AU777500B2 - Materials and methods for the modification of plant cell wall polysaccharides - Google Patents

Materials and methods for the modification of plant cell wall polysaccharides Download PDF

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AU777500B2
AU777500B2 AU10837/00A AU1083700A AU777500B2 AU 777500 B2 AU777500 B2 AU 777500B2 AU 10837/00 A AU10837/00 A AU 10837/00A AU 1083700 A AU1083700 A AU 1083700A AU 777500 B2 AU777500 B2 AU 777500B2
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Leonard Nathan Bloksberg
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Description

-1- MATERIALS AND METHODS FOR THE MODIFICATION OF PLANT CELL WALL POLYSACCHARIDES Technical Field of the Invention This invention relates to the field of modification of cell wall polysaccharide content and composition in plants. More particularly, this invention relates to enzymes involved in the synthesis of plant cell wall polysaccharides and nucleotide sequences encoding such enzymes.
Background of the Invention Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field.
Plant cells are characterised by having a rigid cell wall. These cell walls are comprised primarily of polymers of simple sugar monomers linked in a variety of linear or branched polymers known as polysaccharides. The most abundant simple sugar monomer is glucose, and the most abundant polymer is cellulose. Cellulose is a linear, unbranched polymer, comprised of P-1,4 linked glucose monomers. Other polysaccharides found in plant cell walls include hemicellulose, which is a group of polysaccharides comprised of P-1,4 linked glucose monomers having side chains which may include sugars other than glucose. These side chains frequently include xylose, fucose, arabinose, and galactose. Pectins are another type of polysaccharide found in plant cell walls. Pectins are acidic polysaccharides, which are generally comprised primarily of galacturonic acid and rhamnose sugar monomers. Amylose is an additional common plant polysaccharide which is not usually found as a major component of cell walls. It acts primarily as a storage material for glucose, rather than as a structural polymer. However, because amylose is comprised primarily of a-1,4-linked glucose monomers, it is considered to be a related polymer from a biochemical and physiological perspective.
30 Plant polysaccharides have many uses. Certain plastics, such as cellulose acetate, and synthetic textiles, such as rayon, are made from cellulose. In addition, some biodegradable plastics and digestible medicine capsules, as well as medical fillers and *I fibre additives for food, can be made from plant polysaccharides.
500363762 1 DOC/BSW WO 00/22092 PCT/NZ99/00169 In foodstuffs, polysaccharides have a profound impact on food quality. Cell walls contribute to crispness in carrots, while degradation of cell walls is required for softening of fruits, such as peaches and tomatoes. In maize, increased amylose is desirable for cattle feed, but not for human consumption, and increased cell wall strength reduces digestibility. In fiber crops, such as timber, cellulose is the primary polymer of interest. Wood density, a fundamental measure of structural timber quality, is essentially a measure of cellulose content. In the paper pulping industry, efficiency is measured in terms of yield of cellulose.
Clearly, the ability to increase cellulose content in timber is an important economic goal.
The sugars which make up plant cell wall polysaccharides are produced in the photosynthetic organs of plants. The sugars so produced are commonly converted into sucrose, a disaccharide consisting of glucose and fructose. Sucrose is transported throughout the plant, to wherever sugar monomers are called for. Thus, the photosynthetic organs are often referred to as a source, while tissues requiring large amounts of sugar monomers are referred to as a sink. Actively growing regions of the plant are generally sink tissues, as new cell wall synthesis requires large amounts of sugar monomers.
When the transported sucrose arrives at the sink destination, it must be converted into whichever kind of sugar monomer is required. The sugar monomers which make up plant cell walls are primarily 5- or 6-carbon sugars. Different sugars are generally distinguished by stereospecific orientation of hydroxyl groups. Plants contain a variety of enzymes, such as isomerases or epimerases, which can rapidly change the orientation of these hydroxyls. In addition, there are a number of enzymes which can add or remove a single carbon from a sugar monomer. The result is a single pool of sugar monomers which the plant can freely inter-convert into whichever kind is needed for cell wall synthesis.
Plant polysaccharides are thus biochemically and physiologically inter-related. All polymers compete for the same pool of sugar monomers, and all sugar monomers can be fr..y intercon,vc to other types. Degradation of any one polymer will provide building material for any other. Attempts to engineer changes in one polymer may therefore have pleiotropic effects on other polymers.
The rate of cell wall synthesis is dependent on both the availability of sugar monomers to serve as building blocks for the polymers of the wall, and the enzymes which polymerise those building blocks into polymers. Enzymes which are directly responsible for the WO 00/22092 PCT/NZ99/00169 synthesis of the major cell wall polymers, such as cellulose, hemicellulose and pectin, may have a profound impact on the rate of cell wall synthesis. Source-sink relations may play an important role in limiting cell wall synthesis, if the availability of substrates becomes limiting.
Polymer degrading enzymes may liberate sugar monomers from unnecessary polymers for use in building new, desired polymers. Enzymes which can isomerise sugars from one form into another can convert the sugars into whichever kind is needed. Each of the different types of cell wall polysaccharides effectively competes for the same pool of sugar monomers, and each represents a potential source of monomers for any of the other polymers.
The final committed steps in cellulose biosynthesis involve a relatively small number of enzymes. Cellulose synthase (CEL) is believed to function as part of a large, membranebound complex which also includes sucrose synthase (SUS: Amor et al., Proc. Natl. Acad.
Sci USA 92:9353-9357, 1995) and annexin (ANX: Clark and Roux, Plant Phys. 109:1133- 1139, 1995). This enzyme complex polymerises activated glucose into the cellulose polymer.
The glucose is activated by UDP-glucose pyrophosphorylase (UGP), also known as UTPglucose-l-phosphate uridylyltransferase. These enzymes are believed to be sufficient for the biosynthesis of cellulose from glucose. Other than these steps, the availability of glucose appears to be the most significant rate-limiting step in cellulose biosynthesis.
Glucose is primarily stored in most plants as amylose. Plants routinely store amylose and degrade it to free up the glucose monomers, as needed. By inhibiting the efficiency of glucose storage, or by increasing the liberation of glucose from amylose, the availability of glucose monomers for cellulose biosynthesis can be increased. The rate-limiting enzyme in the storage of glucose as amylose is ADP-glucose pyrophosphorylase (AGP), also known as ATP-glucose-1-phosphate adenylyltransferase (Iglesias et al., J. Biol. Chem. 268:1081-1086, 1993). Conversely, the enzyme most responsible for liberating glucose from amylose is amylase (AMA: Kawagoe and Delmer, Genetic Engineering 19:63-87, 1997). The. e en-yme e'l ,nrl ;I l- These enzymes clearl will be important in the engineering of economically useful changes in cellulose biosynthesis. In addition, there are many other enzymes which may be useful in influencing plant cell wall polysaccharide biosynthesis. Other enzymes likely to be involved in cellulose biosynthesis include 1,4-p-cellobiohydrolase, P-glucosidase, calnexin, cellobiose epimerase, cellobiose phosphorylase, cellulase A, dextransucrase, invertase, phosphodiesterase, phosphoglucomutase, sucrose phosphate synthase, sucrose phosphorylase, WO 00/22092 PCT/NZ99/0I 69 UDP-glucose 4-epimerase and UDP-glucose dehydrogenase. Enzymes believed to be involved in hemicellulose biosynthesis include P-glucanase, arabinan synthase, GDP-fucose pyrophosphorylase, GDP-mannose pyrophosphorylase, 1,3 and 1,4-P-glucanases, 1,3 and 1,4f-glucosidases, mannose-6-phosphate isomerase, nDP-hexose pyrophosphorylase, xyloglucan endotransglycosylase and xyloglucan synthase. Enzymes likely to be involved in pectin biosynthesis include cc-galactosidase, P-glucuronidase, exopolygalacturonase, glucuronosyltransferase, pectin methyl-esterase, polygalacturonase and UDP-hexose- 1-phosphate uridylyltransferase. Enzymes believed to be involved in amylose biosynthesis include cx-glucosidase, amylopectin 6-glucanohydrolase, amylopectin-branching glycosyltransferase, f-amylase, branching enzyme, inulosucrase, isoamylase, isomaltase, levansucrase, starch phosphorylase and starch synthase. Enzymes likely to be involved in the interconversion of sugars include 2-dehydro-3-deoxy-gluconokinase, aldehyde reductase, arabinose isomerase, D-arabinitol dehydrogenase, D-xylulose reductase, endo-1,4-f3-xylanase, exo-l,4- P-xylanase, L-arabinose isomerase, L-ribulokinase, L-xylulokinase, phospho-ribulokinase, ribose 5-phosphate isomerase, ribulose-phosphate-3-epimerase, ribulose-phosphate-4epimerase, transaldolase, transketolase, xylose isomerase and xylulokinase. Enzymes likely to be involved in interconversion of 6-carbon sugars include 6-phospho-fructo-1-kinase, 6-phospho-fructo-2-kinase, trehalose phosphate synthase, aldolase, aldose 1-epimerase, D-fructokinase, D-galactokinase, fructose 1,6-diphosphatase, gluconolactonase, glucose -phosphatase, glucose 6-phosphatase, glucose 6-phosphate dehydrogenase, glucosephosphate isomerase, hexokinase, phosphoglucomutase, trehalase, trehalose phosphatase and UDP-galactose dehydrogenase.
While DNA sequences encoding some of the enzymes involved in the biosynthetic pathways of plant cell wall polysaccharides have been isolated for certain species of plants, genes encoding many of the enzymes in a wide range of plant species have not yet been identified. Thus, there remains a need in the art for materials useful in the modification of cell wvall polysaccharide content and composition in plants.
2. Sep. 2004 11:34 Shelston IP 11o. 1311 P. 4 Summary of the Invention Briefly, the present invention provides polynucleotides isolated from eucalyptu's and pine which encode enzymes involved in the synthesis of cell wall polysaccharides.
Genetic constructs including such sequences and methods for the use of such constructs are also provided, together with transgenic plants having altered cell wall polysaccharide content and composition.
In a first aspect, the invention provides an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: sequences recited in SEQ Mt NOS: 15-19, 71-78, 105, 139, 143 or 552-555; complements of the sequences recited in SEQ U) NOS: 15-19, 71-78, 105, 139, 143 or 552-555; reverse complements of the sequences recited in SEQ ID NOS: 15-19, 71-78, 105, 139, 143 or 552-555; reverse sequences of the sequences recited in SEQ ID NOS: 15-19, 71-78, 105, 139, 143 or 552-555; nucleotide sequences having at least 90% identity to a sequence of SEQ ID NO: 15-19, 71-78, 105, 139, 143 or 552-555; nucleotide issequences having at least 75% identity to a sequence of SEQ ED NO: 15-19, 71, 72, 74- 78, 105, 139, 143, 552, and 555; nucleotide sequences that hybridise to a sequence of SEQ ED) NO: 15-19, 71, 72, 74-78, 105, 139, 143, 552, 554 and 555 under stringent hybridisation conditions; nucleotide sequences that are 200-mers of a sequence of SEQ ID) NO: 15-19, 71-78, 105, 139, 143 and 552-555; nucleotide sequences that are 1 00-mers of a sequence of SEQ ID) NO: 15-19, 71-78, 105, 139, 143 and 552-555; Ci) nucleotide sequences that are degeneratively equivalent to a sequence of SEQ ID NO: 15-19, 71-78, 105, 139, 143 and 552-555; and nucleotide sequences that are allelie variants of a sequence of SEQ ID NO: 15-19, 71-78, 105, 139, 143 and 552-555.
In one embodiment, the isolated polynucleotides comprise a nucleotide sequence selected from the group consisting of: sequences recited in SEQ ID NOS: 15-19, 71- 78, 105, 139, 143 or 552-5 55; complements of the sequences recited in SEQ ID NOS: 15-19, 71-78, 105, 139, 143 or 552-555; reverse complements of the s~ltt~ThSEQ NOS-15:19-,7t78; ,t3i3o~25 (d)-reversesequences of the sequences recited in SEQ ID) NOS: 15-19, 71-78, 105, 139, 143 or 552- 555; and sequences having either 75% or 90% identical nucleotides 1 as defined hein to a v~i eo a-d) COMS ID No: SBMI-00896429 Received by IP Australia: Time 11.35 Date 2004-09-02 In a further aspect, isolated polypeptides encoded by a polynucleotide of the present invention are provided. In a second aspect, the invention provides an isolated oligonucleotide probe or primer comprising at least 20 contiguous residues complementary to 20 contiguous residues of a nucleotide sequence of the first aspect. In one embodiment, such polypeptides comprise an amino acid sequence selected from the group consisting of SEQ ID NOS: 44-48, 95-102, 106, 144 and 148, and variants thereof.
In another aspect, the invention provides genetic constructs comprising a polynucleotide of the present invention, either alone, in combination with one or more of the inventive polynucleotide sequences, or in combination with one or more known polynucleotides, together with transgenic cells comprising such constructs.
In a third aspect, the invention provides a kit comprising a plurality of oligonucleotide probes or primers of the second aspect.
In a fourth aspect, the invention provides a storage medium having recorded thereon a plurality of polynucleotides, at least one of the polynucleotides comprising a nucleotide sequence of the first or second aspect.
In a fifth aspect, the invention provides a genetic construct comprising a polynucleotide of the first aspect.
In a sixth aspect, the invention provides a transgenic cell comprising a construct of the fifth aspect.
In a related aspect, the present invention provides genetic constructs comprising, in the direction, a gene promoter sequence; an open reading frame coding for at least a functional portion of an enzyme encoded by a polynucleotide of the present invention or a variant thereof; and a gene termination sequence. The open reading frame S 25 may be orientated in either a sense or antisense direction. Genetic constructs comprising a non-coding region of a gene coding for an enzyme encoded by the above polynucleoiides or a nucleotide sequence complementary to a non-coding region, •together with a gene promoter sequence and a gene termination sequence, are also provided. In a seventh aspect, the invention provides a genetic construct comprising, in the direction: a gene promoter sequence; a polynucleotide sequence comprising at least one of the following: a polynucleotide coding for at least a functional portion of a polypeptide encoded by a 50036762i.Do0cBSW -7nucleotide sequence of the first aspect; and a polynucleotide comprising a noncoding region of a gene coding for a polypeptide encoded by a nucleotide sequence of the first aspect; and a gene termination sequence.
Preferably, the gene promoter and termination sequences are functional in a host plant. Most preferably, the gene promoter and termination sequences are those of the original enzyme genes but others generally used in the art, such as the Cauliflower Mosaic Virus (CMV) promoter, with or without enhancers such as the Kozak sequence or Omega enhancer, and Agrobacterium tumefaciens nopalin synthase terminator may be usefully employed in the present invention. Tissue-specific promoters may be employed in order to target expression to one or more desired tissues. In a preferred embodiment, the gene promoter sequence provides for transcription in xylem. The genetic construct may further include a marker for the identification of transformed cells.
In a further aspect, transgenic plant cells comprising the genetic constructs of the present invention are provided, together with plants comprising such transgenic cells, and fruits, seeds and other products, derivatives, or progeny of such forestry plants.
Propagules of the transgenic plants transformed with the inventive polynucleotides are also included in the present invention. As used herein, the word "propagule" means any part of a plant that may be used in reproduction or propagation, sexual or asexual, including cuttings.
In an eight aspect the invention provides a transgenic plant cell comprising a construct of the seventh aspect.
In a ninth aspect, the invention provides a transgenic plant comprising a transgenic plant cell according to the eighth aspect, or a part or propagule or progeny 25 thereof.
Plant varieties, particularly registrable plant varieties according to Plant Breeders' Rights, may be excluded from the present invention. A plant need not be -considered a "plant variety" simply because it contains stably within its genome a transgene, introduced into a cell of the plant or an ancestor thereof.
30 In yet another aspect, methods for modulating the polysaccharide content and composition of an organism, such as a plant, are provided, such methods including stably incorporating into the genome of the plant a genetic construct of the present .invention. In a tenth aspect, the invention provides a method for modulating one or 500363762 1 DOc/BSW 7amore of the polysaccharide content, the polysaccharide composition and the polysaccharide structure of a plant, comprising stably incorporating into the genome of the plant a polynucleotide of the first aspect. In a preferred embodiment, the target plant is a woody plant, preferably selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species, more preferably from the group consisting of pine and eucalyptus species, and most preferably from the group consisting of Eucalyptus grandis and Pinus radiata. In a related aspect, a method for producing a plant having modified cellulose content is provided, the method comprising transforming a plant cell with a genetic construct of the present invention to provide a transgenic cell and cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
In an eleventh aspect, the invention provides a method for producing a plant having one or more of altered polysaccharide content, altered polysaccharide composition and altered polysaccharide structure, comprising: transforming a plant cell with a construct of the seventh aspect to provide a transgenic cell; and cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
In yet a further aspect, the present invention provides methods for modifying the activity of a polypeptide in a plant, comprising stably incorporating into the genome of the plant a genetic construct of the present invention. In a twelfth aspect, the invention provides a method for modifying the activity of a polypeptide involved in a polysaccharide biosynthetic pathway in a plant comprising stably incorporating into the .genome of the plant a construct of the seventh aspect. In a preferred embodiment, the 25 target plant is a woody plant, preferably selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species, more preferably from the group consisting of pine and eucalyptus species, and most preferably from the group consisting of Eucalyptus grandis and Pinus radiata.
In a thirteenth aspect, the invention provides an isolated polypeptide comprising 30 an amino acid sequence selected from the group consisting of: SEQ ID NOS: 44-48, 95-102, 106, 144 and 148; sequences having at least 70% identity to a sequence of SEQ ID NO: 44, 46-48, 95, 96,98, 100-102, 106 and 144; 500363762_ IDOc/BSW 7b sequences having at least 75% identity to a sequence of SEQ ID NO: 44, 46-48, 95, 96, 98, 100-102, 106 and 144; and sequences having at least 90% identity to a sequence of SEQ ID NO: 44- 48, 95-102, 106, 144 and 148.
In a fourteenth aspect, the invention provides an isolated polypeptide encoded by an isolated polynucleotide sequence of the first aspect.
Unless the context clearly requires otherwise, throughout the description and the claims, the words 'comprise', 'comprising', and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to".
The abovementioned and additional features of the present invention and the manner of obtaining them will become apparent, and the invention will be best understood by reference to the following more detailed description. All references disclosed herein are hereby incorporated by reference in their entirety as if each was incorporated individually.
Brief Description of the Figures Fig. 1 illustrates the level of native CEL enzyme activity in positive control mung bean radiata) plants.
Fig. 2 illustrates the level of CEL enzyme activity in mammalian 293T cells transfected with E. grandis CEL as compared to that in non-transfected 293T cells.
Detailed Description As outlined above, cellulose is formed by polymerisation of glucose into a linear, 25 unbranched, polymer comprised of p-1,4 linked glucose monomers (Kawagoe and i: Delmer, Genetic Engineering, 19:63-87, 1997). Cellulose is the most important plant cell wall polysaccharide from both a structural, as well as industrial, perspective. Other polysaccharides are essential for healthy cell walls, as well as for many alternative industrial uses.
30 Glucose monomers are most commonly stored in the plant in the form of amylose by the action of several enzymes, with the rate limiting step for storage being catalysed by AGP (Iglesias et al., J. Biol. Chem. 268:1081-1086). Glucose monomers are freed from this storage polymer by the action of the enzyme AMA. The free 500363762 1 DOC/BSW 7c monomers are activated by the action of the enzyme UGP, and polymerised into cellulose macro-crystalline structures by the action of the cellulose synthase enzyme complex. Pure CEL enzyme has been shown to form P-1,4 glucose linkages in vitro, but has not been shown to be sufficient for polymerisation of the *fob 0 00.
4 0 60 00 *00 e v e 300363762 I.DOC/BSWt WO 00/22092 PCT/NZ99/00169 large polymers which are fundamental to the structure of plant cell walls. The holoenzyme complex appears to be necessary for this latter function. The holoenzyme is believed to be comprised of the CEL enzyme in combination with the SUS enzyme and ANX, the whole complex being integrated into the plasma membrane and forming a "rosette" structure as seen in electron micrographs of plant cell membranes (Arioli et al., Science 279:717-720, 1998).
Because cellulose synthesis can represent such a large sink for sugar monomers in the cell, changes in the rate of cellulose synthesis can have a profound influence on the synthesis of other plant polysaccharides. Conversely, changes in the rates of synthesis of other plant polysaccharides can have a profound influence on the pool of sugars available for synthesis of cellulose. Hence, changes in the synthesis of any single polymer may affect both the content and composition of plant cell wall polysaccharides, and polysaccharides in general.
Quantitative and qualitative modifications in plant polysaccharide content are known to be induced by external factors such as light stimulation, low calcium levels, and mechanical stress. Synthesis of cell wall polysaccharides can also be induced by infection with pathogens.
Using the methods and materials of the present invention, the polysaccharide content of a plant may be increased or reduced, by incorporating additional copies of genes encoding enzymes involved in the synthesis of cell wall polysaccharides into the genome of the target plant. Similarly, an increase or decrease in polysaccharide content may be obtained by transforming the target plant with antisense copies of such genes. In addition, the number of copies of genes encoding for different enzymes in the biosynthetic pathway of cell wall polysaccharides can be manipulated to modify the relative amount of each monosaccharide synthesized, thereby leading to the formation of cell walls having altered composition. The alteration of polysaccharide composition would be advantageous, for example, in tree processing for paper.
The polynucieotides of the present invention were isolated from forestry plant sources, namely from Eucalyptus grandis and Pinus radiata, but they may alternatively be synthesized using conventional synthesis techniques. Specifically, isolated polynucleotides of the present invention include polynucleotides comprising a sequence selected from the group consisting of sequences identified as SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908; complements of the sequences identified as SEQ ID NOS: 1-29, 57-80, 105, 8 WO 00/22092 PCT/NZ99/00169 107, 109-113, 119-129, 139-143 and 149-908; reverse complements of the sequences identified as SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908; at least a specified number of contiguous residues (x-mers) of any of the above-mentioned polynucleotides; extended sequences corresponding to any of the above polynucleotides; antisense sequences corresponding to any of the above polynucleotides; and variants of any of the above polynucleotides, as that term is described in this specification.
In another embodiment, the present invention provides isolated polypeptides encoded by the DNA sequences of SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908;. The predicted amino acid sequences encoded by SEQ ID NOS: 1-22, 24-28, 57-80, 105, 107, 109-113 and 119-143, based on the best available information at the time of filing this application, are provided in SEQ ID NOS: 30-56, 81-104, 106, 108, 114-118, 129-138 and 144-148, respectively. The present invention also encompasses polynucleotides that differ from the disclosed sequences but which, due to the degeneracy of the genetic code, encode a polypeptide which is the same as that encoded by a polypeptide of the present invention. Such polynucleotides are said to be "degeneratively equivalent" to a polynucleotide sequence disclosed herein.
The polynucleotides and polypeptides of the present invention were putatively identified by DNA and polypeptide similarity searches. In the attached Sequence Listing SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908 are polynucleotide sequences, and SEQ ID NOS: 30-56, 81-104, 106, 108, 114-118, 129-138 and 144-148 are polypeptide sequences. The polynucleotides and polypeptides of the present invention, have demonstrated similarity to enzymes that are known to be involved in the synthesis of cell wall polysaccharides. The putative identity of each of the inventive polynucleotides is shown below in Table 1.
TABLE 1 DNA PROTEIN IDENTITY SEQ ID NO: SEQ ID NO:_ 1 30 AGP 2 31 AGP 3 32 AGP 4 33 AMA WO 00/22092 WO 0022092PCT/NZ99/0I 69 DNA PROTEIN
IDENTITY
SEQ ID NO: SEQ ID NO: 34
AMA
6 35
AMA
7 36
GEL
8 37
GEL
9 38
GEL
39
GEL
11 40
GEL
12 41
GEL
13 4-2
GEL
14 43
GEL
44
SUS
16 45
SUS
17 46
SUS
18 47
SUS
19 48
SUS
49
UGP
21 50
UGP
22 51
UGP
23 -UGP 24 52
ANX
53
ANX
26 54
ANX
27 55
ANX
28 56
ANX
29 -ANX 57 81
AMA
58 82
AMA
59 83
AGP
84
AGP
61 85
AGP
62 86
AGP
63 87
AGP
64 88
AGP
89
AGP
66 90
GEL
67 91
GEL
68 92
E
69 93 94 71 95 u 73 97
SUS
74 98
SUS
99
SUS
76 100
SUS
77 101
SUS
78 fo' 12SUS 79 J103
UGP
104
UGP
DNA PROTEIN IDENTITY SEQ ED NO: SEQ ID NO: 105 106 SUS 107 108 GEL 109 114 ANX 110 115 ANX 111 116 ANX 112 117 ANX 113 118 ANX 119 129 GEL 120 130 GEL 121 131 GEL 122 132 GEL 123 133 GEL 124 134 GEL 125 135 GEL 126 136 GEL 127 137 GEL 128 138 GEL 139 144 SUS 140 145 a-amylase 141 146 GEL 142 147 AGP end of SEQ ID NO: 62) 143 148 SUS ofSEQ ID NO: 74) 149-185 1 ,3-p-D-Glucanase 186 1, 4-p-ellobiohydrolase 187-196 xa,a-trehalose phosphate synthase 197-204 a-glucosidase 205-250 aldolase 251 Amylopectin 6-glucanohydrolase 252-262 P-aylase 263 3-glucosidase 264-272 Branching enzyme 273-318 D-fructokinase 319-354 D-xylulose reductase 355-365 Endo-1,3-1,4-p-glucanase 366-371 Glucan exo-1 ,3--glucosidase 372-377 Glucose 6-phosphate dehydrogenase 378-381 Glucose phosphate isomerase 382-389 Isoamylase 390-393 L-ribulokinase 394-398 Mannitol- 1-phosphate 399-478 Pectin methyl-esterase 479-506 Phosphoglucomutase L a Phospho-ribuiokinase 509-521 Ribulose-phosphate-3-epimerase 522-530 Starch phosphorylase 531-551 Sucrose phosphate synthase 552-555 SUS 556-586 Transketolase 587-591 Trehalase WO 00/22092 PCT/NZ99/00169 DNA PROTEIN
IDENTITY
SEQ ID NO: SEQ ID NO: 592-620 _UDP-glucose 4-epimerase 621-902 Xylolucan endotransglvcosylase 903-908 _Xvlose isomerase The term "polynucleotide(s)," as used herein, means a single or double-stranded polymer of deoxyribonucleotide or ribonucleotide bases and includes DNA and corresponding RNA molecules, including HnRNA and mRNA molecules, both sense and anti-sense strands, and comprehends cDNA, genomic DNA and recombinant DNA, as well as wholly or partially synthesized polynucleotides. An HnRNA molecule contains introns and corresponds to a DNA molecule in a generally one-to-one manner. An mRNA molecule corresponds to an HnRNA and DNA molecule from which the introns have been excised. A polynucleotide may consist of an entire gene, or any portion thereof. Operable anti-sense polynucleotides may comprise a fragment of the corresponding polynucleotide, and the definition of "polynucleotide" therefore includes all such operable anti-sense fragments.
The term "polypeptide", as used herein, encompasses amino acid chains of any length including full length proteins, wherein amino acid residues are linked by covalent peptide bonds. Polypeptides of the present invention may be naturally purified products, or may be produced partially or wholly using recombinant techniques.
The definition of the terms "complement", "reverse complement" and "reverse sequence", as used herein, is best illustrated by the following example. For the sequence AGGACC the complement, reverse complement and reverse sequence are as follows: complement 3' TCCTGG reverse complement 3' GGTCCT reverse sequence 5' CCAGGA 3'.
As used herein, the term "variant" covers any sequence which has at least about more preferably at least about 60%, more preferably yet at least about 75% and most preferably at least about 90% identical residues (either nucleotides or amino acids) to a sequence of the present invention. The percentage of identical residues is determined by aligning the two sequences to be compared, determining the number of identical residues in 12 WO 00/22092 PCT/NZ99/00169 the aligned portion, dividing that number by the total length of the inventive, or queried, sequence and multiplying the result by 100.
Polynucleotide or polypeptide sequences may be aligned, and percentage of identical nucleotides in a specified region may be determined against another polynucleotide, using computer algorithms that are publicly available. Two exemplary algorithms for aligning and identifying the similarity of polynucleotide sequences are the BLASTN and FASTA algorithms. The similarity of polypeptide sequences may be examined using the BLASTP algorithm. Both the BLASTN and BLASTP software are available on the NCBI anonymous FTP server (ftp://ncbi.nlm.nih.gov) under /blast/executables/. The BLASTN algorithm Version 2.0.6 [Sept-16-1998], set to the default parameters described in the documentation and distributed with the algorithm, is preferred for use in the determination of variants according to the present invention. The use of the BLAST family of algorithms, including BLASTN and BLASTP, is described at NCBI's Internet website at the URL htto://www.ncbi.nlm.nih.2ov/BLAST/newblast.html and in the publication of Altschul, Stephen F, et al., "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs," Nucleic Acids Res. 25:3389-3402, 1997. The computer algorithm FASTA is available on the Internet at the ftp site ftp://ftp.virginia.edu/pub/fasta/. Version 2.04, [February 1996], set to the default parameters described in the documentation and distributed with the algorithm, is preferred for use in the determination of variants according to the present invention. The use of the FASTA algorithm is described in Pearson WR and Lipman DJ, "Improved Tools for Biological Sequence Analysis," Proc. Natl. Acad. Sci. USA 85:2444-2448, 1988; and Pearson WR, "Rapid and Sensitive Sequence Comparison with FASTP and FASTA," Methods in Enzymol. 183:63-98, 1990.
The following running parameters are preferred for determination of alignments and identities using BLASTN that contribute to the E values and percentage identity of polynucleotides of the present invention: Unix running command: blastall -p blastn -d embldb -e 10 -G 0 -E 0 -r 1 -v 30-b 30 -i queryseq -o results; and the parameters are: -p Program Name [String]; -d Database [String]; -e Expectation value [Real]; -G Cost to open a gap (zero invokes default behavior) [Integer]; -E Cost to extend a gap (zero invokes default behavior) [Integer]; -r Reward for a nucleotide match (blastn only) [Integer]; -v Number of WO 00/22092 PCT/NZ99/00169 one-line descriptions [Integer]; -b Number of alignments to show [Integer]; -i Query File [File In]; -o BLAST report Output File [File Out] Optional.
The following running parameters are preferred for determination of alignments and identities using BLASTP that contribute to the E values and percentage identity of polypeptide sequences: For BLASTP the following running parameters are preferred: blastall -p blastp d swissprotdb -e 10 -G 0 -E 0 -v 30 -b 30 -i queryseq -o results; and parameters are: -p Program Name [String]; -d Database [String]; -e Expectation value (E) [Real]; -G Cost to open a gap (zero invokes default behavior) [Integer]; -E Cost to extend a gap (zero invokes default behavior) [Integer]; -v Number of one-line descriptions (v) [Integer]; -b Number of alignments to show [Integer]; -I Query File [File In]; -o BLAST report Output File [File Out] Optional.
The "hits" to one or more database sequences by a queried sequence produced by BLASTN, BLASTP, FASTA, or a similar algorithm, align and identify similar portions of sequences. The hits are arranged in order of the degree of similarity and the length of sequence overlap. Hits to a database sequence generally represent an overlap over only a fraction of the sequence length of the queried sequence.
The BLASTN and FASTA algorithms also produce "Expect" values for alignments.
The Expect value indicates the number of hits one can "expect" to see over a certain number of contiguous sequences by chance when searching a database of a certain size. The Expect value is used as a significance threshold for determining whether the hit to a database, such as the preferred EMBL database, indicates true similarity. For example, an E value of 0.1 assigned to a hit is interpreted as meaning that in a database of the size of the EMBL database, one might expect to see 0.1 matches over the aligned portion of the sequence with a similar score simply by chance. By this criterion, the aligned and matched portions of the sequences then have a probability of 90% of being the same. For sequences having an E value of 0.01 or less over aligned and matched portions, the probability of finding a match by chance in the EMBL database is 1% or less using the BLASTN or FASTA algorithm.
According to one embodiment, "variant" polynucleotides, with reference to each of the polynucleotides of the present invention, preferably comprise sequences having the same number or fewer nucleic acids than each of the polynucleotides of the present invention and producing an E value of 0.01 or less when compared to the polynucleotide of the present 14 WO 00/22092 PCT/NZ99/00169 invention. That is, a variant polynucleotide is any sequence that has at least a 99% probability of being the same as the polynucleotide of the present invention, measured as having an E value of 0.01 or less using the BLASTN or FASTA algorithms set at the default parameters.
According to a preferred embodiment, a variant polynucleotide is a sequence having the same number or fewer nucleic acids than a polynucleotide of the present invention that has at least a 99% probability of being the same as the polynucleotide of the present invention, measured as having an E value of 0.01 or less using the BLASTN or FASTA algorithms set at the default parameters.
Alternatively, variant polynucleotide hybridize to the polynucleotide of the present invention under stringent conditions. As used herein, "stringent conditions" refers to prewashing in a solution of 6X SSC, 0.2% SDS; hybridizing at 65 0 C, 6X SSC, 0.2% SDS overnight; followed by two washes of 30 minutes each in IX SSC, 0.1% SDS at 65 0 C and two washes of 30 minutes each in 0.2X SSC, 0.1% SDS at 65 0
C.
The present invention also encompasses polynucleotides that differ from the disclosed sequences but that, as a consequence of the discrepancy of the genetic code, encode a polypeptide having similar enzymatic activity as a polypeptide encoded by a polynucleotide of the present invention. Thus, polynucleotides comprising sequences that differ from the polynucleotide sequences recited in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, or complements, reverse sequences, or reverse complements of those sequences as a result of conservative substitutions are contemplated by and encompassed within the present invention. Additionally, polynucleotides comprising sequences that differ from the polynucleotide sequences recited in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, or complements, reverse complements, or reverse sequences as a result of deletions and/or insertions totaling less than 10% of the total sequence length are also contemplated by and encompassed within the present invention. Similarly, polypeptides comprising sequences that differ from the polypeptide sequences recited in SEQ ID NOS: 30-56, 81-104, 106, 108, 114-118, 129-138 and 144-148 as a result of amino acid substitutions, insertions, nd/or deletions totaling less than 10% of the total sequence length are contemplated by an encompassed within the present invention, provided the variant polypeptide has activity in a cell wall polysaccharide synthesis pathway.
WO 00/22092 PCT/NZ99/00169 Variants of the polypeptide sequences recited in SEQ ID NOS: 30-56, 81-104, 106, 108, 114-118, 129-138 and 144-148, wherein the variant has an activity level that is different to that of the recited polypeptide are also encompassed by the present invention. In specific embodiments, variants of the inventive sucrose synthase (SUS) polypeptides are provided wherein the N-terminal serine phosphorylation site has been replaced by an acidic amino acid (such as Asp or Glu) by, for example, site directed mutagenesis. Nakai et al. have demonstrated that SUS polypeptides mutated in this manner possess increased activity compared to wild-type SUS (Nakai et al., Plant Cell Physiol. 39:1337-1341, 1998).
Polynucleotides encoding such variants of the inventive SUS polypeptides may therefore be employed in transgenic plants to increase cellulose production.
The polynucleotides of the present invention may be isolated from various libraries, or may be synthesized using techniques that are well known in the art. The polynucleotides may be synthesized, for example, using automated oligonucleotide synthesizers Beckman Oligo 1000M DNA Synthesizer) to obtain polynucleotide segments of up to 50 or more nucleic acids. A plurality of such polynucleotide segments may then be ligated using standard DNA manipulation techniques that are well known in the art of molecular biology. One conventional and exemplary polynucleotide synthesis technique involves synthesis of a single stranded polynucleotide segment having, for example, 80 nucleic acids, and hybridizing that segment to a synthesized complementary 85 nucleic acid segment to produce a 5 nucleotide overhang. The next segment may then be synthesized in a similar fashion, with a 5 nucleotide overhang on the opposite strand. The "sticky" ends ensure proper ligation when the two portions are hybridized. In this way, a complete polynucleotide of the present invention may be synthesized entirely in vitro.
Some of the polynucleotides identified as SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908 are referred to as "partial" sequences, in that they do not represent the full coding portion of a gene encoding a naturally occurring polypeptide.
The partial polynucleotide sequences disclosed herein may be employed to obtain the corresponding full length genes for various species and organisms by, for example, screening DNA expression libraries using hybridization probes based on the polynucleotides of the present invention, or using PCR amplification with primers based upon the polynucleotides of the present invention. In this way one can, using methods well known in the art, extend a WO 00/22092 PCT/NZ99/00169 polynucleotide of the present invention upstream and downstream of the corresponding mRNA, as well as identify the corresponding genomic DNA, including the promoter and enhancer regions, of the complete gene. The present invention thus comprehends isolated polynucleotides comprising a sequence identified in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, or a variant of one of the specified sequences, that encode a functional polypeptide, including full length genes. Such extended polynucleotides may have a length of from about 50 to about 4,000 nucleic acids or base pairs, and preferably have a length of less than about 4,000 nucleic acids or base pairs, more preferably yet a length of less than about 3,000 nucleic acids or base pairs, more preferably yet a length of less than about 2,000 nucleic acids or base pairs. Under some circumstances, extended polynucleotides of the present invention may have a length of less than about 1,800 nucleic acids or base pairs, preferably less than about 1,600 nucleic acids or base pairs, more preferably less than about 1,400 nucleic acids or base pairs, more preferably yet less than about 1,200 nucleic acids or base pairs, and most preferably less than about 1,000 nucleic acids or base pairs.
Polynucleotides of the present invention also comprehend polynucleotides comprising at least a specified number of contiguous residues (x-mers) of any of the polynucleotides identified as SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, complements, reverse sequences, and reverse complements of such sequences, and their variants. Similarly, polypeptides of the present invention comprehend polypeptides comprising at least a specified number of contiguous residues (x-mers) of any of the polypeptides identified as SEQ ID NOS: 30-56, 81-104, 106, 108, 114-118, 129-138 and 144-148, and their variants. As used herein, the term "x-mer," with reference to a specific value of refers to a sequence comprising at least a specified number of contiguous residues of any of the polynucleotides identified as SEQ ID NO: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, or the polypeptides identified as SEQ ID NOS: 30-56, 81-i04, i06, 108, ii4-ii8, 129-138 and 144-148. According to preferred embodiments, the value of x is preferably at least 20; more preferably, at least 40; more preferably yet, at least 60; and most preferably, at least 80. Thus, polynucleotides and polypeptides of the present invention comprise a 20-mer, a 40-mer, a 60-mer, an 80-mer, a 100-mer, a 120-mer, a 150-mer, a 180-mer, a 220-mer, a 250-mer, or a 300-mer, 400-mer, WO 00/22092 PCT/NZ99/00169 500-mer or 600-mer ofa polynucleotide or polypeptide identified as SEQ ID NOS: 1-908, and variants thereof.
Polynucleotide probes and primers complementary to and/or corresponding to SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, and variants of those sequences, are also comprehended by the present invention. Such oligonucleotide probes and primers are substantially complementary to the polynucleotide of interest. As used herein, the term "oligonucleotide" refers to a relatively short segment of a polynucleotide sequence, generally comprising between 6 and 60 nucleotides, and comprehends both probes for use in hybridization assays and primers for use in the amplification of DNA by polymerase chain reaction.
An oligonucleotide probe or primer is described as "corresponding to" a polynucleotide of the present invention, including one of the sequences set out as SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, or a variant, if the oligonucleotide probe or primer, or its complement, is contained within one of the sequences set out as SEQ ID NOS: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908, or a variant of one of the specified sequences.
Two single stranded sequences are said to be substantially complementary when the nucleotides of one strand, optimally aligned and compared, with the appropriate nucleotide insertions and/or deletions, pair with at least 80%, preferably at least 90% to 95%, and more preferably at least 98% to 100%, of the nucleotides of the other strand. Alternatively, substantial complementarity exists when a first DNA strand will selectively hybridize to a second DNA strand under stringent hybridization conditions. Stringent hybridization conditions for determining complementarity include salt conditions of less than about 1 M, more usually less than about 500 mM, and preferably less than about 200 mM. Hybridization temperatures can be as low as 5 0 C, but are generally greater than about 22 0 C, more preferably greater than about 30"C, and most preferably greater than about 3 7°C. Longer DNA fragments may require higher hybridization temperatures for specific hybridization. Since the stringency of hybridization may be affected by other factors such as probe composition, presence of organic solvents and extent of base mismatching, the combination of parameters is more important than the absolute measure of any one alone. The DNA from plants or WO 00/22092 PCT/NZ99/00169 samples or products containing plant material can be either genomic DNA or DNA derived by preparing cDNA from the RNA present in the sample.
In addition to DNA-DNA hybridization, DNA-RNA or RNA-RNA hybridization assays are also possible. In the first case, the mRNA from expressed genes would then be detected instead of genomic DNA or cDNA derived from mRNA of the sample. In the second case, RNA probes could be used. In addition, artificial analogs of DNA hybridizing specifically to target sequences could also be used.
In specific embodiments, the oligonucleotide probes and/or primers comprise at least about 6 contiguous residues, more preferably at least about 10 contiguous residues, and most preferably at least about 20 contiguous residues complementary to a polynucleotide sequence of the present invention. Probes and primers of the present invention may be from about 8 to 100 base pairs in length or, preferably from about 10 to 50 base pairs in length or, more preferably from about 15 to 40 base pairs in length. The probes can be easily selected using procedures well known in the art, taking into account DNA-DNA hybridization stringencies, annealing and melting temperatures, and potential for formation of loops and other factors, which are well known in the art. Tools and software suitable for designing probes, and especially suitable for designing PCR primers, are available on the Internet, for example, at URL http://www.horizonpress.com/pcr/. Preferred techniques for designing PCR primers are also disclosed in Dieffenbach CW and Dyksler GS, PCR primer: a laboratory manual, CSHL Press: Cold Spring Harbor, NY, 1995.
A plurality of oligonucleotide probes or primers corresponding to a polynucleotide of the present invention may be provided in a kit form. Such kits generally comprise multiple DNA or oligonucleotide probes, each probe being specific for a polynucleotide sequence. Kits of the present invention may comprise one or more probes or primers corresponding to a polynucleotide of the present invention, including a polynucleotide sequence identified in SEQ ID NOS: 1-29, 57-80, 105, 107, 109-1 13, 1 i9-i29, 139-143 and 149-908.
In one embodiment, the present invention provides genetic that include an open reading frame coding for at least a fnictional portion of a polypeptide encoded by a polynucleotide of the present invention or a variant thereof. As used herein, the "functional portion" of a polypeptide is that portion which contains the active site essential for affecting the metabolic step, the portion of the molecule that is capable of binding one or more 19 WO 00/22092 PCT/NZ99/00169 reactants or is capable of improving or regulating the rate of reaction. The functional portion can be determined by targeted mutagenesis and screening of modified protein products with protocols well known in the art. Normally, the functional portion is 10-20 amino acids, but can be shorter or longer. The active site may be made up of separate portions present on one or more polypeptide chains and will generally exhibit high substrate specificity. The term "polypeptide encoded by a polynucleotide" as used herein, includes polypeptides encoded by a nucleotide sequence which includes the partial isolated DNA sequences of the present invention.
The open reading frame may be inserted in the genetic construct in a sense or antisense orientation, such that transformation of a target plant with the genetic construct will produce a change in the amount or structure of the polypeptide compared to the wild-type plant. Transformation with a genetic construct comprising an open reading frame in a sense orientation will generally result in modified expression of the selected gene, while transformation with a genetic construct comprising an open reading frame in an antisense orientation also generally results in modified expression of the selected gene. A population of plants transformed with a genetic construct comprising an open reading frame of the present invention in either a sense or antisense orientation may be screened for increased or reduced expression of the gene in question using techniques well known to those of skill in the art, and plants having the desired phenotypes may thus be isolated.
Alternatively, expression of a gene involved in the biosynthesis of polysaccharides may be inhibited by inserting a portion of an open reading frame of the present invention, in either sense or antisense orientation, in the genetic construct. Such portions need not be fulllength but preferably comprise at least 25 and more preferably at least 50 residues of a polynucleotide of the present invention. A much longer portion or even the full length polynucleotide corresponding to the complete open reading frame may be employed. The portion of the open reading frame does not need to be precisely the same as the endogenous sequence, provided that there is sufficient sequence similarity to achieve inhibition of the target gene. Thus a sequence derived from one species may bc used to inhibit expression of a gene in a different species.
In a second embodiment, the inventive genetic constructs comprise a polynucleotide including a non-coding region of a gene coding for a polypeptide encoded by a polynucleotide WO 00/22092 PCT/NZ99/00169 of the present invention, or a polynucleotide sequence complementary to such a non-coding region. Examples of non-coding regions which may be usefully employed in such constructs include introns and 5'-non-coding leader sequences. Transformation of a target plant with such a genetic construct may lead to a reduction in the amount of polysaccharide synthesized by the plant by the process of co-suppression, in a manner similar to that discussed, for example, by Napoli et al. (Plant Cell 2:279-290, 1990) and de Carvalho Niebel et al. (Plant Cell 7:347-358, 1995).
Alternatively, regulation of polysaccharide synthesis can be achieved by inserting appropriate sequences or subsequences DNA or RNA) in ribozyme constructs (McIntyre CL, Manners JM, Transgenic Res. 5(4):257-262, 1996). Ribozymes are synthetic RNA molecules that comprise a hybridizing region complementary to two regions, each of which comprises at least 5 contiguous nucleotides in a mRNA molecule encoded by one of the inventive polynucleotides. Ribozymes possess highly specific endonuclease activity, which autocatalytically cleaves the mRNA.
The genetic constructs of the present invention further comprise a gene promoter sequence and a gene termination sequence, operably linked to the DNA sequence to be transcribed, which control expression of the gene. The gene promoter sequence is generally positioned at the 5' end of the DNA sequence to be transcribed, and is employed to initiate transcription of the DNA sequence. Gene promoter sequences are generally found in the non-coding region of a gene but they may exist downstream of the open reading frame, in introns (Luehrsen KR, Mol. Gen. Genet. 225:81-93, 1991) or in the coding region, as for example in a plant defence gene (Douglas et al., EMBO J. 10:1767-1775, 1991). When the construct includes an open reading frame in a sense orientation, the gene promoter sequence also initiates translation of the open reading frame. For DNA constructs comprising either an open reading frame in an antisense orientation or a non-coding region, the gene promoter sequence consists only of a transcription initiation site having a RNA polymerase binding site.
A variety of gene promoter sequences which may be usefully employed in the DNA constructs of the presnt invcntion are well known in the art. The gcne promotcr scqucnce, and also the gene termination sequence, may be endogenous to the target plant host or may be exogenous, provided the promoter is functional in the target host. For example, the promoter and termination sequences may be from other plant species, plant viruses, bacterial plasmids, 21 WO 00/22092 PCT/NZ99/00169 and the like. Preferably, gene promoter and termination sequences are from the inventive sequences themselves.
Factors influencing the choice of promoter include the desired tissue specificity of the construct, and the timing of transcription and translation. For example, constitutive promoters, such as the 35S Cauliflower Mosaic Virus (CaMV 35S) promoter, will affect the activity of the enzyme in all parts of the plant. Use of a tissue specific promoter will result in production of the desired sense or antisense RNA only in the tissue of interest. With genetic constructs employing inducible gene promoter sequences, the rate of RNA polymerase binding and initiation can be modulated by external stimuli, such as light, heat, anaerobic stress, alteration in nutrient conditions and the like. Temporally regulated promoters can be employed to effect modulation of the rate of RNA polymerase binding and initiation at a specific time during development of a transformed cell. Preferably, the original promoters from the enzyme gene in question, or promoters from a specific tissue-targeted gene in the organism to be transformed, such as eucalyptus or pine are used. Other examples of gene promoters which may be usefully employed in the present invention include mannopine synthase (mas), octopine synthase (ocs), and those reviewed by Chua et al. (Science 244:174-181, 1989).
The gene termination sequence, which is located 3' to the DNA sequence to be transcribed, may come from the same gene as the gene promoter sequence or may be from a different gene. Many gene termination sequences known in the art may be usefully employed in the present invention, such as the 3' end of the Agrobacterium tumefaciens nopaline synthase gene. However, preferred gene terminator sequences are those from the original enzyme gene or from the target species to be transformed.
The genetic constructs of the present invention may also contain a selection marker that is effective in plant cells, to allow for the detection of transformed cells containing the inventive construct. Such markers, which are well known in the art, typically confer resistance to one or more toxins. One example of such a marker is the NPTII gene whose expression results in resistance to kanamycin or hygromycin, antibiotics which arc usually toxic to plant cells at a moderate concentration (Rogers et al., in Weissbach, A and H, eds., Methods for Plant Molecular Biology, Academic Press Inc.: San Diego, CA, 1988).
Transformed cells can thus be identified by their ability to grow in media containing the 22 WO 00/22092 PCT/NZ99/00169 antibiotic in question. Alternatively, the presence of the desired construct in transformed cells can be determined by means of other techniques well known in the art, such as Southern and Western blots.
A transcription initiation site is additionally included in the genetic construct when the sequence to be transcribed lacks such a site.
Techniques for operatively linking the components of the inventive genetic constructs are well known in the art and include the use of synthetic linkers containing one or more restriction endonuclease sites as described, for example, by Sambrook et al. (Molecular cloning. a laboratory manual, CSHL Press: Cold Spring Harbor, NY, 1989). The genetic construct of the present invention may be linked to a vector having at least one replication system, for example E. coli, whereby after each manipulation, the resulting construct can be cloned and sequenced and the correctness of the manipulation determined.
The genetic constructs of the present invention may be used to transform a variety of plants, both monocotyledonous grasses, corn, grains, oat, wheat and barley), dicotyledonous Arabidopsis, tobacco, legumes, alfalfa, oaks, eucalyptus, maple), and Gymnosperms Scots pine; see Aronen, Finnish Forest Res. Papers, Vol. 595, 1996), white spruce (Ellis et al., Biotechnology 11:84-89, 1993), and larch (Huang et al., In Vitro Cell 27:201-207, 1991). In a preferred embodiment, the inventive genetic constructs are employed to transform woody plants, herein defined as a tree or shrub whose stem lives for a number of years and increases in diameter each year by the addition of woody tissue.
Preferably the target plant is selected from the group consisting of eucalyptus and pine species, most preferably from the group consisting of Eucalyptus grandis and Pinus radiata.
Other species which may be usefully transformed with the DNA constructs of the present invention include, but are not limited to: pines such as Pinus banksiana, Pinus brutia. Pinus caribaea, Pinus clausa, Pinus contorta, Pinus coulteri, Pinus echinata, Pinus eldarica, Pinus ellioti, Pinus jefreyi, Pinus lambertiana, Pinus monticoia, Pinus nigra, Pinus palustrus, Pinus pinaster, Pinus ponderosa, Pinus resinosa, Pinus rigida, Pinus serotina, Pinus strobus, PiMns syvestris, Pinus taeda, Pinus virginiana; other gymnosperms, such as Abies anabilis, Abies balsamea, Abies concolor, Abies grandis, Abies lasiocarpa, Abies magnifica, Abies procera, Chamaecyparis lawsoniona, Chamaec)paris nootkatensis, Chamaecvparis thyoides.
Huniperus virginiana, Larix decidua, Larix laricina, Larix leptolepis, Larix occidentalis, WO 00/22092 PCTINZ99/00169 Larix siberica, Libocedrus decurrens, Picea abies, Picea engelmnanni, Picea glauca, Picea mlariana, Picea pungens, Picea rubens, Picea sirchensis, Pseudorsuga menziesii, Sequoia gigantea, Sequoia senhpervirens, Taxodium distichumnt, Tsuga canadensis, Tsuga heterophylla, Tsuga mertensiana, Thuja occidentalis, Thuja plicata; and Eucalypts, such as Eucalyptus alba, Eucalyptus bancroftii, Eucalyptus botyroides, Eucalyptus bridgesiana, Eucalyptus calophylla, Eucalyptus camnaldulensis Eucalyptus citriodora, Eucalyptus cladocalyx, Eucalyptus coccifera, Eucalyptus curuisii, Eucalyptus daliympleana, Eucaliptus deglupta, Eucalyptus delagatensis, Eucalyptus diversicolor, Eucalyptus dunnii, Eucalvptus ficifolia, Eucalyptus globulus, Eucalyptus gomphocephala, Eucalyptus gunnii, Eucalyptus henryi, Eucalyptus laevopinea, Eucalyptus mnacarthurii, Eucalyptus macrorhyncha, Eucalyptus maculata, Eucaljplus marginata, Eucalyptus inegacalipa, Eucalyptus melliodora, Eucalyptus nicholii, Eucalyptus nitens, Eucalyptus nova-anglica, Eucalyptus obliqua, Eucalyptus obtusiflora, Eucalyptus oreades, Eucalyptus pauciflora, Eucalyptus polybractea, Eucalyptus regnans, Eucalyptus resinifera, Eucalyptus robusta, Eucalyptus rudis, Eucalyptus saligna, Eucalyptus sideroxylon, Eucalyptus stuartiana, Eucalyptus tereticornis, Eucalyptus torelliana Eucalyptus urnigera, Eucalyptus urophylla, Eucalyptus viminalis, Eucalyptus viridis, Eucalyptus wandoo and Eucalyptus youmanni; together with hybrids of the above species.
As discussed above, transformation of a plant with a genetic construct of the present invention will result in a modification in polysaccharide synthesis in the plant. For example, an increase in the production of cellulose in a plant may be obtained by introducing a genetic construct comprising an open reading frame encoding the enzyme CEL in a sense orientation.
Similarly, transformation of a plant with a genetic construct comprising either an open reading frame encoding CEL in an antisense orientation or a non-coding (untranslated) region of a CEL gene will lead to a reduction in the cellulose content of the transformed plant.
Techniques for stably incorporating genetic constructs into the genome of target piants are well known in the art and include Agrobacterium ruiefaciens mediated introduction, electroporation, protoplast fusion, injection into reproductive organs, injection into immature embryos, high velocity projectile introduction and the like. The choice of technique will depend upon the target plant to be transformed. For example, dicotyledonous plants and certain monocots and gymnosperms may be transformed by Agrobacerium Ti plasmid WO 00/22092 PCT/NZ99/00169 technology, as described, for example by Bevan (Nucleic Acids Res. 12:8711-8721, 1984).
Targets for the introduction of the genetic constructs of the present invention include tissues, such as leaf tissue, disseminated cells, protoplasts, seeds, embryos, meristematic regions; cotyledons, hypocotyls, and the like. The preferred method for transforming eucalyptus and pine is a biolistic method using pollen (see, for example, Aronen, Finnish Forest Res. Papers, 595:53, 1996) or easily regenerable embryonic tissues.
Once the cells are transformed, cells having the inventive genetic construct incorporated in their genome may be selected by means of a marker, such as the kanamycin resistance marker discussed above. Transgenic cells may then be cultured in an appropriate medium to regenerate whole plants, using techniques well known in the art. In the case of protoplasts, the cell wall is allowed to reform under appropriate osmotic conditions. In the case of seeds or embryos, an appropriate germination or callus initiation medium is employed.
For explants, an appropriate regeneration medium is used. Regeneration of plants is well established for many species. For a review of regeneration of forest trees, see Dunstan et al., "Somatic embryogenesis in woody plants," in Thorpe TA, ed., In vitro embryogenesis of plants, (Current Plant Science and Biotechnology in Agriculture), Vol. 20, Chapter 12, pp. 471-540, 1995. Specific protocols for the regeneration of spruce are discussed by Roberts et al., ("Somatic embryogenesis of spruce," in Redenbaugh K, ed., Synseed: applications of synthetic seed to crop improvement, Chapter 23, pp. 427-449, CRC Press: 1993). The resulting transformed plants may be reproduced sexually or asexually, using methods well known in the art, to give successive generations of transgenic plants.
As discussed above, the production of RNA in target plant cells can be controlled by choice of the promoter sequence, or by selecting the number of functional copies or the site of integration of the polynucleotides incorporated into the genome of the target plant host. A target plant may be transformed with more than one genetic construct of the present invention, thereby modulating the activity of more than one cell wall polysaccharide enzyme, affecting enzyme activity in more than one tissue, or affecting enzyme activity at more than one expression time. Similarly, a genetic construct may be assembled containing more than one open reading frame coding for a polypeptide encoded by a polynucleotide of the present invention or more than one non-coding region of a gene coding for such a polypeptide. The polynucleotides of the present inventive may also be employed in combination with other WO 00/22092 PCT/NZ99/00169 known sequences encoding polypeptides involved in the synthesis of cell wall polysaccharides. In this manner, it may be possible to modify a biosynthetic pathway of cell wall polysaccharides in a non-woody plant to produce a new type of woody plant.
The following examples are offered by way of illustration and not by way of limitation.
Example 1 Isolation and Characterization of cDNA Clones from Eucalyptus grandis Eucalyptus grandis cDNA expression libraries (from various tissues, including flowers, leaves, phloem, roots, seeds, shoot buds and xylem) were constructed and screened as follows.
mRNA was extracted from the plant tissue using the protocol of Chang et al. (Plant Molecular Biology Reporter 11:113-116, 1993) with minor modifications. Specifically, samples were dissolved in CPC-RNAXB (100 mM Tris-C1, pH 8,0; 25 mM EDTA; 2.0 M NaCI; 2%CTAB; 2% PVP and 0.05% Spermidine*3HC1) and extracted with chloroform:isoamyl alcohol, 24:1. mRNA was precipitated with ethanol and the total RNA preparate was purified using a Poly(A) Quik mRNA Isolation Kit (Stratagene, La Jolla, CA).
A cDNA expression library was constructed from the purified mRNA by reverse transcriptase synthesis followed by insertion of the resulting cDNA clones in Lambda ZAP using a ZAP Express cDNA Synthesis Kit (Stratagene), according to the manufacturer's protocol. The resulting cDNAs were packaged using a Gigapack II Packaging Extract (Stratagene) employing 1 p l of sample DNA from the 5 p.l ligation mix. Mass excision of the library was done using XL1-Blue MRF' cells and XLOLR cells (Stratagene) with ExAssist helper phage (Stratagene). The excised phagemids were diluted with NZY broth (Gibco BRL, Gaithersburg, MD) and plated out onto LB-kanamycin agar plates containing X-gal and isopropylthio-beta-galactoside (IPTG).
Of the colonies plated and picked for DNA miniprep, 99% contained an insert suitable for sequencing. Positive colonies were cultured in NZY broth with kanamycin and cDNA was purified by means of alkaline lysis and polyethylene glycol (PEG) precipitation. Agarose gel at 1% was used to screen sequencing templates for chromosomal contamination. Dye primer WO 00/22092 PCT/NZ99/00169 sequences were prepared using a Turbo Catalyst 800 machine (Perkin Elmer/Applied Biosystems Division, Foster City, CA) according to the manufacturer's protocol.
DNA sequences for positive clones were obtained using a Perkin Elmer/Applied Biosystems Division Prism 377 sequencer. cDNA clones were sequenced first from the end and, in some cases, also from the 3' end. For some clones, internal sequence was obtained using subcloned fragments. Subcloning was performed using standard procedures of restriction mapping and subcloning to pBluescript II SK+ vector.
The determined cDNA sequences are provided in SEQ ID NO: 2, 3, 6, 7, 9, 12-15, 18, 19, 21, 23, 26, 28, 29, 57, 58, 60-66, 71-73, 78, 79, 105, 107, 119-128, 139, 141, 142, 149- 161, 186-195, 197, 198, 205-233, 252-256, 264, 273-293, 319-330, 366, 373-377, 382-385, 390-393, 399-434, 479-503, 507-512, 522-528, 531-547, 552-554, 556-573, 587-589, 592- 612 and 621-771.
Example 2 Isolation and Characterization ofcDNA Clones from Pinus radiata Isolation of cDNA clones by high through-put screening Pinus radiata cDNA expression libraries (from various tissues, including cell cultures, fascicle meristems, phloem, pollen sacs, roots, seedlings, shoot buds, strobilus and xylem) were constructed and screened as described above in Example 1. DNA sequence for positive clones was obtained using forward and reverse primers on a Perkin Elmer/Applied Biosystems Division Prism 377 sequencer. The determined cDNA sequences are provided in SEQ ID NO: 1,4, 5, 8, 10, 11, 16, 17, 20, 22, 24, 25, 27, 59, 67-70, 74-77, 80, 109-113, 140, 143, 162-185, 196, 199-204, 234-251, 257-263, 265-272, 294-318, 331-365, 367-372, 378- 381, 386-389, 394-398, 435-481, 504-506, 513-521, 529, 530, 548-551, 555, 574-586, 590, 591, 613-620 and 772-908.
Example 3 Polvnucleotide and Amino Acid Analysis The determined cDNA sequences described above were compared to and aligned with known sequences in the EMBL database (as updated to May 1999). Specifically, the 27 WO 00/22092 PCT/NZ99/00169 polynucleotides identified in SEQ ID NO: 1-29, 57-80, 105, 107, 109-111, 115-125, 135-139 and 145-904 were compared to polynucleotides in the EMBL database using the BLASTN algorithm Version 2.0.6 [Sep-16-1998] set to the following running parameters: Unix running command: blastall -p blastn -d embldb -e 10 -GO -EO -rl -v30 -b30 -i queryseq -o results.
Multiple alignments of redundant sequences were used to build up reliable consensus sequences. Based on similarity to known sequences from other plant or non-plant species, the isolated polynucleotides of the present invention identified as SEQ ID NO: 1-29, 57-80, 105, 107, 109-113, 119-129, 139-143 and 149-908 were putatively identified as encoding the enzymes shown in Table 1, above.
The cDNA sequences of SEQ ID NO: 58, 60, 62, 64, 65, 67-70, 72, 74, 75, 77, 78, 105, 107, 119-121, 123-128 and 139-143 were determined to have less than 40% identity to sequences in the EMBL database using the computer algorithm BLASTN, as described above.
The cDNA sequences of SEQ ID NO: 57, 59, 66, 79 and 122 were determined to have less than 60% identity to sequences in the EMBL database using BLASTN, as described above.
The cDNA sequences of SEQ ID NO: 61, 71, 73 and 76 were determined to have less than identity to sequences in the EMBL database using BLASTN, as described above. The cDNA sequence of SEQ ID NO: 63 was determined to have less than 90% identity to sequences in the EMBL database using BLASTN, as described above.
Example 4 Functional Identification of Cellulose Biosynthetic Genes Sense constructs containing sequences including the coding regions for UGP (SEQ ID NO: 23) and SUS (SEQ ID NO: 49) from Eucalyptus grandis, and UGP (SEQ ID NO: 24) from Pinus radiata were inserted into the expression vector pETI6b (Clontech Laboratories Inc, Palo Alto, CA). The resulting constructs were transformed into E. coli XLl-Blue (Stratagene) and induced to produce recombinant protein by the addition of IPTG. Purified proteins were obtained using Ni 2 4 column chromatography (Janknecht et al., Proc. Natl. Acad.
Sci. USA, 88:8972-8976, 1991). Enzyme assays for each of the purified proteins demonstrated the expected substrate specificity and enzymatic activity for the genes tested.
Enzyme assays for UGP were performed using published methods (Peng and Chang, FEBS Lett. 329[1,2]:153-158, 1993). The data shown in Table 2 demonstrates enzyme 28 WO 00/22092 WO 0022092PCT/NZ99/00 169 activity for the expressed proteins as compared to data from Katsube et al. (Biochemn.
30:8546-8551, 1991) and Nakano et al. (J Biochemn. 106:528-532, 1989).
TABLE 2 SEQ ID NO: 24 23 Katsube et al. Nakano et al.
Species P. radiata E. grandis S. tuberosuni S. tube rosumn Enzyme UGP UGP UGP UGP KGil0.121 0.126 0.130 0.180 SEM 0.020 0.002 n.a. n.a.
KM UTI 0.091 not done 0.076 0.170 SEM 0.015 not done n.a. n.a.
KMAT no activity no activity no activity no activity Enzyme assays for SUS (sucrose synthase) were performed using the methods described by Sebkovd, V. et al. (Plant Physiol., 108:75-83, 1995). The data shown in Table 3 demonstrates enzyme activity for the expressed proteins. The KM SucrOSe of E. grandis is compared with the data reported by Delmer DP Biol. Chen?. 247:3822-3828, 1972) and Nakai et al.(BioSci. Biotech. Bioch em. 61:1500-1503).
TABLE 3 SEQ ID NO: 49 Delmer et al. Nakai et al.
Species E. grandis V. radiata V radiata Enzyme SUS SUS SUS lv Sucrose T I I I jE"-N1 j .OD I 1O. /I VV1OI.VUU [SEM 0.371 n.a. n. a.
0.02 8 n.a. n.
SM0.0031 n.a. n. a.
WO 00/22092 PCT/NZ99/00169 A sense construct containing the sequence of the coding region for cellulose synthase (CEL; SEQ ID NO: 50) from Eucalyptus grandis was inserted into the protein expression vector pcDNA3 (Invitrogen, Carlsbad, CA). The resulting construct was transfected into mammalian 293T cells (DuBridge RB et al., Mol. Cell. Biol. 7[1]:379-387, 1987), and recombinant protein was induced by the addition of IPTG. Proteins were solubilised from membranes, and the level of CEL activity was determined as described by Kudlicka K and Brown RM Jr. (Plant Phys. 115:643-656, 1997). As a positive control for activity, native CEL enzyme was solubilised from mung bean (Vigna radiata) plants. The determined levels of CEL activity for V radiata are shown in Fig. 1. The levels of CEL activity found in mammalian 293T cells transfected with the Eucalyptus CEL expression clone were found to be similar to those obtained from V. radiata (Fig. CEL activity was absent in nontransfected control 293T cells.
Example Use of a Cellulose Svnthase (CEL) Gene to Modify Polvsaccharide Biosynthesis Transformation of tobacco plants with a Pinus radiata CEL gene is performed as follows. Genetic constructs comprising sense and anti-sense constructs containing a polynucleotide including the coding region of CEL (SEQ ID NO: 8) from Pinus radiata are constructed and inserted into Agrobacterium tumefaciens by direct transformation using published methods (See, An G, Ebert PR, Mitra A, Ha SB, "Binary Vectors," in Gelvin SB and Schilperoort RA, eds., Plant Molecular Biology Manual, Kluwer Academic Publishers: Dordrecht, 1988). The constructs of sense polynucleotides are made by cloning PBK-CMV plasmid cDNA inserts into pART7 plasmids, followed by cloning of the NotI-digested Insert-OCS 3'UTR-fragments from the pART7 vectors into pART27 plant expression vectors (See Gleave A, "A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome," Plant Molecular Biology 20:1203-1207, 1992). The presence and integrity of the transgenic constructs are verified by restriction digestion and DNA sequencing.
Tobacco (Nicotiana tabacum cv. Samsun) leaf sections are transformed with the sense and anti-sense CEL constructs using the method of Horsch et al. (Science 227:1229-1231, WO 00/22092 PCT/NZ99/00169 1985). Transformed plants containing the appropriate CEL construct are verified using Southern blot experiments. Expression of Pinus CEL in transformed plants is confirmed by isolating total RNA from each independent transformed plant line created with the CEL sense and anti-sense constructs. The RNA samples are analysed in Northern blot experiments to determine the level of expression of the transgene in each transformed line.
The total activity of CEL enzyme, encoded by the Pinus CEL gene and by the endogenous tobacco CEL gene, is analysed for each transformed plant line created with the CEL sense and anti-sense constructs. Crude protein extracts are prepared from each transformed plant and assayed using the methods of Robertson et al. (Biochem J.
306:745-750, 1995) and Pear et al. (Proc. Natl. Acad. Sci. USA 93:12637-12642, 1996).
The concentration of cellulose in the transformed tobacco plants is determined using the method of Smith and Harris (Plant Phys. 107:1399-1409, 1995). Briefly, whole tobacco plants, of an average age of 38 days, are frozen in liquid nitrogen and ground to a fine powder in a mortar and pestle. The cellulose content of 100 mg of frozen powder from an empty vector-transformed control plant line, at least one independent transformed plant line containing the sense construct for CEL and at least one independent transformed plant lines containing the anti-sense construct for CEL are determined using a glucan estimation kit from Megazyme (Warriewood, New South Wales, Australia) using the protocols supplied by the manufacturer.
SEQ ID NOS: 1-908 are set out in the attached Sequence Listing. The codes for nucleotide and amino acid sequences used in the attached Sequence Listing, including the symbols and "Xaa", conform to WIPO Standard ST.25 (1998), Appendix 2, Table 1.
Although the present invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, changes and modifications can be carried out without departing from the scope of the invention which is intended to be limited only by the scope of the claims.
EDITORIAL NOTE APPLICATION NUMBER 10837/00 The following Sequence Listing pages 1 to 264 are part of the description. The claims pages follow on pages "32" to WO 00/22092PCNZ9019 PCT/NZ99/00169 SEQUENCE LISTING <110> Bloksberg, Leonard N.
<120> materials and Methods for the modification of Plant Cell Wall Polysaccharides <130> ll000.l0l2clPCT <150> US 09/170,862 <151> 1998-10-13 <150> US 60/148,426 <151> 1999-08-11 <160> 908 <170> FastSEQ for Windows Version <210> <211> <212> <213> 1 455
DNA
Pinus radiata <400> 1 aatttcgggg aattcaatac tgaacgttct gggccgagaa gaaattctca gggactctct ccaagcggaa gaagcatgcg ctccacgggc ggtttctgat ggagtgtgct gggcattatc agaagagagc caaaccagct ttagtaattg cataaatagt agacgaaatg actgccgagt tcggcctctt attttcagga actttcagtg ctaggaggtg gttcctttgg aatatctcta gctggcgtta tcacttcgtt ctttgtgtga agcagaatat aactgacctg gagctgggac gtgccaatta agatc tggccgcaga caggccggta ttacagaatt taatagaagc tttagatccg tcgtctttat taggctgatc gttgcaaatt tttcttcgag ttcgcggatt acagtcgttt gtcgcgagtc ccactgacta gatatccctg 120 180 240 300 360 420 455 <210> 2 <211> 218 <212> DNA <213> Eucalyptus grandis <400> 2 gggccccagc tctcgcctcc ggcgcggctg tcgtgtctcc cagagccgtc tccgattccc ccagccgcag tgttctcgga attatactag tgactaagaa gagagcaaaa cccgccgtcc ccttcaagag cgtgcggaga gctccggccg ggaactcgca gacgtgcctt gaccccgacg gaggtggggc cgggacccqt ctttaccccc cgttggga 120 180 218 <210> 3 <211> 737 <212> DNA <213> Eucalyptus grandis <400> 3 gttaaaggat gcaataattt cccacggttg catagtgggt gaacgctcgc gcctcgattc gggtgcagac tattaccaaa cagaatctga gcctattggg atagggaaga acacaaagat ctttttgaga gagtgccgcg ttgaacgctc rggagttgag ctgaaggaca cggtgatgat aatagcttct ctgttagcgg aggggaaggt aaggaactgc ataattgaca agaacgcgaa 120 180 240 WO 00/22092 WO 0022092PCT/NZ99/001 69 gattgggaaa ggatgggttt tggtacagtt aaaatactcc agttctcgtg ctagtcctct gtacttctga tcgaactttc gtaaaaaaaa gatgtggcca tacatacgct atataagcgg gagaagagac ctggagagtt ccatagtatt gtttgtaaaa gatactatcc aaaaaaa ttgtaaacaa tgggaataac atggattttg ctagcatgga gctgatctcc agaggacaag aaggtaatct cggtgatgat agatggcgtt cgtgatactg agtcgatcaa gatcgaacaa tttctgctgt cttactagag gcgagatatg gtcttgtgaa gaagaagcag gagaaggcaa cagttgtttc gttatacaga atgtacaaaa tctgagttcc aataaacttt actgatgggc atcggccagg ccatagaaga caaggtgaaa gttttcaagc aaacttatcc cacaatataa gccggttgta tgttgtttaa <210> 4 <211> 202 <212> DNA <213> Pinus radiata <400> 4 gatacgaatt gtccttcagg gttttaactg ggaatctcat cgctctggag gatggtatca taagctttct gggaaagcag ctgaaatagc ctcaaaggga ttcacgattg tttggcttcc accacccact qattctgtct ctccagaagg ttacatgcca cgtgacttgt atgatctgaa ttcaagatat ggaagtctgg aa <210> <211> 472 <212> DNA <213> Pinus radiata <400> aatcaagatg ctcatcgtca tcagcatttg atgtcaccac aggttgattg atcctcaggg gtaacctact tggaaaatca gataagctta cacaaggata tatgatcatt tctatgattt acccgtgcgg gaatccattg tatgctgccc aaattgatga acggattgtt taagggaatt aaagccaact tgatacaggc tgcatatatt tggtctgcat ccgtagtact aaatttggtc aattggatta ctgcatgtgg ggtgtaatgg tcaacacagg ctcacacacc gataccataa ttgaaaattt atgaagttgg atgctacagg cattgcacaa gatggtggcc gacattggcc caggaact cc cagagctgat tccatgctaa ga caa tt tga aggatcttca tcaatattgg ctcccgagca atttcctcgt tacaattttc tgatgctcgc caatgaaggc ct 120 180 240 300 360 420 472 <210> 6 <211> 997 <212> DNA <213> Eucalyptus grandis <400> 6 acctactgat tctgtgtcac aagatatgga actattgatg aagagttctt ggagatgttg tatatggaat atttttggtg tcctcatttt cagggtagag tattgatcac tcacaagatt gagtgaaatt gggtatgatg tgtcaaagac tacttggatg cctcagctat acatatggag cgactggata aacgctacaa tcttcatgcc gcattggaaa tccaggggtt gtcggatggt tggctctacc cagggtcatt tatcttgacg cacccaggaa atctgaaatt ggttcactta ctgaagggta aactgaagga tgcttaatca 9tcgtctaaa gcaacaagag ttgtgcggaa gatoggaggct ctagtgaacc a aat ggatc a atggcacagc gatgtgaata qgccatctcg ggagatttcc Ctccagcagt tctctatcag tatgccgagg tctcgtgaag tcgatgtgca ttgggatgat tagtggagat agatctgaag cgattttgtt ttactttgcc caatcaagat.
tggagcatt t ttggcgacta agccgtaaca aagtggaaag gttctatgac gaatcgcaac gatctttaca aagttccatg caataccaga cgagctgtag aattttcatg gaatggttac agaggctttt gttaggtgagt gcacacaggc gatgtcacca tcagatcaga tttgtagaga gaaatgcaag cacatctttt aagatccat t atttaaattc aagttaacat atcaaaacgg ttgctgacga ctgccccaaa attggctgag ggggtggcta actgggattc agagaattat caaaaggaat agggaaaacc accatgacac gatatgccta ctcactacca gcaggagcac 120 180 240 300 360 420 480 540 600 660 720 780 840 900 WO 00/22092 WO 0022092PCT/NZ99/00I 69 tattaaaata accaaggctg agagggacgt ctatgcagcc attattgatg acaaagttgc aatgaagatt ggaccaggct actatgaacc ccaaagt <210> 7 <211> 856 <212> DNA <213> Eucalyptus grandis <400> 7 960 997 ctcttctcgt gtatcgccaa cccctgtgca gcttgtcaag ggaagaaaag gagagaaatg acagggattc gagactggga aagaagaagg gaagagaagc agcaaactcg ttccattacc atatgtgaga gtgaatagga.
tcagagcttg ctcgctttct gaactcattg acacttgcgg agtgcaactt cgtgcttgcg aattgggaag atgctagaca accctatctg ccccgacgaa aaattgctga caccataccg gagtgacaca t ttggt tcgc ttactcatgt ctgtgc catataaaga ggtcgcgaga agaggctgt t cgccatttgc ctgtggcact tcgatcgaca catcagcagt gaagaataga gg ct gag aa a tgcttcagag aaccgtaata tccagtcgac atactcatgg tgacagactg agtgaaagaa agattggcca ggggttgatg aaggcttgtg ccatttgaag atggcagctc gtttctacgt qtggagagct gaggctcaag ccactctcga ataatgcgat agcgcctatc gtgttggatc tctgcaaggt tacgaggata acatgatgga agaaaggcga tcgaatatga cgaactcgat aactcaatga tggatagtga ggaaggacaa ttccaccaga ccgttattcc tgatcatttt cattgtggct agttcccgaa atgaaaaaga ctccacttgg atccggggtt ggtcttcgtg gattaaggaa ggctgatgct tcctcaggac atacaatgat aaagaataag gcagcagatg cat tgccaaa ggcacttttc tacatccatc gtggtctccc aggtgaacct <210> 8 <211> 383 <212> DNA <213> Pinus radiata <400> 8 cttcctcgcc ttgtctatgt gccggtgcca tgaacgccct ttgaacctgg attgtgatca tttctcatgg atccccaact gatggtattg atcgccatga atgaagggtc tagatggcat aggcaagctc tgtatgggta ttctcgtgaa ggttcgagtc ctatctcaac tggaaagaaa tcgatatgca ccaggggcca tga.
aagagacctg tctgcagttc aacagtaagg ctgtgctatg aacagaaaca gtgtatgtgg gatatcagca taacaaatgc ccgtgcggga ttcagttccc ctgttttctt ggactggatq ccacaagaaa cccattcatt ggcaatgtgt tcagagattc cgatatcaac cgttttcaac 120 180 240 300 360 383 <210> 9 <211> 1860 <212> DNA <213> Eucalyptus grandis <400> 9 ccactacatc acttggaaag tgacagatat gatacaagga gtatgatcct gtgctczrgc tttgcttggg gaaagggtct tgagctagaa acctgtattc cacttcactt atggggcaaa aacaacagta aagctttgct gc taa tagga ccagtgtatg ccagtgtccc tgccgcggrg cctgttcatg ggacctgtct aaatcatcgc attgcctcca attaaggaag gagattggat aggctatcag atgttcaatt acatagtttt ttggaactgg aaaagcggcc gttcaaggaa cgaagaagaa ttgatctaga tcatgtctca cactaatgga ctatccatgt ggatttatgg ggaagctatg tcctcagagg ctttgatatc atgtgtgttc aaagatgaca gtcaaagtca aaagatgaca agacattgaa gaagaatttt agatggtggc cataagttgt ctccgttaca tgttttctaa ttcgatggca aacatgagag aatcggcagg tgtgattgct aagaagaagg ggaaagaact gaaggacttg gagaagcggt ttgccagaag ggctatgaag gaagatatct tggatcccca ttgatcgaca ggcttgatgg cattgtatgg ggccttcatg atgatacgag acttgaagaa agggttttga ttggacagtc ggactaactc agaaaacaga tgacaggctt WO 00/22092PC/Z/O16 PCT/NZ99/00169 caagatgcat gggatcagca ctccgttgag caaactgctt tttgcttttc cacgctcact tggcgtgctt attctgggtc qgtgcttgcc gtttggtgaa aatcttgaac atcgtggggc ccctttcctc gtccgtactt caagcaaacc ttcattcaac ttctgtctct tcttgattag ttgaactttt tgtagaggat cctataaatc attttcctca gagaggcttg tactgtacaa aactttgcga gaactacggt attggtggcg ggagttgata ctctaccttt atggtcggtg cctctgttcg aaaggtctga ctcgcctcta ggtccagttc acgccgtctc tact tgggac cataggtttt tgcaatatat ggaagtctgt tgtcagatcg gtcgtcattg cctatatcaa tacctgccgt gcatatggtt ggagtggtgt tatctgcaca ctaacttcac tcaagtggac tcgtcgccgg ggaagctctt tgggaaaaca ttttctcatt tcaaaccgtg tccgacccga atattgtaat gcttgaagag tctgctcaag atattgcatg actccatcaa tcctttgtgg caccattgtc ttgccttctc cttggccctt cagcatcgag cctcttcgct tgttacagca taccctgctc tgtttcggat cttcgccttt gaacaggaca ggtctgggtc tggggtggag ttagatgtgt gcactagggg tgtggaactg attaagcaaa cccaaaagac gttctgagat tatgcttggg taccctttca actgggaaat ttcctatcca gactggtggc gtattccaag aaggcagccq ataccaccaa gccataaaca tgggtgatcg cccacgatcg cggatcgatc tgctgattct cgctttacgg aaatcttccc aaatgtgcaa aaaaaaaaaa cagctttcaa ggg ct c ttgg gaggaaaact cttccattcc tcattatccc tcatagccac gtaatgaaca gcctcctcaa aggacagtga ccactctaat atggatacgg tccatctcta tggtcctttg cgttcctgcc ggcgtcggat agctgtttct gattgaaatc agtcctggtt aaaaaaaaaa 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 <210> <211> 400 <212> DNA <213> Pinus radiata <400> ctggcactat ttctcacttg ggcatgtcta ttgtatgtga aaattgtgtc ctattaatcg ccaactggcg acaatccagc actgctgatc cagagaaaga tctgctgatt acccagtgga ctcacatttg aagccatggc qcgagtcaag attatggttt ctccactgat agggagatca gccaccactg gaagcttgcc agaagctgca aaccctaata gctttctcat cttgctgttc gacttgcctg gtcactgcaa tgctatgtgt agctttgcag ccgacgcata ggctactgga tcaaggacaa gtatcgattg acacaatcct cagatgatgg ttggttatgg tcagttgcca gtttgagtca ttttgtctcc gtcaatcctt aggagctctt 120 180 240 300 360 400 <210> 11 <211> 619 <212> DNA <213> Pinus radiata <400> 11 gcttttagtc tctcagagaa gtcaactctc atggacaatg agaagctata catgtgatca cggatggatc tatggttctg aggatggcgc tccatttact caatctatca gatcgtctgc attCagoagg cdctgcccgt aagactggcc tacactaaca ctgtactctt cctgccatat tcttgcaagc atctatttca actgagatgg9 agtggagtg gctttgagaa gtggtgttcc gctgtggcta tgacagaaga gcatgcccaa accaagttct tgtgguatgg ccatcgtcta gtcttctcac tgctcctctt gtcgtttggc agagtcgacc cgaagagaaa cattIctaaca gagaccagca tcgttgggca atttg-gagca tcccctcacg gggagaattc catctccatt cagtcctcgg aatccagctt accgaatggg ggtttcaaga ttcaaggggt ttgggttcta ggccgtctca tCtcttcctc attattccta atcgtgacgg tgttcattgc cactcatcaa ggaaagaggt tgcactgccg ctgctcctat ttgaaatctt agtggctgga tgattgccta cgctgagtaa gcgttctgga <210> 12 <211> 719 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 12 cttgctttga gacatgatcg gtgagtactg tggatccttt atacttgccg tggattaccc gcgatgttga cttttgaagc ttctgcaaaa agcacaacat gattacttga aggacaagat gaatatgagg agttcaaggt gaagaagggt ggacaatgca cctggaatga tacaggtttt ctacctcgac ttgtttatgt gctggagcca tgaatgcttt ttgaatgttg actgtgatca tgaaggggag gaaagaacct cgtcgacaaa cctctctgaa tgagccacga acagccttct gcgaatcaat ggatggcact cctgggccac ttctcgtgaa gatccgggtc ttactttaat ccttcacagt cctctcataa gtgtcgtgtt actgctgagt gctcctgaat tttgtaaaag gctttggttg gcctggcctg agtgggggac aagcgacctg tcggctgtcc aatagtaaag tggctcctgt cagcgaacac atgtctctga ttgcaaggaa tctactttgc aacgcagggc caaaggcaca gaaataaccc ttgatactga gtttccaaca taaccaatgg cattgaaaga tgatgtcttt tgtcttatcc tgatgggtct gtgggtgccc ccagaaaata aatgaagaga aaagatgcca cagggatcac tggaaatgag tcacaagaaa agcgtatctt agcaatgtg 120 180 240 300 360 420 480 540 600 660 719 <210> 13 <211> 759 <212> DNA <213> Eucalyptus grandis <400> 13 attagctgtg gctatgagga tctgttacag aagatatttt tattgtatgc ctcctcgccc ttaaaccagg ttctcaggtg cctatatggt atggatataa acaattgtgt atcccctcac tgtcttctca ctaacaaatt attctccttt tcgtctcaat agcattgagg attggtggag cttttcgctg tgttccaagg gtcacttcga aagcaggcga acatcgcttc tcattcctcc ggtgtgtctt atgcgattaa caagactgaa gactggattt agcattcaag ggccttgggg tgggaaattg ctcaataccg tattatacct tttcacgact gaatgagcag gcttcttaaa tgaggatgga gaccacagtt cagcgggtat tggggcaaag aagatgcatg gggtctgctc tcaattgaga aggctcttgg ctgattgcgt gagataagca qgtattcttg ttctgggtca gtgcttgctg gactttgctg cttatcgtga cagtcatgg agatcggatg ctcggggctg ccatcaatct ttttgctaag agagattggc attgtattct actttgctag agctccgttg ttggtggtac gcattgatac agctttatgt acatcattgg gatatatggt gatttcaatc ttctgatcgt cagg cat tg t gtacatcaat ccctgcattt catgtggttc gagtggtgtc atccgctcac caacttcaca gttcaaatgg cattgtagca 120 180 240 300 360 420 480 540 600 660 720 759 <210> 14 <211> 1407 <212> DNA <213> Eucalyptus grandis <400> 14 cagtgctttt ccatccttgc gatgatggtg ctgcgatgct aagtgggtac ctttctgcaa tctcagaaga ttgactactt gcaatgaaaa gagactatga cagaaagcgc cggaggaagg tctcgtgacc accctggaat gagggaaatg aacttcctcg catcataaaa aggccggagc gctccgtaca tcctcaacct gaagcaatgt gctucctgat cctcagagat ttgatggtat tttgatgtta acatgaaagg tgtgttttca ataggcaagc aagccctcct catcatgctc cccactaaag atctctcaga atcttcaacc ttggagagat tgtggactat ctcattcgag aaagtattcg gaaggataaa ggagttcaaa ttggtctatg gattcaggtt gctggtgtat ggaaaatgct tgactgcgat ggatccucaa cgatcgcagt gctggatgga actttacgga ctggtgtggc agtttaccga cgacaattat cctgttgata tctcttgttg attgagcccc attcagcctt gttcgagtta c agga tggga ttcctcggaa gtttccagag ctggtgagag cattatgtca gttggtagaa gatcgatatg atccaaggac tacggacccc tgctgctctt gactcaaaaa gatgagcarg aagtctcttg aaacggccga gtgcgcctga cttttgtgaa atgctttggt caccttggcc gtagtggagc agaagagacc tatctgcaat actatagtaa atttgtgcta ccaaccgaaa cagtttatgt cttctatgcc gttgctgccc gggaagacct agagatcaat ttatctttct ctttgcaaga gttctacttt ggaacgaaga agcaaaagcg tggaaataac ccacgacatc cggtttccaa tctgacaaat tgctgttcgt tgtgcagttc taccgttttc cgggacagga gaatttacca ttcaaagaag aaatgccgcc gctgatctct 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 WO 00/22092PC/Z9016 PCT/NZ99/00169 cagatgagct gcaaatggag gtcattagct ggctcggtta gtctattgca agactgcacc tgtccacttt ttgagaaaac gagttcctga gtggctatga ctgaggatat tgcccttaag aagttctccg ggtatggatt ttttggcttg atctgcccat agagaagacc cttaacaggg gcctgcattc atgggctctq tggcgga tccactgtgt ccgtctatgc gcatggggca ttcaagatgc aagggatcag ggt tccgtgg ttattgagtc tgatcaaaga aagagatcgg actgcagagg cgcccattaa aaatattctt gacgctgttg agccattcat ttggatatat atggaggtcg tttgtcagac gagtagacat 1080 1140 1200 1260 1320 1380 1407 <210> <211> 2913 <212> DNA <213> Eucalyptus grandis <400> gccagaaggg ggaggccgag cctcattttt caccatcctc ccctccaatt ttcttttggc tcgggatttc ctgccttcgc catggctgat cgcatgttga ctctgctcac cgcaacgata catcttgcag cgccaccaga aaagcttctt gatggggcct gcctccatgg gttgctcttg gaacgtccat gcgcttgttc agagcttgct gatggaagct cactgcctct tttccgcgcc caatcgccat ctctccgcta attcctccaa gtccactgct tgtgttctcc ctccaacatg cgagaccccg tactcccagt gggtgacacg gctgagcgcg tcccgacccg tgcactctcg gatcatgtct ccccacggat tggccaggtt gtttacatcc cattaagcaa caaggactgg agacgcggtt ggaaccacct ccacattctt cgcgtcccct gttcgaggtg tggccctatt agagttgcag ggcaagcctg ttccttgtta gcacataaat gacgaagtac ccagagtcag ttgccagttc actgctgatc cttccaagaa attgctggaa cactcttcct ggactctacc cattgtttca ccaggtgctg gttgaaatcc ttccaccctg acaCtLqLyt yggtgaaag ccgtgtcaag aacttgacag actcgccaac ttggtcgtgg gcagtctgag atgaagaaaa caggtggatt tcctcc caga tgacacgaag ggagtcttcg tgaggccatg acttgtggat cattgtgcat ggcaaatcg gcttcttgta gacttcttca aaagggtgcc atgcagagaa gttgaacctg actgccgtgt Cctcttgctt cgttcgttcg gtcccgtttt cgcgccattt ctcgaagcca ttgtggcctt tttttgctga ttggtgaagt ctgttcgtcc ttgagcaatt tgaatggtaa cgactctttc agctcttcca acaaggggaa tcctgaggaa tcgagcacaa tcctcgagat agaagttctt actttgctca tggat caagt atattactcc gtggccagcg tcagaaatga tggaaagata atctgatcat taggtgttac acatatactg tcatcgccat gcaaggatac gagttgtcca acatgagcat agatcgagga ataagaagaa ggcttgttga ttggaggtga tgtacgacct tgaccqL ttcaaccggc tgccaacctt gctaccacat acaagtgcaa t tgaagaaaa atggcttctg tttttcgtgt ttcccttccc tggggctccc gaagttcttt cagccttcgc cctttcaagg gtttgaggcc cctcaaatcc aaggccgggc ggaggttgct ctttgtgctt caagtctatt tgacaaggaa gaacatgatg ggcggaggag gttccaggag gatccagctc ggatagggtt ggacgacgtc tcgtgcccta tcggattctc cct tgagaaa gaagggagtc cactgaggat cggaaactac acagtgtaca gaagaaattt gaaccacacc agtggggcag cgggatcgac c tact t tgc t act cctcttc gcctattat gtggtatggc caggaggaag catcgaaaag 9ac~aatqga tatctataaa tgccacttgc tgatccttac gattgaccag gtatacatgg gaagcatgtg ataaaagggc tttccattgt ttgaagatct ttctgagaga gagcgtttgg gttgaagcca atctctgagg actcaggaag gtgtgggagc gagtatctgc gagcttgact ggcaatggcg agcttgcacc gtgaatgcca tatctgacct atcgggctcg ctgttggatc cccatggtct cttggttatc gaggaagaaa attatcactc gtttttggga gtccgcaagt gtcgcgagcg agtgatggaa atagcccatg gaggaaaagt gacttcatta tatgagagtc gtcttcgacc tacaccgaac agcgatgttg ttcaccatgg aagaactcca gattcgaagg tacaagctga gagctctacc 9ctttcgggt aatggtggac catggtgacc tcccactggg aaaatatatt actaaccttg ctcccccatt tgcgtttaag cctcttcatt agaatttaga acgagaccct agggcaaagg agagcagagc cgattgtgtc acatccgtgt acttcaaaga ttgagccatt tcgagtttct ctctgcttga gaatccagaa cgctcaaacc agcgggggtg tccttgaggc tcaacgtcgt cggataccgg tgcttcaccg ggcttcttcc ccgagtactc ggatttcccg aacttgctgg acattgttgc ccctcgagaa accacttctc tcaccagcac acatgaactt cgaagttcaa aggagcggcg agaacaagga caaggctgga agttgaggga acttggaaga atggccagtt gctacatctg tgaccgtggt cagctgagat aggcggccga acgagatctc ctgagaggct atcoggcgcga 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2160 2220 2280 2340 2400 2460 2520 2580 WO 00/22092 WO 0022092PCT/NZ99/001 69 gagtcgccgg tcctccggct ttttgaagtt atctttttcg ttgcattttg caatcccact taccttgaaa gtcgagtaaa ttcaaggaat gggtttgtac ccgtatttcg tgttctatgc caaagagaca aaacattgga ttggtccttt ccatcaattc taaaaaaaaa cctcaagtat cgcccactgg cacagtctgt gattgttacc agaagacgga agcattggac aattgtttga atttgggatt gccaagagcg ttctcttctt tgtttccatt ccgcgaatgt agtaaatggt tcattttctt ttcaaattgt aaa 2640 2700 2760 2820 2880 2913 <210> 16 <211> 401 <212> DNA <213> Pinus radiata <400> 16 ataaaacaac aagggttgga gaggcccacg gtacaacatg ctaatactcc gggtgccctt tttgatgtgt ggccttactt gagctaaaag gacaacctga tctttgattg cgcataaaca accaaatacc cagattcaga cattacacct caat caacga cagaacagaa ggaaagattt tctgattatt aggaatcaca catatattgg cagattattg attgaaaaag aaaggagttt tcagaggatg ggaaattaca cagtgcaaca aaaaattttg tggtgactag ttagcggaac tgcgtaactg taacaaatga gtgatggaaa tagcccatgc a gttgattcct acaacattcg ggtttctcga agtaactgcg tttagtcgca cttggagaag <210> 17 <211> 477 <212> DNA <213> Pinus radiata <400> 17 acatggaatt gatgtttttg gatttatttc ccttatacag ggaactcttg ttcagtcccg aaagcccatt atcttttcta ggaatggttt gcaaagagca tgatattgac ccatcaaagt tagactagtt aaagagtata tagagtgcgg aatggtgagc atcccaagtt aaaagcagca aacagacagc tggcaagact aacgcttgag ccaaagacag acctgaatgg tttaccgcta caacattgtt tcgccttact tgaacatatg cgaccgggtt ggagctggtt agaagaagtg tcagttcaga catctgtgac t ct cctggag actttacatg tgtgctttga aaaaatatga aaccttgtcg gccgaaattg tggatatgtg accaggggtg cagacatgca gtacaattga atgaccgtaa caggattggt tggttgctgg aaaaaatgca cacaaaagaa cctttgt 120 180 240 300 360 420 477 <210> 18 <211> 503 <212> DNA <213> Eucalyptus grandis <400> 18 aaacttctaa agcacggcgg ggattaagcc atggcggatc cgaggccctc tttgctgacc gggcaaaggc atcttgcagc gagcagagca aggcttcttg gattgtctcg tctccatggg catccgtgtg aacgtccatg cttcaaagaa gcgcttgttg tttggcgg cgacccttcc gtttcacttt gtgtgctgaa gcaacgattg gccaccaaat atggggcttt ttgctcttgc cgcttcttct atggaagctt acg ggtggttcgt ccgaagtcac tctggtcttc acttgctgtg tggtaaagtc tgttcgtgca tgagcacttc gaatcctgat gcagttttgt agccctcgcg ctgtcaaggc tttgaggcga ctcaaatcca aggccaggtg caggttgatg tctgagccac tacgagagaa agcgtttgga ttaaagccaa tccccgagga ctcaggaagc tgtgggaaca agtatctgca ttactgccac 120 180 240 300 360 420 480 503 <210> 19 <211> 413 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00 169 <400> 19 atcaaaatgg gcacgatggt tgcttcgtta ataaataaat taatcagtga gtttcaatac ctgttcgtcc aaggccgggc ttgagcaatt ggaggttgct tgaatggtaa ctttgtgctt cgactctttc caagtctatt cctcaaaatt atagcatcac act caggaag gtgtgggagc gagtatctgc gagcttgact ggcaatggcq acaaaatctc taggaacctg cgattgtgtc acatccgtgt acttcaaaga ttgagccatt tcgagtttcg ggatctgtct tccagtacca gcctccatgg gaacgtccat agagcttgct cactgcctct caatcgccat gtgtcttgct cgtctcactc gttgctcttg gcgcttgttc gatggaagct tttccgcgcc ctc 120 180 240 300 360 413 <210> <211> 1108 <212> DNA <213> Pinus radiata <400> gtgatctcgg catccgaaat tgctccgctc tgatcggctg gcataagtgc tttctatcac gatcggatcc aaggaagggt tgggaaaaaa ttgggagaag aatggcggtt aatggcctaa gattccaaag attgtagaaa cca c gcat gg gcatggtacc gatgaactac gcaatagttg aaagtttaaa cgccactggt tttcgtgact caactaaaca agaggattta gtaacagttt gaccgaaaat ctaaacttca ttgtgcgact tcaagacccc at ccat cgg a tgggaacaac catttctgga ttccgttggt agtactctgg tggcagaaga c acctgg cc a tctcacaggg atctcagtat cttaaaattg tt tgggtgag gcacatcttc taaagggagg tatgataatt cgtcttctac ggctgcagca agccgaggtc ggtttctcgc gactgacgag aaccaaggaa catgggttgt cttgattgtg gctgatgaat ctcaaatatt tttgacacca cggagatgtt taaggagtac cctatttgca tgtatagc acatcttggt ttcgcataat tggaggagga cttcgctatt gcacgctctq ccagcagttg acgaagctga tacctcagcg atcgtggttc ctcttggaca actgggccca aagcaaatag tcatttaata gacatccaca tggccaacta ttccccgctc gtgttcatag cttgtgtttt ttccagagcc tcgccggaat ggtggtggca caacatagcg atctgtccgc cttcacccgc accagatcag gcgaagaaga cctacgatac agcttgttgt aatccgtcat agtctctgaa cacatgatga tcttcaacca aaggtcgtac ttttgaacag ctaactcaga aacgtatttg cggcggtgga atactctcat acactacgat ttaacaggaa gacggggata agccgaaact tgataatgag gaagattgaa gttggcagct gttaaagctt tgaagtacga caacaaatac tacaattaag gagtcaatat agacaaagaa tgggaagctt caacttggga ttattatctc 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1108 <210> 21 <211> 559 <212> DNA <213> Eucalyptus grandis <400> 21 cggccgagat cctcctcccg tcctcctttt ttctctccac gcccgccaat tcaaatcggc acctcgtgtc cgcccactga cagcgaccaa ctacqatgqq tggttacact tttcgtacc ttttatcttc gttcatctcc ggctgccgct cgtctccggc tcgctatctc cgaaattgtc gagcct t tt g ttgtactqq tgttattctc ttcactcatc cacatcacca atcgctgtct gccaccctga ctcggccaaa agtggcgaag gtcccttacg gacaaactcg ccccccgtcg tccctcccca ccttcctcct ccctctcaga gcgcccccga tcagcgagag ctcaacatgt atagcttggc ttgtgctgaa ctagccggcc aaagcccaat cctcctcctc tccggagctc cgctgcgaag tgagaagaat tgactggagc gcccactccc gctcaatggt acttttatat cgaatctcgc ctcctcagcg ttcttcgcta ctctctcagc ggattcatca aagatccaga caggatcctq ggtttgggaa 120 180 240 300 360 420 480 540 5S9 <210> 22 <211> 1036 <212> DNA <213> Pinus radiata WO 00/22092PC/Z90I6 PCT/NZ99/00169 <400> 22 gctaactcag acaacttggg aataaaaatg aatactgcat acacttatct cttatgaagg catgttggcg aattcaagtc gtgaacttga aggcgattaa cctaatccaa aggaagtgga gctataaggt tttttgatcg gtgaaggcaa cttcagattt tttgtcatcc gaaatcctgc gaattcaaga aggttggcaa cttgatagtc taaaggtgtc aaagttataa ttgaagcaaa gaaaacaagg tagtgagcag tggcattctt gatttttggg gagtagggtg gaagtttcac cataatttta ttataactag gaaagcctag gatgaagcat tgaaaaaaaa aaaaaa agcaatagtt ggaggtcact cagagt tcag gattgaaaag gaggcttgtg tggggtaaag tgcaattggt actacttgtg tagagtcaac ctttctaaag aggtgatgtg gcagggggct tcctgatgat ttgtttgtag ctctggtttt ccagtttcta ccaaacacaa gatctcaaaa cccaaaacgc cttctagaga ttcaaaatct gaggctgatg gtgcttcagc gtcaatgttc cagtcggatc cctacaaatc cgtttcaagt tggtttggca acacttgaga attgtgaact agctagaata atcttccata tgtaaatgtt taaataaatg ttttgaatca ttgcagatgt ttgcacaggt tcaataccaa ctcttaaaat ttgaaacggc caagatcacg tttacactgt ccaccattga ccatacctag gtggagtcat tacctgatga agcttataag ctgtgtttag atccttactg gagcgcgaat gtgctttgtt tctggtcaaa aaaaggtggt tccagaggag taatttatgg ggagataatt tgcaggtgct ctttctccca ggaggaaggt gttgggtcct catcattgat cctgaaggga atctgtgata ttttggcagt tgcccttaaa ggaggggttt ttttatgcat gagatttttt 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1036 <210> 23 <211> 467 <212> DNA <213> Eucalyptus grandis <400> 23 gtcacctttc ccttgagagt ctatcaagct caataatgct ttaaactcag ggcccaattc tcaacaaaac catccaccag gctttcactg gaaggaacct ttgattgctg caccagctgc ggattaggaa tgatctccat aagttaaccc acaagttgtt aatgccagtg gggaattgac gattgaagga agtataaaga cgaccgaggt agtttctagc cttaagagct tgtattgaaa ccaaaccata ttgaatctgc tttgagggat tcggactgaa acattgatgc tgcagaactt tgagct tcga atcttgaact catcaccaga tcaagaaatt ttgttctagc caagaagcag caatagcatt taattccatc caagcctttt tctcaat caccttcagg gccaaccttc cttgtttcgt atctgaagtt gtcaaagaac cacttccttt aattgccttt <210> <211> <212> <213> 24 704
DNA
Pinus radiata <400> 24 gttttgttga gctactagat tcgatttcag ttttagttac gtgcgaagta tgtgatttgt accccatctg aagactctga aagtcaatca tacaaatatt aqctactttc aacrgtacga gactttgaga aagctgtatt tctcatggtg caataaagaa gctcgatctt cggctgaact tcccga aagacgtggc cttgtaagtt catatcggta gcaaagaagc taagtgaatc tttagtaaat at t ttggtga cgagaagatg acgcctgagg aggacataga agaggatctc cctttgggta gtggaatgca attgatggtg tgcacataca tgaaggtccg tataaccgag caagaattca agttggtcga tcaacaatta aaggcttttg actgcagcac ttgaagcgat ctagatccag aagaatatat aggcaagcat agt9gaaact gaagtggata caaaaaagat tcagctataa cctgcattgc tagtgccagt aagggtgggg aacgcaaagt tagaatctga ctgaacgtga cgcttttaga atcatattcg tCcu9Laagtt tgcatttggc aat attgaggcat tgaagatatc tccaataccg cacaaatgag aatccgtcaa gctttcaagt tgcggtcata aatttccagt ggacaaaaag gctgg~agca aagttatgaa <210> <211> 712 <212> DNA WO 00/22092PCIZ9016 PCT/NZ99/00169 <213> Pinus radiata <400> gtggatcaaa gaatcccatt gagaccataa aaatggctac agggactgtg aggaaatcca atcgaaatac ttggaagcaa gcaatgtata aggaagacct aaagccatcg tcttatggat gccctggaag gatggtgcac tccactcaaa tcgttaagat gatgatgtta tttgcaaaac gcacaccgat gtgaatgtaa ttgtacgaag cgggcgaagg cagcgaggag agtgctccca ctctcaaaaa ttgttcttgt tctcgcttgc aaatgagcag gtgcaagagg gcacgagcca agatgatcgt tcgtgaagct gaatggtccc aggagttgac acggtgtgga agtgcacgca ctagtaccac gctgtctcgt aaaggtctgg cagcggaagg ctggagaagg gctgaccggg gctctaatcg taccagaaac tttcaaaagc ataaacaaag accgatgagg atatttgctt agtttgggat gcggcgtcaa gaagtgacga agataagaga agcttcatgg acgctatcat aagtgatttg gatatcagag tact tct tgc ctagatgcga atacttttat gcaacaagca ctcccatttt ccccgtggaa ggagaagata aacctattat caaattagag tgctggaacc cacaaggtcc gtgcctcgat actgctcaaa tgccaagatg ccgcatattt aa 120 180 240 300 360 420 480 540 600 660 712 <210> <211> <212> <213> 26 789
DNA
Eucalyptus grandis <400> 26 atcgtcttcg gctcctcgcg gagagagaga gaatggcgac gccgagcagc tccaaaaagc atactgcctc acagaaacgc tatggggaag atcttctcaa gtgcttctgt ggaccctgga aagagattga cttcgagcaa gagttattca tggtgaggca atcgcatatc acactactgg cggtatgagg ggcctgaggt gagaagattc acgagaaggc agtaaagctc agcttaatgc aacaaggatc tgaaggctga aagtgcttg atatcaccaa tat cgcggtg tttcgcagga agcgcagcgg agcgcttgac tcctgcggag ctgggttctc ggcctatcat ggatttccgc gaacatgaca ttacaatcat aaccctcaat tccaaatgat cttgcttccg ccaccgtcgg tgggggacga aaagtaatcc aaggaactct cgtgatgcat atggaaattg gctcgttata aagctgcttg ttggcgagat gatgagctca tactacaaca gaatttctga cacagagaga ttccgtctcc atgaagatct gacaaacata ctagtgactt tcttgtccaa cttgcacgag agaaatctct ttcctctggc cagaggctaa tcagaattgt atgagtttgg aactgctgag gagagagaga ggctgaggat gatcatatcc tgccgagaca tgagagatct tgaagctacc gtcttcaatg tgaagaagac aagtaccttt gatacttcat tactacaaga gaatgccatc atcagcaatt 120 180 240 300 360 420 480 540 600 660 720 780 789 <210> 27 <211> 2132 <212> DNA <213> Pinus radiata <400> 27 ataaatcagc tagtccgatc atacggttca LqataCatCL tgtgtttgca aagagatgtc gcctgaggaa gac ac a gna a ggaagatttc cctctggtca gtggagtcca gtggtcggtc ttctcatact tgaaggtcca gtcgactcca acatgccaat tatttttctc cgggtcatct aacaattata ggcctttgaa cncgcagcac ctgaagcgat ctggatcccc aataacaggt agacaagcat catggagact gaagtggata ctgaatgacg agtatagtcc atgaatacca Lgaaagguac gagatattgt gtgccagcgc ggctggggca aggagag tgcaat caga cagaacgaga cacttctgga atcatatacg tccgcaagct ctcgcttggc cgtttcctgc catcttggaa agatgctgtt ttcaggugtt gggagaagag cagctccatc caaatgagaa a a j-I-Caa actcacacgg tgcactttta aatctctagt ttacaagaaa gctagttcaa aaaatctgaa aattttatac tctgatgctc gcagtttctg rtggagcaca atcaaatcag cccagttgaa gttgattata gattttgaga gcctatgaat gctcgatctt tctctggaag cttgtaagtt gctaagcaat gttttccatt atataaaat t tgaaaaaaag cacttaccat gtcttgttgt gactctgaac gaaattttgg agttgtatga gagctctgct cgataaagaa caactgagct aagacgttgc cttaccgata tacatgaagc WO 00/22092 WO 0022092PCT/NZ99/0oI 69 tataaaggac ggcacagttg ggacttgaag gcatttgctt gactccactg tgccaatagt ttttctcatg ttgaagttga gtcatctgag aattatagtg ctttgaaggc agcacagcgc gcgat tgcaa tcccccagaa caggtcactt agcatatcat agacttccgc ggatactcgc tttcggtaac cactttcaat ggaagccaga aaagatacct aaggctttcg aatgccactt gaatgggaag tcctgaaaga aatgacgcgt atagtcccat aatatcaaga aaggtatttc atattgtggg ccagcgccag tggggcacaa agagcaatca tcagaactca cgagatgcac ctggaaatct atacgttaca aagctgctag ttggcaaaat gaggaactca tattacaaag agacttcctg ttgcaaaggt gtaacgagga tcaattatta ccagaagact tacctttgca ttcctgcaat cttggaatct tgctgttgca aggtgttttg agaagagatc ctccatcccc atgagaagt t ggcaaacgta cacgggattt ttttagccta ctagtgctcg agaaatctct ttcaacttgt ctgaagctaa ttcgcataat atgactatgg gagtcactac tttgaggttg actcattcgc caaagatgac tcctggagtc aaggttttga tttatacgtt gatgctcata gtttctgtga gagcacatac aaatcaggtc agttgaagac gattatagaa cactcagttg tgagagagct tgaatcgata atcttcaact ggaagaagac aagttcttac gcaattacat aactacaagg tcatcatatc gaattgttat gc ataataacta tatggtcatc actacgaatt ggttggcata t tcc at ttag taaaattata aaaaaagtga ttaccattgt ttgttgtaag tctgaacgcc attttgggac tatgaggaag ctgctcctct aagaagtgga gagctgtggt gttgcttctc cgatatgaag gaagctataa agtaaggcac aacaaggact caaatgcatt caaggagtaa atatcaacaa gttatcaaat aatcagcgtc tccgatcaca cggttcatat tacatctgtt gtttgcacgg agatgtcaac tgaggaaggc acagaaccgc atttcctgaa ggtcactgga gtccaaataa cggtcagaca atactcatgg gtccagaagt aggacaaggc agt tgaatgc tgaaggaatg tgctttcctg 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2132 <210> 28 <211> 2588 <212> DNA <213> Eucalyptus grandis <400> 28 ctgactctct ctctctctgt gaacgcaccc acacgatctt actgccccct gtagccgatg aaatgagaag ctgatcatat tcggcaaacc tatgccgaga taccaatgat ttcgagaggc gtacttggcg aatgaagcga agcctgcacg aggtctccgc caagaagtcg ctggaagagg ggtacctctt gtgagctcct agcagaggct aagatactcc caataaggat tttggctact aaaatgagtt tggaaatgat ttactatact gagagctaca ttcttcgtct agccatcaat ctaccagggc cgaggttgac tccctctcga tcgtgcccat gcattgattg gccacgtcga gcaatatctc tgtttttggt gtgtagttac caacatgcct cttataatgg cgttggttta acrgctagta agtgatcgtg ctcctccctc ctctctctcg ccctcaacaa tgtcgactct gagcagctcc ggacagcctt ttgggtcata ggaatgcggc ggcgaggacc tcctcaaggc gtcctttggt cacttgatcc tttgtctcct ccattccctc actgcgagca ccatattggg cttacggcga tggtggtcct cgaaaagatg agcagttgct acgtcggtca accgttatga acgagaagat aggagcaaag atcaacaagg gtaaagtgcc aagcgaggaa atgaagttta tgtcaaggac ggcttgattt gtggtttgag ccccagttgt tgtacttatq aaaaaaaaaa ttttcgcttc caccgtcccg cgcaggatgg gcagaggaag attggacaga ggc tga acg t ccctcctctc aacaatgtcg gctccggaca tcataggaat ggacctcctc ttggtcactt gacttcaagc tatggcaaga ccacacaact tggagatgag ctcagagaag cacaggtcaa atttgaaaac tgactcgccc cagatgaagg taagtgagga actactggag acaagtactc gcatttctat cagttgtagc agaagtcttt aaaaactgac gtcgtgaacg cagccactgc ggaacaaatg ctgattcggc gaacttacca gatgcgtact tctcgttttc actctcaccg gccttcgcag gcggcgcaga aaggcattgg gatccggctq aaccaggttc caagcatatc ggagattttc gtgaatatga gcttatggcc tgctacgctq tgatccaaaa tgagaagtat ggctctgacc gtaccagagg actatgaaaa atgaagctat tttccttgct tatgcgaagc gattttgatc tctctctctc cacccacacg cccctgtagc agaagctgat aaacctatgc atgatttcga tggcgaatga gcttcgtcgt tcccgcagcc gatggggaac ggaagctgat acagagaact aacgtgatgc taatggaaat atgcccgata gtaagttgct ctttggcaaa atgaggatct aatcactaca gacgcgttcc tttgaaaagg agagtagttg aggaatagca aatgcttctg cctggtggag ttccaacaac aaatacactt tttaatcaag tctgttttgt atcttccatt cgatgactgc catatccata cgagacttac gaggctggtg agcgacgaaa 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1:320 1380 1440 1500 1560 1620 1680 WO 00/22092 WO 0022092PCTINZ99/001 69 agatggactt ttgcttatgg ggtcaccaca tatgatggag aagatctcag aaagcacagg aaggatttga tgcctgactc ggaacagatg tttataagtg gacactactg tttacaagta gaggcatttc tgtcagttgt atgagaagtc aaaaaaaa caagcaacca caagacaagc caactggaga atgaggtgaa agaaggctta tcaatgctac aaactgatcc gccctgagaa aaggggctct a ggag ta cc a gagactatga ctcatgaagc tattttcctt agctatgcga tttgattttg ggttctaatg atatcatgcc ttttcgtaag tatgactttg tggccatgag gctgaatcac aaaagacgcg gt at t ttgaa gaccagagta gaggaggaat aaaaatgctt tatcctggtg gctttccaac agcaaataca atctttaatc gaaatagcct cgatacaaga t tgc tggta c gcaaaagcag gatctcataa tacaaaaatg ttccttacta aaggtt ctt C gttgctacca agcatccctc ctggcattga gaggcaatat aacgtgtagt cttcttataa aagactgcta gcacgaggtc agtcgctgga ctcttgtgag aggctaagat ggattttggc agtttggaaa tactgagagc gtctagccat gggccgaggt tcgatcgtgc ttggccacgt ctctgttttt taccaacatg tggcgttggt gtaagtgatc tccgcagcag agaggacgtc ctcctaccgt actccacgag tact aggag c tgatatcaac tacagtaaag caataagcga tgacatgaag cattgtcaag cgaggcttga ggtgtggttt cctccccagt ttatgtactt gtgaaaaaaa 1740 1800 1860 1920 1980 2040 2100 2160 2220 2280 2340 2400 2460 2520 2580 2588 <210> 29 <211> 627 <212> DNA <213> Eucalyptus grandis <400> 29 acaatgtcga agctccggaa gccataggaa aggacctcct ttaatttttt ttcattccgt gcgtacttgg atagcctgca tacaagaagt ctggtacctc aaagcagagg actctcaccg agccttcgca tgcggcgcag caaggcattg ggattcatcc atcgtcagag cgaatgaagc cgaggtctcc cgctggaaga ttgtgagctc ctaagatact tcccgcagcc ggatggggaa aggaagctga gacagagaac catggaagac gctggtggtc gacgaaaaga acagcagttg ggacgtcgct ctaccattat ccacgag actgccccct caaatgagaa ttcggcaaac ttaccaatga gtgtccttct ctttggtcac tggacttcaa ctcatggcaa caccacacaa gatggagatg gtgagccgat gttgatcata c ta tgc cgag tttcgaggtc ttctctcaga ttgatccggc gcaaccaggt gacaagcata ctggagattt aggtgaatat gactgcgagc tccatattgg acttacggcg tgatcttcct ttaatccata tgaacgtgat tctcatggaa tcatgctcga tcgtaagttg ga ct ttggca 120 180 240 300 360 420 480 540 600 627 <210> <211> 151 <212> PRT <213> Pinus radiata Phe 1 Val1 Phe <400> Arg Gly Ile Ala Asn Leu Arcg Pro Val Gin 5 Asn Tyr Arg Arg Asn Gly 10 Glu Trp Arg Tyr Gly Arg Arg Val Leu Gly Thr Ala Glu Phe Leu Arg Ser Ser Leu His Ala Ile Gly 40 Ile Ser Gin Gly Leu Ly s Phe Thr Ser Ser Ser Ala Arg Lys Lys Cys Asp Tyr Phe Arg Lys Val Ser Asp Arg 55 Gin Phe Ala Asp Ser Ser Asn Ile Asn Thr Val Val Pro Arg Ala Thr Phe Ser Ser Glu 90 Ile Thr Cys Leu Asp Pro Val Ala Ser Thr Arg Leu 115 Arg 100 Tyr Val Leu Gly Ile 105 Lys Leu Gly Gly Gly Ala Gly 110 Ala Val Pro Pro Leu Thr Lys 120 Arg Ala Lys Pro 125 WO 00/22092 PCT/NZ99/00169 Leu Gly Ala Asn Tyr Arg Leu Ile Asp Ile Pro Val Ser Asn Cys Ile 130 135 140 Asn Ser Asn Ile Ser Lys Ile 145 150 <210> 31 <211> 72 <212> PRT <213> Eucalyptus grandis <400> 31 Ala Pro Ala Leu Ala Ser Gly Ala Ala Ala Phe Lys Ser Val Arg Arg 1 5 10 Ala Pro Ala Val Val Ser Pro Arg Ala Val Ser Asp Ser Arg Asn Ser 25 Gin Thr Cys Leu Asp Pro Asp Ala Ser Arg Ser Val Leu Gly Ile Ile 40 Leu Gly Gly Gly Ala Gly Thr Arg Leu Tyr Pro Leu Thr Lys Lys Arg 55 Ala Lys Pro Ala Val Pro Leu Gly <210> 32 <211> 124 <212> PRT <213> Eucalyptus grandis <400> 32 Leu Lys Asp Ala Ile Ile Ser His Gly Cys Phe Leu Arg Glu Cys Arg 1 5 10 Val Glu Arg Ser Ile Val Gly Glu Arg Ser Arg Leu Asp Ser Gly Val 25 Glu Leu Lys Asp Thr Val Met Met Gly Ala Asp Tyr Tyr Gin Thr Glu 40 Ser Glu Ile Ala Ser Leu Leu Ala Glu Gly Lys Val Pro Ile Gly Ile 55 Gly Lys Asn Thr Lys Ile Arg Asn Cys Ile Ile Asp Lys Asn Ala Lys 70 75 Ile Gly Lys Asp Val Ala Ile Val Asn Lys Asp Gly Val Glu Glu Ala 90 Asp Arg Pro Gly Asp Gly Phe Tyr Ile Arg Leu Gly Ile Thr Val Ile 100 105 110 Leu Glu Lys Ala Thr Ile Glu Asp Gly Thr Val Ile 115 120 <210> 33 <211> 67 <212> PRT <213> Pinus radiata <400> 33 Ile Arg Ile Val Leu Gin Gly Phe Asn Trp Glu Ser His Arg Ser Gly 1 5 10 Gly Trp Tyr His Lys Leu Ser Gly Lys Ala Ala Glu Ile Ala Ser Lys 25 Gly Phe Thr Ile Val Trp Leu Pro Pro Pro Thr Asp Ser Val Ser Pro 40 WO 00/22092 PCT/NZ99/00169 Glu Gly Tyr Met Pro Arg Asp Leu Tyr Asp Leu Asn Ser Arg Tyr Gly 55 Ser Leu Glu <210> 34 <211> 157 <212> PRT <213> Pinus radiata <400> 34 Asn Gin Asp Ala His Arg Gin Arg Ile Val Asn Trp Ile Asn Ala Thr 1 5 10 Gly Gly Ser Ser Ser Ala Phe Asp Val Thr Thr Lys Gly Ile Leu His 25 Val Ala Leu His Asn Gin Tyr Trp Arg Leu Ile Asp Pro Gin Gly Lys 40 Pro Thr Gly Val Met Gly Trp Trp Pro Ser Arg Ala Val Thr Tyr Leu 55 Glu Asn His Asp Thr Gly Ser Thr Gin Gly His Trp Pro Phe Pro Arg 70 75 Asp Lys Leu Thr Gin Gly Tyr Ala Tyr Ile Leu Thr His Pro Gly Thr 90 Pro Thr Ile Phe Tyr Asp His Phe Tyr Asp Phe Gly Leu His Asp Thr 100 105 110 Ile Thr Glu Leu Ile Asp Ala Arg Thr Arg Ala Gly Ile His Cys Arg 115 120 125 Ser Thr Leu Lys Ile Phe His Ala Asn Asn Glu Gly Tyr Ala Ala Gin 130 135 140 Ile Asp Glu Asn Leu Val Met Lys Leu Gly Gin Phe Asp 145 150 155 <210> <211> 332 <212> PRT <213> Eucalyptus grandis <400> Pro Thr Asp Ser Val Ser Pro Glu Gly Tyr Met Pro Arg Asp Leu Tyr 1 5 10 Asn Leu Asn Ser Arg Tyr Gly Thr Ile Asp Glu Leu Lys Asp Leu Val 25 Lys Lys Phe His Glu Val Asn Ile Arg Val Leu Gly Asp Val Val Leu 40 Asn His Arg Cys Ala Gin Tyr Gin Asn Gin Asn Gly Ile Trp Asn Ile 55 Phe Gly Gly Arg Leu Asn Trp Asp Asp Arg Ala Val Val Ala Asp Asp 70 75 Pro His Phe Gin Gly Arg Gly Asn Lys Ser Ser Gly Asp Asn Phe His 90 Ala Ala Pro Asn Iie Asp His Ser Gin Asp Phe Val Arg Lys Asp Leu 100 105 110 Lys Glu Trp Leu His Trp Leu Arg Ser Glu Ile Gly Tyr Asp Gly Trp 115 120 125 Arg Leu Asp Phe Val Arg Gly Phe Trp Gly Gly Tyr Val Lys Asp Tyr 130 135 140 Leu Asp Ala Ser Glu Pro Tyr Phe Ala Val Gly Glu Tyr Trp Asp Ser WO 00/22092 WO 0022092PCTINZ99/001 69 145 Leu Gin Phe Glu Gly 225 Gly Gly Asp Ile Lys 305 Met Met Gly Phe Lys Asp Leu Vai Trp Lys Gin 145 Ile His Ser Tyr Arg Ile Asp Val 195 Tyr Trp 210 Trp Trp Ser Thr Tyr Ala His Ile 275 Arg Asn 290 Aia Giu Lys Ile <210> <211> <212> <213> <400> Met Giu Val Asp Aia Ile Ala Cys Ala Giu Asn Asp Ser Thr Lys Asn 115 Ala Pro 130 Met Giu rc, Met Arg Pro Val 195 Thr Ile 180 Thr Arg Pro Gin Tyr 260 Phe Arg Arg Gly Tyr 165 Asp Thr Leu Ser Gly 245 Ile Ser As n Asp Pro 325 150 Gly Trp, Lys Ser Arg 230 His Leu His Lys Val 310 Gly Giu Ile Gly Asp 215 Ala Trp Thr Tyr Ile 295 Tyr Tyr Asp Al a 185 Leu Lys Thr Phe Pro 265 Ser Cys Al a Glu His 170 Thr His Gly Phe Pro 250 Gi y Glu Arg Ile Pro 330 155 As n As n Ala Lys Val1 235 Se r Thr Ile Ser Ile 315 Gin Gin Gly Ala Pro 220 Glu Gly Pro Gly Thr 300 Asp Ser Asp Thr Leu 205 Pro Asn Lys Al a Ser 285 Ile Asp Al a Ala 190 Giu Gly His Glu Vai 270 Leu Lys Lys His 175 Gly Arg Val1 Asp Met 255 Phe Ile Ile Val 160 Arg Al a Cys Val1 Th r 240 Gin Tyr Ser Thr Al a 320 36 251
PRT
Eucalyptus grandis 36 Ser Gly Val Pro Leu Giu Lys Giy Giu Val Cys Lys Ala Cys Val 40 Leu Arg Cys Gly Thr 55 Arg Asn Glu Leu Gly 70 Pro Gin Asp Thr Gly Leu Asp Ser Giu Tyr 100 Arg Val Glu Ser Trp 120 Thr LYS Ala Giu Lys 135 Glu Lys Gin Ile Ala 150 Ile Ala Lys Se:L Lys 165 Leu Ile Ile Leu Ala 180 Asp Ser Ala Tyr Pro 200 Cys Asn Thr Cys Gly Glu Ala Val 10 Phe 25 Glu Pro Ser Ile Asn 105 Lys Glu Asp Leu Leu 185 Leu Val Tyr Phe Arg His 90 Asp Asp Al a Al a Ala 170 Phe Trp Al a Giu Glu Ser 75 Ala Glu Lys Gin Ser 155 Pro Phe Leu Asp Cys Ile Ala Thr Arg Thr Lys Val1 140 Giu -fyr His Thr Gin Gin Glu Lys Glu Asn Ser Met Ala His Ile Gly Asn 110 Asn Lys 125 Pro Pro Pro Leu Arg Thr Tyr Arg 190 Ser Ile 205 Phe Pro Cys Gly Met Ala Ser Pro Lys Glu Ser Val 175 Val1 Ile Lys As n Arg Ala Gin Ser Ile Ly s Gin Thr 160 Ile Thr Cys Trp Glu Ile Trp Phe Ala Tyr Ser Trp Val Leu WO 00/22092 WO 0022092PCT/NZ99/00 169 210 Ser Pro Vai 225 Giu Lys Giu 215 220 Asn Arg Ile Thr His Val Asp Arg Leu Ser Ala Ar9 Tyr 230 235 240 Gly Glu Pro Ser Glu Leu Ala Val 245 250 Leu His Val1 Leu Pro Asp Phe Val His 1 Met Arg Val1 Val1 Tyr Trp Ser Lys Pro 145 Giu <210> <211> <212> <213> <400> Pro Arg His Lys Leu Thr Asn Asn Gin Leu Giy Ile Asp Ile Gly Thr 115 <210> <211> <212> <213> <400> Tyr Ile Asp Pro Phe Asp Phe Phe Tyr Val Asp Pro Pro Ser Lys Lys Lys Lys 130 Val Phe Leu Glu 37 12
PR
Pi 37 Le Ly As* Se Gi As] As: Gi' 7
T
nus radiata
U
5 n r y p n 0 y Tyr Gly Pro Al a Lys 70 His Lys Val1 Val1 Ala Phe Val1 55 Leu Asp Gly Phe Ser Met Ile 40 Arg Cys Arg Leu Asn 120 38 534
PRT
Eucalyptus grandis 38 Asn Asn Ser Lys Ala 5 Gin Leu Gly Lys Lys Giy Ile Asp Arg His 40 Asp Ile Asn Met Arg 55 Gly Thr Gly Cys Val 70 Pro Val Ser Gin Lys as Trp Cys Ser Cys Cys 100 Lys Asp Asp Thr Ser 120 Met Thr Gly Lys Asn 135 Asp Leu Giu Asp Ile 150 Lys Ser Ser Leu Met 165 Arg As n 25 Leu Giu Tvr Tyr Asp 105 Arg Ile Leu 25 Asp Gly Phe Arg Cys 105 Leu Tyr Giu Ser Giu Ala Asn Ala Val Ala Gly Gin Arg 10 Cys Arg Leu Asn Pro 90 Gly Leu Leu Glu Gin 170 Ly s Leu Leu Met Gin 75 As n Ile Al a Giu Tyr Tyr Asp Arg 75 Ly s Gly Gly Lys Gly 155 Ly s Arg Val1 Asp Cys Phe Arg Gin Leu Ala Val Al a Giy Gin Met Ser Pro Lys 140 Leu Asn Pro Arg Cys Phe Pro Asn Gly Tyr 125 Met Gin Asn Ile Ala Thr Arg Val1 125 Lys Giu Phe Gly Vai Asp Leu Gin Thr Pro 110 Gly Cy s Phe Arg Gin Leu Cys Lys 110 His Gly Gly Glu Tyr Ser His Met Arg Val1 Val Tyr Phe Pro Asn Gly Tyr Asp Ser Ala Ser Phe Ly s 175 Gin Al a Tyr Asp Phe Phe Tyr Leu Gin Ile Pro Gly Cys Lys Lys Gly Asp 160 Arg WO 00/22092 WO 0022092PCT/NZ99/00169 Phe Gly His Ile 225 Lys Pro Gin His Arg 305 Le u Phe Leu Gly Gly 385 Val1 Glu Leu Aila Leu 465 Pro Val1 Val1 Pro Gly Leu Vai 210 Gly Met Ala Val1 Cy s 290 Leu Leu Ile Phe Val1 370 Gly Leu Asp Ile Gly 450 Phe Phe Val1 Arg Cys 530 Gin Pro 195 Ile Trp His Phe Leu 275 Pro Al a Phe Ile Leu 355 Ser Val1 Ala Ser Pro 435 Val1 Gly Leu Leu Ile 515 Gly Ser Pro Val 180 Giu Gly Thr Ser Cys Gly Ile Tyr Gly 230 Cys Arg Giy 245 Lys Gly Ser 260 Arg Trp Ala Leu Trp Tyr Tyr Ile Asn 310 Tyr Cys Thr 325 Pro Thr Leu 340 Ser Ile Ile Ile Giu Asp Ser Ala His 390 Gly Val. Asp 405 Giu Phe Gly 420 Pro Thr Thr Ser Asp Ala Lys Leu Phe 470 Lys Gly Leu 485 Trp Ser Val 500 Asp Pro Phe Val. Giu Cys Phe Asn Tyr 215 Ser Trp Ala Leu Al a 295 Thr Ile Thr Ala Trp 375 Leu Thr Glu Leu Ile 455 Phe Met Leu Ile Ser 200 Giu Val1 Lys Pro Gly 280 Trp Ile Pro Asn Thr 360 Trp Phe Asn Leu Ile 440 As n Ala Gly Leu Al a 185 Thr Giu Thr Se r Ile 265 Ser Gly Val Al a Phe 345 Gly Arg Al a Phe Tyr 425 Ile Asn Phe Lys Ala 505 Ser Ser Lys Giu Val 250 Asn Val Giy Tyr Val1 330 Ala Val1 Asn Val1 Thr 410 Leu Leu Gly Trp Gin 490 Ser Thr Leu Thr Asp 235 Tyr Leu Giu Lys Pro 315 Cys Ser Leu Giu Phe 395 Val1 Phe Asn Tyr Vali 475 As n Ile Leu Ile Giu 220 Ile Cys Ser Ile Leu 300 Phe Leu Ile Giu Gin 380 Gin Thr Lys Met Gly 460 Ile Arg Phe Met Lys 205 Trp Leu Met Asp Phe 285 Lys Thr Leu Trp Leu 365 Phe Gly Aia Trp Val 445 Ser Val1 Thr Ser Pro 525 Glu 190 Glu Gly Thr Pro Arg 270 Leu Leu Ser Thr Phe 350 Arg Trp Leu Lys Thr 430 Gly Trp His Pro Leu 510 Asp Al a Lys Gly Lys 255 Leu Ser Leu Ile Gly 335 Leu Trp Val1 Leu Ala 415 Thr Val Gly Leu Thr 495 Val Giy Ile Giu Phe 240 Arg His Arg Giu Pro 320 Lys Ala Ser Ile Lys 400 Ala Leu Vai Pro Tyr 480 Ile Trp Leu Pro Lys Gin Thr Gly 520 Val Leu Lys <210> <211> <212> <400> Leu Ala Leu 1 Tyr Trp Leu 39 133
PRT
Pinus racliata 39 Phe Leu Thr Trp Arg Val Lys Asn Pro Asn Thr Asp Ala 5 10 Trp Gly Met Ser Ile Val Cys Giu Leu Trp Phe Ala Phe 25 WO 00/22092 WO 0022092PCT/NZ99/00I 69 Ser Trp Leu Thr Asp Leu Asn Pro Ala Thr Ala Asp Leu Ser Ile Val Ser Asp 115 Ala Ala Ser 130 <210> <211> <212> <213> <400> Leu Leu Val 1 Val Phe Ile Thr Asn Pro Gay Tyr Glu Gly Ser Val Gly Trp Arg Ser Ala Pro Ala Leu Gly 115 Tyr Gly Phe 130 Thr Asn Thr 145 Cys Thr Leu Thr Leu Ser Ile Ile Val 195 <210> <211> <212> <213> <400> Leu Ala Leu 1 Val Asp Val Leu Ala Gly Pro Leu 100 Asp Phe Asp Val1 Arg Glu Ser Gly Ala Gin Leu Ser 70 Lys Ala Gly Pro 40 Asp Leu Pro Tyr Leu 120 Lys Lys Pro Pro Pro 105 Leu Leu Phe Gay Leu 90 Val1 Thr Cys Glu Ile Val Glu Phe Pro Ser Asp Thr Lys Glu Ile Pro Cys Al a Leu Ala 125 Asn Thr Phe As n Al a 110 Met Arg Gly Val Thr Cys Al 1a 206
PRT
Pinus radiata Ser Ala Ala Giu Thr Ser Ile 100 Ser Gly Ile Pro Asn 180 Thr Gin Ser Ser Lys Glu Ile Asn Ile Al a Val1 Ala 165 Leu Gly Arg Thr Leu Thr Asp 70 Tyr Leu Giu Gly Tyr 150 Ile Al a Val1 Ser Leu Ile Glu 55 Ile Cys Ser Ile Arg 135 Pro Cys Ser Leu Phe Met Lys 40 Trp Leu Met Asp Leu 120 Leu Leu Leu Ile Giu 200 Glu Asp 25 Giu Gly Thr Pro Arg 105 Phe Lys Thr Leu T'yr 185 Lys 10 Asn Ala Lys Gly Lys 90 Leu Ser Trp Ser Thr 170 Phe Ser Gly Ile Giu Phe 75 Arg His Arg Leu Leu 155 Gly Met Phe Gly His Val Lys Pro Gin His Giu 140 Pro Glu Leu Gly Val1 Val1 Gly Met Al a Val1 Cys 125 Arg Leu Phe Leu Gin Pro Ile Trp His Phe Leu 110 Pro Leu Ile Ile Phe 190 Ser Glu Ser Ile Cys Lys Arg Leu Ala Al a Ile 175 Ile Ser Ser Cys Tyr Arg Gly Trp Trp Tyr Tyr 160 Pro Ser Leu Arg Trp Ser Gly Vai 41 239
PRT
Eucalyptus grandis 41 Arg His Asp Arg Giu 5 Phe Val Ser Thr Val Gly Glu Pro Ser Gin Leu Ala Pro 10 Asp Pro Leu Lys Giu Pro Pro Leu WO 00/22092 PCT/NZ99/00169 25 Ile Thr Ala Asn Thr Val Leu Ser Ile Leu Ala Val Asp Tyr Pro Val 40 Asp Lys Val Ser Cys Tyr Val Ser Asp Asp Gly Ser Ala Met Leu Thr 55 Phe Glu Ala Leu Ser Glu Thr Ala Glu Phe Ala Arg Lys Trp Val Pro 70 75 Phe Cys Lys Lys His Asn Ile Glu Pro Arg Ala Pro Glu Phe Tyr Phe 90 Ala Gin Lys Ile Asp Tyr Leu Lys Asp Lys Ile Gin Pro Ser Phe Val 100 105 110 Lys Glu Arg Arg Ala Met Lys Arg Glu Tyr Glu Glu Phe Lys Val Arg 115 120 125 Ile Asn Ala Leu Val Ala Lys Ala Gin Lys Met Pro Glu Glu Gly Trp 130 135 140 Thr Met Gin Asp Gly Thr Ala Trp Pro Gly Asn Asn Pro Arg Asp His 145 150 155 160 Pro Gly Met Ile Gin Val Phe Leu Gly His Ser Gly Gly Leu Asp Thr 165 170 175 Asp Gly Asn Glu Leu Pro Arg Leu Val Tyr Val Ser Arg Glu Lys Arg 180 185 190 Pro Gly Phe Gin His His Lys Lys Ala Gly Ala Met Asn Ala Leu Ile 195 200 205 Arg Val Ser Ala Val Leu Thr Asn Gly Ala Tyr Leu Leu Asn Val Asp 210 215 220 Cys Asp His Tyr Phe Asn Asn Ser Lys Ala Leu Lys Glu Ala Met 225 230 235 <210> 42 <211> 253 <212> PRT <213> Eucalyptus grandis <400> 42 Ile Ser Cys Gly Tyr Glu Asp Lys Thr Glu Trp Gly Lys Glu Ile Gly 1 5 10 Trp Ile Tyr Gly Ser Val Thr Glu Asp Ile Leu Thr Gly Phe Lys Met 25 His Ala Arg Gly Trp Ile Ser Ile Tyr Cys Met Pro Pro Arg Pro Ala 40 Phe Lys Gly Ser Ala Pro Ile Asn Leu Ser Asp Arg Leu Asn Gin Val 55 Leu Arg Trp Ala Leu Gly Ser Ile Glu Ile Leu Leu Ser Arg His Cys 70 75 Pro Ile Trp Tyr Gly Tyr Asn Gly Lys Leu Arg Leu Leu Glu Arg Leu 90 Ala Tyr Ile Asn Thr Ile Val Tyr Pro Leu Thr Ser Ile Pro Leu Ile 100 105 110 Ala Tyr Cys Ile Leu Pro Ala Phe Cys Leu Leu Thr Asn Lys Phe Ile 115 120 125 Ile Pro Glu Ile Ser Asn Phe Ala Ser Met Trp Phe Ile Leu Leu Phe 130 135 140 Val Ser Ile Phe Thr Thr Gly Ile Leu Glu Leu Arg Trp Ser Gly Val 145 150 155 160 Ser Ile Glu Asp Trp Trp Arg Asn Glu Gin Phe Trp Val Ile Gly Gly 165 170 175 Thr Ser Ala His Leu Phe Ala Val Phe Gin Gly Leu Leu Lys Val Leu WO 00/22092 WO 0022092PCTINZ99/0OI 69 Ala Asp Ile 225 Gly Gly Ile 195 Gly Asp 210 Pro Pro Val Ser Thr Al a Thr Al a 245 Asn Glu Val1 230 Ile Phe Leu 215 Leu Asn Th r 200 Tyr Ile Ser 185 190 Val Thr Ser Lys Ala Gly Asp Glu 205 Val Phe Lys Trp Thr Ser Leu Leu 220 Val Asn Ile Ile Gly Ile Val Ala 235 240 Gly Tyr Gin Ser Trp 250 Gin Cys Val Tyr Asp Al a Val Gly Gin Leu 145 His Ile Val1 Pro Asp 225 Phe Val1 Pro Cy s Leu 305 Ile <210> 43 <211> 46 <212> PR' <213> Eu <400> 43 Cys Phe Se: Tyr Leu Se: Glu Thr Al Ser Ile Gli Tyr Leu Ly: Met Lys Ar Ala Lys Al Thr Pro Tr] 115 Val Phe Lei 130 Pro Arg Lei His Lys Ly Leu Thr Asi 18' Asn Tyr Se: 195 Gin Val G1' 210 Gly Ile As] Asp Val Asi Gly Thr Gi' 26 Pro Ser Me, 275 Gly Cys Cy.
290 Ser Glu Va Phe Asn Le 9
T
caiyptus grandis r r a a 0 p 0 r p 0 t 5 1
LI
Ile Asp Asp Pro Asp Asp Gin Pro Gly Val Ala 165 Ala Asn Arg Arg Met 245 Cys Pro Ser Tyr Gly Leu Asp Phe Arg Lys 70 Tyr Lys Gly Ser Tyr 150 Gly Pro Al a Asn Ser 230 Lys Val1 Asn Cys Arg 310 Glu Ala Gly Ala Ala 55 Ile Glu Al a Asn Ser 135 Val Ala Tyr Val1 Leu 215 Asp Gly Phe Leu Cys 295 Asp Ile Val1 Ala Arg 40 Pro Gin Glu Pro As n 120 Gly Ser G iu Ile Arg 200 Cys Arg Leu Asn P~ro 280 Cys Ser Asp Asp Al a 25 Lys Glu Pro Phe Giu 105 Ser Al a Arg As n Leu 185 Glu Tyr Tyr Asp Arg 265 Lys Pro Ly s As n Tyr Met: Trp Phe Ser Lys 90 Glu Arg His Glu Ala 170 Asn Al a Val1 Ala Gly 250 Gin Pro Se r Arg Tyr Pro Leu Val Tyr Phe 75 Val1 Gly Asp Asp Lys 155 Leu Leu Met Gin Asn 235 Ile Ala Ser Ly s Giu 315 Asp Val Ser Pro Phe Val1 Arg Trp His Ile 140 Arg Val1 Asp Cys Phe 220 Arg Gin Leu Ser Lys 300 Asp Glu Asp Lys Val Ser Phe GJlu Ser Leu Phe Cys Lys Lys Ser Gin Lys Ile Lys Giu Arg Arg Val Asn Ala Leu Ser Met Gin Asp 110 Pro Gly Met Ile 125 Glu Gly Asn Glu Pro Gly Phe Gin 160 Arg Val Ser Ala 175 Cys Asp His Tyr 190 Phe Leu Met Asp 205 Pro Gin Arg Phe Asn Thr Val Phe 240 Gly Pro Val Tyr 255 Tyr Gly Tyr Gly 270 Ser Cys Ser Trp 285 Pro Thr Lys Asp Leu Asn Ala Ala 320 His Giu Arg Ser WO 00/22092 PCT/NZ99/00169 325 330 335 Met Leu Ile Ser Gln Met Ser Phe Glu Lys Thr Phe Gly Leu Ser Thr 340 345 350 Val Phe Ile Glu Ser Thr Leu Leu Ala Asn Gly Gly Val Pro Glu Ser 355 360 365 Ala His Pro Ser Met Leu Ile Lys Glu Ala Ile His Val Ile Ser Cys 370 375 380 Gly Tyr Glu Glu Lys Thr Ala Trp Gly Lys Glu Ile Gly Trp Ile Tyr 385 390 395 400 Gly Ser Val Thr Glu Asp Ile Leu Thr Gly Phe Lys Met His Cys Arg 405 410 415 Gly Trp Arg Ser Val Tyr Cys Met Pro Leu Arg Pro Ala Phe Lys Gly 420 425 430 Ser Ala Pro Ile Asn Leu Ser Asp Arg Leu His Gin Val Leu Arg Trp 435 440 445 Ala Leu Gly Ser Val Glu Ile Phe Leu Ser Arg His Cys Pro Leu Trp 450 455 460 Tyr Gly Phe Gly Gly 465 <210> 44 <211> 805 <212> PRT <213> Eucalyptus grandis <400> 44 Met Ala Asp Arg Met Leu Thr Arg Ser His Ser Leu Arg Glu Arg Leu 1 5 10 Asp Glu Thr Leu Ser Ala His Arg Asn Asp Ile Val Ala Phe Leu Ser 25 Arg Val Glu Ala Lys Gly Lys Gly Ile Leu Gin Arg His Gin Ile Phe 40 Ala Glu Phe Glu Ala Ile Ser Glu Glu Ser Arg Ala Lys Leu Leu Asp 55 Gly Ala Phe Gly Glu Val Leu Lys Ser Thr Gin Glu Ala Ile Val Ser 70 75 Pro Pro Trp Val Ala Leu Ala Val Arg Pro Arg Pro Gly Val Trp Glu 90 His Ile Arg Val Asn Val His Ala Leu Val Leu Glu Gin Leu Glu Val 100 105 110 Ala Glu Tyr Leu His Phe Lys Glu Glu Leu Ala Asp Gly Ser Leu Asn 115 120 125 Gly Asn Phe Val Leu Glu Leu Asp Phe Glu Pro Phe Thr Ala Ser Phe 130 135 140 Pro Arg Pro Thr Leu Ser Lys Ser Ile Gly Asn Gly Val Glu Phe Leu 145 150 155 160 Asn Arg His Leu Ser Ala Lys Leu Phe His Asp Lys Glu Ser Leu His 165 170 175 Pro Leu Leu Glu Phe Leu Gin Val His Cys Tyr Lys Gly Lys Asn Met 180 185 190 Met Val Asn Ala Arg Ile Gin Asn Val Phe Ser Leu Gin His Val Leu 195 200 205 Arg Lys Ala Glu Glu Tyr Leu Thr Ser Leu Lys Pro Glu Thr Pro Tyr 210 215 220 Ser Gin Phe Glu His Lys Phe Gin Glu Ile Gly Leu Glu Arg Gly Trp 225 230 235 240 Gly Asp Thr Ala Glu Arg Val Leu Glu Met Ile Gin Leu Leu Leu Asp WO 00/22092 PCT/NZ99/00169 245 250 255 Leu Leu Glu Ala Pro Asp Pro Cys Thr Leu Glu Lys Phe Leu Asp Arg 260 265 270 Val Pro Met Val Phe Asn Val Val Ile Met Ser Pro His Gly Tyr Phe 275 280 285 Ala Gin Asp Asp Val Leu Gly Tyr Pro Asp Thr Gly Gly Gin Val Val 290 295 300 Tyr Ile Leu Asp Gin Val Arg Ala Leu Glu Glu Glu Met Leu His Arg 305 310 315 320 Ile Lys Gin Gin Gly Leu Asp Ile Thr Pro Arg Ile Leu Ile Ile Thr 325 330 335 Arg Leu Leu Pro Asp Ala Val Gly Thr Thr Cys Gly Gin Arg Leu Glu 340 345 350 Lys Val Phe Gly Thr Glu Tyr Ser His Ile Leu Arg Val Pro Phe Arg 355 360 365 Asn Glu Lys Gly Val Val Arg Lys Trp Ile Ser Arg Phe Glu Val Trp 370 375 380 Pro Tyr Leu Glu Arg Tyr Thr Glu Asp Val Ala Ser Glu Leu Ala Gly 385 390 395 400 Glu Leu Gin Gly Lys Pro Asp Leu Ile Ile Gly Asn Tyr Ser Asp Gly 405 410 415 Asn Ile Val Ala Ser Leu Leu Ala His Lys Leu Gly Val Thr Gin Cys 420 425 430 Thr Ile Ala His Ala Leu Glu Lys Thr Lys Tyr Pro Glu Ser Asp Ile 435 440 445 Tyr Trp Lys Lys Phe Glu Glu Lys Tyr His Phe Ser Cys Gin Phe Thr 450 455 460 Ala Asp Leu Ile Ala Met Asn His Thr Asp Phe Ile Ile Thr Ser Thr 465 470 475 480 Phe Gin Glu Ile Ala Gly Ser Lys Asp Thr Val Gly Gin Tyr Glu Ser 485 490 495 His Met Asn Phe Thr Leu Pro Gly Leu Tyr Arg Val Val His Gly Ile 500 505 510 Asp Val Phe Asp Pro Lys Phe Asn Ile Val Ser Pro Gly Ala Asp Met 515 520 525 Ser Ile Tyr Phe Ala Tyr Thr Glu Gin Glu Arg Arg Leu Lys Ser Phe 530 535 540 His Pro Glu Ile Glu Glu Leu Leu Phe Ser Asp Val Glu Asn Lys Glu 545 550 555 560 His Leu Cys Val Leu Lys Asp Lys Lys Lys Pro Ile Ile Phe Thr Met 565 570 575 Ala Arg Leu Asp Arg Val Lys Asn Leu Thr Gly Leu Val Glu Trp Tyr 580 585 590 Gly Lys Asn Ser Lys Leu Arg Glu Leu Ala Asn Leu Val Val Val Gly 595 600 605 Gly Asp Arg Arg Lys Asp Ser Lys Asp Leu Glu Glu Gin Ser Glu Met 610 615 620 Lys Lys Met Tyr Asp Leu Ile Glu Lys Tyr Lys Leu Asn Gly Gin Phe 625 630 635 640 Arg Trp Ile Ser Ser Gin Met Asn Arg Val Arg Asn Gly Glu Leu Tyr 645 650 655 Arg Tyr Ile Cys Asp Thr Lys Gly Val Phe Val Gin Pro Ala Ile Tyr 660 665 670 Glu Ala Phe Gly Leu Thr Val Val Glu Ala Met Thr Cys Gly Leu Pro 675 680 685 Thr Phe Ala Thr Cys Asn Gly Gly Pro Ala Glu Ile Ile Val His Gly 690 695 700 WO 00/22092 PCT/NZ99/00169 Lys Ser Gly Tyr His Ile Asp Pro Tyr His Gly Asp Gin Ala Ala Glu 705 710 715 720 Leu Leu Val Asp Phe Phe Asn Lys Cys Lys Ile Asp Gin Ser His Trp 725 730 735 Asp Glu Ile Ser Lys Gly Ala Met Gin Arg Ile Glu Glu Lys Tyr Thr 740 745 750 Trp Lys Ile Tyr Ser Glu Arg Leu Leu Asn Leu Thr Ala Val Tyr Gly 755 760 765 Phe Trp Lys His Val Thr Asn Leu Asp Arg Arg Glu Ser Arg Arg Tyr 770 775 780 Leu Glu Met Phe Tyr Ala Leu Lys Tyr Arg Pro Leu Ala Gin Ser Val 785 790 795 800 Pro Pro Ala Val Glu 805 <210> <211> 133 <212> PRT <213> Pinus radiata <400> Ile Lys Gin Gin Gly Leu Asp Ile Thr Pro Gin Ile Ile Val Val Thr 1 5 10 Arg Leu Ile Pro Glu Ala His Gly Thr Thr Cys Asn Gin Arg Ile Glu 25 Lys Val Ser Gly Thr Gin His Ser Leu Ile Leu Arg Val Pro Phe Arg 40 Thr Glu Lys Gly Val Leu Arg Asn Trp Val Ser Arg Phe Asp Val Trp 55 Pro Tyr Leu Glu Arg Phe Ser Glu Asp Val Thr Asn Glu Val Thr Ala 70 75 Glu Leu Lys Gly Gin Pro Asp Leu Ile Ile Gly Asn Tyr Ser Asp Gly 90 Asn Leu Val Ala Ser Leu Ile Ala His Lys Gin Gly Ile Thr Gin Cys 100 105 110 Asn Ile Ala His Ala Leu Glu Lys Thr Lys Tyr Pro Asp Ser Asp Ile 115 120 125 Tyr Trp Lys Asn Phe 130 <210> 46 <211> 158 <212> PRT <213> Pinus radiata <400> 46 His Gly Ile Asp Val Phe Asp Pro Lys Phe Asn Ile Val Ser Pro Gly 1 5 10 Ala Asp Met Gin Ile Tyr Phe Pro Tyr Thr Glu Lys Gin His Arg Leu 25 Thr Thr Leu His Gly Thr Iie Glu Glu Leu Leu Phe Ser Pro Glu Gin 40 Thr Ala Glu His Met Cys Ala Leu Asn Asp Arg Lys Lys Pro Ile Ile 55 Phe Ser Met Ala Arg Leu Asp Arg Val Lys Asn Met Thr Gly Leu Val 70 75 Glu Trp Phe Ala Lys Ser Lys Arg Leu Arg Glu Leu Val Asn Leu Val WO 00/22092 WO 0022092PCT/NZ99/O01 69 Val Val Ala Val Ala Glu 115 Asn Gly Gin 130 Gly Giu Leu 145 Gly 100 Ile Phe Tyr Ile Lys Trp Tyr 150 Pro Ser Lys Ser Lys Asp Arg Glu Glu 105 110 His Arg Leu Val Lys Glu Tyr Asn Leu 120 125 Cys Ala Gin Lys Asn Arg Val Arg Asn 140 Cys Asp Thr Arg Gly Ala Phe 155 Met
I
Asp Arg Ala Gly Ser His Asp Pro Gin Arg Leu Al a Pro As n <2 10> <211> <212> <213> <400> Ala Asp Glu Ala Leu Lys Val. Phe Ala Phe Pro Trp Ile Arg Glu Tyr 115 Asp Ser 130 <210> <211> <212> <213> <400> Glu Ala Pro Gly Glu Gin Asp Gly Phe Thr Gly Val 47 144
PRT
Eucalyptus grandis 47 Arg Val. Leu Asn Arg 5 Leu Phe Ala Asp Arg Ala Lys Giy Lys Gly 40 Glu Ala Ile Pro Glu 55 Gly Lys Val Leu Lys 70 Val Ala Leu Ala Val Val Asn Val His Ala 100 Leu His Phe Lys Giu 120 Glu Pro Leu Thr Ala 135 48
PRT
Eucalyptus grandis 48 Ile Val Ser Pro Pro Val Trp Glu His Ile Leu Giu Val Ala Glu 40 Ser Leu Asn Gly Asn 55 Ala Ser Phe Pro Arg 70 Glu Phe Arg Asn Arg Ser As n 25 Ile Glu Ser Arg Leu 105 Ala Thr Trp, Arg 25 Tyr Phe Pro His His 10 Asp Leu Ser Thr Al a 90 Leu Leu Phe Ser Cy s Gin Arg Gin 75 Arg Leu Val1 Gly Pro Leu Arg Al a Glu Pro Glu Asp Arg 140 Arg Val.
His Arg Ala Gly His Gly 125 Arg Giu Phe Gin Leu Ile Val1 Phe 110 Ser Pro Arg Leu Ile Leu Val1 Trp Gin Leu Phe Leu Ser Leu Asp Ser Glu Val Asn His Val Ala Leu Ala Val. Arg Pro 10 Val Asn Val His Ala Leu Val.
Leu His Phe Lys Glu Glu Leu Val Leu G).u Leu Asp Phe Glu Thr Leu Ser Lys Ser Ile Gly 75 so Leu <210> 49 <211> 247 <212> PRT WO 00/22092 WO 0022092PCT/NZ99/001 69 <213> Pinus radiata Met 1 Ile As n Giu Ile Giu Gly Val1 Ser Ser 145 Gly Met Lys Leu Val1 225 Ile <400> 49 Ala Ala Ala Pro Lys Leu Glu Lys Glu Glu Glu Lys so Val Val Pro Thr Lys Giu Leu Gly Thr 100 Arg Asn Gly 115 Leu Asn Asn 130 Phe Asn Thr Ser Asn Ile Val Ala Glu 180 Giu Ala Trp 195 Asn Ser Gly 210 Phe Ile Ala Leu Phe Ala Pro Ala Val 5 Gin Ala Giu Gly Phe Val Ilie Giu Trp 55 Tyr Asp Thr 70 Leu Leu Asp Thr Met Gly Leu Thr Phe Lys Tyr Asp 135 His Asp Asp 150 Asp Ile His 165 Asp Leu Thr Tyr Pro Pro Lys Leu Asp 215 Asn Ser Asp 230 Leu Val Phe 245 Al a Val Arg 40 Glu Leu Lys cy s Leu 120 Ser Thr Ile Pro Gly 200 Glu Ser Thr 25 Leu Lys Ala Leu Thr 105 Asp Lys Ile Phe Trp 185 His Leu Pro 10 Lys Val1 Ile Al a Val1 90 Gly Leu Val1 Lys As n 170 Pro Giy Leu Al a Leu Ser Lys Leu 75 Val1 Pro Ile Pro Ile 155 Gin Thr Asp Ser Ala Asn Arg Thr Gly Leu Lys Vai Leu 140 Val1 Ser Lys Vai Gin 220 Giu Gin Tyr Pro Giu Lys Ser Lys 125 Val1 Glu Gin Gly Phe 205 Gly Thr Ile 30 Leu Thr Asp Leu Val1 110 Gin Leu Lys Tyr Arg 190 Pro Lys Asp iSe Ser Asp Pro Asn Ile Ile Met Tyr Pro 175 Thr Ala Glu Arg Asp Giy Glu Ser Gly Giu Giu Asn Ser 160 Arg Asp Leu Tyr Asn Leu Gly Ala Ile Val Asp Leu Ser 235 240 Met Gin L-ys Gin Va I Lys Gly <210> <211> <212> <213> <400> Ala Ala Leu Lys Asn Gly His Val P fc0 Tyr Ser Leu Thr Thr 103
PRT
Eucalyptus grandis Ala Ala Thr Leu Ser Ser Ala Vai Ser Gly Phe Ile Asn Leu Val 40 Asp Trp Ser Lys Ile 55 Asp Ser Leu Ala Pro /0 Leu Asp Lys Leu Val Met Gly Cys Thr 100 Pro Asp Ala 10 Gly Gin Ile Arg Tyr Leu Thr Pro Thr Pro Gin Asp 75 Leu Lys Leu 90 Ala Lys Ser Glu Ser Gly Asp Giu Pro Ala Asn Gly Leu Ser Ser Giu Giu Ala Ile Val Ala rhr Gly Leu WO 00/22092 WO 0022092PCTINZ99/0169 Ala His Thr Val1 Phe Val1 Met Gin Ile Ser 145 Phe Glu Lys Asp Glu 225 Giu <210> 51 <211> 25 <212> PR <213> Pi <400> Asn Ser As Leu Val Ly Leu Ala As Gin Leu Le Lys Ser Ii Asn Leu Ly Giu Ile Ii Leu Glu Th 115 Gly Val As: 130 Asp Leu Lei Val Ile Ar' Leu Giy Pr 181 Ser Ile Prc 195 Vai Trp, Ph 210 Ala Lys G1i Asn Lys Va.
3 nus radiata p
S
p
U
e
S
e 0 r n 0 01 0.
Asn Asn Val1 Giu Glu Ala Pro Ala Val Leu Asn 165 Giu Ser Gly Gly Val 245 Leu Lys Lys Ile Lys 70 Ile Asn Al a Pro Val1 150 Pro Phe Ile Ser Ala 230 Ser Gly Asn Gly Ala 55 Phe Lys Pro Gly Arg 135 Gin Ala Lys Ile Gly 215 Thr Ser Al a Glu Gly 40 Gin Lys Arg Lys Ala 120 Ser Ser Arg Lys Asp 200 Val1 Leu Pro Ile Val 10 Tyr Cys 25 Thr Leu Val Pro Ile Phe Leu Val 90 Giu Val 105 Ala Ile Arg Phe Asp Leu Val Asn 170 Val Gly 185 Leu Asp Ile Leu Glu Ile Asp Asp 250 Asp Met Ile Glu Asn 75 Giu Asp Arg Leu Tyr 155 Pro Asn Ser Lys Pro 235 Ile Leu Glu Ser Glu Thr Al a Gly Phe Pro 140 Th r Thr Phe Leu Gly 220 Asp ValI Lys Val Tyr His Asn Asp Val Phe 125 Val1 Val1 Asn Leu Lys 205 Lys Giu Asn Ile Thr Giu Val1 Asn Al a Lys 110 Asp Lys Giu Pro Lys 190 Val Val Ser Leu Pro Gly Gly Leu Leu Val1 Arg Ala Glu Thr 175 Arg Ser Ile Val1 Asn Lys Arg Giu Trp, Lys Leu Ala Thr Gly 160 Ile Phe Gly Ile Ile 240 Met 1e Ser Ile Leu Val1 <210> <211> <212> <213> <400> Ser Thr Giu Arg Ile Ile Arg Gin Glu Ser Leu Asp 52 184
PRT
Pinus radiata 52 Ile Ile Val Pro 5 Leu Arg Lys Ala 20 Gin Ile Leu Gly Ser Tyr Phe Gin 55 Glu Leu Ser Ser 70 Pro Ala Glu Arg Val Phe His 40 Leu Asp Asp Pro Thr Pro Ser Trp Gly Thr Asn Ala Ala Gin Ar9 Glu Asp Leu Leu Lys Ala Val Phe 75 Ile Ser His Gly Glu Glu Ly s Lys Leu Al a Asp Lys Val1 Arg Trp Ile WO 00/22092 WO 0022092PCT/NZ99/00I 69 Lys Arg Asp Phe 145 Pro Giu Met 1 Arg Glu Lys Lys Leu Ala Cys Lys Gly 145 Glu Leu Ile Ala Trp Ser 115 Lys Lys Val I le As Al Se Le As Th 18 n Ala Lys A 0 a Glu Leu Li r Leu Glu G 1I u Leu Val A: 150 p Met His L~ 165 r Giu Gin L' 0 3
T
nus radiata sn lu 35 1la Is <210> 53 <211> 21 <212> PR <213> Pi: <400> 53 Ala Thr Cy Asp Cys GI Giu Lys Il.
Glu Ile Ar Arg Leu G1 Trp Met Hi, Leu Glu Gi1 Thr Arg Se: 115 Arg Tyr G1~ 130 Pro Phe Gil Cys Lys Gi' Tyr Glu Al 181 Arg Ile Ph 195 Cys Asn Ly 210
S
u e, y 0 r a 0 e Ser 5 Giu Ile Giu Lys Giu Trp Ser Arg Lys Val Gly Gin Gin Cys Ile Giu Thr Glu 70 Pro Cys Thr Cys Leu 150 Asp Glu Arg Ala His Ile Tyr 55 Leu Al a Thr Gin Leu 135 Leu Ile Gly Gly Ile Ser 105 Met Val 120 Asp Val Leu Val Ala Ser Arg Val Ser Leu Ala 25 Leu Gly 40 Tyr Ala His Gly Asp Arg Asp Asp 105 Ile Val 120 Asp Asp Leu Ala Asn Lys Arg Cys 185 Giu Cys 200 Leu Leu Glu Ile Ser 110 Arg Gin Ala TPyr His 125 Ala Ala His Thr Ser 140 Ser Ser Tyr Arg Tyr 155 Tyr Glu Ala Lys Lys 170 Ser Ala Ile Arg Gly Asn Glu Gly 160 Leu Ser 175 Cys 10 Cys Ser Met Lys Asp 90 Arg Lys Asp Leu Al a 170 Gly Ser Gly Lys Lys TPyr Leu 75 Al a Ala I le Val Leu 155 Arg Thr Gin Val1 Gly Asn Lys Glu Ile Leu Arg Ile 140 Lys Cys Asp Val As n Leu Glu Glu Lys Ile Ile Giu 125 Cys Ala Asp Giu His 205 Pro Gly Gin Asp Ala Al a Glu 110 Ala Lys His Ala Asp 190 Al a Val Ser Gin Leu Ile Gly Val1 Tyr Thr Arg Lys 175 Thr Ile Giu Asp Arg Cys Val Thr Ile Gin Asn Cys 160 Met Phe Phe <210> <211> <212> <213> <400> Met Ala Thr 1 Ala Glu Gin 54 239
PRT
Eucalyptus grandis 54 Ile Ala Val Pro Pro Ser Val Pro Ser Pro Ala Giu Asp 5 10 Leu Gin Lys Ala Phe Ala Gly Trp Gly Thr Asn Giu Asp WO 00/22092 WO 0022092PCT/NZ99/00I 69 Leu Ile Leu Th r Lys Arg Tyr Phe 145 Pro Glu Val1 Asn Asn 225 Met
I
Ser Leu Ile Leu Ser Lys Arg Tyr Phe 145 Pro Ile Ile Arg Gin s0 Asp Lys Leu Asp Arg Leu Ser Ser 115 Lys Lys 130 Arg Lys Glu Val Lys Ile Thr Thr 195 Asn Giu 210 Asp Glu <210> <211> <2 12> <213> <400> Ser Thr Giu Arg Ile Ile Arg Gin Gin Ser Leu Asp 1byS Trp Ser Ser 115 Lys Lys 130 Arg Lys Giu Val Ser Thr Glu Pro Thr 100 Met Ser Leu As n His 180 Arg Phe Phe Ile Tyr Leu Ala Ser Glu Leu Leu Met 165 Glu Ser Gly Leu Leu Al a Se r 70 Giu Ser Leu Giu Val 150 Th r Lys Lys As n Lys 230 Pro Gi u 55 Ser Arg As n Phe Glu 135 Pro Leu Al a Ala Al a 215 Leu His 40 Thr Asp Asp Trp Met 120 Asp Leu Al a Tyr Gin 200 Ile Leu 25 Arg Tyr Phe Al a Val1 105 Val1 Ile Ala Arg Asn 185 Leu Asn Arg As n Gly Giu Phe 90 Leu Arg Ala Ser Ser 170 His Asn Lys Ser Al a Giu Arg 75 Leu Met Gin Tyr Thr 155 Glu Asp Al a Asp Al a 235 Ala Asp Ser Ser Glu Ala His 140 Phe Ala Giu Thr Leu 220 Ile Gin Leu Val1 As n Ile Tyr 125 Thr Arg Lys Leu Leu 205 Lys Lys Arg Leu Leu Glu Ala 110 His Thr Tyr Ile Ile 190 Asn Ala Cys Lys Lys Leu Al a Cys Ala Gly Glu Leu 175 Arg Tyr Asp Leu Val1 Ala Trp Thr Thr Arg Asp Gly 160 His Ile Tyr Pro 242
PRT
Pinus radiata Ile Ile Val P: 5 Leu Arg Lys A: Giu Ile Leu G~ Thr Tyr Thr G~ Glu Leu Thr A~ 70 Pro Pro Glu A Ser Pro Asn A~ 100 Thr Giu Leu Ti Ser Leu Glu G] 13 Leu Leu Val G] 150 Asp Thr Arg Le la ln -p Lu Ala Pro Ala Pro Ser Pro Val Glu Asp 10 Phe His 40 Leu Asp Asp Arg Ser 120 Asp Leu Al a Giu 25 Arg Tyr Phe Ala Ser 105 Val1 Val Val1 Lys Gly Trp Thr Ala Glu Glu Glu Arg 75 Leu Leu 90 Leu Leu Arg Gin Ala Ser Ser Ser 155 Ser Giu 170 Asn Glu Thr Gin Phe Leu Tyr Ile Tyr 125 Thr Arg Lys Leu Asn Arg Leu Leu Giu Ser 110 His His Tyr Gin Ile Glu Arg Lys Leu Ser Ser Ile Gly Glu Leu 175 Arg Lys Ala Arg Trp, Ile Al a Arg Asp Gly 160 His Ile 165 Giu Ala Ile Lys Asp Lys Ala Phe Gly WO 00/22092 WO 0022092PCT/NZ99/001 69 Ile Lys Ala 225 Leu Thr Thr 195 Asp Asp 210 A r9 Arg Ser 180 185 190 Arg Ser Lys Ala Gin Leu Asn Ala Thr Phe Asn Tyr Tyr 200 205 Tyr Gly His His Ile Asn Lys Asp Leu Lys Glu Trp Glu 215 220 Leu Pro Gly Val Thr Thr Asn Cys Tyr Gin Met His Leu 230 235 240 Met 1 Cys Leu Ile Leu Ser Lys Arg Tyr Phe 145 Asp Glu Leu Lys Lys 225 Arg Arg Giu Ile Lys 305 <210> 56 <211> 31 <212> PR <213> Eu <400> 56 Ser Thr Le Glu Gin Le' Ile Ile Se Arg Gin Th Asp Arg Gl Leu Asp Pr Arg Trp Th Ser Pro G1: 115 Lys Lys Se: 130 Arg Lys Lei Giu Val As~ Lys Ile Se: 18' Ala Thr Ar' 195 Asn Giu Phi 210 Asp Ala Phi Pro Giu Ly Gly Thr As] 261 Val Aso Mel 275 Pro Leu As] 290 Met Leu Lei 6
T
calyptus grandis u
U
r r
U
0 r 0 n r r 0 0 .1 Thr 5 Arg Ile Tyr Leu Ala Ser Gin Leu Leu Met 165 Glu Ser Gly Leu Tyr 245 Glu Lys Arg Ala Val Thr Leu Ala Thr 70 Giu Ser Leu Glu Val 150 Thr Lys Lys As n Thr 230 Ph e Gly Phe Al a Leu 310 Pro Al a Gly Glu Asn Arg Asn Leu Glu 135 Pro Leu Ala Ala Asp 215 Ile Glu Al a lie Ile 295 Ile Gin Phe His 40 Thr Asp Asp Gin Met 120 Asp Leu Ala Tyr Gin 200 Ile Leu Lys Leu Ser 280 Val Gly Pro Al a 25 Arg Tyr Phe Ala Val 105 Ala Val1 Val Lys Gly 185 Val As n Arg Val1 Thr 265 Glu Lys His Leu Pro Pro Val 10 Gly Trp Gly Thr Asn Ala Ala Gln Gly Giu Asp Leu Glu Arg Leu Val 75 Tyr Leu Ala Asn 90 Leu Met Glu Ile Arg Gln Ala Tyr 125 Gly His His Thr 140 Ser Ser Tyr Arg 155 Ala Glu Ala Lys 170 His Glu Asp Leu Asn Ala Thr Leu 205 Lys Asp Leu Lys 220 Ala Thr Val Lys 235 Leu Arg Leu Ala 250 Arg Val Val Ala GUlu il'yr GiLn Arg 285 Asp Thr Thr Gly 300 Val Glu Ala 315 Ala Asn Arg Leu Val Giu Al a 110 His Th r Tyr Ile Ile 190 Asn Thr Cys Ile Thr 270 Arg Asp Asp Glu Lys Ly s Leu Al a Cys Ala Gly Asp Leu 175 Arg His Asp Leu As n 255 Arg Asn Tyr Asp Ly s Leu Ala Trp Thr Thr Arg Asp Gly 160 His Ile Tyr Pro Thr 240 Al a Ser Glu WO 00/22092 WO 0022092PCT/NZ99/00I 69 <210> 57 <211> 418 <212> DNA <213> Eucalyptus grandis <400> 57 ttttgatttt gccattattt atacaatcaa ttctgctgca ttggcgtctg agctgttacc ttcaaatcat gcaaggggat tctgttgggg gatagtcaca tttgacttca cgtgatccga ttccttgata ataaggaggg attcaccaaa aatattggga gacaacgaat caacaaaagg aagggaagcc accacgatac ttacacgtac atatgtcaaa ctcttgcaac tgtaaactgg aattcttcag acctggtgtg aggctcaaca atactcactc gagtacattg tacagtggta at tg9atggca gaagcagtaa atgggatggt c agg ct cac t atccaggaat aaagtgcaaa ccacc t tgg a cgggacagct aagggcagt t ggccatcaag ggcctttccc acctactg 120 180 240 300 360 418 <210> 58 <211> 1396 <212> DNA <213> Eucalyptus grandis <400> 58 atggatagct gcatcccctg tggtacaatt tggcttcctc gatctcaatg catcaaaagg caagatagcc tgggggccgt gat acaggtg aaggagctgt ttcgatttcg ccgaattttg aacccaaacc gccgtcacgg ctttggcggt agtgccgtga ccgtccgata attttctatg ataagggcgc gatttatacg tgggagaaga taggctgcat aaaaaaaaaa tcagcctctt.
ccttgttatt cgctcaagaa caccgtctca cttcgagcta gaatcaaatg ggggaatatg cctttatttg gggacttcaa ctgactggat tactgggtta cggtgggaga aggatgcaca catttgattt tgaaggattc ctttcatcga aggtcatgca atcacttctt ggaaccagat tggccaagat ttgttcctcc agtgatgt tc tgtttgaaat aaaaaa ctggctcttc tcaggggttc cttggtaccg atctgccgct cgggaatcag cctggccgac gtgcatcttc tcgcgatgac taatacgccc gaattggttg tgacccaagc gaactgggac ccgccataag caccacgaag aaacgggatg caatcacgac gggatatgcc tgactggggc taacgagaag cgacgatgga CaaULatcag aggcaaactt aaaaatttac gt tct t ttgg aactgggaat gatttggcca caacaagggt gatgagttga atagtgatta gaaggcggaa actgagttct gacatagacc aagagcgata atcaccaaaa tcgctctctt ctggcggaat gggatcctcc ccgccgggat actggttcca tacatcctca ctgaaggagg agcagtgtgg gtgattatga attgctacat aagaataagc aagcggtctt tcttttacct cttggaagaa atgctggaat acctgcctgg agcatcttat accacaggac cacccgacga ctgatggcat acctcaatcc taggatttga tctacatgga atggacagga gggtcaatgc aggcggccgt tgattggcct cgcaaaaaat cccatcctgg agatcggcaa agatcttggc agattggacc ccggccaaga agcaactgaa atgcaccgta cgccgcctca ggaaggggga tactcatgtg gcggctctat tgatgctttc tgcggaaaaa acgtcttgac gggtaatctt gagggtgcaa tgggtggcga tcggactttg taagaagccc tgcaggcggg tgaaggggag ccagccaagc atggccgttt agtcccatcc gttgacggcg ttccgattct taggtttgaa ttattgtgtg agagcaatta tggaagtaaa 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1396 <210> 59 <211> 1861 <212> DNA <213> Pinus radiata <400> 59 WO 00/22092PC/Z90I6 PCT/NZ99/00169 attgaatttc aaatttgaac tcgaggaaat ggattccaag cgtttctcca gagtcggagt gactaagaag ccctigttagt Ct cggc tt ct ggatgaagga tcagggaaca ggaatttctg agctcacagg agcaacagct gaaacccaaa tgaagagaga agatgcaatg agtcataccg t toggaagat gccaatacca tttgccacca tgtcataaag gggtgcaata gcgaagcttg aaaaagggca ggacaacgtg aactgtaatc acacaaattg atgcagcaat tatgacggaa tcaatgcaac ggggaattca gttctgggcc tctcagggac cggaagaagc cgggcggttt gtgctgggca agagcgaaac aattgcataa ctcaaccgtc tttgtggaag gcagatgctg attctagctg gaaaccaatg tttggtctaa ggagaagagt gccaaggaga ttgaagcttt ggtgccactt attggtacca gacttcagct tcgaagatgc aactgtcaaa atagaggatg ctttcgaata ataattgaca caagaagctg aaagatgctt cctcaaataa tcaaatattt gacattgatg cgtcgatact atacagacga gagaaactgc tctcttcggc atgcgatttt ctgatacttt t tat cc ta gg cagctgttcc atagtaatat atctttcacg tacttgccgc tgagacagta gagatcatct cagatatcac tgaagattaa tgagggcaat tgccttacat tgcgtgaaca cagttggaat tcgaggcttt tttatgatag ttgatgcaga ttcgccactc ccttgctcat aaggtggtgt aaaatgctcg cgagggaaac taattcctag cactactttt ccttagaagg gtttttgtaa tttatgctga aatggctggc cgagttcact ctcttctttg caggaagcag cagtgaactg aggtggagc t tttgggtgcc ctctaagatc ggcctattca tcagcaaagc cttgtggctt ctatcgtatg cgtggcagct tgatgaaggc gagggtggac agctagcatg atttccccaa ggcggtacag ttataatgca atctgctcca tgtgactgac cgttgttgga gggtgctgat cccaat tggc tattggaacc agatgggtac tggtacagtc ttccccacct agagtcctgt ttatgtacga aacogtctat gttatggccg tcgttcaggc tgtgattaca aatattaata acctgtttag gggactcgtc aattataggc tatgttctta agcaacatgg cctgaaaatc tttgaggagc gattatcaga ttacccatgg cgcatcattg actacaattt gggatttatg gc aaa tga tt gcatatttgt aatttgggta atctatactc agtgttattg ctacgttctt tactatgaaa attggaaaag aatgtcaaga ttcataaaga atttaaaact ttagtcgata attttagtgg aattttattg atttttatac caggagttgc cggtatttct gaattttcgc gaagcacagt atccggtcgc tttatccact tgatcgatat cccagttcaa gcagctacaa caaattggtt aacaagtaat aattcattca atgagaaacg aatttgcaga taggcctgga ttgttagtaa t tagaagtga atgatggata ttaccaagaa aatctcgatt gcgagggatg ggatttcaga ctgatgaaga actgccatgt tcatcaacaa gtggtattgt atttggctgt taccgggaaa aaaaataatg gtcattgcct tgttccgagg 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1861 <210> <211> 145 <212> DNA <213> Eucalyptus grandis <400> gggacccagc tctcgactcc gccgatgcct tcaagagcgt gaggagagat ccggacgtcg tgtctcccag agacgtctcc gattcccgga actcgcagac gtgccttaac cccgacgcca gccgcagtgt tctcggaatt atact <210> 61 <211> 441 <212> DNA <~213, Eucalyptus grandis <400> 61 gggccccagc tctcgcctcc ggcgcggctg tc gtgtctcc cagaqccgtc tccqattccc ccagccgcag tgttctcgga attatactag tgactaagaa gagagcaaaa cccgccgtcc ttcctgtgag caattgcttg aatagcaacg attctgcatc tctcaaccgt cacctttctc aaaatgaagg ttttgttgaa gttcttgctg ttcagggcac tgccgatgct g ccttcaagag ggaactcgca gaggtggggc cgt tgggagc tatcgaagat gggcttatgc cccagcagag cgtgcggaga gacgtgcctt cgggacccgt aaattacagg ctatgtcctc cagcaacatg cccggagaat gctccggccg 9a0cc9&cy ctctaccccc ctgattgaca acgcagttca ggtgggtaca cctaactggt 120 180 240 300 360 420 WO 00/22092 WO 0022092PCTr/NZ99/0O 169 <210> 62 <211> 460 <212> DNA <213> Eucalyptus grandis <400> 62 gctttttggg tttgagatct gaatgctctc ttcttgatca cttgctttct tggtgagagt cgcctctttg gctcaatcaa tgagagaatc agagggagcc gagagttgaa aaaaagttca gaacaccgtt gcggagacct cccaaagaaa gtggccgcca tCtctctctc Ctcgttggag tgacggcaat acaagagctg tagataacag agaaggcgaa tgactattag ttatattgg tctgcatcaa ttgcacagca ggattctcgc tctcaagaat tgtttgggtt acccggagtg atctccggtg gggaggtgca gattcggagt tcaaggcttc tgtgtggccc gtagataagg aaacaggttg gcttttgctg ttgcatagac ctgggctctt aagttcggag tcaaggccaa gattcttggg ctagaaatct ttattacatc c aaga g ca ga 120 180 240 300 360 420 460 <210> 63 <211> 341 <212> DNA <213> Eucalyptus grandis <400> 63 aaaatattca tcttaacgca tacaactttg ataatggtgt caaactccgg gtgaagctgg ttcatatggg tctttgagga ggtgaccacc tgtaccggat gccgatatca cagtttcatg gtttaattcc tagttttggt aaagaggtgg tgccaagaac gaattacatg tgtcccaatg ttttccctca gatggatttg ttccagggga aagaatgtgg gatttcgtgc gatgacagtc atcgccacct tggaggtcct ctgctgatgc agaatatctt agaagcatat 9 ttcccggaca cgcagcaact tgttaggcaa gattttgtct tgattcaaat 120 180 240 300 341 <210> 64 <211> 394 <212> DNA <213> Eucalyptus grandis <400> 64 gcaatatctg ttgcctgtac aagatagaca gcagaggcca acagctatgc aagtagatac ccatatattg catcaatggg aagtggaggt accctacagc gagcaagatg tccaggctta tccttctatg atgctaattt tcctgtgggg aattgtccaa caccacccta agtttatgcc caatgacttt tattttccgc ggctctcaca gaaagccggg ttttctgaaa ggattgtctc ttcaagacgg ggatctgaaa gattactggg gaag catctgatta agcctaaggg cgcaagaagc agtcct tact tcattccctc aggatatagg tggattggtg tcctgatctg tgcaagatct aaatcttctg ggctgtgatg gactataaag 120 180 240 300 360 394 <210> <211> 453 <212> DNA <213> Eucalyptus grandis <400> gttttcttct ttcttttctt atttatctgg gtctggcttc cgcgagcatg aaggtcggtg gttctggggt gaaaacttga gagcttgaga agtgaaacaa tactccagaa gttgacaaag agcagatccg aagagttttg tctctcagat cgttCgatat ttcttcgctg cgcgacccgt gggttggtgc ggat tggacg agaccatgac cttcaat tat cgttcggttt tg99ag~c Cc ggtccgtttt gccaggagga taataagcaa tgttaaaccr tcttcaagct act gatgacaagt atttgaaggt gtagggatgg ggcattatca tttggagctc aggaattgctt ccagtgttgg tgggatcttt tgagctcgaa attcttgctt attttagtga ggttatgtaa actctgttct aaactccaag 120 180 240 300 360 420 453 WO 00/22092 WO 0022092PCT/NZ99/001 69 <210> 66 <211> 2055 <212> DNA <213> Eucalyptus grandis <400> 66 at cc tggc at agttgcctcg aggctggtgc tgttgaactt gttttctaat tcgatggcat acatgagagg atcggcaggc gtgattgctg agaagaagga gaaagaacta aaggacttga agaagcggtt tgccagaagg gctatgaaga aagatatctt ccaaaagacc ttctgagatg atgcttgggg accctttcac ctgggaaat t tcctatccat actggtggcg tattccaagg aggcagccga taccaccaac ccataaacaa gggtgatcgt ccacgatcgt ggatcgatcc gctgattctg gctt tacgga aatcttcccg aatgtgcaaa aaaaaaaaaa gatccaggtt acttgtatat aatgaatgct ggattgtgac ggatccccaa tgatcgacat gcttgatggg attgtatggg gccttcatgg tgatacgagt cttgaagaag gggttttgat tggacagtca gactaactcc gaaaacagaa gacaggcttc agctttcaag ggctcttggc aggaaaactc ttccattcct cattatcccc catagccact.
taatgaacaa cctcctcaag ggacagtgag cactctaata tggatacgga ccatctctac ggtcctttgg gttcctgccc gcgtcggat t gctgtttctt attgaaatct gtcctggttt aaaaa tatttgggaa gtgtcccgtg ctggttcgag cactacatca cttggaaaga gacagatatg atacaaggac tatgatcctc tgctcttgtt ttgcttgggc aaagggtctg gagctagaaa cctgtattca acttcactta tggggcaaag aagatgcatt ggatcagcac tccgttgaga aaactgcttg ttgcttttct acgctcacta qgcgtgcttg ttctgggtca gtgcttgccg t ttggt gaa c atcttgaaca tcgtggggcc cctttcctca tccgtacttc aagcaaaccg tcattcaaca tctgtctctt cttgattagc tgaacttttt gtgctggagc agaagcgacc tgtcggcagt acaacagtaa agc t ttgc ta ctaataggaa cagtgtatgt cagtgtccca gctgcggtgg ctgttcatgc gacctgtctt aatcatcgct ttgcctccac ttaaggaagc agattggatg gtagaggatq ctataaatct ttttcctcag agaggcttgc actgtacaat actttgcgag aactacggtg ttggtggcgt gagttgatac tctacctttt tggtcggtgt ctctgttcgg aaggtctgat tcgcctctat gtccagttct cgccgtctct acttgggaca ataggttttg gcaatatatt attggacgtg tggttaccag gctaacaaac 99ctatcagg tgttcaattt catagttttc tggaactgga aaagcggcca ttcaaggaag gaagaagaaa tgatctagaa catgtctcag actaatggaa tatccatgtc gatttatggc gaagtctgta gtcagatcga tcgtcattgt ctatatcaac acctgccgtt cat atggt tc gagtggtgtc atctgcacac taacttcact caagtggact cgtcgccggt gaagctcttc gggaaaacag tttctcattg caaaccgtgt ccgacccgat tattgtaatg cttgaagagt ctgctcaaga gaaggtaagg caccacaaga gcacccttct gaagctatgt cctcagaggt tttgatatca tgtgtgttca aagatgacat tcaaagtcaa aagatgacag gacattgaag aagaattttg gatggtggct ataagttgtq tccgttacag tattgcatgc ctccatcaag cctttgtggt accattgtct tgccttctca ttggcccttt agcatcgagg ctcttcgctq gttacagcaa accctgctca gtttcggatg ttcgcctttt aacaggacac gtctgggtcc ggggtggagt tagatgtgtc cactagggga gtggaactga ttaagcaaaa 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2055 <210> 67 <211> 669 <212> DNA <213> Pinus radiata <400> 67 gcctaacgag tttcctttgt ttcgtgcacc cacttttgtg aagtgaggat tcttggtcat ctggattctg gaccaagccc actttctaag aggtttcaga tcctttcaaa gaacctccac ttatcccatg ggaaaattgt ttatgctctg ttggaagctt atactacact caataatcg ggattctgat taagatggtg gcaatttacc tgactgttat cttgttatgt cacgttttgc ggaaaagaaa tctcatatgt atttgtcgca gccagttcaa gcctgtggat aaacacagta ttctgacgat aaagatctgg tcactcttat tatcgcttgt gaactaggct cgaacagtct atctttattt ttgtccgctc ggaggatcac attccatttt acagagctta tgtatatccc tctcgtacag tcccaaagag gcactgctga ttgccgtaca ctctgacatt gtgataaata 120 180 240 300 360 420 480 WO 00/22092 WO 0022092PCT/NZ99/00 169 ctccattgaa gacagatgtc cagaggttta cttctcaaat cccagtgctc tggaaaacgt aaatctgtcc ttcatgacgg actggaggca tgtaaataag atgtattttg aattgaaaga tcgaatcaac aacgtcatgg agatgggcag tgtccaccag ataaacagaa tgaacaccaa ggattcaag <210> 68 <211> 403 <212> DNA <213> Pinus radiata <400> 68 caacaacata cgtgtgcttg aacgactttc ctttgtatac tgcacccact tttctgcaat gaggattctt ggccatggat attctggatc aggccctaag tctaagaggt ttcagagcaa ttcaaagaac ctccactgac cttcgccttt tacactggaa aatcggtctc tctgatattt atggtggcca gttaccgcct tgttataaac ggtgattgaa aagaaatcac atatgttatc gtcgcagaac gttgaacgaa gtggatatct acagtattgt gcaagctgct tettatacag gcttgttgta taggcttctc cagtcttccc ttatttgcac ccg gatggagcct agcttattcg tatcccaagt gt a cagc tgg aaacagactt tgctgatcct <210> 69 <211> 3851 <212> DNA <213> Pinus radiata <400> 69 ccttgagcgt gaatcttgtc tttttgaatt gttctcataa acacgttgag gcgagctgtt acgagagacg aagggagtcc aatttaatgt gacgcatgag agcttcctcc caacgtcata atccaagctc cttatggctt acaaatcagg caaatgagcc cactgtcccg taattcgact atgcatatgg ttcttgatca cattaaggta gtactgtag tttcagtaga tgcttacttt gcaagaaatt atctnaagga atgaagaatt aaggatggac gtatgatcca ctcggctagt gtgccatgaa atctggattg catggatgaa tttttttatc tggcagagaa cagaaacgag tggccacgtc cgttgcctgt agaaggaaat acgggtggaa ggagactcag ctatggccga gcagattcct ctacgcagac cgagccgggg tgggaacgtg ccagttggat tgaagattat aaaagtgcca gatagtgctg gctctgggcc gtttcccaag cgaacgagaa tccacLqaaq ctaccctgta tgaatctctc cgacat tgag caaggtgcgc aatgcaagac agtgttcttg atatgtttct tgctttggtt tgatcactac tttctgtata acaggggcga ttgaacttaa ttcgtggtca tgccagattt aatgagtgcg cagtcgtgcc ggtgacgatg caaagaaaca ggtcccgacg gtacttgcaa aaccaattgc agtggaagga tcttggaagg atgacggaag attgatcccg attccrttcaa ggtatttttc acttctatcg tggttgccta ggcgaaccat gagcctccrc gacaatgtgt tctgagacct cctcgcgctc atcaatcggt ggtacgcctt ggtcacagtg cgtgagaaga cgggtttctg attaacaata tggatctgat agctttcatg caatggaagc tccatggaca gtggcgagga ggtttcctgt cgcagtgcaa atgaagaaga ggcagcagat acgaaaattc acggccactc ttgccaaccc tcatcatgga agcgaggcga ggagatatca atatgccaat gcaaaataaa tccgctatcg tttgtgaaat tcagtcgtga caatgcttgc tggttactgc cctgttatgt cagaatttgc ccgaaatcta tcaaagagcg tggttgcaaa ggcctggtaa gcggcctcga gacctggttt ctgtgctcac gcaaggcaat ctgatagaaa caggactttt cagcgccggc tgaggagccg cgtcgggctt ctgtcggccg tactcgttac cgttgatgac caccgaggcg gcagattgct ggttgtgagt tgcaatgctg tccaaacagg tggttataaa atataatggg gaccgatgaa tccataccga tctcctgaat ctggtttgcc aacgtatctt acctgttgac caatacagta ctctgatgac cagaaaatgg tttctctcag ccgcgccatg ggcctctaaa taatacccgt tacagaaggc ccagcatcac caatgctcca cagggaaggc ttcagtgtct tagcttaaat ttggttgccg aagcctttga aacacagacg tgctatgagt aagcgtcaaa atagaacatg atgctccacg cataatccag ggggagattc aagcgtgtgc gatattggtt tcgaaggaaa gggtttgcac gcaaggcagc atggtcattg ccagtgaaga ttgtcatgga gatcgactgt ctctttgtga ttatcaatcc ggagcgtcga gtaccattct aaaattgact aagagagaat gtgcccaagg gaccatcctg aatgagcttc aagaaggccg tttatgctga atgtgcttta 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 WO 00/22092 WO 0022092PCT/NZ99/0OI 69 tgatggatcc gtattgatcg aaggtctgga aagctctata attgtctccc gigcaggaat atgatgacga cttcagcit cagctgaatt aatggggaaa tcaagatgca aagggtctgc gaicagtaga tgaaatggct cactcattgc ctcagatcag ctggtattct agttctgggt aggtactggc agtttgggga ttgtcatcaa agtcatgggg atcctcct qgcaattct gtaaggttaa caagtttcaa aagcttatac cttagtatgt ttgaaatatg t c c agctiaa gtaaggatat aattcatiaa aaaaaaaaaa tcaggttggg caaigaccgt tggaattcaa tgggtatggg ttgttgcggt cccagctccc aagagcattg tgttcaatcc gttgaaggag agagcttgga cactcgaggc tccaatcaat aattttcatg tgaaagattt ctattgcaca tacttttgca gaaaatgagg tattggaggg aggcattgat actgtatgca ccttgtgggg icctctcttg caagggtctc gctggcatct aggcccagat i cit ctggaa tagataacag ggataattgg cttcaaagaa gacacgaagg a cc atigg cc ctcgtgccca a agaaaagtct tacgccaatc gggcctgtat cctcccaaag ccicgiaaga gcctacaatc iigaigagcc acicigaigg gctaiicatg tggaiciatg iggcggicca ctaicagacc agcagacati gcciaiaica ciiccagccg agiciatti iggagiggag giiicigcac acaaaiiica ticaaaigga giggtigttg ggiaagciii aigggcaggc giiiictcic aciaaacaat gagagcaaig agcacigggg aggciiitag iacaagaaaa a ccc ciggtia gicaaciiac taattaggca gitaigtcca gaaacaccgt atgtgggaac gcccaaaacg agtciccgaa iggacgggai aaciagact agaaiggtgg icaicagcig gaicagicac titacigiat gittgaacca gcccaaicig acaccatigi icagiiigci iaaiagcici igagcai iga aiiittitgc cagicactgc ccacactcci gcgtagcaga iciiigcatt agaaccgaac iiiicigggi giggcaicaa agaacaacag aaagtaiiic ittccggagg aagtiiagii gaigccaici cicigciaii gcttttacga aiicccicag aiiciiigai iggaigcaig tcccaagatg gaaaaaiagi cgaggaagga cgagaagaag igiiccgcaa iggaiaigaa agaggacait gcccaaacga ggigttgcgt giaiggctai ciaiccatic cactggcaaa titcaicica agaaiggigg agtiaticaa caaggcaica caiicctcct igcaaicaac ctgggicaii acccaccaic aagaaiigai cigcigait agaaacacii ciatiiiig acaaaiiiga iaaaiagiaa gcagciggat attiataic gtigagait agaiicgatg atcaacaiga itcagaagac gigacctgig agcaagaaaa gtagaaggit iiiggccagt acagcaaaic gacaaaacgg cigaciggat gcagcaiica igggciiigg gggggaggi c accicictic iiigigaicc atiiiigcca cgaaaigaac ggictgctca gaigacggig acaacccigc aaiggaiiic gigcaccigi gtggiiaiii cciiicitga ctitgaiaii cagaggcicc iiiaiaaati icigggcici atgitgaiic tiatigitt cciggaaigi gttgtaaaaa 1980 2040 2100 2160 2220 2280 2340 2400 2460 2520 2580 2640 2700 2760 2820 2880 2940 3000 3060 3120 3180 3240 3300 3360 3420 3480 3540 3600 3660 3720 3780 3840 3851 <210> <211> 736 <212> DNA <213> Pinus radiata <400> aaccataiaa gcgggaciig gggcccaagc ggcigcaacc cgicccigii cggiacaggc gacgactiag ggaaacgcci ggagataaii cgiiiiccac gagtgctg ggcagcatca gccgaagcga aactaiigcc iigcaggttc ccatcaggci cgaaiggaga aigagiacga gicacaaagg aacaagaaii tcgacgciac aigcacittc aigcagctac catggaagga aggatggcat gacagc aticgcacag ctaiaagcgg atccaccctc aciaiicgig gagaaaggai gagiccccgg caacaiggaa iccicgggct cciicciccg igigatigga gagagtggag aiaigaicca gaacagaacg aacgagciia caggaiigcc gccigcaacg gggaaccggi gtigaaggcg agagaicgcc gcccacagia aicaiggatg aaiggatiag aaiiggaaag gacgaggccg acgagaicai iggiagiccc aagtcigcgg agigiggati gcigcccica aigatgaaga aaagcgiagi icgctaaccg gcgacagtii aiccagtcaa cgaagcacga atgatataat ggaggccagg tggacacgai agaiaaaatc ccctgigtgi gigcaagaci agaiggcaig cagicacaga ctcgaiaaat aagtgiicag ggagaaciat iaagaaaagt gatgactgaa <210> 71 <211> 448 <212> DNA WO 00/22092 WO 0022092PCT/NZ99/0I 69 <213> Eucalyptus grandis <400> 71 gcttgaaaga gttagtggaa tcaaggaatt ctgcgtaaat tgccctggat gcagcacatg tgggaactat agtgatggta acagtgcacc atagcacatg gaaaaagttt gatgagaagt gaataatgca gactttatca cgttgggcag tatgagagcc cagaacatag ggatctctcg aaattacagc atctggttgc ctttggagaa accacttttc tcaccagcac acactgcc tcatatctta gtttgatgtg ggagttacaa ttcattattg gacaaaatat atgtcaattt ataccaagag cgagttcctt tggccttatc ggctttcctg gctztataaaa ccggactctg actgctgact atagcaggaa tcagatctga tggagacgtt attttataat tgggagtcac acatatattg tactagccat cgaaaaatac 120 180 240 300 360 420 448 <210> 72 <211> 448 <212> DNA <213> Eucalyptus grandis <400> 72 gaacgattga caaacaatcc ctcttcagct tgataacttg ccacactgcc ttcactcttc tccaaagttc aacattgtct aaagcagaag agactcacag acaaaatgat gagcacatag ggcaaggctg gacaaagtga aaaattgagg gaactagcaa ttctcgatat cgaaagcagg cagggctgta cccctggggc ctttgcatgg gatcattgtc agaatatgac atCttgta cgtttcccag aacgaaaaat cagagttgtt agatatgtgc atcaatagaa tgatcggtcc agggctggtt actgaggaaa accgt tgggc catgggattg atctatttcc aagctgctat aagcccatga gaatgctatg ctagaacttt agtatgagag atgtttttga cctactctga atgatcctga tcttttccat ctaagaattc 120 180 240 300 360 420 448 <210> 73 <211> 184 <212> DNA <213> Eucalyptus grandis <400> 73 ggaagccata gttttgcctc ctttcgtggc tatagcagtt cgtccaaggc ctggtgtctg ggaatatgtc cgtgtgaatg tccatgaact gagtgtagag caactgactg tttcagagta tctgggattc aaggaagaac ttgtggatgg caagtctgaa gacagttttg ttcttgagct tgat <210> 74 <211> 1145 <212> DNA <213> Pinus radiata <400> 74 gctgtgttgg gttctcgaga tagcagcagt atgtcttgag gaccaaggcc ataattacgt agccatcttc tccgtgatca g ccagaggct taaagacaga acagcttaca catcgatcca gcgtgttgag tgtaqcacaq aataggagag gtccacacag tggtgtgtgg agctgaiatat attggagtta cattggcagt tgaaagcatg gatgctgaat ggaaaatctt agaaatggga aaggcaaata gacacgctat gggaagaagg aatgatgaac gaggccatca gaatttgttc L a aLL L gatcttgagc ggagttcaat cagcccttgc gagaggatac agcaagctcc ttggaaaaag tggtttctgc ctgagcaccg tt-ttgcaacc accaaaattt ttattccacc gtgtcaatgt aggaacaact ccttcaatgc ttttgaatcg tggatttcct agagtttgac caaagaacac gatggggaga aaggctgact caatcaacta tcatgaaata ccgagatgg atgggttgtt tgatgaactt t gtogatgga atcctttcct acatttgtcc tcgagcacac caagctacga accatataca caatgcagaa cgttctctga gttgctctgt ttagatggat ctatttggaa cttgctgtac gctgttgaac agtgtcaagg cgcccaacac tccagactat aaataccaag tcatcattgg gagttcgaac cgtgttttgg WO 00/22092 WO 0022092PCT/NZ99/OOI 69 atatgattca ttcttggaag gccaagcaaa aagttcgtgc cacctcaaat aacgaattga ctgaa tttgctcttg gattcctatg tgttttagga cttggagagt tattgtggtg aagaattaag gagatccttc gtctttaatq atgccagaca gaaatgctgc actcggttga tgggacacaa aggcacccga ttgtgattct ctggtggaca tgaaaataaa ttcctgaggc tattcacgga tccatcgaga ttcacctcat ggttgtgtat gcggcaagga ccacgggaca tactgcgggt ttagaaacgt ggttattttg atacttgatc ttggacatta acatgcaatc gcccttcaga 840 900 960 1020 1080 1140 1145 <210> <211> 1169 <212> DNA <213> Pinus radiata <400> aagggaccaa aagttggagc cggcacgaga tggaattgat acatgcagat ttatttccct caattgagga actcttgttc accgtaaaaa gcccattatc gattggtcga atggtttgca ttgctggtga tattgaccca aaatgcatag actagttaaa aaaagaatag agtgcggaac ttgtacagcc tgcactttat gtcttcctac attcgcaacc ccggatttca tattgatccc tcgagaagtg caagacagat gtatctatga aaagtataca tttatgggtt ttggaagtat agatgtttta cagcttaaag aaagtgtcaa tgggattgaa cgtgccgacc ccggcacttc tcgagcacag ctgcgcaag tcttgcagtt cattcagggc tcgcgcgcct gtttttgatc tatacagaaa agtcccgaac ttttctatgg aagagcaaac tcaaagtcca gagtataacc ggtgagcttt gaagcttttg tgtaatgggg taccatggcg cccagtcatt tggcagattt gtatcaaaac tacagaaatc gagaagagta gcccaatagc aacgcccgtc caggtaagt gcaggtcgac ccaagttcaa agcagcat cg agacagctga caagactcga gcttgaggga aagacagaga tgaatgggca accgctacat ggct tactgt gtcctgctga tttctgcaac gggagaaga t atgccgacag ttgaaaggcg tggttaaaac cagaattccg agccagtccc gtggccagcc actagtggat cattgtttct ccttactact acatatgtgt ccgggt taaa gttggttaac agaagtggcc gttcagatgg ctgtgacacc tgttgaagca aattattgtg tgaacgcatt ctctaatggt attaatgacg agagacacgc tgtgccatta ttggttgcac ttcccgcttc acgatagccg ccaaagaatt cctggagcag ttacatggta gctttgaatg aatatgacag cttgtcgtgg gaaattgaaa atatgtgcac aggggtgcct atgacatgtg gatggggtgt gctgactttt ggtctacagc ctgt ctggag cgctatctgg gctgttgaag tcgtgccgct agtgacaacg cgctgcctcg 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1169 <210> 76 <211> 420 <212> DNA <213> Pinus radiata <400> 76 atcaaattta gaaacgtttc acctcatggt tattttggcc tgtgtatata cttgatcaat acaaggattg gacattacgc cgggacaaca tgcaatcaac aCqqqLgccc ttcagaacag gtggccttac ttggaaagat taggaagggt aagcaaatgt gtcgcgcctt ctgagattat gacttgaaaa aaaaaggagt tttcggagga tcctatggtc tttaggaatg ggagaatgaa tgtggtgact aattagtgga cgtgcgcgac tgtaacaaat tttaatgttg cccgacaccg atgctattga cggctgatcc acacaacatt tgggtttctc gaaatagccg tgattctttc gtggacaggt gaataaaaca ctgaggctca cacggattct gatttgatgt tggagctgaa 120 180 240 300 360 420 <210> 77 <21 448 <212> DNA <213> Pinus radiata <400> 77 gttgccacat cctgcaatgt tgagctctta ctttcattga aggtgtttct gttaattcgt ctatctcttt agaagttcct cgcctctgta tttcacaaaa gtttcaggag tgtctgtgtt WO 00/22092 WO 0022092PCT/NZ99/00I 69 gtaattccag gggctaatag ccatataaaa agagcgtgtt atatgtagca ggcataggaa tgtggacgga gcaacagcaa gcaaaggaaa gaggacatgc gaagggaaga gaatgt tgac attgaattct ataacagtta atatggttgc tctctgaaca agattttgca ttgaaccc gttatcagag gatccaccag cattgtagga aattctcctt ttaattgttg gtattggcat tgcagcgctc acccatgcac tgagttcacg ccgcaatgaa atagtttctc tgctttcaag gcctcatcaa ctattagatg gactagaaga 180 240 300 360 420 448 <210> 78 <211> 372 <212> DNA <213> Eucalyptus grandis <400> 78 gcgctctccc acgcactctg cccgtcgtca gcaggggtgg gggaaggttc gaaggtgact tcgaatcccg agcatcaggg cgtctctctt ctctccaggt gctggacgag ctcgagaaca ccccttcagt ga cttccagacc aggattcttg gaaactcggc accgggtcga atgtggctca tcatcagcga ccggataccc ggtttctgct gtgttctgtc ggacactctc ggggaagggg ggacgaaggg acgtgtatat ctgttcgtcg atggcggctc gccgctcaca attctgcagc aagagctctc atacccgtct atcggcttga cgaaactggg ggaacgaact cgcatcattt tcagcgatgg 120 180 240 300 360 372 <210> 79 <211> 1960 <212> DNA <213> Eucalyptus grandis <400> 79 cggccgagat tggttacact cctcctcccg tttcgtactc tcctcctttt ttttatcttc ttctctccac gttcatctcc gcccgccaat ggctgccgct tcaaatcggc cgtctccggc acctcgtgtc tcgctatctc cgcccactga cgaaattgtc cagcgaccaa gagccttttg ctacgatggg ttgtactggt ttgaccttat tgttatccaa tgcttctgat gaactctttt ccaactcaaa tatagatatt aggattttat gccactgcct gtcacggtga tgtgttcgca agggcaagga atatgttttt agatcttaaa tcatttgatg ccttagctga tgtgaagggt aaattgcaca ggtccctgat t.ttCaatac aaacaacttg aagctcttaa gatggagatc agctagaaac tgcagctggt tacctcggtc gaggttcctt atcttta-tac tctggtggat atccttcaat cgaattgggc agtcaattcc cagcattatt gcactggcat tactctcaag aaattccgga tggggtggtg gtcaccactt ggttttccag gaccccgagt atgttggtgt tgttattctc ttcactcatc cacatcacca atcgctgtct gccaccctga ctcggccaaa agtggcgaag gtcccttacg gacaaactcg cccaaatctq attgagaatc aacacccatg catactttta tgcaaaggac tccttgatga gctgccaact acaaacaaga ggtacactca gaacacatca zgggtcaact attcctaatc gcagcaatca ccagtgaaag ggt tt tgt tg cctgagttta gagcttgata ggaaaggtga ctggaaaaca aaggaaacat aatctttaca ccccccgtcg tccctcccca ccttcctcct ccctctcaga gcgcccccga tcagcgagag ctcaacatgt atagcttggc ttgtgctgaa tcatagaagt tgaacactaa atgatacgtt atcagagcca agactggaaa acagtggcaa cagataactt acgagtactg tatcttatga atgagttcaa taaaggcaat caaaggaagt agttctttga caacttcaga aacgaaacaa agaaggttg9 gcctgaaggt ccattgctgc aggaaatcca attgagagta tatttggtta ctagccggcc aaagcccaat cctcctcctc tccggagctc cgctgcgaag tgagaagaat tgactggagc gcccactccc gctcaatggt acgcaatgga atatgggtgc gaagattgtt gtatccccgt agatggatgg acttgatgct gggtgctatt tatggaggtt gggaaaggtt atcaattgag taaaaggctt ggatggaatt caatgctatt tctgcttctt ggctagaaca caatttcttg gtctggtgat aaaacctggg tggccctgag gtatttgatc ggtaaataag acttttatat cgaatctcgc ctcctcagcg ttcttcgcta ctctctcagc ggattcatca aagatccaga caggatcctg ggtttgggaa ttgacatttc aatgttcccc gaaaaatatg ttggttgttg tatcctcctg ttgctctcac gttgacctga actccaaaaa caactcttgg aagttcaaga gtcgaagctc aaagttctgc ggcatcaatg gttcagtccg aatccctucaa agcagattca gtatggtttg gttaagctgg gacctctaga ttgcactgga tggacaattc 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 WO 00/22092 WO 0022092PCT/NZ99/001 69 cgtttgaagc atcttaaccc gaaaagagag tgagaaacgc ttctgctgta taagtacctg gaaccttggg aaggcgagga ttttacaatt ttgcttgttt tgctaaacca gtgaggtcat ttaaatgtgc cgtctgctct ttgaatgcca aaaaaaaaaa 1860 1920 1960 <210> <211> 2045 <212> DNA <213> Pinus radiata <400> gtgatctcgg cgccactggt.
catccgaaat tttcgtgact tgctccgctc caactaaaca tgatcggctg agaggattta.
gcataagtgc gtaacagttt tttctatcac gaccgaaaat gatcggatcc ctaaacttca aaggaagggt ttgtgcgact tgggaaaaaa tcaagacccc ttgggagaag atccatcgga aatggcggtt tgggaacaac aatggcctaa catttctgga gattccaaag ttccgttggt attgtagaaa agtactctgg ccacgcatgg tggcagaaga gcatggtacc cacctggcca gatgaactac tctcacaggg gcaatagttg atctcaaaat gaggtcactc ccaaaacgct agagttcagc ttctagagat attgaaaagt tcaaaatctt aggcttgtgg aggctgatgc ggggtaaagg tgcttcagct gcaattggtg tcaatgttcc ctacttgtgc agtcggatct agagtcaacc ctacaaatcc tttctaaagc gtttcaagtc ggtgatgtgt ggtttggcag cagggggcta cacttgagat cctgatgata ttgtgaacta tgtttgtaga gctagaatac tctggtttta tcttccataa cagtttctat gtaaatgttg caaacacaat aaataaatgg aaaaa tttgggtgag gcacatcttc taaagggagg tatgataatt cgtcttctac ggctgcagca agccgaggtc ggtttctcgc gactgacgag aaccaaggaa catgggttgt cttgattgtg gctgatgaat ctcaaatatt tttgacacca cggagatgtt taaggagtac tttgaatcat tgcagatgta tgcacaggtt caataccaat tcttaaaatg tgaaacggct aagatcacgc ttacactgtg caccattgag cat acc tagc tggagtcatc acctgatgaa gcttataagt tgtgtttagt tccttactgg agcgcgaatt tgctttgttg acatcttggt ttcgcataat iggaggagga cttcgctatt gcacgctctg ccagcagttg acgaagctga tacctcagcg atcgtggttc ctcttggaca actgggccca aagcaaatag tcatttaata gacatccaca tggccaacta ttccccgctc gtgttcatag ctggtcaaaa aaaggtggta ccagaggagc aatttatggg gagataattc gcaggtgctg tttctcccag gaggaaggtt ttgggtcctg atcattgatc ctgaagggaa tctgtgatag tttggcagtt gcccttaaag gaggggtttc tttatgcatg agatttttta ttccagagcc cggcggtgga tcgccggaat ggtggtggca caacatagcg atctgtccgc cttcacccgc accagatcag gcgaagaaga cctacgatac agcttgttgt aatccgtcat agtctctgaa cacatgatga tcttcaacca aaggtcgtac ttttgaacag ctaactcaga ataaaaatga c act tat ct c atgttggcga tgaacttgaa ctaatccaaa ctataaggtt tgaaggcaac ttgtcatccg aattcaagaa ttgatagtct aagttataat aaaacaaggt ggcattcttg agtagggtgg ataattt tat aaagcctagg aaaaaaaaaa atactctcat acactacgat ttaacaggaa gacggggata agccgaaact tgataatga9 gaagattgaa gttggcagct gttaaagctt tgaagtacga caacaaatac tacaattaag gagtcaatat agacaaagaa tgggaagctt caacttggga atactgcatg ttatgaaggc attcaaggcg ggcgattaag ggaagtggat ttttgatcgt ttcagattta aaatcctgct ggttggcaac aaaggtgtca tgaagcaaag agtgagcagt atttttgggt aagtttcacc tataactagc atgaagcatc aaaaaaaaaa 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2045 <210> 81 <211> 139 <212> PRT <213> Eucalyptus grandis WO 00/22092 PCT/NZ99/00169 <400> 81 Phe Asp Phe Ala Arg Gly Tyr Ser Pro Lys Tyr Val Lys Glu Tyr lie 1 5 10 Glu Ser Ala Lys Pro Leu Phe Ser Val Gly Glu Tyr Trp Asp Ser Cys 25 Asn Tyr Ser Gly Thr Thr Leu Glu Tyr Asn Gin Asp Ser His Arg Gin 40 Arg lie Val Asn Trp Ile Asp Gly Thr Gly Gin Leu Ser Ala Ala Phe 55 Asp Phe Thr Thr Lys Gly Ile Leu Gin Glu Ala Val Lys Gly Gin Phe 70 75 Trp Arg Leu Arg Asp Pro Lys Gly Lys Pro Pro Gly Val Met Gly Trp 90 Trp Pro Ser Arg Ala Val Thr Phe Leu Asp Asn His Asp Thr Gly Ser 100 105 110 Thr Gin Ala His Trp Pro Phe Pro Ser Asn His Ile Arg Arg Val Thr 115 120 125 Arg Thr Tyr Ser Leu Ile Gin Glu Tyr Leu Leu 130 135 <210> 82 <211> 189 <212> PRT <213> Eucalyptus grandis <400> 82 Leu Phe Trp Leu Phe Val Leu Leu Val Phe Tyr Leu Ala Ala Ser Ala 1 5 10 Ser Pro Ala Leu Leu Phe Gin Gly Phe Asn Trp Glu Ser Trp Lys Lys 25 Glu Gly Gly Trp Tyr Asn Ser Leu Lys Asn Leu Val Pro Asp Leu Ala 40 Asn Ala Gly Ile Thr His Val Trp Leu Pro Pro Pro Ser Gin Ser Ala 55 Ala Gin Gin Gly Tyr Leu Pro Gly Arg Leu Tyr Asp Leu Asn Ala Ser 70 75 Ser Tyr Gly Asn Gin Asp Glu Leu Lys His Leu Ile Asp Ala Phe His 90 Gin Lys Gly Ile Lys Cys Leu Ala Asp Ile Val Ile Asn His Arg Thr 100 105 110 Ala Glu Lys Gin Asp Ser Arg Gly Ile Trp Cys Ile Phe Glu Gly Gly 115 120 125 Thr Pro Asp Asp Arg Leu Asp Trp Gly Pro Ser Leu Ile Cys Arg Asp 130 135 140 Asp Thr Glu Tyr Ser Asp Gly Arg Gly Asn Leu Asp Ser Gly Glu Asp 145 150 155 160 Phe Lys Pro Ala Pro Asp Ile Asp His Leu Asn Pro Arg Val Gin Lys 165 170 175 Glu Leu Ser Asp Trp Met Asn Trp Leu Lys Ser Asp Ile 180 185 <210> 83 <211> 176 <212> PRT <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 Phe Val1 Phe Ser His Pro Val Thr Leu Asn 145 Ser Asp 1 Pro Thr Al a Ala Gin Leu Ala Pro Thr <400> Gly Glu Ala Asn Arg Pro Ser Leu Ala Ile Arg Ala Ala Ser Arg Leu 115 Giy Ala 130 Ser Asn Leu Asn 83 Phe Leu Val1 Cys Phe Val1 Arg 100 Tyr Asn Ile Pro Asn Asn Phe Asp Arg Ser Ser Pro Tyr Ser Ser 165 Thr Val Leu Tyr Lys 70 Asp Val1 Leu Arg Lys 150 Ser Asp Leu Arg Arg 55 Gin Thr Le u Thr Leu 135 Ile Phe Giu Giy Gly 40 Ile Asn Phe Gly Lys 120 Ile Tyr Thr Met Arg 25 Asn Phe Ile Ser Ile 105 Lys Asp Val1 Gly Al a 10 Glu Ser Ala Asn Giu 90 Ile Arg Ile Leu Leu 170 Gly Thr Gin Asp Arg 75 Leu Leu Al a Pro Thr 155 Phe Val Al a Gly Ser Ser Thr Gly Lys Val1 140 Gin Lys Met Giu Leu Lys Thr Cys Gly Pro 125 Ser Phe His Ala Phe Ser Arg Val Leu Gly 110 Al a Asn As n Met Al a Thr Ser Lys Val1 Asp Al a Val1 Cys Ser Gly 175 Gly Ser Ala Lys Ser Pro Gly Pro Ile Al a 160 Ser <210> 84 <211> 47 <212> PRT <213> Eucalyptus grandis <400> 84 Pro Ala Leu Asp Ser Ala Asp 5 Asp Val Val Ser Pro Arg Asp Cys Leu Asn Pro Asp Ala Ser 40 <210> <211> 146 <212> PRT <213> Eucalyptus grandis <400> Pro Ala Leu Ala Ser Gly Ala Pro Ala Val Val Ser Pro Arg Thr Cys Leu Asp Pro Asp Ala 40 Gly Gly Gly Ala Gly Thr Arg 55 Lys Pro Ala Val Pro Leu Gly 70 Val Ser Asn Cys Leu Asn Ser Gin Phe Asn Ser Ala Ser Leu 100 Ala Phe Lys Ser Val Arg Arg Asp 10 Val Ser Asp Ser Arg Asn Ser Gin 25 Arg Ser Val Leu Gly Ile Ile Ala Al a 25 Ser Leu Ala As n As n 105 Ala 10 Val1 Arg Tyr Asn Val1 90 Arg Lys AspD Val1 Leu Arg Lys Leu Ser Ser Leu Thr Leu Ile Ser Val1 Arg Gly Lys Ile Tyr Arg 110 Arg Asn Ile Ly s Aspr Val1 Ala Arg Ser Ile Arg Ile Leu Tyr WO 00/22092 PCT/NZ99/00169 Ala Ser Asn Met Gly Gly Tyr Lys Asn Glu Gly Phe Val Glu Val Leu 115 120 125 Ala Ala Gin Gin Ser Pro Glu Asn Pro Asn Trp Phe Gin Gly Thr Ala 130 135 140 Asp Ala 145 <210> 86 <211> 84 <212> PRT <213> Eucalyptus grandis <400> 86 Glu Leu Thr Ala Met Asp Ser Arg Cys Val Ala Leu Lys Ala Asn Ala 1 5 10 Ser Leu Ala Gin Ser Asn Lys Ser Cys Leu Lys Asn Val Asp Lys Gly 25 Phe Leu Gly Glu Arg Ile Arg Gly Ser Leu Asp Asn Ser Val Trp Val 40 Lys Gin Val Ala Arg Asn Leu Arg Val Glu Lys Lys Phe Lys Lys Ala 55 Lys Pro Gly Val Ala Phe Ala Val Ile Thr Ser Asn Thr Val Ala Glu 70 75 Thr Leu Thr Ile <210> 87 <211> 113 <212> PRT <213> Eucalyptus grandis <400> 87 Lys Ile Phe Ile Leu Thr Gin Phe Asn Ser Phe Ser Leu Asn Arg His 1 5 10 Leu Ser Arg Thr Tyr Asn Phe Asp Asn Gly Val Ser Phe Gly Asp Gly 25 Phe Val Glu Val Leu Ala Ala Thr Gin Thr Pro Gly Glu Ala Gly Lys 40 Arg Trp Phe Gin Gly Thr Ala Asp Ala Val Arg Gin Phe Ile Trp Val 55 Phe Glu Asp Ala Lys Asn Lys Asn Val Glu Asn Ile Leu Ile Leu Ser 70 75 Gly Asp His Leu Tyr Arg Met Asn Tyr Met Asp Phe Val Gin Lys His 90 Ile Asp Ser Asn Ala Asp Ile Thr Val Ser Cys Val Pro Met Asp Asp 100 105 110 Ser <210> 88 <211> 131 <212> PRT <213> Eucalyptus grandis <400> 88 Ala Ile Ser Val Ala Cys Thr Pro Val Gly Glu Ser Arg Ala Ser Asp 1 5 10 WO 00/22092 WO 0022092PCT/NZ99/00I 69 Tyr Gly Leu Val Lys Ile Asp Ser Arg Gly Gin Ile Val Gin Phe Ser 25 Glu Lys Pro Lys Gly Pro Asp Leu Thr Ala Met Gin Val Asp Thr Thr 40 Thr Leu Gly Leu Ser Pro Gin Glu Ala Ala Ary Ser Pro Tyr Ile Aia 55 Ser Met Gly Val Tyr Ala Phe Lys Thr Glu Ser Leu Leu Asn Leu Leu 70 75 Lys Trp Arg Tyr Pro Thr Ala Asn Asp Phe Giy Ser Giu Ile Ile Pro 90 Ser Ala Val Met Giu Gin Asp Vai Gin Aia Tyr Ile Phe Arg Asp Tyr 100 105 110 Trp Glu Asp Ile Gly Thr Ile Lys Ser Phe Tyr Asp Ala Asn Leu Ala 115 120 125 Leu Thr Glu 130 <210> 89 <211> 115 <212> PRT <213> Eucalyptus grandis <400> 89 Arg Leu Ser Ser Lys Phe Ile Trp Val Trp Leu Leu Leu Arg Trp Val 1 5 10 Ary Phe Vai Gly Met Asp Ser Cys Phe Ala Ser Met Lys Val Gly Ala 25 Arg Pro Val Pro Gly Giy Gly Ile Ile Asn Phe Ser Glu Phe Trp Gly 40 Giu Asn Leu Arg Val Gly Ala Asn Lys Gin Phe Giy Ala Arg Leu Cys 55 Lys Ser Leu Ar9 Ser Giu Thr Arg Ile Gly Arg Val Lys Pro Gly Ile 70 75 Ala Tyr Ser Val Leu. Thr Pro Glu Vai Asp Lys Glu Thr Met Thr Leu 90 Gin Ala Pro Val Leu Glu Thr Pro Arg Ala Asp Pro Lys Ser Phe Ala 100 105 110 Ser Ile Ile 115 <210> <211> 600 <212> PRT <213> Eucalyptus grandis <400> Pro Gly Met Ile Gin Val Tyr Leu Gly Ser Ala Gly Ala Leu Asp Val 1 5 10 Glu Gly Lys Glu Leu Pro Arg Leu Val Tyr Val Ser Arg Glu Lys Arg 25 Pro Gly Tyr Gin His His Lys Lys Ala Gly Ala Met Asn Ala Leu Val 40 Arg Val Ser Ala Val Leu Thr Asn Ala Pro Phe Leu Leu Asn Leu Asp 55 Cys Asp His Tyr Ilie Asn Asn Ser Lys Ala Ile Arg Glu Ala Met Cys 70 75 Phe Leu Met Asp Pro Gin Leu Gly Lys Lys Leu Cys Tyr Val Gin Phe WO 00/22092 PCT/NZ99/00169 Pro Asn Gly Tyr 145 Asp Ser Ala Ser Phe 225 Lys Asp Ala Lys Gly 305 Lys Leu Ser Leu Ile 385 Gly Leu Trp Val Leu 465 Ala Thr Val Gly Gln Ile Pro 130 Gly Cys Lys Lys Gly 210 Asp Arg Gly Ile Glu 290 Phe Arg His Arg Glu 370 Pro Lys Ala Ser Ile 450 Lys Ala Leu Val Pro 530 Arg Val 115 Val Tyr Trp Ser Lys 195 Pro Glu Phe Gly His 275 Ile Lys Pro Gin His 355 Arg Leu Phe Leu Gly 435 Gly Val Glu Leu Ala 515 Leu Phe 100 Phe Tyr Asp Pro Lys 180 Lys Val Leu Gly Leu 260 Val Gly Met Ala Val 340 Cys Leu Leu Ile Phe 420 Val Gly Leu Asp Ile 500 Gly Phe Asp Phe Val Pro Ser 165 Lys Lys Phe Glu Gin 245 Pro Ile Trp His Phe 325 Leu Pro Ala Phe Ile 405 Leu Ser Val Ala Ser 485 Pro Val Gly Gly Asp Gly Pro 150 Trp Lys Met Asp Lys 230 Ser Glu Ser Ile Cys 310 Lys Arg Leu Tyr Tyr 390 Pro Ser Ile Ser Gly 470 Glu Pro Ser Lys Ile Ile Thr 135 Val Cys Asp Thr Leu 215 Ser Pro Gly Cys Tyr 295 Arg Gly Trp Trp Ile 375 Cys Thr Ile Glu Ala 455 Val Phe Thr Asp Leu 535 Asp Asn 120 Gly Ser Ser Asp Gly 200 Glu Ser Val Thr Gly 280 Gly Gly Ser Ala Tyr 360 Asn Thr Leu Ile Asp 440 His Asp Gly Thr Ala 520 Phe Arg 105 Met Cys Gin Cys Thr 185 Lys Asp Leu Phe Asn 265 Tyr Ser Trp Ala Leu 345 Ala Thr Ile Thr Ala 425 Trp Leu Thr Glu Leu 505 Ile Phe 90 His Arg Val Lys Cys 170 Ser Asn Ile Met Ile 250 Ser Glu Val Lys Pro 330 Gly Trp Ile Pro Asn 410 Thr Trp Phe Asn Leu 490 Ile Asn Ala Asp Gly Phe Arg 155 Cys Leu Tyr Glu Ser 235 Ala Thr Glu Thr Ser 315 Ile Ser Gly Val Ala 395 Phe Gly Arg Ala Phe 475 Tyr Ile Asn Phe Arg Leu Asn 140 Pro Gly Leu Leu Glu 220 Gin Ser Ser Lys Glu 300 Val Asn Val Gly Tyr 380 Val Ala Val Asn Val 460 Thr Leu Leu Gly Trp 540 Tyr Asp 125 Arg Lys Gly Gly Lys 205 Gly Lys Thr Leu Thr 285 Asp Tyr Leu Glu Lys 365 Pro Cys Ser Leu Glu 445 Phe Val Phe Asn Tyr 525 Val Ala 110 Gly Gin Met Ser Pro 190 Lys Leu Asn Leu Ile 270 Glu Ile Cys Ser Ile 350 Leu Phe Leu Ile Glu 430 Gin Gln Thr Lys Met 510 Gly Ile Asn Ile Ala Thr Arg 175 Val Lys Glu Phe Met 255 Lys Trp Leu Met Asp 335 Phe Lys Thr Leu Trp 415 Leu Phe Gly Ala Trp 495 Val Ser Val Arg Gin Leu Cys 160 Lys His Gly Gly Glu 240 Glu Glu Gly Thr Pro 320 Arg Leu Leu Ser Thr 400 Phe Arg Trp Leu Lys 480 Thr Gly Trp His WO 00/22092 WO 0022092PCT/NZ99/0169 Leu Tyr Pro Phe Leu Lys Gly 545 Thr Val1 Leu Pro Tyr Cys Leu Gin Leu Cys Val Tyr Giu 145 Ser Leu Lys Gly Ile Val Trp Val Lys Pro 595 <210> <211> <212> <213> <400> Asn Glu Arg Ala Tyr Arg Ile Phe Aia Leu Ser Lys Thr Ala Leu Ser 115 Val Ser 130 Ala Ser Ile Giu Giu Asn Met Tyr 195 Ser Val 210 <210> <211> <212> <213> <400> Lys Leu Lys Lys Ile Ile Ser Trp Val1 Arg 580 Cys Leu 565 Ile Gly 550 Trp Asp Val Leu Met Gly Lys Gin Asn Arg Thr Pro 555 560 Val Leu Leu Ala Ser Ile Phe Ser Leu 570 575 Phe Leu Pro Lys Gin Thr Giy Pro Val 585 590 Cys 600 91 222
PRT
Pinus radiata 91 Phe Pro Leu 'I" Tyr Ser Cys T; Leu Leu Tyr I Val Ala Giu L 5.
Arg Trp Trp P2 70 Arg Phe Gin Si Asp Pro Phe L' 100 Ala Leu Ala V Asp Asp Gly G: 1: Arg Phe Ala L' 150 Asp Arg Cys P~ 165 Val Asn Leu S~ 180 Phe Glu Leu L' His Gin Ile As yr hr le eu ro ly 35 Is ro S's sn 15 Thr His Pro 40 Gly Val1 Asn Giu His 120 Ser Ile Giu Phe Asp 200 Arg Thr Phe 25 Ser Phe Gin Leu Pro 105 Tyr Pro Trp Val1 Met 185 Arg Met Leu 10 Cys Giu Ser Arg Pro 90 Pro Pro Leu Ile Tyr 170 Thr Ile As n Giu Al a Asp Tyr Thr 75 Pro Leu Met Thr Pro 155 Phe Asp Asn Thr Lys Ile Ser Ser Val Val1 Thr Gly Phe 140 Phe Ser Trp Asn Lys 220 Lys Ile Trp, Trp Phe Asp Vai Lys 125 Tyr Cys Asn Arg Val1 205 Asp Ser Gly Ser Ile Pro Ile Ile 110 Leu Ala Asp Pro His 190 Met Ser Leu Leu Trp Leu Lys Phe Asn Ser Leu Lys Ser 175 Val1 Glu Leu Ile Ile Asp Arg Ile Thr Cys Leu Tyr 160 Al a Asn Met 2: Se r 1 Giu Al a Asp 92 121
PRT
Pinus radiata 92 Leu Met Giu Pro Asn Asp Phe Pro Leu Tyr Thr Thr Leu 5 10 Ser Leu Leu Tyr Arg Ala Tyr Ser Cys Thr His Phe Ser 25 Gly Leu Ilie Cys Tyvr Arg Leu Leu Tyr Ile Pro Ser Glu 40 Pro Trp Ile Leu Ile Phe Val Aia Glu Leu Gly Phe Ser WO 00/22092PC/Z/0I6 PCT/NZ99/00169 Tyr Thr Pro Leu Leu 1 Ser Ile Val1 Leu T'yr Thr Asp Gin Met 145 Asn Val Le u Gly Gly 225 Lys Tyr Asp Pro Arg 305 Ser Trp Ile Val Phe Pro Vai Asp Ile 100 Thr Vai Ile 115 93 <2i1> 603 <2i2> PRT <213> Pint.
<400> 93 Lys Phe Phe Ala Gly Leu His Gly His Cys Gin Ile Phe Vai Ala Giu Tyr Giu Arg Tyr Lys 100 Giu Giu Asp 115 Gin Arg Asn 130 Ser Tyr Gly Pro Giu Leu Val Ser Gly 180 Ala Asn Pro 195 Ser Gly Arg 210 Phe Gly Asn Ul1u Asn Lys Asn Gly Gly 260 MetL Pro Met 275 Ile Pro Ser 290 Leu Ile Val Leu Asn Phe As n Asp 70 Arg Ile Thr 55 Gin Leu Cys Val1 Ala Leu Arg Trp Trp Pro Vai Glu Arg 75 Ser Lys Arg Phe Gin Ser Lys Leu Pro 90 Thr Ala Asp Pro Phe Lys Glu Pro Pro 105 110 Leu Ser 120 is radiata Giu Val Glu Cys Cys Arg Arg Val1 Arg Arg Pro 165 Giu Al a Ile Val1 Ser 245 Phe Thr Ser Leu Phe Al a Giu Giy Asn 70 Arg Gin Asp Gin Gly 150 Pro le Met Ile Ser 230 Gly Al a Asp Ly s Gly Gly Arg Giu Giy Ser His 25 Pro Lys Pro 40 Giu Asp Val 55 Giu Cys Gly Giu Giy Asn Lys Giy Ser 105 Asp Ile Giu 120 Gin Ile Thr 135 Pro Asp Asp Gin Ile Pro Pro Thr Ser 185 Leu Lys Arg 200 Met Asp Pro 215 Trp Lys Giu Gin Leu Asp Pro Asn Giu 265 Giu Ala Arg 280 Ile Asn Pro 295 Ile Phe Leu Leu Asn Leu Thr Met Glu Ala Asn Leu Gly Phe Gin 90 Pro His Giu Glu Val1 170 Tyr Val1 Asn Arg Met 250 Pro Gin Tyr Arg Arg Asn Leu Pro 75 Ser Arg Glu Al a Asn 155 Leu Tyr His Arg Gly 235 Thr Giu Pro Arg Tvr 315 Ser Asn Glu Thr Leu Asn Thr Val Cys Cys Pro Vai Giu Phe Asn 125 Met Leu 140 Ser Gin Ala Asn Aia Asp Pro Ser 205 Asp Ile 220 Asp Gly Giu Gly Asp Tyr Leu Ser 285 Met Val 300 Ar9 Leu Ile Val Phe Ser Asp Arg Gin Gly 110 Val1 His Ile Gly Asn 190 Ser Giy Tyr Arg Ile 270 Arg Ile Le u Cys Val Val Gly His Gly Giu Pro Cys Cys Asn Asp Asp Giu Thr Giy Arg Ala His 160 His Ser 175 Gin Leu Giu Pro Ser Tyr Lys Ser 240 Tyr Gin 255 Asp Pro Lys Val Val Ile Asn Pro 320 Giu Ile Val Lys Asn Ala Tyr Gly Leu Trp Ala Thr WO 00/22092 PCT/NZ99/00169 325 330 335 Trp Phe Ala Leu Ser Trp Ile Leu Asp Gin Phe Pro Lys Trp Leu Pro 340 345 350 Ile Ser Arg Glu Thr Tyr Leu Asp Arg Leu Ser Leu Arg Tyr Glu Arg 355 360 365 Glu Gly Glu Pro Ser Met Leu Ala Pro Val Asp Leu Phe Val Ser Thr 370 375 380 Val Asp Pro Leu Lys Glu Pro Pro Leu Val Thr Ala Asn Thr Val Leu 385 390 395 400 Ser Ile Leu Ser Val Asp Tyr Pro Val Asp Asn Val Ser Cys Tyr Val 405 410 415 Ser Asp Asp Gly Ala Ser Met Leu Thr Phe Glu Ser Leu Ser Glu Thr 420 425 430 Ser Glu Phe Ala Arg Lys Trp Val Pro Phe Cys Lys Lys Phe Asp Ile 435 440 445 Glu Pro Arg Ala Pro Glu Ile Tyr Phe Ser Gin Lys Ile Asp Tyr Leu 450 455 460 Lys Asp Lys Phe Gin Pro Thr Phe Val Lys Glu Arg Arg Ala Met Lys 465 470 475 480 Arg Glu Tyr Glu Glu Phe Lys Val Arg Ile Asn Arg Leu Val Ala Lys 485 490 495 Ala Ser Lys Val Pro Lys Glu Gly Trp Thr Met Gin Asp Gly Thr Pro 500 505 510 Trp Pro Gly Asn Asn Thr Arg Asp His Pro Gly Met Ile Gin Val Phe 515 520 525 Leu Gly His Ser Gly Gly Leu Asp Thr Glu Gly Asn Glu Leu Pro Arg 530 535 540 Leu Val Tyr Val Ser Arg Glu Lys Arg Pro Gly Phe Gin His His Lys 545 550 555 560 Lys Ala Gly Ala Met Asn Ala Leu Val Arg Val Ser Ala Val Leu Thr 565 570 575 Asn Ala Pro Phe Met Leu Asn Leu Asp Cys Asp His Tyr Ile Asn Asn 580 585 590 Ser Lys Ala Ile Arg Glu Gly Met Cys Phe Met 595 600 <210> 94 <211> 245 <212> PRT <213> Pinus radiata <400> 94 Asn His Ile Lys Leu Leu Pro Phe Ala Gin Glu Gin Asn Asp Glu Ile 1 5 10 Met Glu Ala Arg Ala Gly Leu Val Ala Gly Ser Tyr Lys Arg Asn Glu 25 Leu Met Val Val Pro Gly His Asp Gly Pro Lys Pro Ile Arg Leu Ser 40 Thr Leu Gin Asp Cys Gin Val Cys Gly Asp Lys Ile Gly Cys Asn Pro 55 Asn Gly Glu Leu Phe Val Ala Cys Asn Glu Cys Gly Phe Pro Val Cys 70 75 Arg Pro Cys Tyr Glu Tyr Glu Arg Lys Asp Gly Asn Arg Cys Cys Pro 90 Gin Cys Lys Thr Arg Tyr Arg Arg His Lys Gly Ser Pro Arg Val Glu 100 105 110 Gly Asp Asp Glu Glu Asp Gly Met Asp Asp Leu Glu Gin Glu Phe Asn WO 00/22092 PCT/NZ99/00169 115 120 125 Met Glu Arg Asp Arg Gin Ser Val Val Ser His Arg Gly Asn Ala Phe 130 135 140 Asp Ala Thr Pro Arg Ala Ala His Ser Ile Ala Asn Arg Ser Ile Asn 145 150 155 160 Gly Asp Asn Tyr Ala Leu Ser Leu Pro Pro Ile Met Asp Gly Asp Ser 165 170 175 Leu Ser Val Gin Arg Phe Pro His Ala Ala Thr Val Ile Gly Asn Gly 180 185 190 Leu Asp Pro Val Lys Glu Asn Tyr Gly Ser Ala Ala Trp Lys Glu Arg 195 200 205 Val Glu Asn Trp Lys Ala Lys His Asp Lys Lys Ser Gly Ser Ile Lys 210 215 220 Asp Gly Ile Tyr Asp Pro Asp Glu Ala Asp Asp Ile Met Met Thr Glu 225 230 235 240 Ala Glu Ala Arg Gin 245 <210> <211> 149 <212> PRT <213> Eucalyptus grandis <400> Leu Glu Arg Val Ser Gly Thr Glu His Ser His Ile Leu Arg Val Pro 1 5 10 Phe Arg Ser Asp Gin Gly Ile Leu Arg Lys Trp Ile Ser Arg Phe Asp 25 Val Trp Pro Tyr Leu Glu Thr Phe Ala Leu Asp Ala Ala His Glu Ile 40 Thr Ala Glu Leu Gin Gly Phe Pro Asp Phe Ile Ile Gly Asn Tyr Ser 55 Asp Gly Asn Leu Val Ala Ser Leu Leu Ala Tyr Lys Met Gly Val Thr 70 75 Gin Cys Thr Ile Ala His Ala Leu Glu Lys Thr Lys Tyr Pro Asp Ser 90 Asp Ile Tyr Trp Lys Lys Phe Asp Glu Lys Tyr His Phe Ser Cys Gin 100 105 110 Phe Thr Ala Asp Leu Leu Ala Met Asn Asn Ala Asp Phe Ile Ile Thr 115 120 125 Ser Thr Tyr Gin Glu Ile Ala Gly Thr Lys Asn Thr Val Gly Gin Tyr 130 135 140 Glu Ser His Thr Ala 145 <210> 96 <211> 124 <212> PRT <213> Eucalyptus grandis <400> 96 Leu Ala Lys Ala Gly Thr Lys Asn Thr Val Gly Gin Tyr Glu Ser His 1 5 10 Thr Ala Phe Thr Leu Pro Gly Leu Tyr Arg Val Val His Gly Ile Asp 25 Val Phe Asp Pro Lys Phe Asn Ile Val Ser Pro Gly Ala Asp Met Cys 40 WO 00/22092 WO 0022092PCTINZ99/00I 69 Ile Gly Ile Arg Lys Giu
I
Pro Glu Asp Tyr Phe s0 Ser Ile Gly Ser Leu Asp Asn Ser 115 <210> <211> <212> <213> <400> Ala Ile Gly Val Gin Leu Gly Lys Pro Giu Leu Lys 100 Lys Ser Leu 70 Asp Lys Arg Lys Tyr Ser Met Leu 120 97 61
PRT
Eucalyptus grandis 97 Val Leu Pro Pro Phe 5 Trp Glu Tyr Vai Arg Thr Val Ser Glu Tyr 40 Ser Glu Asp Ser Phe 55 Gin Lys Arg Leu Thr Ala Leu His Asp Pro Glu Gin Asn Asp Giu His 75 Lys Pro Met Ile Phe Ser Met Ala Thr Gly Leu Val Giu Cys Tyr Ala 105 110 Ala Asn Leu Val Val Ala Ile Ala Val Arg Pro Arg 10 is Val Asn Val His Giu Leu Ser Vai 25 Leu Gly Phe Lys Giu Giu Leu Val Vai Leu Giu Leu Asp Cys 1r Arg Lys Gly Val Leu Val1 Phe Giu 145 Pro Ser Leu <210> 98 <211> 21 <212> PR <213> Pi: <400> 98 Val Gly Ii' Ser Leu Se 20 Asn Gin Lel Vai Leu Gi: Giu Asn As] Leu Arg Se.
Ala Vai Ar Asp Glu Le 115 Lys Giu Gi: 130 Leu Asp Lei Ser Ser Ii Arg Leu Ph 18 Arg Ala Hi~ 7
T
rius radiata e r La n p r 0 0 5 Asp 5 Ser Ala Pro Giu Thr Pro Al a Leu Glu Gly 165 Arg Lys Pro Arg Ala His His 70 Gin Arg Val1 Val Pro 150 Ser Asp Tyr Lys Glu Leu Giu Gin Giu Pro Giu Asp 135 Phe Gly His Gin Ala Arg Phe 40 Ile Asn Al a Gly Gin 120 Gly Asn Val1 Glu Gly As n Val1 25 Ser Leu Phe Ile Val1 105 Leu Ser Ala Gin Ser 185 Gin Met 10 Giu Arg Asp Arg Ile 90 Trp Ser Val1 Ser Phe 170 Met Arg Ser Thr Val1 Leu Gly Pro Phe Ala Asp 140 Pro As n Pro Met Ala Leu Ala Ala Leu Pro Val1 Giu 125 Asn Ar9 Arg Leu Leu Arg Ser Gin Ala Phe Trp Arg 110 Tyr Tyr Pro Hi,'s Leu 190 Asn Leu Giu Gly Val1 Gly Val1 Val1 Leu Val1 Thr Leu 175 Asp Glu Thr His Lys Ile Asn Val1 As n Giu Leu Gin 160 Se r Phe Arg WO 00/22092 WO 0022092PCT/NZ99/00I 69 195 Ile Gin Ser Le 210 <210> 99 <211> 34 <212> PR <213> Pi: <400> 99 Gly Thr Lys Se 1 Pro Lys Asn Se Asn Ile Val Se Giu Lys Gin Hi, Leu Phe Ser Pr Arg Lys Lys Pr Asn Met Thr G1' Giu Leu Val As 115 Ser Lys Asp Ar' 130 Vai Lys Giu Ty: 145 Lys Asn Arg Va Arg Giy Ala Phi 181 Val Val Glu Al 195 Gly Gly Pro Al 210 Asp Pro Tyr Hi~ 225 Glu Lys Cys Ly Gly Leu Gin Arc 261 Arg Leu Met Th~ 275 Lys Leu Giu Ar~ 290 Leu Lys Tyr Ar 305 Ser Val Asn Gi! Ser Cys Arg Se~ 341 <210> 101 <211> 13~ 200 u Thr Lys Leu Arg Ser 215 8 T1 nus radiata r r r s 0 0 r 1 e 0 a a r r 0 Trp 5 Ala Pro Arg Glu Ile 85 Leu Leu Glu Asn Arg 165 Val Met Glu Gly Thr 245 Ile Leu Arg As n Ile 325 Cys Ser Arg Gly Leu Gln 70 Ile Vai Val Glu Leu 150 Asn Gin Thr Ile Val1 230 Asp Tyr Ser Glu Leu 310 Glu Arg Ser Asp Ala Thr 55 Thr Phe Giu Val Val1 135 Asn Giy Pro Cys Ile 215 Ser Pro Giu Gly Thr 295 Val1 Glu Pro Arg Gly Asp 40 Thr Al a Ser Trp Val1 120 Ala Gly Giu Al a Gly 200 Val1 Ala Ser L.ys Val1 280 Arg Lys Lys Arg Al a Ile 25 Met Leu Giu Met Phe 105 Al a Glu Gin Leu Leu 185 Leu Asp Thr His Tyr 265 Tyr Arg Thr Ser His 345 Cys Asp Gin His His Ala 90 Ala Gly Ile Phe Tyr 170 Tyr Pro Gly Glu Trp 250 Thr Gly Tvr Val1 Thr 330 Phe Arg Val1 Ile Gly Met 75 Arg Lys Asp Glu Arg 155 Arg Glu Thr Val1 Arg 235 Glu Trp Phe Leu Pro 315 Glu Ala Ser Phe Tyr Thr Cys Leu Ser Ile Lys 140 Trp Tyr Ala Phe Ser 220 Ile Lys Gin Trp, Glu 300 Leu Phe Gin Thr Asp Phe Ile Al a Asp Lys Asp 125 Met Ile Ile Phe Ala 205 Gly Ala Ile Ile Lys 285 Met Ala Arg Le u Pro Pro Giu Leu Arg Arg 110 Pro His Cys Cys Gly 190 Thr Phe Asp Ser Tyr 270 Tyr Phe Val1 Trp Val1 Lys Tyr Giu Asn Val1 Leu Ser Arg Al a Asp 175 Leu Cys His Phe Asn 255 Ala Val1 Tyr Giu Leu 335 Asp Phe Thr Leu Asp Lys Arg Lys Leu Gin 160 Thr Thr Asn Ile Phe 240 Gly Asp Ser Ser Glu 320 His WO 00/22092 WO 0022092PCT/NZ99/0OI 69 Ser Val1 Met Al a Ile Gay Ser Asp Giu Ile Asn Val1 Ser Leu Met Glu Ary Asp Ser <212> <213> <400> Asn Leu Ile Leu Pro Asp Leu Glu Thr Pro Thr Thr Arg Ile Trp Val 115 Asp Val 130 <210> <211> <212> <213> <400> Leu Leu Met Val Glu Asp Arg Tyr so Asp Gly <210> <211> <212> <213> <400> Ala Ala Asp Thr Tyr Val Giu Leu Asp Gly
PRT
Pinus radiata 100 Giu Thr Phe Leu Ser Pro His Gly Thr Giy Gly Gin Asn Giu Met Leu 55 Glu Ile Ile Val 70 Cys Asn Gin Arg Leu Arg Val Pro 100 Ser Arg Phe Asp Thr Asn Giu Ile 135 Gly Tyr Vali 40 Leu Val1 Leu Phe Val1 120 Aila Arg Phe 25 Val1 Arg Thr Giu Arg 105 Trp Vali Val 10 Giy Tyr Ile Arg Lys 90 Thr Pro Giu Pro Gin Ile Lys Leu 75 Ile Giu Tyr Leu Met Al a Leu Gin Ile Ser Ly s Leu Val1 Asn Asp Gin Pro Gly Gly Giu 125 Phe Val1 Gin Gly Giu Thr Vali 110 Arg Asn Leu Cys Leu Aila Gin Val1 Phe Val1 Gly Arg Asp His His Arg Se r 101 68
PRT
Pinus radiata 101 Leu Ile Val Giy Ile Gly Ile His Ile Lys Ala Lys Giu 10 Ala Ala Ala Leu Thr His Aia Leu Ser Ser Arg Giu Arg 25 Met Leu Ser Glu His Arg Asn Giu Ile Vai Ser Leu Leu 40 Vai Ala Glu Gly Lys Lys Ile Leu Gin Pro His Gin Leu 55 Leu 102
PRT
Eucalyptus grandis 102 Pro Lys Leu Gly Arg Ile Pro Ser Ile Arg Asp Arg Vai 10 Leu Ala Ala His Arg Asn Giu Leu Vai Ser Leu Leu Ser 25 Ala Gin Gly Lys Gly Ile Leu Gin Pro His His Leu Leu 40 Giu Asn Ile Ile Ser Giu Asp Giu Gly Lys Ser Ser Leu 55 Pro Phe Ser WO 00/22092 PCT/NZ99/00169 Val 1 Leu Leu Asp Leu Ser Leu Lys Ala Leu 145 Thr Asp Asn Leu Phe 225 Leu His Leu Leu Lys 305 Lys Ile Lys Ile Asn 385 Ala <210> 103 <211> 534 <212> PRT <213> Euc <400> 103 Leu Phe Thr Leu Phe Phe Leu Ser Val Pro Glu Leu Ser Ala Pro Gly Leu Gly Val Ser Arg 100 Ile Gin Thr 115 Pro Thr Pro 130 Val Val Leu Gly Pro Lys Leu Ile Val 180 Val Pro Leu 195 Lys Ile Val 210 Asn Gin Ser Pro Cys Lys Gly Asp Val 260 Leu Ser Gin 275 Gly Ala Ile 290 Asn Glu Tyr Gly Gly Thr Ala Gin Val 340 Phe Lys 11e 355 Lys Arg Leu 370 Pro Lys Glu Gly Ala Ala alyptus grandis His Leu 5 Ile Phe Leu Ser Phe Phe Asp Ala 70 Gin Ile Tyr Leu Pro Thr Gin Asp Lys Leu 150 Ser Val 165 Ile Gin Leu Leu Glu Lys Gin Tyr 230 Gly Gin 245 Phe Ala Gly Lys Val Asp Cys Met 310 Leu lie 325 Pro Asp Phe Asn Val Glu Val Asp 390 Ile Lys Pro Pro His Ile Thr Phe 40 Ala Ser 55 Ala Lys Ser Glu Ser Gly Asp Glu 120 Pro Ala 135 Asn Gly Ile Glu Ile Glu Met Asn 200 Tyr Ala 215 Pro Arg Thr Gly Ser Leu Glu Tyr 280 Leu Lys 295 Glu Val Ser Tyr Glu His Thr Asn 360 Ala Gin 375 Gly lle Phe Phe Gin Lys 10 Thr Thr 25 Ile Ser Pro Pro Leu Ser Ser Glu 90 Glu Ala 105 Ile Val Ala Thr Gly Leu Val Arg 170 Asn Leu 185 Ser Phe Asn Ser Leu Val Lys Asp 250 Met Asn 265 Val Phe Ile Leu Thr Pro Glu Gly 330 Ile Asn 345 Asn Leu Ala Leu Lys Val Asp Asn Pro Phe Ile Met Gin 75 Lys Gin Val Lys Gly 155 Asn Asn Asn Asn Val 235 Gly Ser Ala Asn Lys 315 Lys Glu Trp Lys Leu 395 Ala Asn Leu Ala Ala Leu Asn His Pro Ser 140 Thr Gly Thr Thr Ile 220 Glu Trp Gly Ala His 300 Thr Val Phe Val Met 380 Gin Ile Arg Leu Val Ala Lys Gly Val Tyr 125 Leu Thr Leu Lys His 205 Asp Asp Tyr Lys Asn 285 Leu Leu Gin Lys Asn 365 Glu Leu Gly Ile Leu Ser Ala Ser Phe Asp 110 Asp Leu Met Thr Tyr 190 Asp Ile Phe Pro Leu 270 Ser Met Ala Leu Ser 350 Leu Ile Glu Ile Ser Leu Leu Ala Ala Ile Trp Ser Asp Gly Phe 175 Gly Asp His Met Pro 255 Asp Asp Thr Asp Leu 335 Ile Lys Ile Thr Asn Leu Leu Ser Thr Val Asn Ser Leu Lys Cys 160 Leu Cys Thr Thr Pro 240 Gly Ala Asn Asn Val 320 Glu Glu Ala Pro Ala 400 Val WO 00/22092 WO 0022092PCTINZ99/00I 69 Pro Val1 Lys Phe 465 Ile Thr Val His Met
I
Ile Asn Glu Ile Giu Gly Val1 Ser Se r 145 Gly Met Ly s iLeu Val1 225 Ile Arg Ser Gin Ser 435 Ala Arg.
450 Lys Lys Ile Glu Gly Ile Lys Leu 515 Gly Pro 530 <210> <211> <212> <213> <400> Ala Ala Pro Lys Giu Lys Giu Glu Val Val Thr Lys Leu Gly Arg Asn 115 Leu Asn 130 Phe Asn Ser Asn Val Ala Glu Ala 195 Asn Ser 210 Phe Ile Leu Asn Arg 420 Asp Thr Val1 Leu Thr 500 Giu Glu 405 Phe Leu Asn Gly Asp 485 Leu Ile Asp Leu Tyr Pro Asn 470 Ser Ly s Pro Leu Pro Thr Ser 455 Phe Le u Gly Asp Val Leu 440 Asn Leu Lys Lys Gly 520 Lvs 425 Val1 Pro Ser Val1 Val1 505 Val1 410 Ala Asp Ser Arg Ser 490 Thr Val1 Thr Gly Ile Phe 475 Gly Ile Leu Ser Phe Glu 460 Lys Asp Al a Glu Asp Val1 445 Leu Ser Val1 Al a Asn 525 Leu 430 Giu Gly Ile Trp Lys 510 Lys 415 Leu Arg Pro Pro Phe 495 Pro Gi u Leu As n Glu Ser 480 Gly Gly Ile 104 480
PRT
Pinus radiata 104 Ala Pro Ala V 5 Leu Gin Ala G Glu Gly Phe V~ Lys Ile Giu T~ 5! Pro Tyr Asp TI 70 Giu Leu Leu As Thr Thr Met G 100 Gly Leu Thr P1 Asn Lys Tyr As 1: Thr His Asp AE 150 Ile Asp Ile Hi 165 Giu Asp Leu T1 180 Trp Tyr Pro Pi Gly Lys Leu As 2] Ala Asn Ser As 230 His Leu Val L al lu 'p .ir .0 -p -5 'p Is Ala Ser Pro Aia Ala Giu Thr Asp Arg 10 Val Arg 40 Glu Leu Lys Cys Leu 120 Ser Thr Ile Pro Gly 200 Glu Asn As n Thr 25 Leu Lys Ala Leu Thr 105 Asp Lys Ile Phe Trp 185 His Leu Leu Lys Lys Val1 Ile Al a Val1 90 Gly Leu Val1 Lys Asn 170 Pro Gly Leu Gly As n Leu Ser Lys Leu 75 Val Pro Ile Pro Ile 155 Gin Thr Asp Ser Al a 235 Giu Gly Asn Arg Thr Gly Leu Lys Val Leu 140 Val Ser Lys Val1 Gin 220 Ile Tyr Thr Gin Tyr Pro Glu Lys Ser Lys 125 Val1 Glu Gin Gly Phe 205 Gly Val1 Cys Leu Ile Leu Thr Asp Leu Val 110 Gin Leu Ly s Tyr Arg 190 Pro Lys Asp Met Ile Ser Ser Asp Pro Asn Ile Ile Met Tyr Pro 175 Thr Al a Giu Leu Glu 255 Ser Asp Gly Giu Ser Gly Giu Giu Asn Ser 160 Arg Asp Leu Tyr Lys 240 Vai Tyr 245 250 Thr Pro Lys Thr Leu Ala Asp Val Lys Gly WO 00/22092PCNZ9016 PCT/NZ99/00169 265 Ile Glu Gly Arg 275 Val Gly Giu Gin Leu Leu Ala Gin Val Pro 285 Phe 270 Giu Glu His Asn Thr Asn Phe Lys Ser 290 Leu Ile 295 Lys Lys Phe Lys Ile 300 Leu Asn 305 Ala Trp Vai Asn Ala Ile Lys Arg 315 Lys Vai Giu Ala Asp 320 Val Leu Lys Met Glu 325 Leu Ile Pro Asn Pro 330 Gly Giu Val Asp Giy 335 Lys Val Leu Asp Arg Ala 355 Lys Ala Thr Giu Thr Ala Ala 345 Pro Ala Ala Ile Gly Val Asn Val1 360 Leu Arg Ser Arg Arg Phe Phe 350 Leu Pro Val Tyr Thr Val Ser Asp Leu Vai Gin Ser 370 Giu Glu Asp 380 Val1 Gly Phe Val Asn Pro Ala 385 Pro Arg 395 Lys Asn Pro Thr As n 400 Thr Ile Giu Leu 405 Se r Pro Giu Phe Lys Val Giy Asn Phe Leu 415 410 Ile Lys Arg Phe Val Ser Giy 435 Val Ile Ile Lys 420 Asp Ile Pro Ser Ile 425 Ser Asp Leu Asp Val Trp Phe Giy Gly Val Ile 440 Gly Leu 445 Ile Ser Leu Lys 430 Lys Gly Lys Pro Asp Giu Glu Ala Lys Ala Thr Leu 450 Ser Val 465 Glu 460 Asp Ilie Giu Asn Lys 470 Val Ser Ser Pro 475 Asp Ile Vai Asn 480 <210> 105 <211> 573 <212> DNA <213> Eucalyptus grandis <400> 105 ctcactcgat aaagggcctc ccattgttgc aagatctcct tgagagaaga cgtttggacg gaagccaagg tctgaggaga caggaagcga tgggagcaca ctcgaaggcc ccccattcct gtttaagccc cttcatttcg atttagacat agaccctctc gcaaaggcat gcagagcaaa ttgtgtcgcc tccgtgtgaa agaaggggga ggccgagcct.
catttttcac catcctccgt.
tccaattttc ttttggcgtc ggatttcctg ccttcgccgc ggctgatcgc atgctgactc tgctcaccgc aacgatattg cttgcagcgc caccagattt.
gcttcttgat ggggcctttg tccatgggtt gctcttgctg cgtccatgcg ctt ct tgct t tt t tcgttcgttc ccgtttttgg gccatttgaa gaagccacag tggccttcct ttgctgagtt gtgaagt cct ttcgtccaag ttcgtgtata.
ccttcccttt.
ggctcccttg gttctttttc ccttcgcgag ttcaagggtt tgaggccatc caaatccact gccgggcgtg <210> 106 <211> 105 <212> PRT <213> Eucalyptus grandis <400> 106 Met Ala Asp Arg Met Leu Thr Arg Ser His Ser Leu Arg Glu Arg Leu WO 00/22092 WO 0022092PCT/NZ99/0O1 69 Glu. Thr Leu, Val Glu Ala Ala His Arg Asn 25 Ile Ile Val Ala Arg Lys Gly Lys Ala Giu Phe Gly 40 Glu Leu Gin Arg His Lys Phe Leu Ser Gin Ile Phe Leu Leu Asp Giu Ala Ile Gly Ala Ser 55 Leu Glu Ser Arg Ala Glu Phe Gly Giu.
Pro His Val1 Leu.
Lys Ser Thr Ala Ile Val Pro Trp Val Ile Arg Val 100 <210> 107 Al a Asn Ala Val Arg Pro Pro Gly Val Val His Ala <211> <212> <213> 664
DNA
Eucalyptus grandis <400> 107 ggcacgagct ccacttgggt ccggggttcc tcttcgtggc ttaaggaagg ctgatgctga ctcaggacac acaatgatga agaataagaa agcagatgga ttgccaaaag ca ct cttctcgtct atcgccaaga cctgtgcaac ttgtcaagag aagaaaagcg gagaaatgaa agggattcat gactgggaac gaagaaggcc agagaagcaa caaactcgca cgctttctca actcattggg acttgcggag tgcaacttcg tgcttgcgct ttgggaagtc g ctag a ca ca cctatctgga ccgacgaagg attgctgatg ccataccgaa tataaagaag tcgcgagaag aggctgttgg ccatttgcaa gtggcactcc gatcgacaat tcagcagtgt agaatagagt ctgagaaaga cttcagagcc ccgtaataat tgaaagaata attggccaac ggttgatgag ggcttgtgtc atttgaagcg ggcagctcaa ttctacgttg ggagagctgg ggctcaagtt actctcgacc aatgcgattg cgaggatact atgatggaat aaaggcgagg gaatatgaga aactcgatgg ctcaatgatc gatagtgaat aaggacaaaa ccaccagagc gttattccca atcattttgg <210> <211> <212> <213> 108 184
PRT
Eucalyptus grandis <400> 108 Met Glu Ser Met 1 Gly Val Pro Leu Cys 5 Lys Gly Glu. Val Phe As n 10 Val Thr Cys Gly Glu Ala Val Gly Val Asp Phe Ala Ile Lys Ala Cys Asp Ala Glu Giu.
Cys Ala Cys Gin Lys Ala Cys Val1 40 Thr Tyr Glu Ile Lys As n Giu Cys Asn Glu Gly Arg Ser Met Ala Leu. Arg Cys Arg Asn Glu 70 Pro Gin Asp Gly 55 Leu Pro Phe Glu Ala Thr Gly Ser Arg Leu Ser Al a Met Ala Ala Asn Asp Thr Gly Ile Asp His 90 Asp Arg His Ile Ser Ser Val Ser Thr Trp Lys Asn 115 Lys Ala Pro 130 Leu 100 Arg Ser Giu Tyr Giu Thr Gly Val Giu. Ser Trp 120 Lys Asp Lys Lys Asn 125 Pro Asn Pro Ile 110 Lys Lys Lys Pro Glu Gin Thr Lys Ala Giu 135 Glu Ala Gin Val1 140 WO 00/22092 WO 0022092PCT/NZ99/OOI 69 Gin Met Glu Glu Lys Gin Ile Ala Asp Ala Ser Glu Pro Leu Ser Thr 145 150 155 160 Val Ile Pro Ilie Ala Lys Ser Lys Leu Ala Pro Tyr Arg Thr Val Ile 165 170 175 Ile Met Arg Leu Ile Ile Leu Ala 180 <210> 109 <211> 1293 <212> DNA <213> Pinus radiata <400> 109 ctgactctct gaacgcaccc actgccccct aaatgagaag tcggcaaacc taccaatgat gtacttggcg agcctgcacg caagaagtca ggtacctctt agcagaggct cataaggatt aaatgagtt t tactatactg tcttcgtcta taccagggcc ccctctcgat attgattggc aatatctctg gtagttacca.
tataatggcg tgctagtaag ctctctctgt acacgatctt gtagccgatg ctgatcatat tatgccgaga ttcgagaggc aatgaagcga aggtctccgc atggaagagg gggagctcct aagatactcc ttggctacta ggaaatgata agagctacag gccatcaata gaggttgaca cgtgccattg cacgtcgagg tt t ttggt gt acatgcctcc ttggtttatg tgatcgtgaa tttgtctcct ccattccctc actgcgagca ccatattggg cttacggcga tggtggtcct cgaaaagatg agcagttgct acgtcgctca accgtaatga acgagaagat ggagcaaagc t ca a ca agg a taaagtgcct agcgaggaac tgaagtttat tcaaggacac cttgatttac ggtttgaggc ccagttgtca tacttatgag aaaaaaaaaa ccctcctctc aacaatgtcg gctccggaca tcataggaat ggacctcctc ttggtcactt gacttcaagc tatggcaaga ccacacaact tggagatgag ctcagagaag acaggtcaat tttgaaaact gactcgccct agatgaaggg aagtgaggag tactggagac aagtactcat atttctattt gttgtagcta aagtctttga aaa tctcgttttc actctcaccg gccttcgcag gcggcgcaga aaggcattgg gatccggctg aaccaggttc caagcatatc ggagattttc gtgaatatga gcttatggcc gctacgctga gatccaaaag gagaagtatt gctctgacca taccagagga.
tatgaaaaaa gaagctatcc tccttgcttt tgcgaagcaa ttttgatctt gcttcgtcgt tcccgcagcc gatggggaac ggaagctgat acagagaact aacgtgatgc tcatggaaat atgcccgata gtaagttgct ctttggcaaa atgaggatct atcactacaa acgcgttcct t tgaaaaggt gagtagttgc ggaatagcat tgcttctggc tggtggaggc ccaacaacgt atacacttct taatcaagac 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1293 <210> 110 <211> 484 <212> DNA <213> Pinus radiata <400> 110 ggaagctgat tcggcaaacc acagagaact taccaatgat tggagacgcg tccctctttc ggtggtcctt tggtcgcttg gaaaagatgg acttcaagca gcagttgctc atggcaagac cgtcgctcac cacacaactg ccattatgat ggagatgagg cgag tatgccgaga tttgaggtct tctcagatta atccggctga accaggttct aagcatatca gagatt t tcg tgaatatgac cttacggcga gatcttcttt atccatattc acgtgatgcg catggaaata tgctcgatac taagttgctg tttggcaaaa ggacctcctc aattatttgt attccgtatc tacttggcga.
gcctgcacga aagaagtcgc gtacctcttg SC ag aggct a aaggcattgg attcatccca gtcagaggct atgaagcgac ggtctccaca tggaagagga tgagctccta agatactcca <210> 111 <211> 221 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/00 169 <400> 111 cgtacttggc gaatgaagcg acgaaaagat ggacttcaag caaccaggtt ctaatggaaa tagcctgcac gaggtctccg cagcagttgc ttatggcaag acaagcatat catgcccgat acaagaagtc gctggaagag gacgtcggtc accacacaac tggagatttt cgtaagttgc tggtacctct tgtgagctcc taccgttatg atggagatga g <210> 112 <211> 789 <212> DNA <213> Pinus radiata <400> 112 atcgtcttcg gctcctcgcg gagagagaga gaatggcgac gccgagcagc tccaaaaagc atactgcctc acagaaacgc tatggggaag atcttctcaa gtgcttctgt ggaccctgga aagagattga cttcgagcaa gagttattca tggtgaggca atcgcatatc acactactgg cggtatgagg ggcctgaggt gagaagattc acgagaaggc agtaaagctc agcttaatgc aacaaggatc tgaaggctga aagtgcttg atatcaccaa tatcgcggtg tttcgcagga agcgcagcgg agcgcttgac tcctgcggag Ctgggttctc ggcctatcat ggatttccgc gaacatgaca ttacaatcat aaccctcaat tccaaatgat Cttgcttccg ccaccgtcgg tgggggacga aaagtaatcc aaggaactct cgtgatgcat atggaaattg gctcgttata aagctgcttg ttggcgagat gatgagctca tactacaaca gaatttctga cacagagaga ttccgtctcc atgaagatct gacaaacata ctagtgactt tcttgtccaa cttgcacgag agaaatctct ttcctctggc cagaggctaa tcagaattgt atgagtttgg aactgctgag gagagagaga ggctgaggat gatcatatcc tgccgagaca tgagagatct tgaagctacc gtcttcaatg tgaagaagac aagtaccttt gatacttcat tactacaaga gaatgccatc atcagcaatt <210> 113 <211> 704 <212> DNA <213> Pinus radiata <400> 113 gttttgttga tcgatttcag gtgcgaagta accccatctg aagtcaatca agttattttc gactttgaga tctcatggtg gctcgatctt tccctcgaag cttgtaagtt gcaaagaagc g ctact aga t ttttagttac tgtgatttgt aagactctga tacaaatatt aactgtacga aagctgtatt caataaagaa cggctgaact aagacgtggc catatcggta taagtgaatc tttagtaaat attttggtga cgagaagatg acgcctgagg aggacataga agaggatctc cctttgggta gtggaatgca attgatggtg tgcacataca tgaaggtccg tataaccgag caagaattca agttggtcga tcaacaatta aaggcttttg actgcagcac ttgaagcgat ctagatccag aagaatatat aggcaagcat agtggaaact gaagtggata caaaaaagat t cag ctata a cctgcattgc tagtgccagt aagggtgggg aacgcaaagt tagaatctga ctgaacgtga cgcttttaga atcatattcg tccgtaagtt tgcatttggc aat t attgaggcat tgaagatatc tccaataccg cacaaatgag aatccgtcaa gctttcaagt tgcggtcata aatttccagt gtacaaaaag gctggtagca aagttatgaa <210> 114 <211> 316 <212> PRT <213> Pinus radiata <400> 114 Met Ser Thr Leu Thr Val Pro Gin Pro Leu Pro Pro Val Ala Asp Asp 1 5 10 Cys Glu Gin Leu Arg Thr Ala Phe Ala Gly Trp Gly Thr Asn Glu Lys 25 Leu Ile Ile Ser Ile Leu Gly His Arg Asn Ala Ala Gin Arg Lys Leu WO 00/22092 WO 0022092PCT/NZ99/O1 69 Ile Arg Gin Thr Tyr Ala Giu Thr Tyr Gly Glu Asp Leu Leu Lys Ala Leu Ser Lys Arg Tyr Phe 145 Asp Giu Leu Lys Lys 225 Arg Arg Giu Ile Lys 305 Ser 1 Asp Trp Pro Ly s Lys Val1 Asp Leu Arg Ser Lys 130 Arg Glu Lays Ala Asn 210 Asp Pro Gly Val1 Pro 290 Met Arg Giu Asp Pro Trp Thr 100 Pro Gin 115 Lys Ser Lys Leu Val Asn Ile Ser 180 Thr Arg 195 Glu Phe Ala Phe Glu Lys Thr Asp 260 Asp Met 275 Leu Asp Leu Leu Leu Al a Ser Gin Met Leu Met 165 Giu Ser Gly Leu Tyr 245 Glu Lys Arg Ala Thr 70 Giu Ser Leu Giu Val1 150 Thr Lys Lys As n Thr 230 Phe Giy Phe Ala Leu 55 As n Arg Asn Leu Glu 135 Pro Leu Al a Ala Asp 215 Ile Glu Al a Ile Ile 295 Ile Asp Asp Gin Met 120 Asp) Leu Ala Tyr Gin 200 Ile Leu Lys Leu Ser 280 Val Giy Phe Al a Val 105 Ala Val1 Gly Lys Gly 185 Val1 Asn Arg Val Thr 265 Glu Lys His Glu Tyr 90 Leu Arg Ala Ser Al a 170 His Asn Lys Ala Leu 250 Arg Glu Asp Val1 Arg Leu 75 Leu Ala Met Glu Gin Ala His His 140 Ser Tyr 155 Glu Ala Giu Asp Ala Thr Asp Leu 220 Thr Val 235 Arg Leu Val Val Tyr Gin Thr Thr 300 Glu Ala Val Asn Ile Tyr 125 Thr Arg Lys Leu Leu 205 Lys Lys Al a Al a Arg 285 Gly Val1 Glu Ala 110 His Thr Asn Ile Ile 190 As n Thr Cys Ile Thr 270 Arg Asp Leu Ala Cys Al a Gly Asp Leu 175 Arg His Asp Leu Asn 255 Arg Asn Tyr Trp, Thr Thr Arg Asp Gly 160 His Ile Tyr Pro Thr 240 Lys Al a Ser Glu <210> <211> <212> <213> <400> Ile Phe Pro Ala Thr Ser Gin Gin Ser Leu Leu Leu Asn Met 115 I1I
PRT
Pinus radiata 115 Ile Glu Ser Leu Gi u Val1 T hr 100 Pro 5 Arg Asn Leu Giu Pro Leu Tyr Asp Gin Met Asp 70 Leu Ala Arg Al a Val1 Ala 55 Val Val Lys Gin Tyr Leu 40 Arg Al a Ser Ala Arg Leu 25 Met Gin His Ser Glu 105 Val Asn Ile Tyr Thr 75 His Ly s Val1 Giu Al a His Thr Tyr Ile Leu Ala Cys Ala Gly Asp Leu Trp Thr Thr Arg Asp Gly His 110 Ser Lys Arg Tyr Phe Asp Glu Leu Arg Ser Lys Arg Glu WO 00/22092 WO 0022092PCT/NZ99/00J 69 Tyr Leu Arg Gly Ser Met
I
Al a Leu Ile Leu Thr Lys Arg Tyr Phe 145 Pro Giu Val Asn Asn 225 <210> <211> <212> <213> <400> Leu Ala Met Giu Gin Ala His His Ser Tyr <210'> <211> <212> <213> <400)> Ala Thr Giu Gin Ile Ile Arg Gin Asp Lys Leu Asp Arg Leu Ser Ser 115 Lys Lys 130 Arg Lys Glu Val Lys Ile Thr Thr 195 Asn Giu Asp Giu 116 73
PRT
Pinus radiata 116 Asn Glu Ala Thr Ile Ala Cys Thr Tyr His Ala Arg Thr Thr Gly Asp 55 Arg Tyr Asp Gly 117 239
PRT
Pinus radiata 117 Lys Arg Trp Thr Ser Ser Asn Gin Val 10 Arg Ser Pro Gin Gin Leu Leu Met Ala 25 Tyr Lys Lys Ser Leu Glu Glu Asp Vai 40 Phe Arg Lys Leu Leu Val Pro Leu Val Asp Glu Ile Leu Ser Thr Giu Pro Thr 100 Met Ser Leu As n His 180 Arg Phe Phe Al a Gin Ile Tyr Leu Ala Ser Giu Leu Leu Met 165 Glu Ser Gly Leu Val1 Lys Leu Ala Ser 70 Glu Ser Leu Giu Val1 150 Thr Lys Lys As n Lys 230 Pro Al a Pro Giu 55 Ser Arg Asn Phe Glu 135 Pro Leu Ala Ala Ala 215 Leu Pro Ser Val Pro Ser Pro Ala Glu Asp Phe His 40 Thr Asp Asp Trp Met 120 Asp Leu Ala Tyr Gin 200 Ile Leu Ala 25 Arg Tyr Phe Al a Val1 105 Val1 Ile Al a Arg Asn 185 Leu As n Arg 10 Gly Asn Gly Glu Phe 90 Leu Arg Ala Ser Ser 170 His As n Lys Ser Trp Al a Giu Arg 75 Leu Met Gin Tyr Thr 155 Giu Asp Ala Asp Al a 235 Gly Ala Asp Ser Ser Giu Ala His 140 Phe Ala Glu Thr Leu 220 Ile Thr Gin Leu Val1 Asn Ile Tyr 125 Thr Arg Ly s Leu Leu 205 Lys Lys Asn Arg Leu Leu Glu Al a 110 His Thr Tyr Ile Ile 190 Asn Ala Cys Giu Lys Lys Leu Al a Cys Ala Gly Glu Leu 175 Arg Tyr Asp Leu Asp Val Al a Trp Th r Thr Arg Asp Gly 160 His Ile Tyr Pro <210> 118 <211> 184 <212> PRT WO 00/22092 WO 0022092PCT/NZ99/00I 69 <213> Pinus radiata Met 1 Ser Ser <400> 118 Ser Thr Ile Giu Arg Leu Ilie Ile Gin Ile Val Pro Val Pro Pro Thr Pro Ser 5 Arg Giu Asp Lys Ala Phe Giu 25 Arg Trp Giy Thr Ile Leu Giy Ile Arg Gin His 40 Leu Thr Ala Ala Gin Leu Asn Giu Lys Arg Lys Vai Leu Lys Arg Ser Tyr Phe Tyr Giu Giu Glu Leu Val1 Ser Giu Leu Ser 70 Glu Asp Phe Giu Val Phe Leu Trp Leu Asp Pro Aia Al a Arg Asp Ala Val1 90 Ser His Gly Ala Ile Ser Ala Lys Lys Trp Arg Ser Ser 115 Tyr Lys Lys Asn 100 Al a Lys Asn Ile Leu Leu Giu Ile Giu Leu Leu Met 120 Asp Arg Gin Ala Tyr 125 Thr Ile Arg Ser Leu Glu 130 Phe Arg Giu 135 Al a Val Ala Ala His Ser Gly Asn Lys Leu Leu Leu Val Ser Ser Arg Tyr Giu 145 Pro Gly 160 Ser Giu Val Asp Met 165 Glu Leu Ala Ser Tyr 170 Ala Lys Lys Leu 175 Giu Ser Ile Thr 180 Gin Lys Arg <210> 119 <211> 568 <212> DNA <213> Eucalyptus grandis <400> 119 tcgtcaccca ggttctacgt.
ccaaagtcaa Ctaccgcttt cagtactcgt tagcagggga catcagatcc ctttctctca gcgcagaccc ttgaatataa attcctcacc ccaggacggc ctcgatcacg caacgtacat ggtcagtctc tgcatctgcc ggctacctcc ggtgcacttt.
attcgagtgt cacggtttat aacaacaacc aaagt aattc tcagattact ggtggtcttg tgggatgatc cctgggaccc gtcactttct gggcaatgtg gtcttcagta gtccttcc gagcctctgg caaactctat gcaccgctaa caaagatggg ttggcggcgg tccgtggacc cgaatatccg gaggtcaatt atccgtatta cacactttcc ggtaaacctc aatggacgtt taaatccctt gatgacttqg gtgcaccgct agttggcgat ttacgagggg cagccagtgt tccatcagga t ccggt ct tc ctcgacgatt gcacgaggag ttggatggtc gcgaatgtaa atcaatagca ccagctcttt ctataagata 120 180 240 300 360 420 480 540 568 <210> 120 <211> 360 <212> DNA <213> Eucalyptus grandis <400> 120 WO 00/22092 WO 0022092PCT/NZ99/OO 169 cgacatgttc gcaggtcggt tgctggtgga ggtccattcc ctgtccggat cact t atggc gacaaggccg acccagactg tgt ac caa tg atcaacggta gacacgactt attactactt Sto tottog c ctgaatctca tttctggtgg ctactaactg gcgcagccaa ccggcaatgc tttctcttta tccgcctctt tagtgttgtc ttaoactggt otgogotttg tcttaccctc otogoogtoa aootggcaaa attgaogoga oaagoatgga gatggtgotg aagttcgtca ottaoggtca aatgtaogac attggcgttg acaogacaot attactotgg ctcaatcttc 120 180 240 300 360 <210> 121 <211> 375 <212> DNA <213> Eucalyptus grandis <400> 121 tattatgatg ogaatttaat attootoaat ggagoogttt otgtatcctt ttgcaaaggg gtttggtoag gaotcattgo qctgggtcaa cccaaatcgg atgctttatc tgtttagtga aaaaaaaaaa aaaaa tgctatagca gataggtggg gotoatggga aatoacoata tggototttc cattactcca gttgggttta gtcttottoa agacgtgggo tcacttcttt oagttcooct ttctttttta goaggaoaat gtttttgggt goaooootac gggtggoaat gataggttat aatgagatga ttaoagtgtg ottggotoat oattgtttto cagcoccca tctttttaat tcatgttgac <210> 122 <211> 590 <212> DNA <213> Eucalyptus grandis <400;.
oacgactttg agocatooaa otggatatac otggagatog cotttcggat gagcaggcat ttottaoato actocoggct aagtctogto atggggtggt 122 gaagatggtg gtoatcagtt ggttcggtga gtgtaotgta cgtotacaoo tgcooaatot aattoggttg atctgootto t t tatggoao gttggaatcg gtgttoogcc goggatatga otgaggatat taoctaagag aggt tctocg ggtatggcta tgtatcottg tcaotggooa ttttoatotc atgactggtg agatgctagt agacaagaoa attgactggg gootgoattc gtgggotctt cgggggaggt gaootooato attoatogtg oattgotgog gagaaacgag octgoatogc gaatggggaa ttoaaaatgo aagggttoag gggtoagttg ttgaagtggt ooottgattg ootgagatta aotggtatto cagttttggg taotaaaaga aagaagttgg aotgccaogg caoogatoaa agattttott tggaaogatt tttaotgotc gcaaotatgo t tgagatgca 120 180 240 300 360 420 480 540 590 <210> <211> <212> <213> 123 590
DNA
Eucalyptus grandis <400> 123 oaotgcactq agctgagtto tggtgototo tgocatotca ogggtocgtc tgtataotgo tcggottcat o aa tgcat a tggaatttac tctattoct attcotaaga oaaggcogac aotgttctto tgctggtaog aoagaggatg gtgaooaaga oaagtootgc atggcoagcg ootttcactt gggaagttca agttoggaaa ooottgooga gacggcttot aagataaaac t cgtgaoagg gagat gott t gotgggogao goaggotgaa ooatctttct ttgtgcaatc ctcatacatg ooatooatoo tgatcggtoa tgagtggggg gtatagaatg tcgtgggaco gggct cggtg gttccttcag tattgtotac gctcotgta ttoattgatt gtqaaaaatq aoagttgoag gaaogagtag oaoaatogag gcaoooatca gagatattct aggattgctt tgctttctcc tccttcctaa coataccttt cgacogcotoC aggoaataag ggtggattta gatggacatc atotoaooga tctctogoaa acctcaacgt oggogctoto <210> 124 <211> 619 WO 00/22092 WO 0022092PCT/NZ99/OO1 69 <212> DNA <213> Eucalyptus grandis <400> 124 ggaaagggtg gtgacaagaa aacattccaa tattcaacat agatcactgc ttatgtccca attgcagcaa cattcatgga ttgaaggaag caattcatgt gagattggat ggatatacgg gctcgaggtt. ggatctccat cccataaatc tttcagatcg attttactaa gcaggcattg gagagattgg catatataaa tattgcattc tgcctgcat ttacatcgac ggaggatatt gaaaagctta acagggaggg tatcagctgt ttctgtcaca ttactgtatg tctgaaccaa tcctatatgg taccattgtg aagaagagag gaggagggga gagaagcgct cttccaccat gg c tatgagg gaagatatct cctccacgcc gttcttcgat t atggt ta ca tatcccctca ctggcaaaag tggaaggtta ttggtcaatc ctactaatcc acaagactga taacagggtt cagcattcaa gggcattggg atgggagact cttcaatcct aactgaatcc tgatgatgag gccagtcttc agcaactctt gtggggcaaa taagatgcat gggttccgct gtccattgag gaagtggt tg cttgattgct 120 180 240 300 360 420 480 540 600 619 <210> 125 <211> 429 <212> DNA <213> Eucalyptus grandis <400> 125 cgtcctctta gccccttgcc gactggcgtc ttcctcgcca gcagaccgag actcacccca tagcgtgaac ggtgaagtca caccaagaac tgctacgatg cgcctccaag tgcgccatcg cggtgatgcc ctgactctca tcgtctzcta cagcagttgc cgctcctggg agatgacatg ccatcgacgc gcaacaagtg aaggtgccac agttcgtcac aagtccatca caccgcccat gaagaagtgc caattggcgc gaccgacaag ctattccaag caagcacgaa ttcatcatca gcccaggccg agtagcggtg tggctgcacg tgcagcagcg acatacggtg tatggtacca tgctcctcca taggcaagga gcagctgcac ggacgtcaga cgactgactg cctccactag atatcggctc 120 180 240 300 360 420 429 <210> <211> <212> <213> 126 534
DNA
Eucalyptus grandis <400> 126 ctggaatcat cccaaaagaa ctcccacact ttagatattt.
actccgattt aagctgtcct gtagcctctg aggaacgagt ctqaqtc4qQc cctttatttc aaggttgtgg attccctttt ccaaacaaaa gccgtttgag gagttgaccc tgttccgaca ttgttcttat agaccgtgtca tgtgactgtg cttgcagctg tttcttcctt gtctgtctct agaagttacc tgttgcttct aatgaatacc taatgccgat aatctataqa cactttcaga aatcaatacc cattaatttt ttttttcttt tctgttatgg ttaaccccac ggtggtcggt gagaatgcca gacoacatta gaggaaggag acacacccat atctctcttc tattattatc actgtggatc tcaaccgatc taatcgccgg gaataagatc aattaaccat agagaggaaa atatacaata attttgtaat atttgttccg tccgagaacc tgtcaagatt ttcacacaac agtgcaagag cciat <210> 127 <211> 450 <212> DNA <213> Eucalyptus grandis <400> 127 aaagcactga gtgagagctg gaactgaagt gagatagaga tggagtgacg aggaagcctc tcccgctcca cacctccttc gctgctgccc cgccgccgat ctcctcttct ccgagacccg gactgactga tgttagagag agagagaatt ccctcccttc ttcaccaaac gttcgctctc cctccattgc gtagcaccgt cgccgccgct gaatcgcgaa ccgcttgtcg agcaccgcga WO 00/22092PC/Z9016 PCT/NZ99/00169 tcgcccccga gcgagcgaga gcgagagcga gagcgagagg ggaggacatg gaagcgaatg ccgggatggt ggccggatcc tacaagcgga acgagctggt ccggatacgc cacgactccg acagcgcgcc caagcccctg aagcacttgg atggccagat gtgtcagatt tgtggtgata ccgttggact ttcggccagt ggtgatgtgt <210> 128 <211> 302 <212> DNA <213> Eucalyptus grandis 300 360 420 450 <400> 128 tcttgtccag gtttactcaa ggttttaggt aaagcagcgg atgatggagc attctcagaa atcccgccca tgactctcct gatcatgaac atgccatcaa taatgggtat gattcgtggg tctgggtcgc gtccatctct acccattcct cc ggagtcaaca ctctatatct attgtatgga gtcccctctt aagggattgc caaacttcac ttaaatggac ggctgttggc tggtaggcta tcgggaagca cgtcacctca atcgctgttg gggatctccg tttttcgcct agaccggatg 120 180 240 300 <210> 129 <211> 177 <212> PRT <213> Eucalyptus grandis <400> 129 Val Thr Gin Phe 1 Ser Ile Arg Arg Met Val Asn Leu Tyr Cys Thr Ala Leu Thr Asn Asn Asn Arg 10 Gly Ala Ser Gly Thr Leu Ser Phe Tyr Val Gin Asp 25 Lys Lys Val Ile Ser Gly Leu Pro 40 Val Val Asn Ser Ile Thr Pro Asn Ser Thr Ser Asp Ala Phe Asn Lys Met His Asp 55 Lys Leu Asp Asp Ser Leu Val1 Val1 Gly Gly Leu Met Gly Lys Ala Arg Gly Ala Leu Val Val Ser Al a Trp Asp Asp Leu 90 Al a Gly Gly Met Thr Trp Leu Asp Gly Pro Cys Thr 115 Phe Ser Asn Gly Asp Ala Ser 105 Ser Pro Gly Thr Ala Asn Val Thr 120 Asp Asp Pro Ala Thr 125 Phe Leu Arg Gly 110 Ser Val Thr Ser Gin Val Ile Arg Val 130 His Phe Gly 135 Gly Ile Asn Ser Thr 140 Gly Gly Gin Cys Gin Phe Tyr 145 Al a Leu Glu 155 Pro Pro Ala Leu Asp Pro Phe Glu 165 Val Phe Ser Tyr Tyr Ser Gin 175 <210> <211> <212> <213> 130 118
PRT
Eucalyptus grandis <400> Met Phe Asp 1 130 Lys Ala Ala Leu Phe Ala Phe Ser Leu Leu Ala Val Thr WO 00/22092 WO 0022092PCT/NZ99/0I 69 Tyr Gly Gin Gin Val Gly Thr Gin Thr Ala Glu Ser His Pro Pro Leu 25 Thr Trp, Gin Lys Cys Thr Thr Ala Gly Gly Cys Thr Asn Val Ser Gly 40 Glv Ser Val Val Ile Asp Ala Asn Trp Arg Trp Val His Ser Ile Asn 55 Gly Thr Thr Asn Cys Tyr Thr Gly Gin Ala Trp Asn Thr Thr Leu Cys 70 75 Pro Asp Asp Thr Thr Cys Ala Ala Asn Cys Ala Leu Asp Gly Ala Asp 90 Tyr Ser Gly Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ala Leu Thr Leu 100 105 110 Lys Phe. Val Thr Gin Ser 115 <210> 131 <211> 93 <212> PRT <213> Eucalyptus grandis <400> 131 Tyr Tyr Asp Ala Asn Leu Ile Ala Ile Ala Val Gly Phe Ser Arg Thr 1 5 10 Ile Tyr Ser Val Ile Pro Gin Trp Ser Arg Leu Ile Gly Gly Val Phe 25 Phe Ser Phe Trp Val Leu Ala His Leu Tyr Pro Phe Ala Lys Gly Leu 40 Met Gly Arg Arg Gly Arg Thr Pro Thr Ile Val Phe Val Trp Ser Gly 55 Leu Ile Ala Ile Thr Ilie Ser Leu Leu Trp, Val Ala lie Ser Pro Pro 70 75 Ala Gly Ser Thr Gin Ile Giy Gly Ser Phe Gin Phe Pro <210> 132 <211> 196 <212> PRT <213> Eucalyptus grandis <400> 132 Thr Thr Leu Glu Asp Gly Gly Vai Pro Pro Asp Ala Ser Pro Ala Ser 1 5 10 Leu Leu Lys Giu Ala Ile Gin Val Ile Ser Cys Gly Tyr Glu Asp Lys 25 Thr Glu Trp Gly Lys Glu Val Gly Trp Ile Tyr Gly Ser Val Thr Glu 40 Asp Ile Leu Thr Gly Phe Lys Met His Cys His Gly Trp Arg Ser Val 55 Tyr Cys Ile Pro Lys Arg Pro Ala Phe Lys Gly Ser Ala Pro Ile Asn 70 75 Leu Ser Asp Arg Leu His Gin Val Leu Arg Trp Ala Leu Gly Ser Val 90 Glu Ile Phe Leu Ser Arg His Cys Pro Ile Trp Tyr Gly Tyr Gly Gly 100 105 110 Gly Leu Lys Trp Leu Glu Arg Phe Ser Tyr Ile Asn Ser Val Val Tyr 115 120 125 Pro Trp Thr Ser Ilie Pro Leu Ile Val Tyr Cys Ser Leu Pro Ala Ile WO 00/22092 WO 0022092PCT/NZ99/00I 69 Cys 145 Ser Leu Glu Thr 1 Se r Ser Leu Trp Gly Gly Thr Ala Ala 145 Gly Pro Val1 Gly 1 Arg Gly Ser 130 Leu Leu Thr Leu Val Phe Glu Met Gin 180 Gin Phe Trp 195 <210> 133 <211> 196 <212> PRT <213> Euc~ <400> 133 Ala Leu Ile Ile Pro Leu Val Lys Asn Leu Asp Ala Tyr Giu Asp Ser Val Thr Trp, Thr Ser 100 Ala Pro Ile 115 Thr Gly Ser 130 Ser Gly Ary Ile Tyr Pro Ala Leu Ser 180 Ser Phe Leu 195 <210> 134 <211> 206 <212> PRT <213> Euca <400> 134 Lys Gly Gly Thr Glu Ser met Glu Gly Leu Glu Lys 135 Gly Gin Phe Ile 1S0 Met Ala Leu Phe 165 Trp Gly Gly Vai alyptus grandis 140 Val Pro Glu Ile Ser Asn Tyr Ala 155 160 Ile Ser Ile Ala Ala Thr Gly Ile 170 175 Gly Ile Asp Asp Trp Trp Arg Asn 185 190 Pro 5 Ala Gly Ser Lys Giu Val Asn Val1 Leu Phe 165 Leu Lys Phe Pro Val1 55 Glu Val Cys Thr Ile 135 Phe Ser Phe Gly Asn Gin Gly Arg 25 Pro Gly Ala 40 Ala Glu Ala Trp Gly Glu Val Thr Gly 90 Vai Thr Lys 105 Asp Arg Leu 120 Phe Phe Ser Leu Gin Arg Ile Phe Leu 170 Ser Tyr Met Phe Ile Asp Pro Leu Ile Arg 75 Tyr Arg His Arg Ile 155 Ile Leu Thr Ser Val Arg Asp Gin Asn 140 Al a Val Ala Val1 Ala Gly Met Ala Val 125 Asn Tyr Tyr Asp Leu Ile Trp His Phe 110 Leu Al a Leu Cy s His Arg Ser Ile As n Arg Arg Ile As n Phe Pro Arg Cys Tyr Arg Gly Trp Met Val 160 Leu Ser Gly Lys 185 Phe Ile Val Gin 175 Ser Leu Ser 190 iyptus grandis Asp Lys Asn Ty 5 Asn Ile Pro Ii Tyr Asp Asp Gi1 40 Arg Phe Gly Gi1 55 r Ile Asp 10 Phe Asn 25 Arg Ser Ser Pro Lys Lys Arg Ala Gly Lys Met Glu Asp Ile Glu Giu Leu Leu Met Ser Gin Lys Val Phe Ilie Ala Ala Thr WO 00/22092 WO 0022092PCT/NZ99/00 169 Ph e Leu Giu Ile Cy s Ser 145 Ile Leu Leu Met
I
His Thr Glu Thr Al a Lys Val1 Met 1 Phe Glu Thr Met Glu Lys Glu Trp Gly Leu Thr 115 Met Pro 130 Asp Arg Leu Leu Lys Trp Thr Ser 195 <210> <211> <212> <213> <400> Leu Leu Ala Gin Trp Lys Val Thr Lys Asn Thr Asp Thr Tyr Thr Lys 115 <210> <211> <212> <213> <400> Asn Thr Val Leu Leu Ser Val Asp Gin Al a Lys 100 Gly Pro Leu Ser Leu 180 Ile Gly Ile Glu Phe Arg Asn Arg 165 Giu Leu Gly 70 His Ile Lys Pro Gin 150 His Arg Leu Leu ValI Giy Met Al a 135 Val1 Cys Leu Ile Pro Ile Trp His 120 Phe Leu Pro Al a Al a 200 135 126
PRT
Eucalyptus grandis 135 Gin Thr Gly Val Phe 5 Ala Val Gly Lys Giu Lys Cys Ser Ser Giy 40 Ilie Asp Ala Asn Trp 55 Cys Tyr Asp Gly Asn 70 Cys Ala Ser Lys Cys Gly Ala Ser Thr Ser 100 His Giu Tyr Gly Thr 120 136 51
PRT
Eucalyptus grandis 136 Gly Gly Arg Leu Ile 5 lie Asn Ala Asp Glu Giy Gin Thr Cys Gin 40 Pro Ser Ile 105 Ala Lys Arg Ile Tyr 185 Tyr Leu Gin 25 Gly Arg Lys Ala Gly 105 Asn Se r Cys 90 Tyr Arg Gly Trp Trp 170 Ile Cys Al a 10 Thr Ser Trp Trp Ile 90 Asp Ile Thr 75y Gly Gly Ser Al a 155 Tyr As n Ile Thr Giu Cys Leu Thr 75 Giu Ala Gly Asn Tyr Ser Trp Al a 140 Leu Gly Thr Leu Leu Thr Thr His Asp Gly Leu Ser Pro Giu Val1 Ile 125 Pro Gly Tyr Ile Pro 205 Leu His Se r Gly Lys Al a Thr Arg 125 Al a Asp Thr 110 Ser Ile Ser As n Val1 190 Al a Gly Pro Val1 Thr Cys Thr Leu 110 Leu Thr Lys Giu Ile As n Ile Gly 175 Tyr Thr Lys Asn Ser Ser Tyr Lys Leu Thr Asp Tyr Leu Glu 160 Arg Pro Al a Met Gly Asp Ser Ser Phe Ala Gly Ser His Asn Arg Asn Giu 10 Asn Ala Arg Ile Arg Ser Val Gin 25 Ile Cys Arg Asp Glu Ile Giu Leu WO 00/22092 WO 0022092PCT/NZ99/00I 69 <210> 137 <211> 54 <212> PRT <213> Eucalyptus grandis <400> 137 Met Glu Ala Asn Ala Gly Met Val Ala Gly Leu Val Arg His Leu Asp Ser Ala Ser Ile Arg His Asp Ser Asp 25 Gly Gin Met Cys Gin Ile 40 Gly Asp Val Ser Cys Ser Tyr Lys Arg Asn Glu Ala Pro Lys Pro Leu Lys Gly Asp Thr Val Gly Leu <210> <211> <212> <213> 138 100
PRT
Eucalyptus grandis <400> 138 Cys Pro Gly Val Thr Ser Phe Lys Trp Leu Lys Leu Lys Val Leu Gly Val Asn Thr Asn Phe Ala Ala Asp Asp 25 Ile Ala Phe Ser Ile Thr Ser Leu Pro Pro Met Met Asn Ile Thr Met Giu Leu Tyr Leu Leu Ile Pro Ser Ile Val Trp Arg Met Gly Leu 55 Val1 Ala Gly Ser Pro Gly Met Ile Arg Ser Gly 70 Ser Pro Ser Leu Tyr Phe Ser Pro Lys Gly Ser Arg Pro Leu Pro Ile Gly Leu Leu Gly Gin Asp Arg Met 100 <210> 139 <211> <212> <213> 2906
DNA
Eucalyptus grandis <4 00 >13 9 gggaggccga Ecaccatcct tttcttttgg cctgccttcg tcgcatgctg CCquaacgat gcgccaccag tgatggggcc ggttgctctt tgcgcttgtt tgatggaagc ttttccgcgc gcctcttgct ccgttcgttc cgtcccgttt ccgcgccatt actcgaagcc attgtggcct atttttgctg tttggtgaag gctgttcgtc cttgagcaat t tgaatggta ccgactcttt ttttttcgtg gttcccttcc ttggggctcc tgaagttctt acagccttcg Ccctttcaag agtttgaggc tcctcaaatc caaggccggg tggaggttgc actttgtgct ccaagtctat tataaaaggg ctttccattg cttgaagatc tttctgagag cgagcgtttg ggttgaagcc catct-ctgag cactcaggaa cgtgtgggag tgagtatctg tgagcttgac tggcaatggc cctcccccat ttgcgtttaa tcctcttcat aagaatttag gacgagaccc aagggcaaag gagagcagag gcgattgtgt cacatccgtg cacttcaaag tttgagccat gtcgagtttc tcctcatttt gccctccaat ttcgggattt a cat gg ctga tctctgctca gcatcttgca caaagcttct cgcctccatg tgaacgtcca aagagcttgc tcactgcctc tcaatcgcca 120 180 240 300 360 420 480 540 600 660 720 WO 00/22092 WO 0022092PCT/NZ99/001 69 tctctccgct agtccactgc cctccaacat gtactcccag ggctgagcgc qtgcactctc tccccacgga tgtttacatc acaaggactg tggaaccacc tcgcgtcccc gtggccctat gggcaagcct agcacataaa cccagagtca cactgctgat aattgctgga tggactctac accaggtgct cttccaccct tgtgttgaaa gaacttgaca cttggtcgtg gatgaagaaa ttcctcccag gggagtcttc gacttgtgga tggcaaatcg agacttcttc catgcagaga gactgccgtg gtaccttgaa tgtcgagtaa tttcaaggaa gtttcctttt ggggtttgta tccgtatttc <210 <211- <212:' aagctcttcc tacaagggga gtcctgagga ttcgagcaca gtcctcgaga gagaagttct tactttgctc ctggatcaag gatattactc tgtggccagc ttcagaaatg t tggaaagat gatctgatca ttaggtgtta gacatatact ctcatcgcca agcaaggata cgagttgtcc gacatgagca gagatcgagg gataagaaga gggcttgttg gttggaggtg atgtacgacc atgaaccggg gttcaaccgg ttgccaacct ggctaccaca aacaagtgca attgaagaga tatggcttct atgttctatg acaaagagac taaacattgg tttggtcctt cccatcaatt gtactaaaaa atgacaagga agaacatgat aggcggagga agttccagga tgatccagct tggatagggt aggacgacgt ttcgtgccct ctcggattct gccttgagaa agaagggagt acactgagga tcggaaacta cacagtgtac ggaagaaatt tgaaccacac cagtggggca acgggatcga tctactttgc aactcctctt agcctattat agtggtatgg acaggaggaa tcatcgaaaa tgaggaatgg ctatctatga ttgccacttg ttgatcctta agattgacca agtatacatg ggaagcatgt ccctcaagta agattgttac aaattgtttg tttctcttct cagtaaatgg aaaaaa aagcttgcac ggtgaatgcc gtatctgacc gatcgggctc cctgttggat tcccatggtc ccttggttat agaggaagaa cattatcact agtttttggg cgtccgcaag tgtcgcgagc cagtgatgga aatagcccat tgaggaaaag cgacttcatt gtatgagagt cgtcttcgac ttacaccgaa cagcgatgtt tttcaccatg caagaactcc ggattcgaag gtacaagctg agagctctac agctttcggg caatggtgga ccatggtgac gtcccactgg gaaaatatat gactaacctt tcgcccactg cagaagacgg aatttgggat ttgtttccat ttcattttct Cctctgcttg agaatccaga tcgctcaaac gagcgggggt ct cc ttgagg ttcaacgtcg ccggataccq atgcttcacc cggcttcttc accgagtact tgga tt tccc gaacttgctg aacattgttg gccctcgaga taccacttct atcaccagca cacatgaact ccgaagttca caggagcggc gagaacaagg gcaaggctgg aagttgaggg gacttggaag aatggccagt cgctacat ct ttgaccgtgg ccagctgaga caggcggccg gacgagatct tctgagaggc gatcggcgcg gcacagtctg aagcattgga tgccaagagc tccgcgaatg tttcaaattg aattcctcca atgtgttctc ccgagacccc ggggtgacac ctcccgaccc tgatcatgtc gtggccaggt gcattaagca cagacgcggt cccacattct ggttcgaggt gagagttgca cttccttgtt agacgaagta cttgccagtt ccttccaaga tcactcttcc acattgtttc ggttgaaatc aacacttgtg accgtgtcaa aactcgccaa agcagtctga tcaggtggat gtgacacgaa ttgaggccat tcattgtgca agcttcttgt caaagggtgc tgttgaacct agagtcgccg ttcctccggc cttttgaagt gatctttttc tttgcatttt tccgtgccgc 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2160 2220 2280 2340 2400 2460 2520 2580 2640 2700 2760 2820 2880 2906 140 1592
DN~A
<213> Pinus radiata <400> 140 ggccaaggaa agttcaggag gcttcaaaga gtggtggcca atggctgcca ttctctgaac aaagaaaaa t ggggtttgga tgttacacgt accaaacgtt gcttcggaaa aaaatttgtg ggattcgtgc acttgactac gtatccaagg ggaagggaga aatttggaag Cctgcttttg tctgcatatg gtgagagcca ggcttgtata tgttctggtg gatcacaccc agtgttggtt aaagaatata aattatactc tccatcaatt aacacgatca ttcttctcca aaaagatccc attctgctgc gttcagaata tggctgacat atcgctatga gacttggaaa aagattttgt ttcaagattt ttgaggcarc ctcctagcta tgtttacagt atgggcagga gggttttaac gcacattgct accccaaggt t cagctgaaa agttatcaat tggcttgcca ctggagcaca acgcaaagac tcgatttgac gacccccatg tcatcttgat tttcgaaaaa tttgttqaag tgggagtcac aaagcaggat tacttgcccc agcttactta catcgcatgg ctgccttggg ggggataat t atcaaagatt tttgccaagg tttgctgttg aagaaccaag tgggctatcc gtqaaaqtqt ataaatacaa ttacatctgt gaaacattta tgacaatgcg gaagctctca atgaacgtgc ttcatggagt ggttaaggtg gttatgcagc gggaatattg atagccatag WO 00/22092PC/Z/0I6 PCT/NZ99/00169 acaaagaata aacaaagggc gggaaagcct ccatgacact ctatgcgtac ttggggaagc tttaaacagc tattggaagg gtggcagcta catgtatttc tactagtatc gggtacgata tatgttatga tcattatgtg atcaattgga attcttcagg cctggagtgc ggatcaacac atcttgacac tcaatacaaa cgctcttcaa aaagtttgca gctacttgtg tagtggctag gtgtagtgaa tacagattat gcacatctac tgtacataaa t tgatggcac aggcagtgaa ttggctggtg agggtcattg accctggaat acgagattat tagagatttt tgaagattgg gtcatagata atagtagtgt ttaatgtatc ataaaatttt gttgaaattt aaaaaaaaaa tagtggcatc aggacaatgt gcctccaagg gccttttcca accagcagtc aaagctgatg agaggccagt agatggctca tgctcxtttgg cttgtagtga tctattttca ctacacggct ttatcctggt aa tctgcggcat tggcgtttac ctggtacttt tgtgatcata ttctatgacc agaattcgga tcaactgttt tggtgtccga cacaagtaac atttatttct accgtggaaa atagacctac cttgaataaa ttgattttac gtgatcacca taaatgaaaa ttatggaggg atttttttga gaacacaaga atgctgcaat atggcaggga attgtttttg agtggctaga tgagatgcat tctagaggtc gagcatggaa 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1592 <210> <211> <212> <213> 141 3747
DNA
Eucalyptus grandis <400> 141 cggggatttc caacgggact cgtgtacggt ccgcaattac cggctcggta gaccttgatt gcgcgcctgc cgctttctca actcattggg act tgcggag tgcaacttcg tgcttgcgct ttgggaagtc gctagacaca cctatctgga ccgacgaagg attgctgatg ccataccgaa gtgacacatc tggttcgcat actcatgttg gctgtcgact aatacagtgc t ctgatgatg agaaagtggg ttttctcaga agagcaatga gcgcagaaag aactctcgtg atcgagggaa caacatcata aatgctccgt cgtgaagcaa ttccctcaga ttctttgatg ggatgtgttt caagtctcca ttccaaaatg gggaggtcta tgtgagttag tattgtgtgg acgccaagct aggtcgacac tat aaaga ag tcgcgagaag aggctgttgg ccatttgcaa gtggcactcc gatcgacaat tcagcagtgt agaatagagt ctgagaaaga cttcagagcc ccgtaataat cagtcgacag act ca tgggt acagactatc tcttcgtgag tttccatcct gtgctgcgat tacctttctg agattgacta aaagagacta cgccggagga accaccctgg atgaacttcc aaaaggccgg acatcctcaa tgtgcttcct gatttgatgg ttaacatgaa tcaataggca ccccattgac tcgtaacaac tataagcaga ctcactcatt aattgtgagc cgaaattaac tagtggatcc tgaaagaata attggccaac ggttgatgag ggcttgtgtc atttgaagcq ggcagctcaa ttctacgttg ggagagctgg ggctcaagtt actctcgacc aatgcgattg cgcctatcca gttggatcag tgcaaggtat tacagtcgat tgctgtggac gctctcattc caaaaagtat cttgaaggat tgaggagttc aggttggtct aatgattcag tcggctggtg agcggaaaat ccttgactgc gatggatcct tatcgatcgc agggctggat agcactttac gtcaatggga tccgccccat qctggtttag aggcacccca ggataacaat cctcactaaa aaagaattcg cgaggatact atgatggaat aaaggcgagg gaatatgaga aactcgatgg ctcaatgatc gatagtgaat aaggacaaaa ccaccagagc gttattccca atcattttgg t tgtggctta ttcccgaagt gaaaaagaag ccaatgaaag tatcctgttg gagtctcttg tcgattgagc aaaattcagc aaagttcgag atgcaggatg gttttcctcg tatgtttcca gctctggtga gatcattatg caagttggta agtqfatcgat ggaatccaag ggatacggac gtttgttttg tgacgcaaat tgaaccgtca ggctttacac ttcacacagg gggaacaaaa ggcacgagct ccacttgggt ccggggttcc tcttcgtggC ttaaggaagg ctgatgctga.
ctcaggacac acaatgatga agaataagaa agcagatgga ttgccaaaag cacttttctt catccatcat ggtctcccgt gtgaaccttc aaccaccttt ataaagtctc ttgaaacggc cccgtgcgcc cttcttttgt ttaatgcttt ggacaccttg gaagtagtgg gagagaagag gagtatctgc tcaactatag gaaatttgtg atgccaaccg gaccagttta ccccttctat gcaccaaaat gggcggtagg gatccgctag tttatacttc aaacagctat gctggagctc cttctcgtct atcgccaaga cctgtgcaac ttgtcaagag aagaaaagcg gagaaatgaa agggattcat gactgggaac gaagaaggcc agagaagcaa caaactcgca ccattaccga atgtgagatt gaataggatt agagcttgct gatcactgcc ttgttatctt cgactttgca tgagttctac gaaggaacga ggtagcaaaa gcctggaaat agcccacgac acccggtttc aattctgaca taatgctgtt ctatgtacag aaataccgtt tgt cgggaca gccgaattta 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2160 WO 00/22092 WO 0022092PCTINZ99/00I 69 ccaaagccct aagcccacta gccatcttca tctcagatga ttggcaaatg catgtcatta tatggctcgg tcggtctatt gacagactgc cattgtccac tacataaaca cctgcgatct gtgctatttc agtggcgtga tcagcccatc aacttcaccg aagtggacga gttgccgggt aaggtcttct ggtaggcaga tctctctcgt agagctgcag ctcaagctgt gttggaaatt gacatttttc aaagaaagag tgatcaaaat cctcatcatg aagatctctc accttggaga qctttgagaa gaggagttcc gctgtggcta ttactgagga gcatgccctt accaagttct tttggtatgg ccatcgttta gcctccttac t tggtct t tt gcattgagga tctttgccgt tcactaccaa cgctgctgat tctccgatgc ttgcattctg acaggactcc ctgggtgaag ttctatagat ttttgcagtt tgctaaagaa ctcactatcc tattccctgt ctaaaaaaaa ctcctggtgt agaagtttac gattgacaat aacttttggc tgaatctgcc tgaagagaag tatcttaaca aaggcctgca ccgatgggct atttggcgga tccattcaca tggaaagt tc cctctccatc ctggtggcgt cttccaagga agcagccgac acccccgacc gctgaacaaa ggtgattctt gaccattgtg atcgatccat tgttgaattg ttgcgcctct agggtgat ta caaaaattcc atttcttgaa aaaaaaa ggctgctgct cgagactcaa tatgatgagc ttgtccactg catccgtcta accgcatggg gggttcaaga ttcaagggat ctgggttccg ggccgcctca tccctccctc atcattccaa atcgtcacaa aacgagcagt ttcctgaaga gacgcggagt actcttctca ggttatgagg catctctatc gttctctggt tcgtgagcaa cgcgagtgt t tgaagattgg ttgtattcat tggctttgtg caatttaaca cttgttgctg aaagggaaga atgagagatc tgtttattga tgctgatcaa gcaaagagat tgcactgcag cagcgcccat tggaaatatt agtggcttca ttgtcgctta cgctttccaa gtgtgctcga tctgggtgat tgttagctgg ttggtgagct tcgtgaacat cgtggggacc cattcctgaa cggtgttctg atccgatgct ggcttgtgtt ggaaataccg gaattgatat tacttgtaaa ttagaatgtg cccttcaaag cctaaatgcc aatgctgatc gtcgacgctg agaagccatt cggttggata aggatggagg taatttgtca cttgagtaga aagattggcg ttgcacaatt cttggcaagc gctgcgatgg tggaggtgtc cctagacacc ctacatgatc ggtgggtgtc cctctttggc aggtctcatg gcttctgtct gacctctccc tatcaaggat agtttatgat gtagaggcga atggtacgag cgatgcatgg 2220 2280 2340 2400 2460 2520 2580 2640 2700 2760 2820 2880 2940 3000 3060 3120 3180 3240 3300 3360 3420 3480 3540 3600 3660 3720 3747 <210> 142 <211> 770 <212> DNA <213> Eucalyptus grandis <400> 142 ggttaaagga tgcaataatt ccatagtggg tgaacgctcg tgggtgcaga ctattaccaa tgcctattgg gatagggaag agattgggaa agatgtggcc gggatgggtt ttacatacgc atggtacagt tatataagcg aaaaatactc cgagaagaga gcagttctcg gagagttgct gtcctgtcca tagtattata cttcttagtt tgtaaataag aactttcgat actatcctgg accggcagtt ttctgtcaac tcccacggtt cgcctcgatt acagaatctg aacacaaaga attgtaaaca ttgggaataa gatggatttc cc tagg cat g gagcaccttt ggacaagcc t gtaatctgcg tqatoatatc atctcttttg gctttttgag ctggagttga aaatagcttc taaggaactg aagatggcgt ccgtgatact gagtcaatca gagatcgaac ctgctgtatg actagagtct agatttgaat ttgtoaaact atttaaaaaa agagtgccgc gctgaaggac tctgttagcg cataattgac tgaagaagca ggagaaggca acagttgttt aagttataca tacaaaaata gagttcccac aaactttgcc aataczactat aaaaaaaaaa gttgaacgct acggtgatga gaggggaagg aagaacgcga gatcggccag accatagaag ccaagatgaa tagttttcaa cttatcccta aatataactg ggttgtatcg tatttaaota 120 180 240 300 360 420 480 540 600 660 720 770 <210> 143 <211> 543 <212> DNA <213> Pinus radiata <400> 143 ggacaccatc tctaatggag gtctacagcg tatctatgaa aggtatacat ggaagattta tgctgagaaa ttaatgacgt tggctggagt ttatggcttt tggaagtatg tctcaaaact ggaaagacga gagtcattcc gctatcttga gatgttttac atcttgaagt acagaaatct 120 180 WO 00/22092 WO 0022092PCT/NZ99/001 69 ggttaaaact gtgccatttt ctgttgaaga aagcaccgat ggtattgaag agaagagtgc ataattgcct accggagatg tgccatccaa gggagcttct gccttggttt ataagaattg agtgccttag ctactcccct ggtaaataaa cactgagtgg cacattgata ctatgtaatg accatattct cgtaacgcca tctcgttttc tcccatattt cttggtcctg gggttgctgt ttatatttgt attaggcgat atgaacatat tccgttggaa tacaaagcct ggaacatatt ttgtatatta aaatattagt aattaaagcc aatgtgctcc attgtaaaaa aaaaaaaaaa aaa 240 300 360 420 480 540 543 <210> <211> <212> <213> 144 805
PRT
Eucalyptus grandis <400> 144 Ala Asp Arg Met 1 Asp Arg Al a Gly Pro His Al a Gly Pro 145 As n Pro Met Arg Ser 225 Gly Leu Val1 Glu Val1 Glu Al a Pro Ile Glu Asn 130 Arg Arg Leu Val1 Lys 210 Gln Asp Leu Pro Thr Glu Phe Phe Trp Arg Tyr 115 Phe Pro His Leu Asn 195 Ala Phe Thr Giu Met Leu Ala Glu Gly Val Val1 100 Leu Val1 Thr Leu Giu 180 Al a Glu Glu Al a Al a 260 Val Met 5 Ser Lys Ala Glu Al a Asn His Leu Leu Ser 165 Phe Arg Giu His Glu 245 Pro Phe Leu Thr Arg Ser Ala Gly Ile Val1 70 Leu Val1 Phe Glu Ser 150 Al a Leu Ile Tyr Lys 230 Arg Asp Asn His Lys Ser 55 Leu Ala His Lys Leu 135 Lys Lys Gin Gin Leu 215 Phe Val1 Pro Val1 Arg Giy 40 Glu Lys Val1 Al a Glu 120 Asp Ser Leu Val1 Asn 200 Thr Gln Leu Cys Val1 His 10 Asp Leu Ser Thr Pro 90 Val Leu Glu Giy His 170 Cy s Phe Leu Ile Met 250 Leu Met Ser Leu Arg Glu Ile Gin Arg Gin Arg Leu Ala Pro Asn Asp Tyr Ser Lys Gly 235 Ile Glu Ser Val Arg Aila Glu Pro Glu Asp Phe 140 Gly Lys Lys Leu Pro 220 Leu Gin Lys Pro Ala His Lys Ala Giy Gln Gly 125 Thr Val1 Glu Gly Gin 205 Giu Glu Leu Phe His Phe Gin Leu Ile Val1 Leu 110 Ser Ala Glu Ser Lys5 190 His Thr Arg Leu Leu 270 Gly Arg Leu Leu Ser Ile Phe Leu Asp Val Ser Trp Glu Giu Val Leu Asn Ser Phe Phe Leu 160 Leu His 175 Asn Met Val Leu Pro Tyr Giy Trp 240 Leu Asp 255 Asp Arg Tyr Phe WO 00/22092 WO 0022092PCT/NZ99/0O1 69 Ala Gin Asp Asp Val Leu Gly Tyr Pro Asp Thr Gly Gly Gin Val Val Tvr 305 Ile Arg Lys Asn Pro 385 Glu Asn Thr Tyr Ala 465 Phe His Asp Ser His 545 His Ala Gly Gly Lys 625 Arg Arg Glu Th r Lys 705 Leu 290 Ile Lys Leu Val1 Glu 370 Tyr Leu Ile Ile Trp 450 Asp Gin Met Val1 Ile 530 Pro Leu Arg Lys Asp 610 Lys Trp Tyr Ala Phe 690 Ser Leu Leu Gin Leu Phe 355 Lys Leu Gin Val1 Al a 435 Lys Leu Giu Asn Phe 515 Tyr Glu Cys Leu Asn 595 Arg Met Ile Ile Phe 675 Al a Gly Val1 Asp Gin Pro 340 Gly Gly Glu Giy Ala 420 His Lys Ile Ile Phe 500 Asp Phe Ile Val1 Asp 580 Ser Arg Tyr Ser Cys 660 Gly Th r Tyr Asp Gin Gly 325 Asp Thr Val1 Arg Lys 405 Ser Al a Phe Ala Al a 485 Thr Pro Ala Glu Leu 565 Arg Lys Lys Asp Ser 645 Asp Leu Cys His Phe 725 Vai 310 Leu Al a Giu Val1 Tyr 390 Pro Leu Leu Glu Met 470 Gly Leu Lys Tyr Glu 550 Lys Val Leu Asp Leu 630 Gin Th r Thr Asn Ile 710 Phe 295 Arg Asp Val1 Tyr Arg 375 Thr Asp Leu Giu Glu 455 Asn Ser Pro Phe Thr 535 Leu Asp Lys Arg Ser 615 Ile Met Ly s Val Gly 695 Asp As n Ala Ile Gly Ser 360 Lys Glu Leu Aila Lys 440 Lys His Lys Giy As n 520 Giu Leu Lys Asn Glu 600 Lys Glu As n Gly Val1 680 Gly Pro Lys Leu Thr Thr 345 His Trp Asp Ile His 425 Thr Tyr Thr Asp Leu 505 Ile Gin Phe Lys Leu 585 Leu Asp Lys Arg Val 665 Giu Pro Tvr Cys Glu Pro 330 Thr Ile Ile Val1 Ile 410 Lys Ly s His Asp Thr 490 Tyr Val1 Giu Se r Lys 57 0 Thr Ala Leu Tyr Vali 650 Phe Al a Al a His Ly s 730 Glu 315 Arg Cys Le u Ser Al a 395 Gly Leu Tyr Phe Phe 475 Val Arg Ser Arg Asp 555 Pro Gly As n Giu Lys 635 Arg Val1 Met Glu Gly 715 Ile 300 Giu Ile Gly Arg Arg 380 Ser Asn Gly Pro Ser 460 Ile Gly Val Pro Arg 540 Val Ile Leu Leu Giu 620 Leu Asn Gin Thr Ile 700 Asp Asp Met Leu Gin Val1 365 Phe Giu Tyr Val1 Glu 445 Cys Ile Gin Val1 Giy 525 Leu Giu Ile Val1 Val1 605 Gin Asn Gly Pro Cys 685 Ile Gin Gin Leu Ile Arg 350 Pro Giu Leu Ser Thr 430 Ser Gin Thr Tyr His 510 Ala Lys As n Phe Glu 590 Val1 Ser Gly Giu Al a 670 Gly Val1 Ala Se r His Ile 335 Leu Phe Val1 Ala Asp 415 Gin Asp Phe Ser Giu 495 Gly Asp Ser Lys Thr 575 Trp Val Giu Gin Leu 655 Ile Leu His Al a His 735 Arg 320 Thr Giu Arg Trp Gly 400 Gly Cys Ile Thr Thr 480 Ser Ile Met Phe Glu 560 Met Tyr Gly Met Phe 640 Tyr Tyr Pro Gly Glu 720 Trp WO 00/22092 WO 0022092PCT/NZ99/00 169 Asp Phe Leu 785 Pro Glu Ile 755 Trp Lys 770 Giu. Met Pro Ala Ser 740 His Phe Val1 Lys Gly Ala Met Gin Arg Ile Giu Giu Lys Tyr Thr 745 750 Ser Clu Arg Leu. Leu Asn Leu Thr Ala Val Tyr Gly 760 765 Val Thr Asn Leu Asp Arg Arg Giu Ser Arg Arg Tyr 775 780 Tyr Ala Leu Lys Tyr Arg Pro Leu Ala Gin Ser Val 790 795 800 Glu 805 Met Gly Leu Leu Trp Arg Lys Asp Leu Val1 145 Phe Asp Asp Giu Asp Asp Ile Vai Gly His 305 ':210> 145 ':211> 419 <212> PRT <213> Pini <400> 145 Gly Tyr Pro Phe Val Glu Gin Gly Phe Giu Giu Lys Leu Pro Pro Asn Ile Tyr Ser Leu Leu 100 Ile Val Ile 115 Tyr Asn Arg 130 Thr Arg Cys His Gly Vai Ile Lys Asp 180 Phe Arg Phe 195 Tyr Ile Glu 210 Ser Cys Asn Ser His Arg Ser Ala Ala 260 Lys Gly Gin 275 Val Leu Gly 290 Asp Thr Gly us radiata Vai Gin Giy Giu Asn Trp Ile Pro Ala Phe 70 Ser Leu Met Thr Asn His Tyr Asp Ser Giy 150 Pro Asn 165 Trp Leu.
Asp Phe Ala Ser Tyr Thr 230 Gin Arg 245 Phe Asp Cys Trp Trp Trp Ser Thr 310 Giu Se r Giu His 55 Asp As n Met Arg Gly 135 Gly Val1 Arg Ala Thr 215 Pro Ile Phe Arg Pro 295 Gin Vali Val1 Ser 40 Ile Ser Ser Arg Met 120 Leu Leu Asp Trp Lys 200 Pro Pro Ile Thr Leu 280 Pro Gly Ser Leu 25 His Ala Ala Ala Lys 105 Gly Pro Gly His Leu 185 Gly Met Ser As n Thr 265 Arg His Lys Gin Lys Lys Ala Tyr 90 Lys Ser Leu Asn Thr 170 Arg Tyr Phe Tyr Trp 250 Lys Asp Leu Trp Giu Arg Tyr Al a Pro 75 Gly Asn Ser Pro Trp 155 Gin Lys Al a Al a His 235 le Gly His Val1 Pro 315 His Glv Lys Giy Gin Ser Val1 Gin Trp 140 Ser Asp Ser Ala Val1 220 Leu Asp Ile Gin Leu 300 Phe Asp Arg Trp Phe Gly Giu Arg Gly 125 Asp Thr Phe Val1 Lys 205 Gly Asp Gly Leu Gly 285 Leu Pro Gin Giu Trp Thr Tyr Tyr Al a 110 Phe Glu Gly Val Gly 190 Phe Giu Lys Thr Gin 270 Lys Asn Cys Trp Ile Pro Se r Leu Gin Met Gly Arg Asp Arg 175 Phe Val1 Tyr Asn Ser 255 Giu Pro Giu Asp Al a Leu As n Val1 Pro Leu.
Al a Gly Ala As n 160 Lys Gin Lys Trp Gin 240 Gly Al a Pro As n His 320 WO 00/22092 WO 0022092PCT/NZ99/001 69 Ile Val Ile Ser Ile 385 Asn Trp Met 1 Gly Phe Lys Asp Leu Val1 Trp Lys Gin 145 Val1 Ile His Glu Ser 225 Giu Ser Met Phe Ile Ser 370 Gly Gly His Giu Tyr Lys 355 Ile Arg Arg Lys Al a Phe Arg Leu Cys 390 Gin Tyr Phe Ile Giu 375 Met Leu Ile Asp Arg 360 Ala Lys Al a Leu Trp 345 Arg Ser Ile Thr Thr 330 Giy Thr Ser Gly Cys 410 His Ser Gin Thr Asp 395 Giy Pro Ser Asp Val1 380 Gly His Gly Ile Leu 365 Tyr Ser Arg Ile Gin 350 Asn Al a Trp Tyr Al a Giu Arg Ile Pro 400 Val1 <210> 146 <211> 955 <212> PRT <213> Euca <400> 146 Met Glu Ser Vai Asp Giu Ala Ile Cys Ala Cys Leu Ala Giu Arg Asn Asp Pro Ser Thr Leu 100 Lys Asn Arg 115 Ala Pro Thr 130 Met Giu Giu Ile Pro Ile Met Arg Leu 180 Pro Val Asp 195 Ile Trp Phe 210 Pro Val Asn Lys Glu Gly Thr Val Asp 260 lyptus grandis Gly 5 Lys Lys Arg Asn Gin Asp Val1 Lys Lys Al a 165 Ile Ser Al a Arg Giu 245 Pro Val1 Gly Ala Cys Glu 70 Asp Ser Giu Al a Gin 150 Lys Ile Al a Tyr Ile 230 Pro Met Pro Glu Cys Gly 55 Leu Thr Giu Ser Giu 135 Ile Ser Leu Tyr Ser 215 Thr Ser Lys Leu Cys Val Phe 25 Vai Giu 40 Thr Pro Gly Ser Gly Ile Tyr Asn 105 Trp Lys 120 Lys Glu Ala Asp Lys Leu Ala Leu 185 Pro Leu 200 Trp Vai His Val Glu Leu Giu Pro 265 Asn 10 Val Tyr Phe Arg His 90 Asp Asp Al a Al a Ala 170 Phe Trp Leu Asp Ala 250 Pro Thr Ala Giu Giu Ser 75 Aila Glu Lys Gin Se r 155 Pro Phe Leu Asp Arg 235 Al a Leu Val1 Cys Giy Cys Gin Ile Lys Ala Asn Thr Met Arg His Thr Gly Lys Asn 125 Val Pro 140 Giu Pro Tyr Arg His Tyr Thr Ser 205 Gin Phe 220 Leu Ser Val Asp Ile Thr Asp Lys 285 Giu Giu Giu Ser Al a Ile Asn 110 Lys Pro Leu Thr Arg 190 Ile Pro Al a Phe Ala 270 Val1 Ala Val Cys Asn Gly Arg Met Ala Ala Gin Ser Ser Pro Ile Lys Lys Giu Gin Ser Thr 160 Val Ile 175 Val Thr Ile C%'s Lys Trp Arg Tyr 240 Phe Val 255 Asn Thr Ser Cys Val Leu Ser Ilie Leu Ala Val Asp Tyr Pro 275 280 WO 00/22092 PCT/NZ99/0O169 Tyr Glu 305 Ser Tyr Met Ala Thr 385 Va1 Pro His Leu Asn 465 Gin Gly Asp Gly Pro 545 Gly Ser Phe Leu Phe 625 His Tyr Ser Trp Ala 705 Leu Gly Leu 290 Thr Ile Leu Lys Lys 370 Pro Phe Arg Lys Thr 450 Tyr Val Ile Val Thr 530 Ser Cys Glu Asn Ile 610 Ile Pro Glu Va1 Arg 690 Pro Gly Phe Ser Ala Glu Lys Arg 355 Ala Trp Leu Leu Lys 435 Asn Ser Gly Asp Asn 515 Gly Met Cys Va1 Leu 595 Ser Glu Ser Glu Thr 675 Ser Ile Ser Gly Asp Asp Pro Asp 340 Asp Gin Pro Gly Val 420 Ala Ala Asn Arg Arg 500 Met Cys Pro Ser Tyr 580 Gly Gin Ser Met Lys 660 Glu Vali Asn Val Gly Asp Phe Arg 325 Lys Tyr Lys Gly Ser 405 Tyr Gly Pro Ala Asn 485 Ser Lys Val Asn Cys 565 Arg Glu Met Thr Leu 645 Thr Asp Tyr Leu Glu 725 Gly Gly Ala 310 Ala Ile Glu Ala Asn 390 Ser Val Ala Tyr Val 470 Leu Asp Gly Phe Leu 550 Cys Asp Ile Ser Leu 630 Ile Ala Ile CY S Ser 710 Ile Arg Ala 295 Arg Pro Gin Glu Pro 375 Asn Gly Ser Glu Ile 455 Arg Cys Arg Leu Asn 535 Pro Cys Ser Asp Phe 615 Leu Lys Trp Leu MeL 695 Asp Phe Leu Ala Lys Glu Pro Phe 360 Glu Ser Ala Arg Asn 440 Leu Glu Tyr Tyr Asp 520 Arg Lys Pro Lys Asn 600 Glu Ala Glu Gly Thr 680 Pro Arg Leu Lys Met Trp Phe Ser 345 Lys Glu Arg His Glu 425 Ala Asn Ala Val Ala 505 Gly Gin Pro Ser Arg 585 Tyr Lys Asn Ala Ls 665 Gly Leu Leu Ser Trp Leu Val Tyr 330 Phe Va1 Gly Asp Asp 410 Lys Leu Leu Met Gin 490 Asn Ile Ala Ser Lvs 570 Glu Asp Thr Gly Ile 650 Glu Phe Arg His Arg 730 Leu Ser Pro 315 Phe Val Arg Trp His 395 Ile Arg Va1 Asp Cys 475 Phe Arg Gin Leu Ser 555 Lys Asp Glu Phe Gly 635 His le Lys Pro Gin 715 His Gin Phe 300 Phe Ser Lys Val Ser 380 Pro Glu Pro Arg Cys 460 Phe Pro Asn Gly Tyr 540 Ser Pro Leu His Gly 620 Val Va1 Gly Met Ala 700 Val Cys Arg Glu Cys Gin Glu Asn 365 Met Gly Gly Gly Val 445 Asp Leu Gin Thr Pro 525 Gly Cys Thr Asn Glu 605 Leu Pro Ile Trp His 685 Phe Leu Pro Leu Ser Lys Lys Arg 350 Ala Gin Met Asn Phe 430 Ser His Met Arg Val 510 Val Tyr Ser Lys Ala 590 Arg Ser Glu Ser Ile 670 Cys Lys Arg Leu Ala Leu Lys Ile 335 Arg Leu Asp Ile Glu 415 Gin Ala Tyr Asp Phe 495 Phe Tyr Gly Trp Asp 575 Ala Ser Thr Ser Cys 655 Tyr Arg Gly Trp Trp 735 Tyr Val Tyr 320 Asp Ala Va1 Gly Gin 400 Leu His Ile Va1 Pro 480 Asp Phe Val Pro Cys 560 Leu Ile Met Val Ala 640 Gly Gly Gly ser Ala 720 Tyr lie WO 00/22092 WO 0022092PCT/NZ99/001 69 As n Thr Leu 785 Ile Asp His Asp Gly 865 Thr Al a Phe Leu Val1 945 Thr Ile 770 Ser Val Trp Leu Thr 850 Giu Leu Leu Phe Met 930 Phe Ile 755 Pro Asn Thr Trp Phe 835 Asn Leu Leu Asn Al a 915 Gly Trp 740 Val Al a Leu Ser Arg 820 Al a Phe Tyr Ile Ly s 900 Phe Arg Leu Tyr Ile Al a Val1 805 As n Val1 Thr Met Val1 885 Gly Trp Gin Leu Pro Cys Ser 790 Leu Giu Phe Val Ile 870 Asn Tyr Val As n Ser 950 Phe Leu 775 Val Glu Gin Gin Thr 855 Lys Met Glu Ile Arg 935 Ser Thr 760 Leu Leu Leu Phe Gly 840 Thr Trp, Vai Ala Leu 920 Thr Leu 745 Ser Thr Phe Arg Trp 825 Phe Lys Thr Giy Trp 905 His Pro Ser Giy Arg 25 Giy Glu Cys As n Ile 105 Giy Leu Pro Gly Lys Leu Gly 795 Trp Ser 810 Val Ile Leu Lys Ala Aia Thr Leu 875 Val Val 890 Gly Pro Leu Tyr Thr Ile Ser Gly 955 Cys Phe 10 Ser Arg Ala Asp Gly Lys Ile Ile 75 Lys Asp 90 Arg Leu Thr Val Leu Phe 780 Leu Gly Giy Met Asp 860 Leu Al a Leu Pro Vali 940 Leu Leu Tyr Val1 Asp Gly Gly Ile Vali 765 Ile Phe Vali Giy Leu 845 Asp Ile Gly Phe Phe 925 Val Arg Asp Tyr Pro Lys Val Ile 750 Aia Ile Leu Ser Val1 830 Al a Al a Pro Phe Gly 910 Leu Leu Giu Ser Gin Ile Asn Glu Thr 110 Tyr Pro Ser Ile 815 Se r Gly Giu Pro Ser 895 Ly s Lys Trp Cy s Gly Thr Gly Al a Giu Val Cys Thr Ile 800 Giu Al a Leu Phe Th r 880 Asp Val1 Gly Ser Arg Val1 Glu Ile Lys Al a Ile <210> <211> <212> <213> <400> Leu Lys Asp 1 Val Glu Arg Giu Leu Lys Ser Glu Ile Giy Lys Asn Ile Gly Lys Asp Arg Pro Leu Giu Lys 115 <210> <211> <212> <213> <400> 147 124
PRT
Eucalyptus grandis 147 Ala Ile Ilie Ser His 5 Ser Ile Val Giy Glu Asp Thr Val met Met 40 Ala Ser Leu Leu Ala 55 Thr Lys Ile Arg Asn 70 Asp Val Ala Ile Val Gly Asp Gly Phe Tyr 100 Ala Thr Ile Glu Asp 120 148
PRT
Pinus radiata 148 WO 00/22092 WO 0022092PCT/NZ99/001 69 Asp Thr Ile Ser Asn Gly Gly Leu Gin Arg Ile Tyr Giu Arg Tyr Thr 1 Trp 10 Thr Lys Ile Tyr Giu Lys Leu Leu Aia Gly Phe Trp Lys Leu Giu Met Pro Phe Ser Tyr Vai Ser Lys 1s Val Tyr Giy Phe Arg Tyr Lys Thr Vai Leu 40 Lys Arg Arg Giu Ser Val1 Phe Tyr Ile Vai Giu Giu Leu 55 Ser Tyr Arg Asn Leu Giu Thr Asp Giy Giu Lys Ser 149 <211> 375 <212> DNA <2i3> Eucaiyptus grandis <400> 149 atccgctggg aggtttagga ctgacatcag cgatctcatg acccaaattg ttgagttcct agccaagaac cgacaacttc gatacaatac ggtgggccat caagaatgcc ggccaatact agcgcacctc tcaccgtcaa cccatcgact atgccttctt acgaacgtgt tcgatgccaa ggggacatga ccctcatggt aatatagcta gtgctgttag gggac catttatcca cgatggggcc cttcgacact gggtgaagtg attctacaac ttcctgagtc accccagtcg ctagtatcgg ggatggccta ggtcttatgc tctatggtaa tggacaacgg ctcttaaggc cagatggtga cgaggctcgc 120 180 240 300 360 375 <210> <211> <212> <213> 150 356
DNA
Eucalyptus grandis <:400> 150 gaacaacaac tctccattgc gaaagacatc agcctgccct agcccatgag taccggaatc gagggctggc ggggctgccc cgcgttcgct gcgacggtcg gaagggcggc acgcgaagag tagccaacat acgccctatt tgttcgacgc tcagggtggt acaacgcgag gccgaacggg ctacacctac cacctcgccg gatgctggac ggtctcggag gacatacaac gcgatcgaga ttcagctacg tcggtcgtcg gccctctact agcggctggc gggaatctga cctacatatt ttggtaaccc tccgagacgg ccgcactcga catccgcggg tcaagcacgt cgcctt 120 180 240 300 356 <210> 151 <211> 470 <212> DNA <213> Eucalyptus grandis <400> cttcctcagg gagaccggct caaggacact ctcgattaeg caaacttgtt tacactaaca ggtgctgggc ttcaccgata tgactcgacc caagtcggcg ccgccatgta tcgtcgggcg cgcctttctt cgctgttcaa tgttggacgg tcgagatcgt tggatgcaaa ccggcacgcc ggtgaacccg gccgaatgca gcaattggac gatagccgaa gacaggcggca cctcatgcca tacccatcttt agggtgatgg gctgtctact acaggatggc gaatacaaca aaacggacat tcggcttctc atgagaattc cggcgatgaa cctcattgtg gtaatctcat ttgagaccta WO 00/22092 WO 0022092PCT/NZ99/001 69 catttttgct ctcttcaacg agaatttgaa gccgggaccg acgtgcgaga ggaacttcgg actcttcagg ccggacatga caccggtgta tgatgccggg atcttgaggc ':210> 152 ':211> 412 <212> DNA <213> Eucalyptus grandis 420 470 '400> 152 tgatttattg tgattgccat actctcttat tttaacatca gcacccatta gttgaaatta aatgtttctc gtactccggg cgatcggctt tccgggcttc tgtaaattct cgcgttttca tccgacacaa ccacttctag tcctcttctt ctgggcaggt atagggtact ttcttctggt ttgggagtcc atggacgttc ggaccccccg tcctgattct gtgcccttat ttggctgtct tctcgtcgaa cttgcttcac ccttcccgcc ccggatttct gcatgttaca tccttaatac attgggcgaa catggcaaat gtgctgaatc agcagatggg ctagtgcaga cggtgcagca aaggcagcgt aagaggcccc atgtaagttt aaat tcgagt ccaaccactc gt ':210> <211> <212> <213> 153 328
DNA
Eucalyptus grandis '400> 153 gaacaacaac tctccattgc gaaagacatc agcctgccct agcccatgag taccggaatc gagggctggc ggggctgccc cgcgttcgct gcgacggtcg gaagggcggc acgccgaaga tagccaacat acgccctatt tgttcgacgc tcagggtggt acaacgcgag ggccgaac ctacacctac ttcagctacg cacctcgccg tcggtcgtcg gatgctggac gccctctact ggtctcggag agcggctggc gacatacaac gggaatctga ttggtaaccc tccgagacgg ccgcactcga catccgcggg tcaagcacgt <210> 154 <211> 373 <212> DNA <213> Eucalyptus grandis <400> 154 gttaacgcct gccctcttcc ctcttcgacg atcaagatgg gccagcaagg ggcggcaccc gagaacaaga accccttctt gggagaaccc cccagatcga tcgtcaccga acaatgccgc cictgaggcg agc cgcctacgag gggcgtcgtc cgccgtcttc gacgggctgg cgcctacaac gcaggccgac tccaactccg gacgccggca gccgccatgt ccctccaagg ggcaacctcg ctcaccgtct acgtcatctc acgggctccg cggccctcaa gcgacgagaa tccgccggat acctcttcgc cctggactac ctacttcaac gtacgacgac cgaggtcggc cctcaccggc gctcttcaac <210> 155 <211> 465 <212> DNA <213> Eucalyptus grandis <400> 155 cgatcatcag tttcctggtg tcagctacgt cggtaacccg cggtcgtcgt ccgagacggg ccctctactc cgcactcgag gcggctggcc atccgcgggc ggaatctgat caagcacgtg cctacatatt cgccttgttc agcaacaact aaagacatca gcccatgagt agggctggcg gcgttcgctg aagggcggca gacgagaacc ctccattgct gcctgcccta accggaacct gggctgccct cgacggt cga cgccgaagag agaagcagcc agccaacatc cgccctattc gttcgacgcg ccgggtogtg caacgcgagg gccgaacggg ggagctggag tacacctact acctcgccat atgctggacg gtctcggaga acatacaacg gcgatcgaga aagcacttcq WO 00/22092PC/Z90I9 PCT/NZ99/00169 ggctcttctt ccccaacaag cagcccaagt acccgctcag ctttg <210U> <211> <212> <213> 359
DNA
Eucalyptus grandis <400> 156 ccagatcaag gtctcaacag taagggcaca cttaaggccg caacaacaag tcccctctgc caacgaacgc ctcgactatg acttgggtac cggaacttgt atccggcggg gggtccctag ctgtggacac atcacagacc ttgtcaacat ctctgttcac ttgacgcaat aagtggttgt qcgcatctta tctcctagat ctatccgtac ggcgaactcg tctagatgct gtcggagagt ggagaatcgt cctataatca ttcagctaca gttgtggtgt gtttactctg ggttggccat ctcctccatc Cattcctagt gcgacaatcc cgga tgg ag9c cactagagaa Ctgctggcg <210> 157 <211> 325 <212> DNA <213> Eucalyptus grandis <400> 157 ccagatcaag taagggcaca caacaacaag caacgaacgc acttgggtac atccggcggg gtctcaacag cttaaggccg tcccctctgc ctcgactatg cggaacttgt gggtccctag c tg tgg aca c atcacagacc ttgtcaacat ctctgttcac ttgacgcaat aagtg gcgcatctta ggagaatcgt ctcctccatc tctcctagat cctataatca cattcctagt ctatccgtac ttcagctaca gcgacaatcc ggcgaactcg gttgtggtgt cggatggagc tctagatgct gtttactctg cactagagaa 120 180 240 300 325 <210> 158 <211> 362 <212> DNA <213> Eucalyptus grandis <400> 158 gtttgctatg ggatgctcgg aagtcccgcg gcatcacgca ctgagaggct cgaacatcga gcttccaacc ccgccaacgc ggcgtcaggt tccgctacat gcacaattcg ccaggttcgt tc gaacaacctg gatgagactc gctCatcctc gaactcgtgg cgccgtgggc cctccccgcg CCgtCCgCgt tacgacccta ggcgtcccca gtgcagagga aacgaggtga atgaggaaca cggaagtcgt gccaaccagc actcggagct acgtgaagaa gccctgtcaa tacaggccgc cgccctctac tctacaagcc ccaggccctt Ctactcgccc cggaggcact gctcagatcg <210> <211> <212> <213> 159 432
DNA
Eucalyptus grandis <400> 159 taaatgccga tgtataCgaa gaagcgacat. aagtgatgtc ccttcaccgt gaac.attLac attatgcttt cttcgacggt tctttgatgc caactttgaC tgaccatcat cataggcgag gtaacgcgta caggttttat Cacttcggcc ag tcaccagaag atgacccaaa ccgtttCtga gcaactccaa actttogtgt gtgggttggc aatoggctct acaacccact tcgtcaagtt ttgttgacaa cggctcttaa ccacagaagg ttacaaaact tCCatccgcc catggctgag caataatgac ggggattgag agcagttgga tgacataaat tggagcgaat ggaacgttcc aacaatgcac ctccctcuCa tacaccaatg catgggaaca gcaaacaacg agagggactc WO 00/22092 WO 0022092PCT/NZ99/00 169 <210> 160 <211> 379 <:212> DNA <213> Eucalyptus grandis <400> 160 caccgtacct gtgaacgcag tgggaggttt aggactgaca gaacagcgca cctctcaccg cttccccatc gactatgcct atacacgaac gtgttcgatg ccatggggac atgaccctca tgccaatata gctagtgct acgtgtacaa tcagcgatct tcaacattta tcttcgatgg ccaacttcga tggtgggtga ctcccccgtc catgacccaa tccattcctg ggccacccca cactctagta agtgggatgg agcaatcccq attgtcgagt agtctetatg gtcgtggaca tcggctctta cctacagatg taccatccgc tcctagccaa gtaacgacaa acgggataca aggtggtggg gtgacaagaa 120 180 240 300 360 379 <210> 161 <211> 361 <212> DNA <213> Eucalyptus grandis <400> 161 gtttgctatg ggatgctcgg aagtcccgcg gcatcacgca ctgagaggct cgaacatcga gcttccaacc ccgccaacgc ggcgtcaggt tccgctacat gcacaattcg ccaggttcgt t gaacaacctg gatgagactc gctcatcctc gaactcgtgg cgccgtgggc CCtCCCC9Cg ccgtccgcgt tacgacccta ggcgtcccca gtgcagagga aacgaggtga atgaggaaca cggaagtcgt gccaaccagc actcggagct acgtgaagaa gccctgtcaa tacaggccgc cgccctctac tctacaagcc ccaggccctt ctactcgccc cggaggcact gctcagatcg <210> 162 <211> 402 <212> DNA <213> Pinus radiata <400> 162 ggactacgct ctcttccggc cgacaacctg ttctacgccc cggtaaggtc gaggtcaggg gctcggtgcc acgcccgaga caagaaggag ggtactcccc ctttaatgag aatttgaagt agacggaacc gagacgtatg ccagcaatgg agatcgacgc tctcggagac atgcaaagac tgaagcccaa ccgggcctac accttggctt cgtggtcgat tgcgtactcg agggtggccc ttacaatggg tgtgagcgtg atccgagaga gaaagggatt tccaagacca gcgctcgccg tccaaggggg aaccttttgg caggccttca aattatgggc ga atttgcacta ctctgggcta acgatgatga agaggctcca tttttgcgct tctttaaacc <210> 163 <211> 297 <212> DNA <213> Pinus radiata <400> 163 ccctcgtcga tacccttctt tctgccatgg aggacttggg gtatcgcaac atcccactca tcgttactga aagcggatgg ccttctggtg gcaatgatgt ggccacggtt gacaacgctc qCqtttataa caacaaccuc acccgccatg tgctctcaaa tgtagggact cccaagaggc cgggaacgag cattgagacc tacatcttcg cacttttcaa cgagaacaga aaagctggtg atgagacgga gcgtcacttt gggcttttct accctaacca acaatttgta tactctg <210> 164 <211> 427 <212> DNA WO 00/22092 WO 0022092PCT/NZ99/00I 69 <213> Pinus radiata <400> 164 gcttcccacc gtctaaaggt ttcaattcct gtcagatcac acaatgggaa caggggttcc tggtgcagga cgagggtcgc tttctgccat ggaggacttg ggccttctgg tggcaatgat tcatccgcca tgtgctctca cctacat gtcttcagga gggtctccct atttctctgg agctacatca gggtatcgca gtggccacgg aatgtaggga acgaggttaa tcatggctaa actacgctct acttattcga acatcccact t tgacaacgc ctcccaagag agatatcatg catctatcca gtttaggtca tgccctcgtc catcgttact tcgcgtttat gccgggaacg agttctctac tacttcagct acctctaccg gatacccttc gaaagcggat aacaacaatc agcattgaga 120 180 240 300 360 420 427 <210> <211> <212> <213> 165 205
DNA
Pinus radiata <400> 165 ggttgacaac gctcgcgttt ataacaacaa tctcatccgc catgtgctct caaatgtagg gactcccaag aggccgggaa cgagcattga gacctacatc ttcgcacttt tcaacgagaa cagaaaagct qgtgatgaga cggagcgtca ctttgggctt ttctacccta accaacaatc tgtatactct ctaaacttta ctccg <210> 166 <211> 393 <212> DNA <213> Pinus radiata <400> 166 ggactacgct ctgtttaggt caacttattc gatgccctcg caacatccca ctcatcgtta ggttgacaac gctcgcgttt gactcccaag aggccgggaa cagaaaagct ggtgatgaga tgtatactct ctaaacttta caacctctac tcgataccct ctgaaagcgg ataacaacaa cgagcattga cggagcgtca ctccgtaact cgtggtgcag tctttctgcc atggccttct tctcatccgc gacctacatc ctttgggctt 9cg gacgagggtc atggaggact ggtggcaatg catgtgctct ttcgcacttt ttctacctta gcagctacat tggggtatcg atgtggccac caaatgtagg tcaacgagaa accaacaatc <210> 167 <211> 394 <212> DNA <213> Pinus radiata <400> 167 ggactacgct ctgtttaggt caacttattc gatgccctcg caacatccca ctcatcgtta ggttgacaac gctcgcgttt gactcccaag aggccgggaa cagaaaagct ggtgatgaga tgtatactct ctaaacttta caacctctac tcgataccct ctgaaagcgg ataacaacaa cgagcattga cggagcgtca ctccgtaact cgtggtgcag t ctt t Ctg cc atggccttct tctcatccgc gacctacatc Ctttgggctt 9 cgt gacgagggtc atggaggact qgtggcaatg catgtgctct ttcgcacttt ttctacctta gcagctacat tggggtatcg atgtggccac caaatgtagg tcaacgagaa accaacaatc <210> 168 <211> 498 <212> DNA <213> Pinus radiata <400> 168 WO 00/22092 WO 0022092PCT/NZ99/0OI 69 ggnnacgctc aacttattcg aacatcccac gttgacaacg actcccaaga agaaaagctg gtatactctc tatgaatatg gcagctcata tgtttaggtc atgccctcgt tcatcgttac ctcgcgttta ggccgggaac gtgatgagac taaactttac tcctctatat tgcttcta aacctctacc cgataccctt tgaaagcgga taacaacaat gagcattgag ggagcgtcac tccgtaactg gtcaactgcc gnggtgcagg ctttctgcca tggccttctg ctcatccgcc acctacatct tttgggcttt cgtcgcagtc tcgatagata acgagggt cg tggaggact t gtggcaatga atgtgctctc tcgcactttt tctaccttaa cgacgaacga tattatgtaa cagctacatc ggggtatcgc tgtggccacg aaatgtzaggg caacgagaac ccaacaatct atagagccaa catgctcgat 120 180 240 300 360 420 480 4.98 <210> 169 <211> 278 <212> DNA <213> Pinus radiata <400> 169 ggactacgct ctgtttaggt caacctctac aacttattcg atgccctcgt cgataccctt aacatcccac tcatcgttac tgaaagcgga gttgacaacg ctcgcgttta taacaacaat actcccaaga ggccgggaac gagcattgag cgggtgcagg acgagggtcg cagctacatc ctttctgcca tggaggactt ggggtatcgc tggccttctg gtggcaatga tgtggccacg ctcatccgcc atgtgctctc aaatgtaggg acctcatc <210> 170 <211> 419 <212> DNA <213> Pinus radiata <400> 170 ggactacgct ctgtttaggt caacttattc gatgccctcg caacatccca ctcatcgtta ggttgacaac gctcgcgttt gactcccaag aggccgggaa cagaaaagct ggtgatgaga tgtatactct ctaaacttta c aacctCt a c tcgataccct ctgaaagcgg ataacaacaa cgagcattga cggagcgtca ctccgtaact cgaggtgcag tctttctgcc atggccttct tctcatccgc gacctacatc ctttgggctt gcgtcgcagt gacgagggt c atggaggact ggtggcaatg catgtgctct ttcgcacttt ttctacctta ccgacgaacg gcagctacat tggggtatcg atgtggccac caaatgtagg tcaacgagaa accaacaatc aatagagcc <210> 171 <211> 437 <212> DNA <213> Pinus radiata <400> 171 gacgtacgga tcgtatcttg caatctgcag aataacatca cccaccgtct aaaggtgtct attcctgtca gatcacgggt tgggaacagg ggttccattt gcaggacgag ggtcgcagct tgccatggag gacttggggt ttctggtggc aatgatg ttcccgccat aggtctcaac tcaggaacga ctcccttcat c tc tgga c ta acatcaactt atcgcaacat gaggaacatt cactcactcc ggttaaagat ggctaacatc cgctctgttt attcgatgcc cccactcatc caaacagcga tcgggcgtta ateatgagtt tatccatact aggtcaacct ctcgtcgata gttactgaaa ttgaaaacgt ctaatggctt ctctacttca tcagctacaa ctaccgtggt ccttctttc gcggatggcc <210> 172 <211> 343 <212> DNA <213> Pinus radiata <400> 172 WO 00/22092 WO 0022092PCTINZ99/001 69 gacgtacgga caatctgcag cccaccgtct attcctgtca tgggaacagg caggacgagg tcgtatcttg aataacatca aaaggtgt cz gatcacgggt ggttccattt gtcgcagcta ttcccgccat aggtctcaac tcaggaacna ctcccttcat ctctggacta catcaactta gaggaacatt cactcactcc ggttaaagat ggctaacatc cgctctgtta ttcgatgccc caaacagcga ttgaaaacgt tcgggcgtta ctaatggctt atcatgagtt ctctacttca tatccatact tcagctacaa ggtcaacctc taccgtggtg tcg <210> 173 <211> 563 <212> DNA <213> Pinus radiata <400> 173 ctggattatg accaacctgt cccaacatcc accattgaga ggaacaccaa gatttgaaag cctgtatact cgagtcgatg ggttatttct aatgcaggca ctctgtttaa tcgatgccat cgatcgtcat atgctcagag agaggccagg gcaacgagac ctgtcaactt cagacaagcc aaaataaata gagttccctg gtctacgtct ggttgacact tgccgaaagt ctataacaat aatgagcatc agagaaacac ctcaccatga cttatcttca aaatgtcaaa agt acqgtggtgc cttttgtcgg ggatggcctt aatcttatta gacacatatg tttggactat gttgtgttgg tgcactgcta gaaagttgag aagacggtga ccatggaagc ctgctggcgc aacatgtatt ttttcgcgct tcgaccctac aatccacatc tatgtaatct tttatttcta tcacagctac ctcggggtat ggatctggcc atcgaatgca tttcaacgag tactaaacag acatgtgacc gcaagtgtat aaataaataa 120 180 240 300 360 420 480 540 563 <210> <211> <212> <213> 174 639
DNA
Pinus radiata <400> 174 caacgaattg cattcgcccc gataaaaacg gcagaacgcc cgagggctac tgtgcttcaa cagctacatc tacagtggtg cttgtttcgg ggatggcctt aatctcatcc cagctcatct ttctatcccg acgtacggat aatctgcaga ccgccgtcta ttcctgttag aacaacaggg caagacaaag ccatggaagc ctgctgaggc gccatgtctt cctccagcca ccaccaatat cgtatcttgt ataacatcaa acggtgtgtt atcacgggtc ctcagataac gtcggagttc cttgggatat ggatgtggcc atcgaatgaa ggacgccgcg caagtatatt tcctgccatg ggtctcgacc caaagacgag tcccttcatg tctggactac aaatacttgt cccaacatcc acagttgaca gggacacca aatggttggg gcagtaggca aggaatattc actcactcct gtcaaggaca ggcaacatct gctctgttca tcgatgcctt cactgatcgt atgctcgtac tcaatgacaa acgaggtttt aaaccgcgct ccgatgttag ccatgaagtc atccatactt aatccacgtc ggtcgatact taccgaaagc ttataacaac 120 180 240 300 360 420 480 540 600 <210> 175 <211> 534 <212> DNA <213> Pinus radiata <400> 175 aaatggttgg gtgggcaacg aacattcaaa gccttcaatg aaggatacca aatgtgtatc ctctttcagc gatgccatgg cttattgtta gttaatgaca aggttttgcc ccgcgatcca t tat aggca a tgagctctat catatttcag ccacggctac tggattctat ctgaaagcgg atattgtccc tagcacgcag gaacgccaac cagttacccg actcaaattt ctacgtcggc ggcggtgaca tttctccgca atggccatct ctatctatca tacgtarcgt ctgcagaaca ccttcacagg ctgtcagata gacagcagca gacggagatc atggaagcct gctggcgcgg ccagtatcaa atcttgttcc rcaaggtctc gagcattcag acggggctcc acattcatct acagctacag tgggatattc atgcggccac atatattgcg tgccatgaac taccccccat tgacgatgtg gttcatggcc ggactacgct caatttgttt caacatccca aact WO 00/22092PC/Z/016 PCT/NZ99/00169 <210> 176 <211> 345 <212> DNA <213> Pinus radiata <400> 176 ggttaacgag cacattgtgc aaacgaggtt ttgataggcg cattcaaact gcgatccaga caggccggat gttagcagcg ggacacgatg ggccaaatac tgtctatcca tacttcagct ccttctatcc atgccaacaa acgctaaact gctacccgcc tcaaatttct acatcagcaa cgccaccaat cgtaccctat gcaggatagc gtctaaagga gtcacagaac cccaagcaac gtcaaataca cttgttccgg atcaaggtct gtcttcgtag ggcggtccct attca ttgctgtggg ccatgaacaa ctaccaccca atgctgtgaa tcatggcgga 120 180 240 300 345 <210> 177 <211> 339 <212> DNA <213> Pinus radiata <400> 177 cccgatcgtc attaccgaaa gaatgctcag acctataaca aaagaggcca ggaatgagca gcaaggcgac gagacataga atactctgtc aacttctcac cgatgcagac aagcccttat gtggatggcc ataatcttat tcgacacata aacactttgg catgagttgt cttcatgcac ttctgctggc taaacatgta tgttttcgcg actattcgac gttggaatcc tgctatatq gcggaagtgg ttatcgaatg cttttcaacg cctaatacta acatcacatg ccaccattga caggaacacc aggatttgaa aacagcctgt tgacccgagt 120 180 240 300 339 <210> 178 <211> 313 <212> DNA <213> Pinus radiata <400> 178 gtcataactg agagcgggtg gccttccgcg ggcaatgagg cggctactgt cagacttaca acaacaatct gatcaaacat gtgctttcaa atgcaggaac cctggacagc acattgatac atacattttc gctcttttca acgagaattt gacgagccag aacgacattt tggacttttc tatcctgatc aaaaccttgt aacttctccc cttaaattca tctgatctgt gttttgcatt agaatttcca ttttctcaca ata tgagaatgcg gccaaagagg gaaaggcggg ttaccctgtt gtattaccca 120 180 240 300 313 <210> <211> <212> <213> 179 460
DNA
Pinus radiata <400> 179 cgacaacatt cgccaattct agttttttca agtgaaaatc tcaaaccgca gtccagaacg cacgtctgtt ctgggaaact gagtagcatg agcgatgtac cgtgtatccc tacttcagtt gtttaaatcc gcggatcccg tgcgctggtg gattctctga atccggccac ggcagcatct ccaatctgca cgtatccccc ttaactttct acatttacaa tggtgagtga tttccgctat caacatcaaa tccatatctc gagctccata ttctcaggga ggcagagaat ccaggcccaa tgaaggtcgt ggagaaatcq tacatcgctg gttcctgcca aaggtctcca gaattcgctg gggtctcctt atctcgttag ctctataaaa ttggcaacga tgagaaacat ctacccacgc atgaattgaa tcatggctaa actatgcttt gcttgttcga 120 180 240 300 360 420 460 <210> 180 <211> 296 WO 00/22092PC/Z/0I6 PCT/NZ99/00169 <212> DNA <213> Pirius radiata <400> 180 ttatggcggc gtctatccat acttcagcta catcggcaac ttatgctctg tttaagtcta cgtctacggt ggtgcaagac cctgttcgat gccatggttg atactctttt gtcggccatg catcccgatc gtcattaccg aaagtggatg gccttctgct tgagaatgct cagacctata acaataatct tattaaacat acaaaagaca ggtgatcaca gaagcctctg ggcgcggaag gtattatcga tttctctgga gctacaccaa ggtatcccaa tggccaccat atgcag 120 180 240 296 <210> <211> <212> <213> 181 351
DNA
Pinus radiata <400> 181 tacgtaccct atcttgttcc ctgcaggata gcatcaaggt ccgtctaaag gagtcttcgt ctgtcacaga acggtggtcc aacacaaaag acatttctct gacggtgatc acagctacac ggccatgaag ctctaccacc agatgctgtg cttcatggcg ggattatgct caacctgttc aacattcaaa cacaggccgg aaagacacga gacgtctatc ctgtttaagt gatgccatgg ctgcgatcca atgttagcag tgagccaaat catacttcag ctacgtctac gtgatactct gaacgctaaa cggctacccg act caatttt ctacatcggc ggtggtgcaa t <210> 182 <211> 457 <212> DNA <213> Pinus radiata <400> 182 caatcttcca tctccagacg gagaatttac caggaaaacg aatagtgggt gtcgctaacg ccgttgggtt aacgagcaca agtgggaaac gaggttttga ggccatgaac aacattcaaa ctctaccacc cacaggccgg agatgctgta aaggacacga aggtggtaac atgttgtact aagaactgaa ttgtgccctt aaagcttgga ctgcgatcca atgttagcag tgagccaaat tttgatgaag gcaagctttc gaacatatct ctatcccgcc caacgttcag gaacgctaaa cggcaacctg actcaat tccaacaaca gcgaattctg tccagccaag accaatgtca tacataccct ctgcagaata ccgtctgaag ttgggaaaac gtatcgatgt actcggcaaa aatacattgc atcttgttcc gcatcaaggt gagtcttcat 120 180 240 300 360 420 457 <210> 183 <211> 358 <212> DNA <213> Pinus radiata <400> 183 ctttgatgaa gtccaacaac tgcaagcttt ggcgaattct aggacatatc ttccagccaa tctatcccgc caccaatgtc ttcagtacgt accctatctt ctaaactgca ggatagcatc attgggaaaa ggtatcgatg gactcggcaa aaatacattg gttccggcca aaggtctcta cgagaattta taatagtggg accgttgggt cagtgggaaa tgaagaacat ccacccacag ccaggaaaac tgtcgctaac taacgagaac cgaagttttg tcaaactgcg gccggatgtt aaagttgtac agcgaactgg attgtgccct ataggcaacg atccagaacg agcagcgg 120 180 240 300 358 <210> 184 <211> 348 <212> DNA <213> Pinus radiata <400> 184 WO 00/22092 WO 0022092PCT/NZ99/00I 69 cgtaccctat ccaggatagc gtctaaagga gtcacagaac cacaaaagac cggtgatcac cttgttccgg atcaaggtct gt-cttcgrtag ggtggtccct at tt ct ctgg agctacacca ccatgaagaa ctaccaccca atgctgcgaa tcatggcgga attatgctct acctgttcga cattcaaact caggccggat ggacacgatg cgtctatcca gtttaagtct tgccatggtt gcgatccaga gttagcagcg agccaaatac tacttcagct acgtctacgg gatactct acgctaaact gctacccgcc tcaatttttt acatcggcaa tggtgcaaga 120 180 240 300 348 <210> 185 <211> 594 <212> DNA <213> Pinus radiata <400> 185 aactttgatg aagtccaaca actgcaagct ttggcgaatt gaggacatat cttccagcca ttctatcccg ccaccaatgt gttcagtacg taccctatct gctaaactgc aggatagcat tacccgccgt ctaaaggagt aattttctgt cacagaacgg atcggcaaca caaaagacat tgcaagacgg tgatcacagc gcattgggaa ctggtatcga agactcggca caaatacatt tgttccggcc caaggtctct cttcgtagat tggtcccttc ttctctggat tacaccaacc aacgagaatt tgtaatagtg aaccgttggg gcagtgggaa atgaagaaca accacccaca gctgtgaaag atggcggacg tatgctctgt tgttcgatgc t acc agg aa a ggtgtgctaa ttaacgagaa acgaagtttt ttcaaactgc ggccggatgt acacgatgag tctatccata ttaagtctac catggttgat acaaagt tgg cagcgaactg cattgtgccc gataggcaac gatccagaac tagcagcggc ccaaatactc cttcagctac gtctacgggg actc 120 180 240 300 360 420 480 540 594 <210> 186 <211> 360 <212> DNA <213> Eucalyptus grandis <400> 186 cgacatgttc gacaaggccg gcaggtcggt acccagactg tgctggtgga tgtaccaatg ggtccattcc atcaacggta ctgtccggat gacacgactt cacttatggc attactactt ctctcttcgc ctgaatctca tttctggtgg ctactaactg gcgcagccaa ccggcaatgc tttctcttta tccgcctctt tagtgttgtc ttacactggt ctgcgctttg tcttaccctc ctcgccgtca acctggcaaa attgacgcga caagcatgga gatggtgctq aagttcgtca cttacggtca aatgtacgac attggcgttg acacgacact attactctgg ctcaatcttc <210> 187 <211> 397 <212> DNA <213> Eucalyptus grandis <400> 187 atccacccta gcgagcagga ccgacttttt tgccctctga ctttggccat tgttccatta cggtccttgt ggcaggctta gtgattaatc cagaggatga actttcttga ggaaaaggtt ccctcttcag agatttacaa tgaggtctct tttgtttact catgttgcct tgt c tccgt c tttcgtttgg caatagagtg gactctgccq caaatactac agatactatc ttgtcgcctg aataagattt gtacacgatt aagcttggct atcaggg ttgaaacttt atgggttctg acctcggtgg tcgcagatag accatttgat tcttccttca tagatgtgtc caagcaacag tcggttcaac gatcatggaa ggtgttgccg cagcccattc <210> 188 <211> 531 <212> DNA <213> Eucalyptus grandis <400> 188 WO 00/22092 WO 0022092PCT/NZ99/00 169 ggaagacaaa ctatcagtaa caatgaacac cttcttgcaa cagccgagtg ctataatgaa ctttggtgtg tgttggctca aaattgtgga gagaagagct aatgccaggg tgtatttatt gaagcttgga ggaaaccagt ttcatacaca gcaccatcag tctagagagt ggtcaaacct atatttctgg cctgaagtcc atcagtgggc atagccgctg tcattgtctg gaggcaactg gatgcagacc gtgctcgcaa cagggagtaa attatgacgg tttctgtttt gggggagaaa agcatgggta ctgatcttga atggctcaag ctgattttgg atgaacctgc gcaaaggat t cactgttgtt gaacgctct c at cat taagt ttttataagg ttggaagaat catagagtac at catgccaa agttgttaag ggttgcagag cctgaaactt tgtaatgatc gagtggcttt tggaacagtq accgtggaac aaggagagcg gccaaggaat aggggacagc a 120 180 240 300 360 420 480 531 <210> 189 <211> 329 <212> DNA <213> Eucalyptus grandis <400> 189 atcctccaga aatcagcttt cttgagaaac gagactacgt ccgcccaaca gcgcatgaga tttatccgga gatgacctcc gtttggtaaa ggtcgttgtg aagcttcaat gaagatcgtt tgttgtctta cgtcagagga aatagttttc tcccactacc tcttcaccgc tctcccctg ctcctcaaaa tgtaagcatg CCCcttgaga actggcatgg agacgctttt agtgaggcac tagcgagcat tcgagcacat ttgttagttc gacccgcttt ttgccagttc t tgtacgggt tccaagatat ttttctcagg tactagcttg <210> <211> <212> <213> 190 503
DNA
Eucalyptus grandis <400> 190 accaaaagcc tccatcagtg cccaagaatg tcatcgtgtg gaaggagat t ccggttatga tcaattgtgt cttttagatc cacattgtgg gagcgattct tgatacctac ttgtcttcct atagacttgg gggaaacttg gattatacat ggaactacca acctggaaag aagttaaacc tttggattat tgctgagaca tgtcagtgga gttagcagca tgtttcggtg ggaaacaact gtgcgcagat tgttcttgca t ca gatggagcta gttggactca aaggagcgag gagcatggct acagattttg gatggctcta Ccagattttg aatgaacctg tggggtcaac ttaacagttt ttatcctaag attttcttag actggaaaca ctatagaaat gtttttgcca tttctgtcaa aggatccaat gtgccgagat taaatggttc gccaaaccaa gacagctgag caaagagagt ggcaaaggaa gagtggccaa <210> 191 <211> 398 <212> DNA <213> Eucalyptus grandis <400> 191 acgacactag aggatccaaa actatgtctq gatacatgat tcatcaagct gaggatgggg ggactcttcc agnnaaggag tccacacgtt cgattatgct agtaccagtc gaaaaggggt agatcatgcc tgttgggatc gtctcgcaaa tatcattgga ttctttctcc gagatactca cggcattttc tatatcggnt cacatgggcc aagggattga gggggttgcc atagcccttt aagcgcttct tatcctgrtg tggaatatta agattcag ggtcataaat tactttctta tccatcatca taatgctgac cagtagaatc tggaagaaca ccagaggatg agaaggaagt gagatatata ctaattggtt tggggngtgg attggggtaa <210> 191 <211> 457 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00 169 <400> 192 ggcaggctta tgtctccgtc cagaggatga tttcgtttgg ggaaaaggtt caatagagtg agatttacaa gactctgccg tgatagggtt ccacactttc ttggtcttac ctatgaatcg ttagtatcaa aattcttcca ttcccgagac tgaagccaag aataagattt gtacacgatt aagcttggct at canggagg gactatgcac aagaggggtt gttgggatac gtggccgaac tcgcagatag accatttgat tcttccttca agcttctgan gccatttcct atataggcct a cat ggg a ca taattaa gatcatggaa ggtgttgccg cagcccattc ggctttcctg gtcttgttgt ggactattat attgcagtcc gtgattaatc actttcttga ccctcttcag aattctgatt agtcggatgc ggtcggactg gttttaagcc <210> 193 <211> 359 <212> DNA <213> Eucalyptus grandis <400> 193 gcctgacctc ggtggtcggt gattttcgca gataggatca cgattaccat ttgatggtgt tggcttcttc cttcacagcc ggaggagctt ctgagggctt tgcacgccat ttcctgtctt tcaaccggtc tggaagtgat tgccgacttt cattcccctc tcctgaattc gttgtagtcg cttgtggcag taatccagag cttgaggaaa ttcagagatt tgatttgata gatgcttggt gcttatgtct gatgatttcg aggttcaata tacaagactc gggttccaca cttacctatg ccgtcaataa tttgggtaca gagtgaagct tgccgatcag ctttcgacta aatcgaaga <210> 194 <211> 401 <212> DNA <213> Eucalyptus grandis <400> 194 ttggcttctt. ccttcacagc gggaggagct tctgagggct atgcacgcca tttcctgtct ggggttatat aggcctggac ggatacacat gggacaattg ccgaactaat taagcagttt tggatatttt taaaggaatt ccattcccct ttcctgaatt tgttgtagtc tattatggtc cagtccgttt ggtggtcggg agcttgaaac cttcagagat ctgatttgat ggatgcttgg ggactgttag taagccttcc gtaggacaat tgttggccat ttacaagact.
agggttccac tcttacctat tatcaaaatt cgagactgaa gttgctcggt g ctgccgatca actttcgact gaatcgaaga cttccagttg gccaaggtgg gtagatgaca <210> <211> <212> <213> 195 364
DNA
Eucalyptus grandis <400> 195 ccgactttct tgaggaaaag ttcccctctt cagagattta ctgaattctg atttgatagg tgtagtcgga tgcttggtct tatggtcgga ctgttagtat tccgttttaa gccttcccga ggt c gttcaataga caagactctg gttccacact tacctatgaa caaaattctt gactgaagcc gtgaagcttg ccgatcaggg ttcgactatg tcgaagaggg ccagttggga aaggtggccg gcttcttcct aggagcttct cacgccattt gttatatagg tacacatggg aactaattaa tcacagccca gagggctttc cctgtcttgt cctggactat acaattgcag gcagtttggt <210> 196 <211> 400 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/00 169 <400> 196 gttttaatgc aacaatgact tcaaagagat gaagctcctt aggatccaaa aactactata attttggcga actcgacatg aaggagaatg gatgatcaca agcttgtttt tgattatttc ctctttagtt tggacctata gcacaagtto ttgcatcctg gttgtgctta tggttagcag atgccagaat aaagaaaggc agtatgcaga atacacctgg atctgggtga gcggaagtga cagaaaatgg atcttaatat acctcgatcg tgttgaattt tagaagaggt aaccctttca aagaaatgtc tatgtttctg ggactggatg tatgtngagg catgatcaaa gttttatgta ttagatgaga cgccatacga gagagtgtaa ttcgagaaac 120 180 240 300 360 400 <210> 197 <211> 298 <212> DNA <213> Eucalyptus grandis <400> 197 taaaaccgnt agcagcaagt gtttgtatgg tttctcgcaa agagaccctt catattgtcg ggaccggaga taaccaaggn atttcggcat attcggagtg gctgtccatt tcaattgcta cgctcgacgt aattgggaaa ccgatggtcg a caa tggtg t ctcacaaagc acgtgggct c atctgaagta gtgcagacat attggagtat actccaaggg cgggaagtat ttccatctcc atgcgggttc gatgctcaca ctccagggaa gtngctcact actatgctga taccccaa 120 180 240 298 <210> 198 <211> 402 <212> DNA <213> Eucalyptus grandis <400> 198 ctggaccgga gataaccaag gaattttggc atattcggag cccgactgag gagctttgca tnggggggnn ttncccnntt ccgaatctgc taggaatgct tcaattacca agctcatacg ccgattacgt ggagagttac gcaattggga tgccgatggt accgctggat tttttttncc cttggcatga acgggagccc ggattgagca aaatttgaag cggtgcagac tgaaagtcgg cnaaaagggg gatataggct cgattgcacg cccagttttt tattccatct atatgcggct tgccttntac tttttaatgg cctaccttac gcctcttttc gc ccactatgct tctacccggc ccnttntttt gactcagtag ttgtacactc ttttcattcc 120 180 240 300 360 402 <210> 199 <211> 441 <212> DNA <213> Pinus radiata <400> 199 atcgacccag ggattggcgt gacgttttca taaagcacga tactttccgg acttcctgaa ttccaccaaa tggtccccgt tqcaqtqqca agtgctccat gactgctgcc tcgactgcac atcaacgcct ccggtaccca cactacaacg gcgtcctcga taacacgagc cgggagtccg cccaaagacg ggacggtctc ccccacaaac aaacatcacc ggtcccgctg 9 tacgggacgt ttcttgggtc gtggatttct tggatcgaca cggagctgcc gccacccgat gggttcaaga tccagcgagg aggtgtggcc gggccgacga tgaacgaggt cgggcacggg gggacgtgcc ccatcgccac aatggcggac cggcgccgtg gatctcccgc ctccaatttc tttcccatgg accctaccag cagctccgtc <210> 200 <211> 481 <212> DNA <213> Pinus radiata <400> 200 ctataacaaa tctcagatag ctctggatgt tatatggaac gatgatgacc acatggatgg WO 00/22092 WO 0022092PCT/NZ99/O01 69 agccaaagac tgaccgaatt taacacgagc cgggagtccg cccaaagacg ggacggtctc ccccacaaac ttcacccttg catgccaatg tacgggacgt ttcttgggtc gtggatttct tggatcgaca cggagctgcc accctatcaa gaatgagata tccagcgagg aggtgtggcc gggc cgacga tgaacgaggt cgggcacggt ctatcctgaa tgtcgtcctt aatggcggac cggcgccgtg gatctcccgc ctccaatttc ttcccatggg tataagctgc atcgacccag gacgttttca tactttccgg ttccaccaaa tgcagtggca actgctgcct gtcccttcct ggattggcgt taaagcacga acttcctgaa tggtccccgt agtgctccat cgactgcaca 120 180 240 300 360 420 480 481 <210> 201 <211> 484 <212> DNA <213> Pinus radiata <400> 201 ggtggtgcgg accataagca gttggtatgt gagatattgc ctttttacgg cgaggacgcc cttgcccgcc caacttgagc agcg tgttcaaagt tcacggttcc tcgccgagac tcctcaaaca acaacgctga gtgaccttgg gaatcaccaa tgctgaacgt agactggatg aaatagtcct catagatgtt caagcgtgct acgagaacgg atgttggacg tcttgacatg cactgccgta agctccgtac agtagtgttg cgcgttatta acccttggtg gctcggaaca ggggaaaacc aactcgagac gatcccacag aagttggtac gaaggatcga gggacgacgg tcgaacaaca tatttgaaga tcctcgggga tcgtcttcgt gtagcggcga caggagcaac ggcggaaagt tcttaagtcg cctcgccaga cgatatctgc cttcatatcg acgtaacctg gagtcaggtc <210> 202 <211> 418 <212> DNA <213> Pinus radiata <400> 202 caatgcaggt ggggatacaa aaatctcaga tacctctgga gacttcaccc ttgaccctat attcatgcca atggaatgag agctacggga cgttccagcg ccgttcttgg gtcaggtgtg acggtggatt tctgggccga gaacgtatca tgttatatgg caactatcct atatgtcgtc aggaatggcg gcccggcgcc cgagatctcc gacataacga aacgatgatg gaatataagc cttatcgacc gacgacgttt gtgtactttc cgcttccacc atgtggtgga accacatgga tgcgtccctt cagggattgg tcataaagca cggacttcct aaatggtccc aaactataac tggagccaaa ccttgaccga cgttaacacg cgacgggagt gaacccaaag cgtggacg <210> 203 <211> 395 <212> DNA <213> Pinus radiata <400> 203 caatgcaggt ggggatacaa aaatctcaga tacctctgga gacttcaccc ttgaccctat attcatgcca atggaatgag agctacggga cgttccagcg ccgttcttgg gtcaggtgtg acggtggatt tctgggccga gaacgtatca tgttatatag caactatcct atatgtcgtc aggaatggcg gcccggcacc cgagatctcc nacataacga aacgatgatg gaatataagc cttatcgacc gacgacgttt g tgtactttc cgctt atgtggtgga accacatgga tgcgtccctt cagggattg9 tcataaagca cggacttcct aaactataac tggagccaaa ccttgaccga cgttaacacg cgacgggagt gaacccaaag <210> 204 <211> 390 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 204 cgtccttatc gacccaggga ggcggacgac gttttcataa cgccgtgtac tttccggact ctcccgcttc caccaaatgg caatttctgc agtggcaagt cccatgggac tgctgcctcg ctaccagatc aacgcctcgg ttggcgttaa agcacgacgg tcctgaaccc tccccgtgga gctccatccc actgcacaaa tacccaagtc cacgagctac gagtccgttc aaagacggtg cggtctctgg cacaaaccgg catcaccgcc ggcacgttcc ttgggtcagg gatttctggg atcgacatga agctgcccgg acccgatggg agcgaggaat tgtggcccgg ccgacgagat acgaggtctc gcacgggttt acgtgccacc <210> 205 <211> 245 <212> DNA <213> Eucalyptus grandis <400> 205 ccaccgcaga atccccccgg agttgaagcc accctgaact tttctcatat gcccgagccc gaacgtgaag ccagcccagg aactt cagtccctgg tgaatgccat tccagaacac aaaccttgct aatcatgttc ttgtctggtg gacaatctga gaaccaatcg ncaaacccgt ggcacgnatc ctgcttgaag acatggggag gaagacccga tgtccgcgcc aagggcaaat ctcttgctca 120 180 240 245 <210> 206 <211> 510 <212> DNA <213> Eucalyptus grandis <400> 206 tgagaagcga tcatccccag gccaccaccg gccgatgaac atggacgaat gaagccaacc atctccggtg atggttgatg tgctttggcg gagaagcaaa tccttgacaa tgcgctgcca tcgtcaagac caaatgccac gccaagccta ccatcctctt tacttgttga gtttaacaat g ca agaa g ca gtccgagtgg tcctgccacc cgcgaaaacc t tgcgggaag cagaactctg cgaggagact gcagaacatc gagtctggtg atggcctctg gtgaagggtc gcctctgtcc atcgcatcgc cgtcttgcct ctggtgacag ctctaccagt gtccccggta cttctgcaac agtctatccg tcactgtcaa caggacgtgg cgattggact ttccagggct ccacagtcga tcaaagtcga gctgctcaag ccacgcctcg agccagtaat aattttggcc agagaacacc cggcgagtac tggacgcaag caagggt tgg 120 180 240 300 360 420 480 510 <210> 207 <211> 413 <212> DNA <213> Eucalyptus grandis <400> 207 tggtgccttt ggctgggtcc cccgcacggc tgcttactac gcatccccaa tggcccatct acgctgccat ttgccaagac atggagagca cgggatcgag tctactacat ggctgagaac tcactcccgg cgctgagtgc aacgacgagt cagcagggtq gccctggccg aatggattga aggacattcg aatgtactgt aaagagaggg cc tggtgc ca cacgctttqc tgaaggaagc ccccaatagt aggtggccct tcgagggcat ccactcccca agggctcgat taaatqqaqa cgcctggggt ggagCcggag gaaggtgtgg cctcttgaag gcaagtggcc ggattagcat actqtqqtqa cttgcccgct atcttgctgg gcggaggtgt cccagcatgg gag <210> 208 <211> 434 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 208 gctgtccctg ccgttgtgtt ctcaatgcca tgaacaagct cgggctcttc agcagagcac gcacaggctg cattatttac aagggtgacg caaagcttgg tactaaggtg cttttgctgg gagatatect atttttgtac ttcattgatg ttgt tttgtctggt caagggcaag tttgaagtct taggtgcaag cgagggagct cggtattttc taggataata gggcagagtg aaaccatggt tgggntggaa gcaaactcag gctgaaagtc tgcttttcat agcatgactg aggaggaggc ctctttcctt aggaggagaa aggcaactct tccatgttaa ttgagaaaaa tactgtactg aaccctcaac ctcctttgga cgtgcccaag tggtacttac ggattacaag taggtgtctg gtaatgcatt 120 180 240 300 360 420 434 <210> 209 <211> 350 <212> DNA <213> Eucalyptus grandis <400> 209 acttgtagtc cttaacatgg tgtatgttcc aagagttgcg ccttgggaat gttctcttcc gaccaaaaga gaaggagagg ggttcaaagt ggcctcctcc caggaggcac tgttcgctgc agagactcgg tccgagttgg tttccagccc ctccatggct tcactctgcc agggcacgaa aagcaccctc ccttgcatct acgccttcaa tcttgccctt caccggacaa cggtgtactc gccaagttga gcatcaccct tctgagaagt gcagcctgtg ggtgctctgc tgtagggccc gagcttgttc atggcattga gaagacaatg gccggaacag agcaatgacc 120 180 240 300 350 <210> 210 <211> 455 <212> DNA <213> Eucalyptus grandis <400> 210 gcgaaaaaca atggcctcgg gtttgtgaag ggcacgcaga caccaccgcc ccctccgctc gactgcgaaa acagttgcat aacctgcggg aagcgtttgg ciacaggaca ctcctggtca cttcgaggag actctctacc tgagcagaac attgtccctg cttctctcct ctctccgcac tcgtcgtcaa cacccgggag cgtcgatcgg gtgctccggg aatccaccac gtattaaagt caagtcatct cccatctctc agccagtgcc aggaatcctg gctagagaac gcttggccag cgacggccgc cgaca cccgtgctcg gccgccgtcc tatgctgatg gccatggacg accgaggcca tacatctctg aagatggtcg acaagtcgga gctaccaccc agctcgtcaa agtcaaacgc accgccaggc gtgctatcct acgtcctcgt 120 180 240 300 360 420 455 <210> 211 <211> 509 <212> DNA <213> Eucalyptus grandis <400> 211 ggagggagaa gctttgagaa gagtttggcc tttgcaagcg gtgctcgaca agtcggagtt gccgtccgct accaccccac gctgatgagc tcgtcaagac atggacgagt caaacgcaac gaggccaacc gccaggccta atctctggtg ctatcctctt atggtcgacg tcctcgttga gagataaggt aaaaacaatg tgtgaagggc caccgccccc tgcgaaaaca ctgcgggaag caggacactc cgaagagact gcagaacat aacacagttt gcctcggctt acgcagactc tccgctctcg gttgcatcac cgtttggcgt ctggtcagtg ctctaccaat ttgcttgccg ctctcctcaa tccgcacccc tcgtcaaagc ccgggagagg cgatcgggct ctccggggct ccaccaccga ggctgtgctc qtcatctccc atctctcgcc cagtgcctat aatcctggcc agagaacacc tagccagtac cggccgcaag 120 180 240 300 360 420 480 509 <210> 212 <211> 364 <212> DNA WO 00/22092PCNZ9016 PCT/NZ99/00169 <213> Eucalyptus grandis <400> 212 atttgaagtt gctcagaagg catgtttgag ggtatcctcc caaggccact cctcaacaag cccggcagtc cctggaatca gaacttgaat gccatgaacc agccctccag aacacctgct ccag tttgggctga ttaagcccag tcgctgaata tgttcttgtC aatcgccaaa tgaagacatg ggttttctac catggtcact caccctcaag tggtggacaa cccgtggcac gggaggaaga tacctggctg cctggtgccg cttctccacc tctgaagttg gtatctttct cccgagaacg agaacaatgt agtgcaagga gcagaatccc aagccaccct catacgcccg tgaagccagc 120 180 240 300 360 364 <210> 213 <211> 372 <212> DNA <213> Eucalyptus grandis <400> 213 ctgaagttga agccaccctg tatctttctc atacgcccga ccgagaacgt gaagccagcc ctcagcttgg caagtacaca tcaagggcta cgtgtattaa gacttaagaa ttgatccctc catcgaatgt ac aacttgaatg gccctccaga caggaaacct.
ggtgagggcg gctgttcact tcagcgtgtt ccatgaacca acacctgctt tgcttgtccg agtccgagga gtaggtggaa atgattatag atcgccaaac gaagacatgg cgccaaggcc ggccaagaaa gtggatgatc ccacggagac ccgtggcacq ggaggaagac aactctcttg ggaatgttcq aaagt tggga tatttttgca 120 180 240 300 360 372 <210> 214 <211> 471 <212> DNA <213> Eucalyptus grandis <400> 214 atattaacct tcacaacatc taggatatct aaagcacctt tttgcgtcac aatgcagcct tgctgaagag ttcatggcat cctcatataa aatgaaaatg ccagacacct agtacttgta ccttgtaagt gtgccttggg cccgtccaaa tgaggttgag aaatataaaa cattaccagt atttttctta atccttaaca accaagagtt cacgttctcc ggagaaggaa ggttgcctcc cttcataaag acagtcatgc aaaggaaaag tggagacttt gcctctgagt tcctttccag agagaccatg tcctcactct aagtcgcaaa ttattatcct cagaaaatac cagcagctcc ttgccttgca cccaagactt gtttcttgcc gcccaccaga cattcaacca agtacaaaaa cgccagcaaa ctcgccaagc cctagtaaat caaagtgctc cttgagcttg
C
<210> 215 <211> 465 <212> DNA <213> Eucalyptus grandis <400> 215 acqgtcagta tccgcccctc tgagcatcac agtgcagcct tgctgtaggg ttcatggcat atggccggaa acctccggag atccacgaaa agtacttgta ccttgtatgt gtgccttggg cccgaccaaa tgaggttcaa cagcaggagg caaccttggg caaacgaagc gtccttaaca tccaagagtt aatgttctct agagaaggag a gt ggc c tcc cactgttcgc ggcatctgat acaagtaata tggagagact gcgt ccgagt tcctttccag aggctccatg tcctcactct tgcagggcac ccgggggtga gtatcgcagc cggaagcacc tggccttgca cccacgcctt gcttcttgcc gcccaccgga gaacggtgta ccatg aaagcctaga ctcgccaagt tcttctgaga caaggtgctc cttgagcttg caagaagaca ctcagcaatg <210> 216 <211> 484 WO 00/22092 WO 0022092PCTINZ99/00I 69 <212> DNA <213> Eucalyptus grandis <400> 216 ctcatataaa aatataaaac atgaaaatgc attaccagta cagacaccta tttttctcaa tacttgtaat ccttaacatg ttgtaagtac caagagttgc gccttgggca cgttctcctc cgtccaaagg agaaggaaag aggttgaggg ttgcctcctc gccg ttcataaaga cagtcatgct atgaaaagca gagactttca ctctgagttt ctttccagcc agaccatggt ctcactctgc agtcgcaaac tattatccta gaaaataccg gcagctccct gccttgcacc caagacttca ttcttgccct ccaccagaca attcaaccat gtacaaaaat ccagcaaaaa cgccaagctt tagtaaataa aagtgctctg tgagcttgtt aaaacacaac cacaacatca aggatatctc agcacct tag tgcgtcaccc tgcagcctgt ctgaagagcc catggcattg ggcagggaca <210> 217 <211> 362 <212> DNA <213> Eucalyptus grandis <400> 217 cgtccctcag tacttgtagt agcatcaccc ttgtatgttc tgcagcctgt gccttgggaa ctgtagggcc cgaccaaaag catggcattg aggttcaaag ggccggaaca gcaggaggca ct ccttaacatg caagagttgc tgttctcttc agaaggagag tggcctcctc ctgttcgctg gagagact cg gtccgagttg ctttccagcc gctccatggc ctcactctgc cagggcacga gaagcaccct gccttgcatc cacgccttca ttcttgccct ccaccggaca acggtgtact cgccaagttg ttctgagaag aggtgctctg tgagcttgtt agaagacaat cagcaatgac 120 180 240 300 360 362 <210> <211> <212> <213> 218 395
DNA
Eucalyptus grandis <400> 218 ccttaacatg gagagactcg caagagttgc gtccgagttg tgttctcttc ctttccagcc agaaggagag gctccatggc tggcctcctc ctcactctgc ctgttcgctq cagggcacga cattgatccg ggggtgacca gaagcaccct gccttgcatc cacgccttca ttcttgccct ccaccggaca acggtgtact tgttgggctt cgccaagttg ttctgagaag aggtgctctg tgagcttgtt agaagacaat cagcaatgac caaca agcatcaccc tgcagcctgt ctgtagggcc catggcattg ggccggaaca ct ccggag ca ttgtatgttc gccttgggaa cgaccaaaag aggttcaaag gcaggaggca accttggggg 120 180 240 300 360 395 <210> 219 <211> 416 <212> DNA <213> Eucalyptus grandis <400> 219 cgtccctcag tacttgtagt agcatcaccc ttgtatgttc tgcagcctgt gccttgggaa ctgtagggcc cgaccaaaag catggcattg aggttcaaag ggccggaaca gcaggag-gca ctccggagca accttggggg ccttaacatg caagagttgc tgttctcttc agaaggagag tggcctcctc ctgttcgctq catctgatcc gagagactcg gtccgagttg ctttccagcc gctccatggc ctcactctgc cagggcacga gggggtgacc gaagcaccct gccttgcatc cacgccttca ttcttgccct ccaccggaca acggtgtact atgttgggct cgccaagttg ttctgagaag aggtgctctg tgagcttgtt agaagacaat cagcaatgac tcaaca 120 180 240 300 360 416 <210> 220 WO 00/22092 WO 0022092PCT/NZ99/0I 69 <211> 452 <212> DNA <213> Eucalyptus grandis <400> 220 acggtcagta atccacgaaa tccgcccctc agtacttgta tgagcatcac ccttgtatgt agtgcagcct gtgccttggg tgctgtaggg cccgaccaaa ttcatggcat tgaggttcaa atggccggaa cagcaggagg acctccggag caaccttggg caaacgaagc gtccttaaca tccaagagtt aatgttctct agagaaggag agtggcctcc cactgttcgc ggc atct ga t acaagtaata tggagagact gcgtccgagt tcctttccag agg c tcc atg tcctcactct, tgcagggcac cc gtatcgcagc cggaagcacc tggccttgca cccacgcctt gcttcttgcc gcccaccgga gaacggtgta aaagcctaga ctcgccaagt tcttctgaga caaggtgctc cttgagcttg caagaagaca ctcagcaatg 120 180 240 300 360 420 452 <210> <211> <212> <213> 221 289
DNA
Eucalyptus grandis <400> 221 ctcagtactn gtagtcctta acatggagag cacccttgna tgttccaaga gttgcgtccg cctgtgcctt gggaatgttc tcttcctttc gggcccgacc aaaagagaag gagaggctcc cattgaggtt caaagnggcc tcctcctcac actcggaagc accctcgcca agttgagcat agttggcctt gcatcttctg agaagtgcag cagcccacgc cttcaaggtg ctctgctgta atggcttctt gcccttgagc ttgttcatgg tctgcccacc ggacaagaa <210> 222 <211> 460 <212> DNA <213> Eucalyptus grandis <400> 222 ggatcgccgg gcagcagtcc cccgccgcgt ctccgccccg ccaaatccat tgcatctcct gtgggaaaag gttagcatcc gacaacttct gttgaccacg aggagacact ttaccaatcg agaaaattgt tccaggcatt atgaatcctg gtgccaaggc ttcgccccgC atccgcgccg ggtcgtggta atcgggttgg cctggtctgg acaacagatg aaagt tgaca ttggatggat gcccccggtc ccgcttactc tccttgccat acaacactga gtgaatacat ggaagaaatt agggtttggt tggcttcaag gtccgccgcc cgacgagctc tgatgagtca ggtcaatcgt ctctggtgcc tgt tgactgc tcctcttcct cgcttccccg gtccagaccg aatgcaacat caagcttata attttgtttg ctgcgtgagg ggatccaata <210> <211> <212> <213> 223 373
DNA
Eucalyptus grandis <400> 223 cgtccctcag tacttgtagt agcatcaccc ttgtatgttc tqcaqicctqt cccttqqqaa ctgtagggcc cgaccaaaag catggcattg aggttcaaag ggccggaaca gcaggaggca ctccggacaa cct ccttaacacg caagagttgc tgttctcttc agaaggagag tggcctcctc ctgttcgctg gagagactcg gtccgagttg ctttccagcc gctccatggc ctcactctgc cagggcacga gaagcaccct gccttgcatc cacgccttca ttcttgccct ccaccggaca acggtgtact cgccaagttg ttctgagaag aggtgctctg tgagcttgtt agaagacaat cagcaatgac <210> 224 <211> 524 WO 00/22092 WO 0022092PCT/NZ99/00I 69 <212> DNA <213> Eucalyptus grandis <400> 224 ggatcgccgg cccgccgcgt ccaaatccat gtgggaaaag oacaacttct aggagacact agaaaattgt atgaatcctg aaggtgctcg gcagcagtcc ctccgccccg tgcatctcct gttagcatcc gttgaccacg ttaccaatcg tccaggcatt gtgccaaggc ttttgccaaa ttcgccccgc atccgcgccg ggtcgtggta atcgggttgg cctggtctgg acaacagatg aaagttgaca ttggatggat tggaggacag gcccccggtc ccgcttactc tccttgccat acaacactga gtgaatacat ggaagaaat t agggt ttggt tggcttcaag tggttagcat gtccgccgCC cgacgagctc tgatgagtca ggtcaatcgt ctctggtgcc tgttgactgc tcctCttcct gtccgctgaa tcct cgcttccccg gtccagaccg aatgcaacat caagcttata attttgtttg ctgcgtgagg ggatccaata tactacaagc 120 180 240 300 360 420 480 524 <210> <211> <212> <213> 225 332
DNA
Eucalyptus grandis <400> 225 acggtcagta atccacgaaa tccgcccctc agtacttgta tgagcatcac ccttgtatgt agtgcagcct gtgccttggg tgctgtaggg cccgaccaaa ttcatggcat tgaggttcaa caaacgaagc gtccttaaca tccaagagtt aatgttctct agagaaggag agtggcctcc acaagtaata tggagagact gcgtccgagt tcctttccag aggctccatg tc gtatcgcagc cggaagcacc tggccttgca cccacgcctt gcttcttgcc aaagcctaga ctcgccaagt tcttctgaga caaggtgctc ct tgagcttg 120 180 240 300 332 <210> 226 <211> 362 <212> DNA <213> Eucalyptus grandis <400> 226 cgtccctcag tacttgtagt agcatcaccc ttgtatgttc tgcagcctgt gccttgggaa ctgtagggcc cgaccaaaag catggcattg aggttcaaag ggccggaaca gcaggaggca ct ccttaacatg caagagttgc tgttctcttC agaaggagag tggcctcctc ctgttcgctg gagagactcg gtccgagttg ctttccagcc gctccatggc ctcactctgc cagggcacga gaagcaccct gccttgcatc cacgccttca ttcttgccct ccaccggaca acggtgtact cgccaagttg ttctgagaag aggtgctctg tgagcttgtt agaagacaat cagcaatgac <210> 227 <211> 506 <212> DNA <213> Eucalyptus grandis <400> 227 gtcactcccg ct caagc tcc gggcaatccg tggcacgtgt qgcaggcccg tcCCtcgCCC atgttcgtca tgatcacagg agtagaacag gcgctgagtg tccaccgcag aggtcgaagc ccttctccta agaacgtgaa agctcggcaa agggatacgt tttagttatg ctaattcctc caaagagagg gatcccgcct aaccctgaac cgctagagcc ggccgctcag gtacaccggt gtactaaggc ataatgatgg cttcct gccactcccc gccgttcccg ctgaacgcga ctccagaaca gatacgctcc gaaggcgagt gatgcactga tggcggatgg agcaagtcgc gaatcatgtt tgaaccagtc cctgcttgaa tggtccgtgc ctgaagaggc aactccatcra agcattggaa cgagtacacc Cttgtctggt cccqaaccca gacgtgggga caaggccaac caagaaggga gct cagaaga gctatgaaga WO 00/22092PCIZ/016 PCT/NZ99/00169 <210> <211> <212> <213> 228 283
DNA
Eucalyptus grandis <400> 228 ttgacaaggg tttggttcct cttcctggat atggattggc ttcaaggtcc gctgaatact ggacagtggt tagcattcct tgtggtccct gacttgcacg atatgctgcc atctctcagg agattcttct tgatggagac caccctattg ccaataacga acaagcaagg Ctgctcttgc at aa tgg C Ct agaggactct atcctggtgc caaggcttgg tgctcgtttt gccaaatgga agtaaaagaa gctgcgtggg tgtgcccatt gttgaacctq tga 120 180 240 283 <210> 229 <211> 450 <212> DNA <213> Eucalyptus grandis <400> 229 gtcactcccg gcgctgagtg ctcaagctcc tccaccgcag gggcaatccg aggtcgaagc tggcacgtgt ccttctccta ggcaggcccg agaacgtgaa tccctcgccc agctcggcaa atgttcgtca agggatacgt tgatcacagg gtttagttat caaagagagg gatcccgcct aaccctgaac cgctagagcc ggccgctcag gtacaccggt gtactaaggc gataatgatg gccactcccc gccgtrcccg ctgaacgcga ctccagaaca gatacgctcc gaaggcgagt gatgcactga agcaagtcgc gaatcatgtt tgaaccagtc cctgcttgaa tggtccgtgc ctgaggaggc aactccatga cgagtacacc cttgtctggt cccgaaccca gacgtgggga caaggccaac caagaaggga qct cagaaga 120 180 240 300 360 420 450 <210> 230 <211> 417 <212> DNA <213> Eucalyptus grandis <400> 230 gtcactcccg gcgctgagtg ctcaagctcc tccaccgcag gggcaatccg aggtcgaagc tggcacgtgt ccttctccta ggcaggcccg agaacgtgaa tccctcgccc agctcggcaa atgttcgtca agggatacgt caaagagagg gatcccgcct aaccctgaac cgctagagcc ggccgctcag gtacaccggt gtactaaggc gccactcccc gccgttcccg ctgaacgcga ctccagaaca gatacgctcc gaaggcgagt gatgcactga agcaagtcgc gaatcatgtt tgaaccagtc cctgcttgaa tggtccgtgc ctgaggaggc aactccatga cgagtacacc cttgtctggt cccgaaccca gacgtgggga caaggccaac caagaaggga gctcaga <210> <211> <212> <213> 231 663
DNA
Eucalyptus grandis <400> 231 aaatggcctg agcagtcctt ccgccccgat cat ctcctgg tagcatccat tgaccacgcc accaatcgac caggcattaa gccaaggctt ttgccaaatg cgccagcttc cgccccgcgc ccgcgccgcc tcgtggtatc cgggttggac tggtctgggt aacagatggg agi tgacaag ggatggattg gaggacagtg gccaagctca ccccggtcgt gcttactccq cttgccattg aatactgagg gaatacatct aagaaatttg ggtttggttc gcttcaaggt gttagcattc acgccacctc Ccccgcccg acgagctcgt atgagtcaaa tcaatcgtca ctggtgccat ttgactgcct ctcttcctgg ccgctgaata Cttgtggtcc ctcccagtgg cttccccgcc ccagaccgcc tgcaacatgt agcttataga tttgtttgag gcgtgaggag atccaataac ctacaagcaa ctctgctctt atcgccgggc cgccgcgtct aaatccattg gggaaaaggt caacttctgt gagacacttt aaaattgttc gaatcctggt ggtgctcgtt gcagtaaaag 120 180 240 300 360 420 480 540 600 WO 00/22092 WO 0022092PCT/NZ99/0OI 69 aagctgcgtg gggacttgca cgatatgctg gcatctctca ggataatggc cttgtgccca t tg <210> 232 <211> 435 <212> DNA <213> Eucalyptus grandis <400> 232 gcttattatc ctagtacaaa gcagaaaata ccgccagcaa aacagctccc tcgccaagct tgccttgcac ctagtaaata ccaagacttc aaagtgctct tttcttgccc ttgagcttgt cccaccagac aaaaacacaa aacagtgtac tcagc aataggatat aagcacctta ttgcgtcacc atgcagcctg gctgaagagc tcatggcatt cggcagggac ctccagacac gtacttgtaa cttgtaagta tgccttgggc ccgtccaaag gaggttgagg agccggaggc ctatttttct tccttaacat ccaagagttg acgttctcct gagaaggaaa gttgcctcct atagtacgtt caaatgaaaa ggagactttc cctctgagtt cctttccagc gagaccatgg tctcactctg gaaaggcacg 120 180 240 300 360 420 435 <210> <211> <212> <213> 233 352
DNA
Eucalyptus grandis <400> 233 acttgtagtc cttaacatgg tgtatgttcc aagagttgcg ccttgggaat gttctcttcc gaccaaaaga gaaggagagg ggttcaaagt ggcctcctcc caggaggcac tgttcgctgc agagactcgg tccgagttgg tttccagccc ctccatggct tcactctgcc agggcacgaa aagcaccctc ccttgcatct acgccttcaa tcttgccctt caccggacaa cggtgtactc gccaagttga tctgagaagt ggtgctctgC gagcttgttc gaagacaatg agcaatgacc gcatcaccct gcagcctgtg tgtagggccc atggcattga gccggaacag tc <210> 234 <211> 330 <212> DNA <213> Pinus radiata <400> 234 ctgagtacta atgggccttc tctctcagga attgcattga tggcacagaa gtgctgagca caaacagggt ggagttagct caatggtctt cagaagcctt caatgtgttg caaggagaga gaaagatttg gtgaaggaag gtgcccattg gaagtggcgg tttgagggta gcaacccccg ctaaatggcg aacagttgtc ctgcgtgggg acttgcacgt tggagccaga gattcttctg agaaagtctg ggctgaggtt ttttgttaaa gccaagtatg agcataccca tatgccgcta gatggagacc ttcttttact gtgactcctg <210> <211> <212> <213> 235 301
DNA
Pinus radiata <400> 235 actgnagcac aaggagagag caacccccga aaaggttgca gagtacactc taaaaatgct taagaggagg gtgccaccag ctgttcctgg ggttatgttc ttgtctggag gacagtctga ggttgaggca acattgaatt tgaatgcaat gaaccaaagc ccaaacccat ggcatgtttc cttttcatat gcacgtgcct tgcagaatac atctctcaag acctgaaagg gtcttccaga gactgttgaa gcagctcaga gggcgcttct tattcgggcc aaggataatt ctctggccca 9 120 180 240 300 301 WO 00/22092 WO 0022092PCT/NZ99/001 69 <210> <211> <212> <2 13> 236 368
DNA
Pinus radiata <400> 236 acaaacaggg tgcaagtttt cggagttagc tgtgaaggaa acaatggtct tgtgcccatt acagaagcct tgaagtggcg acaatgtgtt gtttgagggt acaaggagag agcaaccccc gggtgcca gctaaatggc gctgcgtggg gtggagcnag gagaaagtct attttgttaa gaaaaggt tg gaacagttgt gacttgcacg agattcttct gggctgaggt agccaagtat cagagtacac cagcattacc ttatgccgct ggatggagac tttcttttac ggtgactcct tctaaaaatg catgggcctt atctctcagg cattacattg ttggcacaga ggtgctgagc cttaagagga <210> 237 <211> 423 <212> DNA <213> Pinus radiata <400> 237 gacttgaaga acacggaggc cgggtctcgg acagtacatc gcaccgaagc aagaagattg agttgacaag ggtctggttc agacggcctc gcatcaaggt gcgtacagtt gtgagcattc gggtctcggc cgcaacgggc gat gaaccgacag tccggcgcca tagacatcct ctttggctgg gcgctgagta ccaacggccc aattgctcag ggcatacagg ttCtcttcga cgtgcaacag ttcaaacgac t tataagcaa ctctgCtctg gacaacggtc cagctgctcg ggaaactctg aacatagtcc gaatcttggt ggagctcgct gccgtgaaag tggttccata ttcagcgcgc taccagtcca ccggcatcaa gccaaggcct tcgccaaatg aagccgcatg gtggagcaga 120 180 240 300 360 420 423 <210> <211> <212> <213> 238 352
DNA
Pinus radiata <400> 238 cttgcattga cagaagcctt tggcacagaa caatgtgttg gtgctgagca caaggagaga ttaagaggag ggtgccacca aggttgaggc aactttgaat ccttttcata tgcacgtgcc gaagtggcgg tttgagggta gcaaeccctg gctgttcctg ttgaatgcaa ttgcagaata anaaagtctg ttttgttaaa aaaaggttgc gggttatgtt tgaaccaaag catcnctcaa ggctoaggtt gcccagtatg agagtacact cttgtctgga cccaaatcca gacctggaag ttcttttact gtgactcctg ctaaaaatgc ggacagtctg tggcatgttt gg 120 180 240 300 352 <210> 239 <211> 427 <212> DNA <213> Pinus radiata <400> 239 cagcgtccgg gccggggctt tcccgggaga ggcatcCtgg gtecatcy cttagaaca cgcgccgggt ctcggacagt gtccagcacc gaaggcaaga catcaaagtt gacaagggtc aggcctagac ggcctcgcat caaatgg acagcgaaga cgatggacga cggaggcgaa acatctccgg agattgtaga tggttccttt caaggtgcgc gctgatcaag gtccaacgcc ccgacaagca cgccattctc catcctcgtg ggctggttca tgagtattat acggcgaaaa acctgtggga t acaggcagc ttcgaggaaa caacagaaca aacgacgaat aagcaaggag cagtggcgtc aacggctggc tgctcgtcag ctctgtacca tagtccccgg cttggtgcca ctcgcttcgc 120 180 240 300 360 420 427 WO 00/22092 WO 0022092PCT/NZ99/00 169 <210> <211> <2 12 <213> 240 470
DNA
Pinus radiata <400> 240 gcacaaggag agagcaaccc gagggtgcca ccagctgttc ggcaacattg aatttgaatg atatgcacgt gccttgcaga tgaagcagct cagagggcgc gcgatactct gctgaaggtg cacatattaa gaatgtgggt gttaagcttt tatgtttatt ccgaaaaggt ctggggttat caatgaacca atacatctct ttcttattcg aaagtgagga catagttttc tanttggcac tgcagagtac gttcttgtct aagcccaaac caagacctgg ggccaaggct gtctaagaag ttacgggaag ttacaatcct actctaaaaa ggaggacagt ccatggcatg aagggtcttc aattctctgg ggaatgttcg aactcgttca gaacttttta tgcttaagag ctgaggttga tttccttttc cagagaatgt cccagcttgg ttaagggata atgcggatag 120 180 240 300 360 420 470 <210> <211> <212> <213> 241 396
DNA
Pinus radiata <400> 241 gattgctggt tccatccttt aaatttgttg actgtttgcg ttagttccac tgccaggatc tcaagatctg ctgagtacta agcataccca atgggccttc tatgccgcta tctctcagga gatggagacc attgcattga ttgcaacaga agagcagaat aaacaatgaa caaacagggt ggagttagct caatggtctt cagaagcttg ccctatacca attatgcccg tcttggtgcc gcaagatttg gtgaaggaag gtgcccattg aagtgg gtaccacaac gcatcaaagt agggtttgga ctaaatggcq ctgcgtgggg tggagccaga cgacgggagg tgacaagggt tggattagcc aacagttgtc acttgcacgt gattcttctg 120 180 240 300 360 396 <210> 242 <211> 273 <212> DNA <213> Pinus radiata <400> 242 aacaatgtgt tgtttgangg tattttgtta aagccaagta tggtgactcc tggtgctgag cacaaggaga gagcaacccc cgaaaaggtt gcagagtaca ctctaaaaat gcttaagagg agggtgccac cagctgttcc tggggttatg ttcttgtctg gaggacagtc tgaggttgag gcaacattga atttgaatgn aatgaaccaa agcccaaacc catggcatgt ttccttttca tatgcacgtg ccttgcagaa tacatctctc aag <210> 243 <211> 557 <212> DNA <213> Pinus radiata <400> 243 ggaagctgcg cattgtggag ggcggagaaa ccctgaaaag tcctggggtt tgcaatgaac gaatacatct gcttcttatt tgaaagtgag tggggacttg ccagagattc gtctgggctg t taaagccca gttgcagagt atgttcttgt caaagcccaa ctcaagacct cgggccaagg gagtcta cacgttatgc ttctggatgg aggttttctt gtL.ggtgac0 acactctaaa ctggaggaca atccatggca ggaagggtct ctaattctct tgctatctct agaccattgc ttacttggca tcctggtgct aatgcttaag gtctgaggtt tgtttccttt tccagagaat ggcccagctt caggacaatq attgacagaa cagaacaatg gagcacaag aggagggtgc gaggcaactt tcatatgcac gttgaagcag gggcgatact gtcttgtgcc gccttgaagt tgttgtttga aaaicaac caccagctgt tgaatttgaa gtgccttgca ctcagagggc ctgctgaagg 120 180 2 300 360 420 480 540 557 WO 00/22092PCIZ90I6 PCT/NZ99/00169 <210> 244 <211> 593 <212> DNA <213> Pinus radiata <400> 244 acgagggtct cggacagtac ccagcaccga aggcaagaag tcaaagttga caagggtctg gcctagacgg cctcgcatca aatggcgtac agttgtgagc catggggtct cgcccgctac agccagagat catgttggat aggtgtggtc cgaggtgttc tgctgaagcc aagcatggtt actgttgccc aatataccct atctccggcg attgtagaca gttcctttgg aggtgcgctg attcccaacg gcggcaattg ggagaacacg ttctacctag a ccc c tggtg gaaccttctc ccattctctt tcctcgtgca ctggttcaaa agtattataa gcccctctgc ctcaggacaa gcattgagag cagagaacaa cccgaatgta cgaagaagaa cgaggaaact acagaacata cgacgaatct gcaaggagct tctggccgtg cggtctggtt gactttcgaa cgtgctgttc aggagagggc ttccaccggc ctgtaccagt gtccccggta tg99tgc caag cgcttcgcca aaagaagccg cccatagtgg gtagcgctga gaaggcattc cagtcccgaa cgt 120 180 240 300 360 420 480 540 593 <210> <211> <212> <213> 245 485
DNA
Pinus radiata <400> 245 acgagggtct cggacagtac ccagcaccga aggcaagaag tcaaagttga caagggtctg gcctagacgg cctcgcatca aatggcgtac agttgtgagc catggggtct cgcccgctac agccagagat catgttggat aggtgtggtc cgaggtgttc tgctg atctccggcg attgtagaca gttcctttgg aggtgcgctg attcccaacg gcggcaattg ggagaacacg ttctacctag ccattctctt tcctcgtgca ctggttcaaa agtattataa gcccctctgc ctcaggacaa gcattgagag cagagaacaa cgaggaaact acagaacata cgacgaatct gcaaggagct tctggccgtg cggtctggt t gactttcgaa cgtgctgttc ctgtaccagt gtccccggta tggtgccaag cgcttcgcca aaagaagccg cccatagtgg gtagcgctga gaaggcattc <210> 246 <211> 477 <212> DNA <213> Pinus radiata <400> 246 caggatcaaa caatgaatct agtactacaa acagggtgca ggccttcgga gttagctgtc ctcaggacaa tggtcttgtg gcattgacag aagccttgaa cacaqaacaa tqtqttqttt ctgagcacaa ggagagagca agaggagggt gccaccagct tggtgccagg agatttgcta aaggaagctg cccattgtgg gtggcggaga qagggtattt acccctgaaa gtcctggggt gtttggatgg aatggcgaac cgtggggact agccagagat aagtctgggc tgttaaaqcc aggttgcaga tatgttcttg attagcctca agttgtcagc tgcacgttat tcttctggat tgaggttttc cagtatggtq gtacactcta tctggaggac agatctgctg atacccaatg gctgctatct ggagaccatt t t ttact t 9 actcctqgtg aaaatgctta agtctga <210> 247 <211> 337 <212> DNA <213> Pinus radiata <400> 247 gaacatagtc cccggcatca aagttgacaa gggtctggtt cctttggctg gttcaaacga cgaatcttgg tgccaaggcc tagacggcct cgcatcaagg tgcgctgagt attataagca WO 00/22092PC/Z90I6 PCT/NZ99/00169 aggagctcgc ttcgccaaat ggcgtacagt tgtgagcatt cccaacggcc cctctgctct ggccgtgaaa gaagccgcat ggggtctcgc ccgctacgcg gcaattgctc aggacaacgg tctggttccc atagtggagc cagagatcat gttggatgga gaacacggca ttgagaggac tttcgaagta gcgctgaagg tgtggtccga ggtgttc <210> 248 <211> 452 <212> DNA <213> Pinus radiata 180 240 300 337 <400> 248 gttttctttt acttggcaca atggtgactc ctggtgctga actctaaaaa tgcttaagag ggaggacagt ctgaggttga catggcatgt ttccttttca agggtcttcc agagaatgtt attctctggc ccagcttggg ggaatgttcg ttaagggata gaacaatgtq gcacaaggag gagggtgcca ggcaacattg tatgcacgtg gaagcagctc cgatactctg cacatattaa ttgtttgagg agagcaaccc ccagctgttc atttgaatgc ccttgcagaa agagggcgct cttgaaggtg ga gtattttgtt ccgaaaaggt ctggggttat aatgaaccaa tacatctctc tcttattcgg aaagtgagga aaagccaagt tgcagagtac gttcttgtct agcccaaacc aagacctgga gccaaggcta gtctaagaag <210> 249 <211> 358 <212> DNA <213> Pinus radiata <400> 249 cagaatacac agtaagggct ttctatctgg tgggcagagt tgcagaccaa gaagccctgg ctttgaagac atgggctgga ctcgatgcaa ggccaattct gcaatggtgt ttctgagagc cttcagagga gaggaggagg acattgncat aaggatgaga gatgccactt cttcatgtca ctgtgccacc ccaccttgaa tctcctttgg atattcctgc tgggcaagta agga ctat aa tgcagtgccc cttgaatgcc ccgggctctt ggctcaggct tgcaggtgat gtattgattg ggcataatgt atgaacaagc caggccagca gccttgttat tctgctaagg atgaccac <210> 250 <211> 341 <212> DNA <213> Pinus radiata <400> 250 aaaactatga cccacattct cctcactttc accttcagca gaataagaag cgccctctga gagatgtatt ctgcaaggca ggttcattgc attcaaattc taaccccagg aacagctggt taatatgtgt gagtatcgcc gctgcttcaa cgtgcatatg aatgttgcct ggcaccctcc atcccttaac caagctgggc cattctctgg aaaaggaaac caacctcaga tcttaagcat gaacattccc ttcttagact cagagaatta gccttggccc aagacccttc caggtcttga atgccatggg tttgggcttt ctgtcctcca gacaagaaca t <210> <211> <212> <213> 251 408
DNA
Pinus radiata <400> 251 gaaattIccag agaaagacct tgtgatctat gaaatgagtg ttcgatcctt cacagcagac aaatcaagtg ggttggaacc cagtatacgt ggaagctatc ttggtgttat tgaaaagatt cctcatcttc tagaacttgg cattaatgca gtggaattat taccagtgtt tgagtttgac gagtttgaat ttcaaaggca tccaaatcct cgtgaccata tgttaaatgt atggggctat tctacaatga acttcttttc tccaatgagc cggtatgctt ccactggtgg ggggccaita WO 00/22092 WO 0022092PCT/NZ99/00I 69 gcagcttcat tagaatttaa gaaaatggtc aaggccttgc atagtgcagg aattgaggtt attttggatg tggtttacaa ccatacaaat gaagcggatg atgagcat <210> 252 <211> 537 <212> DNA <213> Eucalyptus grandis <400> 252 ggcgaacacc accttttagg gttatccatc aatgttacga cagaatgggg ccccattctt cgtggtattc t ct ttgagaa ggaacgccgt agcacagtgt tgaaaccatt atacccagag caagtacatg ccacaccggt caaaaaggaa tcagatgcta tacaggaaca tcgcatgctc tatgctgact gtggaaattc caaaatggga ctgagtagct ccaactgatg ggtggaggat ctggaccatg aagatttcag ctgagctgac tcatgcgtgc aagtaggcat catggaaatt tgaaagctgc ctggtcacta ggaacagtca gtgagagaat tcaaggttgc agcaggatac atttagagac ggggccagca tccaggaatt agccgaggct taacaactgg atatggtgaa actctcatct aggaatttca tacaacacac aacttccagc ggcgaacttc ggagcttttc gctggcaaag ccggaggatg ttcttcttgt gccaaatcag ctggcactat gttatcg 120 180 240 300 360 420 480 537 <210> <211> <212> <213> 253 466
DNA
Eucalyptus grandis <400> 253 gtagcttgaa agctgcagcc ctgatgctgg tcactataac gaggatggaa cagtcaatat accatggtga gagaatactc tttcagtcaa ggttgcagga tgacagcagg atactacaac tggcacggca cggtgctata cccaagatgc gctctgcgca gaggctgctg aactggccag ggtgaattct tcatctgcca attcactggc acacgttatc ttcaacttca cctgagaagc gcaaagcaga aggatgcccc tcttgtcgtg aatcagtctt actatggaac gggatggtta cttgcatcga tggtgaagca atggggccac attcttcaaa gtattctcag tgagaataca gcgttcgcat ccttcccatt aatgcgtgac agtagc accggtccaa aaggaaggtg atg ct actgg ggaacaaaga gctcctgagc gcccagatgt cacgagcaac <210> <211> <212> <2 13> 254 364
DNA
Eucalyptus grandis <400> 254 agatggcgaa gaagcatggg gaaacgttgg tgactcttgc aagatccaga tcttgcatat ttggctgtga caccctcccg tcatgcgtgc atttagagac aagtaggcat ggggccagca catg ttgaaagtgc tccatccctc acagaccagt gttctcaaag aacttccagc ggcgaacttc aggctgtgat taccaaagtg ggggtaggag ggcgaacacc acc tt t tagg gttatccatc gtcgtttcac ggctgtggaa aaactacgag tgtacagtgt tgaaaccatt ataccccgiag cagtgcggtg gaagttgata tacatatcgc tatgctgact gtggaaattc caaaatooqca <210> 255 <211> 379 <212> DNA <213> Eucalyptus grandis <400> 255 ccagcatata cagaccagtg gggtaggaga aactacgagt acatatcgct tggctgggac accctcccgg ttctcaaagg gcgaacacct gtacagtgtt atgctgactt catgcgtgca tttagagaca acttccagca ccttttaggt gaaaccattg tggaaattca agtaggcatg WO 00/22092PC/Z9O16 PCT/NZ99/00169 gggccagcag gcgaacttcg ttatccatca taccccgagc aaaatgggac atggaaattt ccaggaattg gagcttttca atgttacgac aagtacatgc tgagtagctt gaaagCtcqca tccgaggctg ctggcaaagc agaatggggc cacaccggtc caactgatgc tggtcactat aacaactggc cagaggatg <210> <211> <212> <213> 256 370
DNA
Eucalyptus grandis <400> 256 gaagctttcg tgcagagttc ttggattggg tccttgtggg gatatcctgg gataggtgaa aggcagcaga ggcaaggggt ataattcaca gccacaagaa ttggaaggtt cttccttaag ttgccttggc aatgactact gagttacggt tttcagtgct caggcttttt actggtttct tggtactctc ttgaggatgg acccatcaaa acgatcagta gggct agagg tctgtgatgg aggtgttgat tataatatca ccctgtaaaa tctactgaag tccagataat aggagattac tgatcatggt atgattggga aatggttgga aatctcagaa gcaggttctt gatggctatt gatagagtac 120 180 240 300 360 370 <210> <211> <212> <213> 257 287
DNA
Pinus radiata <400> 257 ggaaatctta acaaggacat ctactacaga ctatctgctg gagtggatca aatacctata gaagatttca tggtcagctt catagacaaa gaaattacta ttggccttgg tccgtcaggt gatgggagat ggaagttccc tggtattgga gatcggcatg ttatatggac tttcaatcac gaactaaggt gaattccagt gatattctag tgatgagtat gtacagctgt tgaatgctat tcatgggaaa tccaattcaa accctgccca tcctttttct gctatga 120 180 240 287 <210> 258 <211> 396 <212> DNA <213> Pinus radiata <400> 258 gcggtatcca tcttatcctg tcagtgctat gataaggtat tattttagtc atatagttgc tatgatctct aaccttagat gggtccaagt gatgcaggtc agatggtggc tggaacagtt tcttgatcat ggagagagaa agagtaacgg ggatactagc cctttttatt ccacgtccga attacaataa cttatggtga tcctaggagc tacatggaaa catttttttt tactagtttg agctgctgga ctggcctgag gttttttctt agctga ttcccaggga tatgaatttc gatttccttg aagcaggagt gacagcccgt gagtggtat t ttggagcatt cctccagctg tattgcagta ggggtaatgg ttttccgcag ctcgtatgct 120 180 240 300 360 396 <210> <211> <212> <213> 259 420
DNA
Pinus radiata <400> 259 gagggtctca tgcagcagaa atgattcaat cgctgcagtc agatgtttgg tagtgaacag agatgagaga agttgcaagg gctttgataa ggaggctttc tgagagcctt tacgtatttc cttactgctg tttcocaagac ccagagatat cgaggaaata tctcaaattg cgaatgaggg ggtactataa atggcgcagc gctgctgcag tacctttaac tgagaaatgc aggcactgtt cacttcctat tttaaatatt tccggagggt aggtgaaaat ttacaatcgt caggactgat agagatgggt ccttgtatgg ctcattaggc gcaattgaac cct caggatg aattggaaat 120 180 240 300 360 WO 00/22092 WO 0022092PCTINZ99/001 69 cattcgtgaa ctttgttaag cagatgtaca ataagtctca agatggaggc tgcaatggta <210> <211> <212> <213> 260 378
DNA
Pinus radiata <400> 260 gttctgaaat ccaggatgcc gatacaatat ccgcttctaa gccttaaagg ctcttggtgt agagatgctc caacaaaata gcagcaggcc tgaaagttca agcttgtcta tacctcttcc ttcttcacag accgatcg aatgcaaagg cactttaaac ggatggagtg tgactggtct ggcttcaata atcttgggtt aacttcccct cattgcaagg gttatgcaag gcatatttgg tgctttaatg cttacagtgg tttatgtaat caattcaagc tattttgggg ctttggtgaa gttgtaaatc gcaatagtga tcttcctccg aggcttactt cattgtgga9 aatggtccaa tagtcaagaa tccagacatc 120 180 240 300 360 378 <210> 261 <211> 303 <212> DNA <213> Pinus radiata <400> 261 gtgataatga tctggcctac acggatcagt ggggcaggcg aaactatgag tatatttcgt tgggatgtga taatctccca gttttgaagg gaagaacgcc tgttcaatgc tatgctgatt acatgaggag tttcaaggag aattttgggg atcttctggg agaaaccatt gtggaaattc aagtgggaat gggtcctgca ggagagctga ggtatccatc ttatcctgag agtaatggta cctggagatt cccaggaatt ggagcatttc agtgctatga taagtatatg gtctctaacc tta <210> 262 <211> 385 <212> DNA <213> Pinus radiata 120 180 240 300 303 <400> 262 cgaaagagat ggatacagac ttttacatgc attgaaatgc ggctcttgtg cgtcaggtat aaatgcttta cctagatttg cgagggaata aacgaaacag gctatctcaa gaactcatgc aatgcatgtg agtttggatt ccatagccag aggataatga taaatgctgg atgaagaagc gaccgcccaa aagaacatag atcat aatgetggca acaaccgtcc atggaaagaa atatgatcag gaagcgaata ctggaaagaa agacatcggg gaagccagtt ggtattgagg attgtaaggc tctgctttta ttcaacaaat cggtat tgaa gtoggccgga tttcctgtga aatccagacc cctatctaag tcctgagaag <210> <211> <212> <213 263 330
DNA
Pinus radiata <400> 263 ggcaagatca cctccgctca tgccagtggg gtgacggctc ggctactctg ccggctccac aacctggact tcaagatcaa ggagccagca acttctacaa agctcctcct ctggcaacgc gtactatgtc cgagcccatc c tgg tt gt cc gattatcggt caaggccggc ctacttcgtc aacaacaagg ggtaactggg atgttcaaga gacgacctga ctgatcacct gtgttcccgc cccccatgaa acgagcctac gcgacaactg ctggcaacg cgagaagggc cctgggtgtt cacctctgcc cagctacgac ctgcactgtc <210> 264 WO 00/22092 WO 0022092PCTINZ99/00 169 <211> 359 <212> DNA <213> Eucalyptus grandis <400> 264 aatttggaca tcctgaatg aatgccggcg aagatttgat aatttgatca ggctatgcag aatacatatc cagaaaagat tgtttgtctt caatttccat agcctggaaa gntaagattg ttggactttc ctaggagatg catgttgaag gaaggagaca tggaataata tcttaaattc caagggaagg ctgagtatct aagcttatgg gggtcatcgt gctacacgga agatgacgcc gaacggtttc aagataccgg cttcatgact ctttgaaagg ctaccatgta ttgtttggag agttatgata ggaatgcaag tctgagcacc ggaaatcttg ggctgcttga ggtatagta <210> 265 <211> 451 <212> DNA <213> Pinus radiata <400> 265 atgcattggg tcactctgat aagccaatgc tgtgcctatc gagttgagct tgaagatcgg atoaaataga tccattgttg ataagaaaac aagagagttg gttatgaaaa gatgggattt ctggtgctaa gggggcatca ttatgctaag aacgagtttg gatggattgc caggccatcg ccaaaagttg aataactatc attgataaat aatagaagtg cttataggag gagtatggga gcaatactga aagaacaaac tccctccacc gtgaacatct atgaaggtgg cggctggaat atttcaacaa g ggacgcgtca tactaagcca tgggagtggc tgattatcga cttggaagca cacatacaga ctggaatccc catgactctg atgcaaaaca caaaggat tt tttgcgcagt ttttctaggg gagtgggcac aatgctgatg 120 180 240 300 360 420 451 <210> 266 <211> 375 <212> DNA <213> Pinus radiata <400> 266 gagatttcaa caactggaat gggagatatt tttaccaaac ttaagataca tatggataca ttgctgttca agctccgggt aggataaata tgaatttaag aagcgcatgt tggcatgagc atgatgtact accac cccaatgctg aatgcagatq atttcgggac gag-attccat tatcctcgac agcacggaac atgttatgac gttctccacc caaaggacgc acaatggaat caaagcagcc ccaaaattaa taagaacgag aattccccat aatccctgct atattatgat caaatcattg tacatatgtt tt tggagtat ggatctcgtg tggattaagt ccccctcctg cgaatatatg gagttcaggg 120 180 240 300 360 375 <210> 267 <211> 408 <212> DNA <213> Pinus radiata <400> 267 actctgccat gtcatgtttg acaagatgat tcatttcata gaaatgagtt tggtcatccg atgaaaaatg tagaagacag tgaataattt tgacaaagcc caaagcaaat agttagcatt ataiagtgtt tgttttcaac caatcacctt a ca at ggca c gaatggattg tggaacttag atgaatgagc gcaaatgaag ttccatccca ctccagttgt taggtggcga actttccaag tggacacaga ttgatgagaa aagataaggt agaatacata tgatcgtggg gggctatctt ggaaggaaat tcacctgaga atttcatttc gattgttttt tcctgggt attgcacttc aattttatgg ggttggagct tacaagttta ctcgcatcgt gaacgaggcg <210> 268 <211> 476 WO 00/22092PC/Z9016 PCT/NZ99/00169 <212> DNA <213> Pinus radiata <400> 268 ccgagcctcg agtgggcacc agttgggata taatgttatc ttggttatca ggtcaccagc tcaaggagct gatcgatacc actctcacgc ctgcaagaac agtacttcca tggaggacca actctcatta tgaggttctc atcagtttga cggtttccgc tacaaagagt cagctgatgg tttttcgctg gcgcattcaa gtgctcgatg aagggatacc cgcttcctga tttgatggtq ttactttaaa cagttatgga ccagctctcg tgggcatcac gcttgaacca acgacttgtg tgtctaatct ttacgagtat cactcttcct acatgcctac atatggtact tgtattgctt gttcgatggg ggacagtcgt gcgcttctgg gttgtatctg cgcattaaga tatgcttcct cctgaggagc gatgtagtac actgaccacc ctcttcaact atggaagagt catcac 120 180 240 300 360 420 476 <210> 269 <211> 313 <212> DNA <213> Pinus radiata <400> 269 gaacagttca cccgaggata cgagaagttt gtctaccacg aatgggcccc gaacgccgta tgggaccgac actctcatgc tatgaccaaa cctagcatca acggacagcc tgctatcccc attcctggcg gcgagcgtat cgagcgtctg ctctctgtct cgc ggactcaacg gaggcctccc gatcagtatg cacgattcga cctgcttgga ccaagcccga taatcggaga gtgtctgggg aaataaaggt tcaagcgcgt tggctctatc tttcaacaac aatcacgatc ttcgttcgtt cacccaggac <210> 270 <211> 258 <212> DNA <213> Pinus radiata <400> 270 aacatctatg atgtacaccc atcacggatt acaggtagca ttcactggga attatgctga gtactttgga tttgctactg atgtagatgc tgtggtttat ttgatgctgg ttaatgacat gattcatggt Ctatttcccc aggccataac aattggagaa gatgtgagtg gtatgccaac cttttctcgt tctgtacaag atggcggagt gggatttgat tatcgactcc atatggcagt agctgacaaa tggattga <210> 271 <211> 349 <212> DNA <213> Pinus radiata 120 180 240 258 <400> 271 cctatccagg Ccttegaaga gatcqgccaa aaqttqtccc ttgttgaata actatcgtga gagttgattg ataaatatga ggatttaata gaagtgcggc gcatcactta taggagattt acaaactact tccacctgg acatcttgat aggtggcttg tggaatcaca caacaactgg aagccaatgc aqtgqccaaa tatcgatttg gaagcatttt tacagagagt aatccaatgc aaaacagagt gga tt ta tqa cgcagtataa ctaggggtta gggcacctg tgatgttat ngagcttgaa aatagatcca gaaaacaaga tgaaaagatg tgctaagggg 120 180 240 300 349 <210> 272 <211> 369 <212> DNA <213> Pinus radiara <400> 272 WO 00/22092 WO 0022092PCT/NZ99/001 69 agcctggaaa gaataaatca cctttcttgt tgagaataaa cgcaacatgt gcttctccca tgtagtcag gtacaaggtg caatgcaagg gtatgcacct aataagatgt ttgtatgact ttatcttgtt gtactggatt tttcacacta tgcagaactt ttgccttgta gaagaaagca tttccatata ctgatgaaac ctgagggatq cagttgtcta tccaaatttt gtatttctaa aatctcactt aaaatttggc qtatgatgat tagtcttaca accggaaggg aacagatatc gggcatacca ggttttgcca cggccccact gatgattaaa aatacatgat ggaggacatg tctttagttc 120 180 240 300 360 369 <210> 273 <211> 327 <212> DNA <213> Eucalyptus grandis <400> 273 ctcctttctc tctctacaca accctccacc tccacccgcg agggcctcat cgtgagcttc tctccctggc ggaggccccg cgatcgccgt gacccgcctc gagttcgggc aacaatgctg tacgctgttt atcccgatgg ggcgagatgc gggt tcc tc a ggcggccggt gccggat ctctctcttc tcgcctcctt cctccttcga cttcccgcaa caatggcatc tccggcggca tcatcgactt cgtcccgacc gtgtcggggg aggcccccgg cggcgccccc gccaacgtcg ccgcgttcgt cggcaaagct cggggacgac <210> 274 <211> 275 <212> DNA <213> Eucalyptus grandis <400> 274 acattttaaa gcaactatgt tgacattctq gcattagctt ttgttaccaa tagagctgat ccaagtgcgg ccatgctttt acatgaatcg aaagatcttc cattatggtt ctatcagttt tgcagcaatg aaaattgcca aaaactcagg gtgtgcggta tgctcacaat gacaagaact ggggagcgag agttcctatt ttttcgtcat gaaaciagat gttgaactta tcagcaaggc gattgatgaa ccctgcaaat cggctcatct gagcg <210> 275 <211> 362 <212> DNA <213> Eucalyptus grandis <400> 275 gcaacaatgg catctccggc acttcgtccc gaccgtgtcg cccggcggcg Cccccgcaac tcgtcggcaa gatcggggac acggggtcaa ctgcgacggc taacgctccg tgccgacggg to ggcaagggcc ggggtctccc gtcgcgatcg gacgagttcg atcaacttcg gagcgcgagt tcatcgtgag tggcggaggc ccgtgacccg ggcacatgct accagggggc tcatgttcta cttcggcgag cccggggttc cctcggcggc ggccgggatc gcggaccgcq ccggaacccg atgctcatcg ctccaacgcc cggtccgagt ctaaacgaca ctggccttcg agcgccgaca <210> 276 <211> 543 <212> DNA <213> Eucalyptus grandis <400> 276 gcttagtgtc cccacaagcc caaaaatttt attaggatta tttatatatc ttaagaaagc aaaggagtct gtgacaccat acgcagggga gcaatgggaa tacagaagca gcaggaatgc taaccagact ctcttcaatt gaaaatggaa gcaagcaacg actagtccta agggaaaatc agagaatgga agaattggag Cttgttcgaa tgctttattt cgtgccatcg atcaagctga 120 180 240 WO 00/22092 WO 0022092PCTNZ99OO 169 ggacatcagc ctcggtcggg agggcgggga cgcacgcgtt tgcaaacttc aggactttcc ggtcgtcaan aatgttgcag aggagtgccc caaccgtgtt aacatggaat gcttcnacat aaccgtgctc acccaaagta acaaggagaa cat tcgctccctt tcaattttcc cgacaaagga gtccatgaaa gtgtcaagtt Cttggtggtg ttcatcttcg atcaccagcc atgcttggtg ggggtgccac gtgatggctc agaatggagc ccggttgtgt tagtatctac agtgtcattg 300 360 420 480 540 543 <210> 277 <211> 163 <212> DNA <213> Eucalyptus grandis <400> 277 agcntttggg acaaggcaga tatcattaaa gggagtgaca agattgatga tgaaaatgca ctccttgtta ctttgggtga gcacggttgt gtgagtgatq ttgaactgga gttcctcaca atgacactgt ggcaccccaa cttgacactt agatactaca cca 120 163 <210> 278 <211> 270 <212> DNA <213> Eucalyptus grandis <400> 278 cacggttgac acaaccgggg ctggtgattc tgacgaccgc tccattctcg aagatgaagg cgcgtgcgga ncatcaccac caccaagaag gatgtcctca gcttgatcga tggcacgaaa attctctgat tttcccttag gactagtcgt ctttgtcggg gcactcctct gcaacattgt aaaattgagg aaagtcctga agtttgcaaa ggagcgatcc ccgccctccc gaccgaggct taaagcattc gaacaagctc caattcttcc 120 180 240 270 <210> 279 <211> 201 <212> DNA <213> Eucalyptus grandis <400> 279 cacggttgca cacaaccggg gctggtgatt cctttgtcgg ggcactcctc tgcaacattg ttgacgaccg ctccattctc gaagatgaag gaaaattgag gaaagtcctg aagtttgcaa acgcgtgcgg agccatcacc accaccaaga agggagcgat ccccgccctc ccgaccgagg ctgatgtcct cagcttgatt g <210> 280 <211> 319 <212> DNA <213> Eucalyptus grandis <400> 280 gcgccgacgg ggagcgcgag ttcatgttct accggaaccc gagcgccgac atgntgctca agcccgagga gctcaacctc gagctgatca gatctgcgaa agtctttcat tatggatcca tcagtttgat tgtggagcca tgcagatccg cccatcttga agcaatgcaa gttgccaagg acgctggggc tctgctctcc tatgatccaa acctcagact accattgtgg ccatcacctg aggaggctcg tgagcagatc aagagcattt ggggacaaag gcagatatca tttaaaagtg gagtgattgt tgaactgga <210> 281 <211> 446 <212> DNA <213> Eucalyptus grandis 120 180 201 120 180 240 300 319 WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 281 gcgatcccga tggcttcccg ttcggcgaga tgctcattga ccggggttcc tcaaggcccc ctcggcggcc ggtccgCgtt gccgggatcc tgaaggagaa cgtgaccgcg ctggccttcg ccggaacccg agcgccgaca gatctgcgaa agtctttcat caacaatggc cttcgtcccg cggcggcgcc cgtcggcaag cggggtcaac tcacgctccg tgctgctcaa tatgga atctccggcq accgtgtcg cccgccaacq ctcggggacg tgcgacggca cgccgacggg gcccgaggag gcaagggcct gggtctccct tcgcgatcgc acgagttcgg tcaacttcga gagcgcgagt ctcaacctcg catcgtgagc ggcggaggcc cgtgacccgc gcacatgctg ccagggggcg tcatgttcta agctgattan 120 180 240 300 360 420 446 <210> <211> <212> <213> 282 369
DNA
Eucalyptus grandis <400> 282 tccaaagaat tcagcacgag ctccgcgccg acggggagcg ctcaagcccg aggagctcaa atccatcagt ttgattgtgg caaggacgct ggggctctgc acctgaggag gctcgtgagc gagtgatgt cttcgaccag cgagttcatg cctcggggct agccatgcag tctcctatga agatcaagag ggggcgcgga ttctaccgga gatcagatct atccgcccat tttaaacctc catttgggac ccgcgctggc acccgagcgc gcgaaagtct cttgaagcaa agactaccat aaggcagata cttcgtcacg cgacatgctg t tcat tatgg tgcaagttgc tgtggccatc tcattaaagt 120 180 240 300 360 369 <210> 283 <211> 583 <212> DNA <213> Eucalyptus grandis <400> 283 ccgcgatccc gatggctttc gcttcggcga gatgctcatt ccccggggtt cctcaaggcc ggcttggcgg ccggtccgcg tggccgggat cctgaaggag cgcggaccgc gctggccttc accggaaccc gagcgccgac gatctgcgaa agtctttcat ccatcttgaa gcaatgcaag cctcagacta ccattgtggg cgcaacaatg gacttcgtcc cccggcggcg ttcgtcggca aacggggtca gtcacgctcc atgctgctca tatggatcca ttgccaagga catcacctga gcatctccgg cgaccgtgtc cccccgccaa tgctcgggga actgcgacgg gcgccgacgg agcccgagga tcagtttgat cgctggggct ggaggcttcg cggcaagggc gggggtctcc cgtcgcgatc cgacgagttc catcaacttc ggagcgcgag gctcaacctc tgtggagcca ctgctctcct tga ctcatcgtga ctggcggagg gccgtgaccc gggcacatgc gaccaggggg ttcatgttct gagctgatca tgcagatccg atgatccaaa <210> 284 <211> 305 <212> DNA <213> Eucalyptus orandis <400> 284 ctccttcctc cttcgaaccc tccggcggca agggcctcat gtgtcggggg tctccctggc gccaacgtcg cgatcgccgt ggggacgacg agttcgggca cgacg tncacccgcq cgtgagcttc ggaggcccc9 gacccgcctc cat gc tggcc atcccgatgg cttcccgcaa caatggcatc qgcgagatgc tcattgactt cgtcccgacc gggttcctca aggcccccgg cggcgccccc ggcggccggt ccgcgttcgt cggcaagctc gggatcctga aaggagaacg gggtcaactg 120 180 240 300 305 <210> 285 <211> 403 WO 00/22092 WO 0022092PCT/NZ99/001 69 <212> DNA <213> Eucalyptus grandis <400> 285 tctacacata cgctgtttct ttcgaaccct ccacccgcga gggcctcatc gtgagcttcg ctccctggcg gaggccccgg gatcgccgtg acccgcctcg gttcgggcac atgctggccg cttcgaccag ggggcgcgga ctctcttctc tcccgatggc gcgagatgct ggttcctcaa gcggccggtc ggatcctgaa ccgcgctggc gcctcctctg ttcccgcaac cattgacttc ggcccccggc cgcgttcgtc ggagaacggg cttcgtcacg caaatccatc aatggcatct gtcccgaccg ggcgcccccg ggcaagctcg gtcaactgcg ctc tccttcctcC ccggcggcaa tgtcgggggt ccaacgtcgc gggacgacga acggcatcaa <210> <211> <212> <213> 286 471
DNA
Eucalyptus grandis <400> 286 gttcctcaag gcccccggcg cggccggtcc gcgttcgtcg gatcctgaag gagaacgggg cgcgctggcc ttcgtcacgc cccgagcgcc gacatgctgc gaaagtcttt cattatggat tgaagcaatg caagttgcca actaccattg tggccatcac gcgcccccgc gcaagctcgg tcaactgcga tccgcgccga tcaagcccga ccatcagttt aggacgctgg ctgaggaggt caacgtcgcg ggacgacgag cggcatcaac cggggagcgc ggagctcaac gattgtggag ggctctgctc tcgtgagcag atcgccgtga ttcgggcaca ttcgaccagg gagttcatgt ctcgagctga ccatgcagat tcctatgatc atcaagagca cccgcctcgg tgctggccgg gggcgcggac tctaccggaa tcagatctgc ccgcccatct caaacctcag t 120 180 240 300 360 420 471 <210> <211> <212> <213> 287 410
DNA
Eucalyptus grandis <400> 287 tctctctcta cacatacgct cacctccacc ggcgatcccg tcatcgtgag cttcggcgag tggcggaggc cccggggttc ccgtgacccg cctcggcggc ggcacatgct ggccgggatc accagggggc gcggaccgcg gtttctctct atggcttccc atgctcatcg ctcaaggccc cggtccgcgt ctgaaggaga ctggccttcg ctcctcgcct gcaacaatgg acttcgtccc ccggcggcgc tcgtcggcaa acggggtcaa tcacgctccg ccttcctcct catctccggc gaccgtgtcg ccccgccaac qctcggggac ctgcgacggc cgccgacggg tcgaaccctc ggcaagggcc ggggtctcc gtcgcgatcq gacgagttcg atcaacttcg <210> <211> <212> <213> 288 451
DNA
Eucalyptus grandis <400> 288 cgagttcggg cacatgctgg caacttcgac cagggggcgc qcgcqagttc atgttctacc caacctcgag ctgatcagat ggagccatgc agatccgccc ctctcctatg atccaaacct cagatcaaga gcatttggga ttcctcacag ggagtgacaa ccgggatcct ggaccgcgct ggaacccgag ctgcgaaagt atcttgaagc cagactacca caaggcagat gattgatgat gaaggagaac ggccttcgtc cgccgacatg ctttcattat aatgcaagtt ttgtggccat atcattaaag ggggtcaact acgctccgcg ctgctcaagc ggatccatca gcaaggacgc cacctgagga tgagtgatgt gcgacggcat ccgacgggga ccgaggagct gtttgattgt tggggctctg ggctcgtgag tgaactggag 120 180 240 300 360 420 451 <210> 289 WO 00/22092 WO 0022092PCT/NZ99/001 69 <211> 361 <212> DNA <213> Eucalyptus grandis <400> 289 ccatgttaac acggttgaca caacattgtt gacgaccgct gtttgcaaac gcgtgcggag gaccgaggct gatgtcctca caattcttcc attctctgat tgaagagagt ctggttagca caaccggggc ccattctcga ccatcaccac gcttgatcga tttcccctag ttcctgctgc tggtgattcc agatgaagga caccaagaag tggcacgaaa gactagtcgt ttctgtattc tt tgt cgggg aaattgagga ggagcgat cc taaagcattc tgcttgcttc ccattgctcc cactcctctg aagtcctgaa ctgccctccc gaacaagctc cattttcaat cctgcgtatg 120 180 240 300 360 361 <210> <211> <2 12> <213> 290 347
DNA
Eucalyptus grandis <400> 290 gcggcaaggg cctcatcgcg cggtggtctg cctggcggag acgtggcgat cgccgagacc acgacgagtt canccacatg gcatcaacta cnaccagagg ggaagcgcta gttcntgttc agctcnagcg gccccggggt cggctggggg ctggccggga gcgcggaccg taccggaacc agatgctgat tcctcacggc gccggtccac tcctgaagga agctgacctt cgagcgccta tgactntgac ccccggcggg gttcgtcggg gaacggggtc caacacgctc catgctg ccgaccgtgt gcccccgcca aagctcgggg aactgcgacg cacgcccact 120 180 240 300 347 <210> 291 <211> 335 <212> DNA <213> Eucalyptus grandis <400> 291 ccacccgcga tcccgatggc gtgagcttcg gcgagatgct gaggccccgg ggttcctcaa acccgcctcg gcggccggtc atgctggccg ggatcctgaa ggggcgcgga ccgcgctggc ttcccgcaac cattgacttc ggcccccggc cgcgttcgtc ggagaacggg cttcgtcacg aatggcatct gtcccgaccg ggcgcccccg ggcaagctcg gtcaactgcg ctccg ccggcggcaa tgtcgggggt ccaacgtcgc gggacgacga acggcatcaa gggcctcatc ctccctggcg gatcgccgtg gttcgggcac cttcgaccag 120 180 240 300 335 <210> <211> <212> <213> 292 643
DNA
Eucalyptus grandis <400> 292 ccccggcggc gttcgtcggc gaacggggtc cgtcacgctc catgctgctc ttatggatcc agttgccaag gccatcacct taaagtgagt tgcaatgaca tgtagatact gcccccgcca.
aagctcgggg aactgcgacg cgcgccgacg aagcccgagg atcagtttga gacgctgggg gaggaggctc gatgttgaac ctgtggcacc acaccaagca acggcgcgat acgacgagtt gcatcaactt gggagcgcga agctcaacct ttgtggagcc ctctgctctc gtgagcaga t tggagttcct ccaacttgac ttttcatgga cgccgtgacc cgggcacatq cgaccagggg gttcatgttc cgagctgatc atgcagatcc ctatgatcca caagagcatt cacagggagt acttctnctt catgtggaag cgcctcggcg ctggccggga gcgcggaccg taccggaacc agatctgcga gcccatcttg aacctcagac tgggacaagg gacaagattg gttactttgg cat gccggtccgc tcctgaagga cgctggcctt cgagcgccga aagtctttca aagcaatgca taccattgtg cagatatcat atgatgaaaa gtgaacacgg 120 180 240 300 360 420 480 540 600 643 WO 00/22092PCNZ90I6 PCT/NZ99/00169 <210> <211> <212> <213> 293 300
DNA
Eucalyptus grandis <400> 293 ccacccgcga tcccgatggc ttcccgcaac gtgagcttcg gcgagatgct cattgacttc gaggccccgg ggttcctcaa ggcccccggc acccgcctcg gcggccggtc cgcgttcgtc atgctggccg ggatcctgaa ggagaacggg aatggcatct gtcccgaccg ggcgcccccg ggcaagctcg gtcaactgcg ccggcggcaa gggcctcatc tgtcgggggt ctccctggcg ccaacgtcgc gatcgccgtg gggacgacga gttcgggcac acggcatcaa cttcgaccag <210> 294 <211> 329 <212> DNA <213> Pinus radiata <400> 294 cagagcttgc atgtggatct cttatttcag atccttgcaa ggtgttattc tttcatatga gcccgggcgg gtattttgag gagattgtat ttttaactga tttttcaccc aaatctcaag catcagagag atcagcacat tcctaatttg catttgggat aggtgccgaa ctattgctg gaaaaaatt t ttggctgcaa aggctgccgt tctgcagacg tccttgtgat tccactatgg tcaagatagc tatggccatc ttataaagct gaatgctgtt ttcaataagc aaaagatgct agaagatgca aagtgagcaa g ta cg ta aa c 120 '180 240 300 329 <210> <211> <212> <213> 295 496
DNA
Pinus radiata <400> 295 gtcgttgctt cttactgcat gaaaagcctg agaagagctt acggtctcgg atgtttcgct cctgctaatg tggctgttgg gttggggatg atgagtttgg gaccgaggaa ttagatttga atgaatggcg agagggaatt gaatctgagc ttgatgcaga agtctgattg cagagc tgagtcattg gatcgtttgc ggctgaagcg aatttccagg gcgcatgctt ttcccatgcc tatgttctat gctgatccga gaaaatggtt tttggggaga cccggattcc ctcggtggcc gctgacattc agaaccgcgc cgtaatccca gaggcatcga tcagaaaggc tgctcattga aaaaggctgc gatccgcatt tgagggaaaa tggcattcgt gcgctgacat tatttcacta tctcaatttg ttttgtcccc aggtggtgca tgttggcaag caatgtgatg tactttaaag gcttctcaag tggatcaatc 120 180 240 300 360 420 480 496 <210> <211> <212> <213> 296 473
DNA
Pinus radiata <400> 296 gaycagctga caggtgaatc gtcccaacag ttgccggatt ggtgcacctg ctaatgttgc ggaaaggttg gtgatgacga gtaaataata agggcatgcg ttaaggagtg atggtgaacg ctcgacgaat cagagcttga tcaataagcc ttatttcaga accacttgtg gt cat tatc t tgtttgcata gtttggatat ttttgatgct tgaatttatg tgtggatctc tccttgcaaa gtttgctttg gaagcgcagg gcaagactag atgcttgctg ggagctcgaa ttttacagaa atcagagagg tcagcacatt gagaaatgct ccttcaaaaa gaggttcatc atatcttgga ctgctttggc atccaagtgc caaaaatttt tggctgcaat gattgatttc ggctcctgga agcatttatt gaaaaacaat atttgtgaca agatatgtta ccactatggt caa <210> 297 WO 00/22092 WO 0022092PCT/NZ99/00 169 <211> 369 <212> DNA <213> Pinus radiata <400> 297 cagcagacag gtgaatcacc ccaacagttg ccggattgtc gcacctgcta atgttgctgt aaggttggtg atgacgagtt aataataagg gcatgcgttt aggagtgatg gtgaacgtga gacgaatca acttgtggtt attatctgaa ttgcatagca tggatatatg tgatgctgga atttatgttt tgctttggag gcgcaggcct agactaggag cttgctgata gctcgaactg tacagaaatc aaatgctgat tcaaaaaggc gttcatcagc tcttggagaa ctttggcatt caagtgcaga tgatttcgtc tcctggaggt atttattgga aaacaatgta tgtgacatta tatgttactc 120 180 240 300 360 369 <210> <211> <212> <213> 298 459
DNA
Pinus radiata <400> 298 gagcagctga caggtgaatc gtcccaacag ttgccggatt ggtgcacctg ctaatgttgc ggaaaggttg gtgatgacga gtaaataata agggcatgcg ttaaggagtg atggtgaacg ctcgacgaat cagagcttga tcaataagcc ttatttcaga accacttgtg gtcattatct tgtttgcata gtttggatat ttttgatgct tgaatttatg tgtggatctc tccttgcaaa gtttgctttg gaagcgcagg gcaagactag atgcttgctg ggagctcgaa ttttacagaa atcagagagg tcagcacat gagaaatgct ccttcaaaaa gaggttcatc atatcttgga ctgctttggc atccaagtgc caaaaatttt gattgatttc ggctcctgga agcatttatt gaaaaacaat atttgtgaca agatatgtta ccactatggt 120 180 240 300 360 420 459 <210> 299 <211> 417 <212> DNA <213> Pinus radiata <400> 299 gttctgactc agattgcaag catctcctgc acctgttgta aagactttgt atagtatcgg tggaatgcca gagtgacatc ccacctcttc atcacttatc tgcgctggtg ccgaattcgg aatggaaaaa acttggagga tgacatgtct tcaattgcag cagcctttgg accacttcgt tttatcaaat cacgaggcag catggaggaa at tCt tgagg tcactgataa gtccatcagt cattatcaga ccgcttcctc agcgggtgaa aactctttaa atgaacacct tgtatctacc tactagaagt gtctcctccc ccaaatgctg ttttctgttt gattcctgaa cccacaaatg tttccgcgaa aattttaatt gtcaggaatg agaatgcctt gggggtagga gcactca 120 180 240 300 360 417 <210> <211> <212> <213> 300 359
DNA
Pinus radiata <400> 300 gtccgtgatc agactaattg gtcaagctca gattcagtta tcgttcacca tcggctttca gcgcatccct ttgtgctcca ttcatcctcg ccaaccttcc ggcaacattc gcaggggctc atccataatq gtagcatatc atgtgacaaa cattattttc ctataaatgc cccctggagc gaaaatttta agcactggga agccaaagca tttcagaata ggatgaacct tttcttgaat gcCtgttgga ttccggaaaa gttcgagcac tccacaagca ccgagccttg gcaggagcat ttaagtccac acataaactc caagatcaaa tgcgcccaaa ctataccaac cagccaatg <210> 301 <211> 374 WO 00/22092PC/Z/OI6 PCT/NZ99/00169 <212> DNA <213> Pinus radiata <400> 301 gtccgtgatc agactaattg gtcaagctca gattcagtta tcgttcacca tcggctttca gcgcatccct ttgtgctcca ttcatccicg ccaaccttcc ggcaacattc gcaggggctc cactccattg accg atccataatg gtagcatatc atgtgacaaa cattattttc ctataaatgc cccctggagc gaaaatttta agcactggga agccaaagca tttcagaata ggatgaacct tttcttgaat gcctgttgga ttccggaaaa gttcgagcac tccacaagca ccgagccttg gcaggagcat ttaagtccac acataaactc caagatcaaa tgcgcccaaa ctataccaac cagccaatga 120 180 240 300 360 374 <210> <211> <212> <213> 302 339
DNA
Pinus radiata <400> 302 gtccatcagt tactagaagt cattatcaga gtctcctccc ccgcttcctc ccaaatgctg gcaatctcac attcgggtca ctgcaagatg agctgaccta tcgatgcctc tcggatcagc aattttaatt gtcaggaatg agaatgcctt taggagagca gttggctctg tctgcatcaa tggaatgcca gagtgacatc accacttcgt ccacctcttc atcacttatc tttatcaaat tgcgagcctc atcatcagat ggccaaagag gggcacctcc ttttctcgca attttcatgg caatcagact gattgatcca tagtgaaata gctcagatt 120 180 240 300 339 <210> 303 <211> 402 <212> DNA <213> Pinus radiata <400> 303 gtccatcagt tactagaagt cattatcaga gtctcctccc ccgcttcctc ccaaatgctg gcaatctcac attcgggtca ctgcaagatg agctgaccta tcgatgcctc tcggatcagc tgggattacg atagaacata aattttaatt gtcaggaatg agaatgcctt taggagagca gttggctctg tctgcatcaa aattccctct tggaatgcca ccacctcttc tgcgagcctc gggcacctcc caatcagact gctcagattc cgccattcat gagtgacatc atcacttatc atcatcagat ttttctcgca gattgatcca cttgagaagc ct accacttcgt tttatcaaat ggccaaagag attttcatgg tagtgaaata atgtcagcgc 120 180 240 300 360 402 <210> <211> <212> <213> 304 468
DNA
Pinus radiata <400> 304 gcqaatqttq ccattggtat ggcgaggatg aatttgggcg aaagggatgc gctttgatct gatggtgaac gagagtttat tctgagcttg acgtggactt ctgatcacgg acccctgtaa ggcagtatac tgtcttatga gctcgggagg gtattttaag aqcaacqqctc catgcttgtg tggtgct cga gtttttccgg aatccaacag gtctgcgcat tcccaatctc catatgggat oqaqqttcat gatattctga actgctttgg aatcccagtg gctaaaattt ttggctgcca agattgccat aaagcagatt cccicatttat aagaaaataa cttttgtcac ctgatatgct tccattatgg tgaaaatcgc tatggccatc taattaag aqqqaaqqtt tgtggagcac attgaaagcc actaactgaa atcaattagt tagagacacq agcgagcgaa 120 180 240 300 360 420 468 <220> 305 <211> 502 <212> DNA WO 00/22092PC/Z9016 PCT/NZ99/00169 <213> Pinus radiata <400> 305 gcgaatgttg ccgttggtat ggcgaggatg aatttgggcg aaagggatgc gctttgatct gatggtgaac gagagtttat tctgagcttg acgtggactt ctgatcacgg acccctgtaa ggcagtatac tgtcttatga gctcgggagg gtattttaag gaagtaggat ttttaacagg agcaaggctc catgcttgtg tggtgctcga gtttttccgg aatccaacag gtctgcgcat tcccaatctc catatgggat ag ggaggttcat gatattctga actgc t ttgg aatcccagtg gctaaaattt ttggctgcca agattgccat aaagcagatt ccgcatttat aagaaaataa cttttgtcac ctgatatgct tccattatgg tgaaaatcgc tatggccatc taat taaggt agggaaggt t tgtggagcac attgaaagcc actaactgaa atcaattagt tagagacacg agcgagcgaa tagtgaagag 120 180 240 300 360 420 480 502 <210> 306 <211> 379 <212> DNA <213> Pinus radiata <400> 306 gaactgcttt ggcatttgtg gaaatccaag tgcagatatg aggcaaaaat tttccactat atttggctgc aatcaagata tgaggctgcc gttatggcca attctgcaga cgttataaag atccttgtga tgatgctgt acattaagga ttactcgacg ggttcaataa gcaaaagatg tcagaagatg ctaagtgagc gtgatggtga aatcagagct gccttatttc ctggtgttat cagcccgggc aagagattgt acgtgaattt tgatgtggat agatccttgc tctttcatat gggt at t ttg atttttaact atgttttaca ctcatcagag aaatcagcac gatcctaatt agcatttggg gaaggtgaag 120 180 240 300 360 379 <210> 307 <211> 233 <212> DNA <213> Pinus radiata <400> 307 agtttatgtt tttccggaat cccagtgctg atatgctact aactgaatct gagcttgacg tggacttaat ccaacaggct aaaattttcc attatggatc aattagtctg atcacggacc cctgtaagtc tgcgcatttg gctgccatga aaatcgctag agacacgggc agtatactgt cttatgatcc caatctcaga ttgccattat ggccatcagc gagccgaagc tcg <210> 308 <211> 377 <212> DNA <213> Pinus radiata 120 180 233 <400> 308 gaaagctcca gggggagccc atccgcattt atagggaagg gaaagaaaat aatgtggagc ggcttttgtc acattgaaag tgctgatatg ctactaactg ttttccatta tggatcaatt ccatgaaaat cgctaga ctgcgaatgt ttggcgagga acaaagggat ccgatggtga aatctgagct aatctgatca tgccgttggt tgaatttggg gcgctttgat acgagagttt tgaccgtgga cggacccctg atagcaaggc cgcatgcttg cttggtgctc atgtttttcc cttaatccaa taagtctgcg tcggaggttc tggatattct gaactgcttt ggaatcccag caggctaaaa catttggctq 120 180 240 300 360 377 <210> 309 <211> 517 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 309 gtcccaacag ttgccggatt ggt-gcacctlg ctaatgttgc ggaaaggttg gtgatgacga gtaaataata agggcatgcg ttaaggagtg atggtgaacg ctcgacgaat cagagcttga tcaataagcc ttatttcaaa aaagatgctg gtgttattct gatgcacccg ggcggtattt gtcattatct tgtttgcata gtttggatat ttttgatgct tgaatttatg tgtggatctc tccttgcaaa ttcatatgat t tgac tt tgg gaagcgcagg gcaagactag atgcttgctg ggagctcgaa ttttacagaa atcagagagg tcagcacatt cctaatttga gattctt ccttcaaaaa gaggttcatc atatcttgga ctgctttggc atccaagtgc caaaaatttt tggctgcaat gctgccgtat ggctcctgga agcatttatt gaaaaacaat atttgtgaca agatatgtta ccactatggt caagatagca ggcatcaaaa <210> 310 <211> 360 <212> DNA <213> Pinus radiata <400> 310 gttgtacgga accttttcca ggatgtagat attacaccaa gtagacacca ctggtgctgg gacctcactt tattgcagaa gtgcaattac ggttactgag tagcagctct gacaaaagtt tccaaacctc ggattttagc agatgcgttt gagagcctga aggggggcaa cttgcctgag aaattgcttc ggaagagtga gtgagtggaa gagaggcatt tccctgctct ctttttatct tcgtgactga aaggcctggc ttcttagtca gaagtttgca tcccacacga acatttcttt aggccagcaa agtggaagct attggctaag aatgcctgtg gaagcagtgc ttttgcatag <210> 311 <211> 438 <212> DNA <213> Pinus radiata <400> 311 cggccggctt caggtttaat tctgaggtgt ccccgctagt gtcaatggag tgtcattggc gcgaatgttg ccgttggtat ggcgaggatg aatttgggcg aaagggatgc gctttgatct gatggtgaac gagaagttta atctgacttg acgtggac agtgaaagca ggtgtgcttt tgatgctcct agcaaggctc catgcttgtg tggtgctcga tgtttttccg t tgagtgaag ggggaattgc gcattcaaga ggaggttcat gatattctga actgctttgg gaatcccagt acgacaataa tgatcgattt aagctccagg ccgcatttat aagaaaataa cttttgtcac gctgatatgc tgggaaacca tgtcccaacg gggagcccct agggaaggtt tgtggagcac attgaaagcc tactaactga 120 180 240 300 360 420 438 <210> 312 <211> 294 <212> DNA <213> Pinus radiata <400> 312 gtgaacgtga atttatgttt agcttgatgt 9gaLcicatc tttcagatcc ttgcaaatca ttattctttc atatgatcct gggcgggtat tttgagcatt tacagaaatc agagaggcaa gcacatttgg aatttgaggc tgggattctg caagtgcaga tatgttactc aaattttcca ctatggttca ctgcaatcaa gatagcaaaa tgccgttatg gccatcagaa cagacgttat aaagctaagt gacgaat cag ataagcctta gatgctggtg gatgcagccc gagc 120 180 240 294 <210> 313 <211> 510 <212> DNA <213> Pinus radiata <400> 313 WO 00/22092 WO 0022092PCT/NZ99/00I 69 tcaaggttca acacaaaagg aataccttcg aacggccggc cattgagtga ttggggaatt ctgcat tcaa tcggaggttc tggatattct gaactgcttt ggaatcccag aggctaaaat agacgacaat gctgatcgat gaaagctcca atccgcattt gaaagaaaat ggcttttgtc tgctgatatg tttccattat aatgggaaac L L gtcccaa gggggagccc atagggaagg aatgtggagc acattgaaag ctactaactg ggatcaatta catctgaggt cgg tcaatgg ctgcgaatgt ttggcgagga acaaagggat ccgatggtga aatctgagct ttcaggttta gtCCCCgCta agtgtcazrg tgccgttggt tgaatttggg gcgctttgat acgagagttt tgacgtggac atagtgaaag gtggtgtgct gctgatgctc atagcaaggc cgcatgcttg cttggtgctc atgtttttcc ttaatccaac <210> 314 <211> 487 <212> DNA <213> Pinus radiata <400> 314 ttttcgttca tcagttagat attcgacaat cgtcgcatcg gcaacggagg aggtgaatca tgccggattg taatgttgct tgatgac ttatattcat tgtaagtttg aatgtcgaga ggccgcctcg tctcaaggtt ccacttgtgg tcattatctg gtttgcatag tgccatcata cggccatggc aatctgcacc cgaaggtitaa ctgcaagagc tttgctttgg aagcgcaggc caagactagg ttcctgttct tatgcgcgtt cccgctgacg tcgatcggct atcagtagca agaaatgctg cttcaaaaag agggtcatcn tgtctgtcct ccggcaaatt aattcgagca tcgttgtcgc gacaacaaag attgatttcg gctcctggag gcntttattg atcacattat ccattgctcc catcgtcatt tcactatteg agcagctgac tcccaacagt gtgcacctgc gaaaggt tgg 120 180 240 300 360 420 480 487 <210> 314 <211> 421 <212> DNA <213> Pinus radiata <400> 315 cttcaggttt aatagtgaaa tgtccccgct agtggtgtgc gagtgtcatt ggctgatgct ttgccgttgg tatagcaagg atgaatttgg gcgcatgctt tgcgctttga tcttggtgct aacgagagtt tatgtttttc t gcattgagtg tttggggaat cctgcattca ctcggaggtt gtggatattc cgaactgctt cggaatccca aagacgacaa tgctgatcga agaaagct cc catccgcatt tgaaagaaaa tggcttttgt gtgctgatat taatgggaaa ttttgtccca agggggagcc tatagggaag taatgtggag cacattgaaa gctactaact ccatctgagg acggtcaatg cctgcgaatg gttggcgagg cacaaaggga gccgatggtg gaatctgagc 120 180 240 300 360 420 421 <210> 316 <211> 420 <212> DNA <213> Pinus radiata <400> 316 ggacccctgt aagtctgcgc actgtcttat gatcccaatc gggtatttta aacatatggg atttttaaca gqaggtqcag aaacctcaaa ttgcttctcg ttttagcgga agagtgaaag tgcgtttgtg agtggaattc atttggctgc tcagattgcc ataaagcaga 0 L- C C tgactgaagg gcctggcagt ttagtcaatt catgaaaatc attatggcca tttaattaag cgacactgtt ccagcaagga ggaagctgta ggctaaggac gctagagaca t cagcgagcg gttagtgaag ytacgcaacc tgtagatatt gacaccactg ctcactttat cgggcagtat aagctcggga aggaagtagg ttttccatcc acaccaagga gtgctggaga tgcagaaaga 120 180 240 300 360 420 <210> 317 <211> 499 WO 00/22092PC/Z9016 PCT/NZ99/00169 <212> DNA <213> Pinus radiata <400> 317 ctgcactgag tcattggaaa gagcttgatc gtttgctttg ttcgttggct gaagcgcccg tgttggaatt tccaggctcg gtttgggcgc atgcttgctg atttgattcc catgccagaa ggaatttatg ttctatcgta t9caaagctg atcccagagg gcccactagg tcagctcat atggtttcag gggagatgct gattccaaaa gtggccgatc acattctgag ccgcgctggc atcccagcgc catcgatatt aaaggctctc cattgatttt ggctgcaggt cgcatttgtt ggaaaacaat attcgttact tgacatgctt tcattatgga aatttggaaa gtccccacgg ggtgcacctg ggcaaggttg gtgatggacc ttaaagatga ctcaaggaat tcaatcagtc agcctgagaa tctcggatgt ctaatgtggc gggatgatga gaggaattag atggcgagag ctgagcttga tgattgcaga 120 180 240 300 360 420 480 499 <210> <211> <212> <213> 318 364
DNA
Pinus radiata <400> 318 gcgaagctcg ggagggtatt aagaggaagt aggattttta accttttcca tccaaacctc attacaccaa ggattttagc ctggtgctgg agatgcgttt tattgcagaa agaagagggc ttac ttaagcatat acaggaggtg aaattgcttc ggaagagtga gtgagtggaa ctgagagagg gggataaagc cagatccatt tcgtgactga aaggcctggc ttcttagtca cattgaagtt agatttaatt cgacgacact aggccagcaa agtggaagct attggctaag tgcaaatgcc aaggttagtg gttgtacgca ggatgtagat gtaaacacca gacctcactt tgtggtgcaa <210> 319 <211> 298 <212> DNA <213> Eucalyptus grandis <400> 319 aagcgattcg tttcttcgag ctcaacaccg gggccaagat cCCctccgtc gggctgggca cttggcagac cggtgacggc gtcgacgccg tcaccaccgc catcaaggtt gggtacaggc atattgattg tgctcaagct tatcaaaatg agaaggagat tggtactgct ctccagaaat tattcagcga gggtgtggtg aagcgcgagg atttgtggat cacatccaag ctatggtgtg ctgatcacgc accagaaaga tgttcccaag gcatttagaa agaaccctgg agaaactt <210> 320 <211> 261 <212> DNA <213> Eucalyptus grandis <400> 320 gttttcgaga gagaaatqqc gaa gqcqatL cgtttcttcg agctcaacac cggggccaag atcccctccg tcgggctggg cacttggcag accggtgacg gcgtcgacgc cgtcaccacc gccatcaagy ttgggtacag gcatattgat tgtgctcaag cttatcaaaa tgagaaggag attggtactg ctctccagaa attattcagc gagggtgtgg tgaagcgcga ggatttgtgg atcanatc a'ctaggtq t 120 180 240 298 120 180 240 261 <210> 321 <211> 450 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 321 ctagggtgag atcgaagctc cgagctcaac accggggcca cggcgtcgac gccgtcacca agcttatcaa aatgagaagg ggtgaagcgc gaggatttgt agatgttccc aaggcattag ttacctgatc cactggccgg ccttacccag ccggacatac gcgttttcga agatcccctc ccgccatcaa agattggtac ggatcacatc aaagaaccct tgagcatgag ccagtacgtg gagagaaatg cgtcgggctg ggttgggtac tgctctccag caagctatgg ggagaacttg gaaggctcaa gcgaaggcga ggcacttggc aggcatattg aaattattca tgtgctgatc cagctcgagt tttggcttca ttcgtttctt agaccggtga attgtgctca gcgagggtgt acgcaccaga atctggatct agcctgaaaa <210> 322 <211> 347 <212> DNA <213> Eucalyptus grandis <400> 322 cgagagagaa atggcgaagg ctccgtcggg ctgggcactt caaggttggg tacaggcata tactgctctc cagaaattat atccaagcta tggtgtgctg cctggagaac ttgcagctcg cgattcgttt ggcagaccgg ttgattgtgc tcagcgaggg atcacgcacc agtatctgga cttcgagctc tgacggcgtc tcaagcttat tgtggtgaag agaagatgtt tctttacctg aacaccgggg gacgccgtca caaaatgaga cgcgaggat t cccaaggcat atccact ccaagatccc ccaccgccat aggagattgg tgtggatcac tagaaagaac <210> <211> <212> <213> 323 414
DNA
Eucalyptus grandis <400> 323 cacgagtcgg ctggcgaagt gaccgagtcg ctatcgaggc actggccgat acaacgcatg acattgacgc gctggcacct tctttcgaag agggcgcctt tccggtctca gactcggaga tctctacttt cggcccgtgc agtcgaagta tggagtacct cccagatgtc tcatccggca gtgcgaacca tcccgtcctt tgcgggtgca ggtgaaggcg tgttcccaac gttttcttct cagtggttgc ctcgctgtcg gtctgtggtg gagcctattg taacccaatg ctgcttgcga caaccccgcc accgtcttcc cattggccgg ctggaccaat ttataacgga gaaagt cggc tgcgtgtcgt gttccatggc ggataatgtt catcgagcgt aggcctaatc cctt <210> 324 <211> 464 <212> DNA <213> Eucalyptus grandis <400> 324 cacgagtcgg ctggcgaagt gaccgagtcg ctatcgaggc actggccgat acaacgcatg acattgacgc gctggcacct tctttcgaag agggcgcctt tccggtctca gactcggaga tctctacttt cggcccgtgc agccgtctgg actttgcgaa agtcgaagta tggagtacct cccagatgtc tcatccggca gtgcgaacca tcccgtcctt tgcgggtgca ga agct ggrg ggtgaaggcg tgttcccaac gttttcttct cagtggttgc ctcgctgtcg gtctgtggtg gagcctattg cctggcgttc taacccaatg ctgcttgcga caaccccgcc accgtcttcc cattggccgg ctggaccaat ttataacgga gcac gaaagtcggc tgcgtgtcgt gttccatggc ggataatgtt catcgagcgt aggcctaatc ccttttccaa 120 180 240 300 360 420 464 <210> 325 <211> 368 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/0169 <400> 325 cacgagtcgg ctggcgaagt gaccgagtcg ctatcgaggc actggccgat acaacgcatg acattgacgc gctggcacct tctttcgaag agggcgcctt tccggtctca gactcggaga tctctact agtcgaagta tggagtacct cccagatgtc tcatccggca gtgcgaacca tcccgtcctt ggtgaaggcg tgttcccaac gttttcttct cagtggttgc ctcgctgtcg gtctgtggtg taacccaatq ctgcttgcga caaccccgcc accgtcttcc cattggccgg ctggaccaat gaaagtcggc tgcgtgtCgt gttccatggc ggataatgtt catcgagcgt aggcctaatc <210> <211> <212> <213> 326 350
DNA
Eucalyptus grandis <400> 326 ctagggtgag atcgaagctc cgagctcaac accggggcca cggcgtcgac gccgtcacca agcttatcaa aatgagaagg ggtgaagcgc gaggatttgt agatgttccc aaggcattag gcgttttcga agatcccctc ccgccatcaa agattggtac ggatcacatc aaagaaccct gagagaaatg cgtcgggctg ggttgggtac tgctctccag caagctatgg ggagaacttg gcgaaggcga ttcgtttctt ggcacttggc agaccggtga aggcatattg attgtgctca aaattattca gcgagggtgt tgtgctgatc acgcaccaga cagctcgagt 120 180 240 300 350 <210> 327 <211> 372 <212> DNA <213> Eucalyptus grandis <400> 327 cttcgagctc aacaccgggg tgacggcgtc gacgccgtca tcaagcttat caaaatgaga tgtggtgaag cgcgaggatt agaagatgtt cccaaggcat tctttacctg atccactggc aaaccttacc ca ccaagatccc ccaccgccat aggagattgg tgtggatcac tagaaagaac cggtgagcat ctccgtcggg caaggttggg tactgctctc atccaagcta cctggagaac gaggaagggc ctgggcactt tacaggcata cagaaattat tggtgtgctg ttgcagctcg tcaattggct ggcagaccgg ttgattgtgc tcagcgaggg atcacgcacc agtatctgga tcaagcctga <210> <211> <212> <213> 328 333
DNA
Eucalyptus grandis <400> 328 aaatggcgaa ggcgattcgt ggctgggcac ttggcagacc ggtacaggca tattgattgt tccagaaatt attcagcgag tatggtgtgc tgatcacgca acttgcagct cgagtatctg ttcttcgagc ggtgacggcg gctcaagctt ggtgtggtga ccagaagatg gatctttacc tcaacaccgg ggccaagatc tcgacgccgt caccaccgcc atcaaaatga gaaggagatt agcgcgagga tttgtggatc ttcccaaggc attagaaaga tga ccctccgtcg atcaaggttg ggtactgctc acatccaagc accctggaga 120 180 240 300 333 <210> 329 <211> 377 <212> DNA <213> Eucalyptus grandis <400> 329 cgcactcctt ttgcctgccc cccacgagcc gcgttttcga gagagaaatg gcgaaggcga cogagcggga gtagactgag atcgaagctc ttcgtttctt cgagctcaac acgggggcca 120 WO 00/22092PC/Z/016 PCT/NZ99/00169 agatcccctc catcgggctg ggcacttggc tcaccaccgc cacaaaggct gggtacaggc agaaggagat tggtactgct ctccagaaat atttgtggat cacttccaag ctatggtgta caatagacag aaccttg aggccgatcc cggcgtcgtg gccgaggccg atattgattg tgCtcaagct tattacaatg tattcagcga gggtgtggtg aagcgcgagg ctgatcacgc accggaagat gttcccaagg <210> 330 <211> 484 <212> DNA <213> Eucalyptus grandis <400> 330 aactaatggt tcgcttgcca ggaaaatgtg agcttggagg ttgtcgccga gcaaatatca tggccttgtt accttattag tgtagacgag tgcagattat ctcaacagat gttcagctag cgacattgat gtgagcttcg tgcgactcgt gctggtggca tcct ataaggtggt aaggagcaat atcctgagac cagcccgtgc cgattgcgaa tggatgaaga attgtgttgg aagtgtgcct gcatcctgca gtgtgaaccc caacatactc ttttggagct aatgcttggt agtggcgcgc ctatgacaag catcggacta catctgtgtt ctcagtgttg ataataggat ccgagaatcg gcctctgagg atccaaaatg acaatgacca gccttgagca acaagctacc gtgtacacgc cagggccgat tcatcactga tggttcaagt caatgggctg cagctttgaa agatgacagt <210> 331 <211> 477 <212> DNA <213> Pinus radiata <400> 331 ccaaagggaa aaaaaatggg gacaatatgg ctgcatggct ccgcctcttg gcccccatga gacgtccact atttgaggac attggtcatg agtctgctgg cctggtgacc gcgtactttg gaggctccta caatttgtgt ccttggccaa tcagattgtt gaagggagca cactggaata tgcgaaggtg attacggtgt aataattgag gagcctggaa cccgagatga catcctgcag atgtctcagg aacactcttc cgcatgaag gcggacttta gaggttggca tat cgtgt tg agttttttgc atttatgttt gtaacgaaaa gcatccagcc ctgtgggtat ttgtaaaaga gtgaagtgaa gcgttgtgac aacacctccc caagttgcca tggggaaggt cttcaaactt ctgtggcagt gccaatggtg acatctggtt caatgtaagc gtgcatggt t gataatg 120 180 240 300 360 420 477 <210> <211> <212> <213> 332 433
DNA
Pinus radiata <400> 332 agggtaacga aaatggggaa ttcgcatcca gcccttcaaa nggctgtggg tat.ctgtggc ttattgtaaa anagccaarg gcagtgaagt gaaacatctg ttggcgttgt oaccaatgta tgcaacacct cccgtgcatg hL..OOaagt cca ggtgacaata cttccgcctc aotgacgtcc gtgattggtc gztcctggtg agcnaggctc gttccttggc tggctgcatg ttggccccca actatttgan atgagtctgc accgcgtact ctacaatttg caatcagatt gctcactgga tgatgcgaag gacattacgg tggaataatt ttggagcctg tgtcccgaga gttcatcctg ataaacactc gtgcgcatga tgtgcggact gaggaggttg gaatatcgtq tgaagttttt cagatttatg <210> 333 <211> 466 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 333 gaggaatagg aacaggaccc atgtcaggag caqctgctgc cttggagtgt ggagaatgga atagggtacc gtcatttcga gcacttgcgg aggcctttca aagctatgga attcagacca ctgcagttgg aatatttgga ggggttggaa caactgatag tcttttgttg gattacactg gggtcaggaa ttgtgcagct gcaaggcttg tggacatgtt cctgtacttg tgccttagac ggcacacttg aataatggcc ataagagacc gattacagga gtgaaacgag cttgaggcat gttcattttc gaagatggtg catctgtctt acaaaatgcc ttatcttcaa atgaaaagga aggatatttt gcaaggacag caatagccac tt Ct Cg tgttccagca catcattggg tgcactacac agttggtcaa tattactacc tttaaagaat acgacataca 120 180 240 300 360 420 466 <210> 334 <211> 483 <212> DNA <213> Pinus radiata <400> 334 gggtaacgaa tcgcatccag ggctgtgggt tattgtaaaa cagtgaagtg ttggcgttgt tgcaacacct tttcaagttg tgg aatggggaag cccttcaaac atctgtggca gagccaatgg aaacatctgg gaccaatgta cccgtgcatg ccagataatg gtgacaatat ttccgcctct gtgacgtcca tgattggtca ttcctggtga agcgaggctc gttccttggc taagtctcga ggctgcatgg t gg cc cat ctatttgagg tgagtctgct ccgcgtagct ctacaatttg caatcagatt ggaaggtgcc ctcactggaa gatgcgaagg acattacggt ggaataattg ttggagcctg tgtcccgaga gttcatcctg atgtgtgaac taaacactct tgcgcatgaa gtgcggactt aggaggttgg gaatatcgtg tgaagttttt cagatttatg cactcagtgt 120 180 240 300 360 420 480 483 <210> <211> <212> <213> 335 329
DNA
Pinus radiata <400> 335 gggtaacgaa aatggggaag tcgcatccag cccttcaaac ggctgtgggt atctgtggca tattgtaaaa gagccaatgg cagtgaagtg aaacatctgg ttggcgttgn gaccaatgta gtgacaatat ttCCgcCtct gngacgtcca tgattggtca ttcctggtga agcgaggct ggctgcatgg tggcccccat ctatttgagg tgagtctgct ccgcgtagct ct cact gga a gatgcgaagg acattacggt ggaataattg ttggagcctg taaacactct tgcgcatgaa gtgcggactt aggaggttgg gaatatcgng 120 180 240 300 329 <210> <211> <212> <213> 336 419
DNA
Pinus radiata <400> 336 ctcagggtaa cgaaaatggg tgaaggctgt gggtatctgt actttattgt aaaagagcca ttggcagtga agtgaaacat cgtgttggcg ttgtgaccaa tttttgcaac acctcccgtg gaaggtgaca aaacttccgc ggcagtgacq atggtgattg ctggttcctg catogttcct atatggctgc ctcttggccc tccactattt gtcatgagtc gtgaccgcgt gctcctacaa tggccaatca atggctcact ccatgatgcg gaggacatta tgctggaata agctttggag LtLgtgtccc gattgttcat ggaataaaca aaggtgcgca cggtgtgcgg at tgaggagg cctggaatat gagatgaagt cctgcagat 120 180 240 300 360 419 <210> 337 <211> 392 <212> DNA <213> Pinus radiata WO 00/22092PC/Z9016 PCT/NZ99/00169 <400> 337 ctcagggtaa cgaaaatggg ctcttCgcat ccagcccttc tgaaggctgt gggtatctgt actttattgt aaaagagcca ttggcagtga agtgaaacat cgtgttggcg ttgtgaccaa tttttgcaac acctcccgtg gaaggtgaca aaacttccgc ggcagtgacg atggtgattg ctggttcctg tgtaagcgag catggttcct atatggcrgc Ctcttggccc tccactattt gtcatgagtc gtgaccgcgt gctcctacaa tg atggctcact ccatgatgcg gaggacatta tgctggaata agctttggag tttgtgtccc ggaataaaca aaggtgcgca cggtgtgcgg at tgaggagg cctggaatat gagatgaagt 120 180 240 300 360 392 <210> <211> <212> <213> 338 362
DNA
Pinus radiata <400> 338 ctaaccaaag ggaaaaaaaa aggtgacaat atggctgcat acttccgcct cttggccccc cagtgacgtc cactatttga ggtgattggt catgagtctg ggttcctggt gaccgcgtag gt tggggaaggg ggctcactgg atgatgcgaa ggacattacg ctggaataat ctttggagcc agcaatgtct aataaacact ggtgcgcatg gtgtgcggac tgaggaggtt tggaa tat cq cagggtaacg cttcgcatcc aaggctgtgg tttattgtaa ggcagtgaag tgttggccgt aaaatgggga agcccttcaa gtatctgtgg aagagccaat tgaaacatct tgtgaccaat <210> 339 <211> 417 <212> DNA <213> Pinus radiata <400> 339 aaaaaaatgg ggaagggagc gctgcatggc tcactggaat ggcccccatg atgcgaaggt tatttgagga cattacggtg gagtctgctg gaataattga cgcgtagctt tggagcctgg tacaatttgt gtcccgagat aatgtctcag aaacactctt gcgcatgaag tgcggacttt ggaggttggc aatatcgtgt gaagtttttt ggtaacgaaa cgcatccagc gctgtgggta attgtaaaag agtgaagtga tggcgttgtg gcaacacctc atggggaagg ccttcaaact tctgtggcag agccaatggt aacatctggt accaatgtaa ccgtgcatgg tgacaatatg tccgcctctt tgacgtccac gattggtcat tcctggtgac gcgaggctcc ttccttg 120 180 240 300 360 417 <210> 340 <211> 343 <212> DNA <213> Pinus radiata <400> 340 ccaaagggaa aaaaatgggg acaatatggc tgcatggctc cgcctgcccta acatccacta tttgaggaca ttggtcatga gtctgctgga ctogtgaccg cgtagctttg aagggagcaa actggaataa gcgaaggtgc ttacggtgtg ataattgagg gagcctggaa tgtctcaggg acactcttcg gcatgaaggc cggactttat aggttggcag tatcgtgttg taacgaaaat ggggaaggtg catccagccc ttcaaacttc tgtgggtatc tgtggcagtg tgtaaaagag ccaatggtga tgaagtgaaa catctggttc gc9 120 180 240 300 343 <210> 341 <211> 590 <212> DNA <213> Pinus radiata <400> 341 WO 00/22092 WO 0022092PCT/NZ99/00I 69 attggtcatg cctggtgacc cgaggctcct tccttggcca agtctcgagg gcttctgtag accgtgctgt gagcgtctgt tctcaggata gtaacttttg agtctgctgg gcgtagcttt acaatttgtg atcagattgt aaggtgccat gccctgagac ctgcacgtgc caatggctaa ttgatgaaga attgtgctgg aataattgag ggagcctgga tcccgagatg tcatcctgca gtgtgaacca aaatgtcttg atttggagct aaaggt tggc agtgacccgc ttttgctaaa gaggttggca atatcgtgtt aagttttttg gatttatgtt ctcagtgttg gtaatggggg tcacgaatta tccgatgaat atacaaaatg accatgtcga gtgaagtgaa ggcgttgtga caacacctcc tcaagttgcc gggttcatgc caggtcctat ttattgctga gcgtcttagt ccatgggtgg cggctctaaa acatctggtt ccaatgiaag cgtgcatggt agataatgta ttgtcgccgt cggccttgtc tgtagatgaa ctccagagac aaacatagat <210> 342 <211> 372 <212> DNA <213> Pinus radiata <400> 342 atctaaccaa agggaaaaaa aaggtgacaa tatggctgca aacttccgcc tcttggcccc gcagtgacgt ccactatttg tggtgattgg tcatgagtct tggttcctgg tgaccgcgta gtaagcgagg ct atggggaaag tggctcactg catgatgcga aggacattac gctggaataa gctttggagc gagcaatgtc gaataaacac aggtgcgcat ggtgtgcgga ttgaggaggt ctggaatatc tcagggtaac tcttcgcatc gaaggctgtg ctttattgta tggcagtgaa gtgttggcgt gaaaatgggg cagcccttca ggtatctgtg aaagagccaa gtgaaacatc tgtgaccaat 120 180 240 300 360 372 <210> <211> <212> <213> 343 378
DNA
Pinus radiata <400> 343 gtggcagtga cgtccactat caatggtgat tggtcatgag atctggttcc tggtgaccgc aatgtaagcg aggctcctac tgcatggttc cttggccaat ataatgtaag tctcgaggaa gtcgcccgtg cttctgta ttgaggacat tctgctggaa gtagctttgg aatttgtgtc cagattgttc ggtgccatgt tacggtgtgc taattgagga agcctggaat ccgagatgaa atcctgcaga gtgaaccact ggactttatt ggttggcagt atcgtgttgg gttttttgca tttatgtttc cagtgttggg gtaaaagagc gaagtgaaac cgttgtgacc acacctcccg aagttgccag gttcatgctt <210> 344 <211> 510 <212> DNA <213> Pinus radiata <400> 344 agcaatgtct cagggtaacg aataaacact cttcgcatcc ggtgcgcatg aaggctgtgg gtgtgcggac tttattgtaa tgaggaagtt ggcagtgaag tggaatateg tgttggcgc gatgaagttt tttgcaacac tgcagattta tgtttcaagt accactcagt gttggggttc aaaatgggga agcccttcaa gtatctgtgg aagagccaat tgaaacatct gtgaccaatg ctcccgtgca tgccagataa atgcttgtcg aagtgacaat acttccgcct cagtgacgtc ggtgattggt ggt tcctggt taagcgaggc tggttccttg tgtaagtctc atggctgcat cttggccccc cactatttga catgagtctg gaccgcgtag tcctacaatt gccaatcaga gaagaaggtg ggctcactgg atgatgcgaa ggacattacg ctggaataat ctttggagcc tgtgtcccga ttgttcatcc ccatgtgtga 120 180 240 300 360 420 480 si0 <210> 345 <211> 504 WO 00/22092 WO 0022092PCT/NZ99/00I 69 <212> DNA <213> Pinus radiata <400> 345 gtctcagggt cactcttcgc catgaaggct ggactttatt ggttggcagt atcgtgttgg gttttttgca tttatgtttc cagtgt tgg aacgaaaatg atccagccct qtgggtatct gtaaaagagc gaagtgaaac cgttgtgacc acacctcccg aagttgccag gttcatgctt gggaaggtga tcaaacttcc gtggcagtga caatggtgat atctggttcc aatgtaagcg tgcatggttc ataatgtaag gtcg caatatggct gcctcttggc cgtccactat tggtcatgag tggtgaccgc aggctcctac cttggccaat tctcgaggaa gcatggctca ccccatgatq ttgaggacat tctgctggaa gtagctttgg aatttgtgtc cagattgttc ggtgccatgt ctggaataaa cgaaggtgcg tacggtgtgc taattgagga agcctggaat ccgagatgaa atcctgcaga gtgaaccact ':210> 346 <211> 426 ':212> DNA ':213> Pinus radiata ':400> 346 gcaatgtctc agggtaacga ataaacactc ttcgcatcca gtgcgcatga aggctgtggg tgtgcggact ttattgtaaa gaggaggttg gcagtgaagt ggaatatcgt gttggcgttg atgaagtttt ttgcaacacc gcagat aaatggggaa gcccttcaaa tatctgtggc agagccaatg gaaacatctg tgaccaatgt tcccgtgcat ggtgacaata cttccgcctc agtgacgt cc gtgattggtc gttcctggtg aagcgaggct ggttccttgg tggctgcatg ttggccccca actatttgag atgagtctgc accgcgtagc cctacaattt ccaatcagat gctcactgga tgazgcgaag gacattacgg tggaataatt tttggagcct gtgtcccgag tgttcatcct ':210> 347 ':211> 534 <212> DNA <213> Pinus radiata <400> 347 atttgtgtcc agattgttca gtgccatgtg ctgagacaaa cacgtgcatt tggctaaaaa atgaagaagt gtgctggt tt tgccttgtgg cgagatgaag tcctgcagat tgaaccactc tgtcttggta tggagcttca ggttggctcc gacccgcata tgctaaaacc gaatgggcca ttttttgcaa ttatgtttca agtgttgggg atgggggcag cgaattatta gatgaatgcg caaaatgcca atgtcgacgg taatgagatg cacctcccgt agttgccaga ttcatgcttg gtcctatcgg ttgctgatgt tcttagtctc tgggtggaaa ctctaaagct actgtgccac gcatggttcc taatgtaagt tcgccgtgct ccttgtcacc agatgaagag cagagactct catagatgta acgtctgctg tcactccagc ttggccaatc ctcgaggaag tctgtaggcc gtgctgt ctg cgtctgtcaa caggatattg acttttgatt cggtaaggta tgct 120 180 240 300 360 420 480 534 <210> 348 <211> 352 <212> DNA <213> Pinus radiata <400> 348 gggaagggag caatgtctca ctcactggaa taaacactct gatgcgaagg tgcgcatgaa acattacggt gtgcggactt ggaataattg aggaggttgg tiggagcctg gaatatcgtg gggtaacgaa tcgcatccag ggctgtgggt tattgtaaaa cagtgaagtg ttggcgttgt aatggggaag cccttcaaac atctgtggca gagccaatgg aaacatctgg gaccaatgta gtgacaatat ttccgcctct gtgacgtcca tgattggtca ttcctggtga agcgaggctc ggctgcatg tggcccccat ctatttgagg tgagtctgct ccgcgtagct Ct WO 00/22092PC/Z90I6 PCT/NZ99/00169 <210> 349 <211> 340 <212> DNA <213> Pinus radiata <400> 349 gtctcagggt aacgaaaatg cactcttcgc atccagccct catgaaggct gtgggtatct ggactttatt gtaaaagagc ggttggcagt gaagtgaaac atcgtgttgg cgttgtgacc gggaaggtga tcaaacttcc gtggcagtga caatggtgat atctggttcc aatgtaagcg caatatggct gcctcttggc cgtccactat tggtcatgag tggtgaccgc aggctcctac gcatggctca ccccatgatg ttgaggacat tctgctggaa gtagctttgg ctggaataaa cgaaggtgcg tacggtgtgc taattgagga agcctggaat 120 180 240 300 340 <210> 350 <211> 337 <212> DNA <213> Pinus radiata <400> 350 gcaatgtctc agggtaacga ataaacactc ttcgcatcca gtgcgcatga aggctgtggg tgtgcggact ttattgtaaa gaggaggttg gcagtgaagt ggaatatcgt gttggcgttg aaatggggaa gcccttcaaa tatctgtggc agagccaatg gaaacatctg tgaccaatgt ggtgacaata cttccgcctc agtgacgtcc gtgattggtc gttcctggtg aagcgag tggctgcatg ttggccccca actatttgag atgagtctgc accgcgtagc gctcactgga tgatgcgaag gacattacgg tggaataatt tttggagcct 120 180 240 300 337 <210> <211> <212> <213> 351 500
DNA
Pinus radiata <400> 351 tctcagggta acgaaaatgg actcttcgca tccagccctt atgaaggctg tgggtatctg gactttattg taaaagagcc gttggcagtg aagtgaaaca tcgtgttggc gttgtgacca ttttttgcaa cacctcccgt ttatgtttca agttgccaga agtgttgggg ttcatgcttg ggaaggtgac caaacttccg tggcagtgac aatggtgatt tctggtztcct atgtaagcga gcatggttcc taatgtaagt aatatggctg Cctcttggcc gtccactatt ggtcatgagt ggtgaccgcg ggctcctaca ttggccaatc ctcgaggaag catggctcac cccatgatgc tgaggacatt ctgctggaat tagctttgga atttgtgtcc agattgttca gtgccatgtg tggaataaac gaaggtgcgc acggtgtgcg aattgaggag gcctggaata cgagatgaag tcctgcagat tgaaccactc <210> 352 <211> 589 <212> DNA <213> Pinus radiata <400> 352 gtctcagggt aacgaaaatg catttg OLt..agccct catgaaggct gtgggtatct ggactttatt gtaaaagagc ggttggcagt gaagtgaaac atcgtgttgg cgttgtgacc gttttttgca acacctcccg tttatgtttc aagttgccag gggaaggtga tcaaactLtLL gtggcagtga caatggtgat atctggttcc aatgtaagcg tgcatggttc ataatgtaag caatatggct gcctcttggc cgtccactat tggtcatgag tggtgaccgc aggctcctac cttggccaat tctcgaggaa gcatggctca ccccacgatg ttgaggacat tctgctggaa gtagctttgg aatttgtgtc cagattgttc ggtgccatgt ctggaataaa cgaaggtgcg tacggtgtgc taattgagga agcctggaat ccgagatgaa atcctgcaga gtgaaccact wo 00/22092 WO 0022092PCT/NZ99/00 169 cagtgttggg gttcatgctt gtcgccgtgc ttctgtaggc cctgagacaa atgtcttggt aatgggggca ggtcctatcg gccttgtcac cgtgctgtct gcacgtgca 540 589 <210> <211> <212> <213> 353 332
DNA
Pinus radiata <400> 353 gacaatatgg ctgcatggct ccgcctcttg gcccccatga gacgtccact atttgaggac attggtcatg agtctgctgg cctggtgacc gcgtagcttt cgaggctcct acaatttgtg cactggaata tgcgaaggtg attacggtgt aataattgag ggagcctgga tcccgagatg aacactcttc cgcatgaagg gcggacttta gaggttggca atatcgtgtt aa gcatccagcc ctgtgggtat t tgtaaaaga gtgaagtgaa ggcgttgtga cttcaaactt ctgtggcagt gccaatggtg acatctggtt ccaatgtaag <210> 354 <211> 312 <212> DNA <213> Pinus radiata <400> 354 gctcactgga ataaacactc ttcgcatcca gatgcgaagg tgcgcatgaa ggctgtgggt acattacggt gtgcggactt tattgtaaaa ggaataattg aggaggttgg cagtgaagtg ttggagcctg gaatatcgtg ttggcgttgt tgtcccgaga tg gcccttcaaa atctgtggca gagccaatgg aaacatctgg gaccaatgta cttcccctct gtgacgtcca tgattggtca ttcctggtga agcgaggctc tggcccccat ctatttgagg tgagtctgct ccgcgtagct ctacaatttg <210> 355 <211> 432 <212> DNA <213> Pinus radiata <400> 355 cttcccccgg cttccaaggt ctctacgacg cagaccctgc attgcccttc ccaatgagga tgggtccaaa agaacgtggt ggcaatgaag tcttcgccgc aatattcaca tggctctcgt cttgccacca gtgtgctcca gtggaaccac ga agtgcagctg agctctccat actctcgaac tgcctacgtt ttctaatgac caaatacaac gaactctttc atcaagagcc gcattcagtg gttgctcgtc ccgggcaccc Ctcacctcct ctcgacggaa ccgccataac aaggcat caa gtaccgacat gtctttcgcg agattacggc accttgtccc ttatcaaggt cggctttcaa caagttgaag taaaatcacc agcctatgcc catagctgtc tgccatgaag gtcgagcccc gagcgacctt 120 180 240 300 360 420 432 <210> <211> 212>~ <213> 356 384 Pinus radiata <400> 356 taag-ccact gaatgggtaa aggcatagct gtagggaacg ggttcccgca atgaaaaaca taaagttacc actccccatt ttcctttgca tcaaacgtga tggttctcct ttcttcataa gatatccctg gcttatgtac atgaaaacat aggtttacac tccattcggc Ctcttgctgt agagcctaat atgcttatcc tatt tcagggcctac aggaactgac ccttgtcagc acttgggaat gaaaccactt atattttgca t Laccaycca acgcagttaa atcggtgcag tcatttccac ttggatttgt tacaagggtg caaagaccac tggcaaacct acatgaatat cgtctgctgg tgtctcagat accccagcca 120 180 240 300 360 384 WO 00/22092 WO 0022092PCT/NZ99/00I 69 <210> <211> <212> <213> 357 420
DNA
Pinus radiata <400> 357 gaccaatcta gctcttcct gatggtgata aaataggagt gaggtggtaa ctttgatgaa gatgttgtac tgcaagcttt gaagaactga agaacatatc attgtgccct tctatcccgc ataggcgatg ccaacaacgt gattggatgt ggactatggc gtccaacaac cgcgaattct ttccagccaa caccaatgtc accctatctt gtggccatct atggacgcaa attgggaaaa ggtatcgatg gactncgcaa aaatacattg gttccggcca tctgttgttc gccatcttcc ctagaattta taatagtggg accgttgggt ctgtgggaaa tgaacaacat agttctcgca atctgcagac ccaggaaaac tgtcgctaac tagcgagcac cgaggttttg tcaaactgcg <210> 358 <211> 399 <212> DNA <213> Pinus radiata <400> 358 ggactacgct ctgtttaggt caacttattc gatgccctcg caacatccca ctcatcgtta ggttgacaac gctcgcgttt gactcccaag aggccgggaa cagaaaagct ggtgatgaga tgtatactct ctaaacttta caacctctac tcgataccct ctgaaagcgg ataacaacaa cgagcattga cggagcgtca ctccgtaact cgtggtgcag tctttctgcc atggccttct tctcatccgc gacctacatc ctttgggctt gcgtcgcag gacgagggtc atggaggact ggtggcaatg catgtgctct ttcgcacttt ttctacctta gcagctacat tggggtatcg at gtggc ca c caaatgtagg tcaacgagaa accaacaatc 120 180 240 300 360 399 <210> 359 <211> 469 <212> DNA <213> Pinus radiata <400> 359 ggactacgct ctgtttaggt caacttattc gatgccctcg caacatccca ctcatcgtta ggttgacaac gctcgcgttt gactcccaag aggccgggaa cagaaaagct ggtgatgaga tgtatactct ctaaacttta atatgaatat gtcctctata caacctctac tcgataccct ctgaaagcgg ataacaacaa cgagcattga cggagcgtca ctccgtaact tgtcaactgc cgtggtgcag tctttctgcc atggccttct tctcatccgc gacctacatc ctttgggctt gcgtcgcagt ctcgatagat gacgagggtc atggaggact ggtggcaatg catgtgctct ttcgcacttt ttctacctta ccgacgaacq at at tat gt gcagctacat tggggtatcg atgtggccac caaatgtagg tcaacgagaa accaacaatc aatagagcca 120 180 240 300 360 420 469 <210> 360 <211> 473 <212> DN'A <213> Pinus radiata <400> 360 gccatgc-aa ctgttgtctg gcaggccttt agggatttgc gctgaaagac ggccttcgct catcggtagc gaagacagct accattttaa gcattctgca tatggacggg aacaacatca gggtcggaga aacccggagg gggctg.,tgt tgtacgttat ttattgttct tggctgacaa gcaaagtgcg tcggactcat ctgccgcagg "ti aatctq ggcttcgttc ttgctctttc tttggcggca gctcttcgac gacagctgtc gtgggtgcag cgatczggatt caacgccagc tatgcagacg ccggccgatg tcagaccctg cccaacgagt ggaaacgt tg 9otactgtaa cttgtacatt ctggaacagt tggtaggcct cggtgcttca tgttggagag tgcccttcca WO 00/22092PCIZ9016 PCTINZ99/00169 cccggcgacc cggatcgaat acatcgcggt gggcaacgag gttttgcaca gca <210> 361 <211> 441 <212> DNA <213> Pinus radiata <400> 361 gtcgccnccc cggtgctgca ttcctggaca tctacccgtt tacgctacgt tgagcctgga tccaacatgc tggacgccca cccagcgtta atgtggtcat ccgggcacca atgtcccgaa gccgacccgc cacgggggac tccctcttta acgaggacca accgttgctc cttcgcctgg ccgcaacacg gctggacgcc tggcgagaca cgccacgctg accccggcgc g gatttcgcga agcgcgaacc gcggaattcc gtgctggcag gggtggccca tacaaccagc cccggngcct accggacggg ccgccaacgt aagacgccgg ccatggaccg caaaaggcga agctcgtaca tcatccccac ctctttcctc gt ci tgga c gctcagctac gctcggattc cgacaatcag aaaggccctc ctatatitc 120 180 240 300 360 420 441 <210> 362 <211> 351 <212> DNA <213> Pinus radiata <400> 362 cgccgtctaa aggagtcttc ttictgcaca gaacggcggt gcaacccaag caacattcat cagacaaaga tcacagctac ccatggaagc ctcggggtat ctgctggcgc ggaagtggcc gtagatgctg cccttcatgg ttggactacg agcaacctgt cccaacatcc accattgaga tgaaggacac cggatgtcta Cccttca tcgatgccat cgatcgtcat atgctcagac gatgggccaa tccatacttc gcccacggct ggttgatact taccgaaagt ctataacaat atactcaaat agctacatcg acgccggtga cttttgtcgg ggatggcctt <210> <211> <212> <213> 363 388
DNA
Pinus radiata <400> 363 cgtgattggg ttgattgccg agtgaactac gggatgcaag gaacggccac aacatcggga ttttgcaaat tctgggatcg ctcctccagc caggactcag caccagtatc aaatatattg cgtatcttgt tccagccatg tcttctgttg gagacaacct aaatgaagct atgtaatcgt caaatggttg cggtgggcaa aacaacat tgcaatcgtc gccacctgca tttcaatcca aggagtcagt ggttaatgac cgaggttttg actgatggtg gaccaggtgg gacggtggtg aacaacgatt aatattgtgc cctagcacgc ataaaatagg taactttgct cattgaatgc tgcaggccat gctattccag agtaccgtat <210> <211> -,212> <213> 364 560
DNAJ
Pinus radiata <400> 364 caccacaaca ggaatcaact agtgggtctt ttgcagacca agtcctgaaa gcatttgcaa cattggcaac ttgacagact ttacttacca gccacaaaga tgcacacaag ttaatggcaa ctggactggc atggacaagt gcaatatcgg acactggtat caaactcagc tcataggtat acctagttcc LCLttcaaat tgcagacgac cagaataaaa agaattgatt cactgaatgg agcagtaggg tgcaatgcaa gccctgtcca ttgccaccac ctctacactg gttggagtgg gtcaatgaaa aacgaggttt aacattcatt gcacaagtgc cggaactagt taaatgcgac caaatgatat acattcaaac acacaggaac cggccctagt WO 00/22092PC/Z/016 PCT/NZ99/00169 cagcattggt gcggacacgg atattataat tagcactccc cattctcttg gtgtacttgc gacttcatat ccaccatctg ctggttcatt tcaaccagga ctggagagcc tactggaaca gcttttggct ctcctgtctc <210> <211> <212> <213> 365 494
DNA
Pinus radiata <400> 365 ggagtttggc gcagcgttta ttcctccacc atctgcagta tatatggagc agatcctgca taggcattgg taacgatcaa ttaagaacaa tatcgttcct acgaggttct gttcagtggg acctacacac tgcccttgtt attctctggc catactttct ttgacatgaa atcc ccagccctag g caa agc tgg attcttcaag atcccatctc tttgttcctg gatgggagtc gaggtttcac acatctgtcc gcgttgacta ttcagagtac catttgctaa tgaaccagct ccactgatat tgatttccca ttgaccagca ccccatctgc tggacaaact aagtatttca cacaggaatt ggctgttgca cattggaatc gctcctccct aattaaggtc tggccgtttc gcagacaatc aagttgagac gggttagttg cagaattgga tcggtgggga gcattgcaga tccacacctc aatgaaagtt <210> 366 <211> 365 <212> DNA <213> Eucalyptus grandis <400> 366 acgaattgga atgataatga ggcgaatacc agataacaaa cattggaata attacattac gccgtgagga ttccggtagg gtgggaggct cgctgtatgc aaggtaatag ttgatctcca acqaq cccgtccatc tggctatggc cgagcaggac atggtggatt act gga cat g tgctgctcca ttcgatataa cccagtactg ttccgattta gcatatgatc gcttttacat gggtcgcaaa acattgttgg ccccacaact tggcgaccaa caacaccgcc gggcagagaa atgccgaatc tggcttacaa cttacgggat taatgtaact gaagcctttt ttatcgtatg ttatagtgcg <210> 367 <211> 435 <212> DNA <213> Pinus radiata <400> 367 cagcggcggg ccttaatcat gcggagaacc gttccatcaa agaagcacaa tatcaaggtt ttgacaattc cggcctccta cccgaggcag caacatcatc tgactgcaat tgctcctcta ctgcgaacaa cattggctcg caaagccaca tgctt gttcgtatcc ggacaggctg atcgtcgacc acatcgacac aagaaattag aatgagccag nagttatggg ccatcgggta attatttggt tgcatggtgt cctcatggga ccgcccaatt caggatatct agtatccgca ttgggcttac taaggctatt cccaggcagt cacaaacagc tgccatgcag tagcccacgt ctccatttgg gatgtttcgg ggatgggcac cagaacggtt acaaatattg accaacgtcg ctcctcgata caattcaacg <210> <211> <212> <213> 368 630
DNA
Pinus radiata <400> 368 ctttcatcac agaggagatt tcgttttcat gtccaagaac gggataagtg ctgtcagaat tccagttgga tggtggattg ctagtgatcc atatcctcct gctccttttg ttggaggatc WO 00/22092 WO 0022092PCT/NZ99/001 69 tctagcttgt cgatctccat atttatagaa tcttgctgcg acggtctcct agttcgaaag tcccaaggag ttacaatctc catcaaaacc ctcgacaaag gctgctccgg tggcctgagt agatatgctg gcagtaagtt tattcgtcgt ttgtttcaaa tacgatgacg acaagagctc Ctttctcgtg gctcacaaaa cgcaagagaa cacaccctgc tgaacaatgt cggcgtatgt tgaacaacgg ccactttcaa aaactctgca ggcacagaac cggtgatgag catcgacaag ccttttgggc gactgattac gataatgtgc cttatcccgc aaatatgacg catgacatta cacagtggca agtctatctg attgaactat tattcacggg aacagaatcg acagttgtgg gttcagcaga aggtgattat ccagagatgg tcattgattt tgaatgaacc gttacgacat gccctgctga atgtgcatta acatcgacta 180 240 300 360 420 480 540 600 630 <210> 369 <211> 507 <212> DNA <213> Pinus radiata <400> 369 aacggccttg acggagagtg ggatggtgga gctctggata catgctgctc gagtggcctg ggcaggtatg cccggtgtac aagcattcgt gacctgacaa actttgcatt ttgccagtga acgcattcac cgggttccca attcacaaga cttcaaactc cggtgaacac cctctgccta agctcctcaa catgtccagt ccccaatcct atgggctaag aaacggagat caacatcgat agctctcctg attgaaaaca tgtgata gtcatgaatg aatggtataa CCtgctccgt aatcataaca gcacacagcg aaaagtatat ggaattgagt tattataaaa atcactggaa atgctgtgag ttgtaggagg taggggtaat gcacgagaga cagtaatcga tgctgaatga ggggttatga cagcttcatt aattccagtt atctctgaaa tgtggatctc tggatatctt ctttctcgct accccgagca caccgttcga 120 180 240 300 360 420 480 507 <210> 370 <211> 480 <212> DNA <213> Pinus radiata <400> 370 caatagtgtg catatcaaag gctgactgcc gatttcgaag gatgaccatc gtaaggacgt gaaagctaca caagtcttga tttcatgtcc aagaacggga tgatccatat cctcctgctc ctcgtgggca cagaaccat9 acaaaacggt gatgagcaca tcttcaatgg gggagcctgg tgcaggggga acgaacatcg taagtgctgt Cttttgttgg acattaaggt gtggcaccag aatgtacatg ttgggacgac gtttcaaatc aagcactttc cagaattcca aggatctcta gattatcgat agatggattt caggcacaat aataatgctg tcaaacggtt atcacagagg gt tggatggt gcttgtctcg ctccatgctg atagaatggc ctaaagatca caactttcga atggacccga atgatttcgt ggattgctag acaaagcttt ctccgggctc ctgagtcgca 120 180 240 300 360 420 480 <210> 371 <211> 366 <212> DNA <213> Pinus radiata <400.> 371 gatcaattga ctgccgactt ttcgagatga acgttgtaac ggccttggac ctgacaaagc gagagtgact ttgcattcat tggtggattg ccagtgaccc ctggataacg cattcacatg gctgct ccaaggtaag tggagataat tcctcaagtc gtccagaa caatcctcct ggctaagaat cctggatgga ggcataggag atgaatgatc qgtat.aty gctccgtttg cataacatag atgatggcaa gggagtacca actggaacag ctgtgagaar taggaggatc gggtaat tgt tgccgcaaca gctaacaaac cttcattacg tccagttgga tctgaaagct ggatctccat 120 180 2 4 0 300 360 366 <210> 372 <211> 427 WO 00/22092 WO 0022092PCT/NZ99/001 69 <212> DNA <213> Pinus radiata <400> 372 ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt acgttgcaga ctattggcat aggcataaac ggtgtacggc ggcgggcatt cccccgattt caccatcccc tgctctgcca 120 gccattcacg catgtaatgg cccatataca Cttcgagcac gccacctttg gtggtgagtt 180 gcccaccggg gacgtcccat tctggccatt tattcggcgt cgactgctcc agcacactgg 240 ccattgttcg ccagcggngc acgtaagtta tggcggctca tcatgagcac ttgctgtagc 300 tgatagcctt ccggtacgnt tgtgcctgag cggtttgaag cgagttaaac tatccctgcc 360 acagctgcgg gattagcgtt ttgttcaatc ctggcacctc ttttggttat caatcactaa 420 gagtgtg 427 <210> 373 <211> 384 <212> DNA <213> Eucalyptus grandis <400> 373 atgaatatta tctgcacgtt atgaatatta tcgcggttcc agnggggcaa aaagaatcga ttctttttgc ccctctgaat actatctgca cgttatgaat attttggaac tgatggtcgt 120 ggtggatatt ttgatgaata cgggatcatc cgtgatatta ttcaaaacca tcntttgcag 180 gttttctgtc tggttgccat ggaaaanccc gtatctctca aaccagagca caattcgcga 240 tgagaaagtg aaggtccttc aatcagtact tccgattaca gatgaagagg ttgttcttgg 300 acaatatgaa gnttnagggn cgatccaatg tccctgacaa ttcaaatact cccacttttg 360 caactatggt tctacgtata cata 384 <210> 374 <211> 368 <212> DNA <213> Eucalyptus grandis <400> 374 ggcagggaaa gctctaaact caagaaaagc agagatacgt gtccaattta aggatgttcc tggggatata ttcaaatgta agaagcaagg gagaaatgag ttcgtaattc gcctacaacc 120 ttctgaagcc atgtacatga aactcacggt caagcagcct gggttggata tgtcaaccgt 180 gcagagtgaa cttgatttgt catatcggca acgttatcaa ggagtcgtaa ttcctgaggc 240 atatgagcgt cttatacttg acacgtacat ttctttcctc tttatttaga gtacttccaa 300 cttaaaaaaa tctcgtagct ctttgtttgg agcttagctc agtggagtag acttgcattc 360 aggcgggc 368 <210> 375 <211> 161 <212> DNA <213> Eucalyptus grandis ggcagggaaa gctctaaact caagaaaagc agagatacgt gtccaattta aggatgttcc tggggatata ttcaaatgta agaagcaagg gagaaatgag ttcgtaattc gcctacaacc 120 ttctgaagcc atgtacatga aactcacggt caagcagcct g 161 <210> 376 <211> 283 <212> DNA <213> Eucalyptus grandis <400> 376 WO 00/22092 WO 0022092PCTINZ99/00 169 gtgcagatag tattcagaga agattttgga tacgggatca tccgtgatat tattcaaaac atggaaaaac ctgzatctct caaaccagag caatcantac ttccgattac agatgaagag gacgatccaa ctgtccctga caattcaaat actgatggtc catcttttgc cacattcgcg gttgttcttg actcccactt gtggtggata tttggatgaa aggttttctg tctggttgcc argagaaagn gaaggtcctt gacaatatga aggttacagg ttg 120 180 240 283 <210> 377 <211> 363 <212> DNA <213> Eucalyptus grandis <400> 377 cacggtcaag tcggcaacgt aatcaggggc ttttacaccg agggagccga gcatggttac ttc cagcctgggt tatcaaggag gaccaacagc atgcttcaca ggtcctgttg atttggatcc tggatatgtc tcgtaattcc actttgttcg gaattgacga aagcggacga ctccaacctt aaccgtgcag tgaggcatat gagagatgaa tggtgaattt gctgctggaa gtagatatga agtgaacttg gagcgtctta cttaaggcgg aagccaattc aaagctggtt agacaccgcc atttgtcata tacttgacac cttgggagat cataccaacc acgttcaaac acaaataaat 120 180 240 300 360 363 <210> 378 <211> 457 <212> DNA <213> Pinus radiata <400> 378 gtcgcttaaa gatgttgtgg tgcacagtgc tcttcaaaca gatttctagc aaatgttgat aaactacatt agttgttgtg ctcgaacatt aaggacatgg tggtggcagt tagtactaac cttttgcatt ctgggattgg tccccttatc acttcagtat cagtcaggta gatcctgagg ccagttgatg gtatcaaaga atcacatctg cttaagcttg gttggcggtc gggtttccta ttggtggcag cagctcaatg tcgctcggag cattcacaac cccttggttc taaaagaatt gctacagcgt ttgttag cttitctgggt tgcaaatggg tattgatggg agcagaaacc tgaagctgtt tggaatagac gtgcagtgct ccactttttg cgtcaactgc ttaaacccgg atgctaaatg gcaaagcaca ccacaaaatg gtgggtgccc <210> 379 <211> 386 <212> DNA <213> Pinus radiata <400> 379 gtcgctcgga gtattgatgg acattcacaa cagcagaaac gcccttggtt ctgaagctgt gtaaaagaat ttggaataga cgctacagcg tgtgcagtgc attgttagca agtttctgga tttgagaaaa atattcctgt gttaaacccg catgctaaat tgcaaagcac cccacaaaat tgtgggtgcc gggagcaaga tttgct gnaactacat gctcgaacat atggtggcag gcttttgcat ctccccttat agtatagata tagttgttgt taaggacatq ttagtactaa tctgggattg cacttcagta accatttcca ggtatcaaag gatcacatct ccttaagctt ggttggcggt t ggg tt tcc t cacaactcca <210> 380 <211> 365 <212> DNA <213> Pinus radiata <400> 380 tgcagtgctg tgggtgccct ccccttatca cttcaatat9 ggtttcctat tgttagcaag tttctggagg gagcaagaag taiagataac catttccaca caactccatt tgagaaaaat WO 00/22092 WO 0022092PCT/NZ99/001 69 attcctgttt tgcttggtct cctcagtgtg tggaatgttt cctttcttgg gtatccagct agggctatct tgccatacac tcaggctctq gagaagtttg caccgcacat ccagcagctt agcatggaga gtaatgggaa gggagtttct attgatggtg tgcccctcaa ttttgaggct ggtgaaatag attttggaga acctggtaca aatggccagc atagctttta ccagttaatt catca <210> 381 <211> 491 <212> DNA <213> Pinus radiata 180 240 300 360 365 <400> 381 tgcagtgctg tgggtgccct tttctggagg gagcaagaag attcctgttt tgcttggtct agggctatct tgccatacac agcatggaga gtaatgggaa ggtgaaatag attttgyaga catcagggtc gtgtaattcc tatttacaag gggaagttgt ccggatgctc t ccccttatca tatagataac cctcagtgtg tcaggctctg gggagtttct acctggtaca ttgtgatttt gagtaaccac cttcaatatg catttccaca tggaatgtt t gagaagtttg attgatggtg aatggccagc attggcatcg gatgagctta ggtttcctat caactccatt cctttcttgg caccgcacat tgcccctcaa atagctttta tcaagagcca tgtctaactt tgttagcaag tgagaaaaat gtatccagct ccagcagctt ttttgaggct ccagttaatt gcagcccatt ttttgcacag <210> 382 <211> 446 <212> DNA <213> Eucalyptus grandis <400> 382 tatgcgcaga ggcaaaatca gtagtgaaaa gtactctcca atccctcaag gcccacagtc tttccacaat ccaatcctga.
ttattaactc ccatccaaaa tcttccttgt taggataacc agaaaaacga atcatttgac actgagtctt gccaccatat atgttcggta gggttcacgg agcagatggt agagtatcac atggtgggat ttgacataat gagcttaaac gcttcc tctcagaatt ttggcttttt tcttgtcaat ccgaattatc cattcctata ctttgaagta caatgccttc cctaatgaaa cggactattg tgattttaca tgtcatgcaa ttcaacgcca ctgccgaata ttcccacgcc tcagatccaa ggaccataaa tatgctgaca cgtggctcat gtaatggtgt gaaggatcgt cacttgacgt <210> <211> <212> <213> 383 464
DNA
Eucalyptus grandis <400> 383 tatgcgcaga ggcaaaatca gtagtgaaaa gtactctcca atccctcaag gcccacagtc tttccacaat ccaatcctga ttattaactc ccatccaaaa tcttccttgt taggataacc agaaaaacga atcatttgac actgagtctt gccaccatat atgttcggta gggttcacgg agcagatggt agagtatcac atggngggat ttgacataat gagcttaaac gcttccaagt tctcagaatt ttggcttttt tcttgtcaat ccgaattatc cattcctata ctttgaagta caatgccttc tattaactaa cctaatgaaa cggactattg tgattttaca tgtcatgcaa ttcaacgcca ctgccgaata ttcccacgcc gcta tcagatccaa ggaccataaa tatgctgaca cgtggctcat gtaatggtgt gaaggatcgt cacttgacgt <210> 384 <211> 385 <212> DNA <213> Eucalyptus grandis WO 00/22092 PTN9/06 PCT/NZ99/00169 <400> 384 tatgcgcaga ggcaaaatca gtagtgaaaa gtactctcca atccctcaag gcccacagtc tttccacaat ccaatcctga ttattaactc ccatccaaaa tcttccttgn taggataacc agaaaaacga atcatttgac atgttcggta gggttcacgg agcagatggt agagtatcac atggtgggat ttgacataat gagct tetcagaatt t tggc tt t tt tcttgtcaat ccgaattatc cattcctata ctttgaagta cctaatgaaa cggactattg tgattttaca tgtcatgcaa ttcaacgcca ctgccgaata tcagatccaa ggaccataaa tatgctgaca cgtggctcat gtaatggtgt gaaggatcgt 120 180 240 300 360 385 <210> 385 <211> 433 <212> DNA <213> Eucalyptus grandis <400> 385 tatgcgcaga ggcaaaatca gtagtgaaaa gtactctcca atccctcaag gcccacagtc tttccacaat ccaatcctga ttattaactc ccatccaaaa tcttccttgt taggataacc tagaaaaacg aatcatttga gtactgagtc ttg atgttcggta gggttcacgg agcagatggt agagtatcac atggtgggat ttgacataat cgagcttaaa tctcagaatt ttggcttttt tcttgtcaat ccgaattatc cat tcctata ctttgaagta ccaatgcctt cctaatgaaa cggactattg tgattttaca tgtcatgcaa ttcaacgcca ctgccgaata cttcccacgc tcagatccaa ggaccataaa tatgctgaca cgtggctcat gtaatggtgt gaagggatcg ccacttggac <210> <211> <212> <213> 386 349
DNA
Pinus radiata <400> 386 gggtttgtca ctactagcgg ggcgcaaatt catattggct cagcagatgg aggacgctgg gcgactgaat taccgacggc ggcaccttca ctctcaatga gcagcggagc gacacaatat caccgagttt cccacttctg tatcaaggtt gctcgccacc aggtccgcag caagatgatc gagttggacg acggatcctg cttcgtactt aaccttacct ggtcttcagc atcgcgttca gnaagccatt atnacgtcga ggggcttcaa actaccaggt gcctcgatgc cgaacaact tgcgtttgtt gtcgacgttc tgctattaat ctgggatgag agtcgt cgcg <210> 387 <211> 438 <212> DNA <213> Pinus radiata <400> 387 ctggccctcg tagaatcctg tttcttcagt attttgtttg aatctgggca tcctcatttg aaaatcaatt cctgtgattt attgttccct ttgttggact tccatcgatq gatttgacat cgaaggatca ctttgacaac atccttgnag gctactct ggtccttgga catcttccaa atcctgccaa ggttattggt ctccataaaa gactagccat gaggctcgtt tgatttccca atgggttgat ccagatatca gatgaaattt cccttccaag ttcctctatc catcaactcc aactctgcaa atccaatcat gggtaggaat gtccccacag ccaacctcga catccctgta catgcaaaga acagaactgg gtagaaatga gaacagttgc agaagccggg ggagatgact tggtagcccc ttgtagggtc <210> 388 <211> 414 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 388 ggggtttgtc actactagcg tggcgcaaat tcatattggc ccagcagatg gaggacgctg tgcgactgaa ttaccgacgg gggcaccttc actctcaatg ggcagcggag cgacacaata cggaggattg gatctctatg gcaccgagtt tcccacttct gtatcaaggt cgctcgccac aaggtccgca tcaagatgat t tcgc tgga t tgagttggac gacggatcct tcttcgtact caaccttacc gggtcttcag catcgcgttc cattcctggg gggaagcca t gatgacgtcg tggggcttca tactaccagg cgcctcgatg acgaacaact agtacgcacg ttgcgtttgt agtcgacgtt atgctattaa tctgggatga cagtcgtcgc gggtgggata acga 120 180 240 300 360 414 <210> 389 <211> 625 <212> DNA <213> Pinus radiata <400> 389 gttgactaga atgggagctc atggatagag tggtttggaa tacagatttc tcctgacatt gataaagaac gtttgggaaa tatggtttat gttttggcag gtctgagaat gtgaacagca atgaacgagc gagatggcta ggtttttatg atcaccaata tggcttgctg catctgcaag tcttacaaaa aaacatgtat ctgatggcag ccatccacag ttactggagt ct cgt tgt ca gccaggtcaa gagaatacaa accaaatcag gggcagatga atggaagcag gcccaggatt atgcatctgc aangaatgag cagct agcctacaag aagtgatcct atccattgat aaaagagatc aggact tga t ggagacccag gatattgaaa tcacatgagt taaatgggga ctcatatgga gatgacccta tctgggagga ggaaaccatc aatccaggct tttgcaacag ctttcatttt aaaccagttt gagagagaca ggagcaggtg gatgggtatg caatctttgc ccatccaggg ttctggaggc tctctgtggg ttcattccta tgcagagttg tgtttgcaga atttacttga gaggggccat agattgtttt 120 180 240 300 360 420 480 540 600 625 <210> 390 <211> 78 <212> DNA <213> Eucalyptus grandis <400> 390 caccaccggc aggtgatcca gagacggatt ttcggcccat gcttcggtca gcgccggacg cagttggttc tggctggc <210> 391 <211> 178 <212> DNA <213> Eucalyptus grandis <400> 391 acccctttca ggcgttggtt agcgtteggt gtgcggcggc cgttaaacca gtcgagcacc accggcaggt gatccagaga cggatttttg gcccatgctt cggtcagcgc cggaagcagt tgtttctggc tggcgttgat ttgcgttttc agttccggat gctgggcagg caagctgt <210> 392 <211> 359 <212> DNA <213> Eucalyptus grandis <400> 392 accccttica ggcgttggtt agcgttcggt accggcaggt gatccagaga cggatttttg tgtttctggc tggcgttgat ttgcgttttc agcggccagc cgagtacgcg accaaaccag gcttncagac cgataaatcc aggcaccacg gtgcggcggc gcccatgctt agttccggat gcgtagatat ctgctggcac cgttaaacca cggtcagcgc g-ctgggcggc caccaaacgc cacacccagg atcgagcacc cggaagcagt aagctgttcc cgattggcct atgtcggcaa WO 00/22092 WO 0022092PCTINZ99/00 169 ggtggcggaa cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtg 'z210> 393 <211> 510 <212> DNA <213> Eucalyptus grandis <400> 393 acccctttca accggcaggt tgtttctggc agcggccagc gcttccagac ggtggcggaa aaaacgtaag ctggtggtaa tgctctgggg ggcgttggtt gatccagaga tggcgttgat cgagtacgcg cgataaatcc cacgcagaaa tttgcctgat gt cgtgctga gatacggtcc agcgttcggt cggatttttg ttgcgttttc accaaaccag aggcaccacg tctgcggccg gagtcaatcg ttatcgatct cgctgttcc gtgcggcggc gcccatgctt agttccggat gcgtagatat ctgctggcac ctgtgtcagc atctgttcaa gggcagcaaa cgttaaacca cggtcagcgc gctgggcggc caccaaacgc tacacccagg aacgtcgccg cagggctacg tacctgatcc gtcgagcacc cggaagcagt aagctgttcc cgattggcct atgtcggcaa gtgacggtga ctggctgtgg tccagaactt 120 180 240 300 360 420 480 510 <210> <211> <212> <213> 394 469
DNA
Pinus radiata <400> 394 gaggtacgcg gttatagcat catcaggttg tcggttaact tttatcgaca atccattcgc cgaagccgaa ggcggtacga tacccacgct ttggcgtctt accgcgtacc atgttttcac acgtttcacc tgccctttgg ggcggtagtg actaaatcaa gacccgtgtt ccatgcctgg tgaaggtttc tgcggtcaac ccggcagggc aggcgatgat cgattgccgg cctgagcaat cagggtgaag gatgttcggc taccgtcact ggcggaatcg gttcatcaca gttcagcggg agcaatacgt cagatcaacg agtttacgtt agtgcgcctt tccagcggat acaaagccaa tggcctttca gattcattac tccagcacaa acatcatca cgacaaatgc tgaactgcgt cgttagttgc cgtgttcttc gctgcgtggt cttgttcttt ccgggccaac 120 180 240 300 360 420 469 <210> 395 <211> 443 <212> DNA <213> Pinus radiata <400> 395 aatcgcgtca cgaatggtct acccgtgttc agggtgaaga catgcctggg atgttcggca gaaggtttct accgtcactt gcggtcaacg gcggaatcga cggcagggcg ttcatcacat ggcgatgatg ttcagcgggg gattgccgga gcaacacgtt gatgaccggc gtttacgttc gtgcgccttt ccagcggatc caaagccaac ggcctttcag attcattacc cca cagttttccg gacaaatgcc gaactgcgtt gttagttgcc gtgttcttct ctgcgtggta ttgttcttta aggtacgcgg atcaggttgt ttatcgacaa gaagccgaag acccacgctt ccgcgtacca cgtttcacct ttatagcatg cggttaactc tccattcgct gcggtacgat tggcgtcttc tgttttcaca gccctttggc 120 180 240 300 360 420 443 <210> <211> <212>. <213> 396 252
DNA
Pinus radiata <400> 396 tctcgtcgag aatcgcgtca cgaatggtct gatgaccggc cagttttccg aggtacgcgg ttatagcatg acccgtgttc agggtgaaga gtttacgttc gacaaatgcc atcaggttgt cggttaactc catgcctggg atgtccggca gtgcgccttt gaactgcgtt ttatcgacaa 120 180 WO 00/22092 WO 0022092PCT/NZ99/00I 69 tccattcgct gaaggtttct accgtcactt ccagcggatc gttagttgcc gaagccgaag gcggtacgat gc <210> 397 <211> 395 <212> DNA <213> Pinus radiata 240 252 <400> 397 aatcgcgtca cgaatggtct acccgtgttc agggtgaaga catgcctggg atgttcggca gaaggtttct accgtcactt gcggtcaacg gcggaatcga cggcagggcg ttcatcacat aggcgatgat gttcagcggg gatgaccggc gtttacgttc gtgcgccttt ccagcggatc caaagccaac ggcctttcag gattcattac cagttttccg gacaaatgcc gaactgcgtt gttagttgcc gtgttcttct ctgcgtggta cttgt aggtacgcgg atcaggttgt ttatcgacaa gaagccgaag acccacgctt cccgcgtacc ttatagcatg cggttaactc tccattcgct gcggtacgat tggcgtcttc atgttttcac 120 180 240 300 360 395 <210> <211> <212> <213> 398 422
DNA
Pinus radiata <400> 398 tctcgtcgag aatcgcgtca ttatagcatg acccgtgttc cggttaactc catgcctggg tccattcgct gaaggtttct gcggtacgat gcggtcaacg tggcgtcttc cggcagggcg atgttttcac aggcgatgat tg cgaatggtct agggtgaaga atgttcggca accgtcact t gcggaatcga ttcatcacat gttcagcggg gatgaccggc gtttacgttc gtgcgccttt ccagcggatc caaagccaac ggcctttcag gattcattac cagttttccg gacaaatgcc gaactgngtt gttagttgcc gtgttcttct ctgcgtggta cttgttcttt aggtacgcgg atcaggttgt ttatcgacaa gaagccgaag acccacgctt cccgcgtacc acgtttcacc 120 180 240 300 360 420 422 <210> 399 <211> 305 <212> DNA <213> Eucalyptus grandis <400> 399 ccgataagaa gctcatcccc gncaagtcca agagttctcg aggaccatca tcatggagtc ctttatgcca tgggagggtg atttcgcgct caaggggccc ggtgcgaaca tcaacgcccg taagcaggaa gcagccaagt tcaccgctgg agcct agttccagac gtcaatcgag c aana ccct g agtgaagtgg gacttttctg ctacctggga gacttcattg tactatgcgg cccggttaca gatggggatt cggnc cigga atccggcagg agtttaacaa agaagatcaa ggatcaaggg 120 180 240 300 305 <210> 400 <211> 372 <212> DNA <213> Eucalyptus grandis <400> 400 atcgtctaca acagcaacta gtcctcggtg agcagttcct aagcaccagg ccgtggcgct atggacgggt accaggacac accatctcgg gcaccatcga aagatgctcg tccgcaagcc cgtcgacggc cgccaaggac gagggt ccag cctctacacc cttcatcttc gctggacaac gtcaacacct atcgggttcg tcggacttct cacgcccacc ggcgacgcct cagcagtgca tcaagaccgc agaacgacgc ccgtcttcta gccagttcta ccgccatctt tcgtcaccgc caccgtcgcc aggcgccatc caactgccac ccgcgactgc ccagaactgc ccagggncgg 120 180 240 300 360 WO 00/22092PC/Z9OI6 PCT/NZ99/00169 aaggagaggc gc <210> 401 <211> 262 <212> DNA <213> Eucalyptus grandis <400> 401 gttcttccgg gagtgcgaca tctacgggac cgtgctccag aactgcagcc tctatgcccg cacggcccag aaccgcaagg acccgaacca gatcctggcc acgtcggacc tcgccgcctc gccctggaag ttgtactcga gg tgtcgacttc atcttcggga acgccgccgt caagcccatg cccatgcaga agaacaccat gaacacgggc atctcgattc acgcgtgccg aaacgggagc ttcctctcgt acctcggccg <210> 402 <211> 403 <212> DNA <213> Eucalyptus grandis <400> 402 gcccgacgtg gttgtggccc gaacgaggtt cctaagaaga caaagagaaa gtactggtag gacgaagact gtgatcaccg cgcaacattc actgccatag ggcaggcgcc gagaagcacc ttacaactgc cacatggacg aggacggaag acaacaagac acaagaagac ggagcttgaa gaagctactt aggccgtggc ccttccagga cggcaagtac cttcgtcata gaccaatgtc ctatgtcgat catagccaag gctacgcgtc caccctctac aagaccatcc tacatcaagg atgattatcg ggagtccaaa gacatcaagt cagtccgaca 9c9 gtgaggctct aaggagtgta gcgacggccc cctgcaacac ttgagaactc tgtccgtaat 120 180 240 300 360 403 <210> 403 <211> 408 <212> DNA <213> Eucalyptus grandis <400> 403 gcaaggaccc caaccagaac cagatctcga ggcatctaaa actcgagggt tgtgtacatg ggagtggaac ggagactttg gcccggggca gccgtcggcc aacagaggca aacaaattca caccggggtg gcattcgtgg accggcattt ggaagcatcc ttgtcctaca cgctagacac tacgtgtgaa cagtcgcgca c tgga ctat c cgatccatgc cgacgtacct tgggcgat ca tttgtattac atggccgggt gttcatattt aacttgaaag ttgccagatc cgggcggcca cattcacccc ggagagtaca ttccgagtca ggatc t tcat ttgaaagt gtcgccgctc tggaagatgt gaaggtggct tgaacgatgg tcacatccac ggttgccgtc 120 180 240 300 360 408 <210> 404 <211> 361 <212> DNA <213> Eucalyptus grandis <400> 404 gcggcggcgc agtatccaga gcggcggagg tacaggcgga acgattgcgg aggcgataaa gtgcgagccg ggaggtacga atgttcatcg gcgatgggaa aagatgacca cgttccacac a gtggctaggg catagtggtg gaaggcgccg ggaggataac gggcagaacq cgcctccttc gagagagaga gcgaaggacg gagagcagtg ttgaaggtgg gtcataacgg gcgggcgagc gggagctgct gagcgagcgg gccggaggat ggaagaagaa gcggcaaaag ggaccggttt ggacatgccg gacgtacaag catcatctac gacgaacctc tgtagccgac cattgcccgc <210> 405 WO 00/22092 WO 0022092PCT/NZ99/0O 169 <211> 227 <212> DNA <213> Eucalyptus grandis <400> 405 ctacaatcat caccggtgat gatagtgtgg ctgggggttc ttccttgcgc ggctctgcta cattcacaat caccggcgat ggatttatag cccgagacat cgggttccaa aacacagcgg ggccccaagg tcaacaggct gtcgccctaa ccgtggcctc cgatcacgcg gcattctata ggtgcagcat cgcgggctac caggacacat tgtacgcgct cgtactc 120 180 227 <210> <211> <212> <213> 406 373
DNA
Eucalyptus grandis <400> 406 tttttttttt aatctccaaa tcccccagaa agaaaggacc gtggacttca ccaagtccct ctgggctggt gatgacatga gtcccttgtt catgtactcc ccgaccaccc tgtgggatca agtacaactt cca cattccaaat aatactcatt ccctgaatca taacctggcc ccgtagtaca atgtgatcac tattgaattc tttcacagcc gctcggccac acttcacccg gagtcttgag caatgtttga acaacacaac cttcagtgta ggtgaacttc cttgctcgtt ggcatgatcg ttgcatcacc ttacagaaac agacacicca ttcgcctctg cccgcgcctg ccgctccaca acagtcctag <210> 407 <211> 190 <212> DNA <213> Eucalyptus grandis <400> 407 cccaaccaga acaccggcat ttcgatccat gaggcatcta aaggaagcat cccgacgtac gttgtgtaca tgttgtccta catgggcgat aacggagact gcttgccaga tcgtcgccgc tccagatctc ctcgggcggc catggaagat gtactcgagg cacattcacc ccgaagggtg gctggagtgg 120 180 190 <210> <211> <212> <213> 408 387
DNA
Eucalyptus grandis <400> 408 cccaaccaga acaccggcat gaggcatcta aaggaagcat gttgtgtaca tgttgtccta aacggagact ttgcgctaga gcagccgtcg gcctacgtgt gcaaacaaat tcacagtcgc gtggcattcg tggctggact ttcgatccat cccgacgtac catgggcgat cactttgtat gaaatggcct gcagttcata atcaact gcttgccaga ctcgggcggc cacattcacc tacggagagt ggtttccgag tttggatctt tcgtcgccgc catggaagat ccgaagggtg acatgaacga tcatcacatc catggttgcc tccagatctc gtactcgagg gctggagtgg tgggcctggg cacaacagag gtccaccggg <210> 409 <211> 482 <212> DNA <213> Eucalyptus grandis <400> 409 gccgtcgggc ctggggttcg acggcggggt cccttcgggg ctgacggcgg acgcgacggt gtgcaaggac gggggaagcg ggtgctacgc gacggtgcag gccgccgtcg acgccgcgcc WO 00/22092 WO 0022092PCT/NZ99/00 169 ggagaacgtc aacggtgagg aaccgtcatc caccgtcgga aggtccggtc gaactgcgag ca qgcggaggga gtcccgttcg accgggtcct gtgctcggcg acccaccagg ttcttgggga agaggi tcgt agaagaagaa ccaacgcggg acggattcat cggtggcgtt accaagacac gatccacatc cgtggtgttc gcaacctggg ggcaagcggg ccggtcggac gctctacgcc aaggaagggg ctgggggacg gtctccacct ctcacgatcc agcgatttct cactccctcc tgtacgagga gcatgggcaa acaacaccgc agaacaccgc cggtcatcga ggcagtacta 180 240 300 360 420 480 482 <210> 410 <211> 424 <212> DNA <213> Eucalyptus grandis <400> 410 cccaaccaga acaccggcat gaggcatcta aaggaagcat gttgtgtaca tgttgtccta aacggagact ttgcgctaga gcagccgtcg gcctacgtgt gcaaacaaat tcacagtcgc gtggcattcg tggctggact tgt t ttcgatccat cccgacgtac catgggcgat cactttgtat gaaatggcct gcagttcata atcaacttga gcttgccaga ctcgggcggc cacattcacc tacggagagt ggtttccgag tttggatctt aagttgaaag tcgtcgccgc catggaagat ccgaagggtg acatgaacga tcatcacatc catggttgcc tgacatttac tccagatctc gtactcgagg gctggagtgg tgggcctgg cacaacagag gtccaccggg agatacgata <210> 411 <211> 519 <212> DNA <213> Eucalyptus grandis <400> 411 gaaggacggt gagcagtggc gaaggtgggg cataacgggc ggcgagcgga gaagcaccag catcgttggc cgacatctac gcaacatcta ncgaacgggt cggaggatca aagaagaaga ggcaaaagtg gccggtttca gcggtggctc tatcaggaca gggaccgttg tgcccggaag cgtacaagac tcatctacgt cgaacctcat tagccgacaa ttgcccgcga tccgggtagg cgctctacgt acttcatctt ccc at ggcc a gattgcggag gcgagccggg gttcatcggc gatgaccacg catgaccttc agctgaccat ccactcgaat tggcaacgca agcaaaaga gcgataaaga aggtacgagg gatgggaagg ttccacaccg gagaactacg ggcgtggtct cgccagttct gcccgtggtc aggcgccgga aggataactt gcagaacggt cctccttcgc ccgggccgga ataggtgcag tccgtgaatg atncaaaaga 120 180 240 300 360 420 480 519 <210> 412 <211> 395 <212> DNA <213> Eucalyptus grandis <400> 412 ccttcatcca ttctccactc agctcgggcc ctcctccgat taaagaaggc gccggagagc acgaggagga taacttgaag ggaagggcag aacggtcata acacgccc ctt~cgcgcg actacgccgg gccggagaag ccctgccccc ccccagctcg agtggccgga gtggggaaga acgggcggca agcggagccg caccaggcgg acacagtaca aggatgccct ggatcatcat agaagacgaa aaagtgtagc gtttcattgc t ggc t agaaatgggt cccttcccca ctacgtgcga cctcatgttc cgacaagatg ccgcgacatg cgcggcggca ccggaggcga accgggaggt atcggcgatg accacgttcc acct-tcgaga <210> 413 <211> 499 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 413 gagcagttc actcacaaat ctaaatcttc gatcaagcct ccatatttgt ttctacttca tcggtgattt cccatcatgg acgcagttaa tgccgacgtg aagaggcgat cgcagctgct aaggcacata caaggagaat gcgacggcat ggattcaact cattcaattc cgcactggt tacatcacct attctcagcg tctcctgaag gtcacacgaa gtggtggcga cctagcaaag gtggaggtgg gtgatcactg gcttggacgg acttgatatc gacgaagacc aagatcatcg aggacggaac cccagacccg caaagacaaa caagcctcaa tttcgaaggc gagggcaaga agatgttctg tctcctgcaa tgggaagttc gtacgttatt gacaaacatc cgtcattgac tcgtcatcag acttctctag gagtccttga tctctggtga aagacagtga tatgtgaaga atgcttgttg ggtgcgacaa 120 180 240 300 360 420 480 499 <210> <211> <212> <213> 414 497
DNA
Eucalyptus grandis <400> 414 gccggtgaca ggaggctgct gacgggactg ggaactacac agcaagcggt acgtgataag aagaagaaga ccaacctgac gaccygagog tgaagggcgg gagcgattct tggccaaaaa gccgttgccc tccgtgttgg taccaggaca ccctctatgt ggcacagtcg acttcat gcagtcgacg gacqatctcg gataaagacc tttcatcggc cttcaccacc cataaccttc tgccgatcta ccacaaaaac gcggtgattc gaggcagtgg ggggtgtaca gacgggcgga ttcgagtccg cagaacaccg tctgccattt cgccaattct cggatgtggt cggcggcgcc gagagaacgt ccaccaccat ccactgtcgc Ccgcccttc acgaatgcga ttgtcaagtg ggtggcggcg ggagaagagc gcaggtgccg catcaccggc ggtgcttggc aaaccaccag catcctcgcc cttaattgcc 120 180 240 300 360 420 480 497 <210> 415 <211> 295 <212> DNA <213> Eucalyptus grandis <400> 415 gcgggacgta caagacgatt gcggaggcga ggatcatcat ctacgtgcga gccgggaggt agaagacgaa cctcatgttc atcggcgatg aaagtgtagc cgacaagatg accacgttcc gtttcattgc ccgcgacatg accttcgaga taaagaaggc acgaggagga ggaagggcag acaccgcctc actacgccgg gccggagagc agtggccgga taacttgaag gtggggaaga aacggtcata acgggcggca cttcgcggcg agcggagccg gccggagaag cacca <210> 416 <211> 433 <212> DNA <213> Eucalyptus grandis <400> 416 ttcggggtga atgtgatcgc ccatggccgc ccgaggtacg gacgatctgg caagcatgga ggccgtgatg gtgttcttit gatgaccacg gctgcgttgc gaagaactgg cgattcgagt atagaccacg ccatggtcag ggcgtagttc tcg ccatgtagga tcgggatgct tcgaaatgcc gcttggccat caaagatgaa ggacgtagag ctcctacccg caacatgtac tcctttagat ggtgttctgg gggcttccgg gtcaacggtc cgtgtcctga gagagccacc acaaccctcg gcctcgagat ttggggtcct gcatagatgt ccgtagatgt tagccaacga gcctggtgct agtacatctt ctggagcggc tgcggttctg tgctcttttg cgcattcacg tgctgcacct tctccggccc 120 180 240 300 360 420 433 <210> 417 <211> 414 WO 00/22092PCIZ90I6 PCT/NZ99/00169 <212> DNA <213> Eucalyptus grandis <400> 417 ggcgtccgag gtgatgcggc gcgcccgttg gcgaggatca acagttctgg aagacggcag gcactcacgg tagaactggc gctgcaccta tagaatgccg ttggggcccc gctgtgtttt tgtgaatgta gcagagccgc agccctggat cggtataggc ctgcgttccc ggagtacgag cgtgatcgga ggaacccgat gcaaggaaga tgtgaaccca tgagttccca aaagataaaa cgcgtacaat ggccacggnt gtctngggct cccccagcca gtgttctggc gactttcgca tctatggtgc gtgtcctggt agggcgacag ataaatccat cactatcatc ccgggtcact acaccagctc catagatgtc agcccgcgat cttgttgacc cgccggtgat accg <210> <211> <212> <213> 418 382
DNA
Eucalyptus grandis <400> 418 ggctgatgga cgtggcggga agtatccaga gtggctaggg tacaggcgga catagtggtg aggcgataaa gaaggcgccg ggaggtacga ggaggataac gcgatgggaa gggcagaacg cgttccacac cgcctccttc ggaggagggg gagagagaga gcgaaggacg gagagcagtg ttgaaggtgg gtcataacgg tc gagtaccgaa gggagctgct gagcgagcgg gccggaggat ggaagaagaa gcggcaaaag gcccgacggc ggacatgccg gacgtacaag catcatctac gacgaacctc tgtagccgac ggcggcgctg gcggcggagg acgat tgcgg gtgcgagccg atgttcatcg aagatgacca 120 180 240 300 360 382 <210> 419 <211> 247 <212> DNA <213> Eucalyptus grandis <400> 419 ccgccgtgct ccaggactgc gacatccacg togtcaccgc ccagggccgn gatgatccca gcaggatcgg cgcgacatca natctcttgg gaaggccatg gaagatgtac tcgaggacgg tcaaccc cgagacgccc caaccctggc cagcgcaaca accagaacac aggtatagtg atccaaaagt cagtgaaagg gagcttccaa acttatctgg tgataatgca gaccgccata agcgacgtga <210> <211> <212> <213> 420 471
DNA
Eucalyptus grandis <400> 420 gcgacatgac ctttgagaac taggagctga ccatggcgtg aCgtCeaCLC gaatcgccag tctttggcaa cgcagccgtg ccaagcaaaa gaacaccatc tttcgatcca tgcttgccag acatgggcga tcacattcac tacgccgggc gtctataggt ttcttccgtg gtcatccaaa acggcccaga atcgtcgccg ecatygaaga cccgaagggt cggagaagca gcagcatcgt aatgcgacat agagcaacat accgcaagga ctccagatct tgcactcgag ggctggagtg ccaggcggtg tggctatcag ctacgggacc ctatgcccgg ccccaaccag cgaggcatct ggtcgtgtac gaacggagac gctctccggg gacacgctct gttgacttca aagcccatgg aacaccggca aaaggaagca atgttgtcct t <210> 421 <211> 371 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 421 ngaagacgaa cctcatgttc aaagtgtagc cgacaagatg gtttcattgc ccgcgacatg tggctctccg ggtaggagct aggacacgct ctacgtccac ccgttgactt catctttggc ggaagcccat g atcggcgatg accacgttcc accttcgaga gaccatggag tcgaatcgcc aacgcagccg ggaagggcag acaccgcctc actacgccgg tggtctatag agttcttccg tggtcatcca aacggtcata cttcgcggcg gccggagaag gtgcagcatc tgaatgcgac aaagagcaac acgggcggca agcggagccg caccaggcgg gttggctatc atctacggga atctatgccc 120 180 240 300 360 371 <210> 422 <211> 349 <212> DNA <213> Eucalyptus grandis <400> 422 ccgggaccta caacgagacg gcgacgggaa gacgaagacc ctttcagcac ggcgaccttc tcgtgaacac agccggcgcg tctcggtgat ataccgctgc accgccagtt ctaccgcgac gtgctgctgg atcgtcaccg gctgttgcgg gcgaagcacc gcctttgatg tgcgacatca acaagagcaa gcagcaagaa gtaaagggtt aggcagtggc cgtaccagga cgggcacgat gtggaacgtg cttcatcgac tattgcgaga gttccggtct cacgctctat cgacttcat ctgatgtacg ggcactccga gacatgatgt gggtccgacc gcgcactcga 120 180 240 300 349 <210> 423 <211> 357 <212> DNA <213> Eucalyptus grandis <400> 423 ggaggttgct gcaaatgccg ggaacgggac gtgcaagacg gccggtttat catatacgtg ggaagaagac gaacgtgatg gcaagagcat cttcgacaac ccgggttcat cgctcgggac gtgacggcga atctcggagg cgagccggaa ttcgtagggg atgacgacgt atccgttcga tacaggcgga ccatcaagaa ggtacgagga acgggaagag tccacaccgc gaactgggct cgtgacggta ggcgccggac agataatctg caacaccatc gtccttcgct gggcccgcca tcgaaggacg tacggtaccc aaggtgggga at ctccggcg gccaccggag agcacca 120 180 240 300 357 <210> <211> <212> <213> 424 346
DNA
Eucalyptus grandis <400> 424 gggggcggcg gcggcgagta atgccggcgg cggaggtaca tacaagacga tcgcggaggc atctacgtgc aagccgggag aacctcatgt tcatcggcga gccgacaaga tgaccacgtt tccagagtgg ggcggacata gataaagaag gtacgaagag tgggaagggc ccacaccgcc ctaggggaga gtggtggcga gcgccggaga gataacttga aaaacggtca tccttcgcgg gagagaggga aggacggagc gcagtggccg aggtggggaa taacgggcgg cgagcg gctgctggac gaacgggacg gaggatcatc gaaqaaqacq caaaagtgta 120 180 240 300 346 <210> 425 <211> 577 <212> DNA <213> Eucalyptus grandis <400> 425 gcgacggccg gaccaccacc atcatcaccg cttcgagtcc gccaccgtcg cggtggttgg gcgaccggac gtgaagggcg gcttcaccac cgagcgattc ttggccaaaa gcataacctt WO 00/22092 WO 0022092PCT/NZ99/00I 69 ccagaacacc atcagccttt ccgccaattc agctgtcgtc catggttact atgcaggatt cggtcgtcca aatcgacccg gntggccctt tacgaatgcg tttgtcaagt atccaagact gctcaaggac gctgagacca tggaaggagt gtgggttggc caaaccacca acatcctcgc gcttaattgc gtgacatcca gaattgaccc acgatctccg actcgaggac acgagtggag ggccgttgcg ctaccaagat cggcacagtc tgcccgcaag gaaccaaaac atcagtgaag agtgattatg tgggacc ctccgggttg accctctatg gacttcatct ccaaaccctg acgggaatcg agcagtttcc caatcatcga gcgccgatct tccacaacaa ttggtaacgc gccaaaagaa tgatccaaaa caacgtacct tctcggacgt 180 240 300 360 420 480 540 577 <210> 426 <211> 283 <212> DNA <213> Eucalyptus grandis <400> 426 aaagaccggg gtgtacagag catcggcgac gggcggacca caccaccttc gagtccgcca aaccttccag aacaccgccg cgatctatct gccatttacg agaacgtgca ccaccatcat ctgtcgcggt gcccttcaaa aatgcgacat ggtgccgaag aagaagacca acctgacttt caccggcgac cggagcgtga agggcggctt gcttggcgag cgattcttgg ccaaaaacat ccaccaggcc gttgccctcc gtgttggtgc cctcgcctac cag 120 180 240 283 <210> <211> <212> <213> 427 345
DNA
Eucalyptus grandis <400> 427 ccaccgtcgg agtgcttggc cgggtccaga cgcccaccag agaactgcga gttcctggga acaagtcctg ccacatcgag tccaggactg ccagatcctg atgctgtcac agcccatggc gacggattca gcggtggcat aaccaggaca ggcaatgtgg gtccgccccc cggaccgacc tggcaaccgg tccggtcgga cgctctatgc acttcatctt ggcaggtcaa ccgcgcagtc gctcacgatc cagaacaccg cagcgatttc tcggtcatcg ccacgccctc cggcagtact tgggaactcg gcctcctact gcccgagaag ggcgagagca gacag <210> 428 <211> 478 <212> DNA <213> Eucalyptus grandis <400> 428 tgagcagttc actcacaaat ctaaatcttc gatcaagcct ccatatttgt ttctacttca tcggtgattt cccatcatgg acgcagttaa tgccgacgtg aagaggcgat cgcagctgct aaggcacata caaggagaat gcgacggcat ggattcaact tacatcacct attctcagcg tctcctgaag gtcacacgaa gtggtggcga cctagcaaag gtggaggtgg gtgatcactg gcttggacgg acttgatatc gacgaagacc aagatcatcg aggacggaac cccagacccg caaagacaaa gcagcctcaa tttcgaaggc gagggcaaga agatgttctg tctcctgcaa tgggaagttc gtacgttatt gacaaacatc cgtcattgac tcgtcatcag acttctctag gagtccttga tctctggtga aagacagtga tatgtgaaga atgcttgttg ggtgcgac <210> 429 <211> 335 <212> DNA <213> Eucalyptus grandis <400> 429 tttttttttt tttttttttt taatcnccaa cttacagaaa ctcccccaga aagaaaggac acattccaaa ttattgaatt cacaacacaa caatactcat ttttcacagc ccttcagtgt WO 00/22092 WO 0022092PCT/NZ99/0OI 69 aagacactcc agtggacttc aaccaagtcc tcttcgcctc tgctgggctg gtgatgacat ttcccgcgcc tggtcccttg ttcatgtact cgccgctcca caccgaccac cctgtgggat ctccctgaat cagctcggcc acggngaact gataacctgg ccacttcacc cgcttgctcg ccccgtagta canagtcttg agggcatgat caatg <210> 430 <211> 361 <212> DNA <213> Eucalyptus grandis <400> 430 cttacagaaa ctcccccaga aagacactcc agtggacttc tcttcgcctc tgctgggctg ttcccgcgcc tggtcccttg cgccgctcca caccgaccac c taatctccaa aagaaaggac aaccaagtcc gtgatgacat ttcatgtact cctgtgggat acattccaaa caatactcat ctccctgaat gataacctgg ccccgtagta caatgtgatc ttattgaatt ttttcacagc cagctcggcc ccacttcacc cagagtcttg accaatgttt cacaacacaa ccttcagtgt acggngaact cgcttgctcg agggcatgat gattgcatca <210> 431 <211> 368 <212> DNA <213> Eucalyptus grandis <400> 431 aacttacaga aactccccca gtaagacact ccagtggact cttcttcgcc tctgctgggc cgttcccgcg cctggtccct atcgccgctc cacaccgacc cccacagt tcaaatctcc gaaagaaagg tcaaccaagt tggtgatgac tgttcatgta accctgtggg aaacattcca accaatactc ccctccctga atgataacct ctccccgtag atcaatgtga aattattgaa atttttcaca atcagctcgg ggccacttca tacagagtct tcaccaatgt ttcacaacac gcccttcagt ccacggtgaa cccgcttgct tgagggcatg ttgattgcat 120 180 240 300 360 368 <210> 432 <211> 324 <212> DNA <213> Eucalyptus grandis <400> 432 cccaaccaga acaccggcat gaggcatcta aaggaagcat gttgtgtaca tgttgtccta aacggagact ttgcgctaga gcagccgtcg gcctacgtgt gcaaacaaat tcacagtcgc ttcgatccat cccgacgtac catgggcgat cactttgtat gaaatggcct gcag gcttgccaga Ctcgggcggc cacattcacc tacggagagt ggtttccgag tcgtcgccgc catggaagat ccgaagggtg acatgaacga tcatcacatc tccagatctc gtactcgagg gctggagtgg tgggcctggg cacaacagag 120 180 240 300 324 <210> 433 <211> 460 <212> DNA <213> Eucalyptus grandis <400> 433 gcccgacggc ggcggcggcg agtatccaga ggacatgccg gcggcggagg tacaggcgga gacgtacaag acgattgcgg aggcgataaa catcatctac gtgcgagccg ggaggtacga gacgaacctc atgttcatcg gcgatgggaa gtogctaggg catagtggtg gaaggcgccg ggaggataac gggcagaacg gagagagaga gcgaaggacg gagagcagtg ttgaaggtgg gtcataacgg gggagctgct gagcgagcgg gccggaggat ggaagaagaa gcggcaaaag WO 00/22092 CNZ90I6 PCT/NZ99/00169 tgtagccgac aagatgacca cgttccacac tccgaatttc taatgttcaa actctcgact tttgtctaaa taatttattt tttacgaaac cgcctccttc ggtaaatttc tgtgctcata aagctaggcc aaaaattata aataatcttt aaatcgaacc 360 420 460 <210> 434 <211> 344 <212> DNA <213> Eucalyptus grandis <400> 434 aggacggaac tgggaagttc cccagacccg gtacgttatt caaagacaaa gacaaacatc gcagcctcaa cgtcattgac gcgatgggtt catagcccag aggccgtcgc actccgtgtc aagacagtga tatgtgaaga atgcttgttg ggtgcgacaa gacatatggt agtgcagaca aagaggcgat aaggcacata gcgacggcat cattcaattc tccagaacac agtcagtcat cgcagctgct caaggagaat ggattcaact cgcaactgtt tgccgggccg caac cctagcaaag gtggaggtgg gtgatcactg gctgtgaatg cagaaacacc 120 180 240 300 344 <210> 435 <211> 295 <212> DNA <213> Pinus radiata <400> 435 acgagctcga gacatgacct tcgagaacac ctgcgtgtgg gatcagatct ctcggctttc accctttacg cccattccct tcgtcagttt gatttcatct tcggcaactc cgccgtcgtt cccctggaga atcagacgat tctttacacc ggaaggaccc tatcgctgca tacagagaat tttcaggatt gctcagggca gcgaacacca ggcggtggcc gcttcaaggg ttaccaggac gcaacatcta tggcaccgta gcaatttgct ggcgcggaga ggcaggaccc caatg 120 180 240 295 <210> 436 <211> 332 <212> DNA <213> Pinus radiata <400> 436 tgcaggtatt tgttgcacgt aagctgtggc tctacgggtg gttaccaaga cacactttat tcggaacagt ggacttcatt atgctcgaca aggcatgccc cttatcaaaa taccggcatc gacatgagct ggggccgatt gttcactctc tttggcaatg aatcaagtga tcaatacata ttatgaacag ttgcagcgat tgaggcagtt cagccgtggt atgtaatcac at tgcagggcct ttatcgatgc ttacagagaa tttacaggag tgcccaagga gacaagcatc agtattattg tgtgacgtgt tgtaacattt aggaatcatc <210> 437 <211> 301 <212> DNA <213> Pinus radiata <400> 437 gcggccccag agaaaagtgg taagagatat ggagaacgtg gaggtgggta aaacgaagac ggccacaatc gttacaggga 9cayaaatLL cacattcgct gctgtaggga agggatttat aggcccggac aaacatcagg cggtggctct c gtgatcaagg taatatcatg gatagacoga ggcacaagac tcgagtaggt tgaagaaggg ttgattggag tccaccacur atggcgttcg caoatcaatc aacgttacaa aaggcatgga tcaattcagc tcaacacagc agtgttatat 120 180 240 300 301 <210> 438 <211> 242 WO 00/22092 WO 0022092PCT/NZ99/00 169 <212> DNA <213> Pinus radiata <400> 438 gaagaaggga acgtacaagg agaacgtgga ggtgggtaaa acgaagacta atatcatgtt gattggagaa ggcatggagg ccacaatcgt tacagggagc agaaatgtga tagacggatc caccactttc aattcagcca cattcgctgc tgtagggaag ggatttatgg cacaagacat ggcgttcgtc aacacagcag gcccggacaa acatcaggcg gtggctcttc gagtaggatc ag 120 180 240 242 <210> <211> <212> <213> 439 255
DNA
Pinus radiata <400> 439 acttaactcc ggtggaaggt aaccatgaat ctccttcaat gaattttgag actgtaaatg aactggcctc gcttgagcta ttaatcactc gataccccgc ccatttgacc cgatttgtag tgcccgcacc agggccgcgg ttcatgtatt ccccatagta tagggtgctg agagcgaagg atccattcca ttcgagccaa ccggcaggtt gaatcaggtc gcccagaaag gactgcatga agacagtgcg agagt <210> 440 <211> 362 <212> DNA <213> Pinus radiata <400> 440 gtggactgca gccatgaatc tgagaacttg tgattacccg gggcctgtat tcatgtattc tccagccagc ccgcaggctg gaatactcct tccacggcct tcggaatcgg gagtgatctt gt tccttgtatg atacccggcc tccataatac aataacatcg ccccagatat gcagttgtga aattctccaa catttcacgc aaagtttgca tctagatagg gttgggaatg atggaagttc cagtaaactt ggttagcagt atgcaaaact actgcatgta agcttttcac ctgtgttctg gctcgcctct tgctgctcct tccattccat aa ccgt tcgg cggaacaagg gttcggatca <210> 441 <211> 286 <212> DNA <213> Pinus radiata <400> 441 aagaaaacta atatcatgtt cgttggagat cgaaatgtga aggacaattt cacaaccttt ggattcatcg ctcgcgacat gaccttcgag gtggccctgc gtgtgggatc agatctttcg caggacaccc tttacgccca ttcccttcqt ggtatggatg cgttctgcaa aacacggcag gctttctatc caagttttta tcacagtggt gaccggaaac ctgttgctgt gactggaaat gacccgcgaa gcaccaggcg gatgcagctt caagggttac cagaga <210> 442 <211> 302 <212> DNA <213> Pinus radiata <400> 442 ggagaagagc cagacgagat acgtaattca tataaaagca ggagtttatg cagagaatgt ggagttgcac aagaagaaaa ctaatatcat gttcgttgga gatggtatgg atgtcacagt ggtgaccgga aaccgaaatg tgaaggacaa tttcacaacc tttcgttctg caactgttgc 120 180 WO 00/22092 WO 0022092PCT/NZ99/00I 69 tgtgactgga aatggattca tcgctcgcga catgaccttc gagaacacgg caggacccgc gaagcaccag gcggtggccc tgcgtgtggg atcagatctt tcggctttct atcgatgcag ct 240 300 302 <210> 443 <211> 466 <212> DNA <213> Pinus radiata <400> 443 gaaatcgtga cgaactatcg gcaactgtcg gcggggccgg gagaggtzgcg tatcggaatt ttccacaact aaccccataa gaattccacc tcaccggatc gggtgaacgc agatgcatca cctttctcgg gcaggatcga gctccattct ctgcccacgg gagcaagacg attgtctgcc ggacggtttt ggctgttgcc acaccaggac aggcaacgtc cgtccgccct gcgattggat aatctcatgt cagattcccg gt tgcgcgag ctcagagtcg accctatacg gacttcatat cgccaggtgc ccgggtcaga ttgttggaga gcgtcggcac acattgcgtt atagcgatct cgcacgccct t tggaaacgc cgtctaatct ctactg tggcatggat acacggctct cgagaatact ctcagcattc ccgccagttt agcggccatc t tccgaagcq 120 180 240 300 360 420 466 <210> 444 <211> 345 <212> DNA <213> Pinus radiata <400> 444 ggatacacta caaaaacgat aaaaattgaa agcgaactac aatttatcaa tccgtcgatg tgaattctcc cactgtaaat gccatttcac acggtttcca acaaagtttt caaagcaaaa agaaaaagaa agacacagac tagtcgatgc ttgctcgcct gtagcggagc gctccatccc atacagacac gaacttcttc cagtggactg cttgagaact ctggccctgt attccagcca tacagacaca gacgaacttc aaaagtttgg cgaagtcgct caaccaggaa tttccttgta tttgatcacc cgatacccag attcatatat tctccataat gcccg 120 180 240 300 345 <210> <211> <212> <213> 445 183
DNA
Pinus radiata <400> 445 acgcgcattc nccgcgccaa ttctacaggg agtgtaacat tttgggcact gtagatttca tatttgggaa tgccgcagtg gtgtttcaga gctgcaatct gatgcccagg aaacccggtg caaatcagaa aaatgccatc acagcacagg gcagaactga tccgaaccaa aacacaggaa ctt 120 180 183 <210> <211> <212> <213> 446 264
DNA
Pinus radiata <400> 446 acgctgcaat cttgcagcga tataacaccg attgatctga tcctactcga agagccaccg cctgatgttt gtccgggcct gctgtgttga cgaacgccat gtcttgtgcc ataaatccct tccctacagc agcgaatgtg gctgaattga aagtggtgga tccgtctatc acatttctgc tccctgtaac gaitgtggcc tccatgcctt ctccaatcaa catgatatta gtcttctttt tacccacctc cacgttctcc ttgt 120 180 240 264 <210> 447 <211> 417 WO 00/22092 WO 0022092PCTINZ99/00I 69 <212> DNA <213> Pinus radiata <400> 447 agagaatgca acatctatgg atgggcgtat agggtgtcct tgatcccaca cgcagggcca catgtcgcga gcgatgaatc gaaattgtcc ttcacatttc gaacatgata ttagttttct tatatgaatt acgtatctcg caccgtagat ggtaaccctt ccgcctggtg catttccagt ggtttccggt tcttgtgcaa tctggctctt gttgcattct gaagctgcaa cttcgcgggt cacagcaaca caccactgtg ctccacattc ctccggagcc ctgtaaaact cgatagaaag cctgccgtgt gttgcagaac acatccatac tctgcataaa ttctccactg gacgaaggga ccgagagatc tctcgaaggt gaaaggttgt catctccaac Ctcctgcttt cctctgt 120 180 240 300 360 417 <210> 448 <211> 404 <212> DNA <213> Pinus radiata <400> 448 attatatctc agctgacata tgcagagcag agaaaagaaa gcattttaga gtacagcaga actacgtgag cagccctcca agatgaactg ggccaccgtg caggccacgt cacccgcctc aaaataaggt atccagtgcg aatatatatt gcccttaatt ataatataca ataaaaacga aatggataaa gcaagccctg aagtctccga aaaaattaca tattggccac cgtcagcctt tcccagtgga cttcctgcgc cgccggggcc accactccaa gcatacaatg ctggcttgca aatttcagaa tggcaaccat cgctttgaaa actgttcata ccat gaactttgac atattctatt aaataaataa ttagcaccag atcctgtaac tattccccat <210> <211> <212> <213> 449 173
DNA
Pinus radiata <400> 449 gcctcgctgg agctcttaat cgctcgatat cctggccatt tgacccggcc tgcagtgttt gcgccagggc cgcggttcat gtattcccca taatagaggg tactcagtgc gaaggttcca ttccattcga gccaacccgc tggctgaatc atgtcgccaa gaaacgattg cat <210> 450 <211> 398 <212> DNA <213> Pinus radiata <400> 450 ggctctgaga gtaggagcgg ggacacactg tacgtacatt agtggacttc atctttggca.
aaaacccctg cccaactcca gaacaccggc atctccatcc caagcctc tatcgcgctt cctgcaaact tttttggatg attttgeagc ctctccgcca acgcagccgt agatcacggt acgactgcag acctcgggag atattattga cttttaccgt attttacaga ggtgttgcag gacggctcag agtgacggcg gccctggaaa CCCtgccg tgcagcgtca gaatgcgaca aagtgcacca ggcaggaagg gcggcggatc ttatactctc tcggttacca tctacggcac tgttcgccag accccaacca.
ttgctcccgt gc acggt at a <210> 451 <211> 404 <212> DNA <213> Pinus radiata <400> 451 agagaatgca acatctatgg caccgtagat gttgcattct ctgtaaaact gacgaaggga WO 00/22092 WO 0022092PCT/NZ99/001 69 atgggcgtat tgatcccaca catgtcgcga gaaattgtcc gaacatgata tatatgaatt agggtgtcct cgcagggcca gcgatgaatc ttcacatttc ttagttttct acgtatctcg ggtaaccctt CCgCctggtg catttccagt ggtttccggt tcttgtgcaa tctggctctt gaagctgcaa cttcgcgggt cacagcaaca caccactgtg ctccacattc ctccggagcc cgatagaaag ccgagagatc cctgccgtgt tctcgaaggt gttgcagaac gaaaggttgt acatccatac catciccaac tctgcataaa ctcctgcttt ttct <210> <211> <212> <213> 452 394
DNA
Pinus radiata <400> 452 agagaatgca acatctatgg atgggcgtat agggtgtcct tgatcccaca cgcagggcca catgtcgcga gcgatgaatc gaaattgtcc ttcacatttc gaacatgata ttagttttct tatatgaatt acgtatctcg caccgtagat ggtaaccctt ccgcctggtg catttccagt ggtttccggt tcttgtgcaa tctggctctt gttgcattct gaagctgcaa cttcgcgggt cacagcaaca caccactgtg ctccacattc ctcc ctgtaaaact cgatagaaag cctgccgtgt gttgcagaac acatccatac tctgcataaa gacgaaggga ccgagagatc tctcgaaggt gaaaggttgt catctccaac ctcctgcttt <210> 453 <211> 428 <212> DNA <213> Pinus radiata <400> 453 attatatctc agctgacata tgcagagcag agaaaagaaa gcattttaga gtacagcaga actacgtgag cagccctcca agatgaactg ggccaccgtg caggccacgt cacccgcctc aaaataaggt atccagtgcg atcatcct aatatatatt gcccttaatt ataatataca ataaaaacga aatggataaa gcaagccctg aagtctccga aaaaattaca tattggccac cgtcagcctt tcccagtgga cttcctgcgc cgccggggcc accctccaac gcatacaatg ctggcttgca aatttcagaa tggcaaccat cgctttgaaa actgttcata catccggcag gaactttgac atattctatt aaataaataa ttagcaccag atcctgtaac tattccccat ggtcaataat <210> <211> <212> <213> 454 329
DNA
Pinus radiata <400> 454 gcaaatcaga aaaatgcaat acttccattc acaactgcaa ttcccaacat atctggggag tatctagacg atqttattca caaactttgt attatggaga qtgaaatggg ccgggtatcg caccgcacag gatcactccc gccgtggaag Scc t gcgqq c atacatgaat ggtaatcac ggcagaactg gatgccgacc gagtattccc tqqctqqaat acaggcccag atccgaacca ttgttccggt gaacggttta ggaatqqaag gagcagcaac gaacacagga gaaaagctca catgcagtcc ttttgcattg tgctaaccgc <210> <211> <212> <213> 455 358
DNA
Pinus radiata <400> 455 ctacgatcct ctccaaacaa cgtagtgcca aacgtgatcg tggctaagga tggctctgga aatttcaaaa cgatttcaca agccatagct gcggccccag agaaaagtgg taagagatat WO 00/22092 WO 0022092PCT/NZ99/00 169 gtgatcaagg tgaagaaggg aacgtacaag gagaacgtgg aggtogggtaa aacgaagact aatatcatgt tgattggaga aggcatggag gccacaatcg ttacagggag cagaaatgtg atagacggat ccaccacttt caattcagcc acattcgctg ctgtagggaa gggatttatg gcacaagaca tggcgttcgt caacacagca ggcccggaca aacatcaggc ggtggctc <210> 456 <211> 195 <212> DNA <213> Pinus radiata <400> 456 cgtggaggtg ggtaaaacga agactaatat catgttgatt ggagaaggca tggaggccac aatcgttaca gggagcagaa atgtgataga cggatccacc actttcaatt cagccacatt cgctgctgta gggaagggat ttatggcaca agacatggcg ttcgtcaaca cagcaggccc ggacaaacat caggc <210> 457 <211> 405 <212> DNA <213> Pinus radiata 180 240 300 358 <400> 457 ttcgcggccg cgtcgacgta gcccgatcca tctcccgaga atggctatct gcaggagatc cgtgatcgtg gctaaggatg ggccccagag aaaagtggta gaacgtggag gtgggtaaaa cacaatcgtt acagggagca attctctggc tacgcctccc ggaggctcct gctctggaaa agagatatgt cgaagactaa gaaatgtgat aatagcaaag gtccgattcc acgatcctct tttcaaaacq gatcaaggtg tatcatgttg agacggatcc ctggcgcagg atcaaggatg ccaaacaacg atttcacaag aagaagggaa attggagaag accac agtcagtaat attttccttc tagtgccaaa ccatagctgc cgtacaagga gcatggaggc <210> 458 <211> 326 <212> DNA <213> Pinus radiata <400> 458 cgtggaacat ggatggccta gcgaagcgac gtcactagtc catgaatctc cttgtatgaa attacccgat acccggccca atgtattctc cataatacaa gcaggctgaa taacatcgtc caagacactc tgttaatccg ttctccaaca tttcacgcgg agtttgcaat tagata gacagcaaac gaaacatagt caaaacatta tccacataat cgacaccggt ggactgcagc gtaaacttgc tcgcctcttg agaacttgtg ttagcagttg ctgctcctgg gcctgtattc gcaaaacttc cattccattc cagccagccc <210> 459 <211> 360 <212> DNA <213> Pinus radiaca <400> 459 tcgacctacg atcctctcea r t a gatatgtgat caaggtgaag agactaatat catgttgatt tgtgatagac ggatcnccac atggcacaag acatggcgtt aacaacgtag ";acaagcca aagggaacgt ggagaaggca tttcaattca cgtcaacaca tgccaaacgt tagccgcggc acaaggagaa tggaggccac gccacattcg g c a 9CCcgg gatcgtggct cccagagaaa cgtggaggtg aatcgttaca ctgctgtagg acaaacatca aaggatggct agtggtaaga ggtaaaacga gggagcagaa gaagggattt ggcggtggct <210> 460 WO 00/22092 PCT/NZ99/00I 69 <211> 438 <212> DNA <213> Pinus radiata <400> 460 cgtggaacat ggatggccta gcgaagcgac gtcactagtc catgaatctc cttgtatgaa attacccgat acccggccca atgtattctc cataatacaa gcaggctgaa taacatcgtc cacggcctcc ccagatatgt gtgatcttgc agttgtga caagacactc tgttaatccg ttctccaaca tttcacgcgg agtttgcaat tagataggac tgggaatgag gacagcaaac tccacataat gtaaacttgc ttagcagttg gcaaaacttc tgcatgtaaa cttttcaccg gaaacatagt cgacaccggt tcgcctcttg ctgctcctgg cattccattc ccgttcggga gaacaaggtc caaaacatta ggactgcagc agaacttgtg gcctgtattc cagccagccc atactccttc ggcatcggga 120 180 240 300 360 420 438 <210> <211> <212> <213> 461 380
DNA
Pinus radiata <400> 461 agagaatgca acatctatgg atgggcgtat agggtgtcct tgatcccaca cgcagggcca catgtcgcga gcgatgaatc gaaattgtcc ttcacatttc gaacatgata ttaagttttc tatatgaata cgtatctcgt caccgtagat ggtaaccctt CCgcctggtg catttccagt ggtttccggt ttcttgtgca gttgcattct gaagctgcaa cttcgcgggt cacagcaaca caccactgtg actccacatt ctgtaaaact cgatagaaag cctgccgtgt gttgcagaac acatccatac ctctgcataa gacgaaggga ccgagagat c tctcgaaggt gaaaggttgt catctccaac actcctgctt 120 180 240 300 360 380 <210> <211> <212> <213> 462 439
DNA
Pinus radiata <400> 462 agagaatgca acatctatgg atgggcgtat agggtgtcct tgatcccaca cgcagggcca catgtcgcga gcgatgaatc gaaattgtcc ttcacatttc gaacatgata ttagttttct tatatgaatt acgtatctcg gttcgtgtaa tttccactg caccgtagat ggtaaccctt ccgcctggtg catttccagt ggtttccggt tcttgtgcaa tctggctctt gttgcattct gaagctgcaa Cttcgcgggt cacagcaaca caccactgtg ctccacattc ctccggagcc ctgtaaaact cgatagaaag cctgccgtgt gttgcagaac acatccatac tctgcataaa ttctccactg gacgaaggga ccgagagatc tctcgaaggt gaaaggt tgt catctccaac Ctcctgcttt cctctgtaat 120 180 240 300 360 420 439 <210> 463 <211> 441 <212> DNA <213> Pinus radit <400> 463 gtgaacatac cttccaccaa attatacaat ggtccqacac tataacagcg ctactgttgc canaacacag ccccggttcc atcacgggag acacgtcgtc tatgaccacg ctggccgcca atcttcggga acggcctctc tacggcgcgg tgacagcgca ggccttcata ggctoggact agtcaattcg tctgccggg Cttcttcggg ttatttcaaa cctctacgag g acgctggaag cccocsaccca.
ccttacttca gcggtgggca tgcagcttct gactgttata gggtgcagcc gagacggcgc acggtaaagc tcgccagaaa gacaagcagt tgggcgcgca tcgaaggctc tgcatgggat agactccacc gttgggtaca cattacgttt ggccttgaga ggacactttg cgtcgatttc ttccgatacc 12 0 180 240 300 360 420 441 WO 00/22092 WO 0022092PCT/NZ99/00 169 <210> 464 <1>481 <212> DNA <213> Pinus radiata <400> 464 tgatccctga cattactgtg aagctgtgaa cagtgcgccg gggtttacgg ggaaacggtt ccggtatgga taaaacggtg tcacaattta cgatgtcgcc taacattccg aaacacattt acagcgattt ttctgccttc cgcacacgct ccgccaattc t tcaaagctgg gactactcgg cgaatccccc atcaccggtt acggttggag caggggcca c tacagctgcg tacagaaatt atcagaaatc aaaagaaatt gcagtaagac ctgcatatgt tgaatgggga agactcatca ctttcgagag gcagaataga ctctctatca tgtgatcaag gaatctcgtg gccgtctctg cggcttcata agccgtggcc ccaccaggac gggcacccac agcattcagc atacaggccg tttgtgggcg cccggcccgc gcccgtgaca ctgagagtag acgctctaca gacttcatct <210> 465 <211> 505 <212> DNA <213> Pinus radiata <400> 465 gcaattctct ggccatgttc cccttcaggg tcatgattat caacaaaaag gcgacgcctt atcaagaatt ttatgaccat ggtggttatc ttcccgagat atgctgttgt ggccttggat atgcaccttc cctgctaagc acgaaaatgt cacgatttca gaaaaactat tgtagcagca cagatcttgt catgtacaga ttggcagagg tatggattga cgcaggcttt ggaagtggca agcagaaggt aggaagaaga ggcaa tctgcaacac caaacaactt caggggaaga ttcatggagg tgaaactgcc agtataagag atatcatcta ccaatctcat cacaaaatct tacagtccca aatgaacaat ggcgcagcat cgttgctgcc catagttgat tgtgaaaaca gattgttggt gacattggtg tcgtctcctc gctcttcgga gaatttcctc atgcaacctg gctgtcaacg ggcgtgtata gatggcat tg 120 180 240 300 360 420 480 505 <210> 466 <211> 361 <212> DNA <213> Pinus radiata <400> 466 cggaggctcc tacgctcctt ggctctggaa aattcaaaac aagagatacg tgatcaaggt aagaagacaa atattatgct agaaatgttg tagacggttc gggtttatgg cacaagacat g tccaaacgac aattacagaa gaagaagggg gatcgganaa caccactttc ggcgttcgtc atcgtggctg gccatagctg acgtacaagg ggcatggaag aattcctcta aacaccgcag acgtgatcgt cggccccgga agaacgtgga ccacgatcgt cactagctgc gtccagataa ggctcaggat gaaaagctct agtgggcaaa tacagggagc tgtagggaag gcatcaagcg 120 180 240 300 360 361 <210> 467 <211> 402 <212> DNA <213> Pinus raudiata <400> 467 CCCCCgagct acgcctcccg ttcgattcca tcaagga-tga t-tttccttca tggctatctg caggagatcg gaggctccta cgctcctttc caaacgacat cgtggctgac gtgatcgtgg ctcaggatgg ctctggaaaa ttcaaaacaa ttacagaagc catagctgcg gccccggaga aaagctctaa gagatacgtg atcaaggtga agaaggggac gtacaaggag aacgtggaag WO 00/22092 WO 0022092PCT/NZ99/00I 69 tgggcaaaaa gaagacaaat attatgctga tcggagaagg catggaagcc acgatcgtta cagggagcag aaatgttgta gacggttcca ccactttcaa ttcctctaca ctagctgctg tagggaaggg gtttatggca caagacatgg cgttcgtcaa ca <210> 468 <211> 397 <212> DNA <213> Pinus radiata 300 360 402 <400> 468 tgatccctga cattactgtg aagctgtgaa cagtgcgccg gggtttacgg ggaaacggtt ccggtatgga taaaacggtg tcacaattta cgatgtcgcc taacattccg aaacacattt acagcgattt ttctgccttc tcaaagctgg gactactcgg cgaatccccc atcaccggtt acggt tggag caggggccac tacagctgcg atcagaaatc aaaagaaatt gcagtaagac ctgcatatgt tgaatgggga agactcatca ctttcga ctctctatca tgtgatcaag gaatctcgtg gccgtctctg cggct tcat a agccgtggcc agcattcagc atacaggccg tttgtgggcg cccggcccgc gcccgtgaca ctgagagtag <210> 469 <211> 349 <212> DNA <213> Pinus radiata <400> 469 gcaggacccg cgaagcacca tatcgntgca gcttcaaggg tacagagaat gcaacatcta tttcaggatt gcaatttgct gctcacggca ggcaggaccc accgcagccc cagacctggc ggcggtggcc ttaccaggac tggcaccgta ggcgcggaga caatgagaac tccagtgaag ctgcgtgtgg accctatacg natttcatct cccctggaga actggaattt agctcgttcg gatcagatct cccattccct tcggcaactc atcagaagat ccattcagaa atgcatatc ctnggctttc tcgtcagttt cgccgtcgtt tctttacacc ctgtaatgtg 120 180 240 300 349 <210> 470 <211> 375 <212> DNA <213> Pinus radiata <400> 470 cggaggctcc tacgctcctt ggctctggaa aattcaaaac aagagatacg tgatcaaggt aagaagacaa atattatgct agaaatgttg tagacggttc gggtttatgg cacaagacat gtggctcttc gtgta tccaaacgac aat tacagaa gaagaagggg gatcggagaa caccactttc ggcgttcgtc atcgtggctg gccatagctg acgtacaagg ggcatggaag aattcctcta aacaccgcag acgtgatcgt cggccccgga agaacgtgga ccacgatcgt cactagctgc gtccagataa ggctcaggat gaaaagctct agtgggcaaa tacagggagc tgtagggaag gcatcaagcg 120 180 240 300 360 375 <210> 471 <211> 367 <212> DNA <213> Pinus radiata <400> 471 tgatccctga cattactgtg aagctgtgaa cagtgcgccg gggtttacgg ggaaacggtt ccggtatgga taaaacggtg tcacaattta cgatgtcgcc taacattccg aaacacattt tcaaagctgg gactactcgg cgaatccccc atcaccggtt acggttggag caggggccac atcagaaatc aaaagaaatt gcagtaagac ctgcatatgt tgaa tgggga agactcatca ctctctatca tgtgatcaag gaatctcgtg gccgtctctg cggcttcata agccgtggcc agcattcagc atacaggccq tttgtgggcg cccggcccgc gcccgtgaca ctgagagtag 120 180 240 300 360 WO 00/22092 WO 0022092PCT/NZ99/001 69 acagcga <210> 472 <211> 446 <212> DNA <213> Pinus radiata <400> 472 cggaggctcc ggc tc tgga a aagagatacg aagaagacaa anaaatgttg gggtttatgg gtggctcttc caagacacat tacgctcctt aattcaaaac tgatcaaggt atattatgct tagacggttc cacaagacat gtgtaggatc tgtacgcgca tccaaacgac aattacagaa gaagaagggg gatcggagaa caccactttc ggcgttcgtn agaccaatca ttctct atcgtggctg gccatagctg acgtacaagg qgcatggaag aattcctcta aacaccgcag qtgttatatc acgtgatcgt cggccccgga agaacgtgga ccacgatcgt cactagctgc gtccagataa gctgcaagat ggctcaggat gaaaagctct agtgggcaaa tacagggagc tgtagggaag gcatcaagcg tgcagcgtac 120 180 240 300 360 420 446 <210> 473 <211> 345 <212> DNA <213> Pinus radiata <400> 473 ggcgcagact ccaccattat aaagcgttgg gtacatataa agaaacatta cgtttcagaa gcagtggcct tgagaatcac gcgcaggaca ctttgtatga ggctccgtcg atttcatctt acaatggtcc cagcgctact cacagccccq gggagacacg ccacgctggc cgggaacggc gacacggctg gttgcagtca gttcctctgc tcgtccttct cgccattatt Ctctccctct ggactcccgg acccaacggt attcgcctta cttcatcgcc cgggggcggt gggcagacaa tcgggtgcag cttcttgggc tcaaagactg ttatatcgaa acgag <210> 474 <211> 268 <212> DNA <213> Pinus radiata <400> 474 aactaatatc atgttcgttg gagatggtat ggatgtcaca gtggtgaccg gaaaczcgaaa tgtgaaggac aatttcacaa cctttcgttc. tgcgactgtt gctgtgactg gaaacggatt catcgctcgc gacatgacct tcgagaacac ggcaggaccc gcgaagcacc aggcggtggc cctgcgtgtq ggatcagatc tctcggcttt ctatcgatgc agcttcaagg gttaccagga caccctttac gcccattccc ttcgtcag <210> 475 <211> 316 <212> DNA <213> Pinus radiata 120 180 240 268 <400> 475 gccagacgag atacgtaatt catataaaag acaagaagaa aactaatatc atgttcgttg gaaaccgaaa tgtgaagac aatttcacaa gaaacggatt catcgctcgc gacatgacct aggcggtggc cctgcgtgtg ggatcagatc gttaccagga caccct caggagttta gagatggtat cctttcgztc tcgagaacac tctcggcttt tgcagagaat ggatgtcaca tgcgactgtt ggcaggaccc ctatcgatgc gtggagttgc gtggtgaccg gctgtgactg gcgaagcacc agcttcaagg 120 180 240 300 316 <210> 476 <211> 440 WO 00/22092 WO 0022092PCT/NZ99/OO 169 <212> DNA <213> Pinus radiata <400> 476 cgagaatagc cagacgagat ggagttgcac ccgacgaaaa ggtgaccgga aaccgaaatg tgtgactgga aacggattca gaagcaccag gcggtggccc cttcaagggt taccaggaca caacatctat ggcaccatag caatctcctg gcgcggaggc acataattca caaatatcat tgaaggacaa t cgctcgcga tgcgtgtggg ccctttacgc atttcatctt tataaaagca gttcattgga atttacaacc catgaccttc atcagatctc ccattccttt cggcaactcc ggagtttatg gatggcatgg tatcgttctg gagaacacgg t cggc c tt ct cgtcagttct gccgtcgttt, cagagaatgt atgttacagt caactgttgc caggacccac ataagtgcaa acagaaaatg ttcaggattg 120 180 240 300 360 420 440 <210> 477 <211> 357 <212> DNA <213> Pinus radiata <400> 477 gaaacctgag acgtacttaa tgagactgta aatgactggc acccgatttg tagtgcccgc ctgagagcga aggatccatt aaggactgca tgaagacagt aacgagctct tcactggagc ctccggtgga ctcgcttgag accagggccg ccattcgagc gcgagagtac caggtctggg aggtaaccat ctattaatca cggttcatgt caaccggcag tctttccatg gctgcggtca gaatctcctt ctcgataccc attccccata gttgaatcag gcctcccaag cattacagtt caatgaattt cgcccatttg gtacagggtg gtcgcccaga atatgcctcg ctgaatq 120 180 240 300 357 <210> 478 <211> 318 <212> DNA <213> Pinus radiata <400> 478 cagaactgca ctgtgaccgc cgcctcggac taccttggca ggccgtggag aaattactcg gacttgattc agccagcggg ttggttggaa tactacgggg aatacatgaa cagcggcccc gcggggtatc aggtgattaa gaaatccaag attgaaggca attcatgg ctggttccag tgaaaacatc gttcgaggcg cgcactgtgt tcatgaaatc ttatctctac tggaatggca gcttcgctct gagcactctg ggcgcgggca ctgccaatcg ggtcagatgg gaggccaaga aatttacagt gtctcaattc <210> 479 <211> 271 <212> DNA <213> Eucalyptus grandis <400> 479 gaaattcttt ggtaacttga tqqa~tqq tc tcata atctattctc gcttacaaaa ngacattgtc cgtcagcatt tgagaatgtt gattcaggag tggatgctgg ttcgtgagaa ataaggagaa gggngacata cagcaaagga attatgctcg gtttgtggag aggaaagttt agatgggata tgggccgtgc ttgcrtggtt cttaagtgga gaaaagcttg tatctgtcga tggtcgtcat tattatacca gatatgatta a <210> 480 <211> 301 <212> DNA <213> Eucalyptus grandis <400> 480 WO 00/22092PC/Z90I6 PCT/NZ99/00169 gatgctggat tatgctcggc ttgtggayag gaaagtcttg gaactggctc tgatcatatt cgtcaganag atgggatatg ggctgtgctt. gcctggttat ctattctcgc ttacaaaaat aaggagaact taagtggaga aaagcttgta rtgtcgagg acattgtccg tcagcattgg gtgacatatg gtcgtcatta ttataccaga tatgattatg agaatgttga ttcaggagca gcaaaggaac tgatgggata cttggtccaa ctgcaatcat Ctctctctga agtcaaccag ':210> 481 <211> 287 <212> DNA <213> Eucalyptus grandis <400> 481 aaattctttg gtaacttgaa tggatgctgg tggaactggc tctgatcata ttcgtgagac atctattctc gcttacaaaa ataaggagaa ggacattgtc cgtcagcatt gggtgacata tgagaatgtt gattcaggag cagcaaagga at tat gc tcg agatgggata cttaagtgga t ggt cgt cat actgatggga gtttgtggag aggaaagttt tgggctgtgc ttgcttggtt gaaaagcttg tatctgtcga tattatacca gatatgatta tacttgg 120 180 240 287 <210> <211> <212> <213> 482 285
DNA
Eucalyptus grandis <400> 482 gaccatatac gtgaaaaaga tggtatctgg tacaagaaca aggaaaatat caatggtgga cagcactggg caacttatgg tcgccactat gcaggggcag caaaggaact aatggcatac gttaatgaga ttgtcaaggg agtatgttcg gctgttttgg aagcttgtat tacactcgtt ttggtccggt gatgtgtcaa catggctttc catccttgcg cagttgaaga tattgttcgc atgattatga gaatgttgac tgcaatcttc cctcggtgaa atgtt <210> 483 <211> 427 <212> DNA <213> Eucalyptus grandis <400> 483 cgttcgtgcg ctgatatttt aaaatggtga cgttccaggt ggcacctccg gcctccgcaa tttgtgcaat caacattcta gtttctggtg atgggcgtta gctgcaaatg gagtaaggcg gtgtcagctg tgatccgtga tctgcac ctctctccgg gtcgcgagtg gaaggtgaaa tgccctttca tttctctaag tgtctgggta aagagt tggg tttccctggc gagaccgcgc gtttttgtcc gctgagaaag gatgctatcc ggtcagaatg catgatggtc cggcggaagc ccttcgatgg agccccatta tccaaggagc agatcataat gattactttc caaggcacag gaatcggcag ccagaagccc cttgcaaaat tacactcgtt aaagatgtca cactcctgcc gagcatttat 120 180 240 300 360 420 427 <210> 484 <211> 408 <212> DNA <213> Eucalyptus grandis <400> 484 aaaaaactgt catctcgcct tgctcacttc atcctccctg tttatgaaaa tggtgacgtt cgatggccag aagcccggca cctccggcct ccattacttg caaaattttg tgcaatcaac aggagctaca ctcgttgttc tggtgatggg ctcaccacct ccaggtgtcg ccgcaagaag attctatgcc cgttatttct ttcctggcac cgagtggaga gtgaaagttt ctttcagctg ctaaggatgc catcatcgac ccgcgccctt ttgtccagcc agaaagtcca tatccagatc WO 00/22092PCNZ/016 PCT/NZ99/00169 ataataaaga tgtcagctgc aaatggagta aggcgtgtct gggtaggtca gaatggatta ctttccactc ctgccgtgtc agctgtgatc cgtgaaagag ttgggcat <210> 485 <211> 243 <212> DNA <213> Eucalyptus grandis <400> 485 gttgaagcca taccctactt ttctggcggt tcagctgccc ttgatgtggt tgctcaacat gggtggaaat tctttggtaa cttgatggat agttttggaa ctggctctga tcatattcgt tg9 ctcaagggag ttgccaagaa catgcctaca ctaaatctga agttttttga ggtgcctaca gctggattat gctcggtttg tggagaggaa gagaaagatg ggatatgggc tgtgcttgct <210> 486 <211> 372 <212> DNA <213> Eucalyptus grandis <400> 486 ccggaggtaa gcgaantcgg cgcgaccctt acgatggcca ttttgtccag ccccattact tgagaaagtc caaggagcta tgctatccag atcataataa tcagaatgga ttactttcca tgatggatcc aa acagaaatgg gaagcccggc tgcaaaattt cactcgttgt agatgtcagc ctcctgccgt tgcacgttcc a cc tcc ggcc tgtgcaatca ttctggtgat tgcaaatgga gtcagctgtg aggtgtacgc tccgcaagaa acattttatg gggcgttatt gtaaggcgtg atccgtgaaa gagtggagac ggtggaaagt ccctttcagc tctctaagga tctgggtagg gagttgggca <210> 487 <211> 511 <212> DNA <213> Eucalyptus grandis <400> 487 9caccatcat tgtcgcgagt agaagttgaa tatgcccttt tatttctcta cgtgtctggg gaaagagttg aatcccggtg gctcctgagg cgacatcctc ggagaccgcg agtttttgtc cagctgagaa aggatgctat taggtcagaa ggcatgatgg gtccccatga cgatcactga cctgtttctg cccttcgatg cagccccatt agtccaagga ccagatcata tggattactt atccaaggct ggattttgga taagatgtat cgaatcggca gccagaagcc acttgccaaa gctacactcg ataaagatgt t cc act cc tg acaggagcat atcaagtata gaaaatggtg cggcacctcc atttggtgca ttgtttctgg cagct--gcaaa ccgtgtcagc ttattctgac acatggaaaa acgttccagg ggcctccgca ntcaacattt tgatgggcgt tggagtaagg tgtgatccgt ggcaagtcat tggtggacct 120 180 240 300 360 420 480 511 <210> 488 <211> 465 <212> DNA <213> Eucalyptus grandis ->488 gcaccatcat tngacatcct gtgtcgcgag tggagaccgc aagaagttga aagtttttgt tatgcccttt cagctgagaa tatttctcta aggatgctat cgtgtctggg taggtcagaa ccctgtttct 9cccttcgat ccagccccat agtccaagga ccagatcata tggattactt gcgaatcggc ggccagaagc tacttgcaaa gctacactcg ataaagatgt tccactcctg agaaaatggt ccggcacctc attttgtgca ttgtttctgg cagctgcaaa ccgtgtcagc gacgttccag cggcctccgc atcaacattt tgatgggcgt tggagtaagg tgtgatccgt 120 180 240 300 360 WO 00/22092PC/Z9016 PCT/NZ99/00169 gaaagagttg ggcatgatgg atccaaggct acaggagcat ttattctgac ggcaagtcat aatcccggtg gtccccatga ggattttgga atcaagtata acatg 420 465 <210> <211> <212> <213> 489 514
DNA
Eucalyptus grandis <400> 489 ctggcaccat aggtgtcgcg gcaagaaggt tttatgccct gttatttctc ggcgtgtctg gtgaaagagt ataatcccgg ctgctcctga catcgtcatc agtggagacc gaaagttttt ttcagctgag taaggatgct ggtaggtcag tgggcatgat tggtccccat ggcgatcact Ctccctgttt gcgcccttcg gtccagcccc aaagtccaag atccagatca aatggattac ggatccaagg gaggattttg gataagatgt ctgcgaatcg atggccagaa attacttgca gagctacact taataaagat tttccactcc ctacaggagc gaatcaagta atga gcagaaaatg gcccggcacc aaattttgtg cgt tgtt t Ct gtcagctgca tgccgtgtca at ttat tctg taacatggaa gtgacgttcc tccggcctcc caatcaacat ggtgatgggc aatggagtaa gctgtgatcc acggcaagtc aatggtggac <210> 490 <211> 442 <212> DNA <213> Eucalyptus grandis <400ctgatatttt acgttccagg ggcctccgca tcaacatttt gatgggcgtt ggagtaaggc gtgatccgtg gcaagtcata 490 tCtctctccg tgtcgcgagt agaaggtgaa atgccctttc atttctctaa gtgtctgggt aaagagttgg atcccggtgg gtttccctgg ggagaccgcg agtttttgtc agctgagaaa ggatgctatc aggtcagaat gcatgatgga tc ccggcggaag cccttcgatg cagccccatt gtccaaggag cagatcataa ggattacttt tccaaggcta cgaat cggca gccagaagcc acttgcaaaa ctacactcgt taaagatgtc ccactcctgc caggagcatt gaaaatggtg cggcacctcc ttttgtgcaa tgtttctggt agctgcaaat cgtgtcagct tattctgacg <210> 491 <211> 520 <212> DNA <213> Eucalyptus grandis <400> 491 ctgatatttt acgttccagg ggcctccgca tcaacatttt gatgggcgtt ggagtaaggc gtgatccgtg gcaagtcata ggtggacctg tctctctccg gtttccctgg tgtcgcgagt agaaggtgaa atgccctttc atttctctaa gtgtctgggt aaagagttgg atcccggtgg ctcctgaggc ggagaccgcg agtttttgtc agctgagaaa ggatgctatc aggtcagaat gcatgatgga tccccatgag gatcactgat ccggcggaag cccttcgatg cagccccatt gtccaaggag cagatcataa ggattacttt tccaaggcta gattttggaa aagatgtatg cgaatcggca gccagaagcc acttgcaaaa ctacactcgt taaagatgtc ccactcctgc caggagcatt tcaagtataa gaaaatggtg cggcacctcc ttttgtgcaa tgtttctggt agctgcaaat cgtgtcagct tattctgacg catggaaaat <211> 418 <212> DNA <213> Eucalyptus grandis <400> 492 ctgatatttt tctctctccg gtttccctg ccggcggaag cgaatcggca gaaaatggtg WO 00/22092 WO 0022092PCT/NZ99/00I 69 acgttccagg ggcctccgca Ccaacatttt gatgggcgtt ggagt aaggc gtgatccgtg tgtcgcgagt agaaggtgaa atgccctttc atttctctaa gtgtctgggt aaagagttgg ggagaccgcq agtttttgtc agctgagaaa ggatgctatc aggtcagaat gcatgatgga ccc t tcga tg cagccccat t gtccaaggag cagatcataa ggattacttt tccaaggcta gccagaagcc acttgcaaaa ctacactcgt taaagatgc ccactcctgc caggagcatt cggcacctcc ttttgtgcaa tgtttctggt agctgcaaat cgtgtcagct tattctga 120 180 240 300 360 418 <210> 493 <211> 424 <212> DNA <213> Eucalyptus grandis <400> 493 gagaacttat tgttaaagat atgaaacaaa catgtgaaag ttcgctgagt ctgctcttcc agcagatcgg ccagtaaatc agctaaagct tcttgagaat gtaaagacga atggttgccc cccatcttca aacnaataac t ttg aatagagaga gtctagcagg tgtcatttag cctccatctt ctctcccagt cttctgagcc gaataccctg tcaagatgcg gttgccaact ttagcaagcc ggagagcttg tttcaaggga ggttcccgag gtgcttcgat cctctcatga gtttagagaa catcatgtga agtgcgacat tccttctcat agacggaaaa atggaaccat agtcacccat atctctcata tgaccgtggg tcaccagagg attgctcaat tnaggcgcga caacaggatc <210> 494 <211> 257 <212> DNA <213> Eucalyptus grandis <400> 494 gttctttgtt actccatcag attctgttgc accctacttc tccggaggct taaagggagt ggatgttgtt gctaaacatt taaatttgaa ctttggtaac ttaatggatg ctggattatg tgggtcagac catatac tattattgct gcaaatgctg ttgaagcaat tgccaggagc atgccaacat cagctgcctt gttttttgag gttccaacgg gctggaaatt ttcagtttgt ggggaagaaa gtttcggaac 120 180 240 257 <210> 495 <211> 483 <212> DNA <213> Eucalyptus grandis <400> 495 ggtaaaaatc cggtaaattc cctgagaatc tggttgcacc acaaatatcg attcatcagc cttcagagag aatcaacatt gac ttactgtggc ctgcattttt tctcccagtt ttccgagcct a at accc tgg atgaacaaca agaagartgc ctcataatca gaaagcggga gaaagcttga tttgctggat gttcccgaga tgcttagaaa tttgatgcat agttggacca tatctggtat tctatgtgat gagcaacttc ccttctcata ggcgaaaaac tggaaccatc caggacaaac agtaccccat aataatgacg cacggttggg cacaagagga ctgctctatg aagtcgtgac aacaggatcc ccccttgaca cagttccttt accatatgtc gcagatcgcc gccaatgctt taaagacgaa ccatcttcga ttatattcaa atctggttga gctgctcctg acccaatgct <210> 49G <211> 353 <212> DNA <213> Eucalyptus grandis <400> 496 tgacaatctg gttgacttca gagagagaag attgcagttg gaccaagtat cccatcagtt WO 00/22092 WO 0022092PCT/NZ99/001 69 cctttgctgc atgtcgccca tctccttatt tctcacgaat agcatccatc tcctgaatca acattctcat atgctgacgg acaatgtcct tttgtaagcg agaatagata atgatcagag ccagttccaa aagttaccaa agaatttcca aatcatatct caacagatac accaagcaag aactttcctc ccctgtaggc ggtataataa aagcttttct cacagcccat tccacaaacc acctcaaaaa tgacgaccat ccacttaagt atcccatctt gagataatcc act 120 180 24 0 300 353 <210> 497 <211> 442 <212> DNA <213> Eucalyptus grandis <400> 497 gccaaccgca tatttgtaga cctaaggagg attttggagg gtggcacgga tgggtttggg gctgctgatg gtgatgctga tcagattctg ttgctattat ggcttaaagg gagttgccag atttaaattt gaagtttttt atgctggatt atgttcagtt agagcttggt gggtcaccca caagtcaagt tcgtaatatg tgctgcaaat gagcatgcaa gaggttccaa tg gcacaagaga gatccaaatt CCtcancatg gttctcggaa gctgttgaag catcagctgc cgggctggaa gctcattatt tgacatatgc agccccccaa aaaggttctt caatacccta cttggatgtt attctttggt gaactgcaca aaaggagctg atttggtgct tgttactcca cttctccgga gttgctaaac aacttaatgg <210> 498 <211> 364 <212> DNA <213> Eucalyptus grandis <400> 498 gccaaccgca cctaaggagg gtggcacgga gctgctgatg tcagattctg ggcttaaagg catt tatttgtaga attttggagg tgggtttggg gtgatgctga ttgctattat gagttgccag agagcttggt gggtcaccca caagtcaagt tcgtaatatg tgctgcaaat gagcatgcca gcacaagaga gatccaaatt cctcagcatq gttctcggaa gctgttgaag acatcagctg gctcattatt tgacatatgc agccaccaga aaaggttctt caatacccta ccttggatgt gaactgcaca aaaggagctg atttggtgct tgttactcca ct tct Ccgga tgttgctaaa 120 180 240 300 360 364 <210> 499 <211> 365 <212> DNA <213> Eucalyptus grandis <400> 499 ccgtgtcagc tgtgatccgt ttattctgac ggcaagtcat acatggaaaa tggtggacct aaacaataaa agaatatcta gtgtcacaag Ctttacgggg catct gaaagagttg aatcccggtg gctcctgagg attgcagaaa ccagagggtc aagtcaaL L.
ggcatgatgg gtccccatga cgatcactga atctccctca aattcgatgt ttL gactLtcL.
atccaaggct ggattttgga taagatgtat tgtggatatt tgaggttttt qqCqatCCqd acaggagcat atcaagtata gaaaatacaa gctgcaattg gattcagcca aayc~gcttt 120 180 240 300 3 365 <210> 500 <211> 390 <212> DNA <213> Eucalyptus grandis <400> 500 ggctacagga gcatttattc tgacggcaag tcataatccc ggtggtcccc atgaggattt tggaatcaag tataacatgg aaaatggtgg acctgctcct gaggcgatca ctgataagat 120 WO 00/22092 WO 0022092PCT/NZ99/00I 69 gtatgaaaat acaaaaacaa taaaagaata tctaattgca gaaaatctcc ctgatgtgga tattgctgca attggtgtca caagctttac ggggccagag ggtcaattcg atgttgaggt ttttgattca gccagtgact atgttaaatt aatgaagtca atttttgact tccaggcgat ccgaaagctg ctttcatctc caaattttac cttctgctat gatgctctcc atggagttgc tggagcatat gccaaccgca tatttgtaga <210> <211> <212> <213> 501 341
DNA
Eucalyptus grandis <400> 501 ggtaggtcag aatggattac tgggcatgat ggatccaacg tggtccccat gaggattttg ggcgatcact gataagatgt aaatctccct gatgtggata tcaattcgat gttgaggttt tttccactcc ctacaggagc gaatcaagta atgaaaatac ttgctgcaat ttgattcagc tgccgtgtca atttattctg t aa cat gga a aaaaacaata tggtgtcaca cagtgactat gctgtgatcc acggcaagtc aatggtggac aaagaatatc agctttacgg 9 gtgaaagagt ataatcccgg ctgctcctga taattgcaga ggccagaggg <210> 502 <211> 600 <212> DNA <213> Eucalyptus grandis <400> 502 gctaaaagaa actgtcatct ccctgtttct gccagaagcc acttgcaaaa ctacactcgt taaagatgtc ccactcctgc ttctctttgg ttatctggat aaaacaaata cgccttgctc gaaaatggtg cggcacctcc ttttgtgcaa tgtttctggt agctgcaaat cgtgtcagct tggcatccaa atgaagtgct aataaaaaaa agttccccac acgttccagg ggcctccgca tcaacatttt gatgggcgtt ggagtaaggc gtgatccgtg ttgtataacc ctctaacaaa gaaataattt cacctttcct tgtcgcgagt agaaggtgaa atgccctttc atttctctaa gtgtctgggt aaagagttgg ctttgactat atacgtacac tgcagacaaa ggcaccatca ggagaccgcg agtttttgtc agctgagaaa ggatgctatc aggtcagaat gcatgatgta gcaatcacaa aatctatcat atttgaaaaa tcgacatcct cccttcgatg cagccccatt gtccaaggag cagatcataa ggattacttt agctatcttt tgcagcaata cagggatcca <210> 503 <211> 360 <212> DINA <213> Eucalyptus grandis <400> 503 gcaaaactgt ttacatgagg tgatcacggt tggggcagat cttccacaag aggagccaat catactgctc tatgtaaaga aaacaagtC9 tgaLct catcaacagg atccttatat ggtcggtaaa cgcccggtaa gcttcctgag cgaatggttg t~aC~iaa tcaaattcat aatcttactg attcctgcat aatctctccc caccttccga atcgaatacc cagcatgaac tggcgaaagc ttttgaaagc agtttttgct gcctgttccc crggtgctta aacatttgat gggatctatg t tgagagcaa ggatccttct gagaggcgaa gaaatggaac gcatcaggac <210> 504 <211> 395 <212> DNA <213> Pinus radiata <400> 504 gttcttggca agaggttttt tgtgactcca tcagattctg ttgccatcat tgcagcaaat gcagttgaag ccattccata cttcagctct ggattgaaag gtgttgcaag aagcatgccg WO 00/22092PC/Z9016 PCT/NZ99/00169 acatcagctg cacttgatgt agttgcaaaa agtcttaatc ttaggttttt cgaagtgccc actggctgga agttttttgg aaatttaatg gatgctggaa tgtgttctgt ttgtggtgaa gagagtttcg gcactggttc cgaccatatc cgagagaagg atggaatctg ggcagtttta gcatggcttt caattctagc ttacaaaaat aaggataacc ttgatggcgg gaagcttgtc actgtagagg acatagtccg taaccattgg gcttc <210> 505 ':211> 477 ':212> DNA ':213> Pinus radiata <400> 505 180 240 300 360 395 tccatctcca ccctacgagg caagagcact gt caacggct caatcgatcc gcaatccttt atcctgctga aacatcaaca ctacaaccac gccagaagcc ataccgagaa caaccctcgt tcaagatcgc cgaccccggc ccgccagtca acggtgggcc tatcatgacc tggtacgtcc cttcgtccaa tgtcggaggt ggccgcaaac cgcctcgaac caaccctggt ggccccagag cgcttcaaca gggctgcgaa qctatcctcg gacggtcgat ggcgtgaaga atcattcgcc ggaccggaca agcgttaccg tccaagaggt agagggtgaa atgcgatgcc accactccga agctatacat agtacaaggc atgactttgg acaagatctt ttctaccaag ggtgttccag tgg ac ctgg t gcctaccgtg tggcaaagac cgatggtggt tatcaagtac cgagcgc 120 180 240 300 360 420 477 <210> <211> <212> <213> 506 436
DNA
Pinus radiata <400> 506 tggaaacaga accatcaacc aaacatctgg cctcacttcc taattaaatg tgacatgagc gtgtataata gtggcgacca caagcttccc gccatcaagg aaactgccca gattccatcc caccacaaac agaacacatt gcacctcgaa aaacct ggatctttgt ttaattaatt tccttggcag taagaagccc ttatccttat ttctctcgga ccagcatcca attcaaattc tgttaacttc cacctgcatc aatggttacg ttttgtaagc tatggtcgga ttaaatttcc atcggcatcc ggggagagaa aacattttca gactatgtcc tagaattgaa accagtgccg aaaaaacttc acgacaccag gactgcagct tagtcatagc tctacagtga agccatgcta aaactctctt cagccagtgg 120 180 240 300 360 420 436 <210> 507 <211> 473 <212> DNA <213> Eucalyptus grandis <400> 507 tgataatgaa gggaagattc caacccggta tacctgtttg gctcacttgc tcttaccccg cgaggtgtct gtgttggaga ggaaagccat ttgagcaacc gaagcactcc gaattccccg gctaaaaatc agagacctct aggcactaaa gcttaggact ttagagtgag acgagggctc gcatcaagtt tggatgggca tctccacaaa gaagcaacaa atgagcaaat tccatatact gttgataatg gaccatctct cacttatggc atttgacaga attctatggt tggcactggt cacagccagc agttccccct aaagagggtg tggatcccgt ccggagagtt ctagatgagc gaagtcaccc ctcttccaga aaagcagctg cttctttctc tgaagtattt gtggaagaaa acttcgggca tcatctatgt agcagatgct ccatagttgg caccattaca aat 120 180 240 300 360 420 473 ':210> 508 ':211> 379 <212> DNA <213> Eucalyptus grandis ':400> 508 WO 00/22092 WO 0022092PCT/NZ99/00I 69 gctcatcaag cgttagggac atggaaaatc cattgaagcc tgcagtgatt tagagtgagg cgagggctcg cctcccaaga ttgctggact cagagggaca cgaaagccag gaagtgctgc ttgataatga accatctct tcctggtcat tcagcatata tggccgagcg attttgaagc caacacagct aagagggtgt tgagggactt tttggacatc aggacacagt ctatatagac aatccctggt gaagtatttc cacccaatgt agcaatgagg ctcgagagca ccacagaagc gataatgaag aacccggtat tcgaccagcg tcaaatttgc tcaaagctag agtatgcaga ggaagattct acctgtttga <210> <211> <212> <213> 509 459
DNA
Eucalyptus grandis <400> 509 aaagaaaana accncagagg tctctaccta accgttaaga gaaagctcgc gtgcgataga tcctcgacgc tccagtcgca ccgagcaacg tcgctttcac gacaaattct ccaaaagtga aagctgggag atcaggtgaa gtaatggatg gccgttttgt agcaggccct ctcccgtaag gagagagaga gatcggcggc caggaagatc tatcattgta agctgtggag tcccaatatt taatcgtacc gacacaattc gagagagaga ctcgagctcc cagacggtgg tctccatcaa ttggcaggat acaatcggt ctccgacacc agagcgagaa tgtcgaccgc ggcggtcttc tgaaggcatc ttctatctgc gtgattggat cgactttctc agaagagaga ctcggtttgc cctccggcgg ttcacgagtt taatttttcg ccacgttgat <210> 510 <211> 268 <212> DNA <213> Eucalyptus grandis <400> 510 tcaccaggaa gatccagacg gtggtgaagg catcttcacg agttgacaaa ttctccaaaa gtgatatcat tgtatctcca tcaattctat ctgctaattt ttcgaagctg ggagatcagt aatccaggtg aaagctgtgg agttggcagg atgtgattgg atccangttg atgtaatgga tggccgtttn gttcccaata ttacaatcgg tccccttgtg gttggtgccc tgcgccctgt aacagatctt cctctggatg ttcatctg <210> 511 <211> 293 <212> DNA <213> Eucalyptus grandis <400> 511 gnaaaaaaga gagatgtcga ccgcctcgct gcggcctcga gctccggcga tcctcccttc agatccagac ggtggtgaag gcatcttcac ttgtatctcc atcaattcta tctgctaatt tggagttggc aggatgtgat tggatccacg ttgctcctcg gccggccgag gagttgacaa tttcgaagct ttgatgtaat acgctccagt caacgtcgct attctccaaa gggagatcag ggatggccgt cgcagattcg ttcaccagga agtgatatca gtgaaagctg ttt <210> 512 <211> 423 <212> DNA <213> Eucalyptus grandi1s <400> 512 gtggagttgg caggatgtga ttggatccac aatattacaa tcggtcccct tgtggttggt gatgttcatc tgatgattgt ggaacctgaa gctgacatag tcagtgtgca ttgtgaacaa gttgatgtaa tggatggccg ttttgttccc gccctgcgcc ctgtaacaga tcttcctctg cagcgagtac cggatttcat caaggctgga acttctacca tccacttgca tcgcacggtc WO 00/22092 WO 0022092PCTNZ99/00 169 aaccaaatca aaagtctggg agctaaagct ggagttgtcc tgaaccctgc taccocacta actgctatag aatatgttct tgatgtggtt gatctggtgt tgatcatgtc ggngaaccct ggctttggtg ggcaaagCtL tatcgagagc caagtgacga aaatatcaga cttgagaagg atg <210> 513 <211> 508 <212> DNA <213> Pinus radiata <400> 513 cacagcagcc ccagtgagca taaaattgcc tatgacggaa aaatcttacc ggattgtcat agcttcaggg aagaatcaga aaactgttta cacgggccca attgagggat ccgtcaatgt aatggtgcag attggagcac cttatggtca ttcacttttc aatgctggca tccctttggt ggacggt cgg cactgaaaat tgtcatcaga attggttgca ctgtgattca caaaccctct atgtggaggc tgcggcctgg ggaaagtg tgggtttggt ggaagggaac ctttgcgaat tatggacatc gagtttgagg tgattatgtg tgccaaagac agtggcagtg tagacctgtc acagagaagg ctggcttcag atggatgggc aagcataccc gagccatttg aattggcaag aaacctggaa tgaacaaggg ggg ttat ccc aggcgaaata atttcgttcc aggcattctt caaaagctgg atctcatcaa cttctataga 120 180 240 300 360 420 480 508 <210> 514 <211> 502 <212> DNA <213> Pinus radiata <400> 514 cacagcagcc ccagtgagca taaaattgcc tatgacggaa aaatcttacc ggattgtcat agcttcaggg aagaatcaga actgttatcc cacgggccca attgagggat ccgtcaatgt aatggtgcag attggagcac cttatggtca ttcacttttc aatgctggca tttggtggaa ggacggtcgg cactgaaaat tgtcatcaga attggttgca ctgtgattca caaaccctct atgtggaggc tgcggcctgg gt tgggtttggt ggaagggaac ctttgcgaat tatggacatc gagtttgagg tgattatgtq tgccaaagac agtggagtga tagacctgtc acagagaagg ctggcttcag atggatgggc aagcataccc gagccatttg aattggcaag aacctggaac tgaacaaggg gggttatccc aggcgaaata at tt cgt tcc aggcattctt caaaagctgg atct catcaa ttctatagaa 120 180 240 300 360 420 480 502 <210> <211> <212> <213> 515 447
DNA
Pinus radiata <400> 515 tcccgtcaca ttttttaaat catgtcaaca gcagcgatat ccattctatg ggtgtctgcc aggcccccaa ztggcgaact agtagacaag ttctccaaaa tgcaacatta ggtgaccagg tgatgtcatg gatgggcgtt ttcattttgt caatatgcgc gtagtccgtt crtcaaggaa ctgacatcat tcaaagctgt ttgtgccaaa tgccact tgccttgagg cacggtcgct ctggggaaag agttctctct tgtctctcct ggagttggca tattaccatt tctagagatc atggtgggtt aagcataaca acagtgaagg tctattcttt ggttgcgatt ggacctctgg gattcttagg ctcagagcac tggcctttgc catcttcccg ctgcaaattt gggttcatgt tggtggctgc <210> 516 <211> 403 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/O00169 <400> 516 tgcatttcga gtttaccaag aggtctaag atcoattctt gctatggtgg gttctcagag aagaagcata acatggcctt tctacagtga aggcatcttc ccttctattc tttctgcaaa gcaggttgcg attgggttca atatcccgtc aggcatt-a cacccattct tgcaggcccc ccgagtagac ttttgcaaca tgttgatgtc acatttttta acagcagcga atgggtgtct caattggcga aagttctcca ttaggtgacc atggatgggc aatttcattt tatcaatat-, gccgtagacc actcttcaag aaactgacat aggtcaaagc cgt tgttgccttg cgccacggtc gttctgggga gaaagttctc cattgtctct tgtggagttg 180 240 300 360 403 <210> 517 <211> 379 <212> DNA <213> Pinus radiata <400> 517 attctatggg tgtctgccgt gcccccaatt ggcgaactct tagacaagtt ctccaaaact caacattagg tgaccaggtc atgtcatgga tgggcgtttt tacgacccgt aacagatttg gtgtaccaga ttttatcaa agtccgttct tcaaggaaag gacatcattg aaagctgtgg gtgccaaata ccactggatg agggaaagaa ttctctctac tctctccttc agttggcagg ttaccattgg tacatttgat gcataacatg agtgaaggca tattctttct ttgcgattgg acctctggtg gattgttgaa gcctttgcag tcttcccgag gcaaattttg gttcatgttg gtggctgcat ccggaacaac 120 180 240 300 360 379 <210> <211> <212> <213> 518 404
DNA
Pinus radiata <400> 518 attctatggg tgtctgccgt gcccccaatt ggcgaactct tagacaagtt ctccaaaact caacattagg tgaccaggtc atgtcatgga tgggcgtttt tacgacccgt aacagatttg gtgtaccaga ttttatcaag agtccgttct tcaaggaaag gacatcattg aaagctgtgg gtgccaaata ccactggatg gctggtgcag ggggaaagaa ttctctctac tctctccttc agttggcagg ttaccattgg tacatttgat acattgttag gcataacatg agtgaaggca tattctttct ttgcgattgg acctctggtg gattgttgaa tgt c gcctttgcag tcttcccgag gcaaattttg gttcatgttg gtggctgcat ccggaacaac <210> 519 <211> 705 <212> DNA <213> Pinus radiata <400> 519 gaagcaacca tactctcaag tttggttaga cctgtctgaa aaatggaagg caacacagag tgcatatgga catcatggat ttcagagttt gaggaagcat ctcttgatta tgtggagcca aqqctqccaa acgacaattaQ ctggagtggc agtgaaacct aaaacctgtg gaaatggtgt atgccagata tgatggataa aggtgatggc ggttaagccc ttacatcaca gcagcccacg ggcccaggaa ggtcggtggg caaggtcttt aagggggtta 1c- =C.g lfcga gggcatttcg acccaggcat tttgcaaaag caagatctca ggaacttcta tagtgatgac ggtcatgatc atctacaatt ggggccagtg tccctaaaat aa= ata-tgttccaaatct tcttggattg ctggagcttc tcaaaagaat tagaaactgt tggtgagcct tacggcggaa aaccagcgtc agcaattgag tgctccgtca qgaaaatq.t taccattgga tcatcttatg agggttcact cagaaatgct tatcctttgg gcttttggag gtatcctacc tgctg ggatcactga atgttgtcat L Latqg gcacctgtga gtcacaaacc tttcatgtgg gg=Catg=cggc tggaaagtga ggcagaaatc cttgatattg 120 180 240 300 360 420 A sA 540 600 660 705 <210> 520 WO 00/22092PC/Z90I6 PCT/NZ99/00169 <211> 459 <212> DNA <213> Pinus radiata <400> 520 attttgaggt ctgagtgaat tcactgaaaa tggaagggaa ttgtcatcag actttgcgaa gattggttgc atatggacat cctgtgattc agagtttgag acaaaccctc ttgattatgt catgtggagg ctggcaaaga atgcggcctg gagtggcagt tgatttttga cacagagaag tctggcttca catggatggg gaagcatacc ggagccattt caattggcaa gaaacctgga gtgtattagg ggggttatcc gaggcgaaat catttcgttc caggcattct gcaaaagctg gatctcatca acttctata gccagtgagc ctaaaattgc atatgacgga caaatcttac tggattgtca gagcttcagg aaagaatcag aattgaggga cccgtcaatg aaatggtgca cattggagca tcttatggtc gttcactttt aaatgctggc 120 180 240 300 360 420 459 <210> 521 <211> 410 <212> DNA <213> Pinus radiata <400> 521 gggccagtga gcaattgagg ccctaaaatt gccccgtcaa atatatgacg gaaaatggtg tccaaatctt accattggag cttggattgt catcttatgg tggagcttca gggttcactt caaaagaatc agaaatgctg gatcactgaa tgttgtcatc cagattggtt cacctgtgat tcacaaaccc t tcat gtgga gcatgcggcc aatggaaggc agact ttgcg gcatatggac tcagagltttg tcttgattat ggctgccaaa tggagtggca aacacagaga aatctggctt atcatggatg aggaagcata gtggagccat gacaattggc gtgaaacctg agggggttat cagaggcgaa ggcatttcgt cccaggcatt ttgcaaaagc aagatctcat 120 180 240 300 360 410 <210> <211> <212> <213> 522 286
DNA
Eucalyptus grandis <400> 522 atggttcgga tggccaatct ctgtgtggtg attcacagtg aaatcgtaaa gaaggaagtg aaatttcaaa acaaaacaaa tggggtgaca gagttaagta agattatnac tagatggatt aaactagctg agttgcgtaa gtttgcagat tgcagtcatq tttcatgaat ccaagaagat ggcacagaag aatgaggatc ctgttaatgg tgttgctgag tctttaagtt atggcctgaa ggattccttt ctgcaatcct actggatcct ccacacagat t t caga <210> 523 <211> 443 <212> DNA <213> Eucalyptus grandis <400> 523 atggaagccc aagcaagagt tccatcttaa gcactgctgg gccaaagaaa tctggaacgt acccgctttt aqcttccctc gaggggcaac catgctctgt tccgcctaac tgcggatgag ttataaaaaa aaaaaaaaaa C. a agatgatgcc aagtggcaaa agaggggtgc CCttCCCtCC aatttatgct aaataagcat aaa tLttL Ltgtt tacaaggaca tttagcagtg tgtgtaccat tgtaaaacca ggatgtaatg 9ceaLyactt gaagaggatg accgaacaat gaaaagcaat ttactttatla gattcattac atatacagtg ccccagttat gctcaggatg tgctcagtac ttgaaactag t.attcgL -a co acaaatgcac taactttcct <210> 524 <211> 265 wo 00/22092 WO 0022092PCT/NZ99/00 169 <212> DNA <213> Eucalyptus grandis <400> 524 gttaggcatg acgtggtttt ccctgttaga ttttttggcc acgtggaggt tactccaaat ggatcccgaa aatgggtggg tggagaggta ttgcaagctc tagcatatga tgtcccaatt cccggataca agaccaagaa cactaatagt cttcgtctct gggaagcaaa agcttcttct caggatttca accttttcca attcaatgat ggacagtatg aatcagctgg acagttgcac tctcgagctg aacaaatttg tgctg <210> <211> <212> <213> 525 363
DNA
Eucalyptus grandis <400> 525 ggcaattgtc cttgtcataa taacagacgc attaactctg aactttggtt gggaagtcaa cctgaagatt atatcctgca aagttttcca ctctctgttg agagtgcaac tgtccagctg ctg tatcccatgc gaatcgcaag cccactgccg gcgatgcact cat ct cc agg attcacactq ctcgtcccac agtgggatga tgatccttct acagagaaag atataaaaca tccatcattg ccaagtcctt gtgtcattca ccacctttcc aattgttgct gcacaaattt aattqqaaaa catcgtccat gctgtactgc tctccttgaa tcagccgtaa gttcagctcq ggttgaaatc 120 180 240 300 360 363 <210> 526 <211> 344 <212> DNA <213> Eucalyptus grandis <400> 526 ggcaattgac cttgtcataa taacagacgc attaactctg aactttggtt gggaagtcaa cctgaagatt atatcctgca aagttttcca ctctctgttg agagtgcaac tgtccagctg tatcccatgc gaatcgcaag cccactgccg gcgatgcact catctccagg attcacactg ctcgtcccac agtgggatga agaaccttct acagagaaag atataaaaca tccatcattg ccaagtcctt gtgtcattca ccacctttcc aattgttgct gcacaaattt aat t catcgtccat gctgtactgc tctccttgaa tcagccgtaa gttcagctcg <210> <211> <212> <213> 527 445
DNA
Eucalyptus grandis <400> 527 cacagtacat attgcgggtg aagtgaagcg cattcatgaa gatataagaa acttaaggag ttatgatcgg tggaaaggca tgactgatgt tggtaatgtt tatttgttcc aaactacaac tgaatggctg cctcattata acgggtatca tacaagagac atgagtcctg tttgcaacat gtcaacagtg gtctcagtag ggaac gtatagaccc agctgatgaa aagagaggaa atacgaatgc atcctgaggt cagagattct aaacagtcta tattctgggc aaagacaact taaaagaata caacgactac cattcctgga tttgacattc gtgatctata tcacgaacag gtcaagcttg ttgaaggtta agtgagCtat 120 180 240 300 360 A )t 445 <210> 528 <211> 529 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 ':400> 528 cacagtacat attgcgggtg aagtgaagcg cattcatgaa gatataagaa acttaaggag ttatgatcgg tggaaaggca tgactgatgt tggtaatgtt tatttgttcc aaactacaac ctcagcacat cagcactgca tgaatggctg cctcattata ttggggaaca gaatttcttc acgggtatca tacaagagac atgagtcctg tttgcaacat gtcaacagtg gtctcagtag ggaatggagg ggaacattgg ctctttggtg gtatagaccc agctgatgaa aagagaggaa atacgaatgc atcctgaggt cagagattct cgagtggcac atggggccaa cacagctgat aaacagtcta tattctgggc aaagacaact taaaagaata caacgactac cattcctgga aagcaatatg tgtggaaatc gaggtccct t t tga catt c gtgatctata tcacgaacag gtcaagcttg ttgaaggtta agtgagctat aaatttgcac agggaagaaa 120 180 240 300 360 420 480 529 <210> 529 <211> 505 <212> DNA <213> Pinus radiata <400> 529 gccccgtcgg tggttggatc gggcggtgac aaatctacat cgcggacaag ccagacttcc tctggcccat tatgcacgtg tgttcagatc aagcgtatcc ctaccggtac ctacgtttga aacccacatc ttcggtggca gttgatcgtc aacgtcgcca tattattttc tgccctgatt aatgtaaccc ggctcaagga atgccaagtg agacgttggg acgaatataa aatcgtcgtc aggctgctcc aggtgatcaa actcg tgctctctct cttgtacaag gacggcggca agtcgagatt acgccaaacc gcccgaggag cggttactac caacgatccg gacctcatca cttaagggac aagcagaaga gataccaaga atgaacatct cgcaagaaga atggccaagc gaagtcaagg ccaaaactct tcctccctta acaaggagcg gtttgtttga ttggtgtgat ttcaggcccg taactattcg gcttgatgac 120 180 240 300 360 420 480 505 <210> 530 <211> 540 <212> DNA <213> Pinus radiata <400> 530 cggaggtgct ggatgtccta tgaagcgtac tactcatcag tcttatggga tgaagctttg agctcttggt tctggactta gataattgat gttcaatttg natcacgtgg caggccacag tatttcaggg cggtcattat aagcaacttg aatggagggc ccagcctggg gggtttcagc tggatcagta agtatacggt cacatagtgt aaaataatcc caaacagcat gctttgactt ttgctcgact gatatggatt gtgaacaacc tgtgaaaaat tgcgggctcg gcgagaccgt aaagcggttg ttacaatctt ggaaacactg ctcagcatgt gcgttatcag agattattgg gatgttgtga cggttcgact ttaattgaaa tttttcctat ggcataaaag gtggagcagg ctgatggact tatgggttgt ctgaattttg gcattcaaaa ttagcgattt gttggaatga ccctcgagtt accaatatgc aaggagatgc cacttgcaac t taggcaggt gtaatccttg 120 180 240 300 360 420 480 540 <210> 531 <211> 398 <212> DNA <213> Eucalyptus grandis <400> 531 gctggagaac atgtgctgga tgaagangnc cagagaaaag gactgcggac atgtctgagg atcnactcat ggtgatagca ggagacatgg attggtcaac tggccttata cggggtgaaa ggatttggaa ctaagcgtcg acttatctga acagaggcan agaaggggaa acatggagct cctcgcgcgg act tgaacgt 9ggagaaaag nttgcctagg gaagctttac tggccgtg gaggagaagc gaaaggggt c ggtgatgcag ataagttctg cttgtattaa agcttgaagg gcagagaagc tcagcgatat ttgatgcaat taagtcttca WO 00/22092PC/Z9016 PCT/NZ99/00169 <210> 532 <211> 225 <212> DNA <213> Eucalyptus grandis <400> 532 ggagcccgca ggagaggaac acgcgactgg cgcggcagaa gaagcagctt gaaggtgaag aacgtgaaag gggtcgcaga gaagcgactg aaaagggtga tgcagtcagc gatatatcga agaacatgtg ctggaggatt tggaacctcg aagcccagag aaaatctaag cgtcgacttg cggacatgtc tgaggactta tctgagggag ctcatggtga tagca 120 180 225 <210> 533 <211> 415 <212> DNA <213> Eucalyptus grandis <400> 533 taccgctcct gggtcaaggc gagaacatgt gctggaggat gaagcccaga gaaaagctaa gcggacatgt ctgaggactt actcatggtg atagcaacag acatggattg gtcaacagaa cttatacggg gtgaaaacat ccaggcgacq ttggaacctc gcgtcgactt atctgaggga aggc aga tt 9 ggggaagaag ggagcttggc aggagcccgc gcgcggcaga gaacgtgaaa gaaaagggtg cctaggataa ctttaccttg ccgtgactcg aggagaggaa agaagcagct ggggtcgcag atgcagtcag gttctgttga tattaataag gatactggtg cacgcggctg tgaaggtgaa agaagcgact cgatatatcg tgcaatggag tcttcatggc gtcag <210> <211> <212> <213> 534 335
DNA
Eucalyptus grandis <400> 534 ggattccgct gctcttctat acttggtcga gataagttgg caatacgaca tacaagataa tgagatagtg ataactagta ttttgatccg atattggaga tggaaggttc atgccgcgca ctggggctct aacaactcct tgcgccgaat ctaggcagga agaagctacg tggctataat taacgtacca caagcaaggc agaggcagaa gattgatgag agcaaggata acctc atgctattta cgtctgtcca gaactgtccc caatggcgct aagcgcaatg ctggtcactc gggatgaaat ttgattcctc tgtatgatgc taagctgtta <210> 535 <211> 238 <212> DNA <213> Eucalyptus grandis <400> 535 cttccctttc tctctctaga atccttatct cagcgcccct gcctgcggcg gcgatggcgg cgatcctcga cgtcggcccg gcgctcgacg gcggccgctt cagccccacc cgctacttcg cctgcgacgg gaggctcttc tctctctagc ggaacgactg gatcaacagc tacctcgagg acaagaaatc gtcgctgctg ctgcgggagc tcgaggaggt catcaccggc ttcgatga <211> 441 <212> DNA <213> Eucalyptus grandis <400> 536 cttgcttgag gagttgttcc aacttatctc gtacgtttag agccccagat agaagagcag gaccaagtga gtgaccagta aatagcattg cggaatcccc agcatcagca tagtgaccat WO 00/22092PC/Z90I6 PCT/NZ99/00169 ggatggcaac tttgtattac cctttggaat tctctcccat cgccataact aaacgcctgg aggccacact atggctgagt atacttatct ttcgtccgat ccaatcaaca cattgaacct ggttggccac gcaccgtcaa Cttggtccaa aaaccttcag tctggtgatg cgccaact tg caaattcagg aaggtatacg aatctcttgg atatctgtct ctctcctagg gatgtggggc gacgatataa agacaacatc agtaagcaag acttttgaca cataaaagtt gcaccactgc tccgtgggt t tccactcgat 180 240 300 360 420 441 <210> <211> <212> <213> 537 389
DNA
Eucalyptus grandis <400> 537 qgtttggatg ccgccgagat ggcttgtacg acgggttcga ggggtgagtt gctacggacg ttcagcaatg tgaaagtaga agttcagaca agactcctaa tttttctcga atcctcataa aatgtcacaa ccttgctgaa ggtggttacg tctcaagttg gtacatgccg agattcaact caagagacat gccgatgata ggctttcgg agcacaaggc gagaggaagc aggatggtgg gaaagcgaga ttaccgcgca ctggcattgt aagagatcga ttcgggttag tcatacctcc gcgacctcaa tatggtctga cccgtcctga agagcaatgg gcgacggcgt gggaatggac atccttgatt ggtgatgaga ccccaagaaa 120 180 240 300 360 389 <210> 538 <211> 647 <212> DNA <213> Eucalyptus grandis <400> 538 ctcctcccct ctctagccag ctacctcgag gctgcgggag cttcgatgag ggagaggaac gaagcagctt gggt cgcaga tgcagtcagc ttctgttgat attaataagt ttctctctct cgccccctgc gcgatcctcg cgcggccgct accgatctct acgcggctgg gaaggtgaag gaagcgactg gatatatcga gcaatggaga cttcatggcc agaatcctta ctgcggcggc acgtcggccc tcagccccac accgctcctg agaacatgtg aagcccagag cggacatgtc ctcatggtga catggattgg ttatacgggg tctcctgcga ggcgatggcg ggcgctcgac ccgctacttc ggtcaaggcc ctggaggatt aaaagctaag tgaggactta tagcaacaga tcaacagaag tgaaaacatg ggggaggggc gggaacgact gacaagaaat gtcgaggagg caggcgacga tggaacct cg cgtcgacttg tctgagggag ggcagattgc gggaagaagc gagcttg tcttctctct ggatcaacag cgtcgctgct t cat ca ccgg ggagcccgca cgcggcagaa aacgtgaaag aaaagggtga ctaggataag tttaccttgt 120 180 240 300 360 420 480 540 600 647 <210> 539 <211> 340 <212> DNA <213> Eucalyptus grandis <400> 539 ctttctctct ctagaatcct cccctgcctg cggcggcgat ctcgacgtcg gcccggcgct cgcttcagcc ccacccgcta ctrtaror rroorrAA ct-aac tgtgctggag tatctcctgc ggcggggaac cgacgacaag cttcgtcgag 00 e e- 00 r gatttga gacgggaggc gactggatca aaatcgtcgc gaggtcatca tcttctctct acagctacct tgctgctgcg ccggcttcga ctagccagcg cgaggcgatc ggagcgcggc tgagaccgat 120 180 240 340 <210> 540 <211> 320 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00169 <400> 540 ttccctttct ctctctagaa agcgcccctg cctgcggcgg gatcctcgac gtcggcccgg cggccgcttc agccccaccc cgatctctac cgctcctggg cggaggagac gtctcgttcg tccttatctc cgatggcggg cgctcgacga gctacttcgt tcaaaggtga ctgcgacggg gaacgacrgg caagaaatcq cgaggaggt c gt cagatcga aggctcttct atcaacagct tcgctgctgc atcaccggct gagcgcgcgc ctctctagcc acctcgaggc tgcgggagcg tcgatgagac gcgggggcgg <210> 541 <211> 386 <212> DNA <213> Eucalyptus grandis <400> 541 gtccgtatac cttttggacc atccctgaat ttgttgacgg gagcaagttg gcggtggcca gctggggatt ccgctgctct cactcacttg gtcgagataa gaaatcaata cgacatacaa tctctgagat agtgataact aagagataag tgcactcagc accagtgtgg tctatctgg gttggaacaa gataatgcgc atacta tatattccaa catgtaatac cctgttgcca gctcttaacq ctcctcaagc cgaatagagg aggaactttt aaatgtcaaa tccatogtca taccaatgct aaggccgtct cagaagaact atggccccac agtcctagga ctatgctgat at ttact ggt gtccagggat gtcccttgat <210> <211> <212> <213> 542 326
DNA
Eucalyptus grandis <400> 542 ttccctttct ctctctagaa agcgcccctg cctgcggcgg gatcctcgac gtcggcccgg cggccgcttc agccccaccc cgatctctac cgctcctggg ggaggagacg tctcgttcgg tccttatctc cgatggcggg cgctcgacga gctacttcgt tcaaggtgag ttgatt ctgcgacggg gaacgactgg caagaaatcg cgaggaggtc tcagatcgaq aggctcttct atcaacagct tcgctgctgc atcaccggct agcgcgcgcg ctctctagcc acctcgaggc tgcgggagcg tcgatgagac cgggggcggc <210> <211> <212> <213> 543 363
DNA
Eucalyptus grandis <400> 543 ccctctcctc ccctttctct ctctctctag ccagcgcccc acagctacct cgaggcgatc tgctgctgcg ggagcgcggc ccggcttcga tgagaccgat cycaggagag gaacacgcgg aga ctctagaatc ctgcctgcgg ctcgacgtcg cgcttcagcc ctctaccgct ctggagaaca cttatctcct cggcggcgat gcccggcgct ccacccgcta cctgggtcaa tgtgctggag gcgaggggag ggcggggaac cgacgacaag cttcgtcgag ggcccaggcg gatttggaac gggctcttct gactggatca aaatcgtcgc gaggtcatca acgaggagcc ctCgcgcggc elfl- n qA <211>' 558 <212> DNA <213> Eucalyptus grandis <400> 544 ccttcccttt ctctctctag aatccttatc tcctgcgacg ggaggctctt ctctctctag ccagcgcccc tgcctgcggc ggcgatggcg gggaacgact ggatcaacag ctacctcgag WO 00/22092 WO 0022092PCT/NZ99/00 169 gcgatcctcg cgcggccgct ccgatctcta cgcgactgga aaggtgaaga aagcgactgc atatatcgac caatggagac acgtcggccc tcagcccacc ccgctcctgg gaacatgtgc agcccagaga ggacatgtct tcatggtgat atgggttg ggcgct cgac cgctacttcg gtcaaggccc tggaggattt aaatctaagc gaggacttat agcaacagag gacaagaaat t cgaggaggt atgcgacgag ggaacctcgc gtcgacttga ctgagggaga gcagattgcc cgtcgctgct catcaccggc gagcccgcag gcggcagaag acgtgaaagg aaagggtgat taggataagt gctgcgggag ttcgatgaga gagaggaaca aagcagcttg ggt cgcagag gcagtcagcg tctgttgatg 180 240 300 360 420 480 540 558 <210> 545 <211> 414 <212> DNA <213> Eucalyptus grandis <400> 545 cctgtctctc tctgtttctc cggtgggaaa tggcggggaa ggcccggcgc tcgacgaccc agccccaccc gctacttcgt cgctcctggc tccgggccgc aacatgtgct ggaggatatg gctcaacgca tcgctaagcg tctgtttttn cgagtgggtg caagtcgtcq cgagcaggtc ggngacccgg gaatctcgct gcgtcttgaa tctgtctntc aacagctacc tcgctgctgc atcaccggct ancccgcagg cgcaagaaga catgaaagag tgtctctgtg tggaggccat tgagggagag tcgacgagac agaggaacac agcngcttga gtcgtagaga tctctgtgtg cctcgacgtc gggccgcttc cgatctccac caggttggag gggagaggaa ggca 120 180 240 300 360 414 <210> 546 <211> 289 <212> DNA <213> Eucalyptus grandis <400> 546 CCttcccttt ctctctctag aatccttatc ccagcgcccc tgcctgcggc qgcgatggcg gcgatcctcg acgtcggccc ggcgctcgac cgcggccgct tcagcccacc cgctacttcg ccgatctcta ccgctcctgg gtcaaggccc tcctgcgacg gggaacgact gacaagaaat tcgaggaggt aggcgacgag ggaggctctt ctctctctag ggatcaacag ctacctcgag cgtcgctgct gctgcgggag catcaccggc ttcgatgaga gagcccgca 120 180 240 289 <210> 547 <211> 227 <212> DNA <213> Eucalyptus grandis <400> 547 acatgtctga ggacttatct gagggagaaa atggtgatag caacagaggc agattgccta gggttggtca acagnnggga agaagcttta cggggtgaaa acatggagct tggccgagac agggtgatgc agtcagcgat ntatcgactc ggataagttc tgttgatgca atggagacat ccttgtatta ataagtcttc atggcctata tcggatactg gtggtca 120 180 227 <210> 548 <211> 415 <212> DNA S Pinu <400> 548 gtcggttcat ggccccgaat ggtggttata gttgcagacc aagagcctgc agaagctgat ggtaacttat ctcctaaagc cttgccaccc aatcgacaca aacccatgat tcttgcttta tactctagtc aaagcgtttg gagaatgccg cctcccggta ggagaccttg atatggtccg tcacgacctg gccat taaaa tggacttcag cagctttgat aggtgatgcg atcccaaaaa gagctggcaa caatgtagtt caatggtgat ttttttcaca aaaatctcac atttgacact WO 00/22092 WO 0022092PCT/NZ99/00 169 agtaatggga aacagagatg atatagatga aatgtcggga ggtaatgcag ctgttctaac aacggttttg aagctgatag ataagtatga cttgtatggg caagttgctt atcca <210> <211> <212> <213> 549 299
DNA
Pinus radiata <400> 549 caaaggctgg ccaagaggcg tatcgagcgc atgtcggagg atttgtccga gggtgaaaag aagcgcgctt tttcgcggaa cttctccgac aagcgcctgt acattgtgct cataagcttg ttgggtagag attctgatac aggcgggcag gagcaagggc gctgatgttg ttgcaagtct catggtttag gttaaatacg ggagagatgc c tact ccc ag ggtctgatga taagaggtga ttgtagaact cacagaggac ggcaacgccc caataaggga aaacatggaa tgcaagagc 120 180 240 299 <210> 550 <211> 304 <212> DNA <213> Pinus radiata <400> 550 atagaaagct cagacagcga gcaaagcgtg gtgtccattc ttatggtcgt tacatgcctc gaatgtcggt tatacctcct ggaatggact tcagcaatgt tgtagtgcat gacgagaatt cagataatga tttagataat gattctgttc aattgcccga gtctccttta tggggtgaga tcccacggtt tttgaacaat ccctcgaagc ccattatttt ggctcttgct cgacctgatc ccaagaagaa catggccact ttagtcaagg cttttggtga atgtatcaaa ttgaggaagc tggc <210> 551 <211> 464 <212> DNA <213> Pinus radiata <400> 551 gagagccaac tgaaatgctg catacatagt tcgaattccc gccatacatt ccagagtttg attgggagat ctgattggag tgcagatgca ggggatgctg gacaggccat tctctaggcc caaggaagat atcaatgcaa ccttgattcc gctgagcttg acttctgatc tgtggatgca tagatggagc aagacacacc cttgccttct gaaataaact cgtacaagat tggtgacaag aggatgatga aagaaaagta actgggtcat tgtatggcct ttctggtgct agaacaatta catgcgtagg taccaaggca cattctagag tat tcagaaa attcttaata catgtaatcc ctgaacgttc ttgaaacaag attgaagctg aga a agtggagggg gaattgtttg tttccaaagc atggccacta caatggtttt gattgcattc aagaattgtg 120 180 240 300 360 420 464 <210> 552 <211> 312 <212> DNA <213> Eucalyptus grandis <400> 552 ggcaggggtt tatggcttct ggaagtatgt atatcttgag atgttctaca ttcttaaatt agncgacgag ccccattaat ctggtggcat gcattcgcca tgtaaatatt tccgtctttc ttgaataatt ga gaagtttaz ttcaaagctc ccgggaattg caccgaactc actccaatgc gagaggcgtq gtgaaaactg aagggggttg anaagcatga LyaLqauacL agacccggcg ttcccntggc tcacccgggg gcattccgta <210> 553 WO 00/22092 WO 0022092PCT/NZ99/O0I 69 <211> 442 <212> DNA <213> Eucalyptus grandis <400> 553 cgggcacgta atggagagct ccggcttttt atgaagcttt acatttgcca cttgtcatgg atattgatcc atatcatcct gtaaaaggga ccccaatcac aaaggtacac ctggaagatt tctggaagta tgtttcaaag cattcttaaa ttccgggaat ttaccgctat tggacttaca tggtccggca gaccaggctg tggactaaaa tattcagaga ctcgagaggc tg atagcagaca gttgttgagg gagaaattga ctaccctact tctctgctgC ggctgatgac gtgagacccg caaaaggtgt ctatgacgtg acatggggt t ggcagatttc tggacttcag actggcaggg gcgaatcttg ttttgttcag cggccttcca tnaggatatc tttgagcaaa aggatctatg gtttatggct agatgtttta 120 180 240 300 360 420 442 <210> 554 <211> 421 <212> DNA <213> Eucalyptus grandis <400> 554 aaaagatgca tgaactgatg cccaaacaaa tcgggcacgt tttttgttca gccggctttt gcggccttcc aacatttgcc tatcaggata tcatattgat tctttgagca aagtaaaaag agaggatcta tgaaaggtac g aaggaataca aatggagagc tatgaagctt acttgtcatg ccatatcatc gaccccaatc acctggaaga atttggatgg tttaccgcta ttggacttac gtggtccggc ctgaccaggc actggactaa tttattcaga tcaatttcgt tatagcagac agttgttgag agagataatt tgctacccta aatctctgct gaggctgatg tggatggcgg acaaaaggtg gctatgacgt gaacatggtg ctggcagatt gctggacttc acactggcag <210> 555 <211> 448 <212> DNA <213> Pinus radiata <400> 555 tgcattgcgt cgatgctgna accaaaaagc cattctaaag ctgaacagaa gttcttcaat taagggaaat atatactcat acatcaatgc ctgagactac tactgcccaa ctgtgtcctc ccaactacaa tgttaaaatg gcttaggccc tttgcttcaa atttaactcg caagatggtg agcgtcatgg atcagctcca acggtattgc actgcatcca aaacttattc aaaaaaaa aaacgatgat tcttacttgt aaatgtgtaa ggagccacaa ccaggcagtg ccagaatatg aatgctaaga tttgctccta gttcaagtgt gccgactctc tgttgaattt taaaagcacc gnaaactata caaataaaat aatttggagg gtcaacagga tctttctttg gggatcgaaa gtggctttca aacatttcag gagggctgaa 120 180 240 300 360 420 448 <210> 556 <211> 348 <212> DNA <213> Eucalyptus grandis .0j.55 cctgtacgac gaggtcatga gttcgtgctg tcggccgggc gtatgacagc gtccaggagg tggccatccg gagaactttg aggaattgcc aatgctgttg taaaccggac agtgaaatag ggtacaaccc acggttgcat aagacttgaa agacaaccgg gtttggcact ttgaccacta caagagcccc gctgcagtac aggcttccg tatcgaagtt cgtagagaag cacatatgtc tactggttca accgcgaccg gcccttatgc atctcgctgg cagtggggaa gcaagacccc acaacaggtc cactcgggca catttggctg ctcgcttcaa atccttgg WO 00/22092PC/Z9OI6 PCT/NZ99/00169 <210> 557 <221.> 369 <212> DNA <213> Eucalyptus grandis <400> 557 cctgtaacga cgaggtcatg ggttcgtgct gtcggccggg ggtatgacag cgtccaggag ctggccatcc ggagaacttt aaggaattgc caatgctgtt ataaaccgga cagtgaaata gatggaggg aggtacaacc cacggttgca gaagacttga gagacaaccg ggtttggcac gttgaccact ccaagagccc tgctgcagta aaggcttccg gtatcgaagt tcgtagagaa acacatatgt c tact ggtt c cgcccttatg gcagtgggga tacaacaggt gcatttggct atccttggag aaccgcgacc catctcgctg agcaagaccc ccactcgggc gctcgcttca atggttgcca 120 180 240 300 360 369 <210> 558 <211> 470 <212> DNA <213> Eucalyptus grandis <400> 558 cgggcaagga attgccaatg tcaataaacc ggacagtgaa gccagatgga gggtatttcc agttgattgc tttctatgac tcaccgagag tgttgacacc acggaaacac tggctatgat ataagcctac attaattaag ctcatatagt gtgcatggga ctgttggttt atagttgacc aatgaagctt gacaaccaca cgtttcgagg gagatacgtg gtgactacca gcgcactggg ggcactcgta actacacata gttcacttgc tctctatcga gtcttgggtq ctgctattaa ccattggcta tgccaaggaa gaagcatttg tgtcatcctt tggtcactgg tggtgatacg gcatgtcata ggaagcgaag cggttcacct gtcgatgcac gctgctcgct ggagatggtt ggacttggaa gaaattgcat tgggtgaaaa gctgtcaagg acaaggacaa 120 180 240 300 360 420 470 <210> 559 <211> 356 <212> DNA <213> Eucalyptus grandis <400> 559 gtgaacactg gttatgatga aagcctactt tgattaaggt agctacagtg tgcatggtag cttggttggc catatgagcc catactcctg ttggtgctgc aagaaataca aggatgaagc aattcgtgct aactacgacc tgcactgggc tttccatgtg cgttgaagct tgcagtgctg gccatcaaag ataggttatg gccaaggaag cctgaggatq gaatggaatg aaatctatca aagcaaaggc gttcacctaa ttgatgcaac ttaaaaagca caaaatttgc t taagggcga tgttaaagac caagtccaac taggaataac ctggagtcgc tgaatatgag actacc <210> <211> <212> <213> 560 447
DNA
Eucalyptus grandis <400> 560 gttgccagat ggagggtatt CattCaLCcya yagtgttgac aaaaacggaa acactggcta aaggataagc ctacattaat gccaactcat atagtgtgca aagaaccttg gttggccata agtcgccatg tccctgctgg tccaatgaag acccgtttcg tgatgaaata taaggtgact tgggagcgca tgaacctttc cgctgct cttgttcact agggtcttgg cgtgctgcta accaccattg ctgggtgcca catgtgcctg tgctggtcac ttggcatgtc ttaaggaagc gctacggttc aggaagttga aggatgtcaa tggggacttg atatgggntg gaaggctgtc acctaacaag tgcaacaagg agcgcactgg 180 240 300 360 420 447 WO 00/22092 WO 0022092PCTINZ99/001 69 <210> 561 <211> 398 <212> DNA <213> Eucalyptus grandis <400> 561 tttgattaag gtaactacga tgtgcatggt agtgcactgg gccatatgag cctttccatg tgttggtgct gctgttgaag caaggatgaa gctgcagtgc gaaagccctg ccgacgtaca acaatgcctc aatgcccttg ccataggtta gcgccaagga tgcctgagga ctgaatggaa tgaaatctat ccccagagat tggatgtggt tggttcacct agttgatgca tgttaaaaag tgcaaaattt cattaagggt tccagcagat gcctggtc aacaagtcca actaggaata cactggagtc gctgaatatg gaactacctg gctaccagaa acagctacag accttggttg gccatactcc agaagaaata ctggttggga acctctctna <210> 562 <211> 406 <212> DNA <213> Eucalyptus grandis <400> 562 gacacctggg catcctgaga tggccaagga atcgccaatg tttcaacaaa ccggacaatg atgtcaaatg gaaggcattg gaagctgatt gccttttatg attcaccgag aatgttgaga aaatggtaac actggttatg acttcgagac ctgttggttt aaattgtcga cgaatgaagc atgacaacca aacgttttga atgaaattcg gcccggtatt ggctcttgcc ccactacaca ttgttccctc catctccatt gggtctgggt tgctgccatc gaagttacga gagaaacatt tatgccgttc gctgggcact gatggtaaca tggcatgtta aaagaa caggtccact tggctgctcg ttggagatgg ggggacttgg cagagattgc tatgggtgaa <210> <211> <212> <213> 563 413
DNA
Eucalyptus grandis <400> 563 atggaatgcc aagtttgccg gtccattttc aagggtgaac tgagagtcct gctgatgcca agtgctgcct ggtcttcttg taagatgttc ggtgatttcc tagagagcat ggaatgggag tccatactgc gccacgttct agtatgagaa tgcctgctgg ccaggaatct gtggcagtgc aaaagggcac ctatttgcaa ttgtcttcac gaagtacaag ttgggaaaaa ctctcagcaa tgatcttgct cccggaggaa cgggattgcc tgactacatg gaagaagctg gcacttccga tgcctgaatg tcttccaaca cgcaatgtca ctgcacagc anggctgcga cagaactgaa cgtatactcc ccc tcgc ta a tgacactgct ggt tcggtgt ctggtctcat tga 120 180 240 300 360 413 <210> 564 <211> 398 <212> DNA <213> Eucalyptus grandis <400> 564 gccatactcc tgttggtgct dydidyaaaLa tUddyyclLqd ctggttggga gaaagccctq acctctctca acaatgcctc gtgcagatct tgcttcctcc atacccctga ggaacgtaat gcaatgggat tgctctccat gccgttgaag yu-LyudyLyu.
ccgacgtaca aatgcccttg aacatgactc gttagatttg agcccaggac ctgaatggaa LydadcLuLciL ccccagagat tggatgtggt ttctcaaaaa gtgttaggga ttatcccg tgcaaaattt CdL CaadYYYL tccagcagac gcctggtctt gttcggcaat gcatggaatq gctgaatatg ya i: ,;Ly gctaccagaa cttggtggaa ttccaaaagg ggggccatct <210> 565 WO 00/22092 WO 0022092PCT/NZ99/00 169 <211> 376 <212> DNA <213> Eucalyptus grandis <400> 565 gctgaatgga atgccaagtt ctgaagtcca ttttcaaggg actcctgaga gtcctgctga gctaaagtgc tgcctggtct ctgcttaaga tgttcggtga ggtgttagag agcatggaat ctcattccat actgcg tgccgagtat tgaactgcct tgccaccagg tcttggtggc tttccaaaag gggagctatt gagaagaagt gctggttggg aatctctctc agtgctgatc ggcaccccgg tgcaacggga acaaggaaga aaaaagcact agcaatgcct ttgcttcttc aggaacgcaa t tgc cct goa agctgcagaa tccgacgtat gaatgccctc caacatgaca tgtcaggttc cagccctqqt <210> <211> <212> <213> 566 327
DNA
Eucalyptus grandis <400> 566 gccatactcc agaagaaata ctggttggga acctctctca gtgcagatct atacccctga tgttggtgct caaggatgaa gaaagccctg acaatgcctc tgcttcctcc ggaacgtaat gccgttgaag gctgcagtgc ccgacgtaca aatgcccttg aacatgactc gttagat ctgaatggaa tgaaatctat ccccagagat tggatgtggt ttctcaaaaa tgcaaaattt cattaagggc tccagcagac gcctggtctt gttcggcaat gctgaatatg gaactacctg gctaccagaa cttggtggaa ttccaaaagg 120 180 240 300 327 <210> 567 <211> 346 <212> DNA <213> Eucalyptus grandis <400> 567 gacacctggg catcctgaga tggccaagga atcgccaatg tttcaacaaa ccggacaatg atgtcaaatg gaaggcattg gaagctgatt gccttttatg attcaccgag aatgttgaga acttcgagac ctgttggttt aaattgtcga cgaatgaagc atgacaacca aacgttttga gcccggtatt ggctcttgcc ccactacaca ttgttccctc catctccatt gggt ctgggt gaagttacga gagaaacatt tatgccgttc gctgggcact gatggtaaca tggcat caggtccact tggctgctcg ttggagatgg ggggacttgg cagagattgc 120 180 240 300 346 <210> <211> <212> <213> 568 296
DNA
Eucalyptus grandis <400> 568 gacacctggcq catcctgaga tggccaagga atcgccaatg tttcaacaaa ccggacaatg atgtcaaatg gaaggcattg gaagctgatt gccttttatg acttcgaqac ctgttggttt aaattgtcga cgaatgaagc atgacaacca qcccggtatt ggctcttgcc ocactacaca ttgttccctc catcrccatt gaagttacga gagaaacat t tatgccgttc gctgggcact gatggtaaca caggtccact tggctgctcg t tggagatgg ggggacttgg cagaga 120 180 240 <210> 569 <211> 359 <212> DNA <213> Eucalyptus grandis <400> 569 WO 00/22092 WO 0022092PCTINZ99/00 169 cgagaatgtt taacactggt gcctactttg ctacagtgtg tggttggcca tact cctgtt gagaaacgtt tatgatgaaa attaaggtaa catggtagtg tatgagcctt gg tgc tgct g ttgagggtct t tcgtgctgc ctacgaccat cactgggcgc tccatgtgcc ttgaagctga gggttggcat catcaaagaa aggttatggt caaggaagtt tgaggatgtt atggaatgca gttatatggg gcaaaggctg tcacctaaca gatgcaacta aaaaagcact aaatttgctg tgaaaaatgg ttaaagacaa agtccaacag ggaataacct ggagt cgcca aatatgaga 120 180 240 300 359 <210> 570 <211> 394 <212> DNA <213> Eucalyptus grandis <400> 570 aacgtaatgt tagatttggt ctctccatag cccaggactt tgagggcagc gatgaggatc acgattctat tggtcttgga gcttccgtgc tatgccaaac gtgcatacaa ggttgctatt aaaagcttcc caaccttcct gttagggagc atcccgtact tctgcgcttg gaggatgggc attctaatgc gtaaacagga ggaacctcca atggaatggg gtgccacctt ctgaatctgg ccacgcatca tccgcccagc agagaccttc t cga ggccatctgc ctttgtcttc ggtcatctac gccagttgag tgatgggaat cgtcctcgct aatgggattg acagactaca gtcatgaccc cacttggcta gaaactgctg ctctccaggc 120 180 240 300 360 394 <210> <211> <212> <213> 571 349
DNA
Eucalyptus grandis <400> 571 gttatggttc acctaacaag aggaagttga tgcaactagg aggatgttaa aaagcactgg ggaatgcaaa atttgctgaa ctatcattaa gggtgaacta agattccagc agacgctacc tccaacagct aataaccttg agtcgccata tatgagaaga CCtgctggtt agaaacctct acagtgtgca gttggccata ctcctgttgg aatacaagga gggagaaagc ctcaacaatg tggtagtgca tgagcctttc tgctgctgtt tgaagctgca cctgccgacg cctcaatgc ctgggcgcca catgtgcctg gaagctgaat gtgctgaaat tacaccccag 120 180 240 300 349 <210> 572 <211> 388 <212> DNA <213> Eucalyptus grandis <400> 572 tatcgatggc gatacggaaa tgggtggcat gtcatatggg tattaaggaa gcgaaggctg tggctacggt tcacctaaca caaggaagtc gatgcaacaa tgaggatgtc aaagcgcact atqgaatgcc aagtttgccg ttgcattcac tgaaaaacgg tcaaggataa aggccaactc ggaagaacct ggagtcgcca agtatgag cgagagtgtt aaacactggc gcctacatta atatagtgtg tggttggcca tgtccctgct gacacccgt t tatgatgaga attaaggtga catgggagcg tatgaacctt ggcgctgcta tcgagggtct tacgtgctgc ctaccaccat cactgggtgc tccatgtgcc ttgaagctga <210> 573 <211> 14 21 2> DN <213> Eucalyptus grandis <400> 573 cttttatgat gacaaccaca tctccattga tggtaacaca gagattgcat tcaccgagaa tgttgagaaa cgttttgagg gtctgggttg gcatgttata tgggtgaaaa atggtaacac tggttatgat gaaattcgtg ctgccatcaa agaagcaaag gctgttaaag acaagcctac WO 00/22092 WO 0022092PCT/NZ99/001 69 tttgatggca gcacgaccat aggttatggt tcacctaaca agtccaacag ctacagtgtg catggtaagt gcactgggcg ccaaggaagt tgatgcaact aggaataacc ttggttggcc atatagcct ttccatgtgc ctgaggatgt taaaaagcac tg 240 300 342 <210> 574 <211> 526 <212> DNA <213> Pinus radiata <400> 574 cttggtcaag agatacaata qgctgccaaa ggaaagctga gcattcacag aagaatggaa aaggatagac gcaaacagtt cagaatctgg gaattgccaa agccagactc tggagggtat ttgctttcta aggatgttat atacaggtta caactttaat acagtgtaca gttggcctca tgcagttggt cactattgtt ttccaatgag tgatgacaac tact cgt tt tgatgaaatt taagttcact tggaagtgct tccgccttcc ttggcacttg gatcattaca gcctgctcgc cacatctcca gaaggtctgg cgagctgcta actaccattg ttgggtgcaa acgtaccaga ctgagaaaca catattgtat ttgctggaca tagatggtga gatggcacac t tgaggaggc gctatgggtc aggaggttga ggaggt tctagcggct tgttggtgat ctggggcctt cactgagatt tatctgggtg caaatctgtg accaaataag tgcaaccagg <210> <211> <212> <213> 575 295
DNA
Pinus radiata <400> 575 ggaaaatttg agatcctggt gtggtaatca gcagttggtt tggcacttgc tgagaaacat actattgttg atcattacac atattgtatt tccaatgagg cgtgctcgct tgctggacac gatgacaacc acatctccat agatggtgac caatggtacc ctagcagcta gttggtgatg tggggccttg actgagattg attggtcaat gatacaataa gctgccaaat gaaagctgat cattcacaga gaatgtcaat gccagactcc ggagggtatt tgctttctat ggtgt 120 180 240 295 <210> 576 <211> 481 <212> DNA <213> Pinus radiata <400> 576 gcaatctccg ttttggagtg ttcatggaag tggcctcatt gagcagctat tagaatctct attctattgg tctcggagaa ttagggcaat gcccaatgtt cttacaaggt tgctgttctc aactcccaca tcttgccggt tgacaattcc tctggcaaca cgtgaacat t ccttattgtg gcactctctg gatgggccca ttgatgttcc aatagaaaga tcttcaatag agcctgatgt ccatgggagc ctactttctt aagctggtgt ctcatcaacc gtccagctga ggccatcaat agggtgttga cat tcttatg tatctgcaat tgttttcaca tatctatgtn aatagagcac tggaaaggag acttgctctc gaagggaggt ggtagtggtc gggatagccc gactacatga atgacccatg ttggcgagct acagctggag tcccgtcaaa acattatcag tgagcttgag 120 180 240 300 360 420 480 481 <210> 577 <211> 407 <212> DNA <213> Pinus radiata <400> 577 gcaatctccg ttttggagtg cgtgaacatt ccatgggagc tatctgcaat gggatagccc ttcatggaag tggcctcatt ccttattgtg ctactttctt tgttttcaca gactacatga gagcagctat tagaatctct gcactctctg aagctggtgt tatctatgta atgacccatq WO 00/22092PC/Z/O16 PCT/NZ99/00169 attctattgg tctcggagaa gatgggccca ctcatcaacc aatagagcac ttggcgagct ttaagggcaat gcccaatgtt ttgatgttcc gtccagctga tggaaaggag acagctggag cttacaaggt tgctgtt ctc aatagaaaga ggccarcaat acttgctctc tcccgtcaaa aactcccaca tcttgccggt tcttcaatag agggtgttga gaagggg <210> 578 <211> 332 <212> DNA <213> Pinus radiata <400> 578 ctgctcgctt gctggacact catctccata gatggtgaca aggtctggga tggcacacta agctgctatt gaggaggcca taccattggc tatgggtcac gggtgcaaag gaggttgatg ggggccttgg ctgagattgc tctgggtgaa aatctgtgaa caaataaggc caaccaggca aaagctgat t attcacagag gaatggaaat ggatagacca aaacagttac ga gctttctatg gatgttatta a caggt ta tg actttaatta agtgtacatg atgacaacca ctegttttga atgaaattcg agttcactac gaagtgcttt <210> 579 <211> 500 <212> DNA <213> Pinus radiata <400> 579 attccatggg agctatctgc tgctactttc tttgttttca tgaagctggt gttatctatg cactcatcaa ccaatagagc ccgtccagct gatggaaagg gaggccatca atacttgctc agagggtgtt gagaagggag tgtcattctt atgggtagtg gaggaatgaa gggaaagctg aatgggatag cagactacat taatgaccca acttggcgag agacagctgg tctcccgtca gctacattat gttctgagct ccttcatggg gagagcagct tgattctatt ctttagggca agcttacaag aaaactccca cagtgacaat tgagattgct agtggcctca attagaatct ggtctcggag atgcccaatg gttgctgttc catcttgccg tcctctggca gagaaagctg ttccttattg ctgcactctc aagatgggcc ttttgatgtt tcaatagaaa gttcttcaat acaagcctga catctacttt <210> 580 <211> 465 <212> DNA <213> Pinus radiata <400> 580 gttggtttgg cacttgctga attgttgatc attacacata aatgaggcct gctcgcttgc gacaaccaca tctccataga cgttttgaag gtctgggatg gaaattcgag ctgctattga ttatat ccattggcta agtgctttgg tgcaaaggag gaaacatcta ttgtattgtt tggacactgg tggtgacact gcacactatc ggaggccaaa tgggtcacca gttgatgcaa gcggctagat ggtgatggct ggccttggaa gagattgcat tgggtgaaga tctgtgaagg aataaggcaa ccaggcagaa acaataagcc gccaaatgga agctgattgc tcacagagga atggaaatac atagaccaac acagttacag tct gg agactccact gggtatttcc tttctatgat tgttattact aggttatgat tttaattaag tgtacatgga <210> 582 11 494 <212> DNA <213> Pinus radiata <400> 581 ccggtatata ctccggagag tcctgctgat gctactcgaa atctatctca acaatgtttg aatgcacttg tgagggttct tcccggactt ttgggtggaa gtgcagacct tgcatcatcc WO 00/22092 WO 0022092PCT/NZ99/001 69 aacatgacat ctccgttttg ggaagtggcc gctattagaa attggtctcg gcaatgccca aaggtgctgt tattaaaaat gagtgcgtga tcattcctta tctctgcact gagaagatgg atgttttgat tctc gtttggtgac acattccatg ttgtgctact ctctgaagct gcccactcat gttccgtcca ttccagaagg ggagctatct ttctttgttt ggtgttatct caaccaatag gctgatggaa atacccctgc gcaatgggat tcacagacta atgtaatgac agcacttggc aggagacagc tgagcgcaat agcccttcat catgagagca ccatgattct gagctttagg tggagcttac 180 240 300 360 420 480 494 <210> <211> <212> <213> 582 505
DNA
Pinus radiata <400> 582 cagaaggata cccctgctga gctatctgca atgggatagc tttgttttca cagactacat gttatctatg taatgaccca ccaatagagc acttggcgag gatggaaagg agacagctgg atacttgctc tctcccgcaa agaagggagg ctacattatc tgggtagtgg ttctgagctt gcgcaatctc ccttcatgga gagagcagct tgattctatt ctttagggca agcttacaag aaactcccac agtgacaatt gagat cgttttggag agtagcctca attagaatct ggtctcggag atgcccaatg gttgctgttc atcttgccgg cctctggcaa tgcgtgaaca ttccttattg ctgcactctc aagatgggcc ttttgatgtt tcaatagaaa ttcttcaata caagcctgat ttccatggga tgctactttc tgaagctggt cactcatcaa ccgtccagct gaggccatca gagggtgttg qtcattctta 1.20 180 240 300 360 420 480 505 <210> 583 <211> 399 <212> DNA <213> Pinus radiata <400> 583 cagaaggata cccctgctga gctatctgca atgggatagc tttgttttca cagactacat gttatctatg taatgaccca ccaatagagc acttggcgag gatggaaagg agacagctgg atacttgctc tcttccgtca gcgcaatctc ccttcatgga gagagcagct tgattctatt ctttagggca agcttacaag aaaactccca cgttttggag agtggcctca at tagaat ct ggtctcggag atgcccaatg gttgctgttc catcttgcc tgcgtgaaca ttccttattg ctgcactctc aagatgggcc ttttgatgtt tcaatagaaa ttccatggga tgctactttc tgaagctggt cactcatcaa ccgtccagct gaggccatca 120 180 240 300 360 399 <210> <211> <212> <213> 584 472
DNA
Pinus radiata <400> 584 aaatggaggg tatttccaat tgattgcttt ctatgatgac cagaggatgt tattactcgt gaaatacagg ttatgatgaa gaccaacttt aattaagttc attacacytot acatcaaotgg-gttqqcc tcatccgcct atgtgccaaa gggtgcagcg gaggcctgct aaccacatct cccgaaggtc at tcgagct g actactacca a'ct! 7lta tttgaagcaa cgcttgctgg ccatagatgg tgggatggca ctattgagga ttggctatgg agtggtcatc acactggggc tgacactgag cactatctgg ggccaaatct gtcaccaaat taagagrcac taaatttgcc cttggaaagc attgcattca gtgaagaatg gtgaaggata aaggcaaaca tggagtaggc ga <210> 585 <211> 531 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 585 aaaacattgg gcattgccct tcttctccga gaccaataga gcagagattc taatagctgc ggaatgaggc cacttccatg cgcactccaa aacggagatt tttaataatg tcatgttgga accctcacaa gtgcattcaa tccggagtat ataccggcag gccttgaact cagcagcctc aaagctcgcc atcatgggtc tctcatgtag aagggctatc gcgctcagca tgatgcaagg acattgttga agccttatcc ctcgggatat aagtgctcta attacataga tctgtgaaaa ccattgcaga ggggtatcct tctgcacttc gatagatttc cacccttcag ttcttctcat ttggttgatg taacaccagc caaagaaagt tagctcccat tctggaagtc cacccaaaag gagtagcatc gcaatttacc gttcggcaaa agtgggccca ttcagagagt agcacaataa ggaatgttca accaaacatt tccgggaaga agcaggactc actaattagt t <210> <211> <212> <213> 586 385
DNA
Pinus radiata <400> 586 ggcttccgtc agtgggggag gtggaagtca caactggacc gctgagaaac atctagcggc acatattgta ttgttggtga cttgctggac actggggcct atagatggtg acactgagat ggatggcaca ctatctgggt caaaacgcca acttggtcaa tagatacaat tggctgccaa tggaaagctg tgcattcaca gaaga ggacatcctg ggaattgcca aagccagact atggagggta attgctttct gaggatgtta aaaattttga atgcagt tgg ccactattgt tttccaatga atgatgacaa ttactcgttt gactcctggt tttggcactt tgatcattac ggcctgctcg ccacatctcc tgaaggtctg 120 180 240 300 360 385 <210> <211> <212> <213> 587 314
DNA
Eucalyptus grandis <400> 587 ggcacacctt gctgccgctg ccgggaccgg tgtattactg ggactcttac tgggtcatca cggcaaaggc gattgtcaac aatctcatct atggtgcgag ggtctactac acgaaccgga gtgccatcta tgaaaagaca catgacaagg tgaaagagca tgat tcgttattcc ggggcttgtt acctcctana gccaacctcc aatttgtcgt cggtatccgt agcttctaag cacatatggc ccttctgagt gaagt ctctc ttcagggagg atggatgtga catgtcctaa gcaatgattc cctgctcttc <210> 588 <211> 479 <212> DNA <213> Eucalyptus grandis <400> 588 gttaattcaa tgtggaagtc cagcgctatt ccattatccc gaattttatt attgggattc attactgcga aaggaattat gtcagatatt attacgatta ctcgacaaag aatacaaata gctcttaatc gatttttctc tctaggcaaa gcaaaagtat gtattggatc attaaatgct L Lac L LCLd.I.LC tactcaggat ttggatgaaa cgatagtgat caactagtcc ccgtttattg atcaaaggtc cttgatctca aya LfcdU atggaactat acccactcag aaacctcgtc ctgatgttct ttccagggga ttctcacgag ttaaagaata cccrC:aL LaLC taagagaagc ttaacttgcc ctgaatctta tatcaatcca aagatttcat tggaatgaat tggatttatt agtcccaatc tggaggatac cagagaaga <210> 589 <211> 362 <212> DNA WO 00/22092 WO 0022092PCT/NZ99/001 69 <213> Eucalyptus grandis <400> 589 aagacacatg acaaggaatt ttttggacat caggtcttca agtcgttatt acgcaatgtg tctgcttcaa atatatcaag acagctgaat ctggatggga acgttggcaa caacatcaat ct tgtcgtgaag tcagatcacc ggataaaccc cacttctgaa ctttagtacc tttaccggtt tctctccctg gtccaggacc aggcccgaat aagcagaagt agatggatga gatt tgaaca ctcttctgaa gtgactgtag cttcaacaat tctatcggga ggaatcctga tatacatact agagcatgaa tcataatttg cgacaaggag agtagcttcg ggatattact aaggatggaa 120 180 240 300 360 362 <210> <211> <212> <213> 590 190
DNA
Pinus radiata <400> 590 ggacagttac tggataatca ggggtcttct tgccagtaaa atgtatgaaa cagcaaaagg gattgtcaac aatcttgttt cacttataca taaatatgga tttgtcctaa atggtgcacg gacttactat acgaacagaa gtcaacctcc tctcctaagt gccatggtac gggccattta catgaaaaca <210> 591 <211> 301 <212> DNA <213> Pinus radiata <400> 591 ggacagttac tggataatca ggggtcttct gattgtcaac aatcttgttt cacttataca gacttactat acgaacagaa gtcaacctcc catgaaaaca ggggatattg atctattaaa cagattttgg aactcaggaa tccataaagt
C
tgccagtaaa taaatatgga tctcctaagt aatggcattc tatagtacgg atgtatgaaa tttgtcctaa gccatggtac ccaactttgt gatgcgcatg cagcaaaagg atggtgcacg gggccattta tacaggaaca gtgctgaaca 120 180 240 300 301 <210> 592 <211> 468 <212> DNA <213> Eucalyptus grandis <400> 592 cgggcttcct CCtaagccgc gccagcgtcc tCgtcaccgg ctgctcggcg gcatccgggc gtccggagag tcagggagct gaccttcgcg acagaccagc atacacttg Ctggattgaa gataacaatc ttattgggac aagcttgttt tctcgtcatc cttcccgttc cggctgCggg caccgtcgtc cgccggcgag CCtcgaggaa agcagtcggc aattacttta tgcacagttt gggggtcgtc tacattggga gacaacctgg tacggcccta ctattcgcct gaaagtgtac ctagaagtga atggctggcc ggcggcggca gccacaccgt acaactcctc acctcgactt cgacaaagtt agaaaccgct tggcttccca taaggagg tggcggcgca gctccagctc ggaaatcgcc ccacaagatg tgatgctgtc gctttattat tgaatgtaaa 120 180 240 300 360 420 468 <211> 601 <212> DNA <213> Eucalyptus grandis <400> 593 cgggcttcct cctagccgcc ttcccgttcg ggggtcgtcg gcggcggcat ggcggcgcag WO 00/22092 PCT/NZ99/O00169 ccagcgtcct tgctcggcgg tccggagagt gaccttcgcg atacactttg gataacaatc aagcttgttt gaggagtttc atttgtcgtg t cgtcaccggc catccgggcc caggggagct acagaccagc ctggattgaa ttattgggac tctcgtcatc ccctttgtgc atgctaccga ggctgcgggt accgtcgtcg cctcgaggaa agcagtcggc aattacttta tgctacagtt tacgaatcca gcggattctg acattgggag acaacctgga t aegg e ct a ctattcgcct gaaagtgtac ctagaagtga tatggctggc tatggacgaa agtggaaatc ccacaccgtg caactcctcg acctcgactt cgacaaagtt agaaaccgct tggcttccca ctaaggaggt ccaagctaat atattgctga Ctccagctcc gaaatcgccg ccacaagarg tgatgctgtc gctttattat tgaatgtaaa cccatgcacg tatcgaagat gatacttcaa 120 180 240 300 360 420 480 540 600 601 <210> <211> <212> <213> 594 239
DNA
Eucalyptus grandis <400> 594 gattacatcc atgtggttga cttagcagat gaatctaatg tcggttgtga agtgtacaac gagatggttg ctgcatttga gaaggcatct agaagacctg gcgatgcgga aattgtgtat ggaeatatag eagcattacg eaaactcgat ttaggaaeag gtaaaggaac gtcagtnttg ggaaaggnaa ttcctcttgt aatggctgga gcttcgacag agaaagctga gcgcgaatt <210> <211> <212> <213> 595 388
DNA
Eucalyptus grandis <400> 595 ctcctctctc tctctctatc cgccggctac atcggcagec cgtcgtcgac aacctcgaca cggcgagcgc ggccccaacc ggagaaattg ttctcctcga agtaggtgaa agtgtgcaga catcttgctg gaagtcatgg tctaggcgca acacggtgct actcctecc tctccttcca ccaaatttga agccgctgct ctgctcat catggcgaag ccagctcctc cgccgccctc cgaggttgac tgctgtcata ttatttcaac agcgtgctcg ctcggcggcc gaccgggtcc ctccgagaca cactttgctg aataacctca tgacgggcgg accgcgtcgt gcgacctcgc aaccggcgct gattgaaagc atgggaccat <210> 596 <211> 454 <212> DNA <213> Eucalyptus grandis <400> 596 gtttaccggg cagattctga gcacatccta gtggctccat tttgtgcagc aagttgctgt ttcaacgaag gatggtactg acacattqct qcattqcaaa gggaacaggg aagggaactt aaagaaaatt ccactcgtta catcaacaqa aaaqqctqaa atggaagata tggtgaggat cggaagacga gggttcgtga &qCttgaaqa cagtcttgga aggctgggcg cacoiaatta atgttgttga cccaggggga cctgctttga ttacatccat acccggcara gatggttgct ccgaccaggt acto gatacttcaa tcccaaacaa cagtttatng gttgttgatc gggtgtgagg gcatitgaga tgatgctgaa tccagttggt tctaatgcca naacagacta tagccgatgg tgtataatct aggcatctg attgtatatg 120 180 240 300 360 420 A C A <210> 597 <211> 443 <212> DNA <213> Eucalyptus grandis <400> 597 WO 00/22092 WO 0022092PCT/NZ99/00 169 tcgtgacggg 9ccaccgcgt tccgcgacct acaaaccggc ctggattgaa tcaatgggac tttcctcatc ctgtttctgc cggcgccggc cgtcgtcgtc cgccggcgag gctggagaaa agcagtaggt catcatcttg tgctactgtt cacgaatcca tacatcggca gacaacctcg cgcggcccca ttgttctcct gaaagtgtgc ctggaagtca tacggttggc tat gccacacggt acaactcctc acctctcctt cgaccaaatt agaagccgct tggctgctca caaaggaggt gctccagctc ccccgccgcc ccacgaggtt tgatgctgtc gctttatttc tggatgtaag cccatgtaca CtCctcggcg ctcgaccggg gacctccgag atacactttg aacaataacc aagcttgtgt gaggacttcc 120 180 240 300 360 420 443 <210> <211> <212> <213> 598 268
DNA
Eucalyptus grandis <400> 598 tggcgaagag cgtgctcgtg acgggcggcg agctcctcct cggcggccac cgcgtcgtcg ccgccctcga ccgggtccgc gacctcgccg aggttgacct ccgagacaaa ccggcgctgg ctgtcataca ctttgctgga ttgaaagc ccggctacat cggcagccac acggtgctcc tcgtcgacaa cctcgacaac tcctcccccg gcgagcgcgg ccccagcctc tccttccacg agaaattgtt ctcctcgacc aaatttgatq <210> 599 <211> 437 <212> DNA <213> Eucalyptus grandis <400> 599 ctctctctct atctctaggc tacatcggca gccacacggt gacaacctcg acaactcctc gcgcggcccc aacctctcct attgttctcc tcgaccaaat tgaaagtgtg cagaagccgc gctggaagtc atggctgctc ttacggttgg ccaaagg gcacatggcg gctccagctc ccccgccgcc tccacgaggt ttgatgctgt tgctttattt atggatgtaa aagagcgtgc ctcctcggcg ctcgaccggn tgacctccga catacacttt caacaataac gaagcttgtg tcgtgacggg gccaccgcgt tcccgnnacc gacaaaccgg gctggattga ctcaatggga ttttcctcat cggcgccggc cgtcgtcgtC tcgccggcga cgctggagaa aagcagtagg ccatcatctt ctgctactgt 120 180 240 300 360 420 437 <210> <211> <212> <213> 600 578
DNA
Eucalyptus grandis <400.
gtttaccggg gcacatccta tttgtgcagc tcaacgaagg cacattactt gggaacaggg aaagaaaatt tcaacagaaa tgcccgagat actgactgat 600 cagattctga gtggctccat aagttgctgt atggtactgg a-cattgcaaa aagggaactt cactcgttaa aggctgaaca caatggcaact agcttaggat atggaagata tggtgaggat cggaagacga ggttcgtgat ag4-4-gaaga cagtcttgga ggctgggcgc cgaattgaac oggcragcaa tttggcattc atgttgttga cccaggggga CCtgctttga tacatccatg d gatggttgct cgaccaggtg tqqaaqqCca tcgtatta gatacttcaa tcccaaacaa cagtttatgg ttgttgatct 9ggLqtgagg gcatttgaga atgctgaaat aatataoora.
tccagttggt tctaatgcca aacagactat agccgatgga t gtataatct aggcatctgg tgtatatgca tn0 00~ 0 4- CCC C C q 120 180 240 300 360 420 A of) 540 578 <210> <211> <212> <213> 601 160
DNA
Eucalyptus grandis WO 00/22092 PCT/NZ99/0OI 69 <400> 601 gtgatgtt~a ccgggcagat tctgaatgga agataatgtt gttgagatac ttcaatccag ttggtgcaca tcctagtggc tccattggtg aggatcccag ggggatccca aacaatctaa tgccatttgt acagcaagtt gctgtcggaa gacgacctgc <210> 602 <211> 381 <212> DNA <213> Eucalyptus grandis 120 160 <400> 602 gcaataccta atacgagaat catagccata agggttcttg catatttggc cttccagttc cagcatcacc tggtcggcgc caaatgcagc aaccatctcc cacaccctat gccgggttct caacaacatg gatgtaatca gccaaaatcc ctggcccagt aattcgtgtt ccagccttaa aagactgaag tcaagctttt c tcagctatca tccattgatc cagccttttc cgagtggaat ttcccttCCC gcaatgcagc gtcagtcgag tcggcacatc tgttgatgca tttctttcca tgttcccaga aatgtgtcca tcgggggatc tcctcaatgc tatacaattt gatgccttct ttatacacct tcggctagat 120 180 240 300 360 381 <210> 603 <211> 357 <212> DNA <213> Eucalyptus grandis <400> 603 ctctatctct aggcgcacat ggcagccaca cggtgctcca ctcgacaact cctcccccgc cccaacctct CCttccacga tcctcgacca aatttgatgc gtgcagaagc cgctgcttta ggcgaagagc gctcctcctc cgccctcgac ggttgacctc tgtcatacac tttcaacaat gtgctcgtga ggcggccacc cgggtccgcg cgagacaaac tttgctggat aacctcaatg cgggcggcgc gcgtcgtcgt acctcgccgg cggcgctgga tgaaagcagt ggaccatcat cggctacatc cgtcgacaac cgagcgcggc gaaattgttc aggtgaaagt cttgctg <210> <211> <212> <213> 604 315
DNA
Eucalyptus grandis <400> 604 ttgagatact tcaatccagt tggtgcacat gggatcccaa acaatctaat gccatttgtg ttgacagttt atggaacaga ctattcaacg catgttgttg atctagccga tggacacatt atagggtgtg aggtgtataa tctgggaaca gctgcatttg agaag cctagtggct cagcaagttg aaggatagta gctgcattgc gggaagggaa ccattggtga ctgttggaag Ctggggttcg aaaagcttga cttcagtctt ggatcccagg acgacctgct tgattacatc agaacccggc ggagatggtt 120 180 240 300 315 <211> 368 <212> DNA <213> Eucalvntus ornnriiq <400> 605 catgcctggg ttgaaggtta aaggaacgga ggattatatt gatgtcactg atcttgttga acccaagaaa gtgggaatat acaatgttgg tgtggaggct tgcaagaagg caactggagt gcctggtgat tatgcagaag tgtatagtga ctatagcaca tgctcatgtg caccggcaaa ggacatcaaa cccgaccaag ccggatggta aaagcccttg qgtagatcag gtcgattacc gtcaagctcg cttgtgtcag aaaaagcaag tgaaggaatt tgccgcgcag agctaaactg 120 180 240 300 WO 00/22092 WO 0022092PCT/NZ99/001 69 gacggcgaaa tacactgatc ttcaagagag cttgggtatt gcatggagat ggcaaaaggc acaccgca <210> 606 <211> 545 <212> DNA <213> Eucalyptus grandis <400> 606 gcgacctcgc cggcgagcgc aaccggcgct ggagaaattg gattgaaagc agtaggtgaa atgggaccat catcttgctg cctcatctgC tactgtttac tttctgccac gaatccatat tttaccgggc agattctgaa cacatcctag tggctccatt ttgtgcagca agttgctgtc caacg ggccccaacc ttctcctcga agtgtgcaga gaagtcatgg ggttggccaa ggacgaacca tggaagataa ggtgaggatc ggaagacgac tctccttcca ccaaatttga agccgctgct ctgctcatgg aggaggtccc agcttttcat tgttgttgag ccagggggat ctgctttgac cgaggttgac tgctgtcata ttatttcaac atgtaagaag atgtacagag tgaagaaata atacttcaat cccaaacaat agtttatgga ctccgagaca cactttgctg aataacctca cttgtgtttt gacttccctg tgccgtgatg ccagttggtg ctaatgccat acagactatt 120 180 240 300 360 420 480 540 545 <210> 607 <211> 356 <212> DNA <213> Eucalyptus grandis <400> 607 tctagttcic gtcccttcct gcgaagagcg tgctcgtgac ctcctcctcg gcggccaccg gccctcgacc gggtccgcga gttgacctcc gagacaaacc gtcatacact ttgctggatt cccttgctcc gggcggcgcc cgtcgtcgtc cctcgccggc ggcgctggag gaaagcagta tctctctctc ggctacatcg gtcgacaacc gagcgcggcc aaattgttct ggtgaaagtg tctatctcta gcagccacac tcgacaactc ccagcctctc cctcgaccaa tgcagaagcc ggcgcacatg ggtgctccag ctcccccgcc cttccacgag atttgatgct gctgct 120 180 240 300 356 <210> <211> <212> <213> 608 462
DNA
Eucalyptus grandis <400> 608 cgctactgca atcccacacg ctcctctctc tctctctatc cgccggctac atcggcagcc cgtcgtcgac aacctcgaca cggcgagcgc ggccccagcc ggagaaattg ttctcctcga agtaggtgaa agtgtgcaga CatCttgCty gaagtcatgg ccaaattcga tctaggcgca acacggtgct actcctcccc tctccttcca ccaaatttga agccgctgct crgctcatgg cccttctagt catggcgaag ccagctcctc cgccgccctc cgaggttgac tgctgtcata ttatttcaac atgtaagaag tctcgtccct agcgtgctcg Ctcggcggcc gaccgggtcc ctccgagaca cactttgctg aataacctca Ct tcctcccttg tgacgggcgg accgcgtcgt gcgacctcgc aaccggcgct gat tgaaagc atgggaccat 120 180 240 300 360 420 462 <210> 609 <211> 362 <212> DNA <213> Eucalyptus grandis <400> 609 tggcgaagag cgtgctcgtg acgggcggcg gctcctcctc ggcggccacc gcgtcgtcgt cgccctcgac cgggtccgcg acctcgccgg ccggctacat cggcaccaca cggtgctcca cgtcgacaac ctcgacaact cctcccccgc cgagcgcggc cccagcctct ccttccacga WO 00/22092PC/Z90I6 PCT/NZ99/00169 ggttgacctc cgagacaaac cggcgctgga tgtcatacac tttgctggat tgaaagcagt tttcaacaat aacctcaatqg9gaccatcat ta gaaattgttc tcctcgacca aatttgatgc aaggtgaaagt gtgcagaagc cgctgcttta cttgctggaa gtcatggctg ctcatggatg <210> <211> <212> <213> 610 399
DNA
Eucalyptus grandis <400> 610 tggcgaagag agctcctcct ccgccctcga aggttgacct ctgtcataca atttcaacaa gtaagaagct cgtgctcgtg cggcggccac ccgggtccgC ccgagacaaa ctttgctgga taacctcaat tgtgttttcc acgggcggcg cgcgtcgtcg gacctcgccg ccggcgctgg ttgaaagcag gggaccatca tcatctgcta ccggctacat tcgtcgacaa gcgagcgcgg agaaattgtt taggtgaaag tcttgctgga ctgttacgg cggcagccac cctcgacaac ccccagcctc ctcctcgacc tgtgcagaag agtcatggct acggtgctcc tcctcccccg tccttccacg aaatttgatg ccgctgcttt gctcatggat 120 180 240 300 360 399 <210> <211> <212> <213> 611 363
DNA
Eucalyptus grandis <400> 611 ctctctctct atctctaggc tacatcggca gccacacggt gacaacctcg acaactcctc cgcggcccca acctctcctt ttgttctcct cgaccaaatt gaaagtgtgc agaagccgct ctg gcacatggcg gctccagctc ccccgccgcc ccacgaggtt tgatgctgtc gctttatttc aagagcgtgc ctcctcggcg ctcgaccggg gacctccgag atacactttg aacaataacc tcgtgacggg gccaccgcgt tccgcgacct acaaaccggc ctggattgaa tcaatgggac cggcgccggc cgtcgtcgtc cgccggcgag gctggagaaa agcagtaggt catcatcttg <210> 612 <211> 457 <212> DNA <213> Eucalyptus grandis <400> 612 ctctctctct tacatcggca acaacctcga gcggccccaa tgttctcctc aaagtgtgca tggaagtcat acgictggcc atctctaggc gccacacggt caactcctcc CCtCtccttc gaccaaattt gaagccgctg ggctgctcat aaaggaggtc gcacatggcg gctccagtcc cccgccgccc cacgaggttg gatgctgtca ctttatttca ggatgtaaga ccatgtacag aagagcgtgc tcctcggcgg tcgaccgggt acctccgaga tacactttgc acaataacct agcttgtgtt aggactt tcgtgacggg ccaccgcgt c ccgcgacctc caaaccggcg tggat tgaaa caatgggacc ttcctcatct cggcgccggc gtcgtcgtcg gccggcgagc ctggagaaat gcagtaggtg atcatcttgc gctactgttt 120 180 240 300 360 420 457 <210> e& 1 I <22 <213> 613
DNA
Pinus radiata <400> 613 accaaaacca aaattggcgc tggggtcaca agggcaacca gcggaaggct tcaacaagtg cgttctggtg actggaggcg ctggtttcat cggaagccac accgccctgc agctcctcga ggatggctac gaggtttata tcatcgacaa tttagataac tctgttgaag aagcagtgaa 120 180 WO 00/22092 WO 0022092PC'T/NZ99/O01 69 cagagtgagg gatttagttg atcagcgctt ccgcctaaat cttcactttt ttctgggaga tctttgcaac aaagagggat gtagagaagg ttttttcatt ggccaaattc gatgctgtga tacattttgc tggattgaag gctgttggga gaaagtgtag caattccatt acgtaattac caaggaaaaa atctagttgg gca <210> <211> <212> <213> 614 517
DNA
Pinus radiata <400> 614 attcggagaa caaccagagg agccacaccg gataactctg ctaaatcttc tcattggcca gtagcaattc attatggcca caacccaagg tttcgctatt aaggcttcaa ccctgcagct ttgaagaagc acttttttct aattcgatgc cattacgtta aacatggt tg gggattccct gcagaaacca caagtgcgtt cctcgaggat agtccacaca gggagatctt tgtgatacat ttacaagaac caaaaagatg gtggtagaag aaaccaaaat ctggtgactg ggctacgagg gtgagggatt tgcaacaaag tttgctggat aatctagttg gtt tt tt cat actttcc tggcgatggg gaggcgctgg tttatatcat tagttgatca aggatgtaga tgaaggctgt gcactctgaa catcagctac gtcacaaggg tttcatcgga cgacaattta gcgcttccgc gaaggttttt tggagaaagt cctatatgag agtttatggg 120 180 240 300 360 420 480 517 <210> <211> <212> <213> 615 473
DNA
Pinus radiata <400> 615 cattgatatt attttctcac ttcctcacca tgcagatccc gaggcatgtt actctttaga tggagtgcca aggaaagaac ctactttgca gttgctgctg cttcagaaga agctattaca ccttccataa gattgatctt gaatttgatg ctgtcattca aaaaaaaagt ctgaatcaac tttttgaagt attctggtca ggtggttaca agagttgcta ctgggtaaaa ttttgctggg ccgggttatg taatctagaa ttttgggcta ccggaggagc aggttgttgt agctcgctgg gaagctatgg gttaaagctg tcaagaagat tcgtcatttg atacagagcg aggttatgtt aattgataat cgaatatggg agaaattgtt tcggagagag ctgctgttta ggtatttatt atagtgagca ggcagtcaca Ct ggat aac t ggcaatctca cttatcaaca tgt 120 180 240 300 360 420 473 <210> 616 <211> 323 <212> DNA <213> Pinus radiata <400> 616 actttctgct aatgaatcca tgatatttat aaggcagatc tggagctcac ccaagtggcc gcctttcatc caacaagtgg ttaccaaaca aaggatggca tggtcacatt tctgcgcttc tatgggacgc accaagctct cagattggag aataattttg agattggtga agatccaaag ctgtgggaag gcaaccagtg Cagcggttcg agattacatt aga tccttgaaga gattagcccg Ctgaggtact tcaacccagt ggaattccaa ataacctcat ctgaacgtat atggtaatga catgtggtag acttggctga <211> 49-/ <212> DNA <213> Pinus radiata <400> 617 gagaatttcg ctattgcaga aacCaaaacc aaaattggcg atggggtcac aagggcaacc agaggaaggc ttCaaCaagt gcgttctggt gactggaggc gctggtttca tcggaagcca WO 00/22092 WO 0022092PCT/NZ99/00 169 caccgccctg ctctgttgaa tcttcacttt ggccaaattc aattccatta gcc aa cat g aggtgattcc cagctcctcg gaagcagtga tttctgggag gatgctgtga cgttattaca gttgcaaaaa ttgtgta aggatggcta acagagtgag atctttgcaa tacattttgc agaacaatct gatggttttt cgaggtttat ggatttagtt caaagaggat tggattgaag agttggcact tcatcatcag atcatcgaca gatcagcgct gtagagaagg gctgttggag ctgaacctat ctacagttat atttagataa tccgcctaaa ttttttcatt aaagtgtagc atgagattat ggc ca accc a <210> 618 <211> 384 <212> DNA <213> Pinus radiata <400> 618 gagcgatagt gagcatggag atgttggcag tcacactact ataatctgga taactcttca atgggggcaa tctcaccttc tgttcttatc aacagaattt agagtgtagc aaagccactg ttggaaatga ttgatttccc tgccaaggaa t tgcagttgc gaagaagcta cataagattg gatgctgtca ctttactaca caag agaacattct tgctgggtgg ttacaagagt atcttctgga ttcattttgc aaaacaacat ggtcaccgga ttacaaggtt tgctaagctc taaagaagct tgggt tgaaa agttggcacc ggagcaggtt gttgtaattg gctggcgaat atggagaaat gctgtcggag ttaaacttat 120 180 240 300 360 384 <210> <211> <212> <213> 619 354
DNA
Pinus radiata <400> 619 gagcgatagt atgttggcag ataatctgga atgggggcaa tgttcttatc agagtgtagc gagcatggag tcacactact taactcttca tctcaccttc aacagaattt aaagccactg tgccaaggaa ttgcagttgc gaagaagcta cataagattg gatgctgtca ctttactaca agaacattct tgctgggtgg ttacaagagt atcttctgga ttcattttgc aaaacaacat ggtcaccgga ttacaaggtt tgctaagctc taaagaagct tgggttgaaa agttggcacc ggagcaggtt gttgtaattg gctggcgaat atggagaaat gctgtcggag ttaa 120 180 240 300 354 <210> 620 <211> 425 <212> DNA <213> Pinus radiata <400> 620 gaaggcttca acaagtgcgt gccctgcagc tcctcgagga gttgaagaag cagtgaacag cacttttttc tgggagatct aaattcgatg ctgtgataca ccattacgtt attacaagaa aaacatggtt gcaaaaagat gtgat tctggtgact tggctacgag agtgagggat ttgcaacaaa ttttgctgga caatctagtt ggttttttca ggaggcgctg gtttatatca ttagttgatc gaggatgtag t tgaaggctg ggcactctga tcatcagcta gtttcatcgg tcgacaattt agcgcttccg agaaggtttt ttggagaaag acctatatga cagtttatgg aagccacacc agataactct cctaaatctt ttcattggcc tgtagcaatt gattatggcc ccaacccaag 120 180 240 300 360 420 425 <210> 621 <211> 623 <212> DNA <213> Eucalyptus grandis <400> 621 ggaatcagag aaatggctgc acacgcatgg gctctggttc tgggtcttgt tctgatggct WO 00/22092 WO 0022092PCT/NZ99/001 69 tctggggcaa atgcccacat tccttggaca ttcagcatgg cctctcatct tgggcagccg gaggatttat catgtaccag ggaccttggg tgcggatgct tgggggctgc gggctttcga aatacacagg acatcnagtt caaaactcag tacatagtgc ctttggttcg attgtattct gtgaaattcc gggcactcgg tccaagaaag tcacatcaag tactggcttt ggttgctgna agcacgacga agaccaatgt atcccaccgc tcgtggatga ccttcaacca gga cctgtggcgg tacttcaatg caatccaagg agattctgca aatagacttt gttcacgggt cgcctaccac cgtgccgat c gccgatgaaa tggcattcgg gtggctccga ggtcttacct gggacagtca gagttcttgg ggaaaaggag tcctactctg cgggtgttca ttgtactcca tagaaactac gatacagctc gttcgggcat ctgcattcta gtaacaggag acagagaaca ttctctggaa agaacagcaa gcctgtggaa 120 180 240 300 360 420 480 540 600 623 <210> 622 <211> 426 <212> DNA <213> Eucalyptus grandis <400> 622 gcgacttcag agctcgtgaa tcttcttcaa tggctgtccc ggtcttgcgt tgttggtggg ggctgggcta tggaccattt tactctggcg ctgggttcgg atcaagctcg tcgagggcga ggaccgaacc acaacgaatt ctggtc gaaaagcttt ggtcttttcc tctggtcgcg tgtctacgaa gtcgaagagc ctcggctggg cgacttcgag tgctctcgct aaagtgtctg ggtgcgaggt ggagaggttc aagtacatgt accgtcactg ttcctgggca cttcttcttc tgtcgttcgg ttgaggagct tcaagctcaa tcggcaaagt ctttctacat acacgacagg ttcttcttct cctgttcgtg ctaccagccg gcttgacaac taccatccag gtcgtcggat ggagccctac 120 180 240 300 360 420 426 <210> 623 <211> 412 <212> DNA <213> Eucalyptus grandis <400> 623 gcgacttcag agctcgtgaa ttcttcttca atggctgtcc gggtcttgcg ttgttggtgg gggctgggct atggaccatt ctactctggc gctgggttcg gatcaagctc gtcgagggcg tggaccgaac cacaacgaat gaaaagcttt cggtcttttc gtctggtcgc ttgtctacga ggtcgaagag actcggctgg tcgacttcga tgctnctcgc caaagtgtct gggtgcgagg aggagaggtt caagtacatg gaccgtcact gtcctgggca tcttcttctt gtgtcgttcg tttgaggagc ctcaagctca ttcggcaaag gctttctaca aacgacaggg cttcttcttc gcctgttcgt tctaccagcc agct tgacaa ttaccatcca tgtcgtcgga ga 120 180 240 300 360 412 <210> 624 <211> 373 <212> DNA <213> Eucalyptus grandis <400> 624 gcgccaagtc gtgtttcttg ccggggcatc ccgtacccga cgacgactgg gccacccagg agacaacgcc aagcgggca cacggtgtcc gagctgagcc catggtctac gac tggacgagac aggaccagcc gcggccgcat gcagcgccgg tccaccagaa accgatccgc catgggcgtc caagaccgac cagggagagg ccaccagctc gtgcacacca tacagctcga tggacccacg cggtactggt aagtgggtcc acatggcgca tatggaacgc ccccctt cat gggacgcgcc aggcccanca <210> 625 <211> 351 <212> DNA WO 00/22092PC/Z9O16 PCT/NZ99/00169 <213> Eucalyptus grandis <400> 625 ggaaaaggag acagagaaca tcctactctg ttctctggaa cgggtgttca agaacagcaa ttgtactcca gcctgtggaa gactggtcca aggcgccgtt cgtcggttga ggccaagttc gaggatttat catgtaccag ggaccttggg tgcggatgac cgtggcctct tgcgcaactc ct tt ggt tcg attgtattct gtgaaattcc tgggccact c taccgggggt agggccaagg atcccaccgc tcgtggatga ccttcaacca ggggagggct tccacattga tggtgggacc cgcctaccac cgtgccgatc gccgatgaaa tgagaagacc cgggtgcgaa a 120 180 240 300 351 <210> <211> <2 12> <213> 626 270
DNA
Eucalyptus grandis <400> 626 gccgtacata gtgcagacca atgtgttcac ttatctttgg ttcgatccca ccgccgccta ccagattgta ttcttcgtgg atgacgtgcc ttggggtgca attccccttc aaccagcccg gatgactggg ccactcgggg agggcttgag agggaggaaaa ggagacagag aacagaggat ccactcctac tctgttctct ggaacatgta gatccgagtg ttcaagcaca gcaaggaacc atgaaattgt actccagcct gtggaatgcg <210> 627 <211> 267 <212> DNA <213> Eucalyptus grandis <400> 627 gcctgtacta tagtgcagac caatgtgttc atttatcttt ggttcgatcc caccgccgcc taccagattg tattcttcgt ggatgacgtg cttggggtga aattcccctt caaccagccg gatgactggg ccactcggga ggcttga acgggaggaa aaggagacag agaacagagg taccactcct actctgttct ctggaacatg ccgatccgag tgttcaagaa cagcaaggac atgaaattgt actccagcct gtggaatgcg <210> 628 <211> 468 <212> DNA <213> Eucalyptus grandis <400> 628 gagaaacaga gcgacttcag ttcttcttct tcttcttcaa Cctgttcgtg ggtcttgcgt ctaccagccg ggctgggcta gcttgacaac tactctggcg taccatccag atcaagctcg gtC9Lcqqat ggaccgaacc ggagccctac ctggtaaaga agctcgtgaa tggctgtccc tgttggtggg tggaccattt Ctgggttcgg tcgagggcga acaacgaatt ccaacgtgta gaaaagcttt ggtcttttCC tctggtcgcg tgtctacgaa gtcgaagagc ctcggctgg cgacttcgag cgtaaacggg tgctctcgct aaagtgtctg ggtgcgaggt ggagaggttc aagtacatgt accgtnactg ttcctgggca gtgggcaa cttcttcttc tgtcgttcgg ttgaggagct tcaagctcaa t cggcaaagt ctttctacat acacgacagg 120 180 240 300 360 420 468 <~211> 559 <212> DNA <213> Eucalyptus grandis <400> 629 gagaaacaga gcgacttcag agctcgtgaa ttcttcttct tcttcttcaa tggctgtccc gaaaaycttt tgctctcgct cttcttcttc ggtct-tttcc aaagtgtctg tgtcgttcgg WO 00/22092 PTN9/06 PCT/NZ99/00169 cctgttcgtg ctaccagccg gcttgacaac taccatccag gtcgtcggat ggagccctac ggctcggcct caaccgccaa ggtcttgcgt ggctgggcta tactctggcg atcaagctcg ggaccgaacc ctggtacaga ctggttcgac agtcgtgtt tgt tggtggg tggaccattt ctgggt tcgg tcgagggcga acaacgaatt ccaacgtgta ccccaccact tctggtcgcg tgtctacgaa gt cgaagagc ctcggctggg cgacttcgag cgtgaacggg gacttccact ggtgcgaggt ggagaggttc aagtacatgt accgtcactg ttcctgggca gtgggcaacc cctactccgt t tgaggagct tcaagctcaa tcggcaaagt ctttctacat acacgacagg gggagcagaa cctct gg aa c 180 240 300 360 420 480 540 559 <210> 630 <211> 416 <212> DNA <213> Eucalyptus grandis <400> 630 ctcgtgaaaa aagcttttga ctgtcccggt cttttccaaa tggtgggtct ggtcgcgggt accattttgt ctacgaagga ggttcgggtc gaagagcaag agggcgactc ggctgggacc acgaattcga cttcgagttc tctcgatctt gtgtctgtgt gcaaggttta gaggttctca tacatgttcg gtcactgctt ctggggcaac Cttcttcttc cgttcggcct aggagctcta agctcaagct gcaaagttac tctacatgtc acgacagggg ttcttcttct gttcgtgggt ccagccgggc tgacaactac catccagatc gtcggatgga aaccctacct tcttcaatgg Cttgcgttgt tgggctatgg tctggcgctg aagctcatcg ccgaaccaca ggtaca <210> <211> <212> <213> 631 250
DNA
Eucalyptus grandis <400> 631 tggacgagac accgatccgc gtgcacacca aggaccagcc catgggcgtc tacagctcga gcggccgcat caagaccgac tggacccacg agatcgacgc gtgcgagtgc ccggcgacaa gcagcgccgg acatggagca ccggggcatc ccgtacccga.
tatggaacgc cgacgactgg gccacccagg cccccttcat cacgtcctac cgtaacttcg tggcggcggc agacaccgcc aagcggtgca <210> <211> <212> <213> 632 475
DNA
Eucalyptus grandis <400> 632 gagaaacaga gcgacttcag ttcttcttct tcttcttcaa cctgttcgtg ggtcttgcgt ctaccagccg ggctgggcta gcttgacaac tactctggcg Laccatucag aucaagcccg gtcgtcggat ggaccgaacc gatccctacc tggtacagac agctcgtgaa tggctgtccc tgttggtggg tggaccattt ctgggttcgg tcgagggcga acaacgaatt caacgtgtac gaaaagcttt ggtcttttcc tctggtcgcg tgtctacgaa gtcgaagagc ctcggctggg cgacttcgag gtgaacgggg tgctctcgct aaagtgtctg ggtgcgaggt ggagaggttc aagtacatgt accgtcactg ttcctgggaa tgggaaccgg cttcttcttc tgtcgttcgg t tgaggagct tcaagctcaa tcggcaaagt ctttctacat cacgacaggg gacaa <210> 633 <211> 416 <212> DNA <213> Eucalyptus grandis <400> 633 gagcgacttc agagctcgtg aagaaaagct tttgctctcg ctcttcttct tcttcttctt WO 00/22092 WO 0022092PCT/NZ99/001 69 cttcttcttc tgggtcttgc cgggctggge actactctgg agatcangct atggaccgaa aatggctgtc gttgttggtg tatggaccat cgctgggttc cgtcgagggc ccacaacgaa ccggtctttt ggtctggtcg tttgtctacg gggtcgaaga gactcggctg ttcgacttcg ccaaagtgtc cgggtgcgag aaggagaggt gcaagtacat ggaccgtcac agttcctggg tgtgtcgttc gt ttgaggag tctcaagctc gttcggcaaa tgctttctac caacacgaca ggcctgttcg ctctaccagc aagcttgaca gttaccatcc atgtcgtcgg ggggag 120 180 240 300 360 416 <210> 634 <211> 232 <212> DNA <213> Eucalyptus grandis <400> 634 ggaaaaggag acagagaaca gaggatttat tcctactctg ttctctggaa catgtaccag cgggtgttca agaacagcaa ggaccttggg ttgtactcca gcctgtggaa tgcggatgac ctttggttcg atcccaccgc cgcctaccac attgtattct tcgtggatga cgtgccgatc gtgaaattcc ccttcaacca gccgatgaaa ttgggcaatc cggggagggt tt <210> 635 <211> 287 <212> DNA <213> Eucalyptus grandis <400> 635 ccagcctgtg gaatgcggat gactgggcca ccaaggcgcc gttcgtggcc tctgaccggg tgaggccaag tgctgcgcta ctcagggcca cctcgatgcc ttccagtacc ggaggctccg ctactgcgct gatcggnaga ggtaccccgc ctcggggagg ggttccacat gaggtggtgg gtgggtgcgc gatntccccg gcttgagaag accgactggt cgacgggtgc gaacgtcggt gaccagaagg agttccagga tcgagataca ccatctacaa gagtgca 120 180 240 287 <210> <211> <212> <213> 636 240
DNA
Eucalyptus grandis <400> 636 ggacgagaca ggaccagccc cggccgcatc gatcgacgcg ccgatccgcg tgcacaccaa atgggcgtct acagctcgat aagaccgact ggacccacgc tgcgagtgcc cggcgaccat cttggagcac cggggcatcc cgtacccgaa atggaacgcc gacgactggg ccacccaggg ccccttcatc acgtcctacc gtaacttcga ggcggcggca gacaacgcca agcggtgcag 120 180 240 <210> 637 <211> 360 <212> DNA <213> Eucalyptus grandis <400> 637 gcacgacgag atcgacttcg caccaacgtg ttctcgcagg tcccacnaaa or~tttprl- -tcccaaaagc tcatgacgaa ctgttgctga ctaacccgcg gagtgttcaa caacttggga agttcctcgg ggaaagggaa gacctctttt aattcctatt gtcgat cggc caatctctcc cagagaacag tcctctctat tcagattctt gtgcctttcc gggaacccct caatttcacc acgaaactag ggtggacaac caagcaacca acacgctcca tctggttcga aataccgctc agtcccataa acccatgagg 120 240 300 360 <210> 638 <211> 401 <212> DNA WO 00/22092PC/Z90I6 PCT/NZ99/00169 <213> Eucalyptus grandis <400> 638 ctcaagccaa aatataagcc ggtggaggtc ctagtgcttc gcttcgcccg cagctgggaa cgggccaaga tcttcagcgg tcgggcttcc ggtccaagaa gtcgccggga actccgccgg cacgacgaga tcgacttcga aaagtataag ctccatggtg cttctaccag gggtcagctc ggagtacctg caccgtgacc agttcctggg ccaaagtata gtgctcgtga gacttcgacc ctgtcgctgt ttcggccgga gcttactact gaacctgagc gagcaatcat gcttgctgct tgacgtgggg ccctcgacag tcgacatgca tgtcttcgca g gg ccc at ga a gatggctgcc tggcagcgac agtgtcgggg gctcaagctc agggccaact <210> <211> <212> <213> 639 461
DNA
Eucalyptus grandis <400> 639 agaaacagag cgacttcata tcttcttctt cttcaatggc ttcgtgggtc ttgcgttgtt cagccgggct gggcctatgg tgacaactac tctggcgctg catccagatc aagctcgtcg gtcggatgga ccgaaccaca gccctacctg gtacagacca gctcgtgaag tgtcccggtc ggtgggtctg accattttgt ggttcgggtc agggcgactc acgaattcga acgtgtacgt aaaagctttt ttttccaaag gtcgcgggtg ctacgaagga gaagagcaag ggctgggacc cttcgagttc gaacggggtg gctctcgctc tgtctgtgtc cgaggtttga gaggttctca tacatgttcg gtcactgctt ctgggcaaca g ttcttcttct gttcggcctg ggagctctac tgctcaagct gcaaagttac tctacatgtc cgacagggga 120 180 240 300 360 420 461 <210> 640 <211> 458 <212> DNA <213> Eucalyptus grandis <400> 640 attgacttcg agttcctggg ttcactcaag ggaagggcaa aatttccaca catactccgt attcctatta gagttttcaa cccatgaaaa tatactcgag atcaagacag actggtcgaa gcctgcacct ggtccacggg ggatccacat ggaaaatcaa gaacctgagc cagggagcag catctggaag gaatggagag cctctggaat atcgcccttc ctcgtcctcc tgagctcgat ggcgaccctt cagttctacc ccccagcaca tcaattggcg gccgatgatt acggcatact tgtgagatcg gc c tatgg acatcctcca tgtggtttga tcatcttctt tgcccttccc gggccacgag acaggaagtt gacggcccgc caccaacgtc ccccaccaga ggtagacaac caagaaccag aggcggactg ccaggccact ttcctactct 120 180 240 300 360 420 458 <210> <211> <212> <213> 641 283
DNA
Eucalyptus grandis ctctggttcg acccaactgc tgatttccac atcatattct cagtggacgg gactcccatc qttcccttcc ccaaggccca occcatopoo tgggcgacca gaggcggg ct cgtgaagacg taccggaact tcaacgccga taacgcctgc acctactcca agagagttca g act gga ca c gtttggtcat tcctctggaa tccacaacgc agaacgcaga gtccatcggt aagcgccctt cactgcttcc ctg <210> 642 <211> 385 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCTINZ99/00I 69 <400> 642 gttcggccgg atcgaagtca aactcacgat gagattgact ccacaccaac gtcttcactc tgaccccacc agaaatttcc cttggtagac aacatcaccc tataagccaa agtataagcc agtgcttcct ccatggtggt atctcaccgt tcgagttcct aagggaaggg acacatactc atctctctct aaagtataga gctcg gaccgcttac ggggaacctg caacagggag cgtcatctgg ctctctcccc gcaatcatgg racttgtctt agcggcgacc cagcagttct aagccccagc cactctctct cccatgaagg cgcaagggcc cttacatcct acctgtggtt acatcatctt caagccaaaa tggaggtcct 120 180 240 300 360 385 <210> 643 <211> 378 <212> DNA <213> Eucalyptus grandis <400> 643 ggcacaccca ttttggccaa gtcctttcga ataagatggc gcggcctccg gcaacttcaa atacccagca gtggccagct cggtccaaga agcagtactt aactccgccg gcaccgtcac atcgacttcg agtttctc ttcctctcct catggcactg caaggacttc cctcacgctg gttcgggaag cgcctattac taatttcaaa gttgcccttg gacatcacgt tccctggaca attgacatgc Ctttcttctt angccatggc gcctcttggt ggggtgatgg agacgtcggg agctcaaact tgggttctac ttttcaaggc ggcggccgcg ccgtgcgcag gt cgggc tt c cgtgcctggg gcacgacgaa <210> <211> <212> <213> 644 430
DNA
Eucalyptus grandis <400> 644 gcgacttcag atctcgtgaa tcaatggctg tcccggtctt gcgttgttgg tgggtctggt gctatggacc attttgtcta ggcgctgggt tcgggtcgaa ctcgtcgagg gcgactcggc aaccacaacg aattcgactt cagaccaacg gaaaagcttt ttccaaagtg cgcgggtgcg cgaaggagag gagcaagtac tgggaccgtc cgagttcctg tgctctcgct tctgtgtcgt aggtttgagg gttctcaagc atgttcggca actgccttct ggcaacacga cttcttcttc tcggcctgtt agctctacca tcaagcttga aagttaccat acatgtcgtc caggggagcc ttcttcttct cgtgggtctt gccgggctgg caactactct ccagatcaag ggatggaccg ctacctggta <210> <211> <212> <213> 645 471
DNA
Eucalyptus grandis <400> 645 ctcaagccaa aatataagcc qgtggaq9tC ctagtgctc gCttCgcccg cagctgggaa cgcgccaaga tcttcagcgg tCoaacttCCi o~rnAA qtcgccga actccgccgg cacgatgaga ttgacttcga accaacgtct tcactcaagg aaagtataag ctccatggtg cttctaccag gggtcagctc caccgtgacc gttcctgggg gaagggcaac ccaaagtata gtgctcgtga gacttcgacc ctgtcgctgt 1 trc~rr gvr qCttactact aacctgagcg agggagcagc gagcaatcat gcttgctgct tgacgtgggg ccctcgacag tgtctcgca gcgacctta agttctacct ggcccatgaa gatggctgcc tggcagcgac agtgtcgggg agggccaacr catcctccac <210> 646 <211> 480 <212> DNA WO 00/22092 WO 0022092PCT/NZ99/00I 69 <213> Eucalyptus grandis <400> 646 aatggctgca cacgcatggg gggggctgct ccaagaaagc ggctttcgat cacatcaagt atacacaggt actggctttc catcaagttg gttgctggag aaactcagag cacgacgaaa catagtgcag accaatgtgt ttggttcgat cccaccgncg ctctggttct ctgtggcggt acttcaatgg aatccaaggg attctgcagg tagactttga tcacgggagg cctaccactc gggtcttgtt ggcattcggt tggctccgag gtcttacctg gacagtcact gttcttgggt aaaaggagac ctactctgtt ctgatggctt agaaactaca atacagctct ttcgggcatt gcattctacc aacaggagtg agagaacaga ctctggaaca ctggggcaat tgcccacatg ccttggacaa tcagcatgga tctcatctca ggcagccgta ggatttatct tgtaccagat 120 180 240 300 360 420 480 <210> 647 <211> 284 <212> DNA <213> Eucalyptus grandis <400> 647 ctgttcgtgg gtcttgcgtt gttggtgggt taccagccgg gctgggctat ggaccatttt cttgacaact actctggcgc tgggttcggg accatccaga tcaagctcgt cgagggcgac tcgtcggatg gaccgaacca caacgaattc ctggtcgcgg gtctacgaag tcgaagagca tcggctggga gacttcgagt gtgcgaggtt tgaggagctc gagaggttct caagctcaag agtacatgtt cggcaaagtt ccgtcactgc cttctacatg tcct 120 180 240 284 <210> 648 <211> 459 <212> DNA <213> Eucalyptus grandis <400> 648 cctcactctc atatcatctc cagcagacag aacacgccca tctgcttctc gtggtcgttt cttcgacata acctggggtg tctctccctc gacaaggcct caagattgac atgcagctca ctatttatct tcaaacgggt gagcggcgat ccatacattc attgagattt atatggcctc cttgggggac atggccgagc ccggctccgg agctagttcc cggcgtggga tccacaccaa gcttctcttc cctttctact gtttgcttcc tcagatcctc cttccagtcc cggcaactcc tgagatagac cgtcttcag agcaactaca tcttcgcttc gcccgcaact aacaacggcg aagaacgagt gctggcaccg ttcgagttct gcagcagcag gcatcgccac tctatcaaga acctcctcac act tgt tcgg tcaccgcata tggggaactt 120 180 240 300 360 420 459 <210> <211> <212> <213> 649 402
DNA
Eucalyptus grandis <400> 649 gcgacttcag agctcgtgaa tcaatggctg tcccggtctt gcgttgttgg tgggtctggt gctatggacc attttgtcta flflrnrt-gr t I- nnnnrnran CtCqtCgaqq gcgactcggc aaccacaacg aattcgactt gaaaagcttt ttccaaagtg cgcgggtgcg cgaaggagag tgggaccgc cgagttcctg tgctctcgct tctgtgtcgt aggtttgagg gttctcaagc actgcctcc ggcaacacga cttcttcttc tcggcctgtt agctctacca tcaagcttga acargtcgtc ca ttcttcttct cgtgggtctt gccgggctgg caactactct ggacggaccg 120 180 240 360 402 <210> 650 <211> 469 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 650 gagaaacaga gcgacttcaq, ttcttcttct tcaatggctg cgtgggtctt gcgttgttgg gccgggctgg gctatggacc caactactct ggcgctgggt ccagatcaag ctcgtcgagg ggatggaccg aaccacaacg ctacctggta cagaccaacg agctcgtgaa tcccggtctt tgggtctggt attttgtcta tcgggtcgaa gcgactcggc aattcgactt tgtacgtgaa gaaaagcttt ttccaaagtg cgcgggtgcg cgaaggagag gagcaagtac tgggaccgtc cgagttcctg cggggtgggc tgctctcgct tctgtgtcgt aggtttgagg gttctcaagc atgttcggca actgccttct ggcaacacga aaccgggag crtcttcttc tcggcctgtt agctctacca tcaagcttga aagttaccat acatgtcgtc caggggagcc 120 180 240 300 360 420 469 <210> 651 <211> 473 <212> DNA <213> Eucalyptus grandis <400> 651 gagaaacaga gcgacttcag ttcttcttct tcttcaatgg gttcgtgggt cttgcgttgt ccagccgggc tgggctatgg tgacaactac tctggcgctg catccagatc aagctcgtcg gtcggatgga ccgaaccaca gccctacctg gtacagacca agctcgtgaa ctgtcccggt tggtgggt Ct accattttgt ggttcgggt c agggcgactc acgaattcga acgtgtacgt gaaaagcttt cttttccaaa ggtcgcgggt ctacgaagga gaagagcaag ggctgggacc cttcgagttc gaacggggtg tgctctcgct gtgtctgtgt gcgaggtttg gaggttctca tacatgttcg gtcactgctt ctgggcaaca ggcaaccggg cttcttcttc cgttcggcct aggagctcta agctcaagct gcaaagttac tctacatgtc cgacagggga agc 120 180 240 300 360 420 473 <210> 652 <211> 454 <212> DNA <213> Eucalyptus grandis <400> 652 gagaaacaga gcgacttcag ttcttcttct tcttcaatgg gttcgcgggt cttgcgttgt ccagccgggc tgggctatgg tgacaactac tctggcgctg catccagatc aagctcgtcg gtcggatgga ccgaaccaca gccctacctg gtacagacca agctcgtgaa ctgtcccggt tggtgggtct accattttgt ggttcgggtc agggcgactc acgaattcga acgtgtacgt gaaaagcttt cttttccaaa ggtcgcgggt ctacgaagga gaagagcaag ggctgggacc cttcgagttc gaac tgctctcgct gtgtctgtgt gcgaggtttg gaggttctca tacatgttcg gtcactgctt ctgggcaaca cttcttcttC cgttcggcct aggagctcta agctcaagct gcaaagttac tctacatgtc cgacagggga 120 180 240 300 360 420 454 <210> <211> <212> <213> 653 435
DNA
Eucalyptus grandis <40>65, gtttgaggag ctctaccagc tctcaagctc aagcttgaca gttcggcaaa gttaccatcc ctgctttcta cThtgtcgtcg gcaacacgac aggggagccc accgggagca gaggctcggc tcctctggaa ccagcgccaa ccaacttgga gcacc cgggctgggc actactctgg aQatcaaact tacctggtac Ctctggttcg gtcgtgtttc tatggaccat cgctgggttc catcaaaao acczacaacga agaccaacgc accccaccac ttgtggacga tttgtctacg gggtcgaaga eni attCgacttC 9tacgtgaac tgacttccac gacaccgatc aaggagaggt gcaagtacat gagutcccgg ggggtgggca tcctactccg cgcgtgcaca 120 240 300 360 420 435 <210> 654 WO 00/22092 WO 0022092PCT/NZ99/00I 69 <211> 386 <212> DNA <213> Eucalyptus grandis <400> 654 ctcaagccaa aatataagcc ggtggaggtc ctagtgcttC gcttcgcccg cagctgggaa cgggccaaga tcttcagcgg tcgggcttcc ggtccaagaa gtcgccggga actccgccgg cacgacgaga tcgacttcga aaagtataag ctccatggtg cttctaccag gggtcagctc ggagtacctg caccgtgacc gttcct Ccaaagtata gtgctcgtga gacttcgacc ctgtcgctgt ttcggccgga gcttactact gagcaatcat gcttgctgct tgacgtgggg ccctcgacag tcgacatgca tgtcttcgca ggcccatgaa gatggctgcc tggcagcgac agtgtcgggg gctcaagctc agggccaact 120 180 240 300 360 386 <210> <211> <212> <213> 655 289
DNA
Eucalyptus grandis <400> 655 ggaacttcta ccaggacttc gacctgacgt gcgggggtca gctcctgtcg ctgtccctcq agaaggagta cctgttcggc cggatcgaca ccggctccgt. gaccgcttac tacttgtctt tcgagttcct. ggggaacctg agcggcgacc ggggtggcag cgaccgggcc aagatcttca acagagtgtc ggggtcgggc ttccggtcca tgcagctcan gctcgtcgcc gggaactccg cgcaagggcc aactcacgac gagatcgact cttacatcct ccacaccan 120 180 240 289 <210> <211> <212> <213> 656 422
DNA
Eucalyptus grandis <400> 656 ctctctcccc cactctctct gcaatcatgg cccatgaagg ttgctgctga tggctgccgc acgtggggtg gcagcgaccg ctcgacagag tgtcggggtc gacatgcagc tcaagctcgt.
tcttcgcaag ggccaactca ga caagccaaaa tggaggtcct ttcgcccgca cgccaagatc gggcttccgg cgccgggaac cgatgagatt tataagccaa agtgcttcct qctgggaact ttcagcgggg tccaagaagg tccgccggct gacttcgagt.
agtataagcc ccatggtggt tctaccagga gtcagctcct agtacctgtt ccgtgaccgc tcctggggaa aaagtataga gctcgtgagc cttcgacctg gtcgctgtcc cggccggatc ttactacttg cctgagcggc 120 180 240 300 360 420 422 <210> 657 <211> 445 <212> DNA <213> Eucalyptus grandis <400> 657 gaaccacaac gaattcgact acagaccaac gtgtacgtga cgaccccacc actgacttcc ccCgaaqqaC Caeccatgy ccagggcggc cgcatcaaga cttcaiagatc gacgcgtgcg gtgcagcagc gccggcaggg tcgagttcct acggggtggg actcctactc ycgtctacag ccgactggac agtgcccggc agagg gggcaacacg caaccgggag cgtcctctgg ctCqataLqq ccacgccccc gaccatggcg acaggggagc cagaggctcg aaccagcgcc aacgccgacg ttcatcacgt gcggcagaca cctacctggt gcctctggtt.
aagtcgtgtt cctaccgtaa acgccaagcg <210> 658 <211> 310 WO 00/22092PC/Z90I6 PCT/NZ99/00169 <212> DNA <213> Eucalyptus grandis <400> 658 ggatttatct ttggttcgat cccaccgccg tgtaccagat tgtattcttc gtggatgacg accttggggt gaaattcccc ttcaaccagc cggatgactq ggccactcgg ggagggcttg tggcctctta ccgggggttc cacattgacg gcgctactca cctaccactc tgccgatccg cgatgaaat t agaagaccga ggtgcgaagc ctactctgtt ggtgt tcaag gtactccagc ctggtccaag gt cggttgag ctctggaaca aacagcaagg ctgtggaatg gcgccgttcg gccaagttct 120 180 240 300 310 <210> <211> <212> <213> 659 482
DNA
Eucalyptus grandis <400> 659 aaacagagcg acttcagagc ttcttcttca atggctgtcc gggtcttgcg ttgttggtgg gggctgggct atggaccatt ctactctggc gctgggttcg gatcaagctc gtcgagggcg tggaccgaac cacaacgaat cctggtacag accaacgtgt ct tcgtgaagaa cggtcttttc gtctggtcgc ttgtctacga ggtcgaagag actcggctgg tcgacttcga acgtgaacgg aagcttttgc caaagtgtct gggtgcgagg aggagaggt t caagtacatg gaccgtcact gttcctgggc ggtgggcaac tctcgctctt gtgtcgttcg tttgaggagc ctcaagctca ttcggcaaag gccttctaca aacacgacag cgggagcaga Cttcttcttc gcctgttcgt tctaccagcc agcttgacaa ttaccatcca tgtcgtcgga gggagcccta ggctcggcct 120 180 240 300 360 420 480 482 <210> 660 <211> 415 <212> DNA <213> Eucalyptus grandis <400> 660 gagaaacaga gcgacttcag ttcttcttct tcttcaatgg gttcgtgggt cttgcgttgt ccagccgggc tgggctatgg tgacaactac tctggcgctg catccagatc aagctcgtcg gtcggatgga ccgaaccaca agctcgtgaa ctgtcccggt tggtgggtct accattttgt ggttcgggtc agggcgactc acgaattcga gaaaagcttt cttttccaaa ggtcgcgggt ctacgaagga gaagagcaag ggctgggacc cttcgagttc tgctctcgct gtgtctgtgt gcgaggtttg gaggttctca tacatgttcg gtcactgctt ctgggcaaca cttcttcttc cgttcggcct aggagctcta agctcaagct gcaaagttac tctacatgtc cgaca <210> 661 <211> 542 <212> DNA <213> Eucalyptus grandis <400> 661 ctctctcaag tgaaggtgga cgaccgggcc ggggt cgggc gctcgtcgcc aactcacgac ccacaccaac tgaccccacc ct ccaaaatata ggtcctagtg aagatctca ttccggtcca gggaactccg gagatcgact gtcttcactc aggaatttcc agccaaagta cttcctccat 9Cgggggtca agaaggagta ccggcaccgt tcgagttcct aagggaagg acacatactc taagccaaag ggtggtgctc gctcccytcg cctgttcggc gaccgcttac ggggaacctg caacagggag cgtcatctgg tatagagcaa gtgagcttgc cggatcgaca tacttgtctt agcggcgacc cagcagttct aagccccagc tcatggccca tgctgatggc acaya9tgtC tgcagctcaa cgcaagggcc cttacatcct acctgtggtt acatcatctt WO 00/22092 WO 0022092PCT/NZ99/00 169 <210> 662 <211> 300 <212> DNA <213> Eucalyptus grandis <400> 662 gtcgcgggtg cgaggtttga ggagctctac tacgaaggag aggttctcaa gctcaagctt aagagcaagt acatgttcgg caaagttacc gctgggaccg tcactgcttt ctacatgtcg ttcgagttcc tgggcaacac gacaggggag cagccgggct gacaactact atccagatca tcggatggac ccctacctgg gggctatgga ctggcgctgg agct cgtcga cgaaccacaa tacagaccaa ccattttgtc gttcgggtcg gggcgactcg cgaattcgac cgtgtacgtg 120 180 240 300 <210> 663 <211> 424 <212> DNA <213> Eucalyptus grandis <400> 663 tcaagctcaa gcttgacaac tcggcaaagt taccatccag ctttctacat gtcgtcggat acacgacagg ggagccctac gggagcagag gctcggcctc tctggaacca gcgccaagtc acttggagca ccggggcatc tatg tactctggcg atcaagctcg ggaccgaacc ctggtacaga tggttcgacc gtgtttcttg ccgtacccga ctgggttcgg tcgagggcga acaacgaatt ccaacgtgta ccaccactga tggacgagac aggaccagcc gtcgaagagc ctcggctggg cgacttcgag cgtgaacggg cttccactcc accgatccgc catgggcgtc aagtacatgt accgtcactq ttcctgggca gtgggcaacc tactccgtcc gtgcacacca tacagctcga <210> 664 <211> 456 <212> DNA <213> Eucalyptus grandis <400> 664 cagcagcagc agcaacagaa tctttgtacc gccgcgcttc ccgcaacttt tatcaagact caatggcgac ctcctcactc gaacgagtac ttgttcggca aggcactgtc accgcatact cgagttcttg gggaacttga aggcaagggc aaccgagagc aacaaaacgc ttcttatcat tcgacataac tctcccttga agattgacat atttatcttc gcggcgatcc agcaattcta gcccaatatg ggtcgtttct ctggggagat caaggcctcc gcagctcaag aaatgggtca ctacattctt tctctg gcctctcggt tgggcaacat ggccgagctc ggctccggct ctcgttcctg acgtgggacg cacaccaacg ccacttcttc ccgcttccgC agatcctcaa tccagtccaa gcaactccgc agatagactt tgttcagcca <210> <211> <212> 665 420
DNA
Eucalyptus grandis <400> 665 coaccattoc acattctcaa~ catcggcgtt cccttcocca ggacgactgg gcaaccagag tgcttcctac aggaacttca ttgcacttcg tcttcatctt aacaagccaa gagaggctga agatgccaaa agattccccc toAataoA r aggcccagcc gcgggctcgt atgctgataa cttcggatgg agtgggttca aaggcgtccc t ~rroat R~O A catgaggata aaagacagat cgcCtgcgtt taatgcatgg gagcaactac tcctgagtgc ttctcgagcc tggacacaag tcgtcatctg ctatcggaag atgatctaca accatgtcct tctggaacgc cgcccttcac qgtcctcatc agctcgactc actactgtac agacgacaca 120 180 240 300 360 420 WO 00/22092PCIZ9016 PCT/NZ99/00169 <210> 666 <211> 434 <212> DNA <213> Eucalyptus grandis <400> 666 aacagagcga cttcagagct tcttcttcaa tggctgtccc ggtcttgcgt tgttggtggg ggctgggcta tggaccattt tactctggcg ctgggttcgg atcaagctcg tcgagggcga ggaccgaacc acaacgaatt ctggtacaga ccaa cgtgaagaaa ggtcttttcc tctggtcgcg tgtctacgaa gtcgaagagc ctcggctggg cgacttcgag agcttttgct aaagtgtctg ggtgcgaggt ggagaggtt c aagtacatgt accgtcactg ttcctgggca ctcgctcttc tgtcgttcgg ttgaggagct tcaagctcaa tcggcaaagt ccttctacat acacgacagg ttcttcttct Cctgttcgtg ctaccagccg gcttgacaac taccatccag gtcgtcggat ggagccctac 120 180 240 300 360 420 434 <210> <211> <212> <213> 667 464
DNA
Eucalyptus grandis <400> 667 gcgacttcag agctcgtgaa tcaatggctg tcccggtctt gcgttgttgg tgggtctggt gctatggacc attttgtcta ggcgctgggt tcgggtcgaa ctcgtcgagg gcgactcggc aaccacaacg aattcgactt cagaccaacg tgtacgtgaa gaaaagcttt ttccaaagtg cgcgggtgcg cgaaggagag gagcaagtac tgggaccgtc cgagttcctg cggggtgggc tgctctcgct tctgtgtcgt aggtttgagg gttctcaagc atgttcggca actgccttct ggcaacacga aaccgggagc cttcttcttC tcggcctgtt agctctacca tcaagcttga aagttaccat acatgtcgtc caggggagcc agag ttcttcttct cgtgggtctt gccgggctgg caactactct ccagatcaag ggatggaccg ctacctggta <210> <211> <212> <213> 668 457
DNA
Eucalyptus grandis <400> 668 tctctctctc ccccactctc agagcaatca tggcccatga agcttgctgc tgatggctgc ctgacgtggg gtggcagcga tccctcgaca gagtgtcggg atcgacatgc agctcaagct ttgtcttcgc aagggccaac ggcgaccctt acatcctcca tctcaagcca aggtggaggt cgcttcgccc ccgcgccaag gtcgggcttc cgtcgccggg tcacgatgag caccaacgtc aaatataagc cctagtgctt gcagctggga atcttcagcg cggtccaaga aactccgccg attgacttcg ttcactc caaagtataa cctccatggt acttctacca ggggtcagct aggagtacct gcaccgtgac agttcctggg gccaaagtat ggtgctcgtg ggacttcgac cctgtcgctg gttcggccgg cgcttactac gaacctgagc 120 180 240 300 360 420 457 <210> 669 <211> 434 <212> DNA <213> Eucalyptus grandis <400> 669 ggctgcacac gcatgggctc ggctgctcca agaaagcctg tttcgatcac atcaagtact cacaggtact ggctttcaat caagttggtt gctggagatt ctcagagcac gacgaaatag tggttctggg tggcggtggc tcaatggtgg ccaaggggtc ctgcagggac actttgagtt tcttgttctg attcggtaga ctccgagata ttacctgttc agtcactgca cttgggtaac atggcttctg aactacatgc cagctctcct gggcatttca ttctacctct aggagtgggc gggcaatggg ccacatgggc tggacaaata gcatggacat catctcaaaa agccgtacat 120 180 240 300 360 WO 00/22092 PCT/NZ99/00 169 agtgcagacc aatgtgttca cgggaggaaa aggagacaga gaacagagga tttatctttg gttcgatccc accg <210> 670 <211> 294 <212> DNA <213> Eucalyptus grandis 420 434 <400> 670 atggcttctg gggcaatggg ggctgctcca aactacatgc ccacatgggc tttcgatcac cagctctcct tggacaaata cacaggtact gggcatttca gcatggacat caagttggtt ttctacctct catctcaaaa ctcagagcac agaaagcctg atcaagtact ggctttcaat gctggagatt gacgaaatag tggcggtggc tcaatggtgg ccaaggggtc ctgcagggac actttgagtt attcggtaga ctccgagata ttacctgttc agtcactgca cttg 120 180 240 294 <210> <211> <212> <213> 671 396
DNA
Eucalyptus grandis <400> 671 agagcaatca tggcccatga agcttgctgc tgatggctgc ctgacgtggg gtggcagcga tccctcgaca gagtgtcggg atcgacatgc agctcaagct ttgtcttcgc aagggccaac ggcgaccctt acatcctcca aggtggaggt cgcttngccc ccgggccaag gtcgggcttc cgtcgccggg tcacgacgag caccaacgtt cctagtgctt gcagctggga atcttcagcg cggtccaaga aactccgccg atcgacttcg ttcact cctccatggt acttctacca ggggtcaant aggagtacct gcaccgtgac agttcctggg ggtgctcgtg ggacttcgac cctgtcgctg gttcggccgg cgcttactac gaacctgagc 120 180 240 300 360 396 <210> 672 <211> 287 <212> DNA <213> Eucalyptus grandis <400> 672 gggtctggnc gcgggtgcga ggtttgagga ttttgtctac gaaggagagg ttctcaagct cgggncgaag agcaagtaca tgttcggcaa cgactcggct gggaccgtca ctgccttcta attcgacttc gagttcctgg gcaacacgac gctntaccag caagcttgac agttaccatc catgtcgtcg aggggagccc ccgggctggg ctatggacca aactactctg gcgctgggtt cagatcaagc tcgncgaggg gatggaccga accacaacga tacctgg 120 180 240 287 <210> 673 <211> 445 <212> DNA <213> Eucalyptus grandis <400> 673 gcgacttcag agctcgtgaa tcttcaatgg ctgtcccggt Cttgcgttgt tggtgggtct tgqgctatga accattttgt tctggcgctg ggttcgggtc aagctcgtcg agggcgacc ccgaaccaca acgaattcga gtacagacca acgtgtaccg gaaaagcttt cttttccaaa ggtcgcgqgt ctacnaagma gaagagcaag qgctgggacc cttcgagttc tgaac tgctctcgct gtgtctgtgt qcqaocitt ta ngagg)ttctc3l tacatgttcg gtcactgctt ctgggcaaca cttcttcttc cgttcggcct aaaacf-iagctagc gcaaagttac tctacatgtc cgacagggga ttcttcttct gttcgtgggt tgacaac taO catccagatc gtcggatgga gccctacctg <210> 674 WO 00/22092 WO 0022092PCTINZ99/O00169 <211> 387 <212> DNA <213> Eucalyptus grandis <400> 674 cacacccaat tggccaattc tttcgaataa gatggccatg Cctccggcaa cttcaacaag ccagcagtgg ccagctcctc ccaagaagca gtacttgttc ccgccggcac cgtcaccgcc acttcgagtt tctcggtaac ctctccttaa gcactggttg gactt cgaca acgctgtccc gggaagattg tattaccttt ctgagcg tttcaaaaag cccttggcct tcacgtgggg tggacaagac acatgcagct Cttctttggg ccatggcttt cttggtggcg tgatggccgt gtcggggtcg caaactcgtg ttctgcgcac caaggcgtcc gccgcggcgg gcgcagatac ggcttccggt cctgggaact gacgaaatcg 120 180 240 300 360 387 <210> 675 <211> 324 <212> DNA <213> Eucalyptus grandis <400> 675 ctcgcactcg gctggcatga gtoggtgggcc tggacccact gggctcgtcc caccagtacc tgccgccgcc atggtcgccg gatgaagggg gcgtgggtcc gcgttccata tcgagctgta ccgggaaccg tgagctggtg gcctctccct ggcactcgca agtcggtctt gacg cgccgtgtcc gttctggtgg gccggcgctg cgcgtcgatc gatgcggccg ttgcagtagt cgtagaccat aggctcagct cggacaccgt ctgcaccgct tggcgttgtc tcgaagttac ggtaggacgt cctgggtggc ccagtcgtcg <210> 676 <211> 330 <212> DNA <213> Eucalyptus grandis <400> 676 cgcctccggc cataggcatc ggccgtccga tctcacagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt gagctcattg ggacgagccc gggcgctttc ccagaggctc tccattcttg ccagatgacg attttccatg tggatccaga gtggaccagg tgcaggcagt gaccagtctg tcttgatcag gagtatattt tcatgggctg aaaactctaa taggaatgtt gtaggaagcg ggcctggaac tccgcctctc gttcttgggg gtctaccaag 120 180 240 300 330 <210> 677 <211> 438 <212> DNA <213> Eucalyptus grandis <400> 677 gtgagaaaca gagcgacttc t~ttIII~tt c WtLtLLt ttcggcctgt tcgtgggtct gagctctacc agccgggctg ctcaagcttg acaactactc aaagttacca tcgta tacatgtcgt cggatggacc acaggggagc cctacctg agagctcgtg ttCaatqgct tgcgttgttg ggctatggac tacacctaaa c ctcgag gaaccacaac aagaaaagct gccccggtct gtgggtctgg cattttgtat ttcocct ro 99cgactcgg gaattcgact tttgctctcg tttccaaagt tcgcgggtgc acgaaggaga ctgggaccgt tcgagttcct ctcttcttct gtctgtgtcg gaggtttgag ggttctcaag cactgctttc gggcaacacg <210> 678 <211> 362 <212> DNA WO 00/22092 PCT/NZ99/00169 <213> Eucalyptus grandis <400> 678 gtctggtcgc gggtgcgagg ttgtctacga aggagaggtt ggtcgaagag caagtacatg actcggctgg gaccgtcact tcgacttcga gttcctgggc acgtgaacgg ggtgggcaac ac tttgaggagc ctcaagctca ttcggcaaag gctttctaca aacacgacag cgggagcaga tctaccagcc agct tgacaa ttaccatcca tgtcgtcgga gggagcccta ggctcggcct gggctgggct ctactctggc gatcaagctc tggaccgaac cctggtacag ctggttcgac atggaccatt gctgggttcg gtcgagggcg cacaacgaat accaacgtgt cccaccactg 120 180 240 300 360 362 <210> 679 <211> 424 <212> DNA <213> Eucalyptus grandis <400> 679 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt cggagtagga gtggaagtca tgcccacccc gttcacgtac ccaggaactc gaagtcgaat tgac gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca gtggtggggt acgttggtct tcgttgtggt aagttacggt tgggtggccc gggt acggga aaaacacgac cgaaccagag gtaccaggta tcggtccatc aggacgtgat agtcgtcggc tgccccggtg ttggcgctgg gccgagcctc gggctcccct cgacgacatg gaagggggcg gttccatatc ctccaagttg ttccagagga tgctcccggt gtcgtgttgc tagaaagcag 120 180 240 300 360 420 424 <210> 680 <211> 414 <212> DNA <213> Eucalyptus grandis <400> 680 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta cccaggaact cgaagtcgaa gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgt tggtc ttcgttgtgg aagttacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccaggt ttcggtccat aggacgtgat agt cgtcggc tgccccggtg cttggcgctg ggccgagcct agggctcccc ccgacgacat gaagggggcg gttccatatc ctccaagttg gttccagagg ctgctcccgg tgtcgtgttg gtag 120 180 240 300 360 414 <210> 681 <211> 239 <212> DNA <213> Eucalyptus grandis <400> 681 ttcctgtagt atgccgtgaa gggcgctttc gtggcccaat catcggcatt ccagaggctc aagggcacgc caattgactc tqcattcttq 9tggaCCaqg tycaggcagc ggcctggaac gaccagtccg tcttgatcag tccgcctctc gagtatatct tcatgggctg gctcttgggg aaaactctga tagga~ftlt qtrtrrAA <210> 682 <211> 319 <212> DNA <213> Eucalyptus grandis <400> 682 WO 00/22092 PCTINZ99/0O 169 cccagtccca acataactta tgcttagctt cctcctgcac ggtgtatttc ctccttctgg cacccatctt ttatcaaaga agcaacaaca atatagcatc cttagtctct catgaccacc tccggggaca ttgcggggta gagcgcaccc accggagcct tcccaccac cacggaaata gatgaacgaa tagtggtaaa caatatctat cttcttaggc tcatccttca aaattcagaa tttgatcagg cgtccctgtc cctcttccga tcggcgcagt agttgtagat ccggtactgg aaggcatcga ggtcctggaa <210> 683 <211> 424 <212> DNA <213> Eucalyptus grandis <400> 683 ggccgtccga tctcgcagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt aaccacaggt agaactgctg aggatgtaag ggtcgccgct ggc c cgacgagccc gggcgctttc ccagaggctc tgcattcttg ccagatgacg ctccctgttg caggttcccc gtggaccagg gaccagtccg gagtatatct aaaactctga gagtatgtgt cccttccctt aggaactcga tgcaggcagt tcttgatcag tcatgggctg taggaatgtt ggaaatttct gagtgaagac agtcaatctc ggcctggaac tccgcctctc gctcttgggg gtctaccaag ggtggggtca gttggtgtgg gtcgtgagtt 120 180 240 300 360 420 424 <210> 684 <211;' 309 <212> DNA <213> Eucalyptus grandis <400> 684 gtctggtcgc gggtgcgagg tttgaggagc ttgtctacga aggagaggtt ctcaagctca ggtcgaagag caagtacatg ttcggcaaag actcggctgg gaccgtcact gctttctaca tcgacttcga gttcctgggc aacacgacag acgtgaacg tctaccagcc gggctgggct agcttgacaa ctactctggc ttaccatcca gatcaagctc tgtcgtcgga tggaccgaac gggagcccta cctggtacag atggaccatt gctgggttcg gtcgagggcg cacaacgaat accaacgtgt 120 180 240 300 309 <210> 685 <211> 238 <212> DNA <213> Eucalyptus grandis <400> 685 gtcgccgggc actcgcacgc gtcgatctcg aagttacggt aggacgtgat gaagggggcg tgggtccagt cggtcttgat gcggccgccc tgggtggccc agtcgtcggc gttccatatc gagctgtaga cgcccatggg ctggtccttc gggtacggga tgccccggtg ctccaagttg gtgtgcacgc ggatcggtgt ctcgtccaca agaaacacga cttggcgctg gttccaga <210> 66 <211> 515 <212> DNA 120 180 238 <400> 686 gagaaacaga gcgacttcag agctcgtgaa ttcttcttct tcttcttctt caatggctgt cggcctgttc gtgggtcttg cgttgttggt gctctaccag ccgggctggg ctatggacca caagcttgac aactactctg gcgctgggtt gaaaagcttt cccggtcttt gggtctggtc ttttgtctac cgggtcgaag tgctctcgct tccaaagtgt gcgggtgcga gaaggagagg agcaagtaca cttcttcttc ctgtgtcgtt ggtttgagga ttctcaagct tgttcggcaa 120 180 240 300 WO 00/22092 PCT/NZ99/00169 agttaccatc cagatcaagc tcgtcgaggg catgtcgtcg gatggaccga accacaacga aggggagccc tacctggtac agaccaacgt gaggctcggc ctctggttcg accccaccac cgactcggct gggaccgtca ctgctttcta attcgacttc nagttcctgg gcaacacgac gracgtgaac ggggtgggca accgggagca tgact 360 420 480 515 <210> 687 <211> 445 <212> DNA <213> Eucalyptus grandis <400> 687 ggccgtccga tctcgcagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt aaccacaggt agaactgctg aggatgtaag ggtcgccgct ggcccttgcg aagacaagta cgacgagccc gggcgctttc ccagaggctc tgcattcttg ccagatgacg ctccctgttg caggttcccc gtaag gtggaccagg gaccagtccg gagtatatct aaaactctga gagtatgtgt cccttccctt aggaactcga tgcaggcagt tcttgatcag tcatgggctg taggaatgtt ggaaatttct gagtgaagac agtcaatctc ggcctggaac tccgcctctc gctcttgggg gtctaccaag ggtggggtca gttggtgtgg gt cgtgagt t 120 180 240 300 360 420 445 <210> 688 <211> 422 <212> DNA <213> Eucalyptus grandis <400> 688 gtctggtcgc gggtgcgagg ttgtctacga aggagaggtt ggtcgaagag caagtacatg actcggctgg gaccgtcact tcgacttcga gttcctgggc acgtgaacgg ggtgggcaac acttccactc ctactccgtc ca tttgaggagc ctcaagctca ttcggcaaag gctttctaca aacacgacag cgggagcaga ctctggaacc tctaccagcc agcttgacaa ttaccatcca tgtcgtcgga gggagcccta ggctcggcct agcgccaagt gggctgggct ctactctggc gatcaagctc tggaccgaac cctggtacag ctggttcgac cgtgt ttct t atggaccatt gctgggttcg gtcgagggcg cacaacgaat accaacgtgt cccaccactg gtggacgaga 120 180 240 300 360 420 422 <210> 689 <211> 279 <212> DNA <213> Eucalyptus grandis <400> 689 tgcaaggttt gaggagctct accagccggg agaggttctc aagctcaagc ttgacaacta gtacatgttc ggcaaagtta ccatncagat cgtcactgct ttctacatgt cgtcggatgg cctgggcaac acgacagggg agccctacct ctgggctatg gaccattttg tctacgaagg ctctggcgct gggttcgggt caaagagcaa caagctcgtc tagggcgact cggctgggac accgaaccac aacgaattcg acttcgagtt ggtacagac 120 180 240 279 <210> 690 <211> 452 <2125 r)ThA <213> Eucalyptus grandis <400> 690 agcgacttca gagctcgtga agaaaagctt ttgctctcgc tcttcttctt cttcttcttc ttcttcttct tcaatggctg tcccggtctt ttccaaagtg tctgtgtcgt tcggcctgtt cgtgggtctt gcgttgttgg tgggtctggt cgcgggtgcg aggtttgagg agctctacca gccgggctgg gctatggacc attttgtcta cgaaggagag gttctcaagc tcaagcttga WO 00/22092 WO 0022092PCTINZ99/00I 69 caactactct ggcgctgggt tcgggtcgaa ccagatcaag ctcgtcgagg gcgactcggc ggatggaccg aaccacaacg aartcgactt ctacctggta cagaccaacg tgtacgtgaa gagcaagtac atgttcggca aagttaccat tgggaccgtc actgctttct acatgtcgtc cgagttcctg ggcaacacga caggggagcc <210> <211> <2 12> <213> 691 346
DNA
Eucalyptus grandis <400> 691 cgcctccggc cataggcatc ggccgtccga tctcacagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt gagctcattg ggacgagccc gggcgctttc ccagaggctc tccattcttg ccagatgacg attttccatq gtggaccagg gaccagtctg gagtatattt aaaactctaa gagtatgtgt tggatccaga tgcaggcagt tcttgatcag tcatgggctg taggaatgtt ggaaat gtaggaagcg ggcctggaac tccgcctctc gttcttgggg gtctaccaag 120 180 240 300 346 <210> 692 <211> 470 <212> DNA <213> Eucalyptus grandis <400> 692 ggccgtccga tctcgcagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt aaccacaggt agaactgctg aggatgtaag ggtcgccgct ggcccttgcg aagacaagta cgacgagccc gggcgctttc ccagaggctc tgcattcttg ccagatgacg ctccctgttg caggttcccc gtaagcggtc gtggaccagg gaccagtccg gagtatatct aaaactctga gagtatgtgt cccttccctt aggaactcga acggtgccgg tgcaggcagt tcttgatcag tcatgggctg taggaatgtt ggaaatttct gagtgaagac agtcaatctc cggagttccc ggcctggaac tccgcctctc gctcttgggg gtctaccaag ggtggggtca gttggtgtgg gtcgtgagtt 120 180 240 300 360 420 470 <210> 693 <211> 374 <212> DNA <213> Eucalyptus grandis <400> 693 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta cccaagaact cgaa gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgttggtc aagttacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccaggt aggacgtgat agtcgtcggc t gc cccggtg cttggcgctg ggccgagcct agggctcccc gaagggggcg gttccatatc ctccaagttg gttccagagg ctgctcccgg tgtcgtgttg <210> 694 <211> 409 <22 MAT <213> Eucalyptus graxidis <400> 694 gagaaacaga gcgacttcag agctcgtgaa gaaaagcttt tgctctcgct cttcttcttc ttcttcttct tcttcttctt caatggctgt cccggtcttt tccaaagtgt ctgtgtcgtt cggcctgttc gtgggtcttg cgttgttggt gggtctggtc gcgggtgcga ggtttgagga gctctaccag ccgggctggg ctatggacca ttttgtctac gaaggagagg ttctcaagct WO 00/22092 PTN9/06 PCT/NZ99/00169 caagcttgac aactactctg gcgctgggtt agttaccatc cagatcaagc tcgtcgaggg catgtlcgt cg gatgg accga accacaacga cgggtcgaag agcaagtaca tgttcggcaa cgactcggct gggaccgtca ctgctttcta atccgacttc gagttcctg 300 360 409 <210> <211> <212> <213> 695 224
DNA
Eucalyptus grandis <400> 695 tgccccggtg ctccaagttg gtgtgcacgc cttggcgctg gttccagagg acggagtagg ggccgagcct ctgctcccgg ttgcccaccc agggctcccc tgtcgtgttg cccaggaact ggatcggtgt ctcgtccaca agaaacacga agtggaagtc agtggtgggg tcgaaccaga cgttcacgta cacgttggtc tgtaccaggt cgaagtcgaa ttcg <210> 696 <211> 442 <212> DNA <213> Eucalyptus grandis <400> 696 gtctggtcgc gggtgcgagg ttgtctacga aggagaggtt ggtcgaagag caagtacatg actcggctgg gaccgtcact tcgacttcga gttcctgggc acgtgaacgg ggtgggcaac acttccactc ctactccgtc caccgatccc gcgtgcacac tttgaggagc ctcaagctca ttcggcaaag gctttctaca aacacgacag cgggagcaga ctctggaacc ca tctaccagcc agcttgacaa ttaccatcca tgtcgtcgga gggagcccta ggctcggcct agcgccaagt gggctgggct ctact ctggc gatcaagctc tggaccgaac cctggtacag ctggttcgac cgtgtttctt atggaccatt gctgggttcg gtcgagggcg cacaacgaat accaacgtgt cccaccactg gtggacgaga 120 180 240 300 360 420 442 <210> 697 <211> 408 <212> DNA <213> Eucalyptus grandis <400> 697 gagaaacaga cgacttcaga tcttcttctt cttcttcttc ggcctgttcn tgggtcttgc ctctaccagc cgggctgggc aagcttgaca actactctgg gttaccatcc agatcaagct atgtcgtcgg atggaccgaa gctcgtgaag aatggctgtc gttgntggtg tatggaccat cgctgggttc cgtcgagggc ccacaacgaa aaaagctttt ccggtctttt ggtctggtcg tttgtctacg gggtcgaaga gactcggctg ttcgacttcq gctctcgctc ccaaagtgtn cgggtgcgag aaggagaggt gcaagtacat ggaccgtcac agttcctg ttcttcttct tgtgtcgttc gtttgaggag tctcaagctc gttcggcaaa tgctttctac 120 180 240 300 360 408 <210> 698 <211> 469 <212. MJJ1k <213> Eucalyptus grandis <400> 698 ttctItcttct tcaatggctg cgtgggtctt gcgttgttgg gccgggctgg gctatggacc caactactct ggcgctgggt ccagatcaag ctcgtcgagg ggatggaccg aaccacaacg agaaaL.ct t cccggt c tt tgggtctagt attttgtcta tcgggtcgaa gcgactcggc aattcgactt ttqCtCLCyc ttccaaagtg cgcgggtgcg cgaaggagag gagcaagtac tgggaccgtc cgagttcctg Ccccttctt tctgtgtcgt aggt ttgagg gttctcaagc atgttcggca actgctttct ggcaacacga cttcttcttC tcggcctgtt agctctacca tcaagcttga aagttaccat acatgtcgtc caggggagcc 120 180 240 300 360 420 WO 00/22092PC/Z/016 PCT/NZ99/00169 ctacctggta cagaccaacg tgtacgtgaa cg-gggtgggc aaccgggag <210> 699 <211> 347 <212> DNA <213> Eucalyptus grandis 469 <400> 699 ccgcgcagta gtcgtagatc aggcatcgag ctcattgatt cacaggagga cgagcccgtg ccgtgaaggg cgctttcgac cggcattcca gaggctcgag ttgactctcc attcttgaaa atgaagtact ttccatgtgg gaccaggtgc cagtctgtct tatattttca actctaatag tctggaccca atccagagta aggcagtggc tgatcagtcc tgggctggtt gaatgttgtc cctgagccgc ggaagcgggc ctggaacttc gcctctcgtg ct tggggaag taccaag ctccggccat cgtccgatct ctgtagtatg gcccaatcat ggcacgccaa <210> 700 <211> 452 <212> DNA <213> Eucalyptus grandis <400> 700 agcgacttca gagctcgtga ttcttcttct tcaatggctg cgtgggtctt gcgttgttgg gccgggctgg gctatggacc caactactct ggcgctgggt ccagatcaag ctcgtcgagg ggatggaccg aaccacaacg ctacctggta cagaccaacg agaaaagctt tcccggtctt tgggtctggt attttgtcta tcgggtcgaa gcgactcggc aattcgactt tgtacgtgaa ttgctctcgc ttccaaagtg cgcgggtgcg cgaaggagag gagcaagtac tgggaccgt c cgagttcctg cg tcttcttctt tctgtgtcgt aggtttgagg gttctcaagc atgttcggca actgctttct ggcaacacga CttcttcttC tcggcctgtt agctctacca tcaagcttga aagttaccat acatgtcgtc caggggagcc 120 180 240 300 360 420 452 <210> <211> <212> <213> 701 323
DNA
Eucalyptus grandis <400> 701 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt cggagtagga gtggaagtca tgcccacccc gttcacgtac gtcgatctcg gcggccgccc ctggtccttC ctcgtccaca gtggtggggt acg aagttacggt tgggtggccc gggtacggga agaaacacga cgaaccagag aggacgtgat agtcgt cggc tgccccggtg cttggcgctg gccgagcctc gaagggggcg gttccatatc ctccaagttg gtccagagga tgctcccggt 120 180 240 300 323 <210> 702 <211> 441 <212> DNA <213> Eucalyptus grandis <400> 702 cacctccaac cataaor-tr n~rt-r-=t-rT ggccgtccga tctcacagga ggacgagccc ttcctgtagt atgccgtgaa gggcgctttc gtggcccaat catcggcatt ccagaggctc aagggcacgc caattgactc tccattcttg aagatgatgt gctggggctt ccagatgacg aaccacagta gaactgctgc tccctgttgC ggatgtaagg gtcgccgctc a gtggaccagg gaccagtctg gagtatattt aaaactctaa gagtatgtgt ccttcccttg Lycaggcayt tcttgatcag tcatg-ggctg taggaatgtt ggaaatttct agtgaagacg ggcctggaac tccgcctctc gttcttgggg gtctaccaag ggtggggtca ttggtgtgga C m 120 180 240 300 360 420 441 WO 00/22092PCNZ9016 PCT/NZ99/00169 <210> 703 <211> 34S <z212> DNA <213> Eucalyptus grandis <400> 703 aggacgtgat gaagggggcg agtcgtcggc gttccatatc tgccccggtg ctccaagttg cttggcgctg gttccagagg ggccgagcct ctgctcccgg agggctcccc tgtcgtgttg tgggtccagt gagctgtaga gtgtgcacgc acggagtagg ttgcccaccc cccaggaact cggtcttgat cgcccatggg ggatcggtgt agtggaagtc cgttcacgta cgaagtcgaa gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgttggtc ttcgt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgt a ccagg t 120 180 240 300 345 <210> 704 <211> 339 <212> DNA <213> Eucalyptus grandis <400> 704 gtcgccgggc actcgcacgC tgggtccagt cggtcttgat gagctgtaga cgcccatggg tgtgcacgcg gatcggtgtc cggagtagga gtggaagtca tgcccacccc gtcacgtaca gtcgatctcg gcggccgccc C tggt cc tt c tcgtccacaa gtggtggggt cgttggtctt aagttacggt tgggtggccc gggtacggga gaaacacgac cgaaccanag taccaagta aggacgtgat agtcgtcggc tgccccggtg ttggcgctgg gccgaacctc gaagggggcg gttccatatc ctcaagttgg ttccagagga tgcttccggt 120 180 240 300 339 <210> 705 <211> 471 <212> DNA <213> Eucalyptus grandis <400> 705 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta cccaggaact cgaagtcgaa gtgacggtcc cagccgagtc gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgttggtc ttcgttgtgg gccctcgacg aagttacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccaggt ttcggtccat agcttgatct aggacgtgat agtcgtcggc tgccccggtg cttggcgctg ggccgagcct agggctcccc ccgacgacat ggatggtaac gaagggggcg gttccatatc ctccaagttg gttccagagg ctgctcccgg tgtcgtgttg gtagaaagca 120 180 240 300 360 420 471 <210> 706 <211> 484 <212> DNA <213> Eucalyptus grandis <400> 706 ccgcgcagta gtcgtagatc Lcaa9aqqa cgagcccgtg ccgtgaaggg cgctttcgac cggcattcca gaggctcgag ttgactctcc attcttgaaa ggggcttcca gatgacggag actgctgctc cctgttgccc cgcc atgaagtact gaccaggtgc cagtctgtct tatattttca actctaatag tatgtgtgga ttcccttgag tctggaccca aggcagtggc tgatcagtcc tgggctggtt gaatgttgtc aatttctg tgaagacgtt cctgagccgc crggaacttc gcctctcgtg cttggggaag taccaagaag ggggtcaaac ggtgtggagg ctccggccat ctgtagtatg gcccaatcat ggcacgccaa atgatgtgct cacaggtaga atgtaagggt 180 240 300 360 420 480 484 WO 00/22092 WO 0022092PCT/NZ99/0OI 69 <210> 707 <211> 317 <212> DNA <213> Eucalyptus grandis <400> 707 cgggtacggg atgccccggt gctccaagtt aagaaacacg acttggcgct ggttccagag gtcgaaccag aggccgagcc tctgctcccg ctgtaccagg tagggctccc ctgtcgtgtt gttcggtcca tccgacgaca tgtagaaagc gagcttgatc tggatgg ggtgtgcacg gacggagtag gttgcccacc gcccaggaac agtgacggtc cggatcggtg gagtggaagt ccgttcacgt tcgaagtcga ccagccgagt tctcgtccac cagtggtggg acacgttggt attcgttgtg cgccctcnac 120 180 240 300 317 <210> <211> <212> <213> 708 367
DNA
Eucalyptus grandis <400> 708 ggccgtccga tctcgcagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt aaccacaggt agaactgctg aggatgt cgacgagccc gggcgctttc ccagaggctc tgcattcttg ccagatgacg ctccctgttg gtggaccagg gaccagtccg gagtatatct aaaactctga gagtatgtgt cccttccctt tgcaggcagt tcttgatcag tcatgggctg taggaatgtt ggaaatttct gagtgaagac ggcctggaac tccgcctctc gctcttgggg gtctaccaag ggtggggtca gttggtgtgg <210> <211> <212> <213> 709 384
DNA
Eucalyptus grandis <400> 709 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaaa cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta cccaggaact cgaagtcgaa gtcgatctcg gcggccgccc ctggtccttC ctcgtccaca agtggtgggg cacgttggtc t t c aagt tacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccaggt aggacgtgat agtcgtcggc tgccccggtg cttggcgctg ggccgagcct agggctcccc gaagggggcg gttccatatc ctccaagttg gttccagagg ctgctcccgg tgtcgtgttg <210> <211> <212> <213> 710 364
DNA
Eucalyptus grandis <400> 710 gtcgccgggc actcgcacgc gagcugtaga cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta ccca gtcgatctcq ctggtccttc ctcgtccaca agtggtgggg cacgttggtc aagttacqgt gggtacggga agaaacacga tcgaaccaga tgtaccaggt aggacgtgat &qCgz~.qqC tgccccggtg ct tggcgctg gccga 9cc t agggctcccc gaagggggcg q LLCC&t~tC ctccaagttg gttccagagg ctgctcccgg tgtcgtgttg 180 240 300 360 364 <210> 711 WO 00/22092 PCT/NZ99/00 169 <211> 338 <212> DNA <2.13> Eucalyptus grandis <400> 711 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgttggtc aagttacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccag aggacgtgat agtcgtcggc tgccccggtg cttggcgctg ggccgagcct gaagggggcg gttccatatc ctccaagttg gttccagagg ctgctcccgg 120 180 240 300 338 <210> 712 <211> 216 <212> DNA <213> Eucalyptus grandis <400> 712 ggcccaatca tcggcattcc agaggctcga gggcacgcca attnnctctc cattcttgaa gatgatgtgc tggggcttcc agatgacgga ccacaggtag aactgctgct ccctgttgcc gtatattttc atgggctggt tcttggggaa aactCtaata ggaatgttgt ctaccaagaa gtatgtgtgg aaatttctgg tggggtcaaa cttccc <210> <211> <212> <213> 713 341
DNA
Eucalyptus grandis <400> 713 gtcgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt acggagtagg agtggaagtc ttgcccaccc cgttcacgta gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgttggtc aagttacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccaggt aggacgtgat agtcgtcggc tgccccggtg cttggcgctg ggccgagcct a gaagggggcg gttccatatc ctccaagttg gttccagagg ct gc t cccgg 120 180 240 300 341 <210> 714 <211> 413 <212> DNA <213> Eucalyptus grandis <400> 714 gccgccgggc actcgcacgc tgggtccagt cggtcttgat gagctgtaga cgcccatggg gtgtgcacgc ggatcggtgt ~acggaggg aqtgg aaguc ttgcccaccc cgttcacgta cccaggaact cgaagtcgaa gtcgatctcg gcggccgccc ctggtccttc ctcgtccaca agtggtgggg cacgttggtc ttcgttgtgg aagttacggt tgggtggccc gggtacggga agaaacacga tcgaaccaga tgtaccaggt ttcggtccat aggacgtgat agtcgtcggc tgccccggtg ct tggcg ctg ggccgagcct agggctcccc ccgacgacat gaagggggcg gttccatatc ctccaagttg gttccagagq ctgctccgg tgtcgtgttg gta <210> 715 <211> 280 <212> DNA <213> Eucalyptus grandis <400> 715 tctggtcgcg ggtgcgaggt ttgaggagct ctaccagccg ggctgggcta tggaccattt WO 00/22092 WO 0022092PCT/NZ99/001 69 tgtctacnaa ggagaggttc tcaagctcaa gtcgaagagc aagtacatgt tcggcaaaga ctoggc tggg accgrncactg ctttctacat cgacttcgag ttcctgggca acacgacagg gcttgacaac tactctggcg ctgggttcgg taccatccag atcaagCtCg tcgagggcga gtcgtcggat ggaccgaacc acaacgaatt ggagccctac <210> 716 <211> 397 <212> DNA <213> Eucalyptus grandis <400> 716 ccgcgcagta gtcgtagatc aggcatcgag ctcattgatt cacaggagga cgagcccgtg ccgtgaaggg cgctttcgac cggcattcca gaggctcgag ttgactctcc attcttgaaa ggggcttcca gatgacggag atgaagtact ttccatgtgg gaccaggtgc cagtctgtct tatattttca actctaatag tatgtgtgga tctggaccca atccagagta aggcagtggc tgatcagtcc tgggctggtt gaatgttgtc aa ttt ct cctgagccgc ggaagcgggc ctggaacttc gcctctcgtg cttggggaag taccaagaag ctccggccat cgtccgatct ctgtagtatg gcccaatcat gg ca c gcca a atgatgtgct 120 180 240 300 360 397 <210> 717 <211> 365 <212> DNA <213> Eucalyptus grandis <400> 717 ggccgtccga tctcgcagga ttcctgtagt atgccgtgaa gtggcccaat catcggcatt aagggcacgc caattgactc aagatgatgt gctggggctt aaccacaggt agaactgctg aggat cgacgagccc gggcgctttc ccagaggctc tgcattcttg ccagatgacg ctccctgttg gtggaccagg gaccagtccg gagtatatct aaaactctga gagtatgtgt cccttccctt tgcaggcagt tcttgatcag tcatgggctg taggaatgtt ggaaatttct gagtgaagac ggcctggaac tccgcctctc gctcttgggg gtctaccaag ggtggggtca gttggtgtgg 120 180 240 300 360 365 <210> 718 <211> 301 <212> DNA <213> Eucalyptus grandis <400> 718 cgcctccggc cataggcatc gagctcattg ggccgtccga tctcacagga ggacgagccc ttcctgtagt atgccgtgaa gggcgctttc gtggcccaat catcggcatt ccagaggctc aagggcacgc caattgactc tccattcttg a attttccatq gtggaccagg gaccagtctg gagtatattt aaaactctaa tggatccaga tgcaggcagt tcttgatcag tcatgggctg taggaatgt t gtaggaagcg ggcctggaac tccgcctctc gttcttgggg gtctaccaag <210> 719 <211> 383 <212> DNA <213> P-iir 1%rntiiq r-nrlic <400> 719 cgcctccggc cataggcatc gagctcattg ggccgtccga tctcacagga ggacgagccc ttcctgtagt atgccgtgaa gggcgctttc gtggcccaat catcggcatt ccagaggctc aagggcacgc caattgactc tccattcttg attttccatg gtggaccagg gaccagtctg gagiatatit aaaactctaa tggatccaga tgcaggcagt tcttgatcag tcatgggctg taggaatgtt gtaggaagcg ggcctggaac tccgcctctc gttcttgggg gtctaccaag WO 00/22092 WO 0022092PCTINZ99/00 169 aagatgatgt gctggggctt ccagatgacg gagtatgtgt ggaaatttct ggtggggtca aaccacaggt agaactgctg ctc 360 383 <210> <211> <212> <213> 720 370
DNA
Eucalyptus grandis <400> 720 cgacagagtg tcggggtcgg catgcagctc aagctcgtcg ttcgcaaggg ccaactcacg cccttacatc ctccacacca ctacctgtgg tttgacccca gcacatcatc ttcttggtag tggcgtgccc gcttccggtc ccgggaactc atgagattga acgtcttcac ccagaaattt acaacattcc caagaaggag cgccggcacc c-.tcgagttc tcaagggaag ecacacatac tattagagtt tacctgttcg gtgaccgctt ctggggaacc ggcaacaggg tccgtcatct ttcaagaatg gccggatcga actacttgtc tgagcggcga agcagcagtt ggaagcccca gagagtcaat 120 180 240 300 360 370 <210> 721 <211> 413 <212> DNA <213> Eucalyptus grandis <400> 721 ggcggccgag gaagaagggc gaagccgctg aaggaggcgg ccgggtggcc cagtcctcag tgggtagggg acccccgccg aaagtacctg ttttttttag aaatcggtgt tattttagaa gagtacttac acgacgtgta aagtggaggc tgaaaggcgc cgtcccatag catccaagtt cagaaagaga ggaaatttat aagggttcca tccgttggac ctgcgaccag ggtcgagtag cttgaactcc tccgtcattc ttgttcaaag aaggacggag cagacgcaag tcagtcttca agggtcatgg cttattggaa attatgtggc gttttcatga taagtgtgga cgctcgcgtt cgaggccgcc gctggctctt tcccatcgac gaaggacctc tcaggacgag aat 120 180 240 300 360 413 <210> 722 <211> 393 <212> DNA <213> Eucalyptus grandis <400> 722 ttacattcgg acgggtcgac ggcgaccctt atatcctcca caattctatc tctggttcga cctttacacg tcgtgtactt gcggcggggg tcccctaccc gctgaggact gggccacccg accgcctcct tcagcggctt ctgggatgag caccaacttg cccgacagct tgtcgatggg aaagagccag gggcggcctc caacgcgagc atagacttgg tacagccaag gatttccaca attccaataa cccatgaccc gtgaagactg gct agttcttggg gcaaaggcaa cttactccgt gggagttcaa tctactcgac actggtcgca gaacttgagc tagggagcag cctttggaac gaacttggat cctatgggac ggcgcctttc 120 180 240 300 360 393 <210> 723 <211> 244 <212> DNA <213> Eucalyptus grandis gcaataggga gcagcaattc tatctctggt tcgacccgac agctgatttc cacacttact cgtcctttg gaacccttta cacgtcgtgt actttgtcga tclggattcca ataagggagt tcaagaactt ggatgcggcg ggggtcccct acccaaagag ccagcccatg accctctact cgaccctatg ggacgctgag gactgggcca cccggggcgg cctcgtgaag actgactggt cgca 120 IS0 240 244 WO 00/22092 PCT/NZ99/00I 69 <210> 724 <211> 238 <212> DNA <213> Eucalyptus grandis <400> 724 gacgagacac cgatccgcgt gcacaccaac gaccagccca tgggcgtcta cagctcgata ggccgcatca agaccgactg gacccacgcc atcgacgcgt gcgagtgccc ggcgaccatg ttggagcacc ggggcatccc gtacccgaag tggaacgccg acgactgggc cacccagggc cccttcatca cgtcctaccg taacttcgag gcggcggcag acaacgccaa gcggtgca 120 180 238 <210> <211> <212> <213> 453
DNA
Eucalyptus grandis <400> 725 tggtggcggc cgcggcggcc atggccgtgc gcagataccc cggggtcggg cttccggtcc aactcgtgcc tgggaactcc ctgcgcacga cgaaatcgac tccatacgaa cgtgttcacg tcgaccccac caaggacttc tctctgtcga tggaacgccc tccggcaact agcagtggcc aagaagcagt gccggcaccg ttcgagtttc caaggaaaag cacacctact ataagagagt tcaacaagga agctcctcac acttgttcgg tcaccgctta tcggtaacct gaaacagaga ccatcctgtg tca cttcgacatc gctgtccctg gaagattgac ttacctttct gagcggcgac acaacagttc gaaccctcaa acgtggggtg gacaagacgt atgcagctca tctttgggtt ccgtacaccc catctctggt agcatcatct 120 180 240 300 360 420 453 <210> 726 <211> 334 <212> DNA <213> Eucalyptus grandis <400> 726 gccgatccgg gtgttcaaga gatgaaattg tactccagcc gaagaccgac tggtccaagg gtgcgaagcg tcggttgagg gaaggagttc caggacctcg atacaccatc tacaactact acagcaagga tgtggaatgc cgccgttcgt ccaagttctg atgccttcca gcgctgtcgg ccttggggtg ggatgactgg ggcctcttac cgctactcag gtaccggagg aaga aaattcccct gccactcggg cgggggttcc ggccagaggt ctccggtagg tcaaccagcc gagggcttga acatcgacgg ggtgggacca tgcgctcgaa 120 180 240 300 334 <210> 727 <211> 416 <212> DNA <213> Eucalyptus grandis <400> 727 accgtcaccg catactattt ttcttgggga acttgagcgg aagggtaacc gagagcagca tactccatcc tctggaatcc tttgCgatCa ttycayattc agtccatcgg tgttcctttc atcttcaaac cgatccatac attctatctc acaacgcatc tcagtggacg ccaaggccca gggtcggcgt attctccaca tggttcgacc atgtaagagt ggactcccat gcccatgagg gggatgagat ccaacgtctt caactgctga ctgaaaagct cagagagttc atattcttcg agacttcgag cagccaaggc tttccacacc caaaatgcga aagaacgcag agccct <210> 728 <211> 375 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/001 69 <400> 728 acaaaacqcg cccaatatgg tctcatggtc gtttcttggg cataacctgg ggagatggcc ccttgacaag gcctccggct tgacatgcag ctcaaacttg atcttcaaat gggtcgacgt cgatccgtac attct cctctcgttc cgact tctgc gaggtcagat ccggcttcca ttcctggcaa gggacgagat cacttcttcg ttccgcccgC cctcaacaat gtccaagaac ctctgctggC agatttcgag ctttgtagag aacttctatc ggcgacctcc gagtacttgt accgtcaccg ttcttgggga ctgcgcttct aagacttcga tcactctctc tcggcaagat catactattt atttaagtgg 120 180 240 300 360 375 <210> <211> <212> <213> 729 538
DNA
Eucalyptus grandis <400> 729 agaaggagta ccggcaccgt tcgagttcct aagggaaggg acacatactc ttagagtttt aaatatactc cagactggtc cctggtccac cctgttcggc gaccgcttac ggggaacctg caacagggag cgtcatctgg caagaatgga gagcctctgg gaaatcgccc gggctcgtcc cggat cgaca tacttgtctt agcggcgacc cagcagttct aagccccagc gagtcaattg aatgccgatg ttcacggcat tcctgtgaga tgcagctcaa cgcaagggcc cttacatcct acctgtggtt acatcatctt gcgtgccctt attgggccac actacaggaa tcggacggcc gctcgtcgcc aactcacgat ccacaccaac tgaccccacc cttggtagac ccccaagaac gagaggcgga gttccaggcc cgcttcctac gggaactccg gagattgact gtcttcactc agaaatttcc aacattccta cagcccatga ctgatcaaga actgcctgca tctggatc <210> 730 <211> 412 <212> DNA <213> Eucalyptus grandis <400> 730 ccggatcgac atgcagctca ctacttgtct tcgcaagggc gagcggcgac ccttacatcc gcagcagttc tacctgtggt gaagccccag cacatcatct agagtcaatt ggcgtgccct gaatgccgat gattgggcca agctcgttgc caactcacga tccacaccaa ttgaccccac tcttggtaga tccccaagaa cgagaggcgg cgggaactcc cgagatcgac cgtcttcact caggaatttc caacattcct ccagcccatg ctgatcaaga gccggcaccg ttcgagttcc caagggaagg cacacatact attagagttt aaaatatact cagactggtc tgaccgctta tggggaacct gcaacaggga ccgtcatctg tcaagaatgg cgagcctctg ga 120 180 240 300 360 412 <210> <211> <212> <213> 731 350
DNA
Eucalyptus grandis <400> 731 acaccgatcc gcgtgcacac cccatgggcg tctacagctc atcaagaccg actggaccca ggCagggaga qqCqgLactg aaccaccagc tcaagtgggt caacttggag gatatggaac cgcccccttc gtgggacgag ccaggcccac caccggggca gccgacgact atcacgtcct cccacggtgt cacatggtct tcccgtaccc gggccaccca accgtaactt C~aaLjuyLy ccgagctgag acgactactg gaaggaccag gggcggccgc cgagatcgac c-;YcaqCYCC cctccaccag <210> 732 <211> 354 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/00I 69 <400> 732 ctcctgtcgc tgtccctcga ctgttcggcc ggatcgacat accgcttact acttgtcttc gggaacctga gcggcgaccc aacagggagc agcagttcta gtcatctgga agccccagca cagagtgtcg gcagctcaag gcaagggcca ttacatcctc cctgtggttt catcatcttc gggtcgggct Ctcgtcgccg actcacgatg cacaccaacg gaccccacca ttggtagcaa tccggtccaa ggaactccgc agattgactt tcttcactca gaaatttcca cattcctatt gaaggagtac cggcaccgtg cgagttcctg agggaagggc cacatactcc agag <210> <211> <212> <213> 733 480
DNA
Eucalyptus grandis <400> 733 cccacatctc tccctcctct actacagcag cagcagaaag ctcactttgc atcactacac ccgcaacttc tatcaagact caacqgcgac ctcctcactc gaacgagtac ttgttcggca aggcactgtc accgcatact cgagttcttg gggaacttga tcagtcctca acagacagaa tgcttctcgt tcgacataac tctcccttga agattgacat atttatcttc gcggcgatcc ctctcatatc cacgcccaat ggtcgtttct ctggggtgat caaggcctcc gcagctcaag aaatgggtcg atacattctt atctttgctt atggccttcc tgggcgacgt ggccgagctc ggctctggct ctcgttcccg acgtgggacg cacaccaacg ctcttcagca cttctacttc ctgcttccgc agatcctcaa tccagtccaa gcaactccgc agatagactt tgttcagcca 120 180 240 300 360 420 480 <210> 734 <211> 343 <212> DNA <213> Eucalyptus grandis <400> 734 ccgcgtcaag gtactggata ctccggcttc aagtccaaga cgtccccggc aactccgcgg ctgggatgag atagacttgg caccaacttg tacagccaag cccgacagct gatttccaca acggccagct accagtacct gcactgtcac agttcttggg gcaaaggcaa cttactccgt cgtcaccctt ctatggcaaa caccttatac gaacttgagc tagggagcag cctttggaac tccctggaca ttcgacatgc ttacattcgg ggcgaccctt caattctatc cct aggcctctgg aaatcaagct acgggt cgac atatcctcca tctggttcga <210> 735 <211> 359 <212> DNA <213> Eucalyptus grandis <400> 735 atccaagggg tcttacctgt ttctgcaggg acagtcactg agactttgag ttcttgggta cacgggagga aaaggagaca ctaccactcc tactctgttc gccgatccgg gtgttcaaga tcgggcattt cattctacct acaggagtgg gagaacagag tctggaacat acagcaagga cagcatggac ctcatctcaa gcagccgtac gatttatctt gtaccagatt ccttggggtg atcaagttgg aactcagagc atagtgcaga tggttcgatc gtattcttcg aaattcccct ttgctggaga acgacgaaat ccaatgtgtt ccaccgccgc tggatgacgt tcaaccagc ,-2:10 <211> <212> <213> 736 360
DNA
Eucalyptus grandis <400> 736 gcatactatt tatcttcaaa tgggtcaacg tgggacgaga tagacttcga gttcttgggg WO 00/22092 WO 0022092PCT/NZ99/00169 aacttgagcg gcgatcccta cattcttcac cgagagcagc aattctatct ctggttcgac ctctggaatc cacaacgcat catattctca aacgcagagt ccatcggtgt tcctttcccc ctctggaacg cagacgactg ggcgaccaga accaacgtgt tcagccaagg caagggcaac ccaactgctg atttccacac ctactccatc gtggacggga ctccgatcag agagttcaag aaggcccagc ccatgaggat attctcgagc ggcggcctag tgaagacgga cttggacgca 120 180 240 300 360 <210> 737 <211> 437 <212> DNA <213> Eucalyptus grandis <400> 737 gcgacttcag agctcgtgaa atggctgtcc tggtcttttc ttgttggtgg gtctggtcgc atggaccatt ttgtctacga gctgggttcg ggtcgaagag gtcgagggcg actcggctgg cacaacgaat tcgacttcga accacgtgtc cgtgacg gaaaagcttt caaagtgtct gggtgcgagg aggagaggtt caagtacatg gaccgtcact gttcctgggc tgctctcgct gtgtcgttcg tttgaggagc ctcaagctca ttcggcaaag gctttctaca aacacgacag Cttcttcttc gcctgttcgt tctaccagcc agcttgacaa ttaccatcca tgtcgtcgga gggagcccta ttcttcttca gggtcttgcg gggctgggct ctactctggc gatcaagctc tggaccgaac cctggtacag 120 180 240 300 360 420 437 <210> 738 <211> 341 <212> DNA <213> Eucalyptus grandis <400> 738 ggcgaccctt acatcctcca cagttctacc tgtggtttga ccccagcaca tcatcttctt tcaattggcg tgcccttccc gccgatgatt gggccacgag acggcatact acaggaagtt caccaacgtc ccc ca cc agg ggtagacaac caagaaccag aggcggactq ccaggccact ttcactcaag aatttccaca attcctatta cccatgaaaa atcaagacag gcctgcacct ggaagggcaa cagggagcag catactccgt catctggaag gagttttcaa gaatggagag tatactcgag cctctggaat actggtcgaa agcgcccttc g 120 180 240 300 341 <210> <211> <212> <213> 739 497
DNA
Eucalyptus grandis <400> 739 gagcttgctg cctgacgtgg gtccctcgac gatcgacatg cttgtcttcg cggcgaccct gcagttctac gccccagcac gtcaattggc ctgatggctg ggtggcagcg agagtgtcgg cagctcaagc caagggccaa tacatcctcc ctgtggtttg atcatcttct gtgccct ccgnttcgcc accgcgccaa ggtcgggctt tcgttgccgg ctcacgacga acaccaacgt accccaccag tggtagacaa cgcagctggg gatcttcagc ccggtccaag gaactccgcc gatcgacttc cttcactcaa gaatttccac cattcctatt aacttctacc gggggtcagc aaggagtacc ggcaccgtga gagttcctgg gggaagggca acatactccg agagttttca aggacttcga tcctgtcgct tgttcggccg ccgcttacta ggaacctgag acagggagca tcatctggaa agaatggaga 120 180 240 300 360 420 480 497 <21±0> 740 <211> 497 <212> DNA <213> Eucalyptus grandis <400> 740 ccaagaagga gtacctgttc qgccggatcg acatgcagct caagctcgtc gccgggaact WO 00/22092 WO 0022092PCT/NZ99/00 169 ccgccggcac acttcgagtt ctcaagggaa tccacacata ctattagagt tgaaaatata agacagactg gccacgggct cgtgaccgct cctggggaac gggcaacagy ctccgtcatc tttcaagaat ctcgagcctc gtcgaaatcg gtctctg tactacttgt ctgagcggcg gagcagcagt tggaagcccc ggagagtcaa tggaatgccg ccttacggat cttcgcaagg acccttacat tctacctgtq agcacatcat ttggcgtgcc atgattgggc actacagaag qccaactcac cctccacacc gtrtgacccc cttcttggta cttccccaag cacgagaggc ttcaggccac gatgagattg aacgtcttca accagaaatt gacaacattc aaccagccca ggactgatca tgctgacctg 120 180 240 300 360 420 480 497 <210> 741 <211> 395 <212> DNA <213> Eucalyptus grandis <400> 741 cgcaactttt atcaagactt caatggcgac ctcctcactc gaacgagtac ttgttcggca aggcactgtc accgcatact cgagttcttg gggaacttga aggcaagggc aaccgagagc cacctactcc atcctctgga tttcataacc tctcccttga agattgacat atttatcttc gcggcgatcc agcaattcta atccacaacg tgggggagat caaggcctcc gcagctcaag aaatgggtca ctacattctt tctctggttc catta ggccgagctc ggctccggct ctcgttcctg acgtgggacg cacaccaacg gacccaactg agatcctcaa tccagtccaa gcaactccgc agattgactt tgttcagcca ctgatttcca <210> 742 <211> 396 <212> DNA <213> Eucalyptus grandis <400> 742 ggcggccgag gaagaagggc gaagccgctg aaggaggcgg ccgggtggcc cagtcctcag tgggtagggg acccccgccg aaagtacctg ttttttttag aaatcggtgt tattttagaa agtacttaca cgacgtgtaa aagtggaggc tgaaaggcgc cgtcccatag catccaagtt cagaaagaga ggaaatttat agggt tcc aa tccgttggac ctgcgaccag ggtcgagtag cttgaactcc tccgtcattc ttgtcaaagg aggacg cagacgcaag tcagtcttca agggtcatgg cttattggaa attatgtggc ttttcatgat cgctcgcgtt cgaggccgcc gctggctctt tcccatcgac gaaggacctc caggacgagg <210> 743 <211> 347 <212> DNA <213> Eucalyptus grandis <400> 743 caccacctta tacttacatt ggggaacttg agcggcgacc caatagggag cagcaattct cqLcctttqg aaccctttac caagaacttg gatgcggcgg gaccctatgg gacgctgagg cggacgggtc cttatatcct atctctggtt acgtcgtgt a gggtccccta actgggccac gacctgggat ccacaccaac cgacccgaca ctttgtcgat cccaaagagc CCggggcggc gagatagact t tgt a cagc c gctgatttcc gggattccaa cagcccatga ctcgtga tggagttctt aaggcaaagg acacttactc taagggagtt ccctctactc 120 180 240 300 347 <210> 7144 <211> 446 <212> DNA <213> Eucalyptus grandis <400> 744 gtcaaggtac tggataacgg ccagctcgtc accctttccc tggacaaggc ctctggctcc WO 00/22092 WO 0022092PCT/NZ99/001 69 ggcttcaagt cccggcaact gatgagatag aacttgtaca acagctgatt gatgggattc agccagccca ccaagaacca ccgcgggcac acttggagtt gccaaggcaa tccacactta caataaggga tgaccctcta gtacctctat tgtcaccacc ct tggggaac aggcaatagg ctccgtcctt gttcaagaac ctcgac ggcaaattcg ttatacttac t tgagcggcg gagcagcaat tggaaccctt ttggatgcgg acatgcaaat attcggacgg acccttatat tctatctctq tacacgtcgt Cgggggtccc caagctcgt c gtcgacctgg cctccacacc gttcgacccg gtactttgtc ctacccaaag 120 180 240 300 360 420 446 <210> 745 <211> 439 <212> DNA <213> Eucalyptus grandis <400> 745 ccagtctctc tcccccactc atagagcaat catggcccat tgagcttgct gctgatggct acctgacgtg gggtggcagc tgtccctcga cagagtgtcg ggatcgacat gcagctcaag acttgtcttc gcaagggcca gcggngaccc ttacatcct tttctcaagc gaaggtggag gccgcttcgc gaccgggcca gggtcgggct ctcgtcgccg actcacgatg caaaatataa gtcctagtgc ccgcagctgg agatcttcag tccggtccaa ggaactccgc agattgactt gccaaagtat ttcctccatg gaacttctac cgggggtcag gaaggagtac cggcaccgtg cgagttcctg aagccaaagt gtggtgctcg caggacttcg ctcctgtcgc ctgttcggcc accgcttact gggaacctga 120 180 240 300 360 420 439 <210> 746 <211> 322 <212> DNA <213> Eucalyptus grandis <400> 746 acgacgagat cgacttcgag ccaacgtctt cactcaaggg ccaccaggaa tttccacaca tagacaacat tcctattaga agaaccagcc catgaaaata gcggactgat caagacagac ttcctgggga aagggcaaca tactccgtca gttttcaaga tactcgagcc tg acctgagcgg cgacccttac gggagcagca gttctacctg tctggaagcc ccagcacatc atggagagtc aattggcgtg tctggaatgc cgatgattgg atnctccaca tggtttgacc atcttcttgg cccttcccca gccacgagag <210> 747 <211> 433 <212> DNA <213> Eucalyptus grandis <400> 747 tggacaaggc ctctggctcc acacgcaaat caagctcgtc atteggacgg gtcgacctgg acccttatat cctccacacc tctatctctg gttcgacccg tacacgtcgt gtactttgtc cgggggtccc ctacccaaag ggcttcaagt ccc gg caact gacgagatag aacttgtaca acagctgatt gatgggattc agccagccca ccaagaacca ccgcgggcac acttggagtt gccaaggcaa tccacactta c aat aaggga tgaccctcta gtacctctat tgtcaccacc cttggggaac aggcaatagg ctccgtcctt gttcaagaac ctcgacccta ggcaaattcg ttatacttac ttgagcggcg gagcagcaat tggaaccctt ttggatgcgg tgggacgctq 120 180 240 300 360 420 <210> 748 <211> 525 <212> DNA <213> Eucalyptus grandis <400> 748 WO 00/22092 PCT/NZ99/00I 69 aacagagcga tcttcttcaa ggtcttgcgt ggctgggcta tactctggcg atcaagctcg ggaccgaacc ctggtacaga tggttcgacc cttcagagct tggctgtccc tgttggtggg tggaccattt ctgggttcgg tcgagggcga acaacgaat t ccaacgtgta ccaccactga cgtgaagaaa ggtcttttcc tctggtcgcg tgtctacgaa gtcgaagagc ctcggctggg cgacttcgag cgtgaacggg Cttccactcc agcttttgct aaagtgtctg qgtgcgaggt ggagaggttc aagtacatgt accgncactg ttcctgggca gtgggcaacc tactccgtcc ctcgctcttc tgtcgttcgg ttgaggagct tcaagctcaa tcggcaaagt ccttctacat acacgacagg gggagcagag tctgg ttcttcttct cctgttcgtg ctaccagccg gcttgacaac taccatccag gtcgtcggat ggagccctac gctcggcctc 120 180 240 300 360 420 480 525 <210> 749 <211> 385 <212> DNA <213> Eucalyptus grandis <400> 749 cgtgagcttg ctgctgatgg cgacctgacg tggggtggca gctgtccctc gacagagtgt ccggatcgac atgcagctca ctacttgtct tcgcaagggc gagcggcgac ccttacatcc gcagcagttc tacctgtggg ctgccgcttc gcgaccgggc Cggggt cggg agctcgtcgc caactcacga tccacaccaa ttgac gcccgcagct caagatcttc Cttccggtcc cgggaactcc cgagatcgac cgtcttcact gggaacttct agcgggggtc aagaaggagt gccggcaccg ttcgagttcc caagggaagg accaggactt agctcctgtc acctgttcgg tgaccgctta tggggaacct gcaacaggga <210> 750 <211> 519 <212> DNA <213> Eucalyptus grandis <400> 750 caaacaccca tatcatctca aacaaaacac ttctcctggt acataacctg ccctcgacaa t tgacatgca tatcttcaaa gcgatccata cagctcttcc t tggcat t tg gccccatatg cgtttcttgg gggagatggc ggcctccggc actcaagctc cgggtcgacg cattctccac tctctctccc catctcttca gcctctcgtt gcagcatccg cgagctcaga tccggcttcc gttcccggca tgggacgaga accaacgtct tcagtgtctc gcaactacag ccacttcttc Cttttgcccg tcctcaatag agtccaagaa actccgcagg tagacttcga tcagccaag ctcttcagtc ccgcagcagc gctttgtacc caacttctac tggcgacctc cgagtacctg caccgtcacc gttcttgggg ctcactctca agcagcaaaa accgcgcttc caagacttcg ctcactcttt tttggcaaga gcttactatt aact'tgagcg 120 180 240 300 360 420 480 519 <210> 751 <211> 342 <212> DNA <213> Eucalyptus grandis <400> 751 cyt9aqcttg ctgctgatgg cgacctgacg tggggtggca qctgtccctc gacagagtgt ccaatno~r Atr--t ctacttIgt ct tcgcaa gggc gagcggcgac ccttacatcc ctgccgcttc gcgaccgggc cggggtcgg CaaCtCacya tccacaccaa 9cccgcagct caagatcttc Cttccggtcc cgagatcgac cgtcttcact gggaacttct accaggactt agcgggggtc agctcctgtc aagaaggagt acctgttcgg I-c I. t&CCL-L a ttcgagttcc tggggaacct ca <210> 752 <211> 416 <212> DNA <213> Eucalyptus grandis WO 00/22092PC/Z9016 PCT/NZ99/00169 <400> 752 gagaaacaga gcgacttcag ttcttcttct tcttcaatgg gttcgtgggt cttgcgttgt ccagccgggc tgggctatgg tgacaactac tctggcgctg catccagatc aagctcgtcg gtcngatgga ccgaaccaca agctcgtgaa ctgtcccggt tggtgggtct accattttgt ggttcgggtc agggcgactc acgaattcga gaaaagcttt Cttttccaaa ggtcgcgggt ctacgaagga gaagagcaag ggctgggacc cttcgagttc tgctctcgct gtgtctgtgt gcgaggtttg gaggttctca tacatgttcg gtcactgctt ctgggcaaca cttcttcttc cgttcggcct aggagctcta agctcaagct gcaaagttac tctacatgtc cgaaca <210> 753 <211> 408 <212> DNA <213> Eucalyptus grandis <400> 753 agcgacttca gagctcgtga ttcttcaatg gctgtcccgg tcttgcgttg ttggtgggtc ctgggctatg gaccattttg ctctggcgct gggttcgggt caagctcgtc gagggcgact accgaaccac aacgaattcg agaaaagctt t ctt t tcc aa tggtcgcggg tctacgaagg cgaagagcaa cggctgggac acttcgagtt ttgctctcgc agtgtctgtg tgcgaggttt agaggttctc gtacatgttc cgtcactgct cctgggcaac tcttcttctt tcgttcggcc gaggagctct aagctcaagc ggcaaagtta ttctacatgt acgaacag cttcttcttc tgttcgtggg accagccggg ttgacaacta ccatccagat cgtcggatgg <210> 754 <211> 401 <212> DNA <213> Eucalyptus grandis <400> 754 gagaaacaga gcgacttcag ttctcttctt cttcaatggc ttcgtgggtc ttgcgttgtt cagccgggct gggctatgga gacaactact ctggcgctgg atccagatca agctcgtcga tcggatggac cgaaccacaa agctcgtgaa tgtcccggtc ggtgggtctg ccattttgtc gttcgggtcg gggcgactcg cgaattcgac gaaaagcttt ttttccaaag gtcgcgggtg tacgaaggag aagagcaagt gctgggaccg ttcgagttct tgctctcgct tgtctgtgtc cgaggtttga aggttctcaa acatgttcgg tcactgcttt g cttcttcttc gttcggcctg ggagctctac gctcaagctt caaagttacc ctacatgtcg <210> 755 <211> 403 <212> DNA <213> Eucalyptus grandis <400> 755 gcgacttcag agctcgtgaa tcttcaatgg ctgtcccggt cttgcgttgt tggtgggtct gggctatgga ccattttgtc ctggcgctgg gttcgggtcg cgaaccacaa cgaattcgac gaaaagcttt cttttccaaa ggtcgcgggt tacgaaggag aagagcaagt qgaC-q ttcgagttcc tgctctcgct gtgtctgtgt caaggtttga aggttctcaa acatgttcgg tLact.cL L tgggcacacg cttcttcttc cnttcggcct ggagctctac gctcaagctt caaagttacc CLLaCtq aca ttcttcttct gttcgtgggt cagccgggct gacaactact atccagatca L Cyy -jLq&C 120 180 240 300 403 <210> 756 <211> 414 <212> DNA <213> Eucalyptus grandis WO 00/22092 WO 0022092PCT/NZ99/0OI 69 <400> 756 gagaaacaga gcgacttcag gttcgtgggt cttgcgttgt ccagccgggc tgggctatg tgacaactac tctggcgctg catccagatc aagctcgtcg gtcggatgga ccgaaccaca agctcgtgaa ctgtcccggt tggtgggtct accattttgt ggttcgggtc agggcgactc acgaattcga gaaaagcttt cttttccaaa ggtcgcgggt ctacgaagga gaagagcaag ggctgggacc cttcgagttc tgctctcgct gtgtctgtgt gcgaggtttg gaggttctca tacatgttcg gtcactgctt ctggcaacac cttcttcttc cgttcggcct aggagctcta agctcaagct gcaaagttac tctacatgtc gaca 120 180 240 300 360 414 <210> 757 <211> 441 <212> DNA <213> Eucalyptus grandis <400> 757 tgaaaatata ctcgagcctc agacagactg gtcgaaagcg gcacctggtc cacgggctcg ccacatggaa aatcaatgag agtacttcat gatctacgac ccgaatgcaa gcgctcgcga gaaggtcgct cggcagtata catgacttgc tcgtggtgtc tggaatgccg cccttcacgg tcgtcctgcg ctcgatgcct tactgcgcgg ttctagaaaa tagttaatgc t atgattgggc catactacag agatcggacg atggccggag acggcaagag gaaaaagagg attctttcgt cacgagaggc gaagttccag gcccgcttcc gcggctcagg gttccctcaa gaaatcaacg ttcgtttcgc ggactgatca gccaccgcct tactctggat tgggtccaga ggcatcccag tgttcgaatq atgttcaagt 120 180 240 300 360 420 441 <210> <211> <212> <213> 758 499
DNA
Eucalyptus grandis <400> 758 gcgacttcag tcttcaatgg cttgcgttgt tgggctatgg tctggcgctg aagctcgtcg ccgaaccaca gtacagacca ttcgacccca agctcgtgaa ctgtcccggt tggtgggtct accattttgt ggttcgggtc agggcgactc acgaattcga acgtgtacgt ccactgact gaaaagcttt cttttccaaa ggtcgcgggt ctacgaagga gaagagcaag ggctgggacc cttcgagttc gaacggggtg tgctctcgct gtgtctgtgt gcgaggtttg gaggttctca tacatgttcg gtcactgctt ctgggcaaca ggcaaccggg cttcttcttc cgttcggcct aggagctcta agctcaagct g ca aagtt a c tctacatgtc cgacagggga aacagaggct ttcttcttct gttcgtgggt ccagccgggc tgacaactac catccagatc gtcggatgga gccctacctg cggcctctgg 120 180 240 300 360 420 480 499 <210> 759 <211> 340 <212> DNA <213> Eucalyptus grandis <400> 759 gctggcaggg aagagagata ggcatcctgt ggaccgactc tttaagagaa cggaggctat tacgCcccct acqtcqccaa attgagcacc aattaagctg tggcctctgg tcaaatcata gggaggccaa gttctctgat caacqatgtc ttcgatcctt ttttatgtgg ttcccctcta cttgtcctgc ccaagtcata ccgatgactt ccatcagtac acaatgtacc catcagagag aacctatgtc tttatacgcc cta cia acggctgcgc agttgacccg 120 180 z 44 300 340 <210> 760 <211> 350 <212> DNA <213> Eucalyptus grandis WO 00/22092PC/Z9016 PCT/NZ99/00169 <400> 760 tcttggtaga caacattcct tccccaagaa ccagcccatg cgagaggcgg actgatcaag agttccaggc caccgcctgc ccgcttccta ctctggatcc ggctcangtg ggtccagaag attagagttt aaaatatact acagactggt acctggtcca acatggaaaa tacttcatga tcaagaatgg cgagcctctg cgaaagcgcc cgggctcgtc tcaatgagct tctacgacta agagtcaatt gaatgccgat cttcacggca gtcctgcgag cgatgcctat ctgcgcggac ggcgtgccct gattgggcca tact a cagg a atcggacggc ggccggaggc <210> <211> <212> <213> 761 288
DNA
Eucalyptus grandis <400> 761 cttgtggacg agacaccgat ccgaaggacc agcccatggg cagggcggcc gcatcaagac ttcgagatcg acgcgtgcga tgcagcagcg ccggcaggga ccgcgtgcac cgtctacagc cgactggacc gtgcccggcg gaggcggtac accaacttgg tcgatatgga cacgccccct accatggcag tggtgggacg agcaccgggg catcccgtac acgccgacga ctgggccacc tcatcacgtc ctaccgtaac cggcagacaa cgccaagcgg agcccacg <210> 762 <211> 364 <212> DNA <213> Eucalyptus grandis <400> 762 ttgacttcga gttcctgggg tcactcaagg gaagggcaac atttccacac atactccgtc ttcctattag agttttcaag ccatgaaaat atactcgagc tcaagacaga ctggtcgaaa cctg aacctgagcg agggagcagc atctggaagc aatggagagt ctctggaatg tcgcccttca gcgaccctta agttctacct cccagcacat caattggcgt ccgatgattg cggcatacta catcctccac gtggtttgac catcttcttg gcccttcccc ggccacgaga caggaagttc accaacgtct cccaccagaa gtagacaaca aagaaccagc ggcggactga caggccactg <210> <211> <212> <213> 763 343
DNA
Eucalyptus grandis <400> 763 acgacagggg agccctacct gagcagaggc tcggctctgg ggaaccagcg ccaagtcgtg tggagcaccg gggcatcccg ggaacgccga cgactgggcc ccttcatcac gtcctaccgt ggtacagacc ttcgacccca tttcttgtgg tacccgaagg acccanggcg aacttcgaga aacgtgtacg ccactgactt acgagacacc accagcccat Sc cg cat ca a tcgacgcgtg tgaacggggt ccactcctac gatccgcgtg gggcgtctac gaccgactgg cga gggcaaccgg tccgtcctct cacaccaact agctcgatat acccacgccc <210> 764 <21 ,,3G <212> DNA <213> Eucalyptus grandis <400> 764 cgtcactgct ttctacatgt cgtcggatgg accgaaccac aacgaattcg acttcgagtt cctgggcaac acgacagggg agccctacct ggtacagacc aacgtgtacg tgaacggggt gggcaaccgg gagcagaggc tCggcCtctg gttcgacccc accactgact tccactccta WO 00/22092 WO 0022092PCTINZ99/00 169 ctccgtcctc tggaaccagc gccaagtcgt gtttcttgtg gacgagacac cgatccgcgt.
gcacaccaac ttggagcacc ggggcatccc gtacccgaag gaccagccca tgggcgtcta <210> 765 <211> 516 <212> DNA <213> Eucalyptus grandis <400> 765 gcgacttcag tcaatggctg gcgttgttgg gctatggacc ggcgctgggt ctcgtcgagg aaccacaacg cagaccaacg accccaccac agctcgtgaa tcccggtctt tgggtctggt attttgtcta tcgggtcgaa gcgactcggc aattcgactt tgtacgtgaa tgacttcact gaaaagcttt ttccaaagtg cgcgggtgcg cgaaggagag gagcaagtac tgggaccgtc cgagttcctg cggggtgggc cctactccgt tgctctcgct tctgtgtcgt aggtttgagg gttctcaagc atgttcggca actgccttct ggcaacacga aaccgggagc cctctg cttcttcttc tcggcctgtt agctctacca tcaagcttga aagttaccat acatgtcgtc caggggagcc agagctcggc ttcttcttct cgtgggtctt gccgggctgg caactactct.
ccagatcaag ggatggaccg ctacctggta ctctggttcg 120 180 240 300 360 420 480 516 <210> 766 <211> 349 <212> DNA <213> Eucalyptus grandis <400> 766 gtccctcgac agagtgtcgg gatcgacatg cagctcaagc cttgtcttcg caagggccaa cggcgaccct. tacatcctcc gcagttctac ctgtggtttg gccccagcac atcatcttct ggtcgggctt tcgtcgccgg ctcacgatga acaccaacgt accccaccag tggtagacaa ccggtccaag gaac t ccgc c gattgacttc cttcactcaa aaatttccac cattcctatt aaggagtacc ggcaccgtga gagttcctgg 9ggaagggca acatactccg agagtt tt c tgttcggccg ccgcttacta ggaacctgag acagggagca tcatctggaa <210> 767 <211> 479 <212> DNA <213> Eucalyptus grandis <400> 767 gcgggttcca aacgaagcag tgggaggcga ctcggccgga ggccggagag ggatgagctg tgatccagac caacgtgtac 99ttcgaccc gaccgaggac tgttcttcgt cgaccaggtg tcttccccaa cgagaagccc cccagg cggccttqay agataccgat gtggtcactg gacttcgagt.
aagaacggga ttccactcct ccggtcaggg atgtacctct aagaccgact tcgggtggtt cttactacat tcttggggaa cgggctcgcg attccatcct tgttcaagaa tctcgagcat ggaccaaggc cagcaigaag gtgtt cggag ccggagcgga cgagatgcgg ctggaacagc caacggcgag ctggaacgcc gccgt tcgtg ctcaagctcg aacggggcag cagccgtatc cacatgctct caccagattg gcgaacaact gacgactggg tccacctac 120 180 240 300 360 420 479 <210> <211> <212> <213> 768 371
DNA
Eucalyptus grandis <400> 768 gttcggcctg atcgaagtca atctcaccgt gaccgcttac tacttgtctt cgcaagggcc aactcacgat gagattgact tcgagttcct ggggaacctg agcggcgacc cttacatcct ccacaccaac gtcttcactc aagggaaggg caacagggag cagcagttct acctgtggtt WO 00/22092 WO 0022092PCT/NZ99/00 169 tgaccccacc agaaatttcc acacatactc cgtcatctgg aagccccagc acatcatctt cttggtaaca acatcaccca tctctctctc tctctccccc actctctctc aagccaaaat ataagccaaa gtataagcca aagtatagag caatcatggc ccatgaaggt ggaggtccta gtgcttcctc C <210> 769 <211> 368 <212> DNA <213> Eucalyptus grandis <400> 769 gttcggccgg atcgaagtca aactcacgat gagattgact ccacaccaac gtcttcactc tgaccccacc agaaatttcc cttggtagac aacatcaccc tataagccaa agtataagcc agtgcttc atctcaccgt tcgagttcct aagggaaggg acacatactc atctctctct aaagtataga gaccgcttac ggggaacctg caacagggag cgtcatctgg ctctctcccc gcaatcatgg tacttgtctt agcggcgacc cagcagttct aagccccagc cactctctct cccatgaagg cgcaagggcc cttacatcct acctgtggtt acatcatctt caagccaaaa tggaggtcct 120 180 240 300 360 368 <210> 770 <211> 342 <212> DNA <213> Eucalyptus grandis <400> 770 ccagtccaag aacgagtacc caactccgca ggcaccgtca gatagacttc gagttcttgg cttcagccaa ggcaagggta tgatttccac acctactcca actcccatca gagagttcaa tgtttggcaa ccgcttacta ggaacttgag accgagagca t cct ct ggaa gaacgcagag gattgacatg tttatcttca cggcgatcca gcaattctat tccacaacgc tccatcggcg caactcaagc tcgttcccgg aacgggtcga cgtgggacga tacattctcc acaccaacgt ctctggttcg acccaactgc atcatattct catggacggg tt <210> 771 <211> 580 <212> DNA <213> Eucalyptus grandis <400> 771 ctcctcttca cagcagcagc tcgcatcgcc cttctatcaa cgacctcctc gtacttgttc cgtcaccgca cttggggaac gqgtaaca ctccatcctc gtcctcactc agcagcagac actctgcttc gacttcgaca actctctccc ggcaagattg tactatttat ttgagcggcg gagcagcaat tggaatccac tcatatcatc agaacacgcc tcgtggtcgt t aa cctgggg tcgacaaggc acatgcagct cttcaaacgg atccatacat tctatctctg aacgcatcat tcattgagat c a atat ggcc ttcttggggg tgatggccga ctccggctcc caagctagtt gtcggcgtgg tctccacacc gttcgaccca attctcagtg ttgcttctct tccctttcta acgtttgctt gctcagatcc ggcttccagt cccggcaact gatgagatag aacgtcttca actgctgatt tcagcaacta cttcttcgct ccgcccgcaa tcaacaacgg ccaagaacga ccgctggcac acttcgagtt gccaaggcaa tccacaccta 120 180 240 300 360 420 480 540 580 <210> ?7 <.21 1> 407 <212> DNA <213> Pinus radiata <400> 772 agtagtgtgc ccttcacaac aattatgtcg ccagctgggg ctcagatcac atcaaacatt ccatggcggt cgaaagatga gctgctcctc aacaaacagt atggtgcggg gtttgagtcg WO 00/22092 PCT/NZ99/OOI 69 aaggggacat gctggcactg tttgaattct ggcgggaagg cattcctatg atttatttgg gcatttcagt atgcagataa agctggttgc cggtgattcc tcaccgcctt ttatctttct tctcagactg cagagcacga tgagatagac tggggaacaa gtcgggggaa ccctacartc ttcagaccaa tgtatttacg gtgagagaga gcaccgaata tacctctggt tcgaccccac caaggattac cggtgctctg gaacatgtcc caaattgcat ttttggt 180 240 300 360 407 <210> 773 <211> 403 <212> DNA <213> Pinus radiata <400> 773 tgacaatgga caagagttgc caagcaagag tatctatttg tgcaggcaca gtcactacct cttcgaattt ctgggcaacc tcaaggcctt ggtgggcgtn ccacacttac tccgtgctct attcgagtgt ttaagaacag agcttactct ccaagattga tttatctatc tgtccggaga agcagcaatt ggacatcaaa ggagacagag tgaccgctct tatccaaatc atctcaaggt tccttatatt ttacttgtgg ccaaattata tngggtaaag tcaggttgtg aagttggtac cccaaacacg ttgcacacta ttcgacccaa ttttctgtag t99 gtattcaatc ctggcaactc acgaaataga atgtctttgc ccctggattt acgggagtct <210> 774 <211> 400 <212> DNA <213> Pinus radiata <400> 774 tgacaatgga caagagttgc caagcaagag tatctatttg tgcaggcaca gtcactacct cttcgaattt ctgggcaacc tcaaggcctt ggtgggcgtg ccacacttac tccgtgctct ttcgagtgtt taagaacagg agcttactct ccaagattga tttatctatc tgtccggaga agcagcaat t ggacat caaa gagacagagt tgaccgctct tatccaaatc atctcaaggt tccttatatt ttacttgtgg ccaaattata tgggtaaagt tcaggttgtg aagttggtac cccaaacacg ttgcacacta ttcgacccaa tttctgtaga gtattcaatc ctggcaactc acgaaataga atgtctttgc ccctggattt cgggagtcta 120 180 240 300 360 400 <210> 775 <211> 384 <212> DNA <213> Pinus radiata <400> 775 gataactgtt gttgataata tggttttttg cttctgggta gggcgattga tcatgttatg ctggcgctgg cttttcttcc aactcgttcc cggcgactct attgcatgac gaattcgatt qcagact aactac. tcaa atggcttgtt tctaattgtg aacgagggag aaggcaacat gcgggcacgt tcgaattctt t99g taagaatgca cagagttcaa agctgttgaa acttgtttgg gactgcattt gggaaatgct gagttgctgc tgatatcttc gctgaagct c aaaagtaggg tatatgtctt tcgggtgagc ttcttcattc gagcccagct gacaactttt gcgcagatta ctgaggggac cttacattgt 120 180 240 300 360 384 <210> 776 <211> 345 <212> DNTA <213> Pinus radiata <400> 776 tgagaaacag cccatgaggg ggtcctcttc aatctggatg cagataactg ggctactcaa ggtgggcggc tgaagataaa ctggggccat tctcctttta tctccactta caaaggcttc aacattggtg caaacaaata cggattaaat ggagaaccaa gaggggttat taaaaatgga WO 00/22092 PCTINZ99/00 169 agtaagtggt gggacaggcc ctctcattct tcccttactc cattacaaag gcgaatgctc cgatgggtac atcggaacta tatcatctat gattactgca aggattcgac caggttttcc acttIcgccac ctgagtgtgc aggcctccgc ttctagttct trata 240 300 345 <210> 777 <211> 449 <212> DNA <213> Pinus radiata <400> 777 gttgggtagc ctgcttctta cttcgagccc gctggacaat aggggcacag gtcttctgag tgagccttac tattacctct aacgtatgca attctggctc agctgggcga atttccgggg attaagctcg gggactctgc attgtgcaga ggttcgaccc gataagctgt tttgcttctg cagatcatgt ctggctttgc ttccaggtga acgacgaatt cgaatatcta cacggcaga tgttaaaatg ggcatcccat tatgtatgag ttccaagaca Ct ctg caggc cgatttcgaa Ctccaacggc gcttctttga tgtgcacagt ggagagctgt acatatttgt acagttactg ttcttgggaa actggcaaaa gtatgcagag ttaatgatat tgaagcttaa ttggaaaagc Ctttttatat atgcttcggg ggg aa ca a cg 120 180 240 300 360 420 449 <210> 778 <211> 354 <212> DNA <213> Pinus radiata <400> 778 tattaatcca taattatgga tcgtcgagtc ttcttgtaac ggcaatggtc gtggtaaaat acttcaggtt gtgggtttca ataaagttgg tacctggcaa ggttccgaac acgacgaaat catgggcatg tgtttctgca ccttgacaat atctaagaat Ct Ctgc cgg C agactatgaa cCCctcCtct aatttctaca ggccaacaat gagtatctgt acagttactg tttctaggca ttcttttctt acgatgtaga tacagcttac ttgctaaaat CCtattatct acctgtctgg attaataacc tatcacatgg tCtggatcgc tgatatgcaa gtcgtctcaa agat <210> 779 <211> 392 <212> DNA <213> Pinus radiata <400> 779 ccggtctgtt gttgtgggaa gtttattgtt atattcgtgg agaggatttt aaagtgacgt catcgatctc atcctggatc gtttggactt gtaagcatga tgctttttat atgagctcgg ggggacagat caggacagcc ataggatggc tttgcttaaa gggcagatga agaattcagg acatcaaact acaaggagga ttatacagtc cgggaaaagg Ctctgtttct ccatgtcaaa ggcaggattt tgtggcgggt agtgcgagat ca aattggttca gcacgcccgg acaaggtcag gcctccaaga gattctgcag gaattggatt agagaatcga Catcatttgc ataacaactc atcagtacat ggacagtcac tcgagtttct 120 180 240 300 360 392 'Z±U0> 780 <211> 293 <212> DNA <213> Pinus rac9i~t-A <400> 780 Cgtttattca aaaggggttg gcaacagaga tgcagacttt cattcctatt cctttctgtg tgatgtgccc gtacggatat tttccaacaa tcaacccatg ggcgtatact cgtcaatatg cctcgtcaag accgattgga gccacgcacc acagcgcttt gaatcgccac tgagaaaaga gaacgcagac tttcatttIcc ttcttatggt tcgacccaac caagttgttt tctttgtgga ggagtcccat atcctcaaac gattgggcta ctcaaggggg acatacaaga att 120 180 240 293 WO 00/22092 PCT/NZ99/00I 69 <210> 781 <211> 451 <212> DNA <213> Pinus radiata <400> 781 ctccaatggc actggcaaca tttccattcc tattcttttc tccgattagg gtattcccca catgagggta tcctcttcaa gaagataaac tggagccatt aaaccaatac ggattaaatg ggacaggccc tctcattctt atcggaacta tatcatctat gggaacaacg tgtggaacca acaacgagag tctggaatgc ctccttttat gagaatcaag cccttactcc gcctacctga catttacctc caagcaagtt gctgggagtc agataactgg ctccacttac agggt ttat t attacaaagg a tggttcgacc gtattctttg ccatatccta gctactcaag agaaggttcg gagaatggaa cgaatgctcc ccaccgcaga tagacagtgt agaaacagcc gtgggcggct acatcgatgc gtaagtggtg cgatgggtgc 120 180 240 300 360 420 451 <210> 782 <211> 387 <212> DNA <213> Pinus radiata <400> 782 cactggctta gatacacagt caagaagggg gtcgtgtcat ctgctcgtga gctctcaatg gatcatgtca tgtcccaagg ggatttgctt cgaagagcac cccggggact ctgctggcac gacgaattcg actttgaatt ccagtcgaat gcactgcgct tgcgtccttc agagttgctc atacatcttc tgttactgca tttgggg cgggtcttga cttctttgta gacgatttct cagctcaagc ggaaaagcaa ttctatatgt ggatggcatt tctttgtaac tctaccccag ttgataacat atgtgcagat cttcccaagg cgtaggttgt gttgtgcaac ttgggctgtt ttctggtgca taagctcgtt cgatcagcat 120 180 240 300 360 387 <210> 783 <211> 401 <212> DNA <213> Pinus radiata <400> 783 cgtttattca aaaggggttg tgcagacttt cattcctatt tgatgtgccc gtacggatat tcaacccatg ggcgtatact cctcgtcaag accgattgga tgatgcctgt caatattcct cgcttctctt gatggaaagc gcaacagaga CCtttctgtg tttccaacaa cgtcaatatg gccacgcacc cgaaaacgag agaggctgaa acagcgcttt gaatcgccac tgagaaaaga gaacgcagac tttcatttcc ctgcgcttcg actgaatggg ttcttatggt caagttgttt ggagtcccat gattgggcta acatacaaga tggtgggatg t tcgacccaac tctttgtgga atcctcaaac ctcaaggggg atttcagcat agcctgctta <211> 370 <213> Pinus radiata <400> 784 cttcalgaCCa a tgtatttac ttcgacccca ccaaggatta tttttggtan atgaggtacc tacccattta accagccgat acccgagggg gtctggagaa gaattccacg tcgatgcctg gcggcggtgg 99gggaag ccattcctat aatccgggtg gaagatctat aaccgactgg tgaggcttct ggtgagaga9 gctgtgctct ttcaagaaca tcgagcctgt gccaaggcgc gctccgaaat agcaccgaat ggaacatgta gcaaggatct ggaatgctga ccttcatcgc eggtgtgcgc atacctctgg ccaaattgca gggcgtgagg tgactgggcc ctcctacagg aacgaagggg 120 180 240 300 360 370 WO 00/22092PC/Z90I6 PCT/NZ99/00169 <210> 785 <211> 241 <212> DNA <213> Pinus radiata <400> 785 atctactcca acggcactgg caaaagggaa caacgtattt acctctggtt cgaccccacg gcagatttcc attcctattc ttttctatgg aaccacaacc aagttgtttt ctttgtggat .120 agtgttccga ttcgggtatt ccccaacaac gagcqgctgg gagtcccata tccgaaaagc 180 cagccgctga gagtatcctc ctcaatctgg aatgcagccg actgggctac acaaggcggg 240 c 241 <210> 786 <211> 180 <212> DNA <213> Pinus radiata <400> 786 catctactcc aacggcactg gcaaaaggga acaacgtatt tacctctggt tcgaccccac ggcagatttc cattcctatt cttttctatg gaaccacaac caagttgttt tctttgtgga 120 tagtgttccg attcgggtat tccccaacaa cgagcggctg ggagtcccat atcccgaaaa 180 <210> 787 <211> 264 <212> DNA <213> Pinus radiata <400> 787 ctggactttg agtttctggg gaacagaagt ggagagccct atgctctgca gacgaacatc tatgcaaagg gtgtgggtgg cagggaacag aggcacatcc tctggttcga tccaacaaca 120 cagtttcaca cttactccat cctctggaac tctcatcaga ttgtgttctt cgtagaccaa 180 gttcctgtga gagtccacag gcacactgag gctacgagcg atgcgttccc taaagaacag 240 gggatgtaca tgttttccag catt 264 <210> 788 <211> 298 <212> DNA <213> Pinus radiata <400> 788 ttccattttc cgcagaattc aaagcatggg gcatcttctc tactctctgg gaaagccgat taactgggcg acgaggggcg ggctggaaaa gattgactgg agcaaggcgc cattcgttgc 120 ctcctacagg ggatttgaga tcgaatcctg ccagtacccg ggtaaagcga gctgcgtggt 180 taacaccagc aattggtggg aagggttgag ctacagcggc ctcaaaccaa atcaagcgag 240 gttatacaaa tgggtgagga cgaattacat gatttatgat tattgtaagg acacgccc 298 <210> 789 <211> 375 <212 DM <213> Pinu6 radiata <400> 789 tcgtaccagg agactctgca ggagttgtca ctgcttatta tatgtcttct gacacagaca tgaataggga cgagctagat tttgaatttc tagggaacag aagtggacag ccttatggtc 120 ttcagacgaa catctattca aatggtgtog gtggaaggga acagaggcat atcctctggt 180 tcgatccaac gacagagttt cacacttact ctatcctctg gaacgctcat cagattgtgt 240 WO 00/22092PC/Z9016 PCT/NZ99/00169 ttttcgtgga ccaagttcca ttgagagtcc acaggcacac taaggctaca cgccatgtgt tccctcgaaa gcaggggatg tacatgttct ccagcatttg gaatggagac aactgggcaa cgagaaggcg gcctt 300 360 375 <210> 790 <211> 442 <212> DNA <213> Pinus radiata <400> 790 tcgtaccagg tgaataggga ttcagacgaa tcgatccaac ttttcgtgga tccctcgaaa cgagaggcgg gaaattccat agactctgca cgagctagat catctattca gacagagttt ccaagtncca gcaggggatg cnttgagaag gggctcggtg ggagttgtca tttgaatttc aatggtgtgg cacacttact ttgagagtcc tacatgtnct acccaacctg ca ctgcttatta tagggaacag gtggaaggga ctatcctctg acaggcacac ccagcatttg ggcggctgct tatgtcttct aagtggacag acagaggcat gaacgctcat taaggctaca gaatggagac ccgttcgtat gacacagaca ccttatggtc atcctctggt cagattgtgt cgccatgtgt aactgggcaa cttcgtacaa 120 180 240 300 360 420 442 <210> 791 <211> 424 <212> DNA <213> Pinus radiata <400> 791 cctattcggg tgttttccaa tatgggcgta tactcttcaa aaagaccgat tggagccacg ttgcaaatat agccctggca acttaccgca aagcagcgca ttactgcaaa gatccagtta gtctttaaac ttgacttcct gaat caatgagaaa tatggaacgc ctccttttat gttcatgtac tgcaactcaa gatttggtac acccattcca agaggagtcc agatgactgg ttccacatac ttcgtggtgg gtgggtacac acctccagca tgatcttaat catttcctca gctactcaag acaagtttca gatcagccgg gagaaataca gagtgtactg tcacaaatat aacccgccct gtggccgcgt acatcgatgc cgtacgcctc tgatttacga catgatgtgg ttgtccactt <210> 792 <211> 219 <212> DNA <213> Pinus radiata <400> 792 ggcacatgat gcatttatat gtcttctgag gggacttgca tgacgattcg atttcgaatt cttgggaaat gcttcgggtg agccttacat tcqtgcagact aatatctact ccaacggcac tggcgacagg gaacaacgca tttacctctg gttcgacccc accgcagatt tccattccta ttcttttctg tggaaccaca agcaagttgt attctttgt <210> 793 <211> 405 <212> DNA <213> Pinus radiata <400> 793 attcggcaga gctctggggt ccatttggct cgacaggaac gctacttcag tgcagccatt tttatttatc gaacaacgag tgggaaatat cccgggaaga atgcgggacg agggaggatt ccncagcacc tcgggaagcg aagttgcagg gctcacccgg ccttatacct attacaggaa aaacaatctg gatttaaatc ctgggtatac actaccacga tacagaccaa gggngcagca gaatgatcag gctgagaccq tgcaggagtc tgaaatagac catttatgta aatccacctt tctggaataa tacaagtctg aatactgcct attgaatttc agagctggaa tggtttgatc WO 00/22092PCNZ9016 PCT/NZ99/00169 ccactaagga ttttcaccgt tacagcattc tctggactcc atttg <210> 794 <211> 348 <212> DNA <213> Pinus radiata 405 <400> 794 ggaatgggga gagctgggca catttgttgc tcagttcaga ggttctgcaa gggaggcagc acgctgggga tagactcaaa gtaatgacaa ggtcagattc tagcctgccg agcatttttt accaacggag aacttttaca acacaaaatt ctccattggg aaagtagccc agtatttggt gaaagacaaa tcgatggct9 ggtggaataa taaggaagca ctgaggaatg aataagacag gatcacctgg gagccttacc tgagtggaat ggaaacccta gagaacatat gcttacttta ttatctcgtc tatgactact caggtaccac atttaatcca tctggtgg <210> 795 <211> 377 <212> DNA <213> Pinus radiata <400> 795 Ctttctctgg gaatcgccac tttccaacaa tgagaaaaga cttcaatatg gaacgcagat gccacgctcc ttttatttcc ctggtagttc atgtacttcg agatcatgca actcaagtgg cagttagatt tggtaca caatctgttt ggagtcccat gactgggcta acatacacaa tggtgggatc gtacacgaga tctttgtgga ttcctcaaac ctcaaggtgg gtttcaacat agccggcgta aatacatgat cgatgtacct ccgccctatg ccgcgtaaag cgatgcttgc cgcctcactt ttacgattac attcgggtgt ggcgtatact accgat tgga aaatacagcc accgcaaagc tgcaaagatt 120 180 240 300 360 377 <210> 796 <211> 379 <212> DNA <213> Pinus radiata <400> 796 gacatggctt gtttaagatt gtatctaatt gtgcagagtt atgaacgagg gagagctgtt tccaaggcca catacttgtt tctgcgggca cagtgactgc gatttcgaat tcttgggaaa ctccaaacgg gaactggcg gcagagctgc caatgatatc gaagctgaag tggaaaagta attttatatg tgcttcgggt tgctttttcg ttcgagccca ctcgacaatt ggggcgcaga tcttctgagg gaagccttac ttctggtttt gctgggcgat tttctggcgc ttaaactcgt ggacattgca attgtgcaga ttgcttctgg tgatcatgtt tggcttttct tcccggcgac tgacgaattc ctaatatcta <210> 797 <211> 315 <212> DNA <213> Pinus radiata <400> 797 atgtaagagc tggaaargcg ggacgaggga acctttggtt tgatcccact aaggattttc agattatatt ttttgtggat gacatcccaa ctttcccggc aaggcccatg tgggctgtac tacagataac gggaa ggattattac accgttacag taagaaagta ggatcgattc t L ta C 0' aggaagggag catt ct Ctgg tcgaaggacg tgggatgcat auuLdudULL cagcaaatcc actccattga aacccttaca CtCcttgggc <210> 798 WO 00/22092PC/Z9016 PCT/NZ99/00169 <211> 412 <212> DNA <213> Pinus radiata <400> 798 ggaaactttg atgatgattt aatggacatg gcgtgcagat aaacaatatt tgtttggcaa ggcactgttg tggctgttta gaatttttgg gcaatgttga ggctacgatg atcgagagca acatacacaa tcttttggaa ttacattatg tagtatggac gttcgaaatg tttgtattcc tggcaaggac gcggatcaaa ccgttaccac tggtcacctg aggcagtcag caaatcaagc aatcagccaa atcatcatgc Ctctggttgg attgtatttc atcatgtcac gttcgggctt ttccaccagg acagagacga agactaatgt atcccacagc tggtagatgg caggctctac cgcaacgaag aaat tctgcg gatcgacatt ttttgctaat agattttcac ct <210> 799 <211> 303 <212> DNA <213> Pinus radiata <400> 799 ggcagagcta gattttgaat ttctagggaa cagaagtgga cagccttatg gtcttcagac gaacatctat tcaaatggtg tgggtggaag ggaacagagg catatcctct ggttcgatcc aacgacagag tttcacactt actctatcct ctggaacgct catcagattg tgtttttcgt ggaccaagtt ccattgagag tccacaggca cactaaggct acacgccatg tgttccctcg aaagcagggg atgtacatgt tctccagcat ttggaatgga gacaactggg caacgagagg C9g <210> 800 <211> 298 <212> DNA <213> Pinus radiata <400> 800 cgtggaccaa gttccattgt gtgtccacag tcgaaagcag gggatgtaca tgttctccag aggcggcctt gagaagacca actgggcggc ccatggcctc ggctgcaaat gggaggatca taataatgca agtgcaaggc actggtggga gcacactaag catttggaat tgctccgttc aaacacgacc taagcccgag gcta ca cg cc ggagacaact gtatcttcgt caatcgtcct gcgcggactc atgtgttccc gggcaacgag acaagaaatt gtgctcacag tgacgaag <210> <211> <212> <213> 801 268
DNA
Pinus radiata <400> 801 tgaagctcaa gcgcgtacca ggagactctg caggagttgt cactgcttat tatatgtctt ctgacacaga catgaatagg gacgagctag attttgaatt tctagggaac agaagtggac aqccLtaL9q tcLtcagacg aacatccatt caaatogtgt gggtggaagg gaacagaggc atatcctctg gttcgatcca acgacagagt ttcacactta ctctatcctc tggaacgcnc atcagattgt gtttttcgtg gaccaagt <210.>82 <211> 395 <212> DNA <213> Pinus radiata <400> 802 cagagtttca cacttactct atcctctgga acgctcatca gattgtgttt ttcgtggacc WO 00/22092 PCT/NZ99/00I 69 aagttccatt aggggatgta t tgagaagac tcggctgcaa caagtgcaag aatattacag gagagtccac catgttctcc caactgggcg atgggaggat gcactggtgg gtgggtgaac aggcacacta agcatttgga gctgctccgt caaaacacga gataagcccg agcaaatatt aggctacacg ccatgtgttc cctcgaaagc atggagacaa ctgggcaacg agaggcggcc tcgtatcttc gtacaagaaa ttccatggcc cccaatcgtc ctgtgctcac agtaataatg aggcgcggac tctgacgaag aaacagaggg tgact 120 180 240 300 360 395 <210> <211> <212> <213> 803 429
DNA
Pinus radiata <400> 803 gttgggtagc agcgtagtgc actgcttctt aattctggct tcttcgagcc cagctgggcg agctggacaa tatttccggg caggggcaca gattaagctc tgtcttctga ggggactctg gtgagcctta cattgtgcag acgtattta agataagctg Ctttgcttct acagatcatg gctggctttg gttccaggtg cacgacgaat accgaatatc ttgttaaaat gggcatccca ttatgtatga cttacaagac aCtCtgcagg tcgatttcga taactccaac ggcttctttg ttgtgcacag gggagagctg aacatatttg cacagttact attcttgggg ggcactgggc agtatgcaga tttaatgata ttgaagctta t ttggaaaag gctttttata aatgcttcgg aaaagggaca 120 180 240 300 360 420 429 <210> <211> <212> <213> 804 432
DNA
Pinus radiata <400> 804 gttgggtagc agcgttgtgc actgcttctt aattctggct tcttcgagcc cagctgggcg agctggacaa tatttccggg caggggcaca gattaagctc tgtcttctga ggggactctg gtgagcctta cattgtgcag gtatttacct ct agataagctg ctttgcttct acagatcatg gctggctttg gttccaggtg cacgacgaat acgaatatct ttgttaaaat gggcatccca ttatgtatga cttccaagac actctgcagg tcgat t tcga actccaacgg ggcttctttg ttgtgcacag gggagagctg aacatatttg cacagttact attcttggga cactggcaaa agtatgcaga tttaatgata ttgaagctta tttggaaaag gctttttata aatgcttcgg aggggacaac 120 180 240 300 360 420 432 <210> <211> <212> <213> 805 438
DNA
Pinus radiata <400> 805 cgggggtcct gttcgagtgt attatcatta tcccaaaacc attgggcaac caggggtggg ccctccgcaa tttcaatgct ctttggaatc gaaccaagaa tctacqatta ttgtgcagac gattcatttc tatttaca ttnaagaaca caagcaatga cttgtgaaga gccgctactt cagaggcaga accaagagat gggagacaga gggtctactc ccgactggac cttcttttga ggctccagtg tcccccaggg gttgggtaaa cagcctttgg taaagctccc tgccgccgca ggtacgaaag accgcctcco L t. a ucUL L U gtgganagca aatgcagatg tttgttgcat gaggaggtgg aactacatga gaatgcaaat 120 180 240 300 360 438 <210> 806 <211> 393 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/0oI 69 <400> 806 accatattgt gttttctgta agaggrgttc catttccgaa gataactggg cgacgagggg gcctcctaca ggggatttga gttaacacca gcaattggtg aggttataca aatgggtgag aggtatccag tgctgcccac agatgatatc gaatcaaagc cgggctggaa gatcgaatcc ggaagggttg gacgaattac ttgagtgcaa ~cccat taggg atggggatct aagattgact tgccagtacc agctacagcg atgatttatg cgc tttacaagaa tcccaanncn ggagcaaggc cgggtaaagc gcctcaaacc attattgtaa caacgaagcg ctgggaagcc gccattcgtt gagctgcgtg agatcaagcg ggacacgccc 120 180 240 300 360 393 <210> 807 <211> 259 <212> DNA <213> Pinus radiata <400> 807 cacagtttga atggatatct gagagctgtt gaagcttaag catatttgtt tggaaaagca cagttactgc tttttatatg tcttgggaaa tgcttcggg ttcgagccca gctgggcgac agatcatgtt atgtatgagg ctggacaata tttccggggc tggctttgct tccaagacaa ggggcacaga ttaagctcgt tccaggtgac tctgcaggca tcttctgagg ggactctgca cgacgaattc gatttcgaat <210> 808 <211> 440 <212> DNA <213> Pinus radiata <400> 808 gcagtttctt tgtggatgat tcccatatcc tgaaactcaa gggctactca agggggcctc acaagaattt cagcattgat gggatgagcc tgcttacgct acgagaaata catgacttac cagagtgtga atgaaatgcc ctttgtattg catgcattcc gtgcccgcac cccatgggcg gtcaagaccg gcctgtcaat tctcttgatg gattactgca cttcttaaac ggatattttc tatactcgtc attggagcca attcctcgaa gaaagcagag aagattctgt ttgagctctc caacaatgag aatatggaac cgcacctttc aacgagctgc gctgaaactg cagatttccc atcaagatcc aaaagaggag gcagacgatt atttccacat gtttcgtggt aaatgggtac acgcgtccag cccttcattt 120 180 240 300 360 420 440 <210> <211> <212> <213> 809 263
DNA
Pinus radiata <400> 809 gaagccatga agaggacaca ttttcttgtt ctgtttcnca ttctgttact ccattctgct gccatggcta ccactcccag gaagccagtg agtgtgccct ttcacaacaa ttatgtcgcc agctggggct cagatcacat caaacaattc catggcggtc gaaagactga gctgctcctc aacaaacagt atggtgcggg gtttgantcg aaggggacat atttatttgg gcatttcagt atgcagataa agctggttgc cgg <210> 810 -Z 1 123 <212> DNA <213> Pinus radiata <400> 810 aaatgacaaa taaactgttg ttaaaatggc ttctttgagt atgcagagct gcttcttaat tctggtctct ttgcttctgg gcatcccatt gtgcacagtt taatgatatc ttcgagccca gctgggcgac agatcatgtt atgtatgagg gagagctgtt gaagcttaag ctggacaata 120 180 240 263 WO 00/22092 PCT/NZ99/00 169 tttccggggc tggctttgct tccaagacaa catatttgtt tggaaaagca ggggcacaga ttaagctcgt tccaggtgac tctgcaggca cagttactgc tttttatatg tcttctgagg ggactctgca cgacgaattc gatttcgaat ccttgggaaa tgcttcgggt gagccttaca ttgtgcagac gaatatctac tccaacggca ctggcaaaag ggacaacgta ttaacctctg ggt 240 300 360 420 423 <210> <211> <212> <213> 811 483
DNA
Pinus radiata <400> 811 gtcaaaagca gatcaatatg taccccacct ttctgatcat a ca gg tac tg aagatggttc t ctgaacatg ctccaaacta ttt tttctctttt gtgcccaatg ccgaagcctg atcaagtaca gcttccaatc ctggtgactc atgaaataga atgttttcag ggatagctag tactgttatt tggatgtgcc ttaatggggg caagggtagc tgcaggcgtt ctttgagttc tggaggaagg caggaggctg tctcttggta attccaaaaa gaacgaagcg tacttgtttg gtgactgcct ttgggcaata ggggcaagag gtgcttttct gcggcaatgg aactatgtac cactttctct gacatttcag tttatttatc ggtctggaca agcaacgcga aggcaatgct ctgctactgc cc acc tggg c tgacaaatgg tatgcagata ctctcagaac accttacatt atatccctgg 120 180 240 300 360 420 480 483 <210> <211> <212> <213> 812 323
DNA
Pinus radiata <400> 812 tgacaatgga caagcaagag ctgcaggcac acttcgaatt Ctcaaggcct tccacactta caagagttgc tatctatttg agtcactacc tctgggcaac tggtgggcgt ctccgtgctc agcttactct tgaccgctct ccaaagattg atatccaaat ttttatctat catctcaagg ctgtccggag atccttatat gagcagcaat tttacttgtg t99 tcaggttgtg caagttggta tcccaaacac tttgcacact gttcgaccca gtattcaatc cctggcaact gacgaaatag aatgtctttg accctggatt <210> 813 <211> 430 <212> DNA <213> Pinus radiata <400> 813 tgacaatgga caagagttgc caagcaagag taictattig tgcaggcaca gtcactacct cttcgaattt ctgggcaacc tcaaggcctt ggtgggcgtg ccacacttac tccgtgctct tattcgagtg ttaagaacag caagagcaag agcttactct ccaagattga tttatctatc tgtccggaga agcagcaatt ggacatcaaa ggagacagag tgaccgctct tatccaaatc atctcaaggt tccttatatt ttacttgtgg ccaaattata ttgggaaagt tcaggttgtg aagttggtac cccaaacacg ttgcacacta ttcgacccaa ttttctgtag ggaaacaatt gtattcaatc ctggcaactc acgaaataga atgtctttgc ccctggattt acgggagtcc atcattatcc 120 180 240 300 360 420 430 <210> 82.4 <211> 331 <212> DNA <213> Pinus radiata <400> 814 gtttcgggcg ccagtgaagg tcgttggaag ccatgaagag gacacagttt cttgttctgt WO 00/22092 PCTINZ99/00I 69 ttctcattct gttactccat tctgctgcca tggctaccac tcccaggaag cCagtgagtg tgccctttca caacaattat gtcgccagct ggggctcaga tcacatcaaa caattccatg gcggtcgaaa gactgagctg ctcctcaaca aacagtatgg tgcggggttt gagtcgaagg ggacatattt atttgggcat ttcagtatgc agataaagct ggttgccggt gattccgctg gcactgtcac cgccttttat ctttcttctc a <210> <211> <212> <213> 815 257
DNA
Pinus radiata <400> 815 gcatttacct ctggttcgac cccaccgtag atttccattc ctattctttt ctgtggaacc acaagcaagt tgtattcttt gtagacagtg ttccgattag ggtattcccc aacaacgaga ggctgggagt cccatatcct aagaaacagc ccatganggn atcctcttca atctggaatg cagataactg ggctactcaa ggtgggcggc tnangataaa ctggagccat tctcctttna tctccactta caaaagg 120 180 240 257 <210> <211> <212> <213> 816 216
DNA
Pinus radiata <400> 816 accccaccaa ggattaccat tcctatgctg tgctctggaa catgtaccaa attgcatttt tggtagatga ggtaccaatc cgggtgttca agaacagcaa ggatctgggc gtgaggtacc catttaacca gccgatgaag atctattcga gcctgtggaa tgctgatgac tgggccaccc gagggggtct ggagaaaacc gactgggcca aggcgc <210> 817 <211> 393 <212> DNA <213> Pinus radiata <400> 817 gccagctggg gctcagatca ctcaagaaac agtatggtgc agtatgcaga taaagctggt tcttctcaga ctgcagagca gaaccctaca ttcttcagac atatacctct ggttcgaccc taccaaattg cattttttgg cat caaacaa ggggtttgag tgccggtgat cgatgagata caatgtattt ca cc a agga t tagatgaggt ttccatggcg tcgaagggga tccgctggca gactttgaat acgggcggga taccattcct acc gtcgaaagac catatttatt ctgtcaccgc tcttggggaa agggtgagag atgctgtgct tgagctgctc tgggcatttc cttttatctt caagtcgggg agagcaccga ctggaacatg 120 180 240 300 360 393 <210> 818 <211> 457 <212> DNA <213> Pinus radiata <400> 818 ctcaacaaae agtatggtg agtatgcaga taaagctggt tcttctcaga ctgcagagca gaaccctaca ttcttcagac atatacctct ggttcgaccc taccaaattg catttttggt ctgggcgtga ggacccattt 99gggg tgag tgccggtgat cgatgagata caatgtattt caccaaggat agatgaggta aaccagccga tcgaagggga tccgctggca gactttgaat acgggcggga taccattcct ccaatccggg tgaagat gtgaa catatttatt ctgtcaccgc t citggggaa agggtgagag atgctgtgct tgttcaagaa tqaccAu.u tgggcatttc cttttatctt caagtcgggg agagcaccga ctggaacatg cagcaaggat 120 180 240 300 360 420 457 WO 00/22092 PCTINZ99/00 169 <210> 211> <212> <213> 819 283
DNA
Pinus radiata <400> 819 cgantatacc tctggttcga ccccaccaag atgtaccaaa ttgcattttt ggtagatgag ggatctgggc gtgaggtacc catttaacca tgctgatgac tgggccaccc gagggggtct catcgcctcc tacagggaat tccacgtcga gattaccatt gtaccaatcc gccgatgaag ggagaaaacc tgcctgtgag cctatgctgt gctctggaac gggtgttcaa gacacagcaa atctattcga gcctgtggaa gactgggcca aggcgccctt gct <210> <211> <212> <213> 820 342
DNA
Pinus radiata <400> 820 cattcctatt cttttttgtg attagggtat tccccaacaa agggtatcct cttcaatctg ataaactgga gccattctcc caatacggat taaatggaga aggccctctc attcttccct gaaccacaag cgagaggctg gaatgcagat ttttatctcc atcgagaggg tactccatta caagttgtat ggagtcccat aactgggcta acttacaaaa gttattgaga caaaggcgat tctttgtaga atcctaagaa ctcaaggtgg ggttcgacat atggaagtaa gc cagtgttccg acagcccatq gcggctgaag cgatgcaaac gtggtgggac 120 180 240 300 342 <210> 821 <211> 316 <212> DNA <213> Pinus radiata <400> 821 cgaatatacc tctggttcga ccccaccaag atgtaccaaa ttgcattttt ggtagatgag gatctgggcg tgaggtaccc atttaaccag gctgatgact gggccacccg agggggtctg atcgcctcct acagggaatt ccacgtcgat tgcgctacgc aggggg gattaccatt gtaccaatcc ccgatgaaga gagaaaaccg gcctgtgagg cctatgctgt gggtgttcaa tctattcgag actgggccaa cttctgctcc gctctggaac gaacagcaag cctgtggaat ggcgcccttc ggaatcggtg <210> <211> <212> <213> 822 460
DNA
Pinus radiata <400> 822 tgacaatgga caagagttgc Caa9caa9&q tatctatttg tgcaggcaca gtcactacct cttcgaattt ctgggcaacc tcaaoacctt aotoor1-n ccacacttac tccgtgctct tattcgagtg tttaagaaca tcccaagagc caagcaatga agcttactct ccaagattga tttatctatc tgtccggaga ggacatcaaa gggagacaga gcgtctactc tgaccgctct tatccaaatc atctcaaggt tccttatatt ccaaactara gttgggtaaa cagcctttgg tcaggttgtg aagttggtac cccaaacacg ttgcacacta ttttctgtag gtggataaca gtattcaatc ctggcaactc acgaaataga atgtctttgc acgggagtcc attatcatta 120 180 240 .v u 360 420 460 <210> 823 <211> 329 <212> DNA WO 00/22092 PCT/NZ99/00 169 <213> Pinus radiata <400> 823 cgtctcaagg ccccaaacat atccttatat tatgcacact tttacttgtg gttcgaccca accaaatcat attctctgta agttgggtaa agtggatagc caagcctctg gaatgcagat gacgaaatag actttgaatt aatgtcttcg ctcaaggcct accttggatt tccacactta gacgagactc ccgttcgagt aattatcact atcctaagag gattgggcg tct tggcaac tggcaatcgt ctccgtgctc gtttaagaac ccaagcaatg ctctctgggg gagcaacaat tggacatcaa agggagacag aaggtctatt 120 180 240 300 329 <210> 824 <211> 328 <212> DNA <213> Pinus radiata <400> 824 ctttctctgg aatcgccacc ttccaacaat gagaaaagag ttcaatatgg aacgcagatg ccacgctcct tttatttcca tggtagttca tgtacttcgt gatcatgcaa ctcaagtggg aatctgtttt gagtcccatt actgggctac catacacaag ggtgggatca t a cacgag ctttgtggac tcctcaaacc tcaaggtggc tttcaacatc gccggcgtac gatgtaccta cgccctatgg cgcgtaaaga gatgcttgca gcctcactta ttcgggtgtt gcgtatactc ccgattggag aatacagccc ccgcaaagca 120 180 240 300 328 <210> 825 <211> 352 <212> DNA <213> Pinus radiata <400> 825 gcatttacct Ctggttcgac acaagcaagt tgtattcttt ggctgggagt cccatatcct cagataactg ggctactcaa tctccactta caaaaggttc gagggtttat tgagaatgga cccaccgcag gtagacagtg aagaaacagc ggtgggcggc gacatcgatg agtaagtggt atttccattc ttccgattag ccatgagggt tgaagataaa caaaccaata gggacaggcc ctattctttt ggtattcccc atcctcttca ctggagccat cggattaaat ctctcattct ctgtggaacc aacaacgaga atctggaatg tctcctttta ggagaatcaa tc 120 180 240 300 352 <210> 826 <211> 215 <212> DNA <213> Pinus radiata <400> 826 gccagctggg gctcagatca catcaaacaa ttccatggcg gtcgaaagac tgagctgctc ctcaacaaac agtatggtgc ggggtttgag tcgaagggga catatttatt tgggcatttc agtatgcaga taaagctggt tgccggtgat tccgctggca ctgtcaccgc ctttnatctt tcttctcaga ctgcagagca cgatgagatg acttt <210> 827 <211> 463 <212> DNA <400> 827 gttgacatgg cttgtttaag attgcagagc tgctgctttt tcgttctggt ttttttgctt ctgggtatct aattgtgcag agttcaatga tatcttcgag cccagctggg cgattgatca tgttatgaac gagggagagc tgttgaagct gaagcttggc cattttttct ggcgctggct tttcttccaa ggccacatac ttgtttggaa aagtaggggc gcagattaaa ctcgttcccg 120 180 240 WO 00/22092 PCT/NZ99/00 169 gcgactctgc gggcacagtg actgcatttt atatgtcttc tgaggggaca ttgcatgacg aattcgattt cgaattcttg ggaaatgctt cgggtgagcc ttacattgtg cagactaata tctactccaa cggcactggc gacagggaac aacgcattta cctctggttc gaccccaccg cagatttcca ttcctattct tttctgtgga accacaagca agt <210> 828 <211> 342 <212> DNA <213> Pinus radiata 300 360 420 463 <400> 828 caacttttct ggcgctggct gcagattaaa ctcgttcccg tgaggggaca ttgcatgacg ttacattgtg cagactaata cctctggttc gaccccaccg agttgtattc tttgtagaca tttcttccaa gcgactctgc aattcgattt tctactccaa cagatttcca gtgttccgat ggcaacatac gggcacagtg cgaattcttg tggcactggc ttcctattct tagggtattc ttgtttggaa actgcatttt ggaaatgctt aacagggaac tttctgtgga cc aagtaggggc atatgtcttc cgggtgagcc aacgcattta accacaagca 120 180 240 300 342 <210> 829 <211> 447 <212> DNA <213> Pinus radiata <400> 829 ccggtctgtt gtttattgtt agaggatttt catcgatctc gtttggactt tgctttttat ggggaacaga tggccgcgag gttgtgggta atattcgtgg aaagtgacgt atcctggatc gtaagcatga atgagc tcgg tcaggccagc cagagagtga ataggatggc tttgcttaaa gggcagatga agaattcagg acatcaaact acaaggagga cttatacagt atctctg cgggcaaagg ctctgtttct ccatgtcaaa ggcaggattt tgtggcgggt agtgcgagat ccaaacaaat aattggttca gcacgcccgg acaaggtcag gcctccaaga gattctgcag gaattggatt gtgtttgctc agagaatcga catcatttgc ataacaactc atcagtacat ggacagtcac tcgagtttct tcgggaaggg 120 180 240 300 360 420 447 <210> 830 <211> 471 <212> DNA <213> Pinus radiata <400> 830 tgtttaagaa tgtgcagagt ggagagctgt acatacttgt acagtgactg ttcttgggaa actggcaaca tattcttttc tgcagagttg tcaatgatat tgaagctgaa ttggaaaagt cattttatat atgcttcggg gggaacaacg tgtggaacca ctgcttcttc cttcgagccc gctcgacaac aggggcgcag gtct t ctgag tgagccttac catttacctc caagcaagtt attctggttt agctgggcga ttttctggcg attaaactcg gggacattgc attgtgcaga tggttcgacc gtattctttg tttgcttctg ttgatcatgt ctggcttttc ttcccggcga atgacgaatt ctaatatcta ccaccgcaga tagacagtgt ggtatctaat tatgaacgag ttccaaggca ctctgcgggc cgatttcgaa ct cc aatgg c tttccattcc t <210> 831 <212> DNA <213> Pinus radiata <400> 831 taggatggcc gggcaaagga attggttcaa gagaatcgag tttattgtta tattcgtggt ttgcttaaac tctgtttctg cacgcccggc atcatttgca gaggatttta aagtgacgtg ggcagatgac catgtcaaaa caaggtcaga taacaactcc atcgatctca tcctggatca WO 00/22092 PCT/NZ99/00 169 gaattcaggg gcaggatttg cctccaagaa tcagtacatg tttggacttg taagcatgaa catcaaactt gtggcgggtg attctgcagg gacagtcact gctttttata tgagctcgga caaggaggaa gtgcgagatg aattggattt cgagtttctg gggaacagat caggccagcc ttatacagtc caaacaaatg tgtttgctct c <210> 832 <211> 304 <212> DNA <213> Pinus radiata <400> 832 catcccctgC tttcgaggga acacatggcg tggaacttgg tccacgaaaa acacaatctg aaactctgtc gttggatcga accagaggat tgaatagatg ttcgtctgaa gaccataagg atctagctcg tccctattca tgtctgtgtc tgc a tgtagcctta gtgtgcctgt atgagcgttc cagaggatag atgcctctgt tcccttccac ctgtccactt ctgttcccta agaagacata taataagcag ggactctcaa agtaagtgtg ccacaccatt gaaattcaaa tgacaactcc 120 180 240 300 304 <210> 833 <211> 234 <212> DNA <213> Pinus radiata <400> 833 acccatttaa ccagccgatg aagatctatt cgagcctgtg gaatgctgat gactgggcca cccgaggggg tctggagaaa accgactggg ccaaggcgcc cttcatcgcc tcctacaggg aattccacgt cgatgcctgt gaggcttctg ctccgcaatc ggtgtgcgct acgcaggggc ggnggtggtg ggatcaggag gagttcagag acctggatgg gcggcaatgg cggt <210> 834 <211> 375 <212> DNA <213> Pinus radiata <400> 834 ggagcattcg cctttgtaat ttccattttt aataacccct tgaagccttt gtaagtggag cttgagtagc ccagttatct taggatatgg gactcccagc caaagaatac aacttgcttg ggtcgaacca gaggt ggagtaaggg cttggttctc ataaaaggag gcattccaga Ct ctcgt tgt tggttccaca aagaatgaga catttaatcc aatggctcca t tgaagagga tggggaatac gaaaagaata gggcctgtcc gtatttgttt gtttatcttc taccctcatg cctaatcgga ggaatggaaa caccacttac gcatcaatgt agccgcccac ggctgtttct acactgtcta tctgcggtgg <210> <211> <212> <213> 835 352
DNA
Pinus radiata <400> 835 t ggacatttc agtatgc aga cttttattta tcctctcaga taggtctgga Caaccttaca agagcaacgc gtatatctct ttggaatatg catcagattg taaagatggt actctgaaca ttctccaaac ggtttgaccc tattctttgt Lcctggtgac tgatgaaata taatgttttc cacaaaagac ggatgatgtc tctgcaggcg gactttgagt agtggaggaa tatcattcct cccatcagag ttgtgactgc tcttgggcaa agggggacag acactgtcct tt <210> 836 WO 00/22092 WO 0022092PCTINZ99/001 69 <211> 368 <212> DNA <213> Pinus radiata <400> 836 ctataagtcc ggattcttca cattgcagca ctctatctct cattgagttc ctggggacaa aaatggaaca ggggatgggc tgacccaact gaagacttcc ctatgtagat gatattgcta gaggccac gtgctgctat ccaataacca caccaggaaa aggttctcac ataactacag tccgaaagta taagctacag ggagtaccca accctacacc aggcagggag ccttctctgg cccaagaaga gcaggttata ggtcaccatg ttacagacca t tgaagt tt c actccaagtt atttcatcta cagctggagt acgaaataga atgtttacat atctctggtt atatcatctt cttatccatt <210> 837 <211> 402 <212> DNA <213> Pinus radiata <400> 837 ggagcattcg cctttgtaat ttccattttt aataacccct tgaagccttt gtaagtggag cttgagtagc ccagttatct taggatatgg gactcccagc caaagaatac aacttgcttg ggtcgaacca gaggtaaatg ggagtaaggg cttggttctc ataaaaggag gcattccaga ctctcgttgt tggttccaca cgt tgttccc aagaatgaga catttaatcc aatggctcca ttgaagagga tggggaatac gaaaagaata tgtcgccagt gggcctgtcc gtatttgttt gtttatcttc taccctcatq cctaatcgga ggaatggaaa gc caccacttac gcatcaatgt agccgcccac ggctgtttct acactgtcta tctgcggtgg 120 180 240 300 360 402 <210> 838 <211> 389 <212> DNA <213> Pinus radiata <400> 838 cacagactcg ttgggtgctt aatgctgatc aatatggtgc tactgctacc tcacctccga ctgggcttct gatcatatca caaatggaca ggtactggct gcagataaag atggttcctg tcagaactct gaacatgatq actgaaagat ccaatgtact agcctgtgga agtacattaa tccaatccaa gtgactctgc aaatagact agctagcagg gttatttctc tgtgccattc tggggggaac gggtagctac aggcgttgtg aggctggtgc ttggtagcgg caaaaaaact gaagcgcagc ttgtttggac actgcctttt ttttctagac caatggctgc atgtacccac tttctcttga atttcagtat atttatcctc 120 180 240 300 360 389 <210> <211> <212> <213> 839 451
DNA
Pinus radiata <400> 839 ggatttagga gtgaggtatc tgctgatgac tgggctacaa ttgtccaact ttaggccgtc gcaat.ggagg aaactgaagt cagagtgagg tatcctaaaa cagcctcctt gggatagata ctaattatgt tataactgcc cattcaacca ggggtgggttgatggtggga gggttcgtoa tgtctccaga gctatatttt atttcgtagt gcccatgaaa ggagaagaca t cagaaagcc taggtacacc gtgtaccana tat tctattc a atctattcaa gactggagca ttcgatgacc atatacaact gaccgtgaca ttcttcgaca gcttgtggaa aggcaccctt ttgatggaca actgcactga tctaatagca tatggctgtt 120 240 300 420 451 <210> 840 WO 00/22092 WO 0022092PCT/NZ99OOI 69 <211> 459 <212> DNA <213> Pinus radiata <400> 840 gaataatttc aggtagtgga ctgctattaa gctacaggca ataaccagga gtacccaggt caggaaaacc ctacacctta ttctcacagg cagggagttg actacagcct tctctggact gaaagtaccc aagaagaatt caatatggga tgcttcctct ttcaagtctt ggt tat acag caccatgacq cagaccaatg aagtttcatc ccaagttata tcatctactt tgggctactg tggaggccta ctggagtcat aaatagacat tttacataaa tctggtttga tcatcttcta atccattgag aaaatggca taagtccgga tgcagcactc tgagttcctg tggaacaggg cccaactgaa tgtagatgat gccactttgg ttcttcagtg tatctctcca gggacaacac gatgggcagg gacttccata attgctatcc gtatatggat <210> 841 <211> 476 <212> DNA <213> Pinus radiata <400> 841 tttttttttt gaatcatcaa gattgatacc ctctaatatt tagaaatgaa tcagaatact gattgaggtt ttatttgcat aataatcgta gatcatgtag tcgattccaa agccacctcc aattgcggaa ggatgcaaca tggttgccca atcatctgca aagaatctcg ttacatgtgt tggaaggaaa tcgggaggcg ttctttcgta tctgcggcgg aagggagctt ttccaaaggc cttttgtatt gtctgagaaa gaattcagag gtccctgggg cccactgtag cgtcaaaaga tagtccagtc tggagtagac accagtaccc gaatgaaaga ttcattctct gaatctcttg cctctgcctc agaagtagcg ggtcttcaca cctcattgct taaatgaatt attaatgtaa gaattcaaag gtgtctgcac tgttcttgct gcagcat tga agcccacccc ttgggt 120 180 240 300 360 420 476 <210> 842 <211> 293 <212> DNA <213> Pinus radiata <400> 842 ggctgatcat ggcagtaatc gtaagtcaaa ctctgtttct tcgtcagagt ccgcgcctcg ttattactgt gagcacagga cgattgggtc ccatggaatt tcttgtacga agatacgaac ccgcctctcg ttgcccagtt gtctccattc tatttgctgt ggcttatccc gtgttttgat ggagcagccg caaatgctgg tcacccacct accagtgcct cctcccattt cccagttggt agaacatgta gtaatattcc tgcacttgca gcagccgagg Cttctcaagg cat 120 180 240 293 <210> 843 <211> 460 <212> DNA <213> Pinus radiata <400> 843 gtgttgattg gtctgattgc aaggtttttg atgataattt aacaagtata gattcggatg ggagttgtca ctgcttatta tttgaatttc tagggaacag aatggtlgtgg gtggaaggga tacacttact ctatcctctg ctgtgtttct tcagatattg qqt. L. L 0 gttcagcatg tatgtcttct aagtggacag acagaggcat gaacgct cat tgccagtccg tgggctcagg aagctcaagc gacacagaca ccttatggtc atcctctggt cagattgtgt acggtgatga atcacttcag tcgtaccagg tgaataggga ttcagacgaa tcgatccanc ttcatctccc gacctctgaa agactctgca cgagctagat catctattca gacagagttt 120 180V 240 300 360 420 460 <210> 844 WO 00/22092 WO 0022092PCT/NZ99/O01 69 <211> 491 <212> DNA <213> Pinus radiata <400> 844 tggtgactct gcaggcgttg tgaaatagac tttgagttct tgttttcagt ggaggaaagg aaaagactat cattcctaca tgatgtcccc atcagagttt ccagcccatg aaaatctatt gttggagaag acagactgga ggatgggtgt gaggcgtcta gggatcaaga a tgactgcctt tgggcaatag gggacagaga ctgtcctttg tcaagaacag caagcttgtg gcaaggcacc tgcctcactc ttatttatcc gtctggacaa gcaacgcgta gaatatgcat caaggattta gaatgctgat ctttgttgca tgcttgtcca tctcagaact ccttacattc tatctctggt cagattgtat ggagtgaggt gactgggcta tcatacaagg actttaggcc ctgaacatga tccaaactaa ttgaccccac tctttgtgga atccattcaa caaggggtgg gattccacgt cgtcgatggt 120 180 240 300 360 420 480 491 <210> 845 <211> 413 <212> DNA <213> Pinus radiata <400> 845 gtttgcacag actcgttggg taggcaatgc tgattaatat gctgctactg ctaccccacc cccacctggg cttctgatca cttgacaaat ggacaggtac agtatgcaga taaagatggt tcctctcaga actctgaaca tgcttactga ggtgcccaat tccgaagcct tatcaagtac tggcttccaa tcctggtgac tgatgaaata aagatagcta gtactgttat gtggatgtgc attaatgggg tccaagggta tctgcaggcg gactttgagt gcaggaggc t ttCtcttggt cattccaaaa ggaacgaagc gctacttgtt ttgtgactgc tcttgggcaa ggtgcttttc agcggcaatg aaactatgta gcagctttct tgga cat tt c cttttattta tag <210> 846 <211> 513 <212> DNA <213> Pinus radiata <400> 846 gggagacaga ggg tc tact c ccgactggac cttcttttga ggctccagtg tcccccaggg aactctgaat ttctcagaca acaaaagcga gttgggtaaa cagcctttgg taaagctccc tgccgtcgca ggtacgaaag accgcctccc tctaccttcc cacatgtaaa gattcttttg gtggatagca aatgcagatg tttgttgcat gaggaggtgg aactacatga gattgcaaat aagtattctg atattagagg atgaaaaaaa attatcatta attgggcaac ccctccgcaa ctttggaatc tctacgatta aaaacctcaa attcatttct gtatcaatca aaa tcccaaaacc caggggtggg tttcaatgct gaaccaagaa ttgtgcagac tcctttgaat attacattaa tttcattttg caagcaatga cttgtgaaga gccgctactt cagaggcaga accaagagat tcagagaatg ttctcattct gtactgctat 120 180 240 300 360 420 480 513 <210> 847 <211> 362 <212> DNA <213> Pinus radiata <400> 847 ctacacctta cagaccaatg cagggagttg aagtttcatc tctctggact ccaagttata aagaagaatt tcatctactt tgcttcctct tgggctactg tgttgctaag ttctctaagt tttacataaa tctggtttga tcatcttcta atccattgag aaaatggcaa tcattctcag tggaacaggg cccaactgaa tgtagatgat gccactttgg atacagagca tggctgccct gacgggcagg gacttccata attgctatcc gtatatggat gattacagat gtttcagact ttctcacagg actacagcct gaaagtaccc caatatggga atcagccatt ccacatgctc WO 00/22092 PCT/NZ99/00I 69 362 <210> 848 <211> 417 <212> DNA <213> Pinus radiata <400> 848 ctcgtttctg gaactgctgc gacatcgaca tcgacatcga gcagctcttg cttctgcaaa gctaaaatca ttgacaatgg ggtattcaat ccaagcaaga cctggcaact ctgcaggcac gacgaaatag acttcgaatt tctgcatcac cacacctctt tttctacaat acaagagttg gtatctattt agtcactacc tctgggcaac aaactctttt cacctcctct gatgtcgaca cagcttactc gccaagattg ttttatctat ctgtccggag cccaatccct ttcttttatt ttacatgggg ttgaccgctc atatccaaat catctcaagg atccttatat aatcgacatc ggcctcctcg taatgatcgt ttcaggttgt caagttggta tcccaaacac tttgcac 120 180 240 300 360 417 <210> 849 <211> 291 <212> DNA <213> Pinus radiata <400> 849 atattatgca cactaatgtc ttcgctcaag tgtggttcga cccaaccttg gatttccaca tcatattctc tgtagacgag actcccgttc gtaaagtgga tagcaattat cactatccta tctggaatgc agatgattgg gcgactagag gccttggtaa cttactccgt gagtgtttaa agagccaagc gtggactcgt tcgtgagcaa gctctggaca gaacagggag aatgaaggtc caagacagac caattt tact tcaaaccaaa acagagttgg tattcaagcc t 120 180 240 291 <210> 850 <211> 299 <212> DNA <213> Pinus radiata <400> 850 actgtcccaa ggggagccgc tccagctcaa ttccaagcac acatacattt tcggaaaagt ctctgctggc accggtattg ctttctatat tgactttgaa tttttgggta acatttctgg ttcaaaaggc agtggcaata gggagcaacg gctcgatccc gaatgggcaa gtcttcccaa acagccatac catgttcttg gcttctggtg attaaactcg ggggacgaac actgtgcaga tggtttgacc cagggtttgc ttcctggaga acgatgaatt ccaatgttta caactgtag 120 180 240 299 <210> 851 <211> 359 <212> DNA <213> Pinus radiata <400> 851 gtgctcagta gtctgattgc aaggtttttg atgataattt aatggccaag tatggcacct ggagttgtca CLgCt.LaLta tttgagtttc tggggaacag tagtgtttgt tcagataatq ggttcttgac tatgtctcct aagtggagag tcccagtccc tgggctgaa 9 cagaactcag aacaccgaca ccatatgctc aaggggatga atcatttcag gttctgggtt tctccq tgaataggga tgcagacaaa ttcatctgct gacctctgaa caagtccaag aqactcLqca cgagctggac catctatgc 120 2480 300 3S9 <210> 852 <211> 347 <212> DNA <213> Pinus radiata WO 00/22092 WO 0022092PCT/NZ99/0OI 69 <400> 852 gtggaattge ttgtaggag ccctcgggtg gcccagttat gaaagggaac cttatcccca cacaaaaaaa ctgcacaaaa caaattttga aaagtttttt ttgtgaattt ttcagaataa cggtgaaggg ccgcattcca ggttcttgca aacccatcac tggtttttta aaatatttaa cgccttgctc caggctcgaa gttcttgaac acacatcaat ggattttatt aaaattttca caatcggttt tagatcttca acgcggattg attcatgaac tgtttagaat aatatct tctccagacc ttggttggtt gtaccgagtc gttccagtgc tttggaagga <210> 853 <211> 434 <212> DNA <213> Pinus radiata <400> 853 ggtgttcaag aacagcaagg ctattcgagc ctgtggaatg ctgggccaag gcgcccttca ttctgctccg caatcggtgt cagagacctg gatgggcggc ctacaattac tgcactgaca tcgcgacaat atgtagtcca cgtggtcgga taat atctgggcgt ctgatgactg tcgcctccta gcgctacgca aatggcggta cgcccagaaa gttatcctca gaggtaccca ggccacccga cagggaattc ggggcggcgg cttgaaatgg caagcaaatg tcatttgatg tttaaccagc gggggtctgg cacgtcgatg tggtgggatc gtgaggaagc cctccggaat ctacccatgg cgatgaagat agaaaaccga cctgtgaggc aggaggagtt actacaccat gtgttcgcga ctgaataatc 120 180 240 300 360 420 434 <210> 854 <211> 274 <212> DNA <213> Pinus radiata <400> 854 attcaatcca agcaagagta tctatttgcc ggcaactctg caggcacagt cactactttt gaaatagact ttgaatttct gggaaacctg gtctttgctc aaggccttgg tggacgtgag ctggatttcc acacttactc cgtgctctgg aagattgata tccaaatgaa gttggtacct tatctatcat ctcaaggtcc caaacacgac tctggagatc cttatatttt gcacactaat cagcaatttt acttgtggtt cgacccaacc actt 120 180 240 274 <210> <211> <212> <213> 855 366
DNA
Pinus radiata <400> 855 ataagctgtt gttaaaatgg ttgcttctgg gcatcccatt agatcatgtt atgtatgagg tggctttgct tccaagacaa tccagytqac tccgcaggca cgacgaattc gatttcgaat gaatat cttctttgag gtgcacagtt gagagctgtt catatttgtt cagttactgc tcttgggaaa tatgcagagc taatgatatc gaagcttaag tggaaaagca tttttatatg tgcttcgggt tgCttcttaa ttcgagccca ctggacaata ggggcacaga tcttctgagg gagccttaca ttctggctct gctgggcgac tttccggggc ttaagctcgt ggactctgca t tgggcagac <211> 398 <212> DNA <213> Pinus radiata <400> 856 gtccagtcga atcgggtctn gaggatggca ttcgtaggtt gtcaagaagg gggtcgtgac WO 00/22092 WO 0022092PCT/NZ99/001 69 atgcactgng tgtgcgtnct ggagagttg9C acatacaict actgttactg tttttgggga ctcttctttg tngacgattt tccagctcaa t cggaaaagc cattctatat acacttttgg tatctttgta cttctacccc gcttlgataac aaatgtgcag gtcttcccaa ggagccgtac acgttgtgca acctgctcgt gagctctcaa agttgggctg ttgatcatgt catgtcccaa atttctggtg caggatttgc ttngaagagc ataaagctcg ttcccgggga ctctgctggc ggcgatcagc atgacgaatt cgactttgaa gctgtgca 120 180 240 300 360 398 <210> 857 <211> 183 <212> DNA <213> Pinus radiata <400> 857 cagtacatgt ttggacttgt aagcatgaac atcaaacttg tggcgggtga ttctgcaggg acagtcactg ctttttatat gagctcggac aaggaggaag tgcgagatga attggatttc gagtttctgg ggaacagatc aggccagcct tatacagtcc aaacaaatgt gtttgctctc ggg <210> 858 <211> 464 <212> DNA <213> Pinus radiata <400> 858 cagactcgtt gggtgcttac tgctgatcaa tatggtgccc ctgctacccc acctccgaag gggcttctga tcatatcaag aatggacagg tactggcttc agataaagat ggntcctggt agaactctga acatgatgaa acattctcca aactaatgtt tgaaagatag aatgtactgt cctgtggatg tacattaatg caatccaagg gactctgcag atagactttg ttcagtggag ct agcaggag tatttctctt tgccattcca gggggaacga gtagctactt gcgttgtgac agttcttggg gaaaggggga gctggtgctt ggtagcggca aaaaaactat agcgcagctt gtttggacat tgccttttat caataggtct caga ttctaggcaa atggctgcta gtacccacct tctcttgaca ttcagtatgc ttatcctctc ggacaacctt 120 180 240 300 360 420 464 <210> 859 <211> 412 <212> DNA <213> Pinus radiata <400> 859 tttacttgtg gttcgaccca accaaattat attttctgta agttgggtaa agtggatagc ccagcctttg gaatgcagat ctaaagctcc ctttgttgca atgccgccgc agaggaggtg gggtacgaaa gaactacatg accctggatt gacgggagtc aattatcatt gattgggcaa tccctccgca gctttggaat atctacgatt tccacactta ctgttcgagt atcccaaaac ccaggggtgg atttcaatgc cgaaccaaga attgtgcaga ctccgtgctc tggacttcaa gtttaagaac agggagacag ccaagcaatg agggtctact gcttgtgaag accgactgga tgccgctact tcttcttttg acagaggcag aggctccagt caccaagaga tt 120 180 240 300 360 412 <210> 860 <211> 376 <212> DNA <213> Pin-us radiata <400> 860 acgatgagat agactttgaa ttcttgggga acaagtcggg ggaaccctac attcttcaga ccaatgtatt tacgggcggg aagggtgaga gagagcaccg aatatacctc tggttcgacc ccaccaagga ttaccattcc tatgctgtgc tctggaacat gtaccaaatt gcatttttgg tagatgaggt accaatccgg gtgttcaaga acagcaagga tctgggcgtg aggtacccat WO 00/22092 WO 0022092PCT/NZ99/0OI 69 ttaaccagcc gatgaagatc tattcgagcc tgtggaatgc tgatgactgg gccacccgag ggggtctgga gaaaaccgac tgggccaagg cgcccttcat cgcctcctac agggaattcc acgtcgatgc ctgtga <210> 861 <211> 536 <212> DNA <213> Pinus radiata <400> 861 gacatggctt gtatctaatt atgaacgagg tccaaggcca tctgcgggca gatttcgaat tccaacggca ttccattcct ccgattagg gtttaagatt gtgcagagtt gagagctgtt catacttgtt cagtgactgc tcttgggaaa ctggcgacag attcttttct tattccccaa gcagagctgc caatgatatc gaagctgaag tggaaaagta attttatatg tgcttcgggt ggaacaacgc gtggaaccac caacgagagg tgctttttcg ttcgagccca ctcgacaatt ggggcgcaga tcttctgagg gagccttaca atttacctct aagcaagttg ctgggagtcc ttctggtttt gctgggcgat tttctggcgc ttaaactcgt ggacattgca ttgtgcagac ggttcgaccc tattctttgt catatcctaa ttgcttctgg tgatcatgtt tggcttttct tcccggcgac tgacgaattc taatatctac caccgcagat agacagtgtt gaaaca 120 180 240 300 360 420 480 536 <210> 862 <211> 358 <212> DNA <213> Pinus radiata <400> 862 gctatatctc ccacagttta aataacgctt gggtattgaa ggttcgggat ttcaatcgta ctggtgcccg gaaattccgc gggcacgacg aaattgatat catacaaaca tttatgtcaa tgcagacgtc caatggccag taaggagttt cggtaccgtt ggagttccta tggttcagcc tacagtgata ggattgcagc ttgtttggaa acgacatatt ggaaatgttt cgataaagag ttgatgtaac tttctcttac gcgt tgatat atctatcttc ctggggagcc cagcagttct atggggcgaa caattattct ctccatgaaa aacaggtgag ctacattctg atttatgg <210> 863 <211> 322 <212> DNA <213> Pinus radiata <400> 863 acgatgagat agactttgaa ccaatgtatt tacgggcggg ccaccaagga ttaccattcc tagatgaggt accaatccgg ttaaccagcc gatgaagatc ggggtctgga gaaaaccgac ttcttgggga aagggtgaga tatgctgtgc gtgttcaaga tattcgagcc tg acaagtcggg gagagcaccg tctggaacat acagcaagga tgtggaatgc ggaaccctac aatatacctc gtaccaaatt tctgggcgtg tgatgactgg attcttcaga tggttcgacc gcatttttgg aggtacccat gccacccgag <210> 864 <211> 372 <212> DNA <400> 864 tccatttccg cgtgcccggc tgatcaggtc tgtaacatcg atgtgctcgg aagcgatgaa tctactactc cattctgctg ccatggctgc caaaaattac ggtgcaagct ggggctcaga ggtcgaactt cttctcaaca aacagtatgg ttcatttttc gactgcccaa cacgcccaag ccacatcaaa tgcggggttc tgcaaaataa tttctgggtc ccagtgagtg gaattccatg gaatccaagg tttcattttg tgtttctcat tgcccttcgg gaggtcgaaa ggacatattt WO 00/22092 WO 0022092PCT/NZ99/0O1 69 gtttgggcat ttcagcatgc agattaagtt ggttcctggt gactcggctg gcactgtcac ggccttctat ct ':210> <211> <212> <213> 865 519
DNA
Pinus radiata <400> 865 ataatggctt gtatctaatt atgaacgagg tccaaggcaa tctgcgggca gatttcgaat t cc aa cgg ca ttccattcct ccgattaggg gtttaagaat gtgcagagt t gagagctgtt catacttgtt cagtgactgc tcttgggaaa ctggcaacag attcttttct tattccccaa gcagagttgc caatgatatc gaagctgaag tggaaaagta attttatatg tgcttcgggt ggaacaacgc gtggaaccac caacgagagg tgcttcttca ttcgagccca ctcgacaact ggggcgcaga tcttctgagg gagcct ta ca atttacctct aagcaagttg ctgggagtc ttctggtttt gctgggcgat tttctggcgc ttaaactcgt ggacattgca ttgtgcagac ggttcgaccc tattctttgt ttgcttctgg tgatcatgtt t gg ct ttt ct tcccggcgac tgacgaattc taatatctac caccgcagat agacagtgtt 120 180 240 300 360 420 480 519 <210> 866 <211> 240 <212> DNA <213> Pinus radiata <400> 866 ctctcatcac agggttcgaa ccgcgacgag ctggatttcg agtttctggg gaataaatct ggagatccta ccattcttca aactaatgta tacgcaaatq gaaaaggcga ccgcgagcag cgaatatacc tctggtttga tccatccact gaattccaca cctaccgtgt tatctggaac gctgcgtata tcttatttat ggtggatgag gtgcctgtta gagtttttat gaacaacaac <210> 867 <211> 392 <212> DNA <213> Pinus radiata 120 180 240 <400> 867 tgtttgcaga gatttgttgg ctcttggtag ctgcaatggc ttcccaaaaa attatgta~c aatgaggttc agctttctct tacttgtttg gacactttag gtgactgcct tttatctatc ctgggcaata ggtctggaca ggcaatgctg tgctatggct cacttgggct agacaaatgg tatgcagata ctcccagaat gccttacatt at caagatgg gcctcacctc gCtgatcata acaggtactg aagatggttc gccgagcacg ct tccccaaact ctaagcctgt tcaagtacat gcttccaatc ctggtgactc atgaaataga gttgctggtg ggacgtacca caatggtgga caagggtacc tgcaggcact ttttgagttc 120 180 240 300 360 392 <210> <211> <212> <213> 868 605
DNA
Pinus radiata e-4flO AAR cqtqcccggc tgatcaggtc atgtgctcgg aagcgatgaa cattctgctg ccatggctgc ggtgcaagct ggggctcaga cttctcaaca aacagtatgg ttcagcatgc agattaagtt ctttcttctc aaactgcaga ttCaLttLtc gactgcccaa cacgcccaag ccacatcaaa tgcggggttc ggttcctggt gcacgacgag tgcaaaataa tttctgggtc ccagtgagtg gaattccatg gaatccaagg gactcggctg atagatttcg tttcattttq tgtttctcat tgcccttcgg gaggtcoaaa ggacatattt gcactgtcac aattttt 9 9 9 cgcaacaccg tctactactc caaaaattac ggtcgaactt gtttgggcat ggccttctat caacaggtct 6 Q 120 180 240 300 360 420 WO 00/22092PC/Z9016 PCT/NZ99/00169 ggacaacctt acattcttca gaccaatgta ttcacaggag gcaagggtga gagagagcat cgcatatatc tctggttcga tcccaccaag gattaccatt cctatgcagt actctggaac atgtaccaga tcgtgttttt tgtggactcg gtaccaatcc gcgtgttcaa gaactgcaag gacct <210> 869 <211> 528 <212> DNA <213> Pinus radiata <400> 869 atgaattcga tttcgaattc atatctactc cggeagattt acagtgttcc aacagcccat ggcggctgaa tcgatgcaaa agtggtggga gggtggatcg caacggcact ccattcctat gattagggta gagggtatcc gataaactgg ccaatacgga ca gg ccct ct gaactatatc ttgggaaatg ggcaacaggg tcttttttgt ttccccaaca tcttcaatct agccattctc ttaaatggag cattcttccc atctatgact cttcgggtga aacaacgcat ggaaccacaa acgagaggct ggaatgcaga cttttatctc aatcgagagg ttactccatt actgcaagga gccttacatt ttacctctgg gcaagttgta gggagtccca taactgggct Cacttacaaa ggttattgag acaaaggcga ttcgacca gtgcagacta ttcgacccca ttctttgtag tatcctaaga aCtcaaggtg aggttcgaca aatggaagta atgctccgat 120 180 240 300 360 420 480 528 <210> 870 <211> 277 <212> DNA <213> Pinus radiata <400> 870 ggaacatgta ccagatcgtt ttttttgtgg gcaagaacct ggggataagg ttccctttca ggaatgcgga taactgggcc acccgagggg ccttcaccgc ctcctacaag caattccacg cggtgtgcgc tacgcagggg cggcggtggt actcggtacc aatccgcgtg ttcaagaact accaaccaat gaagatctat tcgagcctgt gtctggagaa aaccgattgg agcaaggcgc tcgatgcctg cgaagcttct gtttcggagt gggatca 120 180 240 277 <210> 871 <211> 501 <212> DNA <213> Pinus radiata <400> 871 caacaattat gactgagctg atttgggcat cgccttttat gaacaagtcg gagagagcac gctctggaac gaacagcaag cctgtggaat gtcgccagct Ctcctcaaca ttcagtatgc CtttCttctc gggg aaccc t cgaatatacc atgtaccaaa gatctgggcg gctgatgact ggggctcaga aacagtatgg agataaagct agactgcaga acattcttca tCtggttcga ttqcattttt tgaggtaccc tcacatcaaa tgcggggttt ggttgccggt gcacgatgag gaccaatgta ccccaccaag gtqtaoataaa atttaaccag caattccatg gagtcgaagg gattccgctg atagactttg tttacgggcg gattaccatt gtaccaatcc ccgatgaaga gcggtcgaaa ggacatattt gcactgtcac aattcttggg ggaagggtga cctatgctgt aoatattcaa tctattcgag <21u> <211> <212> <213> 872 540
DNA
Pinus radiata <400> 872 gcgattctat tctaagcaga ccagggattc gattggagcc ttcagcatta tctgctaaat ttgatcgata tCgtttgcat ctatggaggt ttctagggct ttaagttatg tccatttcct WO 00/22092 WO 0022092PCT/NZ99/001 69 tttgattgcc tattgatgta gctttctctt aagcgttgat ctatttatct ttccgggcag gcagcagttc attatctgca acatggggcg accaattatt atctccatga t ct acaggt g ccttacattc tatttatggt cagttatatc aaaacaacgc Ctggttcggg aactggtgcc aggggcacga tgcacacaaa tcgatccaac tcccacagtt ttgggtattg atttcaatcg cggaaattcc cgaaattgat catttatgtc ttcagatttc tatgcagacg aacaatggcc tataaggagt gctggtaccg atggagtttt aatggctcag cacaattact tctacagtga agggattgca ttttgtttgg ttacgacata taggaaatgt ccaataaaga ccattctctg 180 240 300 360 420 480 540 <210> <211> <212> <213> 873 397
DNA
Pinus radiata <400> 873 gcccggctga tcaggtcttc tgctcggaag cgatgaagac tctgctgcca tggctgccac gcaagctggg gctcagacca ctcaacaaac agtatggtgc agcatgcaaa ttaagttggt tcttctcaaa ctgcagagca at'ttttctgc tgcccaattt gcccaagcca catcaaagaa ggggttcgaa tcctggtgac cgacgagata aaaataattt ctgggtctgt gtgagtgtgc ttccatggag tccaagggga tcggctggca gatttcg cattttgtgt ttctcattct ccttcggcaa gtcgaaaggt catatttgtt ctgtcacggc aacatcgatg actactccat aaattacggt cgaacttctt tgggcatttc cttctatctt 120 180 240 300 360 397 <210> <211> <212> <213> 874 371
DNA
Pinus radiata <400> 874 ataagctgtt gttaaaatgg ttgcttctgg gcatcccatt agatcatgtt atgtatgagg tggctttgct tccaagacaa tccaggtgac tctgcaggca cgacgaattc gatttcgaat gaatatctac t cttctttgag gtgcacagtt gagagctgtt catatttgtt cagttactgc tcttgggaaa tatgcagaac taatgatatc gaagcttaag tggaaaagca tttttatatg tgcttcgggt tgcttcttaa ttcgagccca ctggacaata ggggcacaga tcttctgagg gagccttaca ttctggctct gctgggcgac tttcCggggc ttaagctcgt ggactctgca ttgtgcaaac <210> <211> <212> <213> 875 355
DNA
Pinus radiata <400> 875 Ctttgtagac gatgtgcccg tcctcaatct caaccaatgg tcaaggtggc ctcgtcaaga tttcagcatc gatgcctgtg gcctgcttac gcttcgctcg atacatgact tacga ttact tacgggtatt gcgtatactc ccgattggag attattcctt atgcaaagca 9caaagattt tgggaacaat ttcaatatgg ccacgcacct gaaaacgacc gaggctgaaa agccaggttt gagaaagtgg aacgcagacg ttcgtttcca tqctcttcgt ctgaagtggg cccacggctc gagtcccgta actgggctac catacacaaa qqtgoaatoa tgcaagagaa cgcca 120 180 240 300 355 <210> 876 <211> 337 <212> DNA <213> Pinus radiata <400> 876 gcagagattt gttgggtaag ggcaatgctg atcaagatgg tccccaaact gttgctggtg ctcttggtag ctgcaatggc tgctatggct gcctcacctc ctaagcctgt ggacgtacca 120 WO 00/22092 WO 0022092PCT/NZ99/00I 69 ttcccaaaaa attatgtacc cacttgggct gctgatcata tcaagtacat caatggtgga aatgaggttc agctttctct agacaaatgg acaggtactg gcttccaatc caagggtacc tacttgtttg gacactttag tatgcagata aagatggttc ctggtgactc tgcaggcact gtgactgcct tttatctatc ctcccagaat gccgagc <210> 877 <211> 558 <212> DNA <213> Pinus radiata <400> 877 gggtagcagc gtagtgcaga gcttcttaat tctggctctt tcgagcccag ctgggcgaca tggacaatat ttccggggct gggcacagat taagctcgtt cttctgaggg aactctgcac agccttacat tgttcagacg tttacctctg gttcgacccc accaagttgt tttctttgtg tgggagtccc atatccga taagctgttg tgcttctggg gatcatgtta ggct tt gct t ccaggtgact gacgaattcg aatatctact acggcagatt gatagtgttc ttaaaatggc catcccattg tgtatgaggg cc aag a caa c ctgcaggcac atttcgaatt ccaacggcac tccattccta cgattcgggt ttctttgagt tgcacagttt agagctgttg atatttgttt agttactgct cttgggaaat tggcaaaagg ttcttttcta attccccaac atgcagagct aatgatatct aagct taagc ggaaaagcag ttttatatgt gcttcgggtg gaacaacgta t gg aacc a ca aacgagcggc 120 180 240 300 360 420 480 540 558 <210> 878 <211> 400 <212> DNA <213> Pinus radiata <400> 878 aagcattaca gagataagtc aagcatatct aagaagaata cctaagaggc tgctattaaa cctcactttg tcagcgcagt tctccattgc attccatcaa agtagcacaa gtcgactcag aaacacctta atcttgtgac gattacagaa tgtcacggtc agttatagat ggtcatcgaa tacagaggcc ttcgttgctg tgcaaaacag gatatattaa tctggtacac ggtgtaactg ggccttttga tcacacccat attggcttta taacgatgac tatatctata tctggaggca ttacgaaccc tcccaccacc ttaatatttc ataaataaca aataagagcc ttgctggaga ccttcagttt gacgaccctg 120 180 240 300 360 400 <210> 879 <211> 500 <212> DNA <213> Pinus radiata <400> 879 agatccctat attatgcaca attctacttg tggttcgacc aaatcaaatt acattgtccg agaattggat aaaacagata ctccagcctt tggaatgcag gactaaagcc ccctttgttg Cttcgacgga gagga tgttt g~acatat a~ttacga agaatgcaaa tgagcgctct ccaatatctt caaccctggc tggacgggat gcaattatca atgattgggc catctttcag tggattcgaa actLqc&,ja cgctcaaggc ttttcacact tcctgttcgt ttatccgaag aaccagaggt caacttcagt cgaagagcag cttggcaatc tactccgtgc gtgtttaaaa agccaagcaa ggcctcgtca gctgcgactt aagcttcaat LLCuccucag gtgaacagca tctggacacc acagggagac tgaggatgta agacagactg cttcttctaa gggtgcgaaa 9yc~accugc <210> 880 <211> 547 <212> DNA <213> Pinus radiata WO 00/22092PC/Z9O16 PCT/NZ99/00169 <400> 880 atgctgtgca gcctggtagt agtgggctgc tgaccatgtt cttctggtgc aggatttgct tcaagctggt tcctggcgac gtgatcaaca tgacgaattc ttgtgcagac gaatgtttat ggttcgatcc aactgcgaat ttttctttgt ggacgatgtg catttcctca aacccgccct ctactca gggctctcaa atgtcccagg tcgaagagca tctgctggca gactttgaat tcaaatgggg ttccattcct cctattcggg atgggcgtat tgtgcagcct gcgatttgct catacttgtt ccgttactgc tcttggggaa ttggcaacag actcctttct tgttttccaa actcttcaat tcaacgatct ccagctcaag cggaaaagtg tttctatatg cacttcagga agagcagcgt ctggaatcgc caatgagaaa atggaacgca tttctacgcc ctcgatcaca aatgttcaga tcttcccaag gaaccgtacg atttacctgt caccaatctg agaggagtcc gatgactggg 120 180 240 300 360 420 480 540 S47 <210> <211> <212> <213> 881 197
DNA
Pinus radiata <400> 881 tctctagaca aatggacagg tactggcttc caatccaagg gtacctactt gtttggacac tttagtatgc agataaagat ggttcctggt gactctgcag gcactgtgac tgccttttat ctatcctccc agaatgccga gcacgatgaa atagattttg agttcctggg caataggtct ggacagcctt acattct <210> <211> <212> <213> 882 622
DNA
Pinus radiata <400> 882 catcctccta tctattcctt ctatttctat aataacctcg cacatggggc gaatcgcact tatgcaaatc gtctcaaggt tcctatatt ctacttgtgg tcaaattaca catacattcg tcatcccttg taacccataa tcgagtcttc gatggtcgtg tcaggttctg aagttggtac cccaagcacg atgcacacca ttcgacccaa ttgtccgtgg tttcactact cctacactaa ttatggacac ttgtaattgt gtaagatcct ggt tt Caat c ctgggaactc acgaaataga atatcttcgc ccctggcttt ac Ctttctttgc tctgcacttg aggaacgccc ttctgcaaat tgacaatggc caagaatgag tgccggcaca ctacgaattt tcaaggcctt tcacacttac acgcgtccat gatctcaaat ctcctctttc ttctacaacg caacaattac tatctctttg gttactgctt ctaggcaacc ggcaatcgtg tccgtgctct ctcgagctat tcaagttcct ttttcttatt atgtcgacat agcttactct ctaaagttga attatctgtc tctctggaga aacagcaatt ggacaccaaa 120 180 240 300 360 420 480 540 600 622 <210> 883 <211> 223 <212> DNA <213> Pinus radiata <400> 883 gctggtgctc ttgagctg cgtaccattc ccaaaaaatt yqLqycaLy ayyLL,- yL ggtacctact tgtttggaca caatggctgc tatggctgcc tcacctccta agcctgtgga atgtacccac ttgggctgct gatcatatca agtcatcaat LLLLdyaau adtyacag gcacrggcrt ccaacccaag ctttagtatg cagataaaga tgg 120 223 <210> 884 <211> 304 <212> DNA <213> Pinus radiata WO 00/22092PC/Z90I6 PCT/NZ99/00169 <400> 884 gagagcagag gatatacctc t.Ctggaacat gcatcagatc atagcaggga cttatgagtg tgtggaatgc tgatgactgg caccatttgt tgcatcatac agt c tggtttgacc gtattctttg aggtacccat gccacaaggg aggggattcc ccacaaaaga tggacgatgt ttaaccagcc gtgggctgga acgtggatgg ttaccatgcc ccccatcaga catgaaaata gaagacagat gtgtgaggcc tactctgtgc gttttcaaga tactcaagcc tggagcaaag tctgtaactg 120 180 240 300 304 <210> <211> <212> <213> 885 367
DNA
Pinus radiata <400> 885 aaggggtggg ctggagaaga attccacgtg gatgggtgtg tcggtggtgg gatcaaaagg gggggttcgt aacagttaca aatgcctcca gagtgtacca tttatagata tattaatata atgtcat cagattggag aggcctctgt Ccttcgatga ccatctataa gagaccgtga tcttctgtaa caaagcacca aactgagtcg ccttgatgga ctactgcgct catttaatag tctattcttc tttgttgcat acttgtgcta atgcaatgga gacaaagtga cagcctctta ttagatatgc catacagggg ctcagggtcg gaaaactgaa ggtctccagc ggggctctta tttgttattt 120 180 240 300 360 367 <210> 886 <211> 358 <212> DNA <213> Pinus radiata <400> 886 gggccacaag gggtgggctg acaggggatt ccacgtggat agggtcgtcg gtggtgggat aactgaaggg ggttcgiaac ctccagcaat gcctccagag gctettattt atagatatat gagaagacag gggtgtgagg caaaaggcct agttacacca tgtaccagag taatatatct attggagcaa cctctgtaac tcgatgacct tctataacta accgtgacat tctgtaatct agcaccattt tgagtcgact tgatggaatg ctgcgctgac ttaatagcag attcttctta gttgcatcat tgtgctactc caatggagaa aaagtgaggt cctcttaggg gatatgct <210> 887 <211> 343 <212> DNA <213> Pinus radiata <400> 887 aaggggtggg ctggagaaga attccacgtg gatgggtgtg tcggtggtgg gatcaaaagg gggggttcgt aacagttaca aatgcctcca gagtgtacca tt'Qtagaa tattaatata cagattggag aggcctctgt ccttcgatga ccatctataa gagaccgtga tcttctgtaa caaagcacca aactgagtcg ccttgatgga ctactgcgct catttaatag tctattcttc tttgttgcat acttgtgcta atgcaatgga gacaaagtga cagcctctta tta catacagggg ctcagggtcg gaaaactgaa ggtctccagc ggggctctta 120 180 240 300 343 <210> 888 <211> 517 <212> DNA1 <213> Pinus radiata <400> 888 ctcctacatg tttgagcgat tttactcatt cgtttcatac tctttctttg cacgcgtcca tctcgagcta ttctattcct ctcatccctt gcctacacta atctgcactt ggatctcaaa ttcaagttcc tctatttcta ttaatccatg attatggaca caggaacgcc cctcctcttt WO 00/22092 WO 0022092PCT/NZ99/00I 69 cttttcttat gatgtcgaca cagcttactc gctaaagttg tat tat ctgt ct CtCt ggag taataacctc tcacatgggg tggatcgcac atatgcaaat cgtctcaagg atccctatat gtcgagtctt cgat cgtcgt ttcaggttct caagttggta tcccaagcac tatgcacacc cttgtaattg ggtaaaatcc gggttccaat cctgggaact gacgaaatag aatatct tttctgcaaa tttctacaac ttgacaatgg ccaacaatta ccaagaatga gtatctcttt ctgccggcac agttactgct actacgaatt tctaggcaac 240 300 360 420 480 517 <210> 889 <211> 543 <212> DNA <213> Pinus radiata <400> 889 ctcgtgccgC ttcagtttcg tttcttgttc tgtttctcat aagccagtga gtgtgccctt aaacaattcc atggcggtcg tttgagtcga aggggacata ggtgattccg ctggcactgt gagatagact ttgaattctt gtatttacgg gcgggaaggg aaggattacc attcctatgc gag ggcgccagta tctgttactc tcacaacaat aaagactgag tttatttggg caccgccttt ggggaacaag tgagagagag tgtgCtctgg aggtcgttgg cattctgctq tatgtcgcca ctgctcctca catttcagta tatctttctt tcgggggaac caccgaatat aacatgtacc aagccatgaa ccatggctac gctggggctc acaaacagta tgcagataaa ctcagactgc cctacattct acctctggtt aaattgcatt gaggacacag cactcccagg agatcacatc tggtgcgggg gctggttgcc agagcacgat tcagaccaat cgaccccacc tttggtagat 120 180 240 300 360 420 480 540 543 <210> 890 <211> 234 <212> DNA <213> Pinus radiata <400> 890 tacatacaga ttcggatggt ttagcatgaa cctcacgctc gcaccgggac actctgcaag agtggacgat gcttattata tgtcttctaa caccaacatg aatagggacg agctggactt tgagtttctg gggaacagaa gtggagagcc atatgctctg cacacaaaca tctatgcaaa gggtgtgggt ggtagggaac agaggcacat tctctggttc gatccaacga caca <210> 891 <211> 311 <212> DNA <213> Pinus radiata.
<400> 891 tgtttgcaga gatttgttgg ggcaatgctg ctcttggtag ctgcaatggc tgctatggct ttcccaaaaa attatgtacc cacttgggct aatgaggttc agctttctct agacaaatgg tacttgtttg gacactttag tatgcagata 9qyacLqcct t atcaagatgg gcctcacctc gctgatcata acaggtactg aagatggttc tccccaaact ctaagcctgt tcaagtacat gcttccaatc ctggtgactc gttgctggtg ggacgtacca caatggtgga caagggtacc tgcaggcact 120 180 240 300 311 <210> 892 'A7 7 <212> DNA <213> Pinus radiata <400> 892 caacgagagg ctgggagtcc catatcctaa gaaacagccc atgagggtat cctcttcaat ctggaatgca gataactggg ctactcaagg tgggcggctg aagataaact ggagccattc tccttttatc tccacttaca aaaggttcga catcgatgca aaccaatacg gattaaatgg 120 180 WO 00/22092 WO 0022092PCT/NZ99/0OI 69 agaatcaaga gggtttattg agaatggaag taagtggtgg gacaggccct ctcattcttc ccttactcca ttacaaaggc gaatgctccg atgggtgcat cggaactata tcatctatga ctactgcaag gattcgacca ggttttccac ttcgccacct gagtgtgcag gcctccgctt ctagttggtc tctatat <210> 893 <211> 319 <212> DNA <213> Pinus radiata 240 300 360 377 <400> 893 tgtttgcana gatttgttgg ggcaatgctg ctcttggtag ctgcaatggc tgctatggct ttcccaaaaa attatgtacc cacttgggct aatgaggttc agctttctct agacaaatgg tacttgtttg gacactttag tatgcagata gtgactgcct tttatctat atcaagatgg gcctcacctc gctgatcata acaggtactg aagatggttc tccccaaact ctaagcctgt tcaagtacat gcttccaatc ctggtgactc gttgctggtg ggacgtacca caatggtgga caagggtacc tgcaggcact <210> 894 <211> 342 <212> DNA <213> Pinus radiata <400> 894 gggtagcagc gtagtgcaga gcttcttaat tctggctctt tcgagcccag ctgggcgaca tggacaatat ttccggggct gggcacagat taagctcgtt cttctgaggg aactctgcac taagctgt tg tgcttctggg gatcatgtta ggctttgctt ccaggtgact gacgaattcg ttaaaatggc catcccattg tgtatgaggg ccaagacaac ctgcaggcac atttcgaatt ttctttgagt tgcacagttt agagctgttg atatttgttt agttactgct ct atgcagagct aatgatatct aagcttaagc ggaaaagcag ttttatatgt 120 180 240 300 342 <210> <211> <212> <213> 895 529
DNA
Pinus radiata <400> 895 ctggcttcca ttcctggtga acgatgaaat ctaatgtttt ccacaaaaga tggacgatgt ttaaccagcc gtgggctgga acgtggatgg atccaagggt ctctgcaggc agattttgag cagtggagga ttaccatgcc ccccatcaga catgaaaata gaagacagat gtgtgaggcc acctacttgt actgtgactg ttcctgggca aaaggaaaca tactctgtgc gttttcaaga tactcaagcc tggagcaaag tctgtaactg ttggacactt ccttttatct ataggtctgg gagagcagag t ctggaa cat atagcaggga tgtggaatgc caccatttgt agtcgacttg tagtatgcag atcctcccag acagccttac gatatacctc gcatcagatc cttaggagtg tgatgactgg tgcatcatac tgctactca ataaagatgg aatgccgagc attctccaaa tggtttgacc gtattctttg aggtacccat gccacaaggg aggggattcc 120 180 240 300 360 420 480 529 <210> <211> 501 Pinus radiata <400> 896 cgcagctttc tcttgacaaa tggacaggta ctggcttcca atccaagggt agctacttgt ttggacattc agtatgcaga taaagatggt tcctggtgac tctgcaggcg ttgtgactgc cttttattta tcctctcaga actctgaaca tgatgaaata gactttgagt tcttgggcaa taggtctgga caaccttaca ttctccaaac taatgttttc agtggaggaa agggggacag 120 180 240 WO 00/22092 WO 0022092PCT/NZ99/00 169 agagcaacgc gtatatCtCt ggtttgaccc cacaaaagac tatcattcct acactgtcct ttggaatatg catcagattg tattctttgt ggatgatgtc cccatcagag ttttcaagaa cagcaaggat ttaggagtga ggtacccatt caaccagccc atgaaaatct attcaagctt gtggaatgct gatgactggg ctacaagggg tgggttggag aagacagact ggagcaaggc accctttgtt gcatcataca a 300 360 420 480 501 <210> 897 <211> 542 <212> DNA <213> Pinus radiata <400> 897 aattgtttct aatccttgaa caatccaaga aattctgcag atagaccatg ttcgctcaag gcttttcaca attcccgttc cactgtccta gc gcaaatttct aiggccaaca aggagtatct gcacagttac aatttctagg gccttggcaa cttactccgt gtgtgtttaa acagccaagc acaacgacgt attgcagctt gtttgctaaa tgcctattat caacctttcc tcgtgagcaa gctctggaca gaacagggag aatgagggtc cnacgtcaca actctggatc attgatatgc ctgtcgtctc ggagatccct caattctacc ccaaatcaaa acagagttgg tattcaagcc tggggcaatg acacttcagg aaatcaagtt aaggtcccaa atattatgca tgtggt ttga ttacattgtc ctaaagtgga tctggaatgc gtcgcggtaa ttctgggttt ggtacctggc acacgacgaa cactaatatc cccaaccctg tgtggacggg tagcaattat agatgattgg <210> 898 <211> 350 <212> DNA <213> Pinus radiata <400> 898 tggaaatgag gttcagcttt tacctacttg tttggacact cactgtgact gccttttatc gttcctgggc aataggtctg aaaaggaaac agagagcaga ctactctgtg ctctggaaca ctctagacaa ttagtatgca tatcctccca gacagcctta ggatatacct tgcatcaaat atggacaggt gataaagatg gaatgccgag cattctccaa ctggtttgac cgtattcttt actggcttcc gttcctggtg cacgatgaaa actaatgttt cccacaaaag gtggacgatg aatccaaggg actctgcagg tagattttga tcagtggagg attaccatgc <210> 899 <211> 356 <212> DNA <213> Pinus radiata <400> 899 gtttgcacag actcgttggg taggcaatgc tgattaatat gctgctactg ctaccccacc cccacctggg cttctgatca LtgaCaaaLg gacaggtact atatgcagat aaagatggtt tgcttactga ggtgcccaat tccgaagcct tatcaagtac ggcttccaat cctggtgact aagatagcta gtactgttat gtggatgtgc attaatgggg ccaagggtag ctgcaagcgt gcaggaggct ttctcttggt cattccaaaa ggaacgaagc ctacttgttt tgtgactgcc ggtgcttttc agcggcaatq aaactatgta gcactttctc ggacatttca ttttat 120 180 240 300 356 <210> <21>24 <212> DNA <213> Pinus radiata <400> 900 gtgaatgtgc agattaagct Cgttcctgac gactctgctg gcaccgttac tgctttctat atgtcttccc aaggcgatca gcatgacgaa ttcgactttg aatttttggg gaatacttct WO 00/22092 WO 0022092PCTINZ99/00I 69 ggggagccgt acgctgtgca gaccaatgtt ttttctaagg gggttggcaa gcgtgagcag cggattttct tatggttcga cccaaccacg gatttccatt cctattcctt tctctggaaa ccgcagca <210> 901 <211> 376 <212> DNA <213> Pinus radiata 180 240 248 <400> 901 caatggctgc tactgctacc atgtacccac ctgggcttct tttctcttga caaatggaca atttcagtat gcagataaag atttatcctc tcagaactct ctggacaacc ttacattctc aacgcgtata tctctg ccacctccga gatcatatca ggtactggct atggttcctg gaacatgatg caaactaatg agcctgtgga agtacattaa tccaatccaa gtgactctgc aaatagactt ttttcagtgg tgtgccattc tggggggaac gggtagctac aggcgttgtg tgagttcttg aggaaagggg caaaaaaact gaagcgcagc ttgtttggac actgcctttt ggcaataggt gacagagagc 120 180 240 300 360 376 <210> 902 <211> 416 <212> DNA <213> Pinus radiata <400> 902 tttgtttgca cagactcgtt ttctaggcaa tgctgatcaa atggctgcta ctgctacccc gtacccacct gggcttctga tctcttgaca aatggacagg ttcagtatgc agataaagat ttatcctctc agaactctga gggtgcttac tatggtgccc acctccgaag tcatatcaag tactggcttc ggttcctggt acatgatgaa tgaaagatag aatgtactgt cctgtggatg tacattaatg caatccaagg gactctgcag atagactttg ctagcaggag tatttctctt tgccattcca gggggaacga gtagctactt gcgttgtgac agttcttggg gctggtgctt ggtagcggca aaaaaactat agcgcagctt gtttggacat tgccttttat caatag <210> 903 <211> 417 <212> DNA <213> Pinus radiata <400> 903 tcgtgccgaa ttcggcagag ttgaatactq atctaaagaa aactggaaga aaaccattgg cctacaaaac accagtatga gggcttggag gagaatttaa agctgtcatc acgagctgga aacactggag atgcccaaac ctttgtcttc ggagcttgac cttcaatggg t tgggatgct attaaatgtt aatagcacag aggctgggat tggggtggaa cat at ggcc a acccttctaa gcaacaacta gagtgcaatc atttatggga actgatcaat gagaaggtta aatttcttca ttgagcctaa tgggcttcct atgcaaccct tgcttggaaa ttcttacgga ccaaactctc agcggccgtg gccacaagaa acaaagatat ctctgggcac cattgatgca tattgga 120 180 240 300 360 417 <211> 314 <212> DNA <213> Pinus raitA <400> 904 aaacactgga gatgcccaga caggttggga agcaactttg atcatgttga ctgttataaa ctttgatgca aaactgagga gggaaagtgt atcagggatg gacactttgg cgcgaggatt aacattaaca gagcttgttc gaaagcgtta tactgatcaa aaa tggaggg agatgtagaa gagaaatgca tgaatccttt tttctcacag atattagtga cttgcacctg gtggtttcaa gatctgttca ttgctcatat gctcaactgc tgcaggatgg gattcaaaat tgggagcttc wo 00/22092 WO 0022092PCT/NZ99/O01 69 aattgaggaa ggca <210> 905 <211> 323 <212> DNA <213> Pinus radiata <400> 905 aaacactgga gatgcccaga agcaactttg atcatgttga ctttgatgca aaactgagga atcagggatg gacactttgg aacattaaca gagcttgttc aattgaggaa ggcagtctca caggttggga ctgtcataaa gggaaagtgt cgcgaggatt gaaagcgtta gct tactgatcaa aaatggaggg agatgtagaa gagaaatgca tgaatccttt tttctcacag atattagtga cttgcacctg gtggtttcaa gatctgttca ttgctcatat gctcaactgc tgcaggatgg gattcaaaat tgggagcttc <210> 906 <211> 421 <212> DNA <213> Pinus radiata <400> 906 aaagatatgg gcttggagga ctgggcacag ctgtcatcac ttgatgcaaa cactggagat ttggagaagc aactttaatc ggttcaactt tgatgcaaaa ctcatatatc gggcatggac aggatggaag aatgccagag g gaatttaaat gagctggaaa gcccaaacag atgttgactg t tgcggagag actcttgcac cttgttgcaa taaatgttga tagcacagat gctgggatac ttataaagaa agagtgtaga ggggcttgag aacgttatga gtgcaatcat ttatgggatg tgatcaattt tggtgggct t tgtggaagat aaatgcggct aaccttcaat gcaaccctct cttggaaaca cttacggata gcacctggtg ctattcattg aagctgttgc tcagaactag 120 180 240 300 360 420 421 <210> 907 <211> 530 <212> DNA <213> Pinus radiata <400> 907 gagaggtggt aatgccaatc cgtcctctgt acaagtccag gtgacaaatg actttgctga gcagtcaatt caagaaccta agatatggcc gctttcatga tagatgctgt ggggaactgc atatgcatgt aactaggggg atactgatct ggaagaagaa caaaacacca ttgcagatga ccgggatatt tgttgctctt ccagttgttt ctatgcatat agagaatttt gaaaaaggaa tataggcttc gtatgactgg atttaaattg gctcctgatq gccaaacagc atgcatcccc gctgcagctc gttttctggg cttgaccata aatggaactc gatgctgcaa aacattgagt gaagaacact ttcaggaagg gctacatgca aagtcaagaa gtggaagaga tggctaggtt ttctaattga c aa ca at ggg gcaaccatgc tgcagaatca aactgaaatt tggagctgct agctatggag gggctatcaa tctacaagca acccaaacca tttcctgcag 120 180 240 300 360 420 480 530 210 i <211> <212> <213> 908 373
DNA
Pinus radiata <400> 908 caatgggacc Cttctaattg agcctaagcc ggatgctgca acaactatgg gcttcctaca aaatgttgag tgcaatcatg caaccctctc agcacagatt tatgggatgc ttggaaacat ctgggatact gatcaatttc ttacggatat acaagaacct aagatatggg tgggcacagc tgatgcaaac tggagaagca acaaaacacc cttggaggag tgtcatcacg actggagatg actttaatca agtatgattg aatttaaatt agctggaaat cccaaacagg tgttgactgt 120 180 240 300 WO 00/22092 PCT/NZ99/001 69 tataaagaat ggtgggcttg cacctggtgg gttcaacttt gatgcaaaat tgcggagaga 360 gagtgtagat gtg 373

Claims (2)

  1. 2. Se-p. 2004 11:34 Sheiston IP No. 1311 P. -32- THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:- 1. An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: sequences recited in SEQ ID NOS: 15-19, 71-78, 105, 139, 143 or 552- 555; complements of the sequences recited in SEQ ID NOS: 15-19, 71-78, 105, 13 9, 143 or 5 52-5 reverse complements of thc sequences recited in SEQ ID NOS: 15-19,
  2. 71-78, 105, 139, 143 or 552-555; reverse sequences of the sequences recited in SEQ ID NOS: 15-19, 71- 78, 105, 139, 143 or 552-555; nucleotide sequences having at least 90% identity to a sequence of SEQ ID NO: 15-19, 71-78, 105, 139,143 or 552-555; nucleotide sequences having at least 75% identity to a sequence of SEQ IDNO: 15-1971,.72, 74-78, 105, 139, 143, 552, and555; nucleotide sequences that hybridise to a sequence of SEQ ID NO: 15-19, 71, 72, 74-78, 105, 139, 143, 552, 554 and 555 above under stringent hybridisation conditions; (hi) nucleotide sequences that are 200-mers of a sequence of SEQ ID NO: 19, 71-78, 105, 139, 143 and 552-555; nucleotide sequences that are 1 00-mers of a sequence of SEQ ED) NO: 19, 71-78, 105, 139, 143 and 552-555; nucleotide sequences that are degenteratively equivalent to a sequence of SEQ ID NO: 15- 19, 71-78, 105, 139, 143 and 5 52-55 5; and nucleotide sequences that are allclic variants of a sequence of SEQ ID NO: 15-19, 71-78, 105, 139,143 and 552-555. 2. An isolated oligonucleotide, probe or primer comprising at least 20 contiguous reWwMejhffatcFyT 20 vonguwreghnaf notder-scquencedefned in~ clim 1. 3. A kit comprising a plurality of oligonucleotide probes or primers of claim 2. 4. A strg edium ha n rorded thereon a plurality o-f p olynuclectides, at least one of the polynueleotides comprising a nucleotide sequence defined in claims 1 or 2. A genetic construct comprising a polynucleotide of claim 1. COMSID No: SBMI-00X896429 Received by IP Australia: Time 11:35 Date 2004-09-02 -33- 6. A transgenic cell comprising a construct according to claim 7. A genetic construct comprising, in the direction: a gene promoter sequence; a polynucleotide sequence comprising at least one of the following: a polynucleotide coding for at least a functional portion of a polypeptide encoded by a nucleotide sequence of claim 1; and a polynucleotide comprising a non-coding region of a gene coding for a polypeptide encoded by a nucleotide sequence defined in claim 1; and a gene termination sequence. 8. The construct of claim 7, wherein the polynucleotide sequence is in a sense orientation. 9. The construct of claim 7, wherein the polynucleotide sequence is in an antisense orientation. The construct of claim 7, wherein the gene promoter sequence is functional in a plant host to provide for transcription in xylem. 11. A transgenic plant cell comprising a construct of claim 7. 12. A transgenic plant comprising a transgenic plant cell according to claim 11, or a part or propagule or progeny thereof. 13. A method for modulating one or more of the polysaccharide content, the polysaccharide composition and the polysaccharide structure of a plant, comprising stably incorporating into the genome of the plant a polynucleotide of claim 1. 14. The method of claim 13 wherein the plant is selected from the group consisting V*0: of eucalyptus and pine species. 15. The method of claim 13 comprising stably incorporating into the genome of the 25 plant a construct of claim 7. 16. A method for producing a plant having one or more of altered polysaccharide content, altered poiysaccharide composition and altered polysaccharide structure, comprising: transforming a plant cell with a construct of claim 7 to provide a 30 transgenic cell; and cultivating the transgenic cell under conditions conducive to regeneration S"and mature plant growth. 500363762 .DOC/BSW -34- 17. A method for modifying the activity of a polypeptide involved in a polysaccharide biosynthetic pathway in a plant comprising stably incorporating into the genome of the plant a construct of claim 7. 18. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: SEQ ID NOS: 44-48, 95-102, 106, 144 and 148; sequences having at least 70% identity to a sequence of SEQ ID NO: 44, 46-48, 95, 96, 98, 100-102, 106 and 144; sequences having at least 75% identity to a sequence of SEQ ID NO: 44, 46-48, 95, 96, 98, 100-102, 106 and 144; and sequences having at least 90% identity to a sequence of SEQ ID NO: 44- 48, 95-102, 106, 144 and 148. 19. An isolated polypeptide encoded by an isolated polynucleotide sequence of claim 1. An isolated polynucleotide according to claim 1, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 21. An isolated oligonucleotide probe or primer according to claim 2, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 22. A kit according to claim 3, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 23. A storage medium according to claim 4, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the 25 accompanying drawings and/or examples. 24. A genetic construct according to claim 7, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 25. A transgenic plant cell according to claim 11, substantially as herein described 30 with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 500363762 .Doc/BSW 26. A transgenic plant according to claim 12, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 27. A method according to any one of claims 13, 16, or 17, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. 28. An isolated polypeptide according to claim 18 or 19, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples. DATED this 10 t h day of June 2004. BALDWIN SHELSTON WATERS Attorneys for: 9* 00 500363762 I.DOC/BSW
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US7674951B1 (en) 1999-05-21 2010-03-09 Michigan Technological University Isolated cellulose synthase promoter regions
US7049481B1 (en) 1999-05-21 2006-05-23 Board Of Control Of Michigan Technological University Cellulose synthase encoding polynucleotides and uses thereof
US20020064816A1 (en) * 1999-12-16 2002-05-30 Jens Lerchl Moss genes from physcomitrella patens encoding proteins involved in the synthesis of carbohydrates
EP1138771A1 (en) * 2000-03-30 2001-10-04 Societe Des Produits Nestle S.A. Coffee endo-mannanase
PT102511B (en) * 2000-08-22 2007-08-01 Inst De Ciencia Aplic E Tecnol B-GALACTOSIDASE ENCODING GENES, PECTINAMETHYSTERASE, POLIGALACTURONASE PEX ISOLATED EEXPANSINS
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WO2004013304A2 (en) 2002-08-02 2004-02-12 Basf Plant Science Gmbh Sugar and lipid metabolism regulators in plants iv
WO2005001051A2 (en) * 2003-06-06 2005-01-06 Arborgen Llc. Compositions and methods for regulating polysaccharides of a plant cell
WO2007019245A2 (en) 2005-08-03 2007-02-15 Board Of Control Of Michigan Technological University Methods for enhancing expression of secondary cell wall cellulose synthases in plants
EP2779825A4 (en) * 2011-11-16 2015-07-01 Israel State Use of fructokinases and sucrose synthases for increasing cell wall polymers
CN105925605A (en) * 2016-06-03 2016-09-07 华南农业大学 Application of lotus cellulose synthase gene NnuCESA8
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