AU2001276036A1 - Use of cyp52a2a promoter to increase gene expression in yeast - Google Patents

Use of cyp52a2a promoter to increase gene expression in yeast

Info

Publication number
AU2001276036A1
AU2001276036A1 AU2001276036A AU7603601A AU2001276036A1 AU 2001276036 A1 AU2001276036 A1 AU 2001276036A1 AU 2001276036 A AU2001276036 A AU 2001276036A AU 7603601 A AU7603601 A AU 7603601A AU 2001276036 A1 AU2001276036 A1 AU 2001276036A1
Authority
AU
Australia
Prior art keywords
nucleic acid
yeast
gene expression
increase gene
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001276036A
Inventor
David L. Craft
Dudley Eirich
C. Ron Wilson
Yeyan Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cognis Corp
Original Assignee
Cognis Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cognis Corp filed Critical Cognis Corp
Publication of AU2001276036A1 publication Critical patent/AU2001276036A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.
AU2001276036A 2000-07-26 2001-07-25 Use of cyp52a2a promoter to increase gene expression in yeast Abandoned AU2001276036A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22085000P 2000-07-26 2000-07-26
US60220850 2000-07-26
US09/911,781 US6673613B2 (en) 2000-07-26 2001-07-24 Use of CYP52A2A promoter to increase gene expression in yeast
US09911781 2001-07-24
PCT/US2001/023377 WO2002008412A2 (en) 2000-07-26 2001-07-25 Use of cyp52a2a promoter to increase gene expression in yeast

Publications (1)

Publication Number Publication Date
AU2001276036A1 true AU2001276036A1 (en) 2002-02-05

Family

ID=26915242

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001276036A Abandoned AU2001276036A1 (en) 2000-07-26 2001-07-25 Use of cyp52a2a promoter to increase gene expression in yeast

Country Status (8)

Country Link
US (2) US6673613B2 (en)
EP (1) EP1303593B1 (en)
CN (1) CN1444649A (en)
AT (1) ATE383420T1 (en)
AU (1) AU2001276036A1 (en)
DE (1) DE60132332T2 (en)
DK (1) DK1303593T3 (en)
WO (1) WO2002008412A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002344369A1 (en) 2001-06-01 2002-12-16 Epidauros Biotechnologie Ag Polymorphisms in the human gene for cytochrome p450 polypeptide 2c8 and their use in diagnostic and therapeutic applications
AU2003247411A1 (en) * 2002-05-23 2003-12-12 Cognis Corporation NON-REVERTIBLE Beta-OXIDATION BLOCKED CANDIDA TROPICALIS
US7388084B2 (en) * 2002-08-16 2008-06-17 Cognis Corporation Promoter motifs in Candida tropicalis
FR2879215B1 (en) * 2004-12-15 2010-08-20 Inst Francais Du Petrole PRODUCTION OF DICARBOXYLIC ACIDS BY ENHANCED MUTANT STRAINS OF YARROWIA LIPOLYTICA
ES2585239T3 (en) * 2007-08-01 2016-10-04 Idera Pharmaceuticals, Inc. New synthetic agonists of TLR9
CA2729626C (en) * 2008-07-11 2017-12-12 Jean-Marc Nicaud New mutant yeast strains capable of accumulating a large quantity of lipids
SG176970A1 (en) 2009-07-02 2012-02-28 Verdezyne Inc Biological methods for preparing adipic acid
WO2012071439A1 (en) * 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds
CN102212546B (en) * 2011-02-28 2013-04-17 福建福大百特科技发展有限公司 Integrative Candida maltose gene expression system and applications thereof
WO2012138613A1 (en) * 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
CA2841794C (en) * 2011-07-06 2019-08-13 Verdezyne, Inc. Genetically modified yeast with increased alcohol dehydrogenase activity for preparing a fatty dicarboxylic acid
CN103992959B (en) * 2014-04-28 2017-02-08 中国科学院微生物研究所 Long-chain dibasic acid producing strain and preparation method and application thereof
CN110684783B (en) * 2018-07-06 2023-08-08 上海凯赛生物技术股份有限公司 Long-chain dibasic acid with low content of fatty acid impurities and production method thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254466A (en) 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
GB9703406D0 (en) * 1997-02-19 1997-04-09 Chiron Spa Expression of heterologous proteins
US6331420B1 (en) 1998-05-01 2001-12-18 C. Ron Wilson Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto
CA2349868C (en) 1998-10-05 2010-07-20 Cognis Corporation Cytochrome p450 monooxygenase and nadph cytochrome p450 oxidoreductase genes and proteins related to the omega hydroxylase complex of candida tropicalis and methods relating thereto

Also Published As

Publication number Publication date
WO2002008412A2 (en) 2002-01-31
WO2002008412A3 (en) 2002-05-30
EP1303593A2 (en) 2003-04-23
DE60132332T2 (en) 2009-01-02
US20020034788A1 (en) 2002-03-21
US20030175896A1 (en) 2003-09-18
ATE383420T1 (en) 2008-01-15
US6673613B2 (en) 2004-01-06
DK1303593T3 (en) 2008-05-19
EP1303593B1 (en) 2008-01-09
US6790640B2 (en) 2004-09-14
DE60132332D1 (en) 2008-02-21
CN1444649A (en) 2003-09-24

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