DE69739013D1 - Verfahren zur Herstellung sekretorischer KEX2-Derivate - Google Patents

Verfahren zur Herstellung sekretorischer KEX2-Derivate

Info

Publication number
DE69739013D1
DE69739013D1 DE69739013T DE69739013T DE69739013D1 DE 69739013 D1 DE69739013 D1 DE 69739013D1 DE 69739013 T DE69739013 T DE 69739013T DE 69739013 T DE69739013 T DE 69739013T DE 69739013 D1 DE69739013 D1 DE 69739013D1
Authority
DE
Germany
Prior art keywords
derivatives
kex2 protease
acid sequence
amino acid
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739013T
Other languages
English (en)
Inventor
Koji Magota
Toyofumi Masuda
Yuji Suzuki
Masayuki Yabuta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daiichi Sankyo Co Ltd
Original Assignee
Asubio Pharma Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asubio Pharma Co Ltd filed Critical Asubio Pharma Co Ltd
Application granted granted Critical
Publication of DE69739013D1 publication Critical patent/DE69739013D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE69739013T 1996-03-04 1997-03-04 Verfahren zur Herstellung sekretorischer KEX2-Derivate Expired - Lifetime DE69739013D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP7321796 1996-03-04
JP35258096 1996-12-16

Publications (1)

Publication Number Publication Date
DE69739013D1 true DE69739013D1 (de) 2008-11-13

Family

ID=26414373

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69739013T Expired - Lifetime DE69739013D1 (de) 1996-03-04 1997-03-04 Verfahren zur Herstellung sekretorischer KEX2-Derivate

Country Status (10)

Country Link
US (1) US5885821A (de)
EP (1) EP0794254B1 (de)
KR (1) KR100548653B1 (de)
CN (1) CN1216988C (de)
AT (1) ATE409748T1 (de)
AU (1) AU731201B2 (de)
CA (1) CA2198968C (de)
DE (1) DE69739013D1 (de)
ES (1) ES2312171T3 (de)
TW (1) TW557300B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
US6517974B1 (en) * 1998-01-30 2003-02-11 Canon Kabushiki Kaisha Lithium secondary battery and method of manufacturing the lithium secondary battery
SK782002A3 (en) 1999-07-21 2003-08-05 Lexigen Pharm Corp FC fusion proteins for enhancing the immunogenicity of protein and peptide antigens
EP1493747B1 (de) 2002-04-11 2012-12-19 Daiichi Sankyo Company, Limited Verfahren zur herstellung von modifiziertem peptid
WO2004024862A2 (en) * 2002-09-13 2004-03-25 Wockhardt Limited Yeast protein expression secretion system
ES2558102T3 (es) * 2003-05-06 2016-02-02 Biogen Hemophilia Inc. Proteínas quiméricas del factor de coagulación para el tratamiento de un trastorno hemostático
US20050037947A1 (en) * 2003-05-06 2005-02-17 Bitonti Alan J. Inhibition of drug binding to serum albumin
US7348004B2 (en) 2003-05-06 2008-03-25 Syntonix Pharmaceuticals, Inc. Immunoglobulin chimeric monomer-dimer hybrids
TWI353991B (en) 2003-05-06 2011-12-11 Syntonix Pharmaceuticals Inc Immunoglobulin chimeric monomer-dimer hybrids
EP2831262B1 (de) 2012-03-29 2018-02-14 Biocon Limited Sekretion von funktionellem insulinglargin direkt in das kulturmedium durch intrazelluläre überexpression von kex2p
RU2663587C2 (ru) 2013-03-06 2018-08-07 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Клетки-хозяева и способы использования
EP2968498A4 (de) 2013-03-15 2016-09-07 Biogen Ma Inc Faktor-ix-polypeptidformulierungen
CN106337042B (zh) * 2015-07-16 2019-08-09 上海雅心生物技术有限公司 双碱基酶kex2变体及其高效表达方法
CN110982808A (zh) * 2019-12-27 2020-04-10 万新医药科技(苏州)有限公司 Kex2酶的变体及稳定表达的方法
CN114717282A (zh) * 2021-12-31 2022-07-08 北京盛拓达生物技术有限公司 一种组合物及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
EP0558024B1 (de) * 1992-02-28 1996-08-14 Suntory Limited Neuer Vektor, einen von Methanol und/oder Glyzerin induzierbaren Promotor enthaltend

Also Published As

Publication number Publication date
CN1168922A (zh) 1997-12-31
KR100548653B1 (ko) 2006-07-14
EP0794254A3 (de) 2000-01-05
AU731201B2 (en) 2001-03-29
ES2312171T3 (es) 2009-02-16
CA2198968A1 (en) 1997-09-04
EP0794254A2 (de) 1997-09-10
CN1216988C (zh) 2005-08-31
EP0794254B1 (de) 2008-10-01
AU1503797A (en) 1997-09-11
US5885821A (en) 1999-03-23
ATE409748T1 (de) 2008-10-15
TW557300B (en) 2003-10-11
CA2198968C (en) 2010-02-09
KR970065558A (ko) 1997-10-13

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: DAIICHI SANKYO COMPANY, LIMITED, TOKIO, JP