CA2345046A1 - Materials and methods for the modification of plant cell wall polysaccharides - Google Patents

Materials and methods for the modification of plant cell wall polysaccharides Download PDF

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Publication number
CA2345046A1
CA2345046A1 CA002345046A CA2345046A CA2345046A1 CA 2345046 A1 CA2345046 A1 CA 2345046A1 CA 002345046 A CA002345046 A CA 002345046A CA 2345046 A CA2345046 A CA 2345046A CA 2345046 A1 CA2345046 A1 CA 2345046A1
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dna
sequence
pinus radiata
sequences
plant
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French (fr)
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Leonard Nathan Bloksberg
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Rubicon Forests Holdings Ltd
Genesis Research and Development Corp Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Abstract

Novel isolated polynucleotides and polypeptides associated with the synthesis of plant cell wall polysaccharides are provided, together with genetic constructs comprising such sequences. Methods for using such constructs for the modulation of polysaccharide content in plants are also disclosed, together with transgenic plants comprising such constructs.

Description

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COMPREND PLUS D'UN TOME.
CECI EST LE TOME _ y -DE ~, NOTE: Pour tes tomes additionets, veuittez co~tacter to Bureau canadien des brevets JUMBO APPLfCATIONS/PATENTS
THIS SECTION OF THE APPlICATION/PATENT CONTAINS MORE
THAN ONE VOLUME
THIS lS VOLUME ~OF
_. . - _ PIOTE: For additional votumes-ptease contact-the Canadian Patent Office . . .. . . . _ .. . .. . . , . . ,. . ... . . . . ., . . ....,... .,. . , .;~a ... ..
<210> 724 <211> 238 <212> DNA
<213> Eucalyptus grandis <400> 724 gacgagacac cgatccgcgt gcacaccaac ttggagcacc ggggcatccc gtacccgaag 60 gaccagccca tgggcgtcta cagctcgata tggaacgccg acgactgggc cacccagggc 120 ggccgcatca agaccgactg gacccacgcc cccttcatca cgtcctaccg taacttcgag 180 atcgacgcgt gcgagtgccc ggcgaccatg gcggcggcag acaacgccaa gcggtgca 238 <210> 725 <211> 453 <212> DNA
<213> Eucalyptus grandis <400> 725 tggtggcggccgcggcggcctccggcaacttcaacaaggacttcgacatcacgtggggtg 60 atggccgtgcgcagatacccagcagtggccagctcctcacgctgtccctggacaagacgt 120 cggggtcgggcttccggtccaagaagcagtacttgttcgggaagattgacatgcagctca 180 aactcgtgcctgggaactccgccggcaccgtcaccgcttattacctttcttctttgggtt 240 ctgcgcacgacgaaatcgacttcgagtttctcggtaacctgagcggcgacccgtacaccc 300 tccatacgaacgtgttcacgcaaggaaaaggaaacagagaacaacagttccatctctggt 360 tcgaccccaccaaggacttccacacctactccatcctgtggaaccctcaaagcatcatct 420 tctctgtcgatggaacgcccataagagagttca 453 <210> 726 <211> 334 <212> DNA
<213> Eucalyptus grandis <400> 726 gccgatccgggtgttcaagaacagcaaggaccttggggtgaaattccccttcaaccagcc 60 gatgaaattgtactccagcctgtggaatgcggatgactgggccactcggggagggcttga 120 gaagaccgactggtccaaggcgccgttcgtggcctcttaccgggggttccacatcgacgg 180 gtgcgaagcgtcggttgaggccaagttctgcgctactcagggccagaggtggtgggacca 240 gaaggagttccaggacctcgatgccttccagtaccggaggctccggtgggtgcgctcgaa 300 atacaccatctacaactactgcgctgtcggaaga 334 <210> 727 <211> 416 <212> DNA
<213> Eucalyptus grandis <400> 727 accgtcaccgcatactatttatcttcaaacgggtcggcgtgggatgagatagacttcgag 60 ttcttggggaacttgagcggcgatccatacattctccacaccaacgtcttcagccaaggc 120 aagggtaaccgagagcagcaattctatctctggttcgacccaactgctgatttccacacc 180 tactccatcctctggaatccacaacgcatcatgtaagagtctgaaaagctcaaaatgcga 240 ccttacttgcatttacattgcgctacactattctctgcggcatttactcaaatacctttg 300 tttgcgatcattgcagattctcagtggacgggactcccatcagagagttcaagaacgcag 360 agtccatcggtgttcctttcccaaggcccagcccatgaggatattcttcgagccct 416 <210> 728 <211> 375 <212 > DNA
<213> Eucalyptus grandis <400>

acaaaacgcgcccaatatggcctctcgttccacttcttcgctttgtagagctgcgcttct 60 tctcatggtcgtttcttgggcgacttctgcttccgcccgcaacttctatcaagacttcga 120 cataacctggggagatggccgaggtcagatcctcaacaatggcgacctcctcactctctc 180 ccttgacaaggcctccggctccggcttccagtccaagaacgagtacttgttcggcaagat 240 tgacatgcagctcaaacttgttcctggcaactctgctggcaccgtcaccgcatactattt 300 atcttcaaatgggtcgacgtgggacgagatagatttcgagttcttggggaatttaagtgg 360 cgatccgtacattct 375 <210> 729 <211> 538 <212> DNA
<213> Eucalyptus grandis <400>

agaaggagtacctgttcggccggatcgacatgcagctcaagctcgtcgccgggaactccg60 ccggcaccgtgaccgcttactacttgtcttcgcaagggccaactcacgatgagattgact120 tcgagttcctggggaacctgagcggcgacccttacatcctccacaccaacgtcttcactc180 aagggaagggcaacagggagcagcagttctacctgtggtttgaccccaccagaaatttcc240 acacatactccgtcatctggaagccccagcacatcatcttcttggtagacaacattccta300 ttagagttttcaagaatggagagtcaattggcgtgcccttccccaagaaccagcccatga360 aaatatactcgagcctctggaatgccgatgattgggccacgagaggcggactgatcaaga420 cagactggtcgaaatcgcccttcacggcatactacaggaagttccaggccactgcctgca480 cctggtccacgggctcgtcctcctgtgagatcggacggcccgcttcctactctggatc 538 <210> 730 <211> 412 <212> DNA
<213> Eucalyptus grandis <400> 730 ccggatcgacatgcagctcaagctcgttgccgggaactccgccggcaccgtgaccgctta 60 ctacttgtcttcgcaagggccaactcacgacgagatcgacttcgagttcctggggaacct 120 gagcggcgacccttacatcctccacaccaacgtcttcactcaagggaagggcaacaggga 180 gcagcagttctacctgtggtttgaccccaccaggaatttccacacatactccgtcatctg 240 gaagccccagcacatcatcttcttggtagacaacattcctattagagttttcaagaatgg 300 agagtcaattggcgtgcccttccccaagaaccagcccatgaaaatatactcgagcctctg 360 gaatgccgatgattgggccacgagaggcggctgatcaagacagactggtcga 412 <210> 731 <211> 350 <212> DNA
<213> Eucalyptus grandis <400> 731 acaccgatcc gcgtgcacac caacttggag caccggggca tcccgtaccc gaaggaccag 60 cccatgggcg tctacagctc gatatggaac gccgacgact gggccaccca gggcggccgc 120 atcaagaccg actggaccca cgcccccttc atcacgtcct accgtaactt cgagatcgac 180 gcgtgcgagt gcccggcgac catggcggcg gcagacaacg ccaagcggtg cagcagcgcc 240 ggcagggaga ggcggtactg gtgggacgag cccacggtgt ccgagctgag cctccaccag 300 aaccaccagc tcaagtgggt ccaggcccac cacatggtct acgactactg 350 <210> 732 <211> 354 <212> DNA
<213> Eucalyptus grandis <400>

ctcctgtcgctgtccctcgacagagtgtcggggtcgggcttccggtccaagaaggagtac 60 ctgttcggccggatcgacatgcagctcaagctcgtcgccgggaactccgccggcaccgtg 120 accgcttactacttgtcttcgcaagggccaactcacgatgagattgacttcgagttcctg 180 gggaacctgagcggcgacccttacatcctccacaccaacgtcttcactcaagggaagggc 240 aacagggagcagcagttctacctgtggtttgaccccaccagaaatttccacacatactcc 300 gtcatctggaagccccagcacatcatcttcttggtagcaacattcctattagag 354 <210> 733 <211> 480 <212> DNA
<213> Eucalyptus grandis <400>

cccacatctctccctcctcttcagtcctcactctcatatcatctttgcttctcttcagca 60 actacagcagcagcagaaagacagacagaacacgcccaatatggccttcccttctacttc 120 ctcactttgcatcactacactgcttctcgtggtcgtttcttgggcgacgtctgcttccgc 180 ccgcaacttctatcaagacttcgacataacctggggtgatggccgagctcagatcctcaa 240 caacggcgacctcctcactctctcccttgacaaggcctccggctctggcttccagtccaa 300 gaacgagtacttgttcggcaagattgacatgcagctcaagctcgttcccggcaactccgc 360 aggcactgtcaccgcatactatttatcttcaaatgggtcgacgtgggacgagatagactt 420 cgagttcttggggaacttgagcggcgatccatacattcttcacaccaacgtgttcagcca 480 <210> 734 <211> 343 <212> DNA
<213> Eucalyptus grandis <400>

ccgcgtcaaggtactggataacggccagctcgtcaccctttccctggacaaggcctctgg 60 ctccggcttcaagtccaagaaccagtacctctatggcaaattcgacatgcaaatcaagct 120 cgtccccggcaactccgcgggcactgtcaccaccttatacttacattcggacgggtcgac 180 ctgggatgagatagacttggagttcttggggaacttgagcggcgacccttatatcctcca 240 caccaacttgtacagccaaggcaaaggcaatagggagcagcaattctatctctggttcga 300 cccgacagctgatttccacacttactccgtcctttggaaccct 343 <210> 735 <211> 359 <212> DNA
<213> Eucalyptus grandis <400> 735 atccaaggggtcttacctgttcgggcatttcagcatggacatcaagttggttgctggaga 60 ttctgcagggacagtcactgcattctacctctcatctcaaaactcagagcacgacgaaat 120 agactttgagttcttgggtaacaggagtgggcagccgtacatagtgcagaccaatgtgtt 180 cacgggaggaaaaggagacagagaacagaggatttatctttggttcgatcccaccgccgc 240 ctaccactcctactctgttctctggaacatgtaccagattgtattcttcgtggatgacgt 300 gccgatccgggtgttcaagaacagcaaggaccttggggtgaaattccccttcaaccagc 359 <210> 736 <211> 360 <212> DNA
<213> Eucalyptus grandis <400> 736 gcatactatt tatcttcaaa tgggtcaacg tgggacgaga tagacttcga gttcttgggg 60 aacttgagcggcgatccctacattcttcacaccaacgtgttcagccaaggcaagggcaac 120 cgagagcagcaattctatctctggttcgacccaactgctgatttccacacctactccatc 180 ctctggaatccacaacgcatcatattctcagtggacgggactccgatcagagagttcaag 240 aacgcagagtccatcggtgttcctttccccaaggcccagcccatgaggatattctcgagc 300 ctctggaacgcagacgactgggcgaccagaggcggcctagtgaagacggacttggacgca 360 <210> 737 <211> 437 <212> DNA
<213> Eucalyptus grandis <400> 737 gcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttcttcttcttca 60 atggctgtcctggtcttttccaaagtgtctgtgtcgttcggcctgttcgtgggtcttgcg 120 ttgttggtgggtctggtcgcgggtgcgaggtttgaggagctctaccagccgggctgggct 180 atggaccattttgtctacgaaggagaggttctcaagctcaagcttgacaactactctggc 240 gctgggttcgggtcgaagagcaagtacatgttcggcaaagttaccatccagatcaagctc 300 gtcgagggcgactcggctgggaccgtcactgctttctacatgtcgtcggatggaccgaac 360 cacaacgaattcgacttcgagttcctgggcaacacgacaggggagccctacctggtacag 420 accacgtgtccgtgacg 437 <210> 738 <211> 341 <212> DNA
<213> Eucalyptus grandis <400> 738 ggcgacccttacatcctccacaccaacgtcttcactcaagggaagggcaacagggagcag 60 cagttctacctgtggtttgaccccaccaggaatttccacacatactccgtcatctggaag 120 ccccagcacatcatcttcttggtagacaacattcctattagagttttcaagaatggagag 180 tcaattggcgtgcccttccccaagaaccagcccatgaaaatatactcgagcctctggaat 240 gccgatgattgggccacgagaggcggactgatcaagacagactggtcgaaagcgcccttc 300 acggcatactacaggaagttccaggccactgcctgcacctg 341 <210> 739 <211> 497 <212> DNA
<213> Eucalyptus grandis <400> 739 gagcttgctgctgatggctgccgnttcgcccgcagctgggaacttctaccaggacttcga 60 cctgacgtggggtggcagcgaccgcgccaagatcttcagcgggggtcagctcctgtcgct 120 gtccctcgacagagtgtcggggtcgggcttccggtccaagaaggagtacctgttcggccg 180 gatcgacatgcagctcaagctcgttgccgggaactccgccggcaccgtgaccgcttacta 240 cttgtcttcgcaagggccaactcacgacgagatcgacttcgagttcctggggaacctgag 300 cggcgacccttacatcctccacaccaacgtcttcactcaagggaagggcaacagggagca 360 gcagttctacctgtggtttgaccccaccaggaatttccacacatactccgtcatctggaa 420 gccccagcacatcatcttcttggtagacaacattcctattagagttttcaagaatggaga 480 gtcaattggcgtgccct <210> 740 <211> 497 <212> DNA
<213> Eucalyptus grandis <400> 740 ccaagaagga gtacctgttc ggccggatcg acatgcagct caagctcgtc gccgggaact 60 ccgccggcaccgtgaccgcttactacttgtcttcgcaagggccaactcacgatgagattg 120 acttcgagttcctggggaacctgagcggcgacccttacatcctccacaccaacgtcttca 180 ctcaagggaagggcaacagggagcagcagttctacctgtggtttgaccccaccagaaatt 240 tccacacatactccgtcatctggaagccccagcacatcatcttcttggtagacaacattc 300 ctattagagttttcaagaatggagagtcaattggcgtgcccttccccaagaaccagccca 360 tgaaaatatactcgagcctctggaatgccgatgattgggccacgagaggcggactgatca 420 agacagactggtcgaaatcgccttacggatactacagaagttcaggccactgctgacctg 480 gccacgggctgtctctg 497 <210> 741 <211> 395 <212> DNA
<213> Eucalyptus grandis <400> 741 cgcaacttttatcaagactttttcataacctgggggagatggccgagctcagatcctcaa 60 caatggcgacctcctcactctctcccttgacaaggcctccggctccggcttccagtccaa 120 gaacgagtacttgttcggcaagattgacatgcagctcaagctcgttcctggcaactccgc 180 aggcactgtcaccgcatactatttatcttcaaatgggtcaacgtgggacgagattgactt 240 cgagttcttggggaacttgagcggcgatccctacattcttcacaccaacgtgttcagcca 300 aggcaagggcaaccgagagcagcaattctatctctggttcgacccaactgctgatttcca 360 cacctactccatcctctggaatccacaacgcatta 3g5 <210> 742 <211> 396 <212> DNA
<213> Eucalyptus grandis <400>

ggcggccgaggaagaagggcaagtggaggctccgttggaccagacgcaagcgctcgcgtt 60 gaagccgctgaaggaggcggtgaaaggcgcctgcgaccagtcagtcttcacgaggccgcc 120 ccgggtggcccagtcctcagcgtcccatagggtcgagtagagggtcatgggctggctctt 180 tgggtaggggacccccgccgcatccaagttcttgaactcccttattggaatcccatcgac 240.

aaagtacctgttttttttagcagaaagagatccgtcattcattatgtggcgaaggacctc 300 aaatcggtgttattttagaaggaaatttatttgtcaaaggttttcatgatcaggacgagg 360 agtacttacacgacgtgtaaagggttccaaaggacg 396 <210> 743 <211> 347 <212> DNA
<213> Eucalyptus grandis <400> 743 caccacctta tacttacatt cggacgggtc gacctgggat gagatagact tggagttctt 60 ggggaacttg agcggcgacc cttatatcct ccacaccaac ttgtacagcc aaggcaaagg 120 caatagggag cagcaattct atctctggtt cgacccgaca gctgatttcc acacttactc 180 cgtcctttgg aaccctttac acgtcgtgta ctttgtcgat gggattccaa taagggagtt 240 caagaacttg gatgcggcgg gggtccccta cccaaagagc cagcccatga ccctctactc 300 gaccctatgg gacgctgagg actgggccac ccggggcggc ctcgtga 347 <210> 744 <211> 446 <212> DNA
<213> Eucalyptus grandis <400> 744 gtcaaggtac tggataacgg ccagctcgtc accctttccc tggacaaggc ctctggctcc 60 ggcttcaagtccaagaaccagtacctctatggcaaattcgacatgcaaatcaagctcgtc 120 cccggcaactccgcgggcactgtcaccaccttatacttacattcggacgggtcgacctgg 180 gatgagatagacttggagttcttggggaacttgagcggcgacccttatatcctccacacc 240 aacttgtacagccaaggcaaaggcaatagggagcagcaattctatctctggttcgacccg 300 acagctgatttccacacttactccgtcctttggaaccctttacacgtcgtgtactttgtc 360 gatgggattccaataagggagttcaagaacttggatgcggcgggggtcccctacccaaag 420 agccagcccatgaccctctactcgac 446 <210> 745 <211> 439 <212> DNA
<213> Eucalyptus grandis <400>

ccagtctctctcccccactctttctcaagccaaaatataagccaaagtataagccaaagt 60 atagagcaatcatggcccatgaaggtggaggtcctagtgcttcctccatggtggtgctcg 120 tgagcttgctgctgatggctgccgcttcgcccgcagctgggaacttctaccaggacttcg 180 acctgacgtggggtggcagcgaccgggccaagatcttcagcgggggtcagctcctgtcgc 240 tgtccctcgacagagtgtcggggtcgggcttccggtccaagaaggagtacctgttcggcc 300 ggatcgacatgcagctcaagctcgtcgccgggaactccgccggcaccgtgaccgcttact 360 acttgtcttcgcaagggccaactcacgatgagattgacttcgagttcctggggaacctga 420 gcggngacccttacatcct 439 <210> 746 <211> 322 <212> DNA
<213> Eucalyptus grandis <400> 746 acgacgagatcgacttcgagttcctggggaacctgagcggcgacccttacatnctccaca 60 ccaacgtcttcactcaagggaagggcaacagggagcagcagttctacctgtggtttgacc 120 ccaccaggaatttccacacatactccgtcatctggaagccccagcacatcatcttcttgg 180 tagacaacattcctattagagttttcaagaatggagagtcaattggcgtgcccttcccca 240 agaaccagcccatgaaaatatactcgagcctctggaatgccgatgattgggccacgagag 300 gcggactgatcaagacagactg 322 <210> 747 <211> 433 <212> DNA
<213> Eucalyptus grandis <400>

tggacaaggcctctggctccggcttcaagtccaagaaccagtacctctatggcaaattcg 60 acacgcaaatcaagctcgtccccggcaactccgcgggcactgtcaccaccttatacttac 120 attcggacgggtcgacctgggacgagatagacttggagttcttggggaacttgagcggcg 180 acccttatatcctccacaccaacttgtacagccaaggcaaaggcaatagggagcagcaat 240 tctatctctggttcgacccgacagctgatttccacacttactccgtcctttggaaccctt 300 tacacgtcgtgtactttgtcgatgggattccaataagggagttcaagaacttggatgcgg 360 cgggggtcccctacccaaagagccagcccatgaccctctactcgaccctatgggacgctg 420 aggactgggccac 433 <210> 748 <211> 525 <212> DNA
<213> Eucalyptus grandis <400> 748 WO 00/22092 PC'T/IYZ99/00169 aacagagcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttcttct 60 tcttcttcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcctgttcgtg 120 ggtcttgcgttgttggtgggtctggtcgcgggtgcgaggt.ttgaggagctctaccagccg 180 ggctgggctatggaccattttgtctacgaaggagaggttctcaagctcaagcttgacaac 240 tactctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttaccatccag 300 atcaagctcgtcgagggcgactcggctgggaccgncactgccttctacatgtcgtcggat 360 ggaccgaaccacaacgaattcgacttcgagttcctgggcaacacgacaggggagccctac 420 ctggtacagaccaacgtgtacgtgaacggggtgggcaaccgggagcagaggctcggcctc 480 tggttcgaccccaccactgacttccactcctactccgtcctctgg 525 <210> 749 <211> 385 <212> DNA
<213> Eucalyptus grandis <400> 749 cgtgagcttgctgctgatggctgccgcttcgcccgcagctgggaacttctaccaggactt 60 cgacctgacgtggggtggcagcgaccgggccaagatcttcagcgggggtcagctcctgtc 120 gctgtccctcgacagagtgtcggggtcgggcttccggtccaagaaggagtacctgttcgg 180 ccggatcgacatgcagctcaagctcgtcgccgggaactccgccggcaccgtgaccgctta 240 ctacttgtcttcgcaagggccaactcacgacgagatcgacttcgagttcctggggaacct 300 gagcggcgacccttacatcctccacaccaacgtcttcactcaagggaagggcaacaggga 360 gcagcagttctacctgtgggttgac 385 <210> 750 <211> 519 <212> DNA
<213> Eucalyptus grandis <400>

caaacacccacagctcttcctctctctccctcagtgtctcctcttcagtcctcactctca 60 tatcatctcattggcatttgcatctcttcagcaactacagccgcagcagcagcagcaaaa 120 aacaaaacacgccccatatggcctctcgttccacttcttcgctttgtaccaccgcgcttc 180 ttctcctggtcgtttcttgggcagcatccgcttttgcccgcaacttctaccaagacttcg 240 acataacctggggagatggccgagctcagatcctcaatagtggcgacctcctcactcttt 300 ccctcgacaaggcctccggctccggcttccagtccaagaacgagtacctgtttggcaaga 360 ttgacatgcaactcaagctcgttcccggcaactccgcaggcaccgtcaccgcttactatt 420 tatcttcaaacgggtcgacgtgggacgagatagacttcgagttcttggggaacttgagcg 480 gcgatccatacattctccacaccaacgtcttcagccaag 519 <210> 751 <211> 342 <212> DNA
<213> Eucalyptus grandis <400>

cgtgagcttgctgctgatggctgccgcttcgcccgcagctgggaacttctaccaggactt 60 cgacctgacgtggggtggcagcgaccgggccaagatcttcagcgggggtcagctcctgtc 120 gctgtccctcgacagagtgtcggggtcgggcttccggtccaagaaggagtacctgttcgg 180 ccggatcgacatgcagctcaagctcgtcgccgggaactccgccggcaccgtgaccgctta 240 ctacttgtcttcgcaagggccaactcacgacgagatcgacttcgagttcctggggaacct 300 gagcggcgacccttacatcctccacaccaacgtcttcactca 342 <210> 752 <211> 416 <212> DNA
<213> Eucalyptus grandis <400> 752 gagaaacagagcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttc 60 ttcttcttcttcttcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcct 120 gttcgtgggtcttgcgttgttggtgggtctggtcgcgggtgcgaggtttgaggagctcta 180 ccagccgggctgggctatggaccattttgtctacgaaggagaggttctcaagctcaagct 240 tgacaactactctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttac 300 catccagatcaagctcgtcgagggcgactcggctgggaccgtcactgctttctacatgtc 360 gtcngatggaccgaaccacaacgaattcgacttcgagttcctgggcaacacgaaca 416 <210> 753 <211> 408 <212> DNA
<213> Eucalyptus grandis <400> 753 agcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttcttcttcttc 60 ttcttcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcctgttcgtggg 120 tcttgcgttgttggtgggtctggtcgcgggtgcgaggtttgaggagctctaccagccggg 180 ctgggctatggaccattttgtctacgaaggagaggttctcaagctcaagcttgacaacta 240 ctctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttaccatccagat 300 caagctcgtcgagggcgactcggctgggaccgtcactgctttctacatgtcgtcggatgg 360 accgaaccacaacgaattcgacttcgagttcctgggcaacacgaacag 408 <210> 754 <211> 401 <212> DNA
<213> Eucalyptus grandis <400> 754 gagaaacagagcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttc60 ttctcttcttcttcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcctg120 ttcgtgggtcttgcgttgttggtgggtctggtcgcgggtgcgaggtttgaggagctctac180 cagccgggctgggctatggaccattttgtctacgaaggagaggttctcaagctcaagctt240 gacaactactctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttacc300 atccagatcaagctcgtcgagggcgactcggctgggaccgtcactgctttctacatgtcg360 tcggatggaccgaaccacaacgaattcgacttcgagttctg 401 <210> 755 <211> 403 <212> DNA
<213> Eucalyptus grandis <400>

gcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttcttcttcttct 60 tcttcaatggctgtcccggtcttttccaaagtgtctgtgtcnttcggcctgttcgtgggt 120 cttgcgttgttggtgggtctggtcgcgggtcaaggtttgaggagctctaccagccgggct 180 gggctatggaccattttgtctacgaaggagaggttctcaagctcaagcttgacaactact 240 ctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttaccatccagatca 300 agctcgtcgagggcgactcggctgggaccgtcactgctttctacatgtcgtcggatggac 360 cgaaccacaacgaattcgacttcgagttcctgggcacacgaca 403 <210> 756 <211> 414 <212> DNA
<213> Eucalyptus grandis <400> 756 gagaaacagagcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttc 60 ttcttcttcttcttcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcct 120 gttcgtgggtcttgcgttgttggtgggtctggtcgcgggtgcgaggtttgaggagctcta 180 ccagccgggctgggctatggaccattttgtctacgaaggagaggttctcaagctcaagct 240 tgacaactactctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttac 300 catccagatcaagctcgtcgagggcgactcggctgggaccgtcactgctttctacatgtc 360 gtcggatggaccgaaccacaacgaattcgacttcgagttcctggcaacacgaca 414 <210> 757 <211> 441 <212> DNA
<213> Eucalyptus grandis <400>

tgaaaatatactcgagcctctggaatgccgatgattgggccacgagaggcggactgatca 60 agacagactggtcgaaagcgcccttcacggcatactacaggaagttccaggccaccgcct 120 gcacctggtccacgggctcgtcgtcctgcgagatcggacggcccgcttcctactctggat 180 ccacatggaaaatcaatgagctcgatgcctatggccggaggcggctcaggtgggtccaga 240 agtacttcatgatctacgactactgcgcggacggcaagaggttccctcaaggcatcccag 300 ccgaatgcaagcgctcgcgattctagaaaagaaaaagagggaaatcaacgtgttcgaatg 360 gaaggtcgctcggcagtatatagttaatgcattctttcgtttcgtttcgcatgttcaagt 420 catgacttgctcgtggtgtct 441 <210> 758 <211> 499 <212> DNA
<213> Eucalyptus grandis <400>

gcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttcttcttcttct 60 tcttcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcctgttcgtgggt 120 cttgcgttgttggtgggtctggtcgcgggtgcgaggtttgaggagctctaccagccgggc 180 tgggctatggaccattttgtctacgaaggagaggttctcaagctcaagcttgacaactac 240 tctggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttaccatccagatc 300 aagctcgtcgagggcgactcggctgggaccgtcactgctttctacatgtcgtcggatgga 360 ccgaaccacaacgaattcgacttcgagttcctgggcaacacgacaggggagccctacctg 420 gtacagaccaacgtgtacgtgaacggggtgggcaaccgggaacagaggctcggcctctgg 480 ttcgaccccaccactgact 499 <210> 759 <211> 340 <212> DNA
<213> Eucalyptus grandis <400>

gttggcagggaagagagatatggcctctggttcgatccttccgatgacttccatcagtac 60 ggcatcctgtggaccgactctcaaatcatattttatgtggacaatgtacccatcagagag 120 tttaagagaacggaggctatgggaggccaattcccctctaaacctatgtctttatacgcc 180 acaatctgggatggctctgattgggccacaaatggcggaaaataccgagtgaactacaag 240 tacgccccctacgtcgccaagttctctgatcttgtcctgcacggctgcgcagttgacccg 300 attgagcaccaattaagctgcaacgatgtcccaagtcata 340 <210> 760 <211> 350 <212> DNA
<213> Eucalyptus grandis <40.0>

tcttggtagacaacattcctattagagttttcaagaatggagagtcaattggcgtgccct 60 tccccaagaaccagcccatgaaaatatactcgagcctctggaatgccgatgattgggcca 120 cgagaggcggactgatcaagacagactggtcgaaagcgcccttcacggcatactacagga 180 agttccaggccaccgcctgcacctggtccacgggctcgtcgtcctgcgagatcggacggc 240 ccgcttcctactctggatccacatggaaaatcaatgagctcgatgcctatggccggaggc 300 ggctcangtgggtccagaagtacttcatgatctacgactactgcgcggac 350 <210> 761 <211> 288 <212> DNA
<213> Eucalyptus grandis <400>

cttgtggacgagacaccgatccgcgtgcacaccaacttggagcaccggggcatcccgtac 60 ccgaaggaccagcccatgggcgtctacagctcgatatggaacgccgacgactgggccacc 120 cagggcggccgcatcaagaccgactggacccacgcccccttcatcacgtcctaccgtaac 180 ttcgagatcgacgcgtgcgagtgcccggcgaccatggcggcggcagacaacgccaagcgg 240 tgcagcagcgccggcagggagaggcggtactggtgggacgagcccacg 2ge <210> 762 <211> 364 <212> DNA
<213> Eucalyptus grandis <400>

ttgacttcgagttcctggggaacctgagcggcgacccttacatcctccacaccaacgtct 60 tcactcaagggaagggcaacagggagcagcagttctacctgtggtttgaccccaccagaa 120 atttccacacatactccgtcatctggaagccccagcacatcatcttcttggtagacaaca 180 ttcctattagagttttcaagaatggagagtcaattggcgtgcccttccccaagaaccagc 240 ccatgaaaatatactcgagcctctggaatgccgatgattgggccacgagaggcggactga 300 tcaagacagactggtcgaaatcgcccttcacggcatactacaggaagttccaggccactg 360 cctg 364 <210> 763 <211> 343 <212> DNA
<213> Eucalyptus grandis <400>

acgacaggggagccctacctggtacagaccaacgtgtacgtgaacggggtgggcaaccgg 60 gagcagaggctcggctctggttcgaccccaccactgacttccactcctactccgtcctct 120 ggaaccagcgccaagtcgtgtttcttgtggacgagacaccgatccgcgtgcacaccaact 180 tggagcaccggggcatcccgtacccgaaggaccagcccatgggcgtctacagctcgatat 240 ggaacgccgacgactgggccacccanggcggccgcatcaagaccgactggacccacgccc 300 ccttcatcacgtcctaccgtaacttcgagatcgacgcgtgcga 343 <210> 764 <211> 301 <212> DNA
<213> Eucalyptus grandis <400> 764 cgtcactgct ttctacatgt cgtcggatgg accgaaccac aacgaattcg acttcgagtt 60 cctgggcaac acgacagggg agccctacct ggtacagacc aacgtgtacg tgaacggggt 120 gggcaaccgg gagcagaggc tcggcctctg gttcgacccc accactgact tccactccta 180 WO 00/22092 PC1'/NZ99/OO169 ctccgtcctc tggaaccagc gccaagtcgt gtttcttgtg gacgagacac cgatccgcgt 240 gcacaccaac ttggagcacc ggggcatccc gtacccgaag gaccagccca tgggcgtcta 300 c 301 <210> 765 <211> 516 <212> DNA
<213> Eucalyptus grandis <400>

gcgacttcagagctcgtgaagaaaagcttttgctctcgctcttcttcttcttcttcttct 60 tcaatggctgtcccggtcttttccaaagtgtctgtgtcgttcggcctgttcgtgggtctt 120 gcgttgttggtgggtctggtcgcgggtgcgaggtttgaggagctctaccagccgggctgg 180 gctatggaccattttgtctacgaaggagaggttctcaagctcaagcttgacaactactct 240 ggcgctgggttcgggtcgaagagcaagtacatgttcggcaaagttaccatccagatcaag 300 ctcgtcgagggcgactcggctgggaccgtcactgccttctacatgtcgtcggatggaccg 360 aaccacaacgaattcgacttcgagttcctgggcaacacgacaggggagccctacctggta 420 cagaccaacgtgtacgtgaacggggtgggcaaccgggagcagagctcggcctctggttcg 480 accccaccactgacttcactcctactccgtcctctg 516 <210> 766 <211> 349 <212> DNA
<213> Eucalyptus grandis <400> 766 gtccctcgacagagtgtcggggtcgggcttccggtccaagaaggagtacctgttcggccg 60 gatcgacatgcagctcaagctcgtcgccgggaactccgccggcaccgtgaccgcttacta 120 cttgtcttcgcaagggccaactcacgatgagattgacttcgagttcctggggaacctgag 180 cggcgacccttacatcctccacaccaacgtcttcactcaagggaagggcaacagggagca 240 gcagttctacctgtggtttgaccccaccagaaatttccacacatactccgtcatctggaa 300 gccccagcacatcatcttcttggtagacaacattcctattagagttttc 349 <210> 767 <211> 479 <212> DNA
<213> Eucalyptus grandis <400>

gcgggttccaaacgaagcagagataccgattcgggtggttcagcatgaagctcaagctcg 60 tgggaggcgactcggccggagtggtcactgcttactacatgtgttcggagaacggggcag 120 ggccggagagggatgagctggacttcgagttcttggggaaccggagcggacagccgtatc 180 tgatccagaccaacgtgtacaagaacgggacgggctcgcgcgagatgcggcacatgctct 240 ggttcgacccgaccgaggacttccactcctattccatcctctggaacagccaccagattg 300 tgttcttcgtcgaccaggtgccggtcagggtgttcaagaacaacggcgaggcgaacaact 360 tcttccccaacgagaagcccatgtacctcttctcgagcatctggaacgccgacgactggg 420 ccacgaggggcggccttgagaagaccgactggaccaaggcgccgttcgtgtccacctac 479 <210> 768 <211> 371 <212> DNA
<213> Eucalyptus grandis <400> 768 gttcggcctg atcgaagtca atctcaccgt gaccgcttac tacttgtctt cgcaagggcc 60 aactcacgat gagattgact tcgagttcct ggggaacctg agcggcgacc cttacatcct 120 ccacaccaac gtcttcactc aagggaaggg caacagggag cagcagttct acctgtggtt 180 WO 00/22092 PC'T/NZ99/00169 tgaccccacc agaaatttcc acacatactc cgtcatctgg aagccccagc acatcatctt 240 cttggtaaca acatcaccca tctctctctc tctctccccc actctctctc aagccaaaat 300 ataagccaaa gtataagcca aagtatagag caatcatggc ccatgaaggt ggaggtccta 360 gtgcttcctc c <210> 769 <211> 368 <212> DNA
<213> Eucalyptus grandis <400> 769 gttcggccggatcgaagtcaatctcaccgtgaccgcttactacttgtcttcgcaagggcc 60 aactcacgatgagattgacttcgagttcctggggaacctgagcggcgacccttacatcct 120 ccacaccaacgtcttcactcaagggaagggcaacagggagcagcagttctacctgtggtt 180 tgaccccaccagaaatttccacacatactccgtcatctggaagccccagcacatcatctt 240 cttggtagacaacatcacccatctctctctctctctcccccactctctctcaagccaaaa 300 tataagccaaagtataagccaaagtatagagcaatcatggcccatgaaggtggaggtcct 360 agtgcttc <210> 770 <211> 342 <212> DNA
<213> Eucalyptus grandis <400>

ccagtccaagaacgagtacctgtttggcaagattgacatgcaactcaagctcgttcccgg 60 caactccgcaggcaccgtcaccgcttactatttatcttcaaacgggtcgacgtgggacga 120 gatagacttcgagttcttggggaacttgagcggcgatccatacattctccacaccaacgt 180 cttcagccaaggcaagggtaaccgagagcagcaattctatctctggttcgacccaactgc 240 tgatttccacacctactccatcctctggaatccacaacgcatcatattctcatggacggg 300 actcccatcagagagttcaagaacgcagagtccatcggcgtt 342 <210> 771 <211> 580 <212> DNA
<213> Eucalyptus grandis <400>

ctcctcttcagtcctcactctcatatcatctcattgagatttgcttctcttcagcaacta 60 cagcagcagcagcagcagacagaacacgcccaatatggcctccctttctacttcttcgct 120 tcgcatcgccactctgcttctcgtggtcgtttcttgggggacgtttgcttccgcccgcaa 180 cttctatcaagacttcgacataacctggggtgatggccgagctcagatcctcaacaacgg 240 cgacctcctcactctctccctcgacaaggcctccggctccggcttccagtccaagaacga 300 gtacttgttcggcaagattgacatgcagctcaagctagttcccggcaactccgctggcac 360 cgtcaccgcatactatttatcttcaaacgggtcggcgtgggatgagatagacttcgagtt 420 cttggggaacttgagcggcgatccatacattctccacaccaacgtcttcagccaaggcaa 480 gggtaaccgagagcagcaattctatctctggttcgacccaactgctgatttccacaccta 540 ctccatcctctggaatccacaacgcatcatattctcagtg 580 <210> 772 <211> 407 <212> DNA
<213> Pinus radiata <400> 772 agtagtgtgc ccttcacaac aattatgtcg ccagctgggg ctcagatcac atcaaacatt 60 ccatggcggt cgaaagatga gctgctcctc aacaaacagt atggtgcggg gtttgagtcg 120 aaggggacatatttatttgggcatttcagtatgcagataaagctggttgccggtgattcc 180 gctggcactgtcaccgccttttatctttcttctcagactgcagagcacgatgagatagac 240 tttgaattcttggggaacaagtcgggggaaccctacattcttcagaccaatgtatttacg 300 ggcgggaagggtgagagagagcaccgaatatacctctggttcgaccccaccaaggattac 360 cattcctatgcggtgctctggaacatgtcccaaattgcatttttggt 407 <210> 773 <211> 403 <212> DNA
<213> Pinus radiata <400> 773 tgacaatggacaagagttgcagcttactcttgaccgctcttcaggttgtggtattcaatc 60 caagcaagagtatctatttgccaagattgatatccaaatcaagttggtacctggcaactc 120 tgcaggcacagtcactaccttttatctatcatctcaaggtcccaaacacgacgaaataga 180 cttcgaatttctgggcaacctgtccggagatccttatattttgcacactaatgtctttgc 240 tcaaggccttggtgggcgtnagcagcaattttacttgtggttcgacccaaccctggattt 300 ccacacttactccgtgctctggacatcaaaccaaattatattttctgtagacgggagtct 360 attcgagtgtttaagaacagggagacagagtngggtaaagtgg 403 <210> 774 <211> 400 <212> DNA
<213> Pinus radiata <400>

tgacaatggacaagagttgcagcttactcttgaccgctcttcaggttgtggtattcaatc 60 caagcaagagtatctatttgccaagattgatatccaaatcaagttggtacctggcaactc 120 tgcaggcacagtcactaccttttatctatcatctcaaggtcccaaacacgacgaaataga 180 cttcgaatttctgggcaacctgtccggagatccttatattttgcacactaatgtctttgc 240 tcaaggccttggtgggcgtgagcagcaattttacttgtggttcgacccaaccctggattt 300 ccacacttactccgtgctctggacatcaaaccaaattatatttctgtagacgggagtcta 360 ttcgagtgtttaagaacagggagacagagttgggtaaagt 400 <210> 775 <211> 384 <212> DNA
<213> Pinus radiata <400>

gataactgttgttgataataatggcttgtttaagaatgcagagttgctgcttcttcattc 60 tggttttttgcttctgggtatctaattgtgcagagttcaatgatatcttcgagcccagct 120 gggcgattgatcatgttatgaacgagggagagctgttgaagctgaagctcgacaactttt 180 ctggcgctggcttttcttccaaggcaacatacttgtttggaaaagtaggggcgcagatta 240 aactcgttcccggcgactctgcgggcacgtgactgcattttatatgtcttctgaggggac 300 attgcatgacgaattcgatttcgaattcttgggaaatgcttcgggtgagccttacattgt 360 gcagactaatatctactcaatggg 384 <210> 776 <211> 345 <212> DNA
<213> Pinus radiata <400> 776 tgagaaacag cccatgaggg ggtcctcttc aatctggatg cagataactg ggctactcaa 60 ggtgggcggc tgaagataaa ctggggccat tctcctttta tctccactta caaaggcttc 120 aacattggtg caaacaaata cggattaaat ggagaaccaa gaggggttat taaaaatgga 180 agtaagtggt gggacaggcc ctctcattct tcccttactc cattacaaag gcgaatgctc 240 cgatgggtac atcggaacta tatcatctat gattactgca aggattcgac caggttttcc 300 acttcgccac ctgagtgtgc aggcctccgc ttctagttct ttata 345 <210> 777 <211> 449 <212> DNA
<213> Pinus radiata <400>

gttgggtagcaacgtatgcagataagctgttgttaaaatggcttctttgagtatgcagag 60 ctgcttcttaattctggctctttgcttctgggcatcccattgtgcacagtttaatgatat 120 cttcgagcccagctgggcgacagatcatgttatgtatgagggagagctgttgaagcttaa 180 gctggacaatatttccggggctggctttgcttccaagacaacatatttgtttggaaaagc 240 aggggcacagattaagctcgttccaggtgactctgcaggcacagttactgctttttatat 300 gtcttctgaggggactctgcacgacgaattcgatttcgaattcttgggaaatgcttcggg 360 tgagccttacattgtgcagacgaatatctactccaacggcactggcaaaagggaacaacg 420 tattacctctggttcgaccccacggcaga 449 <210> 778 <211> 354 <212> DNA
<213> Pinus radiata <400>

tattaatccataattatggacatgggcatgcccctcctctttcttttcttattaataacc 60 tcgtcgagtcttcttgtaactgtttctgcaaatttctacaacgatgtagatatcacatgg 120 ggcaatggtcgtggtaaaatccttgacaatggccaacaattacagcttactctggatcgc 180 acttcaggttgtgggtttcaatctaagaatgagtatctgtttgctaaaattgatatgcaa 240 ataaagttggtacctggcaactctgccggcacagttactgcctattatctgtcgtctcaa 300 ggttccgaacacgacgaaatagactatgaatttctaggcaacctgtctggagat 354 <210> 779 <211> 392 <212> DNA
<213> Pinus radiata <400> 779 ccggtctgttgttgtgggaaataggatggccgggaaaaggaattggttcaagagaatcga 60 gtttattgttatattcgtggtttgcttaaactctgtttctgcacgcccggcatcatttgc 120 agaggattttaaagtgacgtgggcagatgaccatgtcaaaacaaggtcagataacaactc 180 catcgatctcatcctggatcagaattcaggggcaggatttgcctccaagaatcagtacat 240 gtttggacttgtaagcatgaacatcaaacttgtggcgggtgattctgcagggacagtcac 300 tgctttttatatgagctcggacaaggaggaagtgcgagatgaattggatttcgagtttct 360 ggggacagatcaggacagccttatacagtcca 392 <210> 780 <211> 293 <212> DNA
<213> Pinus radiata <400> 780 cgtttattcaaaaggggttggcaacagagaacagcgctttttcttatggttcgacccaac 60 tgcagactttcattcctattcctttctgtggaatcgccaccaagttgttttctttgtgga 120 tgatgtgcccgtacggatattttccaacaatgagaaaagaggagtcccatatcctcaaac 180 tcaacccatgggcgtatactcgtcaatatggaacgcagacgattgggctactcaaggggg 240 cctcgtcaagaccgattggagccacgcacctttcatttccacatacaagaatt 293 <210> 781 <211> 451 <212> DNA
<213> Pinus radiata <400>

ctccaatggcactggcaacagggaacaacgcatttacctctggttcgaccccaccgcaga 60 tttccattcctattcttttctgtggaaccacaagcaagttgtattctttgtagacagtgt 120 tccgattagggtattccccaacaacgagaggctgggagtcccatatcctaagaaacagcc 180 catgagggtatcctcttcaatctggaatgcagataactgggctactcaaggtgggcggct 240 gaagataaactggagccattctccttttatctccacttacagaaggttcgacatcgatgc 300 aaaccaatacggattaaatggagaatcaagagggtttattgagaatggaagtaagtggtg 360 ggacaggccctctcattcttcccttactccattacaaaggcgaatgctcccgatgggtgc 420 atcggaactatatcatctatgcctacctgaa 451 <210> 782 <211> 387 <212> DNA
<213> Pinus radiata <400>

cactggcttagatacacagtccagtcgaatcgggtcttgaggatggcattcgtaggttgt 60 caagaagggggtcgtgtcatgcactgcgctcttctttgtatctttgtaacgttgtgcaac 120 ctgctcgtgagctctcaatgtgcgtccttcgacgatttcttctaccccagttgggctgtt 180 gatcatgtcatgtcccaaggagagttgctccagctcaagcttgataacatttctggtgca 240 ggatttgcttcgaagagcacatacatcttcggaaaagcaaatgtgcagattaagctcgtt 300 cccggggactctgctggcactgttactgcattctatatgtcttcccaaggcgatcagcat 360 gacgaattcgactttgaatttttgggg 387 <210> 783 <211> 401 <212> DNA
<213> Pinus radiata <400> 783 cgtttattcaaaaggggttggcaacagagaacagcgctttttcttatggttcgacccaac 60 tgcagactttcattcctattcctttctgtggaatcgccaccaagttgttttctttgtgga 120 tgatgtgcccgtacggatattttccaacaatgagaaaagaggagtcccatatcctcaaac 180 tcaacccatgggcgtatactcgtcaatatggaacgcagacgattgggctactcaaggggg 240 cctcgtcaagaccgattggagccacgcacctttcatttccacatacaagaatttcagcat 300 tgatgcctgtcaatattcctcgaaaacgagctgcgcttcgtggtgggatgagcctgctta 360 cgcttctcttgatggaaagcagaggctgaaactgaatgggt 401 <210> 784 <211> 370 <212> DNA
<213> Pinus radiata <400> 784 cttcagacca atgtatttac gggcgggaag ggtgagagag agcaccgaat atacctctgg 60 ttcgacccca ccaaggatta ccattcctat gctgtgctct ggaacatgta ccaaattgca 120 tttttggtan atgaggtacc aatccgggtg ttcaagaaca gcaaggatct gggcgtgagg 180 tacccattta accagccgat gaagatctat tcgagcctgt ggaatgctga tgactgggcc 240 acccgagggg gtctggagaa aaccgactgg gccaaggcgc ccttcatcgc ctcctacagg 300 gaattccacg tcgatgcctg tgaggcttct gctccgaaat cggtgtgcgc aacgaagggg 360 gcggcggtgg 370 <210> 785 <211> 241 <212> DNA
<213> Pinus radiata <400> 785 atctactccaacggcactggcaaaagggaacaacgtatttacctctggttcgaccccacg 60 gcagatttccattcctattcttttctatggaaccacaaccaagttgttttctttgtggat 120 agtgttccgattcgggtattccccaacaacgagcggctgggagtcccatatccgaaaagc .180 cagccgctgagagtatcctcctcaatctggaatgcagccgactgggctacacaaggcggg 240 c 241 <210> 786 <211> 180 <212> DNA
<213> Pinus radiata <400> 786 catctactcc aacggcactg gcaaaaggga acaacgtatt tacctctggt tcgaccccac 60 ggcagatttc cattcctatt cttttctatg gaaccacaac caagttgttt tctttgtgga 120 tagtgttccg attcgggtat tccccaacaa cgagcggctg ggagtcccat atcccgaaaa 180 <210> 787 <211> 264 <212> DNA
<213> Pinus radiata <400> 787 ctggactttg agtttctggg gaacagaagt ggagagccct atgctctgca gacgaacatc 60 tatgcaaagg gtgtgggtgg cagggaacag aggcacatcc tctggttcga tccaacaaca 120 cagtttcaca cttactccat cctctggaac tctcatcaga ttgtgttctt cgtagaccaa 180 gttcctgtga gagtccacag gcacactgag gctacgagcg atgcgttccc taaagaacag 240 gggatgtaca tgttttccag catt 264 <210> 788 <211> 298 <212> DNA
<213> Pinus radiata <400> 788 ttccattttc cgcagaattc aaagcatggg gcatcttctc tactctctgg gaaagccgat 60 taactgggcg acgaggggcg ggctggaaaa gattgactgg agcaaggcgc cattcgttgc 120 ctcctacagg ggatttgaga tcgaatcctg ccagtacccg ggtaaagcga gctgcgtggt 180 taacaccagc aattggtggg aagggttgag ctacagcggc ctcaaaccaa atcaagcgag 240 gttatacaaa tgggtgagga cgaattacat gatttatgat tattgtaagg acacgccc 298 <210> 789 <211> 375 <212> DNA
<213> Pinus radiata <400> 789 tcgtaccagg agactctgca ggagttgtca ctgcttatta tatgtcttct gacacagaca 60 tgaataggga cgagctagat tttgaatttc tagggaacag aagtggacag ccttatggtc 120 ttcagacgaa catctattca aatggtgtgg gtggaaggga acagaggcat atcctctggt 180 tcgatccaac gacagagttt cacacttact ctatcctctg gaacgctcat cagattgtgt 240 ttttcgtgga ccaagttcca ttgagagtcc acaggcacac taaggctaca cgccatgtgt 300 tccctcgaaa gcaggggatg tacatgttct ccagcatttg gaatggagac aactgggcaa 360 cgagaaggcg gcctt 375 <210> 790 <211> 442 <212> DNA
<213> Pinus radiata <400> 790 tcgtaccaggagactctgcaggagttgtcactgcttattatatgtcttctgacacagaca 60 tgaatagggacgagctagattttgaatttctagggaacagaagtggacagccttatggtc 120 ttcagacgaacatctattcaaatggtgtgggtggaagggaacagaggcatatcctctggt 180 tcgatccaacgacagagtttcacacttactctatcctctggaacgctcatcagattgtgt 240 ttttcgtggaccaagtnccattgagagtccacaggcacactaaggctacacgccatgtgt 300 tccctcgaaagcaggggatgtacatgtnctccagcatttggaatggagacaactgggcaa 360 cgagaggcggcnttgagaagacccaacctgggcggctgctccgttcgtatcttcgtacaa 420 gaaattccatgggctcggtgca 442 <210> 791 <211> 424 <212> DNA
<213> Pinus radiata <400>

cctattcgggtgttttccaacaatgagaaaagaggagtcccatttcctcaaacccgccct 60 tatgggcgtatactcttcaatatggaacgcagatgactgggctactcaaggtggccgcgt 120 aaagaccgattggagccacgctccttttatttccacatacacaagtttcaacatcgatgc 180 ttgcaaatatagccctggcagttcatgtacttcgtggtgggatcagccggcgtacgcctc 240 acttaccgcaaagcagcgcatgcaactcaagtgggtacacgagaaatacatgatttacga 300 ttactgcaaagatccagttagatttggtacacctccagcagagtgtactgcatgatgtgg 360 gtctttaaacttgacttcctacccattccatgatcttaattcacaaatatttgtccactt 420 gaat 424 <210> 792 <211> 219 <212> DNA
<213> Pinus radiata <400> 792 ggcacatgat gcatttatat gtcttctgag gggacttgca tgacgattcg atttcgaatt 60 cttgggaaat gcttcgggtg agccttacat tgtgcagact aatatctact ccaacggcac 120 tggcgacagg gaacaacgca tttacctctg gttcgacccc accgcagatt tccattccta 180 t.tcttttctg tggaaccaca agcaagttgt attctttgt 219 <210> 793 <211> 405 <212> DNA
<213> Pinus radiata <400> 793 attcggcagagctctggggtccncagcaccaaacaatctggaatgatcagtctggaataa 60 ccatttggctcgacaggaactcgggaagcggatttaaatcgctgagaccgtacaagtctg 120 gctacttcagtgcagccattaagttgcaggctgggtatactgcaggagtcaatactgcct 180 tttatttatcgaacaacgaggctcacccggactaccacgatgaaatagacattgaatttc 240 tgggaaatatcccgggaagaccttataccttacagaccaacatttatgtaagagctggaa 300 atgcgggacgagggaggattattacaggaagggngcagcaaatccacctttggtttgatc 360 ccactaagga ttttcaccgt tacagcattc tctggactcc atttg 405 <210> 794 <211> 348 <212> DNA
<213> Pinus radiata <400>

ggaatggggagagctgggcaaccaacggaggaaagacaaagatcacctgggagccttacc 60 catttgttgctcagttcagaaacttttacatcgatggctgtgagtggaatggaaacccta 120 ggttctgcaagggaggcagcacacaaaattggtggaataagagaacatatgcttacttta 180 acgctggggatagactcaaactccattgggtaaggaagcattatctcgtctatgactact 240 gtaatgacaaggtcagattcaaagtagcccctgaggaatgcaggtaccacatttaatcca 300 tagcctgccgagcattttttagtatttggtaataagacagtctggtgg 348 <210> 795 <211> 377 <212> DNA
<213> Pinus radiata <400> 795 ctttctctgggaatcgccaccaatctgttttctttgtggacgatgtacctattcgggtgt 60 tttccaacaatgagaaaagaggagtcccatttcctcaaacccgccctatgggcgtatact 120 cttcaatatggaacgcagatgactgggctactcaaggtggccgcgtaaagaccgattgga 180 gccacgctccttttatttccacatacacaagtttcaacatcgatgcttgcaaatacagcc 240 ctggtagttcatgtacttcgtggtgggatcagccggcgtacgcctcacttaccgcaaagc 300 agatcatgcaactcaagtgggtacacgagaaatacatgatttacgattactgcaaagatt 360 cagttagatttggtaca 377 <210> 796 <211> 379 <212> DNA
<213> Pinus radiata <400> 796 gacatggcttgtttaagattgcagagctgctgctttttcgttctggttttttgcttctgg 60 gtatctaattgtgcagagttcaatgatatcttcgagcccagctgggcgattgatcatgtt 120 atgaacgagggagagctgttgaagctgaagctcgacaatttttctggcgctggcttttct 180 tccaaggccacatacttgtttggaaaagtaggggcgcagattaaactcgttcccggcgac 240 tctgcgggcacagtgactgcattttatatgtcttctgaggggacattgcatgacgaattc 300 gatttcgaattcttgggaaatgcttcgggtgaagccttacattgtgcagactaatatcta 360 ctccaaacgggaactggcg 379 <210> 797 <211> 315 <212> DNA
<213> Pinus radiata <400>

tagacattgaatttctgggaaatatcccgggaagaccttataccttacagaccaacattt 60 atgtaagagctggaaatgcgggacgagggaggattattacaggaagggagcagcaaatcc 120 acctttggtttgatcccactaaggattttcaccgttacagcattctctggactccattga 180 agattatattttttgtggatgacatcccaataagaaagtatcgaaggacgaacccttaca 240 ctttcccggcaaggcccatgtgggctgtacggatcgattctgggatgcatctccttgggc 300 tacagataacgggaa 315 <210> 798 WO 00/22092 PC'T/NZ99/00169 <211> 412 <212> DNA
<213> Pinus radiata <400>

ggaaactttgatgatgatttttacattatgtggtcacctgatcatgtcaccaggctctac 60 aatggacatggcgtgcagattagtatggacaggcagtcaggttcgggcttcgcaacgaag 120 aaacaatatttgtttggcaagttcgaaatgcaaatcaagcttccaccaggaaattctgcg 180 ggcactgttgtggctgtttatttgtattccaatcagccaaacagagacgagatcgacatt 240 gaatttttgggcaatgttgatggcaaggacatcatcatgcagactaatgtttttgctaat 300 ggctacgatgatcgagagcagcggatcaaactctggttggatcccacagcagattttcac 360 acatacacaatcttttggaaccgttaccacattgtatttctggtagatggct 412 <210> 799 <211> 303 <212> DNA
<213> Pinus radiata <400> 799 ggcagagctagattttgaatttctagggaacagaagtggacagccttatggtcttcagac 60 gaacatctattcaaatggtgtgggtggaagggaacagaggcatatcctctggttcgatcc 120 aacgacagagtttcacacttactctatcctctggaacgctcatcagattgtgtttttcgt 180 ggaccaagttccattgagagtccacaggcacactaaggctacacgccatgtgttccctcg 240 aaagcaggggatgtacatgttctccagcatttggaatggagacaactgggcaacgagagg 300 cgg 303 <210> 800 <211> 298 <212> DNA
<213> Pinus radiata <400>

cgtggaccaagttccattgtgtgtccacaggcacactaaggctacacgccatgtgttccc 60 tcgaaagcaggggatgtacatgttctccagcatttggaatggagacaactgggcaacgag 120 aggcggccttgagaagaccaactgggcggctgctccgttcgtatcttcgtacaagaaatt 180 ccatggcctcggctgcaaatgggaggatcaaaacacgacccaatcgtcctgtgctcacag 240 taataatgcaagtgcaaggcactggtgggataagcccgaggcgcggactctgacgaag 298 <210> 801 <211> 268 <212> DNA
<213> Pinus radiata <400> 801 tgaagctcaa gcgcgtacca ggagactctg caggagttgt cactgcttat tatatgtctt 60 ctgacacaga catgaatagg gacgagctag attttgaatt tctagggaac agaagtggac 120 agccttatgg tcttcagacg aacatctatt caaatggtgt gggtggaagg gaacagaggc 180 atatcctctg gttcgatcca acgacagagt ttcacactta ctctatcctc tggaacgcnc 240 atcagattgt gtttttcgtg gaccaagt 268 <210> 802 <211> 395 <212> DNA
<213> Pinus radiata <400> 802 cagagtttca cacttactct atcctctgga acgctcatca gattgtgttt ttcgtggacc 60 aagttccattgagagtccacaggcacactaaggctacacgccatgtgttccctcgaaagc 120 aggggatgtacatgttctccagcatttggaatggagacaactgggcaacgagaggcggcc 180 ttgagaagaccaactgggcggctgctccgttcgtatcttcgtacaagaaattccatggcc 240 tcggctgcaaatgggaggatcaaaacacgacccaatcgtcctgtgctcacagtaataatg 300 caagtgcaaggcactggtgggataagcccgaggcgcggactctgacgaagaaacagaggg 360 aatattacaggtgggtgaacagcaaatatttgact 395 <210> 803 <211> 429 <212> DNA
<213> Pinus radiata <400>

gttgggtagcagcgtagtgcagataagctgttgttaaaatggcttctttgagtatgcaga 60 actgcttcttaattctggctctttgcttctgggcatcccattgtgcacagtttaatgata 120 tcttcgagcccagctgggcgacagatcatgttatgtatgagggagagctgttgaagctta 180 agctggacaatatttccggggctggctttgcttacaagacaacatatttgtttggaaaag 240 caggggcacagattaagctcgttccaggtgactctgcaggcacagttactgctttttata 300 tgtcttctgaggggactctgcacgacgaattcgatttcgaattcttggggaatgcttcgg 360 gtgagccttacattgtgcagaccgaatatctaactccaacggcactgggcaaaagggaca 420 acgtattta <210> 804 <211> 432 <212> DNA
<213> Pinus radiata <400>

gttgggtagcagcgttgtgcagataagctgttgttaaaatggcttctttgagtatgcaga 60 actgcttcttaattctggctctttgcttctgggcatcccattgtgcacagtttaatgata 120 tcttcgagcccagctgggcgacagatcatgttatgtatgagggagagctgttgaagctta 180 agctggacaatatttccggggctggctttgcttccaagacaacatatttgtttggaaaag 240 caggggcacagattaagctcgttccaggtgactctgcaggcacagttactgctttttata 300 tgtcttctgaggggactctgcacgacgaattcgatttcgaattcttgggaaatgcttcgg 360 gtgagccttacattgtgcagacgaatatctactccaacggcactggcaaaaggggacaac 420 gtatttacctct <210> 805 <211> 438 <212> DNA
<213> Pinus radiata <400>

cgggggtcctgttcgagtgtttnaagaacagggagacagagttgggtaaagtgganagca 60 attatcattatcccaaaacccaagcaatgagggtctactccagcctttggaatgcagatg 120 attgggcaaccaggggtgggcttgtgaagaccgactggactaaagctccctttgttgcat 180 ccctccgcaatttcaatgctgccgctacttcttcttttgatgccgccgcagaggaggtgg 240 ctttggaatcgaaccaagaacagaggcagaggctccagtgggtacgaaagaactacatga 300 tctacgattattgtgcagacaccaagagattcccccagggaccgcctcccgaatgcaaat 360 aaaacctcaatcctttgaattcagagaatgaactctgaattctaccttccaaggaattct 420 gattcatttctatttaca 438 <210> 806 <211> 393 <212> DNA
<213> Pinus radiata <400> 806 accatattgtgttttctgtaagatgatatccccattagggtttacaagaacaacgaagcg 60 agaggtgttccatttccgaagaatcaaagcatggggatcttcccaanncnctgggaagcc 120 gataactgggcgacgaggggcgggctggaaaagattgactggagcaaggcgccattcgtt 180 gcctcctacaggggatttgagatcgaatcctgccagtacccgggtaaagcgagctgcgtg 240 gttaacaccagcaattggtgggaagggttgagctacagcggcctcaaaccagatcaagcg 300 aggttatacaaatgggtgaggacgaattacatgatttatgattattgtaaggacacgccc 360 aggtatccagtgctgcccacttgagtgcaacgc 393 <210> 807 <211> 259 <212> DNA
<213> Pinus radiata <400>

cacagtttgaatggatatctttcgagcccagctgggcgacagatcatgttatgtatgagg 60 gagagctgttgaagcttaagctggacaatatttccggggctggctttgcttccaagacaa 120 catatttgtttggaaaagcaggggcacagattaagctcgttccaggtgactctgcaggca 180 cagttactgctttttatatgtcttctgaggggactctgcacgacgaattcgatttcgaat 240 tcttgggaaatgcttcggg 259 <210> 808 <211> 440 <212> DNA
<213> Pinus radiata <400>

gcagtttctttgtggatgatgtgcccgcacggatattttccaacaatgagaaaagaggag 60 tcccatatcctgaaactcaacccatgggcgtatactcgtcaatatggaacgcagacgatt 120 gggctactcaagggggcctcgtcaagaccgattggagccacgcacctttcatttccacat 180 acaagaatttcagcattgatgcctgtcaatattcctcgaaaacgagctgcgtttcgtggt 240 gggatgagcctgcttacgcttctcttgatggaaagcagaggctgaaactgaaatgggtac 300 acgagaaatacatgacttacgattactgcaaagattctgtcagatttcccacgcgtccag 360 cagagtgtgaatgaaatgcccttcttaaacttgagctctcatcaagatcccccttcattt 420 ctttgtattgcatgcattcc 440 <210> 809 <211> 263 <212> DNA
<213> Pinus radiata <400> 809 gaagccatgaagaggacacattttcttgttctgtttcncattctgttactccattctgct 60 gccatggctaccactcccaggaagccagtgagtgtgccctttcacaacaattatgtcgcc 120 agctggggctcagatcacatcaaacaattccatggcggtcgaaagactgagctgctcctc 180 aacaaacagtatggtgcggggtttgantcgaaggggacatatttatttgggcatttcagt 240 atgcagataaagctggttgccgg 263 <210> 810 <211> 423 <212> DNA
<213> Pinus radiata <400> 810 aaatgacaaa taaactgttg ttaaaatggc ttctttgagt atgcagagct gcttcttaat 60 tctggtctct ttgcttctgg gcatcccatt gtgcacagtt taatgatatc ttcgagccca 120 gctgggcgac agatcatgtt atgtatgagg gagagctgtt gaagcttaag ctggacaata 180 tttccggggctggctttgcttccaagacaacatatttgtttggaaaagcaggggcacaga 240 ttaagctcgttccaggtgactctgcaggcacagttactgctttttatatgtcttctgagg 300 ggactctgcacgacgaattcgatttcgaattcttgggaaatgcttcgggtgagccttaca 360 ttgtgcagacgaatatctactccaacggcactggcaaaagggacaacgtattaacctctg 420 ggt 423 <210> 811 <211> 483 <212> DNA
<213> Pinus radiata <400>

gtcaaaagcatttctcttttggatagctagcaggaggctggtgcttttctaggcaatgct 60 gatcaatatggtgcccaatgtactgttatttctcttggtagcggcaatggctgctactgc 120 taccccacctccgaagcctgtggatgtgccattccaaaaaaactatgtacccacctgggc 180 ttctgatcatatcaagtacattaatggggggaacgaagcgcactttctcttgacaaatgg 240 acaggtactggcttccaatccaagggtagctacttgtttggacatttcagtatgcagata 300 aagatggttcctggtgactctgcaggcgttgtgactgccttttatttatcctctcagaac 360 tctgaacatgatgaaatagactttgagttcttgggcaataggtctggacaaccttacatt 420 ctccaaactaatgttttcagtggaggaaggggggcaagagagcaacgcgaatatccctgg 480 ttt 483 <210> 812 <211> 323 <212> DNA
<213> Pinus radiata <400>

tgacaatggacaagagttgcagcttactcttgaccgctcttcaggttgtggtattcaatc 60 caagcaagagtatctatttgccaaagattgatatccaaatcaagttggtacctggcaact 120 ctgcaggcacagtcactaccttttatctatcatctcaaggtcccaaacacgacgaaatag 180 acttcgaatttctgggcaacctgtccggagatccttatattttgcacactaatgtctttg 240 ctcaaggccttggtgggcgtgagcagcaattttacttgtggttcgacccaaccctggatt 300 tccacacttactccgtgctctgg 323 <210> 813 <211> 430 <212> DNA
<213> Pinus radiata <400>

tgacaatggacaagagttgcagcttactcttgaccgctcttcaggttgtggtattcaatc 60 caagcaagagtatctatttgccaagattgatatccaaatcaagttggtacctggcaactc 120 tgcaggcacagtcactaccttttatctatcatctcaaggtcccaaacacgacgaaataga 180 cttcgaatttctgggcaacctgtccggagatccttatattttgcacactaatgtctttgc 240 tcaaggccttggtgggcgtgagcagcaattttacttgtggttcgacccaaccctggattt 300 ccacacttactccgtgctctggacatcaaaccaaattatattttctgtagacgggagtcc 360 tattcgagtgttaagaacagggagacagagttgggaaagtggaaacaattatcattatcc 420 caagagcaag 430 <210> 814 <211> 331 <212> DNA
<213> Pinus radiata <400> 814 gtttcgggcg ccagtgaagg tcgttggaag ccatgaagag gacacagttt cttgttctgt 60 ttctcattctgttactccattctgctgccatggctaccactcccaggaagccagtgagtg120 tgccctttcacaacaattatgtcgccagctggggctcagatcacatcaaacaattccatg180 gcggtcgaaagactgagctgctcctcaacaaacagtatggtgcggggtttgagtcgaagg240 ggacatatttatttgggcatttcagtatgcagataaagctggttgccggtgattccgctg300 gcactgtcaccgccttttatctttcttctca 331 <210> 815 <211> 257 <212> DNA
<213> Pinus radiata <400> 815 gcatttacctctggttcgaccccaccgtagatttccattcctattcttttctgtggaacc 60 acaagcaagttgtattctttgtagacagtgttccgattagggtattccccaacaacgaga 120 ggctgggagtcccatatcctaagaaacagcccatganggnatcctcttcaatctggaatg 180 cagataactgggctactcaaggtgggcggctnangataaactggagccattctcctttna 240 tctccacttacaaaagg 257 <210> 816 <211> 216 <212> DNA
<213> Pinus radiata <400> 816 accccaccaa ggattaccat tcctatgctg tgctctggaa catgtaccaa attgcatttt 60 tggtagatga ggtaccaatc cgggtgttca agaacagcaa ggatctgggc gtgaggtacc 120 catttaacca gccgatgaag atctattcga gcctgtggaa tgctgatgac tgggccaccc 180 gagggggtct ggagaaaacc gactgggcca aggcgc 216 <210> 817 <211> 393 <212> DNA
<213> Pinus radiata <400>

gccagctggggctcagatcacatcaaacaattccatggcggtcgaaagactgagctgctc60 ctcaagaaacagtatggtgcggggtttgagtcgaaggggacatatttatttgggcatttc120 agtatgcagataaagctggttgccggtgattccgctggcactgtcaccgccttttatctt180 tcttctcagactgcagagcacgatgagatagactttgaattcttggggaacaagtcgggg240 gaaccctacattcttcagaccaatgtatttacgggcgggaagggtgagagagagcaccga300 atatacctctggttcgaccccaccaaggattaccattcctatgctgtgctctggaacatg360 taccaaattgcattttttggtagatgaggtacc <210> 818 <211> 457 <212> DNA
<213> Pinus radiata <400>

gccagctggggctcagatcacatcaaacaattccatggcggtcgaaagactgagctgctc 60 ctcaacaaacagtatggtgcggggtttgagtcgaaggggacatatttatttgggcatttc 120 agtatgcagataaagctggttgccggtgattccgctggcactgtcaccgccttttatctt 180 tcttctcagactgcagagcacgatgagatagactttgaattcttggggaacaagtcgggg 240 gaaccctacattcttcagaccaatgtatttacgggcgggaagggtgagagagagcaccga 300 atatacctctggttcgaccccaccaaggattaccattcctatgctgtgctctggaacatg 360 taccaaattgcatttttggtagatgaggtaccaatccgggtgttcaagaacagcaaggat 420 ctgggcgtgaggacccatttaaccagccgatgaagat 457 <210> 819 <211> 283 <212> DNA
<213> Pinus radiata <400> 819 cgantatacctctggttcgaccccaccaaggattaccattcctatgctgtgctctggaac 60 atgtaccaaattgcatttttggtagatgaggtaccaatccgggtgttcaagacacagcaa 120 ggatctgggcgtgaggtacccatttaaccagccgatgaagatctattcgagcctgtggaa 180 tgctgatgactgggccacccgagggggtctggagaaaaccgactgggccaaggcgccctt 240 catcgcctcctacagggaattccacgtcgatgcctgtgagget 283 <210> 820 <211> 342 <212> DNA
<213> Pinus radiata <400>

cattcctattcttttttgtggaaccacaagcaagttgtattctttgtagacagtgttccg 60 attagggtattccccaacaacgagaggctgggagtcccatatcctaagaaacagcccatg 120 agggtatcctcttcaatctggaatgcagataactgggctactcaaggtgggcggctgaag 180 ataaactggagccattctccttttatctccacttacaaaaggttcgacatcgatgcaaac 240 caatacggattaaatggagaatcgagaggggttattgagaatggaagtaagtggtgggac 300 aggccctctcattcttcccttactccattacaaaggcgatgc 342 <210> 821 <211> 316 <212> DNA
<213> Pinus radiata <400>

cgaatatacctctggttcgaccccaccaaggattaccattcctatgctgtgctctggaac 60 atgtaccaaattgcatttttggtagatgaggtaccaatccgggtgttcaagaacagcaag 120 gatctgggcgtgaggtacccatttaaccagccgatgaagatctattcgagcctgtggaat 180 gctgatgactgggccacccgagggggtctggagaaaaccgactgggccaaggcgcccttc 240 atcgcctcctacagggaattccacgtcgatgcctgtgaggcttctgctccggaatcggtg 300 tgcgctacgcaggggg 316 <210> 822 <211> 460 <212> DNA
<213> Pinus radiata <400>

tgacaatggacaagagttgcagcttactcttgaccgctcttcaggttgtggtattcaatc 60 caagcaagagtatctatttgccaagattgatatccaaatcaagttggtacctggcaactc 120 tgcaggcacagtcactaccttttatctatcatctcaaggtcccaaacacgacgaaataga 180 cttcgaatttctgggcaacctgtccggagatccttatattttgcacactaatgtctttgc 240 tcaaggccttggtgggcgtgagcagcaattttacttgtggttcgacccaaccctggattt 300 ccacacttactccgtgctctggacatcaaaccaaattatattttctgtagacgggagtcc 360 tattcgagtgtttaagaacagggagacagagttgggtaaagtggataacaattatcatta 420 tcccaagagccaagcaatgagcgtctactccagcctttgg 460 <210> 823 <211> 329 <212> DNA

<213> Pinus radiata <400> 823 cgtctcaaggccccaaacatgacgaaatagactttgaatttcttggcaacctctctgggg 60 atccttatattatgcacactaatgtcttcgctcaaggccttggcaatcgtgagcaacaat 120 tttacttgtggttcgacccaaccttggatttccacacttactccgtgctctggacatcaa 180 accaaatcatattctctgtagacgagactcccgttcgagtgtttaagaacagggagacag 240 agttgggtaaagtggatagcaattatcactatcctaagagccaagcaatgaaggtctatt 300 caagcctctggaatgcagatgattgggcg <210> 824 <211> 328 <212> DNA
<213> Pinus radiata <400> 824 ctttctctggaatcgccaccaatctgttttctttgtggacgatgtacctattcgggtgtt 60 ttccaacaatgagaaaagaggagtcccatttcctcaaacccgccctatgggcgtatactc 120 ttcaatatggaacgcagatgactgggctactcaaggtggccgcgtaaagaccgattggag 180 ccacgctccttttatttccacatacacaagtttcaacatcgatgcttgcaaatacagccc 240 tggtagttcatgtacttcgtggtgggatcagccggcgtacgcctcacttaccgcaaagca 300 gatcatgcaactcaagtgggtacacgag 328 <210> 825 <211> 352 <212> DNA
<213> Pinus radiata <400>

gcatttacctctggttcgaccccaccgcagatttccattcctattcttttctgtggaacc 60 acaagcaagttgtattctttgtagacagtgttccgattagggtattccccaacaacgaga 120 ggctgggagtcccatatcctaagaaacagcccatgagggtatcctcttcaatctggaatg 180 cagataactgggctactcaaggtgggcggctgaagataaactggagccattctcctttta 240 tctccacttacaaaaggttcgacatcgatgcaaaccaatacggattaaatggagaatcaa 300 gagggtttattgagaatggaagtaagtggtgggacaggccctctcattcttc 352 <210> 826 <211> 215 <212> DNA
<213> Pinus radiata <400> 826 gccagctggg gctcagatca catcaaacaa ttccatggcg gtcgaaagac tgagctgctc 60 ctcaacaaac agtatggtgc ggggtttgag tcgaagggga catatttatt tgggcatttc 120 agtatgcaga taaagctggt tgccggtgat tccgctggca ctgtcaccgc ctttnatctt 180 tcttctcaga ctgcagagca cgatgagatg acttt 215 <210> 827 <211> 463 <212> DNA
<213> Pinus radiata <400> 827 gttgacatgg cttgtttaag attgcagagc tgctgctttt tcgttctggt ttttttgctt 60 ctgggtatct aattgtgcag agttcaatga tatcttcgag cccagctggg cgattgatca 120 tgttatgaac gagggagagc tgttgaagct gaagcttggc cattttttct ggcgctggct 180 tttcttccaa ggccacatac ttgtttggaa aagtaggggc gcagattaaa ctcgttcccg 240 gcgactctgc gggcacagtg actgcatttt atatgtcttc tgaggggaca ttgcatgacg 300 aattcgattt cgaattcttg ggaaatgctt cgggtgagcc ttacattgtg cagactaata 360 tctactccaa cggcactggc gacagggaac aacgcattta cctctggttc gaccccaccg 420 cagatttcca ttcctattct tttctgtgga accacaagca agt 463 <210> 828 <211> 342 <212> DNA
<213> Pinus radiata <400>

caacttttctggcgctggcttttcttccaaggcaacatacttgtttggaaaagtaggggc 60 gcagattaaactcgttcccggcgactctgcgggcacagtgactgcattttatatgtcttc 120 tgaggggacattgcatgacgaattcgatttcgaattcttgggaaatgcttcgggtgagcc 180 ttacattgtgcagactaatatctactccaatggcactggcaacagggaacaacgcattta 240 cctctggttcgaccccaccgcagatttccattcctattcttttctgtggaaccacaagca 300 agttgtattctttgtagacagtgttccgattagggtattccc 342 <210> 829 <211> 447 <212> DNA
<213> Pinus radiata <400> 829 ccggtctgttgttgtgggtaataggatggccgggcaaaggaattggttcaagagaatcga 60 gtttattgttatattcgtggtttgcttaaactctgtttctgcacgcccggcatcatttgc 120 agaggattttaaagtgacgtgggcagatgaccatgtcaaaacaaggtcagataacaactc 180 catcgatctcatcctggatcagaattcaggggcaggatttgcctccaagaatcagtacat 240 gtttggacttgtaagcatgaacatcaaacttgtggcgggtgattctgcagggacagtcac 300 tgctttttatatgagctcggacaaggaggaagtgcgagatgaattggatttcgagtttct 360 ggggaacagatcaggccagccttatacagtccaaacaaatgtgtttgctctcgggaaggg 420 tggccgcgagcagagagtgaatctctg 447 <210> 830 <211> 471 <212> DNA
<213> Pinus radiata <400> 830 tgtttaagaatgcagagttgctgcttcttcattctggttttttgcttctgggtatctaat 60 tgtgcagagttcaatgatatcttcgagcccagctgggcgattgatcatgttatgaacgag 120 ggagagctgttgaagctgaagctcgacaacttttctggcgctggcttttcttccaaggca 180 acatacttgtttggaaaagtaggggcgcagattaaactcgttcccggcgactctgcgggc 240 acagtgactgcattttatatgtcttctgaggggacattgcatgacgaattcgatttcgaa 300 ttcttgggaaatgcttcgggtgagccttacattgtgcagactaatatctactccaatggc 360 actggcaacagggaacaacgcatttacctctggttcgaccccaccgcagatttccattcc 420 tattcttttctgtggaaccacaagcaagttgtattctttgtagacagtgtt 471 <210> 831 <211> 391 <212> DNA
<213> Pinus radiata <400> 831 taggatggcc gggcaaagga attggttcaa gagaatcgag tttattgtta tattcgtggt 60 ttgcttaaac tctgtttctg cacgcccggc atcatttgca gaggatttta aagtgacgtg 120 ggcagatgac catgtcaaaa caaggtcaga taacaactcc atcgatctca tcctggatca 180 gaattcaggg gcaggatttg cctccaagaa tcagtacatg tttggacttg taagcatgaa 240 catcaaactt gtggcgggtg attctgcagg gacagtcact gctttttata tgagctcgga 300 caaggaggaa gtgcgagatg aattggattt cgagtttctg gggaacagat caggccagcc 360 ttatacagtc caaacaaatg tgtttgctct c 391 <210> 832 <211> 304 <212> DNA
<213> Pinus radiata <400> 832 catcccctgctttcgagggaacacatggcgtgtagccttagtgtgcctgtggactctcaa 60 tggaacttggtccacgaaaaacacaatctgatgagcgttccagaggatagagtaagtgtg 120 aaactctgtcgttggatcgaaccagaggatatgcctctgttcccttccacccacaccatt 180 tgaatagatgttcgtctgaagaccataaggctgtccacttctgttccctagaaattcaaa 240 atctagctcgtccctattcatgtctgtgtcagaagacatataataagcagtgacaactcc 300 tgca 304 <210> 833 <211> 234 <212> DNA
<213> Pinus radiata <400> 833 acccatttaa ccagccgatg aagatctatt cgagcctgtg gaatgctgat gactgggcca 60 cccgaggggg tctggagaaa accgactggg ccaaggcgcc cttcatcgcc tcctacaggg 120 aattccacgt cgatgcctgt gaggcttctg ctccgcaatc ggtgtgcgct acgcaggggc 180 ggnggtggtg ggatcaggag gagttcagag acctggatgg gcggcaatgg cggt 234 <210> 834 <211> 375 <212> DNA
<213> Pinus radiata <400>

ggagcattcgcctttgtaatggagtaagggaagaatgagagggcctgtcccaccacttac 60 ttccatttttaataacccctcttggttctccatttaatccgtatttgtttgcatcaatgt 120 tgaagcctttgtaagtggagataaaaggagaatggctccagtttatcttcagccgcccac 180 cttgagtagcccagttatctgcattccagattgaagaggataccctcatgggctgtttct 240 taggatatgggactcccagcctctcgttgttggggaataccctaatcggaacactgtcta 300 caaagaatacaacttgcttgtggttccacagaaaagaataggaatggaaatctgcggtgg 360 ggtcgaaccagaggt 375 <210> 835 <211> 352 <212> DNA
<213> Pinus radiata <400> 835 gcagctttctcttgacaaatggacaggtactggcttccaatccaagggtagctacttgtt 60 tggacatttcagtatgcagataaagatggttcctggtgactctgcaggcgttgtgactgc 120 cttttatttatcctctcagaactctgaacatgatgaaatagactttgagttcttgggcaa 1B0 taggtctggacaaccttacattctccaaactaatgttttcagtggaggaaagggggacag 240 agagcaacgcgtatatctctggtttgaccccacaaaagactatcattcctacactgtcct 300 ttggaatatgcatcagattgtattctttgtggatgatgtccccatcagagtt 352 <210> 836 <211> 368 <212> DNA
<213> Pinus radiata <400>

ctataagtccggattcttcagtgctgctattaagctacaggcaggttatacagctggagt 60 cattgcagcactctatctctccaataaccaggagtacccaggtcaccatgacgaaataga 120 cattgagttcctggggacaacaccaggaaaaccctacaccttacagaccaatgtttacat 180 aaatggaacaggggatgggcaggttctcacaggcagggagttgaagtttcatctctggtt 240 tgacccaactgaagacttccataactacagccttctctggactccaagttatatcatctt 300 ctatgtagatgatattgctatccgaaagtacccaagaagaatttcatctacttatccatt 360 gaggccac 368 <210> 837 <211> 402 <212> DNA
<213> Pinus radiata <400>

ggagcattcgcctttgtaatggagtaagggaagaatgagagggcctgtcccaccacttac 60 ttccatttttaataacccctcttggttctccatttaatccgtatttgtttgcatcaatgt 120 tgaagcctttgtaagtggagataaaaggagaatggctccagtttatcttcagccgcccac 180 cttgagtagcccagttatctgcattccagattgaagaggataccctcatgggctgtttct 240 taggatatgggactcccagcctctcgttgttggggaataccctaatcggaacactgtcta 300 caaagaatacaacttgcttgtggttccacagaaaagaataggaatggaaatctgcggtgg 360 ggtcgaaccagaggtaaatgcgttgttccctgtcgccagtgc 402 <210> 838 <211> 389 <212> DNA
<213> Pinus radiata <400> 838 cacagactcgttgggtgcttactgaaagatagctagcaggaggctggtgcttttctagac 60 aatgctgatcaatatggtgcccaatgtactgttatttctcttggtagcggcaatggctgc 120 tactgctacctcacctccgaagcctgtggatgtgccattccaaaaaaactatgtacccac 180 ctgggcttctgatcatatcaagtacattaatggggggaacgaagcgcagctttctcttga 240 caaatggacaggtactggcttccaatccaagggtagctacttgtttggacatttcagtat 300 gcagataaagatggttcctggtgactctgcaggcgttgtgactgccttttatttatcctc 360 tcagaactctgaacatgatgaaatagact 389 <210> 839 <211> 451 <212> DNA
<213> Pinus radiata <400> 839 ggatttaggagtgaggtatccattcaaccagcccatgaaaatctattcaagcttgtggaa 60 tgctgatgactgggctacaaggggtgggttggagaagacagactggagcaaggcaccctt 120 tgttgcatcatacaagggattccacgtggatgggtgtgaggcgtctatgcctcactctgc 180 ttgtccaactttaggccgtcgatggtgggatcagaaagccttcgatgaccttgatggaca 240 gcaatggaggaaactgaagtgggttcgtgataggtacaccatatacaactactgcactga 300 cagagtgaggtatcctaaaatgtctccagagtgtaccanagaccgtgacatctaatagca 360 cagcctccttgggatagatagctatatttttattctattcttcttcgacatatggctgtt 420 ctaattatgttataactgccatttcgtagta 451 <210> 840 <211> 459 <212> DNA
<213> Pinus radiata <400>

gaataatttcaggtagtggattcaagtctttggaggcctataagtccggattcttcagtg 60 ctgctattaagctacaggcaggttatacagctggagtcattgcagcactctatctctcca 120 ataaccaggagtacccaggtcaccatgacgaaatagacattgagttcctggggacaacac 180 caggaaaaccctacaccttacagaccaatgtttacataaatggaacaggggatgggcagg 240 ttctcacaggcagggagttgaagtttcatctctggtttgacccaactgaagacttccata 300 actacagccttctctggactccaagttatatcatcttctatgtagatgatattgctatcc 360 gaaagtacccaagaagaatttcatctacttatccattgaggccactttgggtatatggat 420 caatatgggatgcttcctcttgggctactgaaaatggca 45g <210> 841 <211> 476 <212> DNA
<213> Pinus radiata <400> 841 ttttttttttgaatcatcaaaagaatctcgcttttgtattaccagtaccctaaatgaatt 60 gattgataccctctaatattttacatgtgtgtctgagaaagaatgaaagaattaatgtaa 120 tagaaatgaatcagaatacttggaaggaaagaattcagagttcattctctgaattcaaag 180 gattgaggttttatttgcattcgggaggcggtccctgggggaatctcttggtgtctgcac 240 aataatcgtagatcatgtagttctttcgtacccactgtagcctctgcctctgttcttgct 300 tcgattccaaagccacctcctctgcggcggcgtcaaaagaagaagtagcggcagcattga 360 aattgcggaaggatgcaacaaagggagctttagtccagtcggtcttcacaagcccacccc 420 tggttgcccaatcatctgcattccaaaggctggagtagaccctcattgctttgggt 476 <210> 842 <211> 293 <212> DNA
<213> Pinus radiata <400>

ggctgatcatggcagtaatcgtaagtcaaatatttgctgttcacccacctgtaatattcc 60 ctctgtttcttcgtcagagtccgcgcctcgggcttatcccaccagtgccttgcacttgca 120 ttattactgtgagcacaggacgattgggtcgtgttttgatcctcccatttgcagccgagg 180 ccatggaatttcttgtacgaagatacgaacggagcagccgcccagttggtcttctcaagg 240 ccgcctctcgttgcccagttgtctccattccaaatgctggagaacatgtacat 293 <210> 843 <211> 460 <212> DNA
<213> Pinus radiata <400> 843 gtgttgattggtctgattgcctgtgtttcttgccagtccgacggtgatgattcatctccc 60 aaggtttttgatgataattttcagatattgtgggctcaggatcacttcaggacctctgaa 120 aatggtcaagtatggcacctggttcttgaccagaactcaggttctgggttcaaatcgaag 180 aacaagtatagattcggatggttcagcatgaagctcaagctcgtaccaggagactctgca 240 ggagttgtcactgcttattatatgtcttctgacacagacatgaatagggacgagctagat 300 tttgaatttctagggaacagaagtggacagccttatggtcttcagacgaacatctattca 360 aatggtgtgggtggaagggaacagaggcatatcctctggttcgatccancgacagagttt 420 tacacttactctatcctctggaacgctcatcagattgtgt 460 <210> 844 <211> 491 <212> DNA
<213> Pinus radiata <400> 844 tggtgactctgcaggcgttgtgactgccttttatttatcctctcagaactctgaacatga 60 tgaaatagactttgagttcttgggcaataggtctggacaaccttacattctccaaactaa 120 tgttttcagtggaggaaagggggacagagagcaacgcgtatatctctggtttgaccccac 180 aaaagactatcattcctacactgtcctttggaatatgcatcagattgtattctttgtgga 240 tgatgtccccatcagagttttcaagaacagcaaggatttaggagtgaggtatccattcaa 300 ccagcccatgaaaatctattcaagcttgtggaatgctgatgactgggctacaaggggtgg 360 gttggagaagacagactggagcaaggcaccctttgttgcatcatacaagggattccacgt 420 ggatgggtgtgaggcgtctatgcctcactctgcttgtccaactttaggcccgtcgatggt 480 gggatcaagaa 491 <210> 845 <211> 413 <212> DNA
<213> Pinus radiata <400> 845 gtttgcacagactcgttgggtgcttactgaaagatagctagcaggaggctggtgcttttc 60 taggcaatgctgattaatatggtgcccaatgtactgttatttctcttggtagcggcaatg 120 gctgctactgctaccccacctccgaagcctgtggatgtgccattccaaaaaaactatgta 180 cccacctgggcttctgatcatatcaagtacattaatggggggaacgaagcgcagctttct 240 cttgacaaatggacaggtactggcttccaatccaagggtagctacttgtttggacatttc 300 agtatgcagataaagatggttcctggtgactctgcaggcgttgtgactgccttttattta 360 tcctctcagaactctgaacatgatgaaatagactttgagttcttgggcaatag 413 <210> 846 <211> 513 <212> DNA
<213> Pinus radiata <400>

gggagacagagttgggtaaagtggatagcaattatcattatcccaaaacccaagcaatga 60 gggtctactccagcctttggaatgcagatgattgggcaaccaggggtgggcttgtgaaga 120 ccgactggactaaagctccctttgttgcatccctccgcaatttcaatgctgccgctactt 180 cttcttttgatgccgtcgcagaggaggtggctttggaatcgaaccaagaacagaggcaga 240 ggctccagtgggtacgaaagaactacatgatctacgattattgtgcagacaccaagagat 300 tcccccagggaccgcctcccgattgcaaataaaacctcaatcctttgaattcagagaatg 360 aactctgaattctaccttccaagtattctgattcatttctattacattaattctcattct 420 ttctcagacacacatgtaaaatattagagggtatcaatcatttcattttggtactgctat 480 acaaaagcgagattcttttgatgaaaaaaaaaa 513 <210> 847 <211> 362 <212> DNA
<213> Pinus radiata <400> 847 ctacacctta cagaccaatg tttacataaa tggaacaggg gatgggcagg ttctcacagg 60 cagggagttg aagtttcatc tctggtttga cccaactgaa gacttccata actacagcct 120 tctctggact ccaagttata tcatcttcta tgtagatgat attgctatcc gaaagtaccc 180 aagaagaatt tcatctactt atccattgag gccactttgg gtatatggat caatatggga 240 tgcttcctct tgggctactg aaaatggcaa atacagagca gattacagat atcagccatt 300 tgttgctaag ttctctaagt tcattctcag tggctgccct gtttcagact ccacatgctc 360 ag 362 <210> 848 <211> 417 <212> DNA
<213> Pinus radiata <400>

ctcgtttctggaactgctgctctgcatcacaaactcttttcccaatccctaatcgacatc 60 gacatcgacatcgacatcgacacacctcttcacctcctctttcttttattggcctcctcg .

gcagctcttgcttctgcaaatttctacaatgatgtcgacattacatggggtaatgatcgt 180 gctaaaatcattgacaatggacaagagttgcagcttactcttgaccgctcttcaggttgt 240 ggtattcaatccaagcaagagtatctatttgccaagattgatatccaaatcaagttggta 300 cctggcaactctgcaggcacagtcactaccttttatctatcatctcaaggtcccaaacac 360 gacgaaatagacttcgaatttctgggcaacctgtccggagatccttatattttgcac 417 <210> B49 <211> 291 <212> DNA
<213> Pinus radiata <400> 849 atattatgcacactaatgtcttcgctcaaggccttggtaatcgtgagcaacaattttact 60 tgtggttcgacccaaccttggatttccacacttactccgtgctctggacatcaaaccaaa 120 tcatattctctgtagacgagactcccgttcgagtgtttaagaacagggagacagagttgg 180 gtaaagtggatagcaattatcactatcctaagagccaagcaatgaaggtctattcaagcc 240 tctggaatgcagatgattgggcgactagaggtggactcgtcaagacagact 291 <210> 850 <211> 299 <212> DNA
<213> Pinus radiata <400>

actgtcccaaggggagccgctccagctcaagctcgatcccgcttctggtgcagggtttgc 60 ttccaagcacacatacattttcggaaaagtgaatgggcaaattaaactcgttcctggaga 120 ctctgctggcaccggtattgctttctatatgtcttcccaaggggacgaacacgatgaatt 180 tgactttgaatttttgggtaacatttctggacagccatacactgtgcagaccaatgttta 240 ttcaaaaggcagtggcaatagggagcaacgcatgttcttgtggtttgacccaactgtag 299 <210> 851 <211> 359 <212> DNA
<213> Pinus radiata <400> 851 gtgttcagtagtctgattgctagtgtttgttcccagtcccaaggggatgattcatctgct 60 aaggtttttgatgataattttcagataatgtgggctgaggatcatttcaggacctctgaa 120 aatggccaagtatggcacctggttcttgaccagaactcaggttctgggttcaagtccaag 180 tataagtacagattcggatggtttagcatgaagctcaagctcgtaccgggagactctgca 240 ggagttgtcactgcttattatatgtcttctaacaccgacatgaatagggacgagctggac 300 tttgagtttctggggaacagaagtggagagccatatgctctgcagacaaacatctatgc 359 <210> 852 <211> 347 <212> DNA
<213> Pinus radiata <400> e52 gtggaattgcttgtaggaggcggtgaagggcgccttgctccaatcggttttctccagacc 60 ccctcgggtggcccagttatccgcattccacaggctcgaatagatcttcattggttggtt 120 gaaagggaaccttatccccaggttcttgcagttcttgaacacgcggattggtaccgagtc 180 cacaaaaaaactgcacaaaaaacccatcacacacatcaatattcatgaacgttccagtgc 240 caaattttgaaaagtttttttggttttttaggattttatttgtttagaattttggaagga 300 ttgtgaatttttcagaataaaaatatttaaaaaattttcaaatatct 347 <210> 853 <211> 434 <212> DNA
<213> Pinus radiata <400> 853 ggtgttcaagaacagcaaggatctgggcgtgaggtacccatttaaccagccgatgaagat 60 ctattcgagcctgtggaatgctgatgactgggccacccgagggggtctggagaaaaccga 120 ctgggccaaggcgcccttcatcgcctcctacagggaattccacgtcgatgcctgtgaggc 180 ttctgctccgcaatcggtgtgcgctacgcaggggcggcggtggtgggatcaggaggagtt 240 cagagacctggatgggcggcaatggcggtacttgaaatgggtgaggaagcactacaccat 300 ctacaattactgcactgacacgcccagaaacaagcaaatgcctccggaatgtgttcgcga 360 tcgcgacaatatgtagtccagttatcctcatcatttgatgctacccatggctgaataatc 420 cgtggtcggataat <210> 854 <211> 274 <212> DNA
<213> Pinus radiata <400>

attcaatccaagcaagagtatctatttgccaagattgatatccaaatgaagttggtacct 60 ggcaactctgcaggcacagtcactactttttatctatcatctcaaggtcccaaacacgac 120 gaaatagactttgaatttctgggaaacctgtctggagatccttatattttgcacactaat 180 gtctttgctcaaggccttggtggacgtgagcagcaattttacttgtggttcgacccaacc 240 ctggatttccacacttactccgtgctctggactt 274 <210> 855 <211> 366 <212> DNA
<213> Pinus radiata <400> 855 ataagctgttgttaaaatggcttctttgagtatgcagagctgcttcttaattctggctct 60 ttgcttctgggcatcccattgtgcacagtttaatgatatcttcgagcccagctgggcgac 120 agatcatgttatgtatgagggagagctgttgaagcttaagctggacaatatttccggggc 180 tggctttgcttccaagacaacatatttgtttggaaaagcaggggcacagattaagctcgt 240 tccaggtgactctgcaggcacagttactgctttttatatgtcttctgaggggactctgca 300 cgacgaattcgatttcgaattcttgggaaatgcttcgggtgagccttacattgggcagac 360 gaatat 366 <210> 856 <211> 398 <212> DNA
<213> Pinus radiata <400> 856 gtccagtcga atcgggtctn gaggatggca ttcgtaggtt gtcaagaagg gggtcgtgac 60 atgcactgngctcttctttgtatctttgtaacgttgtgcaacctgctcgtgagctctcaa 120 tgtgcgtncttngacgatttcttctaccccagttgggctgttgatcatgtcatgtcccaa 180 ggagagttgctccagctcaagcttgataacatttctggtgcaggatttgcttngaagagc 240 acatacatcttcggaaaagcaaatgtgcagataaagctcgttcccggggactctgctggc 300 actgttactgcattctatatgtcttcccaaggcgatcagcatgacgaattcgactttgaa 360 tttttggggaacacttttggggagccgtacgctgtgca 398 <210> 857 <211> 183 <212> DNA
<213> Pinus radiata <400> 857 cagtacatgt ttggacttgt aagcatgaac atcaaacttg tggcgggtga ttctgcaggg 60 acagtcactg ctttttatat gagctcggac aaggaggaag tgcgagatga attggatttc 120 gagtttctgg ggaacagatc aggccagcct tatacagtcc aaacaaatgt gtttgctctc 180 ggg 183 <210> 858 <211> 464 <212> DNA
<213> Pinus radiata <400> 858 cagactcgttgggtgcttactgaaagatagctagcaggaggctggtgcttttctaggcaa 60 tgctgatcaatatggtgcccaatgtactgttatttctcttggtagcggcaatggctgcta 120 ctgctaccccacctccgaagcctgtggatgtgccattccaaaaaaactatgtacccacct 180 gggcttctgatcatatcaagtacattaatggggggaacgaagcgcagctttctcttgaca 240 aatggacaggtactggcttccaatccaagggtagctacttgtttggacatttcagtatgc 300 agataaagatggntcctggtgactctgcaggcgttgtgactgccttttatttatcctctc 360 agaactctgaacatgatgaaatagactttgagttcttgggcaataggtctggacaacctt 420 acattctccaaactaatgttttcagtggaggaaagggggacaga 464 <210> 859 <211> 412 <212> DNA
<213> Pinus radiata <400> 859 tttacttgtggttcgacccaaccctggatttccacacttactccgtgctctggacttcaa 60 accaaattatattttctgtagacgggagtcctgttcgagtgtttaagaacagggagacag 120 agttgggtaaagtggatagcaattatcattatcccaaaacccaagcaatgagggtctact 180 ccagcctttggaatgcagatgattgggcaaccaggggtgggcttgtgaagaccgactgga 240 ctaaagctccctttgttgcatccctccgcaatttcaatgctgccgctacttcttcttttg 300 atgccgccgcagaggaggtggctttggaatcgaaccaagaacagaggcagaggctccagt 360 gggtacgaaagaactacatgatctacgattattgtgcagacaccaagagatt 412 <210> 860 <211> 376 <212> DNA
<213> Pinus radiata <400> 860 acgatgagat agactttgaa ttcttgggga acaagtcggg ggaaccctac attcttcaga 60 ccaatgtatt tacgggcggg aagggtgaga gagagcaccg aatatacctc tggttcgacc 120 ccaccaagga ttaccattcc tatgctgtgc tctggaacat gtaccaaatt gcatttttgg 180 tagatgaggt accaatccgg gtgttcaaga acagcaagga tctgggcgtg aggtacccat 240 ttaaccagcc gatgaagatc tattcgagcc tgtggaatgc tgatgactgg gccacccgag 300 ggggtctgga gaaaaccgac tgggccaagg cgcccttcat cgcctcctac agggaattcc 360 acgtcgatgc ctgtga 376 <210> 861 <211> 536 <212> DNA
<213> Pinus radiata <400>

gacatggcttgtttaagattgcagagctgctgctttttcgttctggttttttgcttctgg 60 gtatctaattgtgcagagttcaatgatatcttcgagcccagctgggcgattgatcatgtt 120 atgaacgagggagagctgttgaagctgaagctcgacaatttttctggcgctggcttttct 180 tccaaggccacatacttgtttggaaaagtaggggcgcagattaaactcgttcccggcgac 240 tctgcgggcacagtgactgcattttatatgtcttctgaggggacattgcatgacgaattc 300 gatttcgaattcttgggaaatgcttcgggtgagccttacattgtgcagactaatatctac 360 tccaacggcactggcgacagggaacaacgcatttacctctggttcgaccccaccgcagat 420 ttccattcctattcttttctgtggaaccacaagcaagttgtattctttgtagacagtgtt 480 ccgattagggtattccccaacaacgagaggctgggagtcccatatcctaagaaaca 536 <210> 862 <211> 358 <212> DNA
<213> Pinus radiata <400> 862 gctatatctc ccacagttta tgcagacgtc tacagtgata ttgatgtaac atggggcgaa 60 aataacgctt gggtattgaa caatggccag ggattgcagc tttctcttac caattattct 120 ggttcgggat ttcaatcgta taaggagttt ttgtttggaa gcgttgatat ctccatgaaa 180 ctggtgcccg gaaattccgc cggtaccgtt acgacatatt atctatcttc aacaggtgag 240 gggcacgacg aaattgatat ggagttccta ggaaatgttt ctggggagcc ctacattctg 300 catacaaaca tttatgtcaa tggttcagcc cgataaagag cagcagttct atttatgg 358 <210> 863 <211> 322 <212> DNA
<213> Pinus radiata <400> 863 acgatgagatagactttgaattcttggggaacaagtcgggggaaccctacattcttcaga 60 ccaatgtatttacgggcgggaagggtgagagagagcaccgaatatacctctggttcgacc 120 ccaccaaggattaccattcctatgctgtgctctggaacatgtaccaaattgcatttttgg 180 tagatgaggtaccaatccgggtgttcaagaacagcaaggatctgggcgtgaggtacccat 240 ttaaccagccgatgaagatctattcgagcctgtggaatgctgatgactgggccacccgag 300 ggggtctggagaaaaccgactg 322 <210> 864 <211> 372 <212> DNA
<213> Pinus radiata <400> 864 tccatttccgcgtgcccggctgatcaggtcttcatttttctgcaaaataatttcattttg 60 tgtaacatcgatgtgctcggaagcgatgaagactgcccaatttctgggtctgtttctcat 120 tctactactccattctgctgccatggctgccacgcccaagccagtgagtgtgcccttcgg 180 caaaaattacggtgcaagctggggctcagaccacatcaaagaattccatggaggtcgaaa 240 ggtcgaacttcttctcaacaaacagtatggtgcggggttcgaatccaaggggacatattt 300 gtttgggcat ttcagcatgc agattaagtt ggttcctggt gactcggctg gcactgtcac 360 ggccttctat ct 372 <210> 865 <211> 519 <212> DNA
<213> Pinus radiata <400> 865 ataatggcttgtttaagaatgcagagttgctgcttcttcattctggttttttgcttctgg ~

gtatctaattgtgcagagttcaatgatatcttcgagcccagctgggcgattgatcatgtt 120 atgaacgagggagagctgttgaagctgaagctcgacaacttttctggcgctggcttttct 180 tccaaggcaacatacttgtttggaaaagtaggggcgcagattaaactcgttcccggcgac 240 tctgcgggcacagtgactgcattttatatgtcttctgaggggacattgcatgacgaattc 300 gatttcgaattcttgggaaatgcttcgggtgagccttacattgtgcagactaatatctac 360 tccaacggcactggcaacagggaacaacgcatttacctctggttcgaccccaccgcagat 420 ttccattcctattcttttctgtggaaccacaagcaagttgtattctttgtagacagtgtt 480 ccgattagggtattccccaacaacgagaggctgggagtc 51g <210> 866 <211> 240 <212> DNA
<213> Pinus radiata <400> 866 ctctcatcac agggttcgaa ccgcgacgag ctggatttcg agtttctggg gaataaatct 60 ggagatccta ccattcttca aactaatgta tacgcaaatg gaaaaggcga ccgcgagcag 120 cgaatatacc tctggtttga tccatccact gaattccaca cctaccgtgt tatctggaac 180 gctgcgtata tcttatttat ggtggatgag gtgcctgtta gagtttttat gaacaacaac 240 <210> 867 <211> 392 <212> DNA
<213> Pinus radiata <400> 867 tgtttgcagagatttgttggggcaatgctgatcaagatggtccccaaactgttgctggtg 60 ctcttggtagctgcaatggctgctatggctgcctcacctcctaagcctgtggacgtacca 120 ttcccaaaaaattatgtacccacttgggctgctgatcatatcaagtacatcaatggtgga 180 aatgaggttcagctttctctagacaaatggacaggtactggcttccaatccaagggtacc 240 tacttgtttggacactttagtatgcagataaagatggttcctggtgactctgcaggcact 300 gtgactgccttttatctatcctcccagaatgccgagcacgatgaaatagattttgagttc 360 ctgggcaataggtctggacagccttacattct 392 <210> 868 <211> 605 <212> DNA
<213> Pinus radiata <400> 868 cgtgcccggctgatcaggtcttcatttttctgcaaaataatttcattttgtgtaacatcg 60 atgtgctcggaagcgatgaagactgcccaatttctgggtctgtttctcattctactactc 120 cattctgctgccatggctgccacgcccaagccagtgagtgtgcccttcggcaaaaattac 180 ggtgcaagctggggctcagaccacatcaaagaattccatggaggtcgaaaggtcgaactt 240 cttctcaacaaacagtatggtgcggggttcgaatccaaggggacatatttgtttgggcat 300 ttcagcatgcagattaagttggttcctggtgactcggctggcactgtcacggccttctat 360 ctttcttctcaaactgcagagcacgacgagatagatttcgaatttttgggcaacaggtct 420 ggacaacctt acattcttca gaccaatgta ttcacaggag gcaagggtga gagagagcat 480 cgcatatatc tctggttcga tcccaccaag gattaccatt cctatgcagt actctggaac 540 atgtaccaga tcgtgttttt tgtggactcg gtaccaatcc gcgtgttcaa gaactgcaag 600 gacct 605 <210> 869 <211> 528 <212> DNA
<213> Pinus radiata <400>

atgaattcgatttcgaattcttgggaaatgcttcgggtgagccttacattgtgcagacta 60 atatctactccaacggcactggcaacagggaacaacgcatttacctctggttcgacccca 120 cggcagatttccattcctattcttttttgtggaaccacaagcaagttgtattctttgtag 180 acagtgttccgattagggtattccccaacaacgagaggctgggagtcccatatcctaaga 240 aacagcccatgagggtatcctcttcaatctggaatgcagataactgggctactcaaggtg 300 ggcggctgaagataaactggagccattctccttttatctccacttacaaaaggttcgaca 360 tcgatgcaaaccaatacggattaaatggagaatcgagaggggttattgagaatggaagta 420 agtggtgggacaggccctctcattcttcccttactccattacaaaggcgaatgctccgat 480 gggtggatcggaactatatcatctatgactactgcaaggattcgacca 528 <210> 870 <211> 277 <212> DNA
<213> Pinus radiata <400> 870 ggaacatgtaccagatcgttttttttgtggactcggtaccaatccgcgtgttcaagaact 60 gcaagaacctggggataaggttccctttcaaccaaccaatgaagatctattcgagcctgt 120 ggaatgcggataactgggccacccgagggggtctggagaaaaccgattggagcaaggcgc 180 ccttcaccgcctcctacaagcaattccacgtcgatgcctgcgaagcttctgtttcggagt 240 cggtgtgcgctacgcaggggcggcggtggtgggatca 277 <210> 871 <211> 501 <212> DNA
<213> Pinus radiata <400>

caacaattatgtcgccagctggggctcagatcacatcaaacaattccatggcggtcgaaa 60 gactgagctgctcctcaacaaacagtatggtgcggggtttgagtcgaaggggacatattt 120 atttgggcatttcagtatgcagataaagctggttgccggtgattccgctggcactgtcac 180 cgccttttatctttcttctcagactgcagagcacgatgagatagactttgaattcttggg 240 gaacaagtcgggggaaccctacattcttcagaccaatgtatttacgggcgggaagggtga 300 gagagagcaccgaatatacctctggttcgaccccaccaaggattaccattcctatgctgt 360 gctctggaacatgtaccaaattgcatttttggtagatgaggtaccaatccgggtgttcaa 420 gaacagcaaggatctgggcgtgaggtacccatttaaccagccgatgaagatctattcgag 480 cctgtggaatgctgatgactg <210> 872 <211> 540 <212> DNA
<213> Pinus radiata <400> 872 gcgattctat tctaagcaga ccagggattc gattggagcc ttcagcatta tctgctaaat 60 ttgatcgata tcgtttgcat ctatggaggt ttctagggct ttaagttatg tccatttcct 120 tttgattgccattatctgcacagttatatctcccacagtttatgcagacgtctacagtga 180 tattgatgtaacatggggcgaaaacaacgcttgggtattgaacaatggccagggattgca 240 gctttctcttaccaattattctggttcgggatttcaatcgtataaggagtttttgtttgg 300 aagcgttgatatctccatgaaactggtgcccggaaattccgctggtaccgttacgacata 360 ctatttatcttctacaggtgaggggcacgacgaaattgatatggagtttttaggaaatgt 420 ttccgggcagccttacattctgcacacaaacatttatgtcaatggctcagccaataaaga 480 gcagcagttctatttatggttcgatccaacttcagatttccacaattactccattctctg 540 <210> 873 <211> 397 <212> DNA
<213> Pinus radiata <400>

gcccggctgatcaggtcttcatttttctgcaaaataatttcattttgtgtaacatcgatg 60 tgctcggaagcgatgaagactgcccaatttctgggtctgtttctcattctactactccat 120 tctgctgccatggctgccacgcccaagccagtgagtgtgcccttcggcaaaaattacggt 180 gcaagctggggctcagaccacatcaaagaattccatggaggtcgaaaggtcgaacttctt 240 ctcaacaaacagtatggtgcggggttcgaatccaaggggacatatttgtttgggcatttc 300 agcatgcaaattaagttggttcctggtgactcggctggcactgtcacggccttctatctt 360 tcttctcaaactgcagagcacgacgagatagatttcg 397 <210> 874 <211> 371 <212> DNA
<213> Pinus radiata <400>

ataagctgttgttaaaatggcttctttgagtatgcagaactgcttcttaattctggctct 60 ttgcttctgggcatcccattgtgcacagtttaatgatatcttcgagcccagctgggcgac 120 agatcatgttatgtatgagggagagctgttgaagcttaagctggacaatatttccggggc 180 tggctttgcttccaagacaacatatttgtttggaaaagcaggggcacagattaagctcgt 240 tccaggtgactctgcaggcacagttactgctttttatatgtcttctgaggggactctgca 300 cgacgaattcgatttcgaattcttgggaaatgcttcgggtgagccttacattgtgcaaac 360 gaatatctact 371 <210> 875 <211> 355 <212> DNA
<213> Pinus radiata <400> 875 ctttgtagacgatgtgcccgtacgggtatttgggaacaatgagaaagtgggagtcccgta 60 tcctcaatctcaaccaatgggcgtatactcttcaatatggaacgcagacgactgggctac 120 tcaaggtggcctcgtcaagaccgattggagccacgcacctttcgtttccacatacacaaa 180 tttcagcatcgatgcctgtgattattccttgaaaacgacctgctcttcgtggtgggatga 240 gcctgcttacgcttcgctcgatgcaaagcagaggctgaaactgaagtgggtgcaagagaa 300 atacatgacttacga.ttactgcaaagatttagccaggtttcccacggctcgcca 355 c <210> 876 <211> 337 <212> DNA
<213> Pinus radiata <400> 876 gcagagattt gttgggtaag ggcaatgctg atcaagatgg tccccaaact gttgctggtg 60 ctcttggtag ctgcaatggc tgctatggct gcctcacctc ctaagcctgt ggacgtacca 120 ttcccaaaaa attatgtacc cacttgggct gctgatcata tcaagtacat caatggtgga 180 aatgaggttc agctttctct agacaaatgg acaggtactg gcttccaatc caagggtacc 240 tacttgtttg gacactttag tatgcagata aagatggttc ctggtgactc tgcaggcact 300 gtgactgcct tttatctatc ctcccagaat gccgagc 337 <210> 877 <211> 558 <212> DNA
<213> Pinus radiata <400> 877 gggtagcagcgtagtgcagataagctgttgttaaaatggcttctttgagttgcagagct 60 a gcttcttaattctggctctttgcttctgggcatcccattgtgcacagtttaatgatatct 120 tcgagcccagctgggcgacagatcatgttatgtatgagggagagctgttgaagcttaagc 180 tggacaatatttccggggctggctttgcttccaagacaacatatttgtttggaaaagcag 240 gggcacagattaagctcgttccaggtgactctgcaggcacagttactgctttttatatgt 300 cttctgagggaactctgcacgacgaattcgatttcgaattcttgggaaatgcttcgggtg 360 agccttacattgttcagacgaatatctactccaacggcactggcaaaagggaacaacgta 420 tttacctctggttcgaccccacggcagatttccattcctattcttttctatggaaccaca 480 accaagttgttttctttgtggatagtgttccgattcgggtattccccaacaacgagcggc 540 tgggagtcccatatccga 558 <210> 878 <211> 400 <212> DNA
<213> Pinus radiata <400> 878 tttttttttttttttttaggaaacaccttattcgttgctgattggctttattaatatttc 60 aagcattacagagataagtcatcttgtgactgcaaaacagtaacgatgacataaataaca 120 aagcatatctaagaagaatagattacagaagatatattaatatatctataaataagagcc 180 cctaagaggctgctattaaatgtcacggtctctggtacactctggaggcattgctggaga 240 cctcactttgtcagcgcagtagttatagatggtgtaactgttacgaacccccttcagttt 300 tctccattgcattccatcaaggtcatcgaaggccttttgatcccaccaccgacgaccctg 360 agtagcacaagtcgactcagtacagaggcctcacacccat 400 <210> 879 <211> 500 <212> DNA
<213> Pinus radiata <400>

agatccctatattatgcacaccaatatcttcgctcaaggccttggcaatcgtgaacagca 60 attctacttgtggttcgacccaaccctggcttttcacacttactccgtgctctggacacc 120 aaatcaaattacattgtccgtggacgggattcctgttcgtgtgtttaaaaacagggagac 180 agaattggataaaacagatagcaattatcattatccgaagagccaagcaatgaggatgta 240 ctccagcctttggaatgcagatgattgggcaaccagaggtggcctcgtcaagacagactg 300 gactaaagccccctttgttgcatctttcagcaacttcagtgctgcgacttcttcttctaa 360 .

cttcgacggagaggatgttttggattcgaacgaagagcagaagcttcaatgggtgcgaaa 420 gaactatatgatctacgattactgtgcagacacaaaaaggttcccccaggggctacctgc 480 agaatgcaaatgagcgctct 500 <210> 880 <211> 547 <212> DNA
<213> Pinus radiata <400> 880 atgctgtgcagcctggtagtgggctctcaatgtgcagccttcaacgatcttttctacgcc 60 agtgggctgctgaccatgttatgtcccagggcgatttgctccagctcaagctcgatcaca 120 cttctggtgcaggatttgcttcgaagagcacatacttgttcggaaaagtgaatgttcaga 180 tcaagctggttcctggcgactctgctggcaccgttactgctttctatatgtcttcccaag 240 gtgatcaacatgacgaattcgactttgaattcttggggaacacttcaggagaaccgtacg 300 ttgtgcagacgaatgtttattcaaatggggttggcaacagagagcagcgtatttacctgt 360 ggttcgatccaactgcgaatttccattcctactcctttctctggaatcgccaccaatctg 420 ttttctttgtggacgatgtgcctattcgggtgttttccaacaatgagaaaagaggagtcc 480 catttcctcaaacccgccctatgggcgtatactcttcaatatggaacgcagatgactggg 540 ctactca 547 <210> 881 <211> 197 <212> DNA
<213> Pinus radiata <400> 881 tctctagaca aatggacagg tactggcttc caatccaagg gtacctactt gtttggacac 60 tttagtatgc agataaagat ggttcctggt gactctgcag gcactgtgac tgccttttat 120 ctatcctccc agaatgccga gcacgatgaa atagattttg agttcctggg caataggtct 180 ggacagcctt acattct 197 <210> 882 <211> 622 <212> DNA
<213> Pinus radiata <400> 882 catcctcctacatacattcgtttcactactctttctttgcacgcgtccatctcgagctat 60 tctattcctttcatcccttgcctacactaatctgcacttggatctcaaattcaagttcct 120 ctatttctattaacccataattatggacacaggaacgcccctcctctttcttttcttatt 180 aataacctcgtcgagtcttcttgtaattgtttctgcaaatttctacaacgatgtcgacat 240 cacatggggcgatggtcgtggtaagatccttgacaatggccaacaattacagcttactct 300 gaatcgcacttcaggttctgggtttcaatccaagaatgagtatctctttgctaaagttga 360 tatgcaaatcaagttggtacctgggaactctgccggcacagttactgcttattatctgtc 420 gtctcaaggtcccaagcacgacgaaatagactacgaatttctaggcaacctctctggaga 480 tccctatattatgcacaccaatatcttcgctcaaggccttggcaatcgtgaacagcaatt 540 ctacttgtggttcgacccaaccctggcttttcacacttactccgtgctctggacaccaaa 600 tcaaattacattgtccgtggac 622 <210> 883 <211> 223 <212> DNA
<213> Pinus radiata <400> 883 gctggtgctc ttggtagctg caatggctgc tatggctgcc tcacctccta agcctgtgga 60 cgtaccattc ccaaaaaatt atgtacccac ttgggctgct gatcatatca agtcatcaat 120 ggtggaaatg aggttcagct ttctctagac aaatggacag gtactggctt ccaatccaag 180 ggtacctact tgtttggaca ctttagtatg cagataaaga tgg 223 <210> 884 <211> 304 <212> DNA
<213> Pinus radiata <400> 884 gagagcagaggatatacctctggtttgaccccacaaaagattaccatgcctactctgtgc 60 tctggaacatgcatcagatcgtattctttgtggacgatgtccccatcagagttttcaaga 120 atagcagggacttatgagtgaggtacccatttaaccagcccatgaaaatatactcaagcc 180 tgtggaatgctgatgactgggccacaaggggtgggctggagaagacagattggagcaaag 240 caccatttgttgcatcatacaggggattccacgtggatgggtgtgaggcctctgtaactg 300 agtc 304 <210> 885 <211> 367 <212> DNA
<213> Pinus radiata <400>

aaggggtgggctggagaagacagattggagcaaagcaccatttgttgcatcatacagggg 60 attccacgtggatgggtgtgaggcctctgtaactgagtcgacttgtgctactcagggtcg 120 tcggtggtgggatcaaaaggccttcgatgaccttgatggaatgcaatggagaaaactgaa 180 gggggttcgtaacagttacaccatctataactactgcgctgacaaagtgaggtctccagc 240 aatgcctccagagtgtaccagagaccgtgacatttaatagcagcctcttaggggctctta 300 tttatagatatattaatatatcttctgtaatctattcttcttagatatgctttgttattt 360 atgtcat <210> 886 <211> 358 <212> DNA
<213> Pinus radiata <400> 886 gggccacaaggggtgggctggagaagacagattggagcaaagcaccatttgttgcatcat 60 acaggggattccacgtggatgggtgtgaggcctctgtaactgagtcgacttgtgctactc 120 agggtcgtcggtggtgggatcaaaaggccttcgatgaccttgatggaatgcaatggagaa 180 aactgaagggggttcgtaacagttacaccatctataactactgcgctgacaaagtgaggt 240 ctccagcaatgcctccagagtgtaccagagaccgtgacatttaatagcagcctcttaggg 300 gctcttatttatagatatattaatatatcttctgtaatctattcttcttagatatgct 358 <210> 887 <211> 343 <212> DNA
<213> Pinus radiata <400>

aaggggtgggctggagaagacagattggagcaaagcaccatttgttgcatcatacagggg 60 attccacgtggatgggtgtgaggcctctgtaactgagtcgacttgtgctactcagggtcg 120 tcggtggtgggatcaaaaggccttcgatgaccttgatggaatgcaatggagaaaactgaa 180 gggggttcgtaacagttacaccatctataactactgcgctgacaaagtgaggtctccagc 240 aatgcctccagagtgtaccagagaccgtgacatttaatagcagcctcttaggggctctta 300 tttatagatatattaatatatcttctgtaatctattcttctta 343 <210> 888, <211> 517 <212> DNA
<213> Pinus radiata <400> 888 ctcctacatg tttgagcgat tttactcatt cgtttcatac tctttctttg cacgcgtcca 60 tctcgagcta ttctattcct ctcatccctt gcctacacta atctgcactt ggatctcaaa 120 ttcaagttcc tctatttcta ttaatccatg attatggaca caggaacgcc cctcctcttt 180 cttttcttattaataacctcgtcgagtcttcttgtaattgtttctgcaaatttctacaac 240 gatgtcgacatcacatggggcgatcgtcgtggtaaaatccttgacaatggccaacaatta 300 cagcttactctggatcgcacttcaggttctgggtttcaatccaagaatgagtatctcttt 360 gctaaagttgatatgcaaatcaagttggtacctgggaactctgccggcacagttactgct 420 tattatctgtcgtctcaaggtcccaagcacgacgaaatagactacgaatttctaggcaac 480 ctctctggagatccctatattatgcacaccaatatct 517 <210> 889 <211> 543 <212> DNA
<213> Pinus radiata <400> 889 ctcgtgccgcttcagtttcgggcgccagtaaggtcgttggaagccatgaagaggacacag 60 tttcttgttctgtttctcattctgttactccattctgctgccatggctaccactcccagg 120 aagccagtgagtgtgccctttcacaacaattatgtcgccagctggggctcagatcacatc 180 aaacaattccatggcggtcgaaagactgagctgctcctcaacaaacagtatggtgcgggg 240 tttgagtcgaaggggacatatttatttgggcatttcagtatgcagataaagctggttgcc 300 ggtgattccgctggcactgtcaccgccttttatctttcttctcagactgcagagcacgat 360 gagatagactttgaattcttggggaacaagtcgggggaaccctacattcttcagaccaat 420 gtatttacgggcgggaagggtgagagagagcaccgaatatacctctggttcgaccccacc 480 aaggattaccattcctatgctgtgctctggaacatgtaccaaattgcatttttggtagat 540 gag 543 <210> 890 <211> 234 <212> DNA
<213> Pinus radiata <400> 890 tacatacaga ttcggatggt ttagcatgaa cctcacgctc gcaccgggac actctgcaag 60 agtggacgat gcttattata tgtcttctaa caccaacatg aatagggacg agctggactt 120 tgagtttctg gggaacagaa gtggagagcc atatgctctg cacacaaaca tctatgcaaa 180 gggtgtgggt ggtagggaac agaggcacat tctctggttc gatccaacga caca 234 <210> 891 <211> 311 <212> DNA
<213> Pinus radiata <400> 891 tgtttgcagagatttgttggggcaatgctgatcaagatggtccccaaactgttgctggtg 60 ctcttggtagctgcaatggctgctatggctgcctcacctcctaagcctgtggacgtacca 120 ttcccaaaaaattatgtacccacttgggctgctgatcatatcaagtacatcaatggtgga 180 aatgaggttcagctttctctagacaaatggacaggtactggcttccaatccaagggtacc 240 tacttgtttggacactttagtatgcagataaagatggttcctggtgactctgcaggcact 300 gggactgcctt 311 <210> 892 <211> 377 <212> DNA
<213> Pinus radiata <400> 892 caacgagagg ctgggagtcc catatcctaa gaaacagccc atgagggtat cctcttcaat 60 ctggaatgca gataactggg ctactcaagg tgggcggctg aagataaact ggagccattc 120 tccttttatc tccacttaca aaaggttcga catcgatgca aaccaatacg gattaaatgg 180 agaatcaaga gggtttattg agaatggaag taagtggtgg gacaggccct ctcattcttc 240 ccttactcca ttacaaaggc gaatgctccg atgggtgcat cggaactata tcatctatga 300 ctactgcaag gattcgacca ggttttccac ttcgccacct gagtgtgcag gcctccgctt 360 ctagttggtc tctatat 3~~
<210> 893 <211> 319 <212> DNA
<213> Pinus radiata <400> 893 tgtttgcanagatttgttggggcaatgctgatcaagatggtccccaaactgttgctggtg 60 ctcttggtagctgcaatggctgctatggctgcctcacctcctaagcctgtggacgtacca 120 ttcccaaaaaattatgtacccacttgggctgctgatcatatcaagtacatcaatggtgga 180 aatgaggttcagctttctctagacaaatggacaggtactggcttccaatccaagggtacc 240 tacttgtttggacactttagtatgcagataaagatggttcctggtgactctgcaggcact 300 gtgactgccttttatctat 319 <210> 894 <211> 342 <212> DNA
<213> Pinus radiata <400> 894 gggtagcagcgtagtgcagataagctgttgttaaaatggcttctttgagtatgcagagct 60 gcttcttaattctggctctttgcttctgggcatcccattgtgcacagtttaatgatatct 120 tcgagcccagctgggcgacagatcatgttatgtatgagggagagctgttgaagcttaagc 180 tggacaatatttccggggctggctttgcttccaagacaacatatttgtttggaaaagcag 240 gggcacagattaagctcgttccaggtgactctgcaggcacagttactgctttttatatgt 300 cttctgagggaactctgcacgacgaattcgatttcgaattct 342 <210> 895 <211> 529 <212> DNA
<213> Pinus radiata <400> 895 ctggcttccaatccaagggtacctacttgtttggacactttagtatgcagataaagatgg 60 ttcctggtgactctgcaggcactgtgactgccttttatctatcctcccagaatgccgagc 120 acgatgaaatagattttgagttcctgggcaataggtctggacagccttacattctccaaa 180 ctaatgttttcagtggaggaaaaggaaacagagagcagaggatatacctctggtttgacc 240 ccacaaaagattaccatgcctactctgtgctctggaacatgcatcagatcgtattctttg 300 tggacgatgtccccatcagagttttcaagaatagcagggacttaggagtgaggtacccat 360 ttaaccagcccatgaaaatatactcaagcctgtggaatgctgatgactgggccacaaggg 420 gtgggctggagaagacagattggagcaaagcaccatttgttgcatcatacaggggattcc 480 acgtggatgggtgtgaggcctctgtaactgagtcgacttgtgctactca 529 <210> 896 <211> 501 <212> DNA
<213> Pinus radiata <400> 896 cgcagctttc tcttgacaaa tggacaggta ctggcttcca atccaagggt agctacttgt 60 ttggacattc agtatgcaga taaagatggt tcctggtgac tctgcaggcg ttgtgactgc 120 cttttattta tcctctcaga actctgaaca tgatgaaata gactttgagt tcttgggcaa 180 taggtctgga caaccttaca ttctccaaac taatgttttc agtggaggaa agggggacag 240 agagcaacgcgtatatctctggtttgaccccacaaaagactatcattcctacactgtcct 300 ttggaatatgcatcagattgtattctttgtggatgatgtccccatcagagttttcaagaa 360 cagcaaggatttaggagtgaggtatccattcaaccagcccatgaaaatctattcaagctt 420 gtggaatgctgatgactgggctacaaggggtgggttggagaagacagactggagcaaggc 480 accctttgttgcatcatacaa 501 <210> 897 <211> 542 <212> DNA
<213> Pinus radiata <400>

aattgtttctgcaaatttctacaacgacgtcnacgtcacatggggcaatggtcgcggtaa 60 aatccttgaaatggccaacaattgcagcttactctggatcacacttcaggttctgggttt 120 caatccaagaaggagtatctgtttgctaaaattgatatgcaaatcaagttggtacctggc 180 aattctgcaggcacagttactgcctattatctgtcgtctcaaggtcccaaacacgacgaa 240 atagaccatgaatttctaggcaacctttccggagatccctatattatgcacactaatatc 300 ttcgctcaaggccttggcaatcgtgagcaacaattctacctgtggtttgacccaaccctg 360 gcttttcacacttactccgtgctctggacaccaaatcaaattacattgtctgtggacggg 420 attcccgttcgtgtgtttaagaacagggagacagagttggctaaagtggatagcaattat 480 cactgtcctaacagccaagcaatgagggtctattcaagcctctggaatgcagatgattgg 540 g~ 542 <210> 898 <211> 350 <212> DNA
<213> Pinus radiata <400> 898 tggaaatgaggttcagctttctctagacaaatggacaggtactggcttccaatccaaggg 60 tacctacttgtttggacactttagtatgcagataaagatggttcctggtgactctgcagg 120 cactgtgactgccttttatctatcctcccagaatgccgagcacgatgaaatagattttga 180 gttcctgggcaataggtctggacagccttacattctccaaactaatgttttcagtggagg 240 aaaaggaaacagagagcagaggatatacctctggtttgaccccacaaaagattaccatgc 300 ctactctgtgctctggaacatgcatcaaatcgtattctttgtggacgatg 350 <210> 899 <211> 356 <212> DNA
<213> Pinus radiata <400> 899 gtttgcacag actcgttggg tgcttactga aagatagcta gcaggaggct ggtgcttttc 60 taggcaatgc tgattaatat ggtgcccaat gtactgttat ttctcttggt agcggcaatg 120 gctgctactg ctaccccacc tccgaagcct gtggatgtgc cattccaaaa aaactatgta 180 cccacctggg cttctgatca tatcaagtac attaatgggg ggaacgaagc gcactttctc 240 ttgacaaatg gacaggtact ggcttccaat ccaagggtag ctacttgttt ggacatttca 300 atatgcagat aaagatggtt cctggtgact ctgcaagcgt tgtgactgcc ttttat 356 <210> 900 <211> 248 <212> DNA
<213> Pinus radiata <400> 900 gtgaatgtgc agattaagct cgttcctgac gactctgctg gcaccgttac tgctttctat 60 atgtcttccc aaggcgatca gcatgacgaa ttcgactttg aatttttggg gaatacttct 120 WO 00/22092 PCT/l~iZ99/00169 ggggagccgt acgctgtgca gaccaatgtt ttttctaagg gggttggcaa gcgtgagcag 180 cggattttct tatggttcga cccaaccacg gatttccatt cctattcctt tctctggaaa 240 ccgcagca 248 <210> 901 <211> 376 <212> DNA
<213> Pinus radiata <400>

caatggctgctactgctaccccacctccgaagcctgtggatgtgccattccaaaaaaact 60 atgtacccacctgggcttctgatcatatcaagtacattaatggggggaacgaagcgcagc 120 tttctcttgacaaatggacaggtactggcttccaatccaagggtagctacttgtttggac 180 atttcagtatgcagataaagatggttcctggtgactctgcaggcgttgtgactgcctttt 240 atttatcctctcagaactctgaacatgatgaaatagactttgagttcttgggcaataggt 300 ctggacaaccttacattctccaaactaatgttttcagtggaggaaagggggacagagagc 360 aacgcgtatatctctg 376 <210> 902 <211> 416 <212> DNA
<213> Pinus radiata <400>

tttgtttgcacagactcgttgggtgcttactgaaagatagctagcaggaggctggtgctt 60 ttctaggcaatgctgatcaatatggtgcccaatgtactgttatttctcttggtagcggca 120 atggctgctactgctaccccacctccgaagcctgtggatgtgccattccaaaaaaactat 180 gtacccacctgggcttctgatcatatcaagtacattaatggggggaacgaagcgcagctt 240 tctcttgacaaatggacaggtactggcttccaatccaagggtagctacttgtttggacat 300 ttcagtatgcagataaagatggttcctggtgactctgcaggcgttgtgactgccttttat 360 ttatcctctcagaactctgaacatgatgaaatagactttgagttcttgggcaatag 416 <210> 903 <211> 417 <212> DNA
<213> Pinus radiata <400>

tcgtgccgaattcggcagagctttgtcttctggggtggaagagaaggttaccaaactctc 60 ttgaatactgatctaaagaaggagcttgaccatatggccaaatttcttcaagcggccgtg 120 aactggaagaaaaccattggcttcaatgggacccttctaattgagcctaagccacaagaa 180 cctacaaaacaccagtatgattgggatgctgcaacaactatgggcttcctacaaagatat 240 gggcttggaggagaatttaaattaaatgttgagtgcaatcatgcaaccctctctgggcac 300 agctgtcatcacgagctggaaatagcacagatttatgggatgcttggaaacattgatgca 360 aacactggagatgcccaaacaggctgggatactgatcaatttcttacggatattgga 417 <210> 904 <211> 314 <212> DNA
<213> Pinus radiata <400> 904 aaacactggagatgcccagacaggttgggatactgatcaatttctcacagatattagtga 60 agcaactttgatcatgttgactgttataaaaaatggagggcttgcacctggtggtttcaa 120 ctttgatgcaaaactgaggagggaaagtgtagatgtagaagatctgttcattgctcatat 180 atcagggatggacactttggcgcgaggattgagaaatgcagctcaactgctgcaggatgg 240 aacattaacagagcttgttcgaaagcgttatgaatcctttgattcaaaattgggagcttc 300 WO 00/22092 PCTfNZ99/00169 aattgaggaa ggca 314 <210> 905 <211> 323 <212> DNA
<213> Pinus radiata <400> 905 aaacactggagatgcccagacaggttgggatactgatcaatttctcacagatattagtga 60 agcaactttgatcatgttgactgtcataaaaaatggagggcttgcacctggtggtttcaa 120 ctttgatgcaaaactgaggagggaaagtgtagatgtagaagatctgttcattgctcatat 180 atcagggatggacactttggcgcgaggattgagaaatgcagctcaactgctgcaggatgg 240 aacattaacagagcttgttcgaaagcgttatgaatcctttgattcaaaattgggagcttc 300 aattgaggaaggcagtctcaget 323 <210> 906 <211> 421 <212> DNA
<213>.Pinus radiata <400> 906 aaagatatgggcttggaggagaatttaaattaaatgttgagtgcaatcatgcaaccctct 60 ctgggcacagctgtcatcacgagctggaaatagcacagatttatgggatgcttggaaaca 120 ttgatgcaaacactggagatgcccaaacaggctgggatactgatcaatttcttacggata 180 ttggagaagcaactttaatcatgttgactgttataaagaatggtgggcttgcacctggtg 240 ggttcaactttgatgcaaaattgcggagagagagtgtagatgtggaagatctattcattg 300 ctcatatatcgggcatggacactcttgcacggggcttgagaaatgcggctaagctgttgc 360 aggatggaagaatgccagagcttgttgcaaaacgttatgaaaccttcaattcagaactag 420 g 421 <210> 907 <211> 530 <212> DNA
<213> Pinus radiata <400>

gagaggtggtgctttcatgaccgggatattgctcctgatggaagaacacttgcagaatca 60 aatgccaatctagatgctgttgttgctcttgccaaacagcttcaggaaggaactgaaatt 120 cgtcctctgtggggaactgcccagttgtttatgcatccccgctacatgcatggagctgct 180 acaagtccagatatgcatgtctatgcatatgctgcagctcaagtcaagaaagctatggag 240 gtgacaaatgaactagggggagagaattttgttttctggggtggaagagagggctatcaa 300 actttgctgaatactgatctgaaaaaggaacttgaccatatggctaggtttctacaagca 360 gcagtcaattggaagaagaatataggcttcaatggaactcttctaattgaacccaaacca 420 caagaacctacaaaacaccagtatgactgggatgctgcaacaacaatgggtttcctgcag 480 agatatggccttgcagatgaatttaaattgaacattgagtgcaaccatgc 530 <210> 908 <211> 373 < 2 ~12 > DNA
<213> Pinus radiata <400> 908 caatgggacccttctaattgagcctaagccacaagaacctacaaaacaccagtatgattg 60 ggatgctgcaacaactatgggcttcctacaaagatatgggcttggaggagaatttaaatt 120 aaatgttgagtgcaatcatgcaaccctctctgggcacagctgtcatcacgagctggaaat 180 agcacagatttatgggatgcttggaaacattgatgcaaacactggagatgcccaaacagg 240 ctgggatactgatcaatttcttacggatattggagaagcaactttaatcatgttgactgt 300 tataaagaat ggtgggcttg cacctggtgg gttcaacttt gatgcaaaat tgcggagaga 360 gagtgtagat gtg 373 CA 02345046 2001-04-05 ' .
DEMANDES OU BREVETS VOLUMINEUX
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THIS SECTION OF THE APPLfCATIONIPATENT CONTAINS MORE
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Claims (19)

Claims:
1. An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (1) sequences recited in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908; (2) complements of the sequences recited in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908;
(3) reverse complements of the sequences recited in SEQ ID NOS: 1-29. 57-80.
105, 107, 109-113, 119-129, 139-143 and 149-908; (4) reverse sequences of the sequences recited in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908; (5) nucleotide sequences producing an Expectation ("E") value of 0.01 or less when compared to a sequence recited in (1) - (4) above; (6) nucleotide sequences having at least 90% identity to a sequence of SEQ ID NO:

29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908 as determined using computer algorithm BLASTN set to default parameters; (7) nucleotide sequences having at least 75% identity to a sequence of SEQ ID NO: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908 as determined using computer algorithm BLASTN set to default parameters: (8) nucleotide sequences that hybridize to a sequence recited in (1) - (4) above under stringent hybridization conditions;
(9) nucleotide sequences that are 200-mers of a sequence recited in (1) - (4) above;
(10) nucleotide sequences that are 100-mers of a sequence recited in (1) - (4) above; (11) nucleotide sequences that are degeneratively equivalent to a sequence recited in (1) - (4) above; and (12) nucleotide sequences that are allelic variants of a sequence recited in (1) - (4) above.
2. An isolated oligonucleotide probe or primer comprising at least 20 contiguous residues complementary to 20 contiguous residues of a nucleotide sequence recited in claim 1.
3. A kit comprising a plurality of oligonucleotide probes or primers of claim 2.
4. A storage medium having recorded thereon a plurality of polynucleotides, at least one of the polynucleotides comprising a nucleotide sequence recited in claims or 2.
5. A construct comprising a polynucleotide of claim 1.
6. A transgenic cell comprising a construct according to claim 5.
7. A construct comprising, in the 5'-3' direction:

(a) a gene promoter sequence;
(b) a polynucleotide sequence comprising at least one of the following: (1) a polynucleotide coding for at least a functional portion of a polypeptide encoded by a nucleotide sequence of claim 1; and (2) a polynucleotide comprising a non-coding region of a gene coding for a polypeptide encoded by a nucleotide sequence selected from the group consisting of sequences recited in claim 1; and (c) a gene termination sequence.
8. The construct of claim 7, wherein the polynucleotide is in a sense orientation.
9. The construct of claim 7, wherein the polynucleotide is in an antisense orientation.
10. The construct of claim 7, wherein the gene promoter sequence is functional in a plant host to provide for transcription in xylem.
11. A transgenic plant cell comprising a construct of claim 7.
12. A plant comprising a transgenic plant cell according to claim 11, or a part or propagule or progeny thereof.
13. A method for modulating one or more of the polysaccharide content, the polysaccharide composition and the polysaccharide structure of a plant, comprising stably incorporating into the genome of the plant a polynucleotide of claim 1.
14. The method of claim 13 wherein the plant is selected from the group consisting of eucalyptus and pine species.
15. The method of claim 13 comprising stably incorporating into the genome of the plant a construct of claim 7.
16. A method for producing a plant having one or more of altered polysaccharide content, altered polysaccharide composition and altered polysaccharide structure, comprising:
(a) transforming a plant cell with a construct of claim 7 to provide a transgenic cell; and (b) cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
17. A method for modifying the activity of a polypeptide involved in a polysaccharide biosynthetic pathway in a plant comprising stably incorporating into the genome of the plant a construct of claim 7.
18. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: (a) sequences of SEQ ID NOS: 30-56, 81-104, 106, 108, 114-118, 129-138 and 144-148 as determined using computer algorithm BLASTP set to default parameters; (b) sequences having at least 70% identity to a sequence of SEQ ID NOS: 30, 33-36, 39, 47, 52-56, 81, 83, 84, 86, 88, 89, 91-94, 98, 99.
101, 102, 108, 114-118, 129-131, 133, 135, 138, 145 and 148 as determined using computer algorithm BLASTP set to default parameters; (c) sequences having at least 75% identity to a sequence of SEQ ID NOS: 30, 33-36, 39, 43, 45-47, 49, 56, 81, 83, 84, 86, 88, 89, 91-94, 97-99, 101, 102, 108, 114-118, 129-131, 133-135, 137, 138, 145 and 148 as determined using computer algorithm BLASTP set to default parameters; and (d) sequences having at least 90% identity to a sequence of SEQ ID NOS: 30-36, 39, 40, 42-56, 81-89, 91-104, 106, 108, 114-118, 129-135, 137, 138 and 144-148 as determined using computer algorithm BLASTP set to default parameters.
19. An isolated polypeptide encoded by an isolated polynucleotide sequence of claim 1.
CA002345046A 1998-10-13 1999-10-08 Materials and methods for the modification of plant cell wall polysaccharides Abandoned CA2345046A1 (en)

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US7049481B1 (en) 1999-05-21 2006-05-23 Board Of Control Of Michigan Technological University Cellulose synthase encoding polynucleotides and uses thereof
US7674951B1 (en) 1999-05-21 2010-03-09 Michigan Technological University Isolated cellulose synthase promoter regions
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