DE50204952D1 - Verfahren zur herstellung von rekombinantem trypsin - Google Patents

Verfahren zur herstellung von rekombinantem trypsin

Info

Publication number
DE50204952D1
DE50204952D1 DE50204952T DE50204952T DE50204952D1 DE 50204952 D1 DE50204952 D1 DE 50204952D1 DE 50204952 T DE50204952 T DE 50204952T DE 50204952 T DE50204952 T DE 50204952T DE 50204952 D1 DE50204952 D1 DE 50204952D1
Authority
DE
Germany
Prior art keywords
trypsin
recombinant
trypsine
preparation
trypsinogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE50204952T
Other languages
German (de)
English (en)
Inventor
Rainer Mueller
Stephan Glaser
Frank Geipel
Johann-Peter Thalhofer
Bernhard Rexer
Claus Schneider
Michael Ratka
Stephanie Ronning
Hellmut Eckstein
Claudia Giessel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8176376&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE50204952(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE50204952D1 publication Critical patent/DE50204952D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE50204952T 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin Expired - Lifetime DE50204952D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01102342 2001-02-01
PCT/EP2002/001072 WO2002061064A2 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Publications (1)

Publication Number Publication Date
DE50204952D1 true DE50204952D1 (de) 2005-12-22

Family

ID=8176376

Family Applications (1)

Application Number Title Priority Date Filing Date
DE50204952T Expired - Lifetime DE50204952D1 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Country Status (10)

Country Link
US (2) US7276605B2 (enExample)
EP (1) EP1399568B1 (enExample)
JP (1) JP4159878B2 (enExample)
CN (1) CN1545553B (enExample)
AT (1) ATE310092T1 (enExample)
CA (1) CA2437342C (enExample)
CZ (1) CZ303658B6 (enExample)
DE (1) DE50204952D1 (enExample)
DK (1) DK1399568T3 (enExample)
WO (1) WO2002061064A2 (enExample)

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ES2370657T3 (es) * 2005-09-14 2011-12-21 Sanofi-Aventis Deutschland Gmbh Escisión de precursores de insulinas mediante una variante de tripsina.
AU2009336710A1 (en) * 2009-01-06 2011-06-30 Nestec S.A. Processing of macronutrients
CN101967469B (zh) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 一种高稳定性的重组胰蛋白酶的生产及应用
CN102482675B (zh) * 2009-09-10 2015-04-29 拜康有限公司 新型脂酶原-牛胰蛋白酶原融合蛋白
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN103805584B (zh) * 2012-11-12 2016-08-24 宜昌东阳光长江药业股份有限公司 一种重组胰蛋白酶纯化方法
CN103405756B (zh) * 2013-07-10 2014-12-24 浙江众益制药股份有限公司 药物组合物复方消化酶胶囊(ii)及其制备方法
MA39835A (fr) 2014-04-17 2017-02-22 Biogen Ma Inc Compositions et procédés de modulation de l'épissage du smn2 chez un patient
CN104046605A (zh) * 2014-05-29 2014-09-17 中国科学院广州能源研究所 一种嗜温耐乙醇β-葡萄糖苷酶及其编码基因和应用
US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
JP6686361B2 (ja) * 2014-11-26 2020-04-22 東ソー株式会社 細胞回収のための標本作製方法
WO2016164896A2 (en) 2015-04-10 2016-10-13 Ionis Pharmaceuticals, Inc. Modulation of smn expression
WO2017218884A1 (en) 2016-06-16 2017-12-21 Ionis Pharmaceuticals, Inc. Combinations for the modulation of smn expression
CN106350497B (zh) * 2016-11-24 2019-07-16 天津大学 一种胰蛋白酶及其制备方法与应用
CN110511916B (zh) * 2019-08-06 2021-06-01 杭州浦泰生物科技有限公司 一种重组胰蛋白酶的生产工艺
AR121446A1 (es) 2020-02-28 2022-06-08 Ionis Pharmaceuticals Inc Compuestos y métodos para modular smn2
CN115216463B (zh) * 2022-06-15 2023-08-15 武汉瀚海新酶生物科技有限公司 具有稳定性的重组胰蛋白酶及其制备方法和应用
CN120225684A (zh) 2022-11-23 2025-06-27 豪夫迈·罗氏有限公司 用于增加重组蛋白表达的方法

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CA2102673A1 (en) 1992-11-13 1994-05-14 Michael G. Greaney Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith
US5472692A (en) * 1993-07-02 1995-12-05 New England Deaconess Hospital Corporation Pro-urokinase mutants
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ATE331798T1 (de) * 1996-04-19 2006-07-15 Jackson H M Found Military Med Verfahren zur anregung einer immunantwort durch verabreichung von nutzorganismen, die intimin allein oder als fusionsprotein mit einem oder mehreren anderen antigenen exprimieren
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JP4515542B2 (ja) * 1997-02-10 2010-08-04 ジェネンテック， インコーポレイテッド ヒレグリン変異体
IL134653A0 (en) 1997-08-22 2001-04-30 Roche Diagnostics Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
US20030207402A1 (en) 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
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AU6388499A (en) * 1998-09-21 2000-04-10 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
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Also Published As

Publication number Publication date
WO2002061064A3 (de) 2003-12-24
CZ303658B6 (cs) 2013-02-06
CN1545553B (zh) 2011-09-21
US20040203095A1 (en) 2004-10-14
EP1399568A2 (de) 2004-03-24
JP4159878B2 (ja) 2008-10-01
DK1399568T3 (da) 2006-03-27
JP2004520049A (ja) 2004-07-08
CZ20032337A3 (cs) 2004-04-14
CA2437342A1 (en) 2002-08-08
ATE310092T1 (de) 2005-12-15
US7666629B2 (en) 2010-02-23
EP1399568B1 (de) 2005-11-16
CA2437342C (en) 2013-08-06
US20080064084A1 (en) 2008-03-13
CN1545553A (zh) 2004-11-10
US7276605B2 (en) 2007-10-02
WO2002061064A2 (de) 2002-08-08

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