备方法和应用
CN120225684A (zh) 2022-11-23 2025-06-27 豪夫迈·罗氏有限公司 用于增加重组蛋白表达的方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE249282C (enExample)
US4851341A (en) * 1986-12-19 1989-07-25 Immunex Corporation Immunoaffinity purification system
US5011912A (en) * 1986-12-19 1991-04-30 Immunex Corporation Hybridoma and monoclonal antibody for use in an immunoaffinity purification system
US7247453B1 (en) * 1988-12-30 2007-07-24 Oklahoma Medical Research Foundation Calcium binding recombinant antibody against protein C
US5298599A (en) * 1988-12-30 1994-03-29 Oklahoma Medical Research Foundation Expression and purification of recombinant soluble tissue factor
US6433142B1 (en) * 1989-08-08 2002-08-13 Genetics Institute, Llc Megakaryocyte stimulating factors
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
CA2102673A1 (en) 1992-11-13 1994-05-14 Michael G. Greaney Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith
US5472692A (en) * 1993-07-02 1995-12-05 New England Deaconess Hospital Corporation Pro-urokinase mutants
CA2157219C (en) * 1994-08-31 2010-10-05 Munehiro Noda Process for purifying recombinant human serum albumin
US5760189A (en) * 1995-06-02 1998-06-02 Genetics Institute, Inc. Protein recovery & purification methods
EP0833898A1 (en) 1995-06-16 1998-04-08 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
CN1187849A (zh) * 1995-06-16 1998-07-15 诺沃挪第克公司 产生胰蛋白酶(胰蛋白酶原)的方法
US5945328A (en) 1995-06-16 1999-08-31 Novo Nordisk A/S Process for producing trypsin (trypsinogen)
ATE331798T1 (de) * 1996-04-19 2006-07-15 Jackson H M Found Military Med Verfahren zur anregung einer immunantwort durch verabreichung von nutzorganismen, die intimin allein oder als fusionsprotein mit einem oder mehreren anderen antigenen exprimieren
GB9609702D0 (en) * 1996-05-09 1996-07-10 Royal Free Hosp School Med Anticoagulant peptides
JP4515542B2 (ja) * 1997-02-10 2010-08-04 ジェネンテック， インコーポレイテッド ヒレグリン変異体
IL134653A0 (en) 1997-08-22 2001-04-30 Roche Diagnostics Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
US20030207402A1 (en) 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
US6159722A (en) * 1997-12-03 2000-12-12 Boehringer Mannheim Gmbh Chimeric serine proteases
US6486303B1 (en) * 1998-04-14 2002-11-26 University Of Medicine & Dentistry Of New Jersey Method for making hormone heterodimers
US6087558A (en) * 1998-07-22 2000-07-11 Prodigene, Inc. Commercial production of proteases in plants
AU6388499A (en) * 1998-09-21 2000-04-10 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
ES2265693T3 (es) * 1998-10-16 2007-02-16 Biogen Idec Ma Inc. Proteinas de fusion con interferon-beta y usos.
EP1132479B1 (en) * 1998-11-20 2009-04-22 Fuso Pharmaceutical Industries Ltd. Protein expression vector and utilization thereof
US20020102709A1 (en) * 1999-02-19 2002-08-01 Tetsuya Ishikawa Collagen-binding physiologically active polypeptide
US6485957B1 (en) * 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6458564B1 (en) * 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
AU7053500A (en) * 1999-09-15 2001-04-17 Eli Lilly And Company Chymotrypsin-free trypsin
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
WO2001055429A2 (en) * 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
CA2408630A1 (en) * 2000-06-19 2001-12-27 Dyax Corp. Novel enterokinase cleavage sequences
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
US6831059B2 (en) * 2000-10-20 2004-12-14 Allergan, Inc. Compositions and methods for treating gonadotrophin related illnesses
US6632638B1 (en) * 2000-11-17 2003-10-14 Amgen, Inc. Enhanced solubility of recombinant proteins using Uracil DNA glycosylase inhibitor
AU2002233230B2 (en) * 2000-12-20 2007-02-01 F. Hoffmann-La Roche Ag Erythropoietin conjugates
WO2002061064A2 (de) * 2001-02-01 2002-08-08 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin
CA2441378A1 (en) * 2001-03-22 2002-10-03 Dendreon San Diego Llc Nucleic acid molecules encoding serine protease cvsp14, the encoded polypeptides and methods based thereon

Similar Documents

Publication Publication Date Title
JP2004520049A5 (enExample)
GB2424889A (en) Gene expression technique
EP1328653A4 (en) HIGHLY EXPRESSABLE GENES
WO2002026946A3 (en) Fad4, fad5, fad5-2, and fad6, fatty acid desaturase family members and uses thereof
EP1422237A3 (en) Methods for the recombinant production of antifusogenic peptides
AU4200897A (en) Novel host cells and methods of producing proteins
WO2005087802A3 (en) Process for producing polypeptides
DK1903105T3 (da) Fremgangsmåde til fermentativ fremstilling af proteiner
AU4940096A (en) Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
AU2003238717A1 (en) POLYPEPTIDES HAVING Alpha- H-Alpha-AMINO ACID AMIDE RACEMASE ACTIVITY AND NUCLEIC ACIDS ENCODING THE SAME
DE502007005530D1 (de) Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
DK1585822T3 (da) Promotorvarianter til ekspression af gener i en fungal celle
AU2001276036A1 (en) Use of cyp52a2a promoter to increase gene expression in yeast
DE60334109D1 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
DK1905839T4 (da) Fremgangsmåde til fermentativ fremstilling af proteiner
TW200638944A (en) Recombinant enterovirus 71 neutralizing antibodies and use thereof
DE59812768D1 (de) Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
DK1546302T3 (da) Fremgangsmåder til fremstilling af pattedyrs-trypsiner
WO2010013789A1 (ja) タンパク質製造方法，融合タンパク質及び抗血清
DE60226343D1 (de) Zur glucoseregulierten produktion von menschlichem insulin in somatischen zelllinien geeignete nukleinsäurekonstrukte
CN117844665A (zh) 一种重组酵母工程菌株及其制备和在降解pet塑料中的应用
EP1310551A4 (en) VIRAL-LIKE MICROGRAINS AND METHOD FOR THE PRODUCTION THEREOF
DE60116337D1 (de) Verfahren zum screening von hochaktiven proteasen und hemmstoffen
WO2009057533A1 (ja) アミノペプチダーゼの製造方法
ATE522604T1 (de) Verfahren zur herstellung von proteinen durch elektropulsierung