JP2004517643A5 - - Google Patents
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- Publication number
- JP2004517643A5 JP2004517643A5 JP2002565075A JP2002565075A JP2004517643A5 JP 2004517643 A5 JP2004517643 A5 JP 2004517643A5 JP 2002565075 A JP2002565075 A JP 2002565075A JP 2002565075 A JP2002565075 A JP 2002565075A JP 2004517643 A5 JP2004517643 A5 JP 2004517643A5
- Authority
- JP
- Japan
- Prior art keywords
- proteinase
- host cell
- protein
- encoding
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108010067770 Endopeptidase K Proteins 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 7
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 239000013598 vector Substances 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 3
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 239000002243 precursor Substances 0.000 claims 3
- 102000010911 Enzyme Precursors Human genes 0.000 claims 2
- 108010062466 Enzyme Precursors Proteins 0.000 claims 2
- 230000028327 secretion Effects 0.000 claims 2
- 238000011144 upstream manufacturing Methods 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 1
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000014726 immortalization of host cell Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10105911A DE10105911A1 (de) | 2001-02-09 | 2001-02-09 | Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe |
| PCT/EP2002/001144 WO2002064760A2 (de) | 2001-02-09 | 2002-02-05 | Expression der rekombinanten proteinase k aus tritirachium album in hefe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004517643A JP2004517643A (ja) | 2004-06-17 |
| JP2004517643A5 true JP2004517643A5 (enExample) | 2005-05-26 |
Family
ID=7673416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002565075A Pending JP2004517643A (ja) | 2001-02-09 | 2002-02-05 | 酵母におけるＴｒｉｔｉｒａｃｈｉｕｍａｌｂｕｍ由来の組換えプロテイナーゼＫの発現 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US7368274B2 (enExample) |
| EP (1) | EP1360283B1 (enExample) |
| JP (1) | JP2004517643A (enExample) |
| CN (1) | CN100549169C (enExample) |
| AT (1) | ATE353960T1 (enExample) |
| CA (1) | CA2435606C (enExample) |
| CZ (1) | CZ304969B6 (enExample) |
| DE (2) | DE10105911A1 (enExample) |
| DK (1) | DK1360283T3 (enExample) |
| ES (1) | ES2280516T3 (enExample) |
| WO (1) | WO2002064760A2 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7153666B2 (en) | 2003-07-17 | 2006-12-26 | General Atomics | Methods and compositions for determination of glycated proteins |
| JP2007530061A (ja) * | 2004-04-02 | 2007-11-01 | エフ．ホフマン−ラ ロシュ アーゲー | 高度に精製されたプロテナーゼｋ |
| ES2404059T3 (es) | 2006-07-25 | 2013-05-23 | General Atomics | Procedimientos para analizar el porcentaje de hemoglobina glicada |
| US7943385B2 (en) | 2006-07-25 | 2011-05-17 | General Atomics | Methods for assaying percentage of glycated hemoglobin |
| US8673646B2 (en) * | 2008-05-13 | 2014-03-18 | General Atomics | Electrochemical biosensor for direct determination of percentage of glycated hemoglobin |
| CN102839165B (zh) * | 2012-09-26 | 2014-12-10 | 金普诺安生物科技（苏州）有限公司 | 基因突变型重组蛋白酶k及其工业化生产方法 |
| CN104059900A (zh) * | 2013-03-19 | 2014-09-24 | 中国科学院天津工业生物技术研究所 | 一种蛋白酶k及其应用 |
| CN105087529A (zh) * | 2014-05-07 | 2015-11-25 | 天津旭晨科技有限公司 | 基因工程改造蛋白酶k及其生产方法 |
| CN105193640B (zh) * | 2014-06-24 | 2018-10-12 | 金普诺安蛋白质工程技术（北京）有限公司 | 蛋白酶k在皮肤保健和化妆品领域中的应用 |
| CN108450952A (zh) * | 2018-02-08 | 2018-08-28 | 深圳市唯姿贸易有限公司 | 一种细胞修复口服液 |
| CN110106160B (zh) * | 2019-05-29 | 2021-07-20 | 中国农业科学院北京畜牧兽医研究所 | 催化活性提高的碱性蛋白酶突变体prok-g及其编码基因和应用 |
| CN112048518B (zh) * | 2020-08-22 | 2022-06-24 | 江西农业大学 | 一种玉米醇溶蛋白降解酶的制备方法及其应用 |
| CN114181925B (zh) * | 2020-09-14 | 2025-01-17 | 武汉禾元生物科技股份有限公司 | 一种重组蛋白酶k工业化纯化及冻干方法 |
| CN112592931A (zh) * | 2020-12-31 | 2021-04-02 | 安徽丰原发酵技术工程研究有限公司 | 一种生产重组蛋白酶k的方法 |
| CN113337492B (zh) * | 2021-06-02 | 2023-04-07 | 武汉瀚海新酶生物科技有限公司 | 一种蛋白酶k耐热突变体 |
| CN113481225A (zh) * | 2021-07-23 | 2021-10-08 | 武汉瀚海新酶生物科技有限公司 | 一种蛋白酶k高表达工程菌株的构建及应用 |
| CN115011583A (zh) * | 2021-11-26 | 2022-09-06 | 河南合智医药科技有限公司 | 高表达高比活蛋白酶k突变体序列、毕赤酵母表达质粒的构建及菌株筛选和纯化方法 |
| PL244825B1 (pl) | 2021-11-26 | 2024-03-11 | Blirt Spolka Akcyjna | Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K |
| CN115851680A (zh) * | 2022-11-22 | 2023-03-28 | 山东恒鲁生物科技有限公司 | 一种菌的培养物及其应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3537708A1 (de) | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| AU617996B2 (en) * | 1987-04-03 | 1991-12-12 | Amgen, Inc. | Novel proteolytic enzymes |
| US5191063A (en) | 1989-05-02 | 1993-03-02 | University Of Medicine And Dentistry Of New Jersey | Production of biologically active polypeptides by treatment with an exogenous peptide sequence |
| IL117350A0 (en) | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
-
2001
- 2001-02-09 DE DE10105911A patent/DE10105911A1/de not_active Withdrawn
-
2002
- 2002-02-05 AT AT02712891T patent/ATE353960T1/de active
- 2002-02-05 US US10/468,252 patent/US7368274B2/en not_active Expired - Lifetime
- 2002-02-05 DK DK02712891T patent/DK1360283T3/da active
- 2002-02-05 CN CNB028047745A patent/CN100549169C/zh not_active Expired - Lifetime
- 2002-02-05 CZ CZ2003-2163A patent/CZ304969B6/cs not_active IP Right Cessation
- 2002-02-05 JP JP2002565075A patent/JP2004517643A/ja active Pending
- 2002-02-05 EP EP02712891A patent/EP1360283B1/de not_active Expired - Lifetime
- 2002-02-05 ES ES02712891T patent/ES2280516T3/es not_active Expired - Lifetime
- 2002-02-05 CA CA2435606A patent/CA2435606C/en not_active Expired - Lifetime
- 2002-02-05 DE DE50209478T patent/DE50209478D1/de not_active Expired - Lifetime
- 2002-02-05 WO PCT/EP2002/001144 patent/WO2002064760A2/de not_active Ceased
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