DE60040771D1 - Verfahren zur kontrolle der spaltung mit ompt protease - Google Patents

Verfahren zur kontrolle der spaltung mit ompt protease

Info

Publication number
DE60040771D1
DE60040771D1 DE60040771T DE60040771T DE60040771D1 DE 60040771 D1 DE60040771 D1 DE 60040771D1 DE 60040771 T DE60040771 T DE 60040771T DE 60040771 T DE60040771 T DE 60040771T DE 60040771 D1 DE60040771 D1 DE 60040771D1
Authority
DE
Germany
Prior art keywords
amino acid
polypeptide
ompt protease
site
amino acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60040771T
Other languages
English (en)
Inventor
Kazuaki Okuno
Masayuki Yabuta
Kazuhiro Ohsuye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asubio Pharma Co Ltd
Original Assignee
Asubio Pharma Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asubio Pharma Co Ltd filed Critical Asubio Pharma Co Ltd
Application granted granted Critical
Publication of DE60040771D1 publication Critical patent/DE60040771D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cardiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60040771T 1999-03-04 2000-03-03 Verfahren zur kontrolle der spaltung mit ompt protease Expired - Lifetime DE60040771D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP5773199 1999-03-04
PCT/JP2000/001309 WO2000052193A1 (fr) 1999-03-04 2000-03-03 Procede de controle de clivage par la protease de l'ompt

Publications (1)

Publication Number Publication Date
DE60040771D1 true DE60040771D1 (de) 2008-12-24

Family

ID=13064080

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60040771T Expired - Lifetime DE60040771D1 (de) 1999-03-04 2000-03-03 Verfahren zur kontrolle der spaltung mit ompt protease

Country Status (12)

Country Link
US (1) US7344856B1 (de)
EP (1) EP1076097B1 (de)
JP (1) JP4471501B2 (de)
KR (1) KR100916169B1 (de)
CN (1) CN100445394C (de)
AT (1) ATE414164T1 (de)
AU (1) AU758633B2 (de)
CA (1) CA2327524C (de)
DE (1) DE60040771D1 (de)
ES (1) ES2316352T3 (de)
IL (1) IL139338A0 (de)
WO (1) WO2000052193A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2472235C (en) * 2002-04-11 2012-05-22 Daiichi Suntory Pharma Co., Ltd. A method for producing a modified peptide
RU2395582C2 (ru) * 2003-09-30 2010-07-27 Асубио Фарма Ко., Лтд. СПОСОБ РАСЩЕПЛЕНИЯ ПОЛИПЕПТИДОВ С ИСПОЛЬЗОВАНИЕМ ВАРИАНТА ПРОТЕАЗЫ ОmpТ
US8586294B2 (en) 2004-05-18 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Detection of protein translocation by beta-galactosidase reporter fragment complementation
US8030447B2 (en) 2006-09-20 2011-10-04 The Board Of Regents Of The University Of Texas System Substrate peptide sequences for plague plasminogen activator and uses thereof
CN102707054B (zh) * 2012-06-18 2014-11-05 黑龙江八一农垦大学 大肠埃希氏菌OmpT抗体间接ELISA检测方法
US9650621B2 (en) 2012-10-12 2017-05-16 Sutro Biopharma, Inc. Proteolytic inactivation of select proteins in bacterial extracts for improved expression
US20220249369A1 (en) * 2018-09-19 2022-08-11 Georgia Tech Research Corporation Biological bits as computable elements within living systems

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
WO1998049286A2 (en) 1997-05-01 1998-11-05 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
CA2284847A1 (en) 1998-01-30 1999-08-05 Suntory Limited Process for producing peptides using a helper peptide

Also Published As

Publication number Publication date
ATE414164T1 (de) 2008-11-15
EP1076097A1 (de) 2001-02-14
EP1076097B1 (de) 2008-11-12
CA2327524C (en) 2010-02-02
US7344856B1 (en) 2008-03-18
CN1302337A (zh) 2001-07-04
KR100916169B1 (ko) 2009-09-08
CN100445394C (zh) 2008-12-24
WO2000052193A1 (fr) 2000-09-08
ES2316352T3 (es) 2009-04-16
IL139338A0 (en) 2001-11-25
AU758633B2 (en) 2003-03-27
JP4471501B2 (ja) 2010-06-02
EP1076097A4 (de) 2003-02-05
CA2327524A1 (en) 2000-09-08
KR20010043333A (ko) 2001-05-25
AU2828800A (en) 2000-09-21

Similar Documents

Publication Publication Date Title
ATE124456T1 (de) Verfahren zur enzymatischen spaltung rekombinanter proteine unter verwendung von iga- proteasen.
DE3586773D1 (de) Verfahren zur beseitigung von n-endaminosaeureresten aus eukaryotpolypeptidanlagen und so erzeugte polypeptide.
DE69432744D1 (de) Verbessertes verfahren zur rückfaltung der proteine
HUT54213A (en) Process for the enzymatic removal of n-terminal sequence of proteins
WO2001029242A3 (en) Post-translational modification of recombinant proteins produced in plants
DE60320362D1 (de) Verfahren zur Herstellung von Dipeptiden
ATE346095T1 (de) Glycoprotein vi - fc fusions protein zur behandlung von gefässerkrankungen
DE50204952D1 (de) Verfahren zur herstellung von rekombinantem trypsin
DE60040771D1 (de) Verfahren zur kontrolle der spaltung mit ompt protease
ATE341640T1 (de) Verfahren zur herstellung von nicht-proteinogenen l-aminosäuren
DE69739013D1 (de) Verfahren zur Herstellung sekretorischer KEX2-Derivate
ATE253639T1 (de) Verfahren zur herstellung von peptiden
NO930428L (no) Proteinstrukturen til plantetoksingelonin
DK1095141T3 (da) Fremgangsmåde til fremstilling af et peptid med pI-værdi over 8 eller under 5
DE69732583D1 (de) Verfahren zur Spaltung eines chimeren Proteins mittels eines 'processing'-Enzyms
DE60037508D1 (de) Verfahren zur herstellung von rekombinantem insulin durch fusionsproteine
DE60038281D1 (de) Verfahren zur herstellung von optisch aktiven pyridinethanol-derivaten
ATE144551T1 (de) Methode zur produktion von glukagon
DE60327228D1 (de) Verfahren zur herstellung von wachstumshormon und antagonisten davon mit geringeren konzentrationen an isoformen verunreinigungen davon
DE60214361D1 (de) Verfahren zur herstellung von gallensäure
ATE224403T1 (de) Verwendung von kaseinfragmenten als wachstumerförderer
DE50313042D1 (de) Nukleotidsequenzen codierend für deregulierte phosphoglycerat-dehydrogenasen coryneformer bakterien sowie verfahren zur herstellung von l-serin

Legal Events

Date Code Title Description
8364 No opposition during term of opposition