WO2001029242A3 - Post-translational modification of recombinant proteins produced in plants - Google Patents

Post-translational modification of recombinant proteins produced in plants Download PDF

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Publication number
WO2001029242A3
WO2001029242A3 PCT/US2000/029027 US0029027W WO0129242A3 WO 2001029242 A3 WO2001029242 A3 WO 2001029242A3 US 0029027 W US0029027 W US 0029027W WO 0129242 A3 WO0129242 A3 WO 0129242A3
Authority
WO
WIPO (PCT)
Prior art keywords
post
translational modification
plants
recombinant proteins
proteins produced
Prior art date
Application number
PCT/US2000/029027
Other languages
French (fr)
Other versions
WO2001029242A2 (en
Inventor
Douglas Russell
Siva Manjunath
Ronald Bassuner
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Priority to AU15736/01A priority Critical patent/AU1573601A/en
Priority to EP00978257A priority patent/EP1224309A2/en
Priority to CA002388432A priority patent/CA2388432A1/en
Priority to BR0014945-4A priority patent/BR0014945A/en
Publication of WO2001029242A2 publication Critical patent/WO2001029242A2/en
Publication of WO2001029242A3 publication Critical patent/WO2001029242A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

Abstract

The present invention is directed to methods for producing a post-translationally modified heterologous polypeptide in a plant host system by altering the natural post-translational modification abilities of that plant host system. In another aspect of this method, altering the natural post-translational modifications is done by transforming the plant host system with one or more nucleic acid sequences encoding a post-translational modification enzyme.
PCT/US2000/029027 1999-10-21 2000-10-20 Post-translational modification of recombinant proteins produced in plants WO2001029242A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU15736/01A AU1573601A (en) 1999-10-21 2000-10-20 Post-translational modification of recombinant proteins produced in plants
EP00978257A EP1224309A2 (en) 1999-10-21 2000-10-20 Post-translational modification of recombinant proteins produced in plants
CA002388432A CA2388432A1 (en) 1999-10-21 2000-10-20 Post-translational modification of recombinant proteins produced in plants
BR0014945-4A BR0014945A (en) 1999-10-21 2000-10-20 Posttranslational modification of recombinant proteins produced in plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16075899P 1999-10-21 1999-10-21
US60/160,758 1999-10-21
US19528200P 2000-04-07 2000-04-07
US60/195,282 2000-04-07

Publications (2)

Publication Number Publication Date
WO2001029242A2 WO2001029242A2 (en) 2001-04-26
WO2001029242A3 true WO2001029242A3 (en) 2002-02-21

Family

ID=26857200

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/029027 WO2001029242A2 (en) 1999-10-21 2000-10-20 Post-translational modification of recombinant proteins produced in plants

Country Status (5)

Country Link
EP (1) EP1224309A2 (en)
AU (1) AU1573601A (en)
BR (1) BR0014945A (en)
CA (1) CA2388432A1 (en)
WO (1) WO2001029242A2 (en)

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CN100427599C (en) * 2003-12-26 2008-10-22 吉林大学第一医院 ALBSIN/IL-18MAT hybridization cytokine

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US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
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CA2450019A1 (en) * 2001-06-08 2002-12-19 Icon Genetics, Inc. Production of proteins in plants
EP1463751B1 (en) 2001-12-21 2013-05-22 Human Genome Sciences, Inc. Albumin fusion proteins
KR101228276B1 (en) 2002-03-19 2013-01-30 스티칭 디엔스트 랜드보위쿤디그 온데조에크 Optimizing Glycan Processing in Plants
US7897842B2 (en) 2002-03-19 2011-03-01 Plant Research International B.V. GnTIII expression in plants
AU2008202355B2 (en) * 2002-03-19 2011-04-07 Stichting Dienst Landbouwkundig Onderzoek Optimizing glycan processing in plants
AR042145A1 (en) 2002-11-27 2005-06-08 Dow Agrociences Llc IMMUNOGLOBULIN PRODUCTION IN PLANTS WITH A REDUCED FUCOCILATION
JP4559358B2 (en) * 2002-12-20 2010-10-06 グリーンオベーション バイオテク ゲーエムベーハー Production of heterologous glycosylated proteins in moss cells
ATE335084T1 (en) * 2002-12-20 2006-08-15 Greenovation Biotech Gmbh METHOD FOR PRODUCING GLYCOSYLATED PROTEINS IN BRYOPHYTE CELLS
AU2003298984A1 (en) * 2002-12-27 2004-08-23 Shenzhen Tsinghua Yuanxing Bio-Pharm Science And Technology Co., Ltd. Method of preparing a vaccine and anti-tumor vaccines
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US8455717B2 (en) 2004-09-29 2013-06-04 Collplant Ltd. Collagen producing plants and methods of generating and using same
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BRPI0707290A2 (en) 2006-01-17 2011-08-16 Biolex Therapeutics Inc compositions and methods for humanization and optimization of n-glycans in plants
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CN101622354A (en) * 2006-11-08 2010-01-06 国家科学研究中心 A set of sequences for targeting expression and control of the post-translationnal modifications of a recombinant polypeptide
MX341988B (en) 2007-04-17 2016-09-08 Stichting Dienst Landbouwkunding Onderzoek Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases.
HUE031698T2 (en) * 2007-06-15 2017-07-28 Medicago Inc Modifying glycoprotein production in plants
WO2009056155A1 (en) * 2007-10-31 2009-05-07 Bayer Bioscience N.V. Method to produce modified plants with altered n-glycosylation pattern
MX2010004910A (en) 2007-11-08 2010-05-14 Neogenix Oncology Inc Recombinant monoclonal antibodies and corresponding antigens for colon and pancreatic cancers.
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US20140302999A1 (en) * 2011-11-03 2014-10-09 Magdalena Leszczyniecka Method for determination of pharmacological properties of recombinant proteins
WO2014130922A1 (en) 2013-02-25 2014-08-28 Trustees Of Boston University Compositions and methods for treating fungal infections
GB201308120D0 (en) * 2013-05-06 2013-06-12 Baden Wuerttemberg Stiftung Gmbh Recombinant protein and method for its production
JP6450381B2 (en) 2013-07-05 2019-01-09 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャリゼーション Soluble MIC neutralizing monoclonal antibody for treating cancer
JPWO2015050005A1 (en) * 2013-10-02 2017-03-09 株式会社ＵｎｉＢｉｏ Protein composition for promoting cell proliferation via EGF receptor
EP3221455B1 (en) 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
EP3442577A4 (en) * 2016-04-15 2020-03-25 REGENXBIO Inc. Treatment of ocular diseases with fully-human post-translationally modified anti-vegf fab
WO2017180936A1 (en) 2016-04-15 2017-10-19 The Trustees Of The University Of Pennsylvania Compositions for treatment of wet age-related macular degeneration
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JP2024505600A (en) 2021-02-03 2024-02-06 モーツァルト セラピューティクス， インコーポレイテッド Binders and how to use them
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WO2022226317A1 (en) 2021-04-23 2022-10-27 Profoundbio Us Co. Anti-cd70 antibodies, conjugates thereof and methods of using the same
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100427599C (en) * 2003-12-26 2008-10-22 吉林大学第一医院 ALBSIN/IL-18MAT hybridization cytokine

Also Published As

Publication number Publication date
EP1224309A2 (en) 2002-07-24
CA2388432A1 (en) 2001-04-26
WO2001029242A2 (en) 2001-04-26
AU1573601A (en) 2001-04-30
BR0014945A (en) 2004-08-31

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