ES8604305A1 - Un metodo de hidrolizar celulosa - Google Patents
Un metodo de hidrolizar celulosaInfo
- Publication number
- ES8604305A1 ES8604305A1 ES543767A ES543767A ES8604305A1 ES 8604305 A1 ES8604305 A1 ES 8604305A1 ES 543767 A ES543767 A ES 543767A ES 543767 A ES543767 A ES 543767A ES 8604305 A1 ES8604305 A1 ES 8604305A1
- Authority
- ES
- Spain
- Prior art keywords
- recombinant fungal
- fungal cellobiohydrolases
- cellobiohydrolases
- glycosylated
- ligated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/91—Fusion polypeptide containing a motif for post-translational modification containing a motif for glycosylation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
METODO PARA LA HIDROLISIS DE CELULOSA. CONSISTE EN LA TRANSFORMACION DE UNA CELULA HOSPEDADORA RECOMBINANTE, CON UN VECTOR EFICAZ EN EXPRESAR UNA SECUENCIA DE ADN QUE CODIFICA CELULOSA FUNGICA EN UNA CELDA HOSPEDANTE RECOMBINANTE, VECTOR QUE COMPRENDE DICHA SECUENCIA DE ADN EN UNION OPERABLE CON SECUENCIAS DE CONTROL COMPATIBLES CON LA CELULA HOSPEDASDORA CITADA; CRECIMIENTO DEL HOSPEDADOR EN UN MEDIO DE CULTIVO ADECUADO; Y TRATAMIENTO DE CELULOSA CON LA CELULASA PRODUCIDA POR LAS CELULAS HOSPEDADORES TRANSFORMADAS. TIENE APLICACIONES PARA LA PRODUCCION DE GLUCOSA EMPLEADA COMO ALIMENTO O COMO INTERMEDIO PARA LA FABRICACION DE OTROS PRODUCTOS, A PARTIR DE SOLIDOS MUNICIPALES, PAPEL USADO Y SUBPRODUCTOS AGRICOLAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52821683A | 1983-08-31 | 1983-08-31 | |
US63097484A | 1984-07-16 | 1984-07-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8604305A1 true ES8604305A1 (es) | 1986-01-16 |
ES543767A0 ES543767A0 (es) | 1986-01-16 |
Family
ID=27062651
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES535511A Expired ES8602139A1 (es) | 1983-08-31 | 1984-08-30 | Un metodo para producir una proteina heterologa madura. |
ES543766A Expired ES8604304A1 (es) | 1983-08-31 | 1985-05-31 | Un metodo de hidrolizar celulosa |
ES543767A Expired ES8604305A1 (es) | 1983-08-31 | 1985-05-31 | Un metodo de hidrolizar celulosa |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES535511A Expired ES8602139A1 (es) | 1983-08-31 | 1984-08-30 | Un metodo para producir una proteina heterologa madura. |
ES543766A Expired ES8604304A1 (es) | 1983-08-31 | 1985-05-31 | Un metodo de hidrolizar celulosa |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0137280B1 (es) |
JP (2) | JPH0616709B2 (es) |
AU (1) | AU589112B2 (es) |
BR (1) | BR8404346A (es) |
CA (1) | CA1338400C (es) |
DE (2) | DE3485558D1 (es) |
DK (1) | DK419584A (es) |
ES (3) | ES8602139A1 (es) |
Families Citing this family (107)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI841500A0 (fi) * | 1984-04-13 | 1984-04-13 | Valtion Teknillinen | Foerfarande foer uppbygnande av cellulolytiska jaeststammar. |
US5320960A (en) * | 1992-04-03 | 1994-06-14 | Genencor International, Inc. | Method of preparing solution enriched in xylanase using low molecular weight alcohol, organic salt and inorganic salt |
US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
US5989870A (en) * | 1986-04-30 | 1999-11-23 | Rohm Enzyme Finland Oy | Method for cloning active promoters |
GB8610600D0 (en) * | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
GB8621626D0 (en) * | 1986-09-08 | 1986-10-15 | Health Lab Service Board | Phenylalanine ammonia lyase |
JP2507345B2 (ja) * | 1986-09-16 | 1996-06-12 | 三井東圧化学株式会社 | L―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子 |
US5610034A (en) * | 1987-04-29 | 1997-03-11 | Alko Group Ltd. | Immunoglobulin production by trichoderma |
US5688290A (en) * | 1989-10-19 | 1997-11-18 | Genencor International, Inc. | Degradation resistant detergent compositions based on cellulase enzymes |
CA2069962A1 (en) * | 1990-08-17 | 1992-02-18 | Shi-Hsiang Shen | Recombinant dna production of beta-1, 3-glucanase |
US5650322A (en) * | 1990-10-05 | 1997-07-22 | Genencor International, Inc. | Methods for stonewashing fabrics using endoglucanases |
US5290474A (en) * | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
WO1992006221A1 (en) * | 1990-10-05 | 1992-04-16 | Genencor International, Inc. | Methods for treating cotton-containing fabrics with cellulase |
US5328841A (en) * | 1990-10-05 | 1994-07-12 | Genencor International, Inc. | Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol |
CA2093422C (en) * | 1990-10-05 | 2001-04-03 | DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS | |
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JPH07506404A (ja) * | 1992-05-01 | 1995-07-13 | ジェネンコア インターナショナル インコーポレーテッド | 綿含有織物をcbh iが多いセルラーゼで処理する方法 |
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GB9400623D0 (en) * | 1994-01-14 | 1994-03-09 | Univ Leeds | Exploitation of the cellulase enzyme complex of neurospora |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4275163A (en) * | 1978-11-20 | 1981-06-23 | The United States Of America As Represented By The Secretary Of The Army | Cellulase-producing microorganism |
-
1984
- 1984-08-21 CA CA 461442 patent/CA1338400C/en not_active Expired - Lifetime
- 1984-08-29 DE DE8484110305T patent/DE3485558D1/de not_active Expired - Fee Related
- 1984-08-29 DE DE1984110305 patent/DE137280T1/de active Pending
- 1984-08-29 EP EP84110305A patent/EP0137280B1/en not_active Expired - Lifetime
- 1984-08-30 BR BR8404346A patent/BR8404346A/pt unknown
- 1984-08-30 ES ES535511A patent/ES8602139A1/es not_active Expired
- 1984-08-30 AU AU32530/84A patent/AU589112B2/en not_active Ceased
- 1984-08-31 DK DK419584A patent/DK419584A/da not_active Application Discontinuation
- 1984-08-31 JP JP59180893A patent/JPH0616709B2/ja not_active Expired - Fee Related
-
1985
- 1985-05-31 ES ES543766A patent/ES8604304A1/es not_active Expired
- 1985-05-31 ES ES543767A patent/ES8604305A1/es not_active Expired
-
1993
- 1993-10-07 JP JP5251285A patent/JP2584186B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU589112B2 (en) | 1989-10-05 |
JPH0751071A (ja) | 1995-02-28 |
DK419584D0 (da) | 1984-08-31 |
ES8604304A1 (es) | 1986-01-16 |
ES543767A0 (es) | 1986-01-16 |
DK419584A (da) | 1985-03-01 |
DE137280T1 (de) | 1986-01-16 |
JP2584186B2 (ja) | 1997-02-19 |
DE3485558D1 (de) | 1992-04-16 |
JPS60149387A (ja) | 1985-08-06 |
JPH0616709B2 (ja) | 1994-03-09 |
BR8404346A (pt) | 1985-07-30 |
EP0137280A1 (en) | 1985-04-17 |
EP0137280B1 (en) | 1992-03-11 |
ES535511A0 (es) | 1985-11-16 |
CA1338400C (en) | 1996-06-18 |
AU3253084A (en) | 1985-03-07 |
ES543766A0 (es) | 1986-01-16 |
ES8602139A1 (es) | 1985-11-16 |
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