DE60334109D1 - Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit - Google Patents

Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit

Info

Publication number
DE60334109D1
DE60334109D1 DE60334109T DE60334109T DE60334109D1 DE 60334109 D1 DE60334109 D1 DE 60334109D1 DE 60334109 T DE60334109 T DE 60334109T DE 60334109 T DE60334109 T DE 60334109T DE 60334109 D1 DE60334109 D1 DE 60334109D1
Authority
DE
Germany
Prior art keywords
polymorpha
foreign protein
yapsin
lack
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60334109T
Other languages
English (en)
Inventor
Hyun-Ah Kang
Sang-Ki Rhee
Min-Jeong Sohn
Jeong-Yoon Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Leadbio Inc
Bioholdings Co Ltd
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Leadbio Inc
Bioholdings Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB, Leadbio Inc, Bioholdings Co Ltd filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Application granted granted Critical
Publication of DE60334109D1 publication Critical patent/DE60334109D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60334109T 2002-06-29 2003-06-28 Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit Expired - Lifetime DE60334109D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20020037718 2002-06-29
PCT/KR2003/001279 WO2004003204A1 (en) 2002-06-29 2003-06-28 Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same

Publications (1)

Publication Number Publication Date
DE60334109D1 true DE60334109D1 (de) 2010-10-21

Family

ID=29997410

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60334109T Expired - Lifetime DE60334109D1 (de) 2002-06-29 2003-06-28 Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit

Country Status (7)

Country Link
US (1) US7262287B2 (de)
EP (1) EP1534835B1 (de)
KR (1) KR100496758B1 (de)
AT (1) ATE480624T1 (de)
AU (1) AU2003237061A1 (de)
DE (1) DE60334109D1 (de)
WO (1) WO2004003204A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134659A1 (en) * 2003-04-18 2007-06-14 Warnex Reasearch, Inc. Polynucleotides for the detection of salmonella species
KR100977446B1 (ko) * 2006-06-16 2010-08-24 한국생명공학연구원 분비 스트레스 반응을 조절하는 한세눌라 폴리모르파의신규한 유전자 및 상기 유전자를 이용하여 재조합 단백질의분비 발현 효율을 증가시키는 방법
EP2134853B1 (de) 2007-04-03 2018-07-18 Oxyrane UK Limited Glycosylierung von molekülen
US9120871B2 (en) * 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
CA2775938C (en) 2009-09-29 2021-08-10 Oxyrane Uk Limited Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
CN102834509A (zh) 2009-11-19 2012-12-19 奥克西雷恩英国有限公司 生成哺乳动物样复合n-聚糖的酵母菌株
US8815541B2 (en) 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
WO2012042386A2 (en) 2010-09-29 2012-04-05 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
EP2622089B1 (de) 2010-09-29 2023-11-01 Oxyrane UK Limited Demannosylierung von phosphorylierten n-glycanen
CA2867237C (en) 2012-03-15 2024-01-02 Wouter Vervecken Methods and materials for treatment of pompe's disease
KR102558303B1 (ko) * 2017-10-03 2023-07-21 볼트 쓰레즈, 인크. 재조합 실크 제조를 위한 변형 균주
US10647975B2 (en) 2017-10-03 2020-05-12 Bolt Threads, Inc. Modified strains for the production of recombinant silk

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68922014T2 (de) * 1988-01-27 1995-08-03 M & D Research Co Protease.
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH07108232B2 (ja) * 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
GB9404270D0 (en) 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
WO1998001535A1 (en) * 1996-07-05 1998-01-15 Novo Nordisk A/S Method for the production of polypeptides
US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
KR100246932B1 (ko) * 1997-09-12 2000-03-15 박호군 Yap3 유전자가 결손된 신규한 효모균주 및 그를 이용한 재조합 인체 부갑상선호르몬의 생산
JP2002537795A (ja) * 1999-03-04 2002-11-12 コリア リサーチ インスティテュート オブ バイオサイエンス アンド バイオテクノロジー ハンセヌラポリモルパ変異体及びそれらを利用する組換え蛋白質の製造方法
KR100441384B1 (ko) 2002-01-08 2004-07-21 주식회사 안지오랩 인간 혈청 알부민-팀프-2 융합 단백질 발현시스템 및재조합 인간 혈청 알부민-팀프-2 융합 단백질
KR102008884B1 (ko) 2014-01-23 2019-08-09 동우 화인켐 주식회사 실리콘계 화합물막 식각액 조성물
KR102001775B1 (ko) 2018-04-10 2019-07-18 조성은 Led 렌즈 및 이를 포함한 led 패키지

Also Published As

Publication number Publication date
US7262287B2 (en) 2007-08-28
ATE480624T1 (de) 2010-09-15
KR20040002806A (ko) 2004-01-07
US20050239074A1 (en) 2005-10-27
WO2004003204A1 (en) 2004-01-08
EP1534835A1 (de) 2005-06-01
KR100496758B1 (ko) 2005-06-22
EP1534835A4 (de) 2005-12-14
EP1534835B1 (de) 2010-09-08
AU2003237061A1 (en) 2004-01-19
AU2003237061A8 (en) 2004-01-19

Similar Documents

Publication Publication Date Title
ATE532870T1 (de) Verfahren zur herstellung von transglutaminase
DE69939901D1 (de) Verfahren und materialien zur herstellung von glucosamin
DE60334109D1 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
DE60227653D1 (de) Verfahren zur herstellung von l-aminosäuren, unter verwendung von stämmen aus der familie der enterohalten
DE502006008613D1 (de) Verfahren zur fermentativen Herstellung von Proteinen
DE10345772A1 (de) Verfahren zur Herstellung von 3-Methylamino-1-(thien-2-yl)-propan-1-ol
DE502004000547D1 (de) Verfahren zur fermentativen Herstellung von L-Methionin
CN107384899B (zh) 一种真菌来源的酸性蛋白酶g412及其基因和应用
ATE406447T1 (de) Neue carbonylreduktase, diese codierendes gen und verfahren zur produktion optisch-aktiver alkohole damit
US9347107B2 (en) Vector containing multiple nucleotide sequences for the expression of enzymes
US11447780B2 (en) Preparation of wheat cysteine protease triticain-alpha produced in soluble form and method of producing same
JPS60500043A (ja) 原核細胞又は真核細胞中で合成される融合蛋白質から成熟蛋白質を作る方法
DE60302776D1 (de) Verfahren zur rekombinanten Herstellung von Polypeptiden
DE60328802D1 (de) Verfahren zur herstellung von säugetier-trypsinen
EP2067857A4 (de) Verfahren zur dipeptidherstellung
DE60124921D1 (de) GENE, WELCHE FüR LUZIFERIN REGENERIERENDE PROTEINE KODIEREN, REKOMBINANTE DNA UND VERFAHREN ZUR HERSTELLUNG EINES LUZIFERIN REGENERIERENDEN PROTEINS
CN103282490B (zh) 在酵母中产生肠激酶的组合物和方法
Guerrero‐Olazarán et al. Recombinant shrimp (Litopenaeus vannamei) trypsinogen production in Pichia pastoris
DE50211058D1 (de) Verfahren zur herstellung von arachidonsäure in transgenen organismen
EP1262551A3 (de) Sorbitol-Dehydrogenase Gen, rekombinante DNA, und Verfahren zur Herstellung von Sorbitol-Dehydrogenase
JP2938352B2 (ja) 組換え人マトリライシンの製造方法
ATE465268T1 (de) Verfahren zur mikrobiologischen isomerisierung von alpha-hydroxycarbonsäuren
DE60123077D1 (de) Gene welche für proteine, die befähigt sind luziferin zu regenerieren, kodieren, rekombinante dna und verfahren zur herstellung von proteinen, die befähigt sind luziferin zu regenerieren
DE60318882D1 (de) Uroplakin-ii-promotor aus schwein und verfahren zur herstellung nützlicher proteine unter verwendung des promotors
EP1712627A4 (de) Polypeptid mit amidaseaktivität und dessen gen