ES543767A0 - Un metodo de hidrolizar celulosa - Google Patents
Un metodo de hidrolizar celulosaInfo
- Publication number
- ES543767A0 ES543767A0 ES543767A ES543767A ES543767A0 ES 543767 A0 ES543767 A0 ES 543767A0 ES 543767 A ES543767 A ES 543767A ES 543767 A ES543767 A ES 543767A ES 543767 A0 ES543767 A0 ES 543767A0
- Authority
- ES
- Spain
- Prior art keywords
- hydrolyzing cellulose
- hydrolyzing
- cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920002678 cellulose Polymers 0.000 title 1
- 239000001913 cellulose Substances 0.000 title 1
- 230000003301 hydrolyzing effect Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/91—Fusion polypeptide containing a motif for post-translational modification containing a motif for glycosylation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52821683A | 1983-08-31 | 1983-08-31 | |
| US63097484A | 1984-07-16 | 1984-07-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8604305A1 ES8604305A1 (es) | 1986-01-16 |
| ES543767A0 true ES543767A0 (es) | 1986-01-16 |
Family
ID=27062651
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES535511A Expired ES8602139A1 (es) | 1983-08-31 | 1984-08-30 | Un metodo para producir una proteina heterologa madura. |
| ES543767A Expired ES8604305A1 (es) | 1983-08-31 | 1985-05-31 | Un metodo de hidrolizar celulosa |
| ES543766A Expired ES8604304A1 (es) | 1983-08-31 | 1985-05-31 | Un metodo de hidrolizar celulosa |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES535511A Expired ES8602139A1 (es) | 1983-08-31 | 1984-08-30 | Un metodo para producir una proteina heterologa madura. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES543766A Expired ES8604304A1 (es) | 1983-08-31 | 1985-05-31 | Un metodo de hidrolizar celulosa |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0137280B1 (es) |
| JP (2) | JPH0616709B2 (es) |
| AU (1) | AU589112B2 (es) |
| BR (1) | BR8404346A (es) |
| CA (1) | CA1338400C (es) |
| DE (2) | DE3485558D1 (es) |
| DK (1) | DK419584A (es) |
| ES (3) | ES8602139A1 (es) |
Families Citing this family (110)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI841500A0 (fi) * | 1984-04-13 | 1984-04-13 | Valtion Teknillinen | Foerfarande foer uppbygnande av cellulolytiska jaeststammar. |
| US5320960A (en) * | 1992-04-03 | 1994-06-14 | Genencor International, Inc. | Method of preparing solution enriched in xylanase using low molecular weight alcohol, organic salt and inorganic salt |
| GB8610600D0 (en) * | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| US5989870A (en) * | 1986-04-30 | 1999-11-23 | Rohm Enzyme Finland Oy | Method for cloning active promoters |
| US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
| GB8621626D0 (en) * | 1986-09-08 | 1986-10-15 | Health Lab Service Board | Phenylalanine ammonia lyase |
| JP2507345B2 (ja) * | 1986-09-16 | 1996-06-12 | 三井東圧化学株式会社 | L―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子 |
| US5610034A (en) * | 1987-04-29 | 1997-03-11 | Alko Group Ltd. | Immunoglobulin production by trichoderma |
| US5688290A (en) * | 1989-10-19 | 1997-11-18 | Genencor International, Inc. | Degradation resistant detergent compositions based on cellulase enzymes |
| CA2069962A1 (en) * | 1990-08-17 | 1992-02-18 | Shi-Hsiang Shen | Recombinant dna production of beta-1, 3-glucanase |
| CA2093422C (en) * | 1990-10-05 | 2001-04-03 | Genencor International, Inc. | DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS |
| WO1992006221A1 (en) * | 1990-10-05 | 1992-04-16 | Genencor International, Inc. | Methods for treating cotton-containing fabrics with cellulase |
| US5328841A (en) * | 1990-10-05 | 1994-07-12 | Genencor International, Inc. | Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol |
| US5290474A (en) * | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
| US5650322A (en) * | 1990-10-05 | 1997-07-22 | Genencor International, Inc. | Methods for stonewashing fabrics using endoglucanases |
| US5997913A (en) * | 1990-12-10 | 1999-12-07 | Genencor International, Inc. | Method enhancing flavor and aroma in foods by overexpression of β-glucosidase |
| US5443750A (en) * | 1991-01-16 | 1995-08-22 | The Procter & Gamble Company | Detergent compositions with high activity cellulase and softening clays |
| FI945122L (fi) * | 1992-05-01 | 1994-10-31 | Genencor Int | Menetelmiä puuvillakankaiden käsittelemiseksi CBH I:n suhteen rikastetulla sellulaasilla |
| AU4712193A (en) * | 1992-08-19 | 1994-03-15 | Alko Group Limited | Fungal promoters active in the presence of glucose |
| WO1994026880A1 (en) * | 1993-05-10 | 1994-11-24 | Gist-Brocades N.V. | Combined action of endoglucanases and cellobiohydrolases |
| US6268196B1 (en) | 1993-12-17 | 2001-07-31 | Genencor International, Inc. | Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions |
| US7005128B1 (en) | 1993-12-17 | 2006-02-28 | Genencor International, Inc. | Enzyme feed additive and animal feed including it |
| US5861271A (en) * | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
| GB9400623D0 (en) * | 1994-01-14 | 1994-03-09 | Univ Leeds | Exploitation of the cellulase enzyme complex of neurospora |
| US5912157A (en) * | 1994-03-08 | 1999-06-15 | Novo Nordisk A/S | Alkaline cellulases |
| DE69536145D1 (de) * | 1994-03-08 | 2011-04-07 | Novozymes As | Neuartige alkalische Zellulasen |
| US7816129B2 (en) | 1994-07-29 | 2010-10-19 | Ab Enzymes Gmbh | Production and secretion of proteins of bacterial origin in filamentous fungi |
| US6001595A (en) * | 1996-11-29 | 1999-12-14 | Rohm Enzyme GmbH | Promoters and uses thereof |
| US6558937B1 (en) | 1997-07-31 | 2003-05-06 | Dsm N.V. | Cellulose degrading enzymes of aspergillus |
| EP0897667B2 (en) | 1997-07-31 | 2008-11-12 | DSM IP Assets B.V. | Bread improving composition |
| CA2345046A1 (en) * | 1998-10-13 | 2000-04-20 | Genesis Research And Development Corporation Limited | Materials and methods for the modification of plant cell wall polysaccharides |
| WO2001004284A1 (en) * | 1999-07-13 | 2001-01-18 | Midwest Research Institute | Cellobiohydrolase reduced glycosylation variants: cbhin45a; cbhin270a; and cbhin384a |
| US7375197B2 (en) | 2002-01-14 | 2008-05-20 | Midwest Research Institute | Cellobiohydrolase I gene and improved variants |
| US8637293B2 (en) | 1999-07-13 | 2014-01-28 | Alliance For Sustainable Energy, Llc | Cellobiohydrolase I enzymes |
| JP2004525109A (ja) | 2001-02-15 | 2004-08-19 | キング・ファーマシューティカルズ・インコーポレイティッド | 安定化された甲状腺ホルモン医薬組成物、及びその製法 |
| US7101569B2 (en) | 2001-08-14 | 2006-09-05 | Franz G Andrew | Methods of administering levothyroxine pharmaceutical compositions |
| EP1495055B1 (en) | 2002-04-18 | 2013-08-14 | Genencor International, Inc. | Production of functional antibodies in filamentous fungi |
| US7713725B2 (en) | 2002-09-10 | 2010-05-11 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| WO2004072225A2 (en) | 2003-02-12 | 2004-08-26 | Ramot At Tel Aviv University Ltd. | Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi |
| EP2311942A1 (en) | 2003-03-21 | 2011-04-20 | Genecor International, Inc. | Novel cbh1 homologs and variant cbh1 cellulases |
| JP5427342B2 (ja) | 2003-04-01 | 2014-02-26 | ジェネンコー・インターナショナル・インク | 変異体フミコーラ・グリセアcbh1.1 |
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| DK2333045T3 (en) | 2005-04-12 | 2017-02-20 | Danisco Us Inc | GEN-INACTIVATED MUTANTS WITH CHANGED PROTEIN PRODUCTION |
| US8198046B2 (en) | 2006-07-11 | 2012-06-12 | Danisco Us Inc. | KEX2 cleavage regions of recombinant fusion proteins |
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| US8143046B2 (en) | 2007-02-07 | 2012-03-27 | Danisco Us Inc., Genencor Division | Variant Buttiauxella sp. phytases having altered properties |
| DK2147104T3 (en) | 2007-05-21 | 2015-12-14 | Danisco Us Inc | USING A ASPARAGINPROTEASE- (NSP24) SIGNAL SEQUENCE FOR heterologous protein EXPRESSION |
| DK2235190T3 (en) | 2007-12-13 | 2018-07-23 | Danisco Us Inc | COMPOSITIONS AND PROCEDURES FOR PRODUCING ISOPRENE |
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| MY169163A (en) | 2008-09-15 | 2019-02-19 | Danisco Us Inc | Increased isoprene production using the archaeal lower mevalonate pathway |
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| US8993275B2 (en) * | 2013-02-12 | 2015-03-31 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| US8771994B1 (en) * | 2013-02-12 | 2014-07-08 | Novozymes Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| US8778641B1 (en) * | 2013-02-12 | 2014-07-15 | Novozymes Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| US8778640B1 (en) * | 2013-02-12 | 2014-07-15 | Novozymes Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| BR112015023537B1 (pt) | 2013-03-15 | 2022-11-29 | Inpex Corporation | Mutante de um microrganismo produtor de celulase,e, método para produção de uma celulase |
| CA2917690A1 (en) * | 2013-07-26 | 2015-01-29 | Biomethodes | Novel variant trichoderma reesei endoglucanases |
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| WO2018169780A1 (en) | 2017-03-15 | 2018-09-20 | Dupont Nutrition Biosciences Aps | Methods of using an archaeal serine protease |
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| CA3064574A1 (en) | 2017-06-14 | 2018-12-20 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
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| CA3080512A1 (en) | 2017-11-07 | 2019-05-16 | Codexis, Inc. | Transglutaminase variants |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4275163A (en) * | 1978-11-20 | 1981-06-23 | The United States Of America As Represented By The Secretary Of The Army | Cellulase-producing microorganism |
-
1984
- 1984-08-21 CA CA 461442 patent/CA1338400C/en not_active Expired - Lifetime
- 1984-08-29 DE DE8484110305T patent/DE3485558D1/de not_active Expired - Lifetime
- 1984-08-29 DE DE1984110305 patent/DE137280T1/de active Pending
- 1984-08-29 EP EP84110305A patent/EP0137280B1/en not_active Expired - Lifetime
- 1984-08-30 ES ES535511A patent/ES8602139A1/es not_active Expired
- 1984-08-30 AU AU32530/84A patent/AU589112B2/en not_active Ceased
- 1984-08-30 BR BR8404346A patent/BR8404346A/pt unknown
- 1984-08-31 JP JP59180893A patent/JPH0616709B2/ja not_active Expired - Fee Related
- 1984-08-31 DK DK419584A patent/DK419584A/da not_active Application Discontinuation
-
1985
- 1985-05-31 ES ES543767A patent/ES8604305A1/es not_active Expired
- 1985-05-31 ES ES543766A patent/ES8604304A1/es not_active Expired
-
1993
- 1993-10-07 JP JP5251285A patent/JP2584186B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES8604305A1 (es) | 1986-01-16 |
| EP0137280A1 (en) | 1985-04-17 |
| JPH0751071A (ja) | 1995-02-28 |
| ES535511A0 (es) | 1985-11-16 |
| CA1338400C (en) | 1996-06-18 |
| EP0137280B1 (en) | 1992-03-11 |
| DE3485558D1 (de) | 1992-04-16 |
| JPS60149387A (ja) | 1985-08-06 |
| ES8602139A1 (es) | 1985-11-16 |
| JP2584186B2 (ja) | 1997-02-19 |
| ES543766A0 (es) | 1986-01-16 |
| DK419584A (da) | 1985-03-01 |
| DK419584D0 (da) | 1984-08-31 |
| DE137280T1 (de) | 1986-01-16 |
| AU589112B2 (en) | 1989-10-05 |
| ES8604304A1 (es) | 1986-01-16 |
| BR8404346A (pt) | 1985-07-30 |
| JPH0616709B2 (ja) | 1994-03-09 |
| AU3253084A (en) | 1985-03-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent lapsed |
Effective date: 19960401 |